RESUMO Introdução: A síndrome da fibromialgia (FM) é caracterizada pela presença de dor difusa com duração de mais de três meses e, frequentemente, é associada a distúrbios do sono. Estudos investigaram o efeito do treinamento de força (ST) sobre a dor e a qualidade do sono dos pacientes com FM, mas ainda existem diversas perspectivas quanto aos efeitos da intervenção nessa população. Objetivos: O objetivo do estudo foi examinar os efeitos do treinamento de força (ST) sobre a dor e a qualidade do sono de pacientes com FM. Métodos: Quarenta e oito mulheres com FM participaram do estudo entre agosto e outubro de 2012. Seis (55 ± 6,5 anos) realizaram treinamento de força, conduzido na Universidade Estadual de Santa Catarina, e oito (47 ± 9 anos) constituíram o grupo controle. Foram empregados o Questionário Sociodemográfico e Clínico, a escala visual analógica (EVA) para dor e o Índice de Qualidade do Sono de Pittsburgh. Os dados foram coletados antes da primeira sessão e depois da intervenção de oito semanas e foram analisados por meio de estatística descritiva e testes inferenciais. Resultados: A intervenção de 8 semanas com ST diminuiu a dor (p < 0,05) e reduziu significativamente as disfunções diurnas do sono (p <0,05), demonstrando que o programa proposto contribui para melhorar a qualidade de vida dos pacientes. Conclusão: O ST é um tratamento viável para pacientes com fibromialgia. Nível de evidência II; Estudo terapêutico.

ABSTRACT Introduction: Fibromyalgia syndrome (FM) is characterized by the presence of diffuse pain lasting for more than three months and is often associated with sleep disorders. Studies have investigated the effect of strength training (ST) on pain and sleep quality in FM patients, but there continue to be diverse perspectives on the effects of this intervention in this population. Objective: The aim of the study was to examine the effects of strength training (ST) on pain and sleep quality in FM patients. Methods: Forty-eight women with FM participated in the study between August and October, 2012. Six (55±6.5 years) performed ST, conducted at Santa Catarina State University, and eight (47±9 years) comprised the control group. The Socio-Demographic and Clinical Questionnaire, the visual analog scale (VAS) for pain, and the Pittsburgh Sleep Quality Index were used. Data were collected before the first session and after the eight-week intervention and were analyzed using descriptive statistics and inferential tests. Results: The eight- week ST intervention decreased pain (p< 0.05) and significantly diminished the daytime sleep dysfunctions (p< 0.05), demonstrating that the proposed program contributes to improving patient quality of life. Conclusion: ST is a feasible treatment for patients with fibromyalgia. Level of evidence II; Therapeutic study.

RESUMEN Introducción: El síndrome de fibromialgia (FM) se caracteriza por la presencia de dolor difuso de más de tres meses de duración y suele asociarse a trastornos del sueño. Los estudios han investigado el efecto del entrenamiento de fuerza (ST) sobre el dolor y la calidad del sueño en pacientes con FM, pero todavía existen diversas perspectivas respecto a los efectos de esta intervención en esta población. Objetivos: El objetivo del estudio fue examinar los efectos del entrenamiento de fuerza sobre el dolor y la calidad del sueño de los pacientes con FM. Métodos: Cuarenta y ocho mujeres con FM participaron en el estudio entre agosto y octubre de 2012. Seis (55 ± 6,5 años) se sometieron a un entrenamiento de fuerza realizado en la Universidad del Estado de Santa Catarina, y ocho (47 ± 9 años) constituyeron el grupo de control. Se utilizó el Cuestionario sociodemográfico y Clínico, la escala visual analógica (EVA) para el dolor y el Índice de Calidad del Sueño de Pittsburgh. Los datos fueron recopilados antes de la primera sesión y después de la intervención de ocho semanas y se analizaron mediante estadísticas descriptivas y pruebas de inferencia. Resultados: La intervención de ocho semanas con ST disminuyó el dolor (p <0,05) y redujo significativamente los trastornos del sueño durante el día (p <0,05), lo que demuestra que el programa propuesto contribuye a mejorar la calidad de vida de los pacientes. Conclusión: El entrenamiento de fuerza es un tratamiento viable para pacientes con fibromialgia. Nivel de evidencia II; Estudios terapéuticos.

ABSTRACT In the world the consumption of strawberries is very appreciated, both in natura or industrialized form. Brazilian production depends on the importation of strawberry seedlings from countries such as Spain, Chile and Argentina, which are cultivars improved in temperate climates and, consequently, not well adapted to the Brazilian tropical conditions. Thus, genetic breeding programs are essential for the generation of more adapted cultivars. The objective was to evaluate and characterize the performance of experimental strawberry genotypes. The statistical design adopted was in randomized blocks, with 12 treatments and 3 replications, the treatments consisting of seven experimental genotypes and five commercial cultivars. We evaluated the total production per plant (g/plant), average weight of fruits per plant (g/fruit), % of commercial fruits per plant, and morphological characterization. The experimental genotypes MFA443, MCA94, MDA23, MDA06 and MCA89 showed the highest strawberry yields per plant, respectively, 285 g, 254 g, 298 g, 295 g and 290 g, with emphasis on the genotype MFA12-443, which also presented the highest average weight of fruits (20.35 g) and percentage of commercial fruits (81%).

RESUMO Mundialmente o consumo de morangos é bastante apreciado, tanto na forma in natura ou industrializada. A produção brasileira depende da importação de mudas de morangueiro de países como Espanha, Chile e Argentina, as quais são cultivares melhoradas em climas temperados, e, consequentemente, não se adaptam bem às condições tropicais brasileiras. Assim, programas de melhoramento genético são fundamentais para geração de cultivares mais adaptadas. Objetivou-se avaliar e caracterizar o desempenho de genótipos experimentais de morangueiro. O delineamento experimental adotado foi em blocos cazualizados, com 12 tratamentos e 3 repetições, sendo os tratamentos constituídos de sete genótipos experimentais e cinco cultivares comerciais. Avaliou-se os parâmetros: produção total por planta (g/planta), peso médio de frutos por planta (g/fruto), % de frutos comerciais por planta, e caracterização morfológica. Os genótipos experimentais MFA12-443, MCA94, MDA23, MDA06 e MCA89 apresentaram as maiores produções de frutos por planta, respectivamente de 285 g, 254 g, 298 g, 295 g e 290 g, com destaque para o genótipo MFA12-443, que também apresentou maior peso médio de frutos (20,35 g) e porcentagem de frutos comerciais (81%).

RESUMO Há grande demanda de novas cultivares adaptadas para a produção em clima nacional, o que cria certa dependência dos produtores de morango nacionais às cultivares importadas. Com isso, o objetivo do trabalho foi avaliar características de qualidade e morfológicas de frutas provenientes de genótipos de morangueiro. Os acessos de morangueiro foram cedidos pelo Banco de Germoplasma da Universidade Federal de Lavras. O experimento foi implantado no setor de Experimentação e de Olericultura da Universidade José do Rosário Vellano (UNIFENAS). O delineamento experimental utilizado foi em blocos casualizados, com 12 tratamentos e 3 repetições, totalizando 36 unidades experimentais. Os 12 tratamentos foram constituídos de sete genótipos e cinco cultivares comerciais posicionadas em 29 slabs, em cultivo protegido. Os genótipos MFA12-443 e MCA12-89 destacaram-se no tamanho de seus frutos (p<0,000), apresentando os maiores valores de comprimento (41,06 mm), largura (32,60 mm) e espessura (27,84 mm), enquanto não se diferenciaram entre si para estes valores. Estes genótipos apresentam melhor aptidão para o mercado in natura, com características de qualidade mais apreciadas pelos consumidores. Todos os genótipos apresentaram níveis maiores que 7ºBrix (p= 0,000) indicados para a comercialização no mercado. Os acessos testados apresentaram qualidade e aptidão para a produção na região do sul de Minas Gerais.

ABSTRACT There is great demand for new cultivars adapted for production in a national climate, which creates a certain dependence of national strawberry growers on imported cultivars. Thus, the objective of this work was to evaluate quality and morphological characteristics of fruits from strawberry genotypes. Strawberry accessions were provided by the Germplasm Bank of the Federal University of Lavras. The experiment was implemented in the Experimentation and Olericulture sector of the José do Rosário Vellano University (UNIFENAS). The experimental design used was in randomized blocks, with 12 treatments and 3 replications, totaling 36 experimental units. The 12 treatments consisted of seven genotypes and five commercial cultivars positioned in 29 slabs, in protected cultivation. The genotypes MFA12-443 and MCA12-89 stood out in terms of fruit size (p<0.000), presenting the highest values for length (41.06 mm), width (32.60 mm) and thickness (27.84 mm), while not differed from each other for these values. These genotypes are better suited for the fresh market, with quality characteristics that are more appreciated by consumers. All genotypes presented higher levels than 7ºBrix (p= 0.000) indicated for commercialization in the market. The tested accessions showed quality and aptitude for production in the southern region of Minas Gerais.

Resumo Objetivo Caracterizar o perfil e identificar os fatores associados à Hepatite B na população com 60 anos ou mais de idade da região Sudoeste do Paraná, Brasil. Método Trata-se de um estudo epidemiológico descritivo e inferencial que analisou quantitativamente dados secundários obtidos através do Sistema Nacional de Agravos de Notificação (SINAN), entre 2007 a 2017. Resultados A Hepatite B foi mais frequente em homens, com baixa escolaridade e em indivíduos que exerciam atividade ligada à agricultura. A maioria dos casos foi notificada entre 2013 a 2017 e em sujeitos que reportaram exposição a material biológico. No modelo multivariado de regressão logística, as infecções pelos vírus da hepatite B foram associadas à raça/cor não branca (OR: 2,89; IC95% 1,07 – 7,87), ao histórico de realização de transfusão sanguínea (OR: 14,51; IC95% 5,44 – 38,74), em residentes de municípios de 10 a 20 mil habitantes (OR: 4,57; IC95% 1,59 – 13,12) e de municípios entre 20 a 50 mil habitantes (OR: 4,33; IC95% 1,61 – 11,56). Conclusão A caracterização dos possíveis fatores de risco para hepatite B nessa população podem subsidiar ações mais eficazes de prevenção e promoção de saúde, bem como fomentar estudos específicos que possam guiar políticas de atenção integral ao idoso.

Abstract Objective to characterize the epidemiologic profile of Hepatitis B in the population aged 60 years old or more from the southwest region of the state of Paraná, Brazil, between 2007 and 2017. Method an epidemiologic, descriptive, and inferential study was conducted based on notifications obtained from the Notifiable Diseases Information System(or SINAN). Results Hepatitis B was more frequent in men, those with low levels of schooling and among individuals who worked in agriculture. Most of the cases were reported between 2013 and 2017 in subjects who reported having been exposed to biological material. According to logistic regression, hepatitis B infections were associated with non-white ethnicity (OR: 2.89; 95%CI 1.07 – 7.87), a history of blood transfusions (OR: 14.51; 95%CI 5.44 – 38.74), living in municipal regions with 10,000 to 20,000 inhabitants (OR: 4.57; 95%CI 1.59 – 13.12) and also among individuals from municipal regions with 20,000 to 50,000 inhabitants (OR: 4.33; 95%CI 1.61 – 11.56). Conclusion the epidemiological profile reported here represents a risk factor for hepatitis B in this population. The data can support more effective interventions, as well as further studies to guide comprehensive public health policies for older adults.

Resumo Objetivo Analisar se a redução no tempo de espera para cirurgia ortopédica nos pacientes idosos com fratura da extremidade proximal do fêmur impacta na redução da mortalidade intra-hospitalar, da mortalidade em 6 meses de seguimento, e na redução do tempo de internação hospitalar. Métodos Trabalho de coorte retrospectiva fundamentado na análise de prontuários de 81 pacientes internados com diagnóstico de fratura transtrocanteriana ou subtrocanteriana do fêmur, e submetidos a tratamento cirúrgico em 2015 e 2016 em um hospital de referência no atendimento ao trauma, antes e após a implantação de protocolo para cirurgia em até 48 horas. Resultados Observou-se redução do tempo médio de internação de 17 para 11 dias após a implantação do protocolo. Com relação à mortalidade intra-hospitalar, cinco pacientes faleceram antes da implantação do protocolo, e cinco, após a implantação do protocolo. Referente à mortalidade extra-hospitalar, avaliada em 6 meses de pósoperatório, observou-se uma redução de 26,7% para 19,4%. Os resultados não apresentaram significância estatística. Conclusão Opresente estudodemonstrouque existe uma tendência à redução dotempo de internação hospitalar e damortalidadeem6meses quando a cirurgia para tratamento de fraturas do fêmur proximal no idoso é feita em até 48 horas de internação hospitalar.

Abstract Objective To analyze whether a reduction in the waiting time for orthopedic surgery in elderly patients with fracture of the proximal end of the femur leads to a reduction in in-hospital mortality, 6-month mortality, and duration of hospitalization. Methods This was a retrospective cohort study including 81 patients with intertrochanteric and subtrochanteric fractures who underwent surgical repair between 2015 and 2016 in a referral hospital, before and after a protocol for early surgical fixation (< 48 hours) was implemented. Results Themean length of hospital stay decreased from17 to 11 days. Regarding the in-hospital mortality rate, five patients died before, and another five died after the protocol was implemented. The 6-month postoperative mortality decreased from 26.7% to 19.4%. The results showed no statistical significance. Conclusion The present study demonstrated that there is a tendency to reduce the length of hospital stay and 6-month mortality when the surgery for the treatment of proximal femoral fractures in the elderly is performed within 48 hours of hospitalization.

RESUMO O objetivo do estudo foi avaliar o humor de atletas profissionais de futsal durante a segunda fase da Liga Futsal Brasileira 2012. A amostra foi composta por 15 atletas do sexo masculino e o instrumento utilizado para avaliação foi a Escala de Humor de Brunel. Os resultados mostram que houve diferenças significativas (p<0,05) dos estados de humor no dia anterior, pré-jogo e pós-jogo. A equipe apresentou estados de humor correspondente ao perfil iceberg em quase todos os momentos: vigor constante e elevado, com exceção do pós-jogo nas derrotas; e fadiga com índices baixos, que se apresentaram elevadas no pós-jogo. Conclui-se que os estados de humor podem intervir positivamente ou negativamente no desempenho esportivo da equipe.

ABSTRACT This study evaluated the moods states of professional futsal players during the second phase of the Brazilian Futsal League 2012. The sample was composed of 15 male athletes and the instrument used for evaluation was the Brunel Mood Scale. The results show that there were significant differences (p<0.05) in mood states on the previous day, pre-game and post-game. The team presented mood states corresponding to the iceberg profile in almost all times: constant and high vigor, except for the post-game defeats, and fatigue with low levels, which were elevated in the post-game. It was concluded that mood states can positively or negatively interfere with team sports performance.

Abstract Purpose: To investigate cardiac changes in young rats, whose mothers underwent autogenic fecal peritonitis, during organogenesis phase and to evaluate the role of intravenous administration of moxifloxacin and dexamethasone in preventing infection-related cardiac changes. Methods: A prospective histomorphometric study was performed on 29 hearts of Wistar four-month old rats. Animals were divided into three groups: Negative Control Group (NCG) included 9 subjects from healthy mothers; Positive Control Group (PCG) included 10 subjects from mothers with fecal peritonitis (intra-abdominal injection of 10% autogenic fecal suspension in the gestational period) and did not receive any treatment; and Intervention Group (IG), with 10 animals whose infected mothers received moxifloxacin and dexamethasone treatment 24 hours after induction of fecal peritonitis. Results: Nuclear count was higher in the IG group as compared to PCG (p = 0.0016) and in NCG as compared to PCG (p = 0.0380). There was no significant difference in nuclear counts between NCG and IG. Conclusion: Induced autogenic fecal peritonitis in pregnant Wistar rats determined myocardial changes in young rats that could be avoided by the early administration of intravenous moxifloxacin and dexamethasone.

Abstract Purpose: To evaluate the response of aging rats with sepsis to two different antibiotic regimens. Methods: The study was conducted with 30 aging rats (18 month-old) with autologous feces peritonitis. The animals were divided into three groups: Group 0 received no therapeutic intervention (control), while Group 1 received a single dose of 40 mg/kg meropenem and Group 2 received a single dose of 20 mg/kg moxifloxacin. The intervention in both Groups was made 6 hours after induction of peritonitis. The animals were followed up to 15 days for evaluating morbidity and mortality. The weights at baseline were similar in all groups. Results: At the end of follow-up, weight loss was significantly greater (p=0.0045) in Group 0 (non-intervention controls). Culture from a blood sample at the end of follow-up was positive in all the animals in Group 0, in two animals in Group 1 and in four animals in Group 2. Morbidity/mortality was significantly higher in Group 0 compared to both Groups 1 and 2 (p=0.003) but the scores were not significantly different between Groups 1 and 2 (p=0.6967). Conclusion: Both antibiotic regimens rendered promising results for the treatment of fecal peritonitis.

PURPOSE: To evaluate the treatment outcome of severe peritonitis in rats with increasing age.METHODS:Thirty Wistar rats stratified in three groups: group I - six month-old; group II - 12 month-old; and group III - 18 month-old, underwent autogenously fecal peritonitis (6 ml/kg rat), and were treated with intravenous meropenem. The survival animals were followed-up for 45 days. The variables were expressed by their mean and standard error of the mean (SEM). p<0.05 was used for rejecting the null hypothesis. The study was approved by the Ethics Committee.RESULTS: There was a significant increase in the mortality and morbidity in elderly rats. Of interest, even among young survival rats presenting with severe residual abscesses both in the abdomen and thorax cavities, they present an almost normal life.CONCLUSIONS:The treatment of severe autogenously fecal peritonitis with intravenous meropenem reached reasonable results in rats with six and twelve months of age, even considering residual abscesses on abdomen and thorax cavities. However, the great majority (80%) of elderly rats could not overcome the initial severe infectious challenge, proving that ageing is a very important risk factor for impairing immune response. Thus, sepsis remains a challenging situation, especially in elderly.

INTRODUÇÃO: A saúde mental de atletas pode predizer seu desempenho esportivo e comportamental, o que a torna um importante objeto de estudo.OBJETIVO: Investigar a saúde mental e fatores associados em atletas que participaram dos Jogos Abertos de Santa Catarina 2011, Brasil.MÉTODOS: A amostra foi composta por 445 atletas participantes da competição (211 homens e 234 mulheres). Para a coleta de dados foi utilizado o questionário de caracterização e a escala de humor de Brunel. Os dados foram coletados individualmente no local da competição entre 30 e 50 minutos antes dos atletas competirem. Os dados foram analisados por estatística descritiva (média, frequência e percentual) e inferencial (Kruskal-Wallis, Mann-Withney e correlação de Spearman com α estabelecido de 0,05).RESULTADOS: Os atletas competiram em 10 diferentes modalidades, coletivas e individuais. A maioria dos atletas cursa ou cursou o ensino médio ou superior, com mais de sete anos de tempo de prática e participação em competições nacionais e estaduais. Grande parte dos atletas teve percepção de qualidade de descanso e sono entre boa e regular durante a competição. De maneira geral, os atletas apresentaram boa saúde mental, com perfil semelhante ao de iceberg (elevado vigor, níveis moderados de tensão e baixa fadiga, depressão, raiva e confusão mental). Os atletas com melhor percepção de sono e saúde que não fazem uso de medicamentos reguladores de humor apresentam melhor saúde mental.CONCLUSÃO: Torna-se evidente a boa qualidade de saúde entre os atletas participantes do estudo, destacando-se um cuidado especial com relação às implicações da qualidade do sono e o uso de medicamentos reguladores de humor na saúde mental dos atletas.

INTRODUCTION: Mental health of athletes can predict their sports and behavioral performance, making it an important field to explore.OBJECTIVE: The aim of this study was to investigate the mental health and associated factors in athletes who participated in the Santa Catarina Open Games of 2011, Brazil.METHODS: The sample consisted of 445 athletes who participated in the competition (211 men and 234 women). For the data collection the Questionnaire of Characterization and the Brunel Mood Scale were used. Data were collected individually at the competition between 30 and 50 minutes before the athletes compete. Data were analyzed using descriptive statistics (mean, frequency and percentage) and inferential statistics (Kruskal-Wallis, Mann Whitney and Spearman correlation with α set at 0.05).RESULTS: The athletes competed in 10 different sports, individual and collective. Most athletes are high school students or attended high school or university, with over than seven years of practice and participation in state and national competitions. Most athletes had perceived quality of rest and sleep between good and regular during the competition. In general, the athletes had good mental health, with a profile similar to an iceberg (high vigor, moderate levels of stress and low fatigue, depression, anger and confusion). Athletes with a better perception of sleep and health that do not use mood regulators have better mental health.CONCLUSION: It is evident the good quality of health among athletes participating in this study, highlighting a special care regarding the implications of sleep quality and the use of mood regulators in the mental health of athletes.

INTRODUCCIÓN: La salud mental de atletas puede predecir su desempeño deportivo y comportamental, lo que la vuelve un importante objeto de estudio.OBJETIVO: Investigar la salud mental y factores asociados en atletas que participaron en los Juegos Abiertos de Santa Catarina 2011, Brazil.MÉTODOS: La muestra fue compuesta por 445 atletas participantes de la competencia (211 hombres y 234 mujeres). Para la colecta de datos fue utilizado el Cuestionario de Caracterización y la Escala de Humor de Brunel. Los datos fueron colectados individualmente en el local de la competencia entre 30 y 50 minutos antes de que los atletas compitieran. Los datos fueron analizados por estadística descriptiva (promedio, frecuencia y porcentual) e inferencial (Kruskal-Wallis, Mann-Withney y correlación de Spearman con α establecido de 0,05).RESULTADOS: Los atletas compitieron en 10 diferentes modalidades, colectivas e individuales. La mayoría de los atletas cursa o cursó la enseñanza media o superior, con más de siete años de tiempo de práctica y participación en competencias nacionales y estaduales. Gran parte de los atletas tuvo percepción de calidad de descanso y sueño entre buena y regular durante la competencia. De manera general, los atletas presentaron buena salud mental, con perfil semejante al de iceberg (elevado vigor, niveles moderados de tensión y baja fatiga, depresión, rabia y confusión mental). Los atletas con mejor percepción de sueño y salud que no hacen uso de medicamentos reguladores de humor presentan mejor salud mental.CONCLUSIÓN: Se ha vuelto evidente la buena calidad de salud entre los atletas participantes del estudio, destacándose un cuidado especial con relación a las implicaciones de la calidad del sueño y el uso de medicamentos reguladores de humor en la salud mental de los atletas.

PURPOSE: To evaluate the effects of chronic cerebral hypoxia on memory of rats submitted to bilateral common carotid artery ligation (BCCL).METHODS: Every each week, for 16 weeks, 31 rats were tested for memory using a water and land mazes and compared with 30 normal rats (control group A). The variables were expressed by their mean and standard error of the mean (SEM). p<0.05 was used for rejecting the null hypothesis. The study was approved by the Ethics Committee for animal investigation.RESULTS: There was a significant increase in the latency time, in the survival water and land mazes, after four weeks (study group B) follow-up. However, without any medication or therapeutically induced measures, after 16 weeks (study group C) follow-up the latency mean times tend to be similar to control group (A) in the neurocognitive tests.CONCLUSIONS: Neurocognitive deficits after 16 weeks post-operative follow-up of rats that underwent bilateral common carotid artery ligation is a natural adaptive phenomenon. Thus, is not realistic to allow translational information from this animal model for therapeutically approaches aiming at to prevent, or to improve brain damage in human beings suffering from chronic deprivation of adequate blood supply.

PURPOSE: To evaluate the effect of cerebral hypoxia-ischemia on memory and learning survival of rats submitted to permanent bilateral carotid ligation (PBCL). METHODS: Twenty-four survivors of PBCL were evaluated after 30 days with regard to memory and learning using a water survival maze. Twenty-three healthy rats were used as control group. The results were expressed by their means and standard error of the mean (SEM). p<0.05 was used for rejecting the null hypothesis. The study was approved by the Ethics Committee for animal investigation. RESULTS: The mortality rate for the surgery was 44.4%. The latency time to find the survival platform was higher in rats that underwent PBCL (Normal: 10.24 ± 1.85s - Study: 25.30 ± 4.69s - Mann - Whitney p=0.0388). Additionally, the type of swimming and the spatial stability of the studied rats on the survival platform were compromised in these animals. CONCLUSION: The permanent bilateral carotid ligation induces change in the learning and survival memory.

OBJETIVO: Estabelecer o método de isolamento e cultivo das neuroesferas de glioblastoma humano, bem como purificação de suas células-tronco, seguido do processo de obtenção de subesferas tumorais, caracterizando imunofenotipicamente esse conjunto clonogênico. MÉTODOS: Por meio do processamento de amostras de glioblastomas (n=3), cumpriu-se a seguinte estratégia de ação: (i) estabelecimento da cultura primária de glioblastoma; (ii) isolamento e cultura de neuroesferas tumorais; (iii) purificação das células que iniciam os tumores (CD133+) por sistema de separação magnética (MACS); (iv) obtenção subesferas tumorais; (v) estudo da expressão de marcadores GFAP, CD133 e nestina. RESULTADOS: Este estudo descreveu com sucesso o processo de isolamento e cultivo de subesferas de glioblastoma, as quais são constituídas por um conjunto clonogênico de células caracterizadas imunofenotipicamente como neurais, capazes de iniciar a formação tumoral. CONCLUSÃO: Estes achados poderão contribuir para a compreensão do processo de gliomagênese.

OBJECTIVE: To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set. METHODS: Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133+) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the expression of the markers nestin, CD133, and GFAP. RESULTS: The study successfully described the process of isolation and culture of glioblastoma subspheres, which consist of a number of clonogenic cells immunophenotypically characterized as neural, which are able to initiate tumor formation. CONCLUSION: These findings may contribute to a better understanding of the process of gliomagenesis.

Os eventos e reações envolvendo quimioterapia são frequentes na prática oncológica. Agentes quimioterápicos são uma modalidade de tratamento amplamente utilizada. Efeitos colaterais podem variar de frequência e também ser confundidos com outras manifestações tegumentares do tratamento oncológico. Este artigo objetiva expor as informações sobre reações cutâneas à quimioterapia, em especial, aqueles para os quais o dermatologista é requisitado a emitir parecer e a comentar sobre a segurança e a viabilidade da readministração de uma droga específica. Os autores descrevem os aspectos associados a esses eventos, fazendo uma análise detalhada de cada um deles.

Events and reactions involving chemotherapy are common in clinical oncology. Chemotherapeutic agents are widely used in therapy. Side effects range from the common to the rare and may be confused with other mucocutaneous manifestations resulting from the oncological treatment. The objective of this paper was to present data on skin reactions to chemotherapy, particularly those cases in which the dermatologist is requested to issue a report and asked to comment on the safety and viability of readministration of a specific drug. The authors describe aspects associated with these events, presenting a detailed analysis of each one of them.