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Abstract Healthcare workers present an increased risk of contagion for the SARS-CoV-2 virus due to their labor exposure. Here, we describe the clinical, laboratory, and immunological characteristics of healthcare workers, before vaccine application, exposed to SARS-CoV-2-infected patients. We collected sociodemographic, clinical, and laboratory information from 50 professionals who worked during the COVID-19 pandemic at the Clinical Hospital of the Northwest in Brazil. The results showed that most workers are women, over 50 years old, and worked as nursing technicians. Approximately 56% of workers were positive for a previous infection by RT-PCR and/or anti-SARS-CoV-2-immunoglobulin tests. Increased levels of hematocrit, neutrophils, NK lymphocytes, and fibrinogen, were found in positive healthcare workers, suggesting a light inflammatory status. The immunological findings showed an increase in IL-17 production and a Th2/Th17/Th22 profile followed by high serology for anti-SARS-CoV-2 IgM and IgG. Those data reveal the importance of studies with healthcare workers to investigate if the continuous exposition to the virus may result in chronic activation of the immune system and/or pulmonary inflammation in this target group.

https://doi.org/10.1590/0001-3765202320220502

Role of cyclooxygenases 1 and 2 in the maintenance of colonic mucosal integrity in an experimental colitis model
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Costa-Filho, H.B.

; Sales, T.M.A.L.

; Paula, S.M.

; Nicolau, L.A.D.

; Queiroga, M.L.

; Havt, A.

; Soares, P.M.G.

; Barbosa, A.L.R.

; Souza, M.H.L.P.

Brazilian Journal of Medical and Biological Research

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2023, Volume 56 elocation e12946

Abstract: En | Text: En | PDF: En

The role of cyclooxygenase (COXs) isoforms in maintaining colonic mucosal integrity is not fully understood. This study aimed to evaluate the role of COX-1 and -2 on colonic mucosal integrity in an experimental colitis model. Colitis was induced in Wistar rats by intracolonic administration of 2,4,6-trinitrobenzenesulfonic acid (20 mg + 50% ethanol). The control group (sham group) received saline only. After 7, 14, or 28 days, colonic samples were removed, and macroscopic lesion scores, wet weight, myeloperoxidase activity, and transepithelial electrical resistance (TER) were determined. In other rat groups, colonic samples from the sham group and a 7th day post-colitis group were mounted in Üssing chambers with the luminal side exposed to a buffer solution (control), acetylsalicylic acid (ASA), SC-560 (COX-1 inhibitor), or celecoxib (COX-2 inhibitor). TER and epithelial permeability to fluorescein were measured. The 7th day colitis group had higher macroscopic damage scores, wet weight, and myeloperoxidase activity and lower basal TER than the sham, 14th day colitis, and 28th day colitis groups. Inhibition of COX-1 but not COX-2 significantly decreased TER and increased permeability to fluorescein in the 7th day post-colitis group compared to the sham group. Additionally, ASA decreased the colonic mucosal integrity on day seven post-colitis compared to the sham group. A decrease in the colonic mucosa integrity in the experimental colitis model can be aggravated only by the inhibition of COX-1, which demonstrated the importance of this enzyme in the maintenance of colonic mucosal integrity.

https://doi.org/10.1590/1414-431x2023e12946

Proposal of an iELISA for Mycoplasma bovis diagnosis in dairy cattle and associated risk factors
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Pires, D.R.

; Morais, A.C.N.

; Cunha, N.C.

; Machado, L.S.

; Barbosa, L.F.C.

; Mendonça, J.F.M.

; Balaro, M.F.A.

; Santos, J.P.C.

; Souza, G.N.

; Barreto, M.L.

; Nascimento, E.R.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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mar 2021, Volume 73 N. 2 Pages 293 - 301

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Mycoplasma bovis é um agente altamente contagioso relacionado a várias patologias em bovinos. A detecção de anticorpos reativos a M. bovis por Ensaio de Imunoadsorção Enzimática Indireto (iELISA) identifica se houve exposição ao microrganismo. O presente estudo teve como objetivo otimizar um iELISA de antígeno celular total de M. bovis, aplicando-o a amostras de soro bovino, bem como avaliar fatores de risco. Amostras de soro foram obtidas de 400 vacas de 17 rebanhos da Região Sudeste do Brasil. Na otimização do iELISA foram obtidos: 2μg/mL de antígeno, diluição dos soros 1:300 e do conjugado 1:15000. A frequência de animais reativos foi de 62,3% (249/400) e de 100% (17/17) para os rebanhos. Os fatores de risco foram: rebanhos com mais de 100 animais (OR= 3,1; IC= 95%); raça Holandesa (OR= 72,5;IC= 95%); vacas (OR= 29,7;IC= 95%); sistema intensivo (OR= 3,3; C= 95%); produção de pequenos ruminantes (OR= 4,4;IC=95%); produção de leite acima de 500L (OR= 2,9;IC= 95%); sem quarentena (OR= 1,5;IC= 95%); ordenha mecânica (OR= 5,5;IC= 95%) e casos de mastite (OR= 5,5;IC= 95%). O iELISA proposto foi capaz de detectar anticorpos reativos a M. bovis no soro bovino. O conhecimento desses fatores de risco pode auxiliar na implementação de medidas profiláticas.

ABSTRACT Mycoplasma bovis is a highly contagious agent associated with several pathologies in cattle. The detection of reactive antibodies to M. bovis by Indirect Enzyme-Linked Immunosorbent Assay (iELISA) identifies if there was an exposure to the microorganism. The current study aimed to optimize an iELISA from M. bovis total cell antigen, applying it to bovine serum samples, and to evaluate risk factors. Serum samples were obtained from 400 cows from 17 herds from Southeast Brazil. In the optimization of iELISA, the following was established: 2 μg/mL of antigen, sera dilution 1:300, and conjugate dilution 1:15000. The frequency was 62.3% (249/400) of reactive animals and 100% (17/17) of reactive herds. Risk factors were: herds with more than 100 animals (OR= 3.1; CI= 95%); Holstein breed (OR= 72.5; CI= 95%); cows (OR= 29.7; CI= 95%); intensive breeding system (OR= 3.3; CI= 95%); associated small ruminant production (OR= 4.4; CI= 95%); milk production above 500L (OR= 2.9; CI= 95%); no quarantine (OR= 1.5; CI= 95%); mechanical milking (OR= 5.5; CI= 95%) and cases of mastitis (OR= 5.5; CI= 95%). The proposed iELISA was able to detect antibodies reactive to M. bovis in bovine serum. Knowledge of these risk factors can assist in the implementation of prophylactic measures.

https://doi.org/10.1590/1678-4162-12140 297 downloads

Diferentes momentos de aplicação de gonadotrofina coriônica equina em protocolo de inseminação artificial em tempo fixo para vacas de leite
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Araújo, M.L.

; Barbosa, L.P.

; Biscarde, C.E.A.

; Mendes, C.S.

; Lents, M.P.

; Pinheiro, E.E.G.

; França, C.S.

; Jesus, R.D.L.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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dec 2019, Volume 71 N. 6 Pages 1934 - 1939

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Avaliaram-se diferentes momentos de aplicação da gonadotrofina coriônica equina (eCG) em protocolos de inseminação artificial em tempo fixo (IATF) para vacas de leite. Foram utilizadas 76 fêmeas, as quais receberam, no dia zero (D0) do protocolo, dispositivos intravaginais de progesterona, sendo esses retirados no D9, e os animais foram, então, distribuídos aleatoriamente em três tratamentos: T1 - aplicação de eCG no momento da retirada dos dispositivos; T2 e T3 - aplicação de eCG 48h e 24h antes da retirada dos dispositivos, respectivamente. No D10 os animais receberam 1mg de GnRH, e a IATF foi realizada 52 horas após a retirada do implante. Não houve diferença (P>0,05) para intervalo entre a retirada do implante à ovulação (72,56±3,92h), o diâmetro do maior folículo no D9 (10,88±1,49mm), o diâmetro do folículo ovulatório (15,15±1,16mm) e do segundo maior folículo (7,49±0,52mm), a taxa de crescimento folicular (1,38±0,04mm/dia), a taxa de ovulação (96,67%), o intervalo entre diâmetro final e inicial do folículo dominante (73,49±3,84h), a área de corpo lúteo (2,27±0,43cm²), a porcentagem de CL no ovário direito (53,00%) e no esquerdo (26,33%) e a taxa de gestação (33,33%). O momento da aplicação da eCG não influenciou na eficiência do protocolo. Recomenda-se a utilização da eCG no momento da retirada do implante por otimização do manejo.

ABSTRACT Different times of application of equine chorionic gonadotropin (eCG) in Fixed-Time Artificial Insemination (FTAI) protocols for dairy cows were evaluated. A total of 76 females were used, which received intravaginal progesterone devices on day zero (D0) of the protocol, which were withdrawn on D9, and the animals were then randomly distributed in three treatments: T1- application of eCG at the time of device withdrawal; T2 and T3 - application of eCG 48h and 24h before withdrawal of the devices, respectively. On D10 the animals received 1mg of GnRH and the FTAI was carried out 52 hours after the removal of the implant. There was no difference (P>0.05) for interval from implant removal to ovulation (72.56±3.92h), diameter of the largest follicle of D9 (10.88±1.49mm), diameters of ovulatory follicle (15.15±1.16mm) and of the second largest follicle (7.49±0.52mm), follicle growth rate (1.38±0.04mm/day), ovulation rate (96.67%), interval between final diameter and initial diameter of dominant follicle (73.49±3.84h), corpus luteum area (2.27±0.43cm²), percentage of CL in right ovary (53.00%) and in left ovary (26.33%) and pregnancy rate (33.33%). The time of the application of eCG did not influence the efficiency of the protocol. We recommend the use of eCG at the time of the removal of the implant for management optimization.

https://doi.org/10.1590/1678-4162-10282 1115 downloads

Qualidade seminal e perfil metabólico de caprinos alimentados com semente de linhaça na dieta
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Souza, R.S.

; Barbosa, L.P.

; Pinheiro, A.M.

; Machado, W.M.

; Mendes, C.S.

; Araujo, M.L.

; Souza, D.O.

; Santana, A.L.A.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jun 2019, Volume 71 N. 3 Pages 899 - 908

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo da presente pesquisa foi avaliar o efeito da inclusão de semente de linhaça na dieta sobre a qualidade seminal e o perfil metabólico de machos caprinos. Foram utilizados 16 machos da raça Saanen, distribuídos aleatoriamente em quatro grupos, recebendo níveis de inclusão de semente de linhaça (0, 4, 8 e 12%) na dieta, durante um período de 60 dias. As coletas e as avaliações seminais foram realizadas duas vezes por semana. Os animais foram submetidos a coletas quinzenais de sangue, durante todo o período experimental. Os dados foram avaliados por ANOVA e pela análise de regressão a 5% de significância. Houve comportamento cúbico para motilidade espermática progressiva, que apresentou o maior valor (82,30%) com a adição de 9,92% de semente de linhaça na dieta (P<0,05). Houve comportamento cúbico para concentração plasmática de triglicerídeos, e o nível de 8% de semente de linhaça na dieta apresentou média de 43,32mg dL-1, acima dos valores normais para a espécie caprina (P<0,05). A inclusão de semente de linhaça na dieta de machos caprinos proporcionou melhora na produção espermática e na qualidade seminal. No entanto, devem-se considerar os possíveis efeitos dos níveis superiores a 4% de semente de linhaça sobre o perfil metabólico dos animais.

ABSTRACT This study aimed to evaluate the effect of flaxseed inclusion in the diet of male goats through the semen quality and metabolic profile. Randomly allocated 16 Saanen males were placed into four groups receiving flaxseed inclusion levels (0, 4, 8 and 12%) in the diet over a period of 60 days. The collections and seminal evaluations were performed twice a week. The animals were subjected to biweekly blood collections from the beginning to the end of the trial period. The data were evaluated by ANOVA and regression analysis at 5% significance. A cubic behavior for motility, with the highest value (82.30%) with the addition of 9.92% of flaxseed in the diet (P< 0.05) was detected. A cubic behavior for plasma triglyceride concentration and the level of 8% of flaxseed in the diet averaged 43.32mg dL -1 above normal values for goats (P< 0.05) was detected. The inclusion of flaxseed in the diet of male goats improved sperm production and quality. However, one should consider the possible effects of the levels above 4% of flaxseed on the metabolic profile of the animals.

https://doi.org/10.1590/1678-4162-10266 983 downloads

Ramos colaterais do Arco aórtico do preá (Galea spixii Wagler, 1831)
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Oliveira, Radan E.M.

; Oliveira, Gleidson B.

; Barbosa, Procássia M.L.

; Bezerra, Ferdinando V.F.

; Albuquerque, José Fernando Gomes de

; Ambrósio, Carlos E.

; Miglino, Maria A.

; Oliveira, Moacir F.

Pesquisa Veterinária Brasileira

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aug 2015, Volume 35 N. 8 Pages 762 - 766

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo: O preá é um roedor típico da caatinga pertencente à família Caviidae. Considerando a inexistência de dados sobre o arco aórtico do preá, foi realizado este estudo tendo como objetivo descrever os ramos colaterais do arco aórtico neste cavídeo, e dessa forma, contribuir com dados para biologia da espécie. Foram utilizados vinte preás machos provenientes de estudos anteriores e encontravam-se armazenados em freezer no Centro de Multiplicação de Animais Silvestres (CEMAS/UFERSA). Os animais foram descongelados, a cavidade torácica foi aberta, a aorta canulada e o sistema vascular lavado com solução salina e em seguida, injetado látex Neoprene corado com pigmento vermelho, amarelo ou branco. Posteriormente, os animais foram fixados em formol e depois de 72 horas, dissecados e analisados, sendo obtidos desenhos esquemáticos e os exemplares mais representativos fotografados. O arco aórtico do preá emitiu como ramos colaterais, o tronco braquiocefálico e a artéria subclávia esquerda. O tronco braquiocefálico originou na maioria das peças estudadas, a artéria carótida comum esquerda e o tronco braquiocarotídeo, do qual surgem as artérias subclávia direita e carótida comum direita. As artérias subclávias direita e esquerda em todos os animais estudados emitiram a artéria vertebral, a artéria torácica interna, a artéria cervical superficial, o tronco costocervical e a artéria axilar. O padrão da formação do arco aórtico do preá assemelhou-se ao observado em outros roedores, tais como no mocó, no porquinho-da-índia e na chinchila.

Abstract: Galea spixii is a typical rodent of the caatinga belonging to the Caviidae family. Considering the lack of data on the aortic arch of the galea, this study aimed to describe the collateral branches of the aortic arch in this specie and to contributes to knowledge of its biology. Twenty males from previous studies were used which had been stored in a freezer at Multiplication Center of Wild Animals (CEMAS/UFERSA). The animals were thawed, the thoracic cavity was opened and the aorta cannulated vasculature was washed with saline and then injected with Neoprene latex colored with red pigment, yellow or white. Subsequently, the animals were fixed in formalin and after 72 hours dissected and analyzed, to obtain schematic drawings and the most representative samples were photographed. The cavy aortic arch issued as collateral branches, the brachiocephalic trunk and the left subclavian artery. The brachiocephalic trunk afforded in most of the specimens the left common carotid artery and the brachiocarotid trunk, where the right subclavian and right common carotid arteries arise. The right and left subclavian arteries in all animals studied issued the vertebral artery, internal thoracic artery, superficial cervical artery costocervical trunk and axillary artery. The pattern of formation of the aortic arch of galea was similar to that observed in other rodents, such as the kerodon, the guinea pig and chinchilla.

https://doi.org/10.1590/S0100-736X2015000800008 1699 downloads

Morfometria do aparelho genital e resposta superovulatória de coelhas suplementadas com geleia real
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Dutra, P.A.

; Barbosa, L.P.

; Santana, A.L.A.

; Aguiar, C.S.

; Souza, R.S.

; Cardoso Neto, B.M.

; Araújo, M.L.

; Araújo, R.C.S.A.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jun 2013, Volume 65 N. 3 Pages 699 - 704

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Avaliou-se o efeito da suplementação com geleia real sobre a morfometria do aparelho genital, resposta superovulatória e qualidade embrionária de coelhas. Trinta e seis fêmeas foram distribuídas em quatro grupos (G), sendo: G1 (n=9) formado por animais não suplementados com geleia real, e G2, G3 e G4 (n=9 em cada grupo) por animais suplementados com 10, 20 e 40mg/dia de geleia real. A superovulação consistiu na aplicação de 40UI de gonadotrofina coriônica equina, seguida por 40UI de gonadotrofina coriônica humana, via intramuscular, 48 horas após, e submetidas à cobrição natural. Os animais foram sacrificados, e os embriões coletados 72 horas após a cópula. Não houve diferença estatística entre tratamentos para as variáveis analisadas. O peso médio do aparelho genital foi de 10,88±0,38g; dos ovários - direito e esquerdo -, 0,28±0,02g; e o índice gonadossomático, 0,02±0,0g. O número médio de estruturas totais recuperadas foi de 9,2±1,4; de embriões viáveis, 8,7±1,4; e de degenerados, 0,5±0,2. Dos embriões viáveis, 5,6±0,8 foram classificados como grau I; 2,3±0,5, como grau II; e 0,8±0,2, como grau III. A suplementação com geleia real na dose de até 40mg/dia não apresentou efeito estimulador sobre o aparelho genital e a qualidade embrionária de coelhas.

The purpose of this paper was to assess the effect of supplementation of royal jelly on the morphometry of the genital tract, superovulatory response and embryonic quality in adult rabbits. 36 rabbits were distributed in four groups (G), as follows: G1 (n=9): no supplementation with royal jelly, G2, 3 and 4 (n=9): supplementation with 10, 20 and 40mg/day of royal jelly. The superovulatory protocol consisted in the application of a dose of 40UI of Equine Chorionic Gonadotrophin, followed by a dose of UI of Human Chorionic Gonadotrophin, intramuscularly, 48 hours later, and submitted to natural cover. The animals were euthanasiated and the embryos collected 72 hours after copulation. There was no statistical difference for the analyzed variables (P>0.05). The average weight for the female genital tract was 10.89±0.38g; the average weight of right and left ovaries was 0.28±0.02g and the average gonadosomatic index rate was 0.02±0.0g. The number of total recovered structures was 9.2±1.4. The average number of viable embryos was 8.7±1.4 and of degenerated embryos was 0.5±0.2. Viable embryos were morphologically classified as degree I: 5.6±0.8; degree II: 2.3±0.5; degree III: 0.8±0.2. Royal jelly did not present stimulatory effect on organs related to the reproduction of rabbits, nor was it effective to improve their embryonic quality at dosages of up to 40mg of in natura royal jelly.

2541 downloads

Farelo de manga na dieta de cordeiros em confinamento
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Aragão, A.S.L.

; Pereira, L.G.R.

; Chizzotti, M.L.

; Voltolini, T.V.

; Azevêdo, J.A.G.

; Barbosa, L.D.

; Santos, R.D.

; Araújo, G.G.L.

; Brandão, L.G.N.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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aug 2012, Volume 64 N. 4 Pages 967 - 973

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Avaliou-se o efeito da substituição do milho por farelo de manga em dietas para ovinos. As características avaliadas foram: consumo e digestibilidade aparente da matéria seca (MS), da proteína bruta (PB), da fibra em detergente neutro (FDN), da matéria orgânica (MO), do extrato etéreo (EE), da energia bruta (EB), da energia digestível (ED), dos carboidratos não fibrosos (CNF) e dos carboidratos totais (CT). Vinte e quatro cordeiros Santa Inês foram distribuídos em delineamento inteiramente ao acaso, com quatro tratamentos, constituídos pelas porcentagens de substituição do milho pelo farelo de manga - 0, 33, 66 e 100% -, com seis repetições. A matéria seca fecal foi estimada utilizando-se o indicador LIPE®. Não houve efeito da inclusão do farelo de manga sobre o consumo dos nutrientes e nos coeficientes de digestibilidade. Os consumos de EB e ED, em kcal/gMS, aumentaram linearmente com a inclusão do farelo de manga. O farelo de manga pode substituir o milho em dietas para ovinos, sem comprometer o consumo e a digestibilidade dos nutrientes, indicando a possibilidade de uso na dieta desses animais como alimento alternativo.

The effect of replacing corn meal with mango meal in diets of feedlot lambs was evaluated. Apparent digestibility and intakes of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), organic matter (OM) (EE), gross energy (GE), digestible energy (DE), non-fiber carbohydrates (NFC) and total carbohydrates (TC) were evaluated. Twenty-four Santa Inês lambs were distributed in a randomized design with four treatments, consisting of the replacement levels of corn meal by mango (0, 33, 66 and 100%) with six replicates. The fecal dry matter was estimated using LIPE ® external marker. There is no effect of mango meal inclusion levels on the intake and apparent digestibility. The intakes of GE and DE in kcal/gDM increase linearly with mango meal inclusion. The mango meal can replace corn in diets for lambs without compromising the intake and digestibility of nutrients, indicating potential as an alternative feed.

2854 downloads Cited 1 time in SciELO

Participation of the NO/cGMP/K+ATP pathway in the antinociception induced by Walker tumor bearing in rats
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Barbosa, A.L.R.

; Pinheiro, C.A.

; Oliveira, G.J.

; Torres, J.N.L.

; Moraes, M.O.

; Ribeiro, R.A.

; Vale, M.L.

; Souza, M.H.L.P.

Brazilian Journal of Medical and Biological Research

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jun 2012, Volume 45 N. 6 Pages 531 - 536

Abstract: En | Text: En | PDF: En

Implantation of Walker 256 tumor decreases acute systemic inflammation in rats. Inflammatory hyperalgesia is one of the most important events of acute inflammation. The L-arginine/NO/cGMP/K+ATP pathway has been proposed as the mechanism of peripheral antinociception mediated by several drugs and physical exercise. The objective of this study was to investigate a possible involvement of the NO/cGMP/K+ATP pathway in antinociception induced in Walker 256 tumor-bearing male Wistar rats (180-220 g). The groups consisted of 5-6 animals. Mechanical inflammatory hypernociception was evaluated using an electronic version of the von Frey test. Walker tumor (4th and 7th day post-implantation) reduced prostaglandin E2- (PGE2, 400 ng/paw; 50 µL; intraplantar injection) and carrageenan-induced hypernociception (500 µg/paw; 100 µL; intraplantar injection). Walker tumor-induced analgesia was reversed (99.3% for carrageenan and 77.2% for PGE2) by a selective inhibitor of nitric oxide synthase (L-NAME; 90 mg/kg, ip) and L-arginine (200 mg/kg, ip), which prevented (80% for carrageenan and 65% for PGE2) the effect of L-NAME. Treatment with the soluble guanylyl cyclase inhibitor ODQ (100% for carrageenan and 95% for PGE2; 8 µg/paw) and the ATP-sensitive K+ channel (KATP) blocker glibenclamide (87.5% for carrageenan and 100% for PGE2; 160 µg/paw) reversed the antinociceptive effect of tumor bearing in a statistically significant manner (P < 0.05). The present study confirmed an intrinsic peripheral antinociceptive effect of Walker tumor bearing in rats. This antinociceptive effect seemed to be mediated by activation of the NO/cGMP pathway followed by the opening of KATP channels.

1911 downloads

10.

Virulence exaltation of Clostridium perfringens strains from bovines
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Baldassi, L.

; Barbosa, M.L.

; Bach, E.E.

; Iaria, S.T.

Journal of Venomous Animals and Toxins including Tropical Diseases

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2004, Volume 10 N. 3 Pages 280 - 292

Abstract: En | Text: En | PDF: En

Ten out of eighty-nine strains biochemically identified as Clostridium perfringens, isolated from bovine organs, were selected by their different results showed in toxigenicity test on mice. Those and the standard strains, ATCC types A, B, C, and D, had their virulence exalted through serial intramuscular inoculation into guinea pigs. Results showed that, for toxigenic strains (6), one or two passages were enough to cause exaltation, while for the atoxigenic (4), five or six inoculations were needed. Esterase electrophoresis of standard and isolated strains, with and without exaltation, was performed. Electrophoresis analysis permits the following conclusions: strains that do not show any clinical symptoms in mice, when exalted demonstrate decreased esterase activity; on the contrary, it is increased when correlated with animal symptoms.

2493 downloads

11.

Esterase electrophoresis of Clostridium perfringens bovine strains
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Baldassi, L.

; Barbosa, M.L.

; Bach, E.E.

; Iaria, S.T.

Journal of Venomous Animals and Toxins including Tropical Diseases

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2003, Volume 9 N. 2 Pages 277 - 290

Abstract: En | Text: En | PDF: En

Eighty-nine of 144 isolates of Clostridium perfringens obtained from 187 samples of 71 bovine in several Brazilian states were submitted to esterase electrophoresis for typing. Mobilites electrophoresis, as parameter, were settled down by isolates from ATCC pattern of types A, B, C, and D. Of the 89 isolates, 43 (48.3%) were characterized as electrophoretic type A, 20 (22.5%) as D, 18 (20.2%) as C, and 3 (3.4%) as B. Five (5.6%) isolates did not identity with any type. Similarly, esterase electrophoresis enabled the typing of 94.4 % of the isolates, demonstrating to be an appropriate method for animal sample analyses.

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