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au:Barreto, Daniel L.

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Anti-Leishmania amazonensis activity of the marine sponge Dercitus (Stoeba) latex (Porifera) from São Pedro and São Paulo Archipelago, Pernambuco, Brazil
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BARRETO, ANNA L.S.

; ALONSO, ARIADNE N.

; MORAES, DANIEL C. DE

; CURVELO, JOSÉ A.R.

; MIRANDA, KILDARE

; PORTELA, MARISTELA B.

; FERREIRA-PEREIRA, ANTÔNIO

; SOUTO-PADRÓN, THAIS

; SOARES, ROSANGELA MARIA DE A.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 N. 3 elocation e20211090

Abstract: En | Text: En | PDF: En

Abstract The search for new therapeutic strategies for leishmaniasis treatment is essential due to the side effects of available drugs and the increasing incidence of resistance to them. Marine sponges use chemical compounds as a defense mechanism, and several of them present interesting pharmacological properties. The aim of this study was to evaluate the in vitro activity of the aqueous extract of the marine sponge Dercitus (Stoeba) latex against Leishmania amazonensis. MIC and toxicity against mammal cells were evaluated through broth microdilution assays. Transmission electron microscopy analysis was performed to assess possible effects on L. amazonensis ultrastructure. Arginase and proteolytic activities were measured by spectrometric methodologies. The extract of Dercitus (Stoeba) latex displayed antileishmanial activity and moderate toxicity against peritonial macrophages. Ultrastructural changes were observed after the growth of L. amazonensis promastigotes in the presence of the extract at 150 µg.ml-1 (IC50), mainly on acidocalcysomes. The extract was able to inhibit the activity of arginase and serine proteases. This study shows that Dercitus (Stoeba) latex aqueous extract may be a novel potential source of protozoa protease inhibitors and drugs that are less toxic to be used in the treatment of L. amazonensis infections.

https://doi.org/10.1590/0001-3765202220211090

Guía clínica de calidad en colonoscopia y polipectomía
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Trujillo-Benavides, Omar

; Solana-Sentíes, Sergio

; Aguilar-Mendoza, Jorge

; Angulo-Molina, Diego

; Barrera-Torres, Hugo

; Barreto-Zúñiga, Rafael

; Betancourt-Linares, Reyes

; Blancas-Valencia, Juan M.

; Cerna-Cardona, Jony

; Espino-Cortés, Héctor

; Hernández-Guerrero, Angélica

; Keil-Ríos, Daniel

; López-Acosta, María Elena

; Martínez-García, Clara L.

; Noble-Lugo, Alejandra

; Peláez-Luna, Mario C.

; Reyes-Dorantes, Ángel A.

; Rojas-Mendoza, Fernando

; Sánchez-Chávez, Xochiquetzal

; Solana-Sentíes, Sergio

; Trujillo-Benavides, Omar E.

; Zamarripa-Dorsey, Felipe

; Zamorano-Orozco, Yolanda

Endoscopia

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jun 2021, Volume 33 N. 2 Pages 54 - 74

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Colonoscopy is the method of choice for diagnosis, and treatment of most colon diseases, being possibly the most effective tool in the prevention and timely detection of colorectal cancer (CRC), influencing its incidence and mortality. Efficacy and diagnostic and therapeutic quality depend on the ability of the endoscopist, percentage of colonic mucosa examined, the examination time, complications, among others. In such a way that even though there are indicators applicable to all colonoscopy, there are other specific ones for each indication. The Mexican Association of Gastroenterology (AMG) and the Mexican Association of Gastrointestinal Endoscopy and Professional College AC (AMEG) held a face-to-face meeting in June 2019, which was attended by gastroenterologists and endoscopists to review and evaluate the current evidence on the quality of CRC screening colonoscopy and develop recommendations that favor the performance of a high-quality colonoscopy.

Resumen La colonoscopia es el método de elección para el diagnóstico y tratamiento de la mayoría de las enfermedades de colon. Es la herramienta más eficaz en la prevención y detección oportuna de cáncer colorrectal (CCR), influyendo en su incidencia y mortalidad. Su eficacia y calidad depende de la habilidad del endoscopista, el porcentaje de área de mucosa examinada, el tiempo de examen y las complicaciones, entre otros. De tal forma que aunque existen indicadores aplicables a toda colonoscopia, existen otros específicos para cada indicación. La Asociación Mexicana de Gastroenterología (AMG) y la Asociación Mexicana de Endoscopia Gastrointestinal y Colegio de Profesionistas A. C. (AMEG) realizaron una reunión presencial en el mes de junio de 2019 a la que asistieron gastroenterólogos y endoscopistas de diversas instituciones del país para revisar y evaluar la evidencia actual sobre calidad en colonoscopia de escrutinio de CCR y desarrollar recomendaciones que favorezcan una colonoscopia de alta calidad.

https://doi.org/10.24875/end.21000012 240 downloads

Qualidade fisiológica, sanitária e enzimática de sementes de arroz irrigado recobertas com silício
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Tunes, Lilian Vanussa Madruga de

; Fonseca, Daniel Ândrei Robe

; Meneghello, Géri Eduardo

; Reis, Bruna Barreto dos

; Brasil, Veronica Duarte

; Rufino, Cassyo de Araújo

; Vilella, Francisco Amaral

Revista Ceres

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oct 2014, Volume 61 N. 5 Pages 675 - 685

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O tratamento de sementes com a utilização de silício em sementes de boa qualidade constitui prática para o aumento da produtividade. O objetivo deste trabalho foi avaliar o efeito do recobrimento de sementes de arroz com duas fontes de silício, em seus atributos fisiológicos, enzimáticos e sanitários. Empregaram-se os cultivares de arroz Irga 424 e Puitá Inta CL e de duas fontes de silício: silicato de alumínio e casca de arroz carbonizada moída, consistindo nas doses de 0; 30; 60; 90 e 120 g 100 kg-1 (de cada produto aplicado) de sementes mais polímero e água, totalizando um volume de calda de 1 L 100 kg-1 de sementes. O delineamento experimental foi o inteiramente casualizado, com quatro repetições. A qualidade fisiológica das sementes foi avaliada no (LAS-FAEM\UFPel) pelos testes de germinação, primeira contagem de germinação, comprimento da parte aérea e raiz, teste de frio e emergência em campo. Para diferenciação isoenzimática, as isoenzimas analisadas foram: esterase, glutamato oxalacetato transaminase e peroxidase, para todos os tratamentos. A avaliação da qualidade sanitária das sementes foi realizada pelo método do papel de filtro ou "Blotter Test". Doses crescentes de casca de arroz carbonizada e de silicato de alumínio, até 120 g 100 kg-1 de sementes, incrementam o vigor de sementes de arroz, avaliados pelo comprimento de raiz e pela emergência a campo. As fontes casca de arroz carbonizada e caulim controlam a incidência de fungos de solo nas sementes de arroz.

Seed treatment using silicon in good quality seeds is a practice for increasing productivity. The objective of this study was to evaluate the effect of coating rice seeds with two sources of silicon ontheir physiological, enzymatic and sanitary traits. This experiment used two rice cultivars, Irga 424 and Puitá Inta CL, and two sources of silicon, aluminum silicate and carbonized rice husk at doses of 0, 30, 60, 90 and 120g 100 kg-1 (of each applied product) of seed plus polymer and water, totaling a spray volume of 1L 100 kg-1 of seeds. The experimental design was completely randomized with four replications. The physiological quality of seeds was evaluated (LAS-FAEM \ UFPel) by germination, first count, length of shoot and root, cold test and field emergence. For isoenzyme differentiation, the following isoenzymes were analyzed: esterase, glutamate oxaloacetate transaminase and peroxidase, for all treatments. The sanitary quality of seeds was evaluated using the filter paper method or "Blotter Test". Increasing doses of carbonized rice husk and aluminum silicate up to g 100 to 120 kg-1 seed incrementedrice seed vigor assessed by the root length and field emergence. The sources of carbonized rice husk and kaolin control the incidence of soil fungi in rice seeds.

https://doi.org/10.1590/0034-737X201461050011 2275 downloads

Antifungal activity of extracts from Piper aduncum leaves prepared by different solvents and extraction techniques against dermatophytes Trichophyton rubrum and Trichophyton interdigitale
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Santos, Maximillan Leite

; Magalhães, Chaiana Froés

; Rosa, Marcelo Barcellos da

; Santos, Daniel de Assis

; Brasileiro, Beatriz Gonçalves

; Carvalho, Leandro Machado de

; Silva, Marcelo Barreto da

; Zani, Carlos Leomar

; Siqueira, Ezequias Pessoa de

; Peres, Rodrigo Loreto

; Andrade, Anderson Assunção

Brazilian Journal of Microbiology

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dec 2013, Volume 44 N. 4 Pages 1275 - 1278

Abstract: En | Text: En | PDF: En

The effects of different solvents and extraction techniques upon the phytochemical profile and anti-Trichophyton activity of extracts from Piper aduncum leaves were evaluated. Extract done by maceration method with ethanol has higher content of sesquiterpenes and antifungal activity. This extract may be useful as an alternative treatment for dermatophytosis.

4036 downloads

Factors influencing growth and intestinal parasitic infections in preschoolers attending philanthropic daycare centers in Salvador, Northeast Region of Brazil
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Lander, Rebecca L.

; Lander, Alastair G.

; Houghton, Lisa

; Williams, Sheila M.

; Costa-Ribeiro, Hugo

; Barreto, Daniel L.

; Mattos, Angela P.

; Gibson, Rosalind S

Cadernos de Saúde Pública

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nov 2012, Volume 28 N. 11 Pages 2177 - 2188

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Déficit de crescimento e parasitoses são comuns entre crianças residentes em periferias. Em estudo transversal com 376 pré-escolares (3-6 anos) de creches em Salvador, Nordeste do Brasil, avaliamos fatores predisponentes para déficit de crescimento e parasitose. Obtiveram-se dados em sete creches sobre peso da criança, altura, nível socioeconômico, estado de saúde e parasitos em amostras de fezes. Prevalência de baixo peso (-1 < DP > -2), desnutrição e baixa estatura foram 12%, 16%, e 6%, respectivamente; nível socioeconômico, ordem de nascimento e peso materno foram preditores da antropometria. Aproximadamente 30% estavam infectados com ≥ 1 parasita. Helmintos (17.8%), notavelmente Trichuris trichiura (12%) e Ascaris lumbricoides (10.5%) e protozoário Giardia duodenalis (13%) foram os mais comuns; < 1% tinha ancilostomíase e Cryptosporidium sp.; 25% apresentaram protozoários cistos não patogênicos. Meninos de famílias muito pobres tiveram menor crescimento e maior risco de helmintose. A desparasitação pode ser considerada uma alternativa para a redução da prevalência de parasitoses intestinais nesse grupo etário.

Poor growth and intestinal parasitic infections are widespread in disadvantaged urban children. This cross-sectional study assessed factors influencing poor growth and intestinal parasites in 376 children aged three to six years in daycare centers in Salvador, in the Northeast Region of Brazil. Data was obtained from seven daycare centers on child weight, height, socio-economic status, health and intestinal parasites in stool samples. Prevalence of moderate underweight (< -1SD > -2SD), wasting and stunting was 12%, 16% and 6% respectively. Socioeconomic status, birth order, and maternal weight were predictors of poor anthropometric status. Almost 30% of children were infected with more than one intestinal parasite. Helminths (17.8%), notably Trichuris trichiura (12%) and Ascaris lumbricoides (10.5%), and protozoan Giardia duodenalis (13%) were the most common types of parasites detected. One percent of children had hookworm and Cryptosporidium sp. and 25% had non-pathogenic protozoan cysts. Boys from families with very low socio-economic status had lower linear growth and presented a greater risk of helminth infection. Deworming is considered an alternative for reducing the prevalence of intestinal parasitic infections in this age group.

8644 downloads

Factors influencing growth and intestinal parasitic infections in preschoolers attending philanthropic daycare centers in Salvador, Northeast Region of Brazil
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Lander, Rebecca L.

; Lander, Alastair G.

; Houghton, Lisa

; Williams, Sheila M.

; Costa-Ribeiro, Hugo

; Barreto, Daniel L.

; Mattos, Angela P.

; Gibson, Rosalind S

Cadernos de Saúde Pública

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nov 2012, Volume 28 N. 11 Pages 2177 - 2188

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

3109 downloads

An ethanol-based process to simultaneously extract and fractionate carotenoids from Mauritia flexuosa L. Pulp
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Ribeiro, Bernardo Dias

; Nascimento, Rafaella Ferreira

; Barreto, Daniel Weingart

; Coelho, Maria Alice Zarur

; Freitas, Suely Pereira

Revista Brasileira de Fruticultura

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sep 2010, Volume 32 N. 3 Pages 657 - 663

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Mauritia vinifera (buriti) é uma palmeira nativa de diferentes regiões do Brasil, particularmente na região Amazônica. O óleo de buriti é rico em carotenóides, especialmente em β-caroteno. A demanda por fontes naturais de β-caroteno tem contribuído para aumentar a industrialização do fruto de buriti pelas usinas de extração de óleos vegetais. O processo mais adotado baseia-se em tecnologias convencionais envolvendo as etapas de despolpamento, secagem e prensagem da polpa para extração do óleo, seguida da separação dos carotenoides em uma fase líquida usando solventes orgânicos. Neste trabalho, foi avaliado o uso do etanol comercial para extração e fracionamento simultâneo de carotenoides a partir da polpa de buriti. A matéria-prima foi misturada com etanol, na proporção 1:4 solvente/substrato, e mantida em banho termostatizado a 60ºC, por 1 hora, sob agitação constante de 30 rpm. A mistura foi filtrada sob vácuo e resfriada a 10ºC resultando na formação de duas fases. A composição de carotenoides foi determinada por CLAE e indicou uma concentração de β-caroteno 12 vezes maior na fase mais densa que na fase leve. O perfil de carotenoides foi similar ao obtido no óleo bruto de buriti, porém a concentração de carotenoides totais foi 40% maior, indicando que o processo tecnológico avaliado é particularmente promissor para aplicação industrial.

Mauritia vinifera (buriti) is a palm tree that grows wild in different areas of Brazil, particularly in the Amazonian region. The buriti oil is rich in carotenoids, especially in β-carotene. The growing interest in other natural sources of β-carotene has stimulated the industrial use of buriti as a raw material for pulp oil extraction. Most processes are based on the conventional technologies, involving drying and pressing the pulp for oil recovery and further separation of carotenoids in a liquid phase using organics solvents. In the present work, the ethanol-based process was evaluated for simultaneous carotenoids recovering and fractionating from buriti pulp. The raw material and ethanol, 1:4 ratio, were placed in an erlenmeyer flask and maintained at 30rpm for 1 hour in a temperature-controlled bath at 65ºC. The mixture was filtered under vacuum and cooling at 10ºC to allow for the separation of the solvent in two phases. Carotenoids composition, determined by HPLC, has indicated a β-carotene concentration about 12 times greater in the lower phase than in the upper phase. The profile of the carotenoids in the denser phase is quite similar to that of raw buriti oil, and the concentration of total carotenoids is 40% higher than that of the original raw oil, making the ethanol-based process particularly attractive for industrial applications.

4114 downloads Cited 1 time in SciELO

Nutritive value of sugarcane silage treated with chemical additives
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Santos, Mateus Castilho

; Nussio, Luiz Gustavo

; Mourão, Gerson Barreto

; Schmidt, Patrick

; Mari, Lucas José

; Ribeiro, José Leonardo

; Queiroz, Oscar Cézar Müller

; Zopollatto, Maity

; Sousa, Daniel de Paula

; Sarturi, Jhones Onorino

; Toledo Filho, Sérgio Gil de

Scientia Agricola

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apr 2009, Volume 66 N. 2 Pages 159 - 163

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A utilização da cana-de-açúcar na forma de silagem constitui-se em um tema que vem se destacando nos últimos anos, e que desperta o interesse de produtores e pesquisadores em função dos benefícios que essa técnica representa. O objetivo do presente trabalho foi avaliar o efeito de aditivos químicos no valor nutritivo da silagem de cana-de-açúcar. O experimento foi desenvolvido em delineamento inteiramente casualizado com quatro repetições por tratamento. Os seguintes aditivos foram utilizados para a confecção das silagens: L. buchneri, cal virgem ou calcário em doses de 1,0 e 1,5% da MV e gesso agrícola a 1,0% da MV, diluídos em 40 litros de água por tonelada de forragem. As variáveis analisadas foram: matéria mineral, proteína bruta, digestibilidade da matéria seca e orgânica, fibra em detergente neutro e fibra em detergente ácido. Para as variáveis de valor nutritivo, as silagens tratadas com cal virgem ou calcário apresentaram, no momento da abertura, maior teor de cinzas, menor concentração de componentes fibrosos e maiores coeficientes digestibilidade da matéria seca e orgânica. O teor de proteína bruta encontrado para esses tratamentos está dentro da amplitude preconizada para a forragem fresca. A ensilagem da cana-de-açúcar com L. buchneri ou gesso apresentaram desempenho semelhante ao tratamento controle para as variáveis acima mencionadas. O tratamento com cal virgem ou calcário melhorou o valor nutritivo da silagem de cana-de-açúcar.

The use of ensiled sugarcane has been increased lately in Brazil due to the benefits that this technique represents. The objective of this study was to evaluate the effects of chemical additives on the nutritive value of sugarcane silages. The trial was carried out in a completely randomized experimental design with four replicates per treatment. The following additives were applied onto the fresh forage before ensiling: L. buchneri, lime or limestone, 1.0 and 1.5% (wet basis) each, and gypsum 1.0% (wet basis), all of them diluted into 40 L of water per ton of fresh weight of forage. The analyzed variables were: ash, crude protein, neutral detergent fiber, acid detergent fiber and organic and dry matter digestibility. The addition of lime or limestone before ensiling produced silages with higher nutritive value compared to all other treatments, due to the increase of the ash content and dry matter and organic digestibility, and also by reducing the fiber content. The crude protein content range was similar to the values observed in the fresh forage. The treatments containing L. buchneri or gypsum were ineffective in improving the nutritive value of sugarcane silages and became similar to the control silages. Treatment containing lime or limestone improved the nutritive value of the sugarcane silage.

4344 downloads Cited 3 times in SciELO

An expeditious and consistent procedure for tetrahydrofuran (THF) drying and deoxygenation by the still apparatus
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Simas, Alessandro B. C.

; Pereira, Vera L. P.

; Barreto Jr., Cleber B.

; Sales, Daniel L. de

; Carvalho, Leandro L. de

Química Nova

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2009, Volume 32 N. 9 Pages 2473 - 2475

Abstract: En | Text: En | PDF: En

Despite the availability of alternative methods for drying tetrahydrofuran (THF), the use of the still apparatus, wherein a THF solution containing Na-bezophenone ketyl is heated to reflux, remains widespread. We herein propose a set of procedures to solve the problems usually faced in applying this drying technique. Moreover, a discussion is made on the chemical knowledge underlying such procedures. Safety and economy issues concerned with the operation of the THF still apparatus are also discussed.

33239 downloads

10.

Acetilação da fibra de bucha (Luffa cylindrica)
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d'Almeida, Ana L. F. S.

; Calado, Verônica

; Barreto, Daniel W.

; d'Almeida, José R. M.

Polímeros

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mar 2005, Volume 15 N. 1 Pages 59 - 62

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Neste trabalho, foi analisado o efeito de um tratamento químico, ou seja, de acetilação de grupos, sobre a estrutura e a morfologia superficial de fibras de bucha (Luffa cylindrica) por meio de FTIR e microscopia eletrônica de varredura. Esse tratamento foi realizado tendo por objetivo aumentar a compatibilidade da bucha com as matrizes poliméricas usualmente empregadas em compósitos. Mostrou-se que o tratamento reduziu a polaridade das moléculas de celulose e removeu a camada superficial das fibras de bucha, expondo a sua estrutura fibrilar interna, com conseqüente aumento da área disponível para a adesão.

In this work the effect of a new chemical surface treatment on the structure and surface morphology of loofah fibers (Luffa cylindrica) was analyzed, using infrared spectroscopy and scanning electron microscopy. The chemical treatment is aimed at increasing loofah compatibility with the polymeric matrices commonly used in composites. It was shown that the treatment used caused a reduction on the polarity of cellulose molecules, and removed the outer surface layer of loofah fibers, exposing their internal fibrillar structure. As a consequence the treatment promoted an increase on the surface area available to adhesion.

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