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Ação in vitro e in vivo de fungicida sistêmico e multissítio sobre Phakopsora pachyrhizi
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Kajihara, Luciano Hiroyuki

; Beriam, Luis Otávio Saggion

; Furlan, Silvânia Helena

; Leite, Juliana Aparecida Borelli Pereira

Summa Phytopathologica

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dec 2021, Volume 47 N. 4 Pages 216 - 221

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O trabalho teve por objetivo avaliar a ação do fungicida sítio-específico carbendazim + tebuconazole e do fungicida multissítio clorotalonil, in vitro, sobre a germinação dos uredosporos de Phakopsora pachyrhizi, e in vivo sobre a severidade dos sintomas da doença em folíolos de soja. Os uredosporos de Phakopsora pachyrhizi empregados nos experimentos foram provenientes de Artur Nogueira-SP, coletados a partir de ensaios de campo realizados em três épocas de cultivo, dentro da safra 2016/17. Para cada época de semeadura foram utilizadas três cultivares distintas, obedecendo-se às recomendações segundo o grupo de maturação de cada uma (precoce, médio e tardio), todas suscetíveis à ferrugem asiática, perfazendo um total de 9 cultivares, ou seja, de 9 coletas. Avaliou-se a ação do fungicida multissítio clorotalonil e sítio-específico carbendazim + tebuconazole sobre a germinação in vitro de urediniosporos e a severidade da doença, pelo método de folhas destacadas, in vivo, tratadas preventivamente, antes da inoculação. Determinou-se as concentrações letais CL50 e CL95 pelo método Probit. O fungicida multissítio clorotalonil foi mais fungitóxico in vitro e in vivo que o fungicida específico carbendazim + tebuconazole para os uredosporos de P. pachyrhizi, em todas as coletas referentes, independente das safras e épocas de semeadura. A partir dos testes in vivo e in vitro, há indicação de haver interação significativa dos cultivares com cada fungicida.

ABSTRACT The present study aimed to evaluate the action of both the site-specific fungicide carbendazim + tebuconazole and the multisite fungicide chlorothalonil, in vitro, on the germination of Phakopsora pachyrhizi uredospores and, in vivo, on the severity of symptoms of the disease in soybean leaflets. The uredospores of Phakopsora pachyrhizi used in the experiments were collected from Artur Nogueira, São Paulo State, during field trials carried out in three growing periods within the 2016/17 season. For each sowing period, three different cultivars, susceptible to Asian soybean rust, were used according to the recommendations for their respective maturation group (early, medium and late), totaling 9 cultivars, i.e., 9 samplings. The action of both the multisite fungicide chlorothalonil and the site-specific fungicide carbendazim + tebuconazole was evaluated on the germination of urediniospores, in vitro, and on the disease severity, in vivo, by the method of detached leaves, preventively treated before inoculation. Lethal concentrations LC50 and LC95 were determined by the Probit method. The multisite fungicide chlorothalonil was more fungitoxic in vitro and in vivo than the specific fungicide carbendazim + tebuconazole to P. pachyrhizi uredospores in all samplings, regardless of seasons and sowing times. The in vivo and in vitro tests indicated significant interaction of the cultivars with each fungicide.

https://doi.org/10.1590/0100-5405/251869

Intoxicação experimental por folhas de Crotalaria pallida (mucronata) em ovinos
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Borelli, Vanessa

; Cardoso, Thalita C.

; Biffi, Claudia P.

; Wicpolt, Nathalia

; Ogliari, Daiane

; Savari, Thaisa

; Traverso, Sandra D.

; Gava, Aldo

Pesquisa Veterinária Brasileira

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oct 2016, Volume 36 N. 10 Pages 935 - 938

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Descrevem-se o quadro clínico, patológico e o potencial tóxico das folhas verdes de Crotalaria pallida em ovinos. Para o experimento folhas verdes de C. pallida foram coletadas no município de São João do Sul em Santa Catarina e administradas por via oral para seis ovinos adultos, em doses únicas de 40, 20, 10, 5, 2,5 e 2,5g/kg, e para dois ovinos, em doses diárias de 0,63 e 1,25g/kg durante 30 dias. Reproduziu-se a intoxicação com doses letais a partir de 2,5g/kg. Um dos ovinos que recebeu a dose única de 2,5g/kg e os que receberam as doses diárias, não adoeceram. Todos os ovinos que apresentaram sinais clínicos morreram entre 13 e 22 horas após a administração das folhas. Sinais clínicos leves como fezes pastosas, inquietação e aumento das frequências cardíaca e respiratória tiveram início cerca em 11 horas após a ingestão da planta. Minutos antes da morte observaram-se dispneia intensa com respiração abdominal pronunciada. As principais lesões macroscópicas foram restritas à cavidade torácica, como hidrotórax e edema pulmonar acentuado. O exame microscópico revelou no pulmão, edema acentuado e difuso na superfície pleural, nos espaços interlobulares e ao redor das artérias peribronquiais e peribronquiolares. Edema multifocal foi observado no interior de alvéolos e ao redor de brônquios e bronquíolos, além de leve infiltrado neutrofílico multifocal. O quadro clínico-patológico da intoxicação experimental foi similar ao observado na intoxicação espontânea e caracterizou-se por edema iulmonar acentuado e morte logo após o início dos sinais clínicos.

ABSTRACT: This paper describes the toxic potential of the green leaves of Crotalaria pallida and the clinical signs in experimentally poisoned sheep. Green leaves of C. pallida were collected in São João do Sul, Santa Catarina, and administered orally to six adult sheep, as single dose of 40, 20, 10, 5, 2.5 and 2.5g/kg, and for two sheep at doses of 0.63 and 1.25g/kg during 30 days. The poisoning was reproduced at lethal doses of 2.5g/kg. One of the sheep that received a single dose of 2.5g/kg and another that received daily doses did become ill. All sheep wich developed clinical signs died between 13 and 22 hours after administration of the plant. Slight signs started in about 11 hours after ingestion of the plant, and clinical signs included loose stool, restlessness and increased heart and respiratory rates. A few minutes preceding death, pronounced abdominal breathing and severe dyspnea was observed. The main gross lesions were restricted to the thoracic cavity, as severe pulmonary edema and hydrothorax. Lung microscopy showed marked edema in the diffuse pleural surface, the interlobular spaces and around the peribronchial arteries. Multifocal edema was observed within alveoli and around the bronchi and bronchioles, and mild multifocal infiltration of neutrophils. The clinical and pathological alerations of the experimental poisoning was similar to that observed in the spontaneous poisoning, characterized by marked pulmonary edema and death shortly after the onset of clinical signs.

https://doi.org/10.1590/s0100-736x2016001000003 1388 downloads

Intoxicação espontânea e experimental por frutos de Eugenia uvalha Cambess. (Myrtaceae) em ovinos
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Emmerich, Tiffany

; Borelli, Vanessa

; Cardoso, Thalita C.

; Wisser, Claudia S.

; Wicpolt, Nathalia S.

; Biff, Claudia P.

; Traverso, Sandra D.

; Gava, Aldo

Pesquisa Veterinária Brasileira

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may 2014, Volume 34 N. 5 Pages 438 - 442

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Descrevem-se os dados epidemiológicos, sinais clínicos e lesões de uma enfermidade de ovinos caracterizada por apatia, sialorréia, ranger de dentes, andar em círculos, cegueira, incoordenação motora, opistótono e convulsões, geralmente seguidos de morte. A doença ocorreu nos meses de fevereiro e março de 2009 a 2013, nas regiões Oeste e Planalto do Estado de Santa Catarina e está associada à queda dos frutos da "uvaieira" (Eugenia uvalha Cambess). Nas propriedades onde ocorreram os surtos havia grande quantidade de frutos caídos ao chão e os ovinos os consumiam avidamente. Os que sobreviveram permaneceram com convulsões intermitentes. Não foram observadas alterações macroscópicas ou histológicas. No entanto, foram encontrados frutos inteiros ou fragmentados, misturados ao conteúdo dos pré-estômagos e intestinos. A doença foi reproduzida a partir do fornecimento de frutos maduros de uvaia à três ovinos, nas doses diárias de 45,45g/kg, 68,18g/kg e 82,35g/kg por até seis dias. A principal forma de diagnóstico de intoxicação por "uvaia" é a observação do quadro clínico e epidemiológico, associado à presença de frutos e sementes no trato gastrointestinal.

We describe the epidemiological, clinical signs and lesions of a disease of sheep characterized by apathy, sialorrhoea, teeth grinding, circling, blindness, incoordination, seizures and opisthotonos usually followed by death. The disease occurred in February and March 2009 of 2013, in the West highlands and mountanous regions of Santa Catarina State and was associated with the falling fruits of "uvaieira" (Eugenia uvalha Cambess). In farms where outbreaks occurred had loads of fruit fallen to the ground and the sheep ate them hungrily. Those who survived remained with intermittent seizures. No gross or histological changes were observed. However, the whole fruits or their fragments mixed in the content of the pre-stomachs and bowels were found. The disease was reproduced providing the uvaia ripe fruits to three sheep, in daily doses of 45,45 g/kg, 68,18 g/kg and 82,35 g/kg each, for until six days. The main way to diagnose the uvaia poisoning is the observation of the clinical and epidemiological data, associated with the presence of fruits and seeds in the gastrointestinal tract.

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Avaliação da sensibilidade e especificidade dos exames não invasivos no diagnóstico da estenose de artéria renal
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Borelli, Flavio Antonio de Oliveira

; Pinto, Ibraim M. F.

; Amodeo, Celso

; Smanio, Paola E. P.

; Kambara, Antonio M.

; Petisco, Ana Claudia G.

; Moreira, Samuel M.

; Paiva, Ricardo Calil

; Lopes, Hugo Belotti

; Sousa, Amanda G. M. R.

Arquivos Brasileiros de Cardiologia

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nov 2013, Volume 101 N. 5 Pages 423 - 433

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

FUNDAMENTO: O envelhecimento e a aterosclerose estão relacionados à hipertensão renovascular em indivíduos idosos. Independentemente das comorbidades, a estenose de artéria renal é, por si só, importante causa de morbidade e mortalidade cardiovascular. OBJETIVO: Definir a sensibilidade, a especificidade, o valor preditivo positivo e o valor preditivo negativo dos exames não invasivos utilizados no diagnóstico de estenose da artéria renal. MÉTODOS: Um grupo de 61 pacientes recrutados permitiram a análise de 122 artérias e a definição de sensibilidade, especificidade e da contribuição relativa de cada exame realizado (Doppler, cintilografia e angiotomografia, comparados a arteriografia renal). RESULTADOS: A média das idades foi de 65,43 (desvio padrão: 8,7) anos. Das variáveis relacionadas à população do estudo e comparadas à arteriografia, duas estiveram correlacionadas à estenose da artéria renal, à disfunção renal e aos triglicerídeos. A mediana do ritmo de filtração glomerular foi de 52,8 mL/min/m². O Doppler identificou sensibilidade de 82,90%, especificidade de 70%, valor preditivo positivo de 85% e valor preditivo negativo de 66,70%. Para a tomografia, encontraram-se sensibilidade de 66,70%, especificidade de 80%, valor preditivo positivo de 87,50% e valor preditivo negativo de 55,20%. Esses achados permitiram identificar os exames que melhor detectavam a estenose. CONCLUSÃO: A tomografia e o Doppler mostraram qualidade e grande possibilidade no diagnóstico de estenose da artéria renal, com vantagem para o segundo, pois não há necessidade do uso de meio de contraste na avaliação de uma doença que, frequentemente, ocorre em diabéticos e associa-se à disfunção renal e à disfunção ventricular esquerda grave.

BACKGROUND: Aging and atherosclerosis are related to renovascular hypertension in elderly individuals. Regardless of comorbidities, renal artery stenosis is itself an important cause of cardiovascular morbidity and mortality. OBJECTIVE: To define the sensitivity, specificity, positive predictive value, and negative predictive value of noninvasive imaging tests used in the diagnosis of renal artery stenosis. METHODS: In a group of 61 patients recruited, 122 arteries were analized, thus permitting the definition of sensitivity, specificity, and the relative contribution of each imaging study performed (Doppler, scintigraphy and computed tomographic angiography in comparison to renal arteriography). RESULTS: The mean age was 65.43 years (standard deviation: 8.7). Of the variables related to the study population that were compared to arteriography, two correlated with renal artery stenosis, renal dysfunction and triglycerides. The median glomerular filtration rate was 52.8 mL/min/m². Doppler showed sensitivity of 82.90%, specificity of 70%, a positive predictive value of 85% and negative predictive value of 66.70%. For tomography, sensitivity was 66.70%, specificity 80%, positive predictive value 87.50% and negative predictive value 55.20%. With these findings, we could identify the imaging tests that best detected stenosis. CONCLUSION: Tomography and Doppler showed good quality and efficacy in the diagnosis of renal artery stenosis, with Doppler having the advantage of not requiring the use of contrast medium for the assessment of a disease that is common in diabetics and is associated with renal dysfunction and severe left ventricular dysfunction.

https://doi.org/10.5935/abc.20130191 4466 downloads

I posicionamento brasileiro em hipertensão arterial e diabetes mellitus
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Alessi, Alexandre

; Bonfim, Alexandre Vidal

; Brandão, Andrea A.

; Feitosa, Audes

; Amodeo, Celso

; Alves, Claudia Rodrigues

; Brasil, David P.

; Souza, Dilma do SM

; Barbosa, Eduardo

; Consolim-Colombo, Fernanda Marciano

; Borelli, Flávio

; Fonseca, Francisco Helfenstein

; Lopes, Heno F.

; Chaves, Hilton

; Bortolotto, Luis Aparecido

; Martin, Luis C.

; Scala, Luiz Cesar Nazário

; Mota-Gomes, Marco Antonio

; Malachias, Marcus Vinícius Bolívar

; Izar, Maria Cristina

; Fonseca, Marília Izar Helfenstein

; Neves, Mário Fritsch Toros

; Morais, Nelson Siqueira

; Passarelli Jr, Oswaldo

; Jardim, Paulo Cesar Veiga

; Toscano, Paulo Roberto

; Miranda, Roberto Dischinger

; Franco, Roberto

; Betti, Roberto Tadeu Barcellos

; Pedrosa, Rodrigo Pinto

; Povoa, Rui

; Carneiro, Sérgio Baiocchi

; Jardim, Thiago

; Barroso, Weimar Kunz Sebba

Arquivos Brasileiros de Cardiologia

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jun 2013, Volume 100 N. 6 Pages 491 - 501

Abstract: Pt | Text: Pt | PDF: Pt

https://doi.org/10.5935/abc.20130123 8219 downloads Cited 2 times in SciELO

Análise comparativa entre a vascularização arterial ventricular e do nó sinoatrial em corações de cães
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Biasi, Caio

; Borelli, Vicente

; Prazeres, Rodrigo F.

; Favaron, Phelipe O.

; Pavanelo Junior, Valdir

; Aloia, Thiago P.A.

; Bombonato, Pedro P.

Pesquisa Veterinária Brasileira

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jan 2013, Volume 33 N. 1 Pages 111 - 114

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Utilizamos nesta pesquisa 40 corações de cães adultos, machos e fêmeas, de idades variadas, que não portavam nenhuma afecção cardíaca. Os corações tiveram as artérias coronárias injetadas, separadamente, com Neoprene Látex 450, corado com pigmento vermelho, e posteriormente dissecados. Em todas estas preparações verificamos que na vascularização dos ventrículos predominava a artéria coronária esquerda que fornecia os ramos interventriculares paraconal e subsinuoso. Já, a região ocupada pelo nó sinoatrial ficava mais frequentemente (17 vezes, 42,5%) na dependência do ramo proximal atrial esquerdo ou de colateral deste vaso, oriundo do ramo circunflexo esquerdo, ou deste vaso associado ao ramo distal atrial direito (8 vezes, 20%), procedente do ramo circunflexo direito. Com menor frequência (14 vezes, 30%), a área tomada pelo nó sinoatrial, encontramos apenas colaterais do ramo circunflexo direito, mais exatamente somente o ramo distal atrial direito (10 vezes, 25%), apenas o ramo proximal atrial direito (3 vezes, 7,5%) ou ainda exclusivamente o ramo intermédio atrial direito (1 vez, 2,5%). Em um único caso (1 vez, 2,5%) no território do nó sinoatrial observamos apenas colateral do ramo circunflexo esquerdo, isto é o ramo distal atrial esquerdo. A análise destes resultados permite concluir, que nesta espécie não existe qualquer tipo de relação entre o tipo de vascularização dos ventrículos e a irrigação do nó sinoatrial. Sendo assim, considerar os ramos ventriculares isoladamente não é suficiente para um entendimento clínico-cirúrgico aplicado, uma vez que os ramos atriais apresentam uma importante contribuição para a vascularização do nó sinoatrial.

We analyzed 40 hearts of adult dogs, males and females of different ages, without cardiac disease. The hearts had the coronary arteries inject using Neoprene Latex 450, colored with red pigment, and which was then dissected. In the ventricular vascularization the left coronary artery was predominant and gave rise to the paraconal interventricular and subsinuous branches. The region occupied by the sinoatrial node was most frequently (17 times, 42.5%) in dependence of the left atrial proximal branch or in the collateral branch of that vessel, coming from the left circumflex branch, or this vessel was associated with the right atrial distal branch (8 times, 20%). With less frequency (14 times, 30%), in the area occupied by the sinoatrial node we found only the collaterals of the right circumflex branch, only the right atrial distal branch (10 times, 25%), only the right atrial proximal branch (3 times, 7.5%) or exclusively the right atrial intermediary branch (once, 2.5%). In just one case (once, 2.5%) in the area of the sinoatrial node we observed only the collateral of the left circumflex branch, i.e. the left atrial distal branch. According to our results we concluded that in this specie there is no relationship between the type of ventricular vascularization and irrigation of the sinoatrial node. In doing so, to consider just the ventricular branches is not sufficient for a clinical and surgical applied understanding, since the atrial branches make an important contribution to the sinoatrial node vascularization.

3413 downloads Cited 2 times in SciELO

Impairment of the hematological response and interleukin-1β production in protein-energy malnourished mice after endotoxemia with lipopolysaccharide
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Fock, R.A.

; Vinolo, M.A.R.

; Blatt, S.L.

; Borelli, P.

Brazilian Journal of Medical and Biological Research

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dec 2012, Volume 45 N. 12 Pages 1163 - 1171

Abstract: En | Text: En | PDF: En

The objectives of this study were to determine if protein-energy malnutrition (PEM) could affect the hematologic response to lipopolysaccharide (LPS), the interleukin-1β (IL-1β) production, leukocyte migration, and blood leukocyte expression of CD11a/CD18. Two-month-old male Swiss mice were submitted to PEM (N = 30) with a low-protein diet (14 days) containing 4% protein, compared to 20% protein in the control group (N = 30). The total cellularity of blood, bone marrow, spleen, and bronchoalveolar lavage evaluated after the LPS stimulus indicated reduced number of total cells in all compartments studied and different kinetics of migration in malnourished animals. The in vitro migration assay showed reduced capacity of migration after the LPS stimulus in malnourished animals (45.7 ± 17.2 x 10(4) cells/mL) compared to control (69.6 ± 7.1 x 10(4) cells/mL, P ≤ 0.05), but there was no difference in CD11a/CD18 expression on the surface of blood leukocytes. In addition, the production of IL-1β in vivo after the LPS stimulus (180.7 pg·h-1·mL-1), and in vitro by bone marrow and spleen cells (41.6 ± 15.0 and 8.3 ± 4.0 pg/mL) was significantly lower in malnourished animals compared to control (591.1 pg·h-1·mL-1, 67.0 ± 23.0 and 17.5 ± 8.0 pg/mL, respectively, P ≤ 0.05). The reduced expression of IL-1β, together with the lower number of leukocytes in the central and peripheral compartments, different leukocyte kinetics, and reduced leukocyte migration capacity are factors that interfere with the capacity to mount an adequate immune response, being partly responsible for the immunodeficiency observed in PEM.

1578 downloads

Variabilidad del gen RYR1 y efecto en el pH de la carne en cerdos híbridos argentinos
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Marini, S.J.

; Vanzetti, L.S.

; Borelli, V.S.

; Villareal, A.O.

; Denegri, G.D.

; Cottura, G.A.

; Panichelli, D.

; Silva, P.

; Campagna, D.

; Spiner, N.

; Brunori, J.C.

; Franco, R.

InVet

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jun 2012, Volume 14 N. 1 Pages 19 - 23

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The RYR1 gene is the principal cause of the porcine stress syndrome (PSS) and the presence of pale, soft and exudative meat (PSE) in swine. In Argentina there are no reports about the variability of RYR1 gene in hybrid swine and its effect on the quality of carcass. By PCR-HRM we analyzed 403 hybrids swines from Argentina and 65.0% were homozygous NN, 30.8% heterozygous Nn and 4,2% homozygous nn. Additionally, a significant effect (p <0.0001) of the RYR1 gene was detected on the pH of carcass at 45 minutes after slaughter. Normal genotypes NN showed the highest pH values at 45 minutes after slaughter (6.27), heterozygous Nn showed intermediate pH values (5.94) and homozygous susceptible nn showed the lowest pH values (5.64). Argentina present high incidence of Nn and nn genotypes for the RYR1 gene in relation to Brazil, the principal pig producer of Latin America. Additionally the RYR1 gene affects the pH of the meat, one of the principal parameters in the determination of the PSE meat.

El gen RYR1 es la principal causa del síndrome de estrés porcino (PSS) y la presencia de carne pálida, blanda y exudativa (PSE) en los cerdos. En Argentina no existen informes sobre la variabilidad de dicho gen en cerdos híbridos y su efecto sobre la calidad de la canal. Por PCR-HRM se analizaron 403 cerdos híbridos de Argentina y el 65,0% eran homocigotos NN, 30.8% heterocigotos Nn y el 4,2% homocigotos nn. Además, se detectó un efecto significativo (p <0,0001) del gen RYR1 en el pH de la canal a los 45 minutos post faena. Los genotipos NN mostraron los mayores valores de pH a los 45 minutos después del sacrificio (6.27), los heterocigotos Nn mostraron valores intermedios de pH (5,94) y los homocigotos nn presentaron los valores más bajos de pH (5,64). Argentina presenta alta incidencia de los genotipos Nn y nn del gen RYR1 en relación a Brasil, el principal productor de cerdos de América Latina. Además, el gen RYR1 afecta al pH de la carne, uno de los principales parámetros para la determinación de carnes PSE.

250 downloads

Análise comparativa entre a vascularização ventricular e do nó sinoatrial em gatos
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Biasi, Caio

; Borelli, Vicente

; Benedicto, Hildebrando G.

; Pereira, Miler R.

; Favaron, Phelipe O.

; Bombonato, Pedro P.

Pesquisa Veterinária Brasileira

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jan 2012, Volume 32 N. 1 Pages 78 - 82

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A possível existência de interdependência na nutrição de territórios atriais e ventriculares tem sido objeto de preocupação por partes dos cardiologistas, especialmente no que tange a vascularização do nó sinoatrial e sua dependência apenas de uma artéria coronária ou de ambas e de sua relação com o predomínio destes vasos na vascularização ventricular. Assim, este estudo objetiva avaliar a relação da irrigação do nó sinoatrial e a origem e a predominância das artérias coronárias na vascularização dos ventrículos, para tanto utilizou-se 30 corações de gatos sem raça definida adultos, machos e fêmeas, sem sinais de afecção cardíaca. Os corações foram injetados pela aorta torácica com Neoprene Latex 450, corados com pigmento vermelho e dissecados posteriormente. Verificou-se que quando ocorria predomínio da vascularização ventricular do tipo esquerda (63,34%) a irrigação do nó sinoatrial ficou predominantemente na dependência do ramo proximal atrial direito (78,9%) ou com menor freqüência pelo ramo proximal atrial esquerdo (21,1%). Na vascularização ventricular do tipo equilibrada (33,34%), a irrigação do sinoatrial ficou na dependência mais freqüentemente do ramo proximal atrial direito (80%), ou com menor freqüência a nutrição do nó se deu pelo ramo proximal atrial esquerdo (20%). Em um caso isolado, ocorreu a vascularização ventricular do tipo direita (3,34%), a nutrição do sinoatrial, ficou na dependência exclusiva do ramo intermédio atrial direito. Estes resultados indicam que nesta espécie não existe relação entre a irrigação do nó sinoatrial e o tipo de vascularização ventricular, independentemente do sexo.

The possible existence of interdependence in the blood nutrition of both atrial and ventricular territories has been a subject of concern to cardiologists, mainly related to vascularization of the sinoatrial node and its dependence on just one coronary artery or both, and its relation with the predominance of these vessels in the ventricular vascularization. Therefore, this research aimed evaluated the relation of blood irrigation of the sinoatrial node in relation to the coronary artery predominance in the ventricle vascularization. In doing so, we analyzed 30 hearts of cats without pedigree, males and females, adults of several ages. They were not carrying any heart problems. The hearts were injected by the thoracic aorta with Neoprene Latex 450, stained with red pigment, and then they were dissected. It was found that when there was a prevalence of ventricular vascularization of the left type (63.34%) the sinoatrial node irrigation was predominantly in the dependency of the Ramus proximalis atrii dextri (78.9%) or with less frequency by Ramus proximalis atrii sinister (21.1%). In the ventricular vascularization of the balanced type (33.34%), the pacemaker irrigation was in dependence more often of Ramus proximalis atrii dextri (80%) or with less frequency the nutrition of the sinoatrial node occurred by Ramus proximalis atril sinister (20%). In a single-case, we observed the ventricular vascularization of the right type (3.34%), the pacemaker nutrition was in an exclusive dependence of the Ramus intermedius atril dextri. These results suggest in this species there is no relationship between both the sinoatrial node irrigation and the type of ventricular vascularization, regardless of gender.

3085 downloads Cited 2 times in SciELO

10.

Morfologia do funículo espermático em caprinos da raça Bhuj Brasileira
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Costa, Luis A.O. P.

; Abidu-Figueiredo, Marcelo

; Machado-Santos, Clarice

; Borelli, Vicente

; Pereira, João G. L.

Pesquisa Veterinária Brasileira

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aug 2011, Volume 31 N. 8 Pages 711 - 717

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Estudamos em 40 caprinos adultos da raça Bhuj Brasileira os aspectos histológicos do funículo espermático. Observamos que este se acha envolvido por uma cápsula de tecido conjuntivo fibroelástico denso, de espessura variável, pregueada em alguns pontos, e revestida por mesotélio que circunda todo o conjunto vásculo-nervoso, e projeta-se para formar o mesoducto deferente. Em posição subcapsular, verifica-se uma camada de tecido conjuntivo fibroelástico frouxo, de espessura variável, que circunda parcialmente o funículo espermático, isolando nas regiões deferencial e abdeferencial, conjuntos vásculo-nervosos, responsáveis pela nutrição do epidídimo. Na região do mesoducto deferente, o tecido subcapsular acompanhado de tecido adiposo constitui a camada interna deste meso, formando a sua adventícia e abrigando vasos e nervos deferenciais. Na região abdeferencial, pequenos acúmulos de tecido adiposo são vistos de permeio aos vasos e nervos desta região. Entre as artérias, veias e nervos testiculares, bem como entre os vasos das regiões deferencial e abdeferencial, observa-se o tecido conjuntivo denso, intervascular, rico em fibras elásticas, que constitui as adventícias contínuas destes vasos. O arranjo vascular mostra que o segmento da artéria testicular, contido no funículo espermático, apresenta trajeto sinuoso. Estando envolvido pelo plexo venoso pampiniforme, formado por veias testiculares desprovidas de válvulas de calibres variados, apresentando amplas comunicações entre si. As veias responsáveis pela drenagem do epidídimo e ducto deferente estão localizadas em posição subcapsular deferencial e abdeferencial e mostram-se providas de válvulas. O trato das artérias testiculares no funículo espermático apresenta como média e desvio padrão 134,6±38,1cm à direita, e 137,0±33,9cm à esquerda, não existindo diferenças estatisticamente significantes ao nível de 5%, quando comparamos a média do segmento da artéria testicular contida no funículo espermático direito em relação ao esquerdo.

Histological aspects of the spermatic cord were studied in 40 Brazilian Bhuj goats. It was seen that the spermatic cord is involved by a thin capsule of compact connective tissue recovered by mesothelium which forms an expansion, the mesodeferens. Under this capsule is a subcapsular layer of variable thickness, formed by loose elastic fiber connective tissue that mixes with deep layer of the mesodeferens and with the adventitia of ductus deferens. Between the testicular artery and testicular veins exists an intervascular compact elastic fiber connective tissue in continuity with the adventitia of spermatic cord vessels. In the abdeferential region of the spermatic cord exists a neurovascular contingent, the vessels of which generally have inferior calibers and similar constitution and arrangement as the testicular artery and veins; the described contingent is related to nutrition of the initial portion of ductus deferens and part of the epididymis head and body. The vascular arrangement shows that the segment of the testicular artery in the spermatic cord has a sinuous disposition and is totally involved by the venous plexus formed by testicular vein valves with irregular outlines, diverse calibers and large connections. The length of the intra spermatic cord segment of the testicular artery has a medium and standard deviation of 134.6±38.1cm on the right and 137±33.9cm on the left. There were no statistical differences between the right and left medium ranges.

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11.

Protein-energy malnutrition halts hemopoietic progenitor cells in the G0/G1 cell cycle stage, thereby altering cell production rates
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Borelli, P.

; Barros, F.E.V.

; Nakajima, K.

; Blatt, S.L.

; Beutler, B.

; Pereira, J.

; Tsujita, M.

; Favero, G.M.

; Fock, R.A.

Brazilian Journal of Medical and Biological Research

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jun 2009, Volume 42 N. 6 Pages 523 - 530

Abstract: En | Text: En | PDF: En

Protein energy malnutrition (PEM) is a syndrome that often results in immunodeficiency coupled with pancytopenia. Hemopoietic tissue requires a high nutrient supply and the proliferation, differentiation and maturation of cells occur in a constant and balanced manner, sensitive to the demands of specific cell lineages and dependent on the stem cell population. In the present study, we evaluated the effect of PEM on some aspects of hemopoiesis, analyzing the cell cycle of bone marrow cells and the percentage of progenitor cells in the bone marrow. Two-month-old male Swiss mice (N = 7-9 per group) were submitted to PEM with a low-protein diet (4%) or were fed a control diet (20% protein) ad libitum. When the experimental group had lost about 20% of their original body weight after 14 days, we collected blood and bone marrow cells to determine the percentage of progenitor cells and the number of cells in each phase of the cell cycle. Animals of both groups were stimulated with 5-fluorouracil. Blood analysis, bone marrow cell composition and cell cycle evaluation was performed after 10 days. Malnourished animals presented anemia, reticulocytopenia and leukopenia. Their bone marrow was hypocellular and depleted of progenitor cells. Malnourished animals also presented more cells than normal in phases G0 and G1 of the cell cycle. Thus, we conclude that PEM leads to the depletion of progenitor hemopoietic populations and changes in cellular development. We suggest that these changes are some of the primary causes of pancytopenia in cases of PEM.

4137 downloads Cited 2 times in SciELO

12.

Septo-optic dysplasia: case report
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Pierre Filho, Paulo de Tarso P.

; Bovo, Lucas Borelli

; Marcondes, Ana Maria

Arquivos Brasileiros de Oftalmologia

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aug 2004, Volume 67 N. 4 Pages 653 - 655

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A tríade clínica da displasia septo-óptica (DSO) compreende a ausência de septo pelúcido, displasia congênita do nervo óptico e múltiplos defeitos endócrinos. Uma forma incompleta de DSO tem sido reconhecida com dois dos três elementos. Os autores relatam um caso de displasia septo-óptica na forma incompleta em um garoto de 9 anos com baixa visão e nistagmo presentes desde o nascimento. O exame oftalmoscópico revelou papila pequena com imagem em duplo contorno bilateralmente. Ressonância magnética mostrou hipoplasia dos nervos ópticos, quiasma e ausência do septo pelúcido.

The clinical triad of septo-optic dysplasia (SOD) comprises the absence of the septum pellucidum, congenital optic nerve dysplasia, and multiple endocrine disorders. When any two of these factors are present, the condition is defined as an incomplete form of SOD. The authors report the case of an incomplete form of SOD in a 9-year-old boy with low vision and nystagmus present from birth. The bilateral ophthalmoscopic examination revealed small papillae with double contour images. Magnetic resonance imaging showed hypoplasia of the optic nerve bilaterally, chiasm and absence of the septum pellucidum.

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13.

Alterations in proteins of bone marrow extracellular matrix in undernourished mice
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Vituri, C.L.

; Alvarez-Silva, M.

; Trentin, A.G.

; Borelli, P.

Brazilian Journal of Medical and Biological Research

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aug 2000, Volume 33 N. 8 Pages 889 - 895

Abstract: En | Text: En | PDF: En

The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4% casein as compared to 20% casein in the control diet. When the experimental group had attained a 20% loss of their original body weight, we extracted the ECM proteins from bone marrow with PBS buffer, and analyzed ECM samples by SDS-PAGE (7.5%) and ECL Western blotting. Quantitative differences were observed between control and experimental groups. Bone marrow ECM from undernourished mice had greater amounts of extractable fibronectin (1.6-fold increase) and laminin (4.8-fold increase) when compared to the control group. These results suggest an association between fluctuations in the composition of the hematopoietic microenvironment and altered hematopoiesis observed in undernourished mice.

2862 downloads Cited 6 times in SciELO

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