Abstract Background Areas simultaneously infested with both glyphosate-resistant sourgrass (Digitaria insularis L.) and fleabane (Conyza spp.) are usual in Brazil. However, there are no effective management strategies to control both species concurrently. Objective The aim was to evaluate the interaction and interval of application of haloxyfop-P-methyl and cloransulam-methyl to effective control sourgrass (from 3- to 4-tillers to flowering stages) and fleabane (12- to 15-cm at the vegetative stage). Methods The experiment was conducted twice in a randomized complete blocks design with four biological replicates. The treatments were composed of sequential applications of haloxyfop-P-methyl (firstly applied) and cloransulam-methyl (secondly applied) and vice-versa. After the first application, the second one was applied in intervals of 3, 6 and 12 days. Haloxyfop-P-methyl and cloransulam-methyl were also applied in sequential (without interval), stand-alone applications of both herbicides and an untreated check. Results All treatments effectively controlled all fleabane plants. Haloxyfop-P-methyl applied ≥ 6 days before cloransulam-methyl controlled 100% of 3- to 4-tillers sourgrass and 60% of flowering sourgrass. Conclusions Therefore, haloxyfop-P-methyl may be applied ≥ 6 days before cloransulam-methyl in a sequential application structure to effectively control fleabane and sourgrass (3- to 4-tillers stage), when present concurrently.

RESUMO O longo período residual dos herbicidas pré-emergentes pode interferir em culturas sucessoras, ocasionando o fenômeno denominado de efeito residual (carryover). O trabalho teve como objetivo avaliar o efeito residual dos herbicidas imazapir + imazapic na cultura de algodão em sucessão à soja. O experimento foi conduzido entre setembro de 2016 a março de 2017, no delineamento em blocos, com quatro repetições, sendo os tratamentos quatro doses de imazapir + imazapic, 75, 150, 225 e 300 g i.a. ha-1 e a testemunha. As variáveis analisadas foram fluorescência da clorofila a, altura da planta, comprimento e volume da raiz, massa seca da parte aérea e massa seca da raiz. Os dados gerados foram submetidos à análise da variância e ao teste de Tukey, a p ≤ 0,05 de probabilidade. A diminuição nos índices de desempenho fotossintético no algodão nos tratamentos com doses de 150, 225 e 300 g i.a. ha-1 aos 15 dias após emergência (DAE), e com dose de 75 g i.a. ha-1 aos 60 DAE, indica o efeito fotoinibitório destes herbicidas sobre as plantas, entretanto, as variáveis altura da planta, comprimento, volume e massa seca da raiz não foram afetados nos tratamentos testados. Portanto, a persistência do herbicida no solo não foi suficiente para caracterizar o efeito residual (carryover) em algodão 135 dias após a aplicação (DAA).

ABSTRACT The long residual period of pre-emergent herbicides can interfere with successor crops, causing a phenomenon named carryover. This study evaluated the residual effect of imazapyr + imazapic herbicides on cotton crop grown in succession to soybean. The experiment was conducted between September 2016 and March 2017, in a randomized block design with 4 replicates, where the treatments were four doses of imazapyr + imazapic, 75, 150, 225 and 300 g a.i. ha-1, and the control. The analyzed variables were chlorophyll a fluorescence, shoot height, root length and volume, dry shoot mass and root dry mass. The data were submitted to analysis of variance and Tukey’s test, at p ≤ 0.05. The reduction in photosynthetic performance indexes for treatments of 150, 225 and 300 g a.i. ha-1 at 15 days after emergence (DAE) and of 75 g a.i ha-1 at 60 DAE indicated a photoinhibitory effect of these herbicides on plants. However, shoot height, length, volume and root dry mass were not affected in the treatments tested. Therefore, the persistence of the herbicide in the soil was not enough to characterize a carryover effect in cotton 135 days after application (DAA).

ABSTRACT: In this work, we compared nitrate-supplied plants (non-nodulated) with non-nitrate-supplied plants (nodulated) under oxygen privation of root system (hypoxia) and re-oxygenation (post-hypoxia; recovery) in order to verify whether N sources affect the antioxidant system during oxidative stress caused by hypoxia and post-hypoxia conditions. Antioxidant enzymatic activities, ascorbate redox state, and reactive oxygen species (ROS) levels were analyzed in roots and leaves of two soybean genotypes, Fundacep 53 RR and BRS Macota at reproductive stage R2, during hypoxia and post-hypoxia in an experiment carried out in a hydroponic system. The antioxidant system was strongly induced in roots of nitrate-supplied plants of both genotypes, with high activity of superoxide dismutase, ascorbate peroxidase, catalase, glutathione reductase and guayacol peroxidase. It also increased reduced ascorbate and ascorbate redox state and decreased ROS production under hypoxia and recovery, while in leaves of nodulated and non-nodulated plants, a slight increase on antioxidant system was observed. Nitrate may benefit soybean plants under hypoxic conditions and subsequent re-oxygenation by inducing the antioxidant system mainly in roots to cope with ROS production and reduce oxidative damage.

Waterlogging blocks the oxygen supply to the root system which inhibits respiration, and greatly reduces the energy status of cells that affect important metabolic processes. This study evaluated fermentative metabolism and carbohydrate contents in the root system of two soybean (Glycine max L. Merril) genotypes under hypoxic and post-hypoxic conditions. Nodulated plants (genotypes Fundacep 53 RR and BRS Macota) were grown in vermiculite and transferred to a hydroponic system at the reproductive stage. The root system was submitted to hypoxia by flowing N2 (nitrogen) gas in a solution for 24 and 72 h. For recovery, plants returned to normoxia condition by transfer to vermiculite for 24 and 72 h. Fermentative enzyme activity, levels of anaerobic metabolites and carbohydrate content were all quantified in roots and nodules. The activity of alcohol dehydrogenase, pyruvate decarboxylase and lactate dehydrogenase enzymes, as well as the content of ethanol and lactate, increased with hypoxia in roots and nodules, and subsequently returned to pre-hypoxic levels in the recovery phase in both genotypes. Pyruvate content increased in nodules and decreased in roots. Sugar and sucrose levels increased in roots and decreased in nodules under hypoxia in both genotypes. Fundacep RR 53 was more responsive to the metabolic effects caused by hypoxia and post-hypoxia than BRS Macota, and it is likely that these characteristics contribute positively to improving adaptation to oxygen deficiency.

O objetivo deste trabalho foi avaliar a atividade de enzimas fermentativas em raízes e nódulos de soja (Glycine max) sob condições de hipóxia e pós-hipóxia. Os genótipos Fundacep 53 RR e BRS Macota foram cultivados em vasos de 3L contendo vermiculita como substrato em associação com Bradyrhizobium elkanii em casa de vegetação. No estádio R2, o sistema radicular das plantas foi submetido à hipóxia pelo encaixe de um segundo vaso sem perfurações e inundado com solução nutritiva diluída a 1/3 da concentração original, por 8 dias. Posteriormente, foram retirados os vasos sem perfurações para avaliação da recuperação. O material vegetal foi coletado no 2º, 4º, 6º e 8º dias após a inundação ou recuperação, juntamente com os controles para a dosagem da atividade das enzimas álcool desidrogenase (ADH), piruvato descarboxilase (PDC) e lactato desidrogenase (LDH) em raízes e nódulos. Os dados foram submetidos à ANOVA e comparados pelo teste de Tukey a 5% de probabilidade. As atividades da ADH, PDC e LDH foram fortemente estimuladas com a inundação, especialmente em nódulos, e diminuíram com a recuperação, em ambos os genótipos. Fundacep 53 RR respondeu de forma mais efetiva aos efeitos dos tratamentos.

This study aimed to evaluate the activity of fermentative enzymes in soybean (Glycine max) roots and nodules under hypoxia and post-hypoxia conditions. The soybean genotypes Fundacep 53 RR and BRS Macota associated with Bradyrhizobium elkanii were grown in 3L pots containing vermiculite as substrate under greenhouse conditions. Root system of plants at the R2 stage was subjected to hypoxia by blocking drainage of the pot, by fitting a second one without holes and flooded with nutrient solution diluted one-third of the original. The flooding of the plants was carried out for eight days and after, the pot without drilling was removed for recovery assessment. Non-waterlogged plants were kept as control. Activities of the fermentation enzymes alcohol dehydrogenase (ADH), pyruvate decarboxylase (PDC) and lactate dehydrogenase (LDH) were quantified in roots and nodules collected at 2, 4, 6 and 8 days after flooding or recovery. Data were analyzed by ANOVA and compared by the Tukey test (P ≤ 0,05). Activities of ADH, PDC and LDH were hardly stimulated during flooding, especially in nodules, in both genotypes and decreased with recovery. Fundacep 53 RR responded more effectively to treatment effects.

Este trabalho objetivou avaliar os efeitos alelopáticos de folhas de pariparoba sobre a germinação e o crescimento inicial do rabanete (Raphanus sativus L.). Foram utilizados extratos de folhas maduras frescas nas concentrações 2, 4 e 8% (p/v), cujo pH e potencial osmótico foram aferidos. No bioensaio de germinação foram empregadas cinco repetições de 25 sementes distribuídas em placas de petri forradas com papel Germitest, umedecido com 7 mL de extrato ou água e mantidos a 25ºC em B.O.D. por cinco dias. A porcentagem de germinação (PG), velocidade de germinação (VG), índice de velocidade de germinação (IVG) e índice de efeito alelopático (RI) foram determinados. No bioensaio de crescimento inicial, sementes germinadas até atingirem 2 mm de protusão radicular foram transferidas para caixas Gerbox contendo papel Germitest e 15 mL de extrato ou água, permanecendo sete dias a 25ºC em B.O.D. para a avaliação do comprimento da radícula e do hipocótilo, da massa fresca e seca, do conteúdo de água e do teor de clorofila (a, b e total). Todos os parâmetros de germinação (PG, VG, IVG e RI) foram afetados negativamente pelos extratos de P. mikanianum. O crescimento inicial foi influenciado pelos extratos, resultando em redução do comprimento da radícula e aumento do hipocótilo. Além disso, os extratos causaram redução da biomassa fresca e do conteúdo de água a 8% e incrementos dos teores de clorofila b e total a 2 e 4%. Assim, a espécie Piper mikanianum exerce efeito alelopático sobre o rabanete.

This study evaluated the allelopathic effects of an extract from P. mikanianum leaves on the germination and early growth of radish (Raphanus sativus L.). Leaf extracts were prepared at concentrations of 2, 4 and 8%. The pH and osmotic potential of the extracts were determined. Germination bioassay consisted of five replicates of 25 radish seeds distributed in Petri dishes with germitest paper and 7 mL of extract or water, and were kept at 25ºC in B.O.D. for five days. Germination percentage (PG), germination speed (VG), germination speed index (IVG) and index of allelopathic effect (RI) were determined. For bioassay initial growth, seeds were germinated until the protrusion of the radicle was 2 mm and transferred to Gerbox boxes containing germitest paper and 15 mL of extract or water, which were kept for seven days at 25ºC in B.O.D. to evaluate the length of the radicle and hypocotyl, fresh and dry mass, water content and chlorophyll content (a, b and total). Leaf extracts from P. mikanianum negatively affected all parameters examined for germination (PG, VG, IVG and RI). The extracts affected the initial growth, causing reduction of the radicle length and stimulating the growth of the hypocotyl. In addition, the extracts at 8% concentration reduced fresh weight and water content. Extracts at concentrations of 2 and 4% increased the content of chlorophyll b and total chlorophyll. Thus, Piper mikanianum exerted an allelopathic effect on radish.

Neste trabalho, objetivou-se avaliar o efeito alelopático de folhas secas e pseudofrutos de Hovenia dulcis Thunb. sobre sementes de alface. Para isso, prepararam-se extratos aquosos de folhas e pseudofrutos nas concentrações 1, 2 e 4% (m/v). Realizou-se quatro repetições de 25 sementes de alface, distribuídas em placas de Petri forradas com papel Germitest e umedecidas com os extratos, mantidas em câmara de germinação à temperatura de 25°C, fotoperíodo constante e presença de luz por cinco dias, sendo o controle água destilada. Calculou-se a porcentagem, a velocidade, o índice de velocidade de germinação, o comprimento radicular e da parte aérea das plântulas, bem como o efeito das sementes na presença de diferentes soluções de pH. Observou-se que as plântulas foram significativamente afetadas na presença de extratos aquosos de folhas secas e pseudofrutos nas concentrações 2 e 4%. Os efeitos foram desvinculados do pH e potencial osmótico, indicando, portanto, interferência alelopática dos extratos obtidos de Hovenia dulcis.

This paper aims to evaluate the allelopathic effect of dry leaves and pseudofruits of Hovenia dulcis Thunb. on lettuce seeds. Therefore, aqueous extracts of leaves and pseudofruits were prepared in 1%, 2% and 4% (m/v) concentrations. Four replicates were done of 25 lettuce seeds distributed in Petri dishes lined with Germitest paper and humidified with the extracts, maintained in germination chamber at 25 °C, constant photoperiod and light presence for five days, the control being distilled water. The percentage, speed, germination rate index, root length and aerial-part length of the seedlings were calculated, as well as the seeds effect in the presence of different pH solutions. It was observed that the seedlings were significantly affected in the presence of aqueous extracts of dry leaves and pseudofruits at 2% and 4% concentrations. The effects were not linked to pH and osmotic potential, indicating, therefore, allelopathic interference of Hovenia dulcis extracts.

As plantas produzem substâncias por meio do metabolismo secundário. Quando liberadas no meio ambiente são capazes de interferir na germinação e no desenvolvimento de outras plantas. Objetivou-se, nesta pesquisa investigar os efeitos alelopáticos de umbu (Phytolacca dioica L.) sobre a germinação e o crescimento inicial de alface e picão-preto, em laboratório, utilizando-se extratos aquosos de frutos de umbu nas concentrações 0, 1, 2, 4 e 8%; foi realizada análise fitoquímica dos frutos e testes de pH e potencial osmótico dos extratos de frutos. Analisaram-se, para a germinação os parâmetros: porcentagem de germinação (PG), velocidade de germinação (VG) e índice de velocidade de germinação (IVG); para o crescimento inicial analisaram-se os parâmetros: comprimento (radicular e da parte aérea) e biomassa (fresca e seca) das plântulas de alface e picão-preto. Os dados foram submetidos ao teste de Tukey, a 1% de probabilidade. Os parâmetros PG, VG e IVG foram alterados significativamente, proporcionais ao aumento da concentração dos extratos de frutos do umbu. O comprimento (radicular e da parte aérea) e a biomassa (fresca e seca) das plântulas de alface e picão-preto foram reduzidos significativamente com o aumento da concentração dos extratos aquosos de frutos de umbu. O pH e potencial osmótico dos extratos mantiveram-se dentro de padrões adequados e a análise fitoquímica revelou a presença de flavonoides. Pelos resultados os frutos de umbu apresentaram-se com efeito alelopático sobre a alface e o picão-preto.

Plants produce substances through secondary metabolism. When these chemicals are released into the environment they can interfere in the germination and development of other plants. The objective of this research was to investigate the effects of allelopathic Phytolacca dioica L. on the germination and early growth of lettuce and beggartick, under laboratory conditions, using aqueous extracts of fruits of Phytolacca dioica at concentrations of 0, 1, 2, 4 and 8%. Phytochemical analysis of the fruit was performed; and pH and osmotic potential of extracts of fruit were tested. We analyzed the following germination parameters: percentage of germination (PG), speed of germination (VG) and speed of germination index (IVG), for the initial growth parameters examined are: length (root and shoot) and biomass (fresh and dry) of seedlings of lettuce and beggartick. The data were submitted to Tukey's test at 1% of probability. Parameters PG, VG and IVG were significantly affected, with increase in concentration of the extracts of fruits of Phytolacca dioica. The length (root and shoot) and biomass (fresh and dry) of seedlings of lettuce and beggartick were reduced significantly with increasing concentration of the aqueous extracts of fruits of Phytolacca dioica. The pH and osmotic potential of the extracts were maintained within appropriate standards and phytochemical analysis revealed the presence of flavonoids. The results showed that the fruits of Phytolacca dioica presented allelopathic effect on the lettuce and beggartick.