Abstract The objective of the present work was to quantify the effect of phenolic derivatives on in the oral mucosa of university laboratory technicians. Twenty (20) university laboratory technicians exposed to agents and 11 non-smokers without occupational exposure to phenolic derivatives for more than 10 years underwent anamnesis and extra and intra-oral clinical examination. Exfoliative cytology of the right buccal mucosa was performed. The frequency of micronuclei formation (MN) was analyzed in 1200 cells by standard microscopy. Mann-Whitney and Kruskal-Wallis test were used. The difference between the groups evaluated was observed in the frequency of more than 01 MN/cell (p=0.0397), being more present in the exposed group. The use of personal protective equipment contributed to a decrease in the frequency of 01 MN/cell (p=0.0272) for the exposed group. Genotoxic effects may be reduced by the use of PPE and/or exhaust laminar flow hood. Five years of exposure could adapt the nucleus cells to the genotoxic aggressor demonstrating that the oral mucosa could be a marker to long-term genotoxic exposure.

Resumo O objetivo deste estudo foi examinar as taxas de edentulismo no Brasil e fazer projeções para os próximos anos. Os dados foram coletados de três censos nacionais de saúde bucal. O percentual de arcadas edêntulas foi calculado. Foram feitas projeções para 2020, 2030 e 2040, assumindo que o edentulismo segue uma função logística. Também foram realizadas projeções populacionais. A variação anual da proporção de arcadas edêntulas foi de -0,04% para jovens, -0,96% para adultos e 0,76% para idosos. Até 2040, o percentual de arcadas edêntulas será virtualmente zero entre jovens, 1,77% entre adultos e 85,96% entre idosos. O número de jovens vai diminuir levemente; o de adultos vai aumentar e depois diminuir; e o de idosos vai continuar aumentando. Para jovens e adultos, o número de arcadas edêntulas vai diminuir, aproximando-se de 616.000 em 2040. Para os idosos, este número vai aumentar alarmantemente, alcançando mais de 64 milhões em 2040. O edentulismo está diminuindo no Brasil entre jovens e adultos, mas está aumentando e continuará a aumentar nas próximas décadas entre os idosos.

Abstract The aim of this study was to examine the edentulism rates in Brazil and make projections for the next years. Data were collected from three national oral health surveys. The percentage of edentulous jaws was calculated. Projections were made for the years 2020, 2030 and 2040, assuming that edentulism follows a logistic function. Population projections were also performed. Annual change in proportion of edentulous jaws was -0.04% for teenagers, -0.96% for adults and 0.76% for the elderly. By 2040, edentulous jaws will be virtually zero among teenagers, 1.77% among adults and 85.96% among the elderly. Teenagers will slightly decrease in number; adults will increase and subsequently decrease; the elderly will continue to increase. In teenagers and adults, the number of edentulous jaws will decrease, being approximately 616,000 in 2040. In the elderly, it will increase alarmingly, reaching over 64 million in 2040. Edentulism is declining in Brazil among teenagers and middle-aged adults, but is still increasing and will continue to increase for the next decades among the elderly.

Resumo O objetivo deste estudo foi examinar as taxas de edentulismo no Brasil e fazer projeções para os próximos anos. Os dados foram coletados de três censos nacionais de saúde bucal. O percentual de arcadas edêntulas foi calculado. Foram feitas projeções para 2020, 2030 e 2040, assumindo que o edentulismo segue uma função logística. Também foram realizadas projeções populacionais. A variação anual da proporção de arcadas edêntulas foi de -0,04% para jovens, -0,96% para adultos e 0,76% para idosos. Até 2040, o percentual de arcadas edêntulas será virtualmente zero entre jovens, 1,77% entre adultos e 85,96% entre idosos. O número de jovens vai diminuir levemente; o de adultos vai aumentar e depois diminuir; e o de idosos vai continuar aumentando. Para jovens e adultos, o número de arcadas edêntulas vai diminuir, aproximando-se de 616.000 em 2040. Para os idosos, este número vai aumentar alarmantemente, alcançando mais de 64 milhões em 2040. O edentulismo está diminuindo no Brasil entre jovens e adultos, mas está aumentando e continuará a aumentar nas próximas décadas entre os idosos.

Abstract The aim of this study was to examine the edentulism rates in Brazil and make projections for the next years. Data were collected from three national oral health surveys. The percentage of edentulous jaws was calculated. Projections were made for the years 2020, 2030 and 2040, assuming that edentulism follows a logistic function. Population projections were also performed. Annual change in proportion of edentulous jaws was -0.04% for teenagers, -0.96% for adults and 0.76% for the elderly. By 2040, edentulous jaws will be virtually zero among teenagers, 1.77% among adults and 85.96% among the elderly. Teenagers will slightly decrease in number; adults will increase and subsequently decrease; the elderly will continue to increase. In teenagers and adults, the number of edentulous jaws will decrease, being approximately 616,000 in 2040. In the elderly, it will increase alarmingly, reaching over 64 million in 2040. Edentulism is declining in Brazil among teenagers and middle-aged adults, but is still increasing and will continue to increase for the next decades among the elderly.

INTRODUÇÃO: O controle das microdeformações ao redor dos implantes é um fator que pode levar a uma melhor osseointegração e manutenção das estruturas ósseas.OBJETIVO: Avaliar a distribuição de tensões ao redor dos implantes,utilizando-se a extensometria, sob a influência da aplicação de cargas estáticas axiais e não axiais em próteses parciais fixas de três elementos, em diferentes pontos de aplicação de carga.MATERIAL E MÉTODO: Em um bloco de poliuretano, foram instalados três implantes de hexágono interno e, sobre esses implantes, pilares protéticos microunit foram conectados com torque de 20 Ncm. Cilindros plásticos foram utilizados para realização dos enceramentos padronizados que, posteriormente, foram fundidos em liga de cobalto cromo. Extensômetros foram colados na superfície do bloco ao redor dos três implantes. As estruturas metálicas foram parafusadas com torque de 10 Ncm cada. Em seguida, foram realizados carregamentos verticais estáticos de 30 kgf, durante dez segundos, em cinco pontos pré-determinados (A, B, C, D e E), utilizando um dispositivo de aplicação de cargas (DAC).RESULTADO: Os dados foram analisados pelo teste RM ANOVA, que indicou que o efeito entre o ponto de aplicação de carga e os diferentes corpos de prova não foi estatisticamente significante, enquanto que, para o efeito entre os pontos de aplicação de carga, houve diferença (p=0,0001). Em seguida, aplicou-se o teste de comparação múltipla de Tukey.CONCLUSÃO: A aplicação de carga sobre os pontos não axiais D e E produziu um aumento da magnitude de microdeformação ao redor dos implantes.

INTRODUCTION: The control of the micro strain surrounding implants is a factor that can lead to a better osseointegration and maintenance of bone structures.OBJECTIVE: evaluate in vitro, using strain gauge analysis , the influence of the axial and non-axial static load in implant-supported fixed partial dentures, varying the point of load application.MATERIAL AND METHOD: In a block of polyurethane three internal hexagonal implants were installed, on implants abutments microunit were connected with torque of 20 Ncm, and plastic prosthetic cylinders were screwed on to the abutments, which received standard patterns cast in Co-Cr alloy (n=10). Four strain gauges were bonded on the surface of the block tangentially to the three implants. Each metallic structure was screwed onto the microunit with a 10 Ncm torque. With a load application device (DAC) static vertical loads of 30kgf were applied for ten seconds at five predetermined points (A, B, C, D and E).RESULT: Result analyzed by RM ANOVA, in which the effect of the point of load application and differents specimens were not statistically significant, whereas regarding the points of load application presented statistical differenced (p=0.0001). Applying Tukey's multiple comparison tests.CONCLUSION: The application of load on the non-axial points D and E produced an increase in the magnitude of micro strain around implants.

AIM: To evaluate the bonding durability of materials used as sealants on different dentitions. METHODS: Deciduous (D) and permanent (P) molars were divided into four groups (n=5) and sealed with Fluroshield (F) and OptiBond FL (FL). Blocks of composite resin were built, stored in distilled water (24 h/37 °C), sectioned into 0.8 mm2 and tested at tensile (0.5 mm/min) after 24 h and 6 months. The fractures were observed and classified into adhesive, cohesive or mixed types. The data were analyzed using repeated measures ANOVA and Tukey test (α=5%), with tooth as the experimental unit. RESULTS: There were differences for dentition (p=0.0097), dental sealant (p=0.0019) and time (p=0.0001). At 24h the highest bond strength was observed for OptiBond FL on deciduous and permanent teeth, similar to Fluroshield at both dentitions. After 6 months the bond strength of OptiBond FL did not decrease in the permanent teeth, but decreased similar to Fluroshield in deciduous teeth, the bond strength of Fluroshield decreased similarly in both dentitions. CONCLUSIONS: The bond strength of dental sealants varied with the experimental conditions.

AIM: To evaluate the influence of ovariectomy combined with lack of masticatory force in the evolution of periodontal disease induced in rats. METHODS: Forty rats were bilaterally ovariectomized and 40 were submitted to sham ovariectomy. Periodontal disease was induced in the mandibular left first molar and the maxillary left first molar was extracted from half of the rats. The rats were randomly euthanized at 3, 7, 14 and 30 days post periodontal disease induction. Serial sections were obtained from the furcation area and stained for histological and histomorphometric analysis. The results of the histomorphometric analysis were statistically analyzed by ANOVA and Tukey tests. RESULTS: The results demonstrated statistically significant differences in the percentage of bone tissue when the variables presence or absence of estrogen (p=0.020) and time of euthanasia (p=0.002) were evaluated. However, the extraction procedure did not significantly affect the percentage of bone tissue (p=0.598). CONCLUSIONS : The bone loss resulting from periodontal disease is increased by estrogen deficiency and varies according to the time course of periodontitis. In contrast, masticatory force does not seem to interfere in bone loss derived from periodontal disease.

Volatile sulphur compounds (VSC) are the gases mainly responsible for halitosis (bad breath). The aim of this research was to evaluate the effects of medicinal plants on halitosis control. Two commonly used plants were tested: Curcuma zedoaria and Camellia sinensis (green tea). These plants were prepared as an aqueous solution and used as mouthwashes, compared with a standard mouthwash of 0.12% chlorhexidine gluconate and a placebo (water). The experiment was conducted with 30 volunteers from the School of Dentistry of São Jose dos Campos, Univ. Estadual Paulista - UNESP, SP, Brazil. Each volunteer tested the four mouthwashes. The Cysteine Challenge Method, modified for this study, was used for initial breath standardization. Four breath assessments were conducted after volunteers rinsed orally with acetylcysteine: one before the test mouthwash was used; the second, one minute after its use; a third 90 minutes later; and the last 180 minutes later. The results showed that chlorhexidine gluconate lowered VSC production immediately, and that this effect lasted up to 3 hours, while the tested plants had immediate inhibitory effects but no residual inhibitory effects on VSC. We concluded that Curcuma zedoaria and Camellia sinensis, prepared as infusions and used as mouthwashes, did not have a residual neutralizing effect on VSC.

The aim of this study was to evaluate the effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10% sodium thiosulphate solution (Na2S2O3) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100% of the microbial inocula were eliminated. It was concluded that the 2% peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure.

OBJECTIVES: The aim of this study was to evaluate the influence of two adhesive systems and the post space region on the degree of conversion of dual resin cement and its bond strength to root dentin. MATERIAL AND METHODS: One three-step etch-and-rinse (All-bond 2, Bisco) and another one-step self-etch (Xeno III, Dentsply) adhesive systems were applied on 20 (n=10) crownless bovine incisors, at 12-mm-deep post space preparation, and a fiber post (FRC Postec, Ivoclar) was cemented using a dual cure resin cement (Duo-Link, Bisco). Three transverse sections (3 mm) were obtained, being one from each study region (cervical, middle and apical). The degree of conversion of the dual cure resin cement was determined by a micro-Raman spectrometer. The data (%) were submitted to repeated-measures analysis of variance and Tukey's test (p<0.05). RESULTS: For both groups, the degree of conversion means (%) (All bond 2cervical = 69.3; All bond 2middle = 55.1; All bond 2apical= 56; Xeno III cervical = 68.7; Xeno IIImiddle = 68.8; Xeno III apical = 54.3) were not significantly different along the post space regions (p<0.05). CONCLUSION: Neither the adhesive nor the post space region influenced the degree of conversion of the cement layer.

OBJECTIVE: Using strain gauge (SG) analysis, the aim of this in vitro study was quantify the strain development during the fixation of three-unit screw implant-supported fixed partial dentures, varying the types of implant-abutment joints and the type of prosthetic coping. The hypotheses were that the type of hexagonal connection would generate different microstrains and the type of copings would produce similar microstrains after prosthetic screws had been tightened onto microunit abutments. MATERIALS AND METHODS: Three dental implants with external (EH) and internal (IH) hexagonal configurations were inserted into two polyurethane blocks. Microunit abutments were screwed onto their respective implant groups, applying a torque of 20 Ncm. Machined Co-Cr copings (M) and plastic prosthetic copings (P) were screwed onto the abutments, which received standard wax patterns. The wax patterns were cast in Co-Cr alloy (n=5), forming four groups: G1) EH/M; G2) EH/P; G3) IH/M and G4) IH/P. Four SGs were bonded onto the surface of the block tangentially to the implants, SG 1 mesially to implant 1, SG 2 and SG 3 mesially and distally to implant 2, respectively, and SG 4 distally to implant 3. The superstructure's occlusal screws were tightened onto microunit abutments with 10 Ncm torque using a manual torque driver. The magnitude of microstrain on each SG was recorded in units of microstrain (µε). The data were analyzed statistically by ANOVA and Tukey's test (p<0.05). RESULTS: Microstrain values of each group were: G1= 338.1±223.0 µε; G2= 363.9±190.9 µε; G3= 415.1±53.5 IE; G4= 363.9±190.9 µε. No statistically signifcant difference was found between EH and IH, regardless of the type of copings (p>0.05). The hypotheses were partially accepted. CONCLUSIONS: It was concluded that the type of hexagonal connection and coping presented similar mechanical behavior under tightening conditions.

The aim of this study was to evaluate alternative methods for the disinfection of toothbrushes considering that most of the previously proposed methods are expensive and cannot be easily implemented. Two-hundred toothbrushes with standardized dimensions and bristles were included in the study. The toothbrushes were divided into 20 experimental groups (n = 10), according to microorganism considered and chemical agent used. The toothbrushes were contaminated in vitro by standardized suspensions of Streptococcus mutans, Streptococcus pyogenes, Staphylococcus aureus or Candida albicans. The following disinfectants were tested: 0.12% chlorhexidine digluconate, 50% white vinegar, a triclosan-containing dentifrice solution, and a perborate-based tablet solution. The disinfection method was immersion in the disinfectant for 10 min. After the disinfection procedure, the number of remaining microbial cells was evaluated. The values of cfu/toothbrush of each group of microorganism after disinfection were compared by Kruskal-Wallis ANOVA and Dunn's test for multiple comparisons (5%). The chlorhexidine digluconate solution was the most effective disinfectant. The triclosan-based dentifrice solution promoted a significant reduction of all microorganisms' counts in relation to the control group. As to the disinfection with 50% vinegar, a significant reduction was observed for all the microorganisms, except for C. albicans. The sodium perborate solution was the less effective against the tested microorganisms. Solutions based on triclosan-containing dentifrice may be considered effective, nontoxic, cost-effective, and an easily applicable alternative for the disinfection of toothbrushes. The vinegar solution reduced the presence of S. aureus, S. mutans and S. pyogenes on toothbrushes.

OBJETIVO: Este estudo avaliou a influência da aplicação tópica de flúor fosfato acidulado sobre a rugosidade do esmalte e de diferentes materiais restauradores. METODOLOGIA: Amostras de cimento de ionômero de vidro modificado por resina (Vitremer - V), resina composta microhíbrida com flúor (Tetric Ceram - T) e resina nanopartículada sem flúor em sua composição (Z350 - Z) foram confeccionadas. Metade das amostras recebeu aplicação tópica de flúor fosfato acidulado a 1,23% (FFA) (grupos VF, TF, ZF e EF) e a outra metade não, totalizando 6 grupos experimentais (n=10). Dez terceiros molares humanos hígidos foram utilizados para confecção de amostras de esmalte (E), constituindo os grupos controles (n=10). A rugosidade média superficial (Ra) dos materiais restauradores e do esmalte, com e sem flúor, foi analisada pelo rugosímetro. Os dados foram submetidos à análise de variância, testes de Tukey, t-Student e de Dunn-Bonferroni a 5% de significância. RESULTADOS: Os valores médios de rugosidade, em micrometros, foram: Z=0,60; TF=1,00; ZF=1,05; VF=1,18; T=2,10; V=2,70; E=16,99 e EF=21,19. E e EF apresentaram Ra significativamente superior aos grupos experimentais. CONCLUSÃO: Concluiu-se que o FFA aumentou a rugosidade do esmalte, diminuiu a do cimento de ionômero de vidro e da resina microhíbrida com flúor e não alterou a rugosidade da resina nanoparticulada sem flúor.

PURPOSE: The aim of this study was to assess the influence of topical acidulated phosphate fluoride on the surface roughness of both human enamel and different restorative materials. METHODS: Resin-modified glass ionomer (Vitremer - V), microhybrid composite resin with fluoride (Tetric Ceram - T), and nanofiller composite resin without fluoride (Z350 - Z) were evaluated. Acidulated phosphate fluoride (APF, 1.23%) was applied to half of the specimens (groups VF, TF, ZF and EF). Ten intact human third molars were used to obtain enamel specimens (E). Six experimental groups (n=10) and two control groups (n=10) were formed. The mean surface roughness (Ra) of both the restorative materials and enamel, with and without fluoride, was evaluated with a profilometer. Data were analyzed with an ANOVA, Tukey's test, Student's t-test, and Dunn-Bonferroni test at the 5% significance level. RESULTS: The mean values of surface roughness, in micrometers, were as follows: Z=0.60; TF=1.00; ZF=1.05; VF=1.18; T=2.10; V=2.70; E=16.99; and EF=21.19. Both E and EF presented significantly higher surface roughness than the other experimental groups. CONCLUSION: It was concluded that APF increased the surface roughness of enamel and decreased the surface roughness of both resin-modified glass-ionomer cement and microhybrid composite resin with fluoride. The surface roughness of the nanofiller composite resin was not modified by APF.

OBJECTIVES: To evaluate the microtensile bond strength (µTBS) of one- (Xeno III, Dentsply) and two-step (Tyrian-One Step Plus, Bisco) self-etching adhesive systems bonded to dentin and cemented to chemically cured (C&B Metabond) or light-cured paste of a dual-cure resin cement (Variolink II, Ivoclar) within a short (24 h) and long period of evaluation (90 days). MATERIAL AND METHODS: Forty recently extracted human molars had their roots removed and their occlusal dentin exposed and ground wet with 600-grit SiC paper. After application of one of the adhesives, the resin cement was applied to the bonded surface and a composite resin block was incrementally built up to a height of 5 mm (n=10). The restored teeth were stored in distilled water at 37ºC for 7 days. The teeth were then cut along two axes (x and y), producing beam-shaped specimens with 0.8 mm² cross-sectional area, which were subjected to µTBS testing at a crosshead speed of 0.05 mm/min and stressed to failure after 24 h or 90 days of storage in water. The µTBS data in MPa were subjected to three-way analysis of variance and Tukey's test (α= 0.05). RESULTS: The interaction effect for all three factors was statistically significant (three-way ANOVA, p<0.001). All eight experimental means (MPa) were compared by the Tukey's test (p<0.05) and the following results were obtained: Tyrian-One Step Plus /C&B/24 h (22.4±7.3); Tyrian-One Step Plus /Variolink II/24 h (39.4±11.6); Xeno III/C&B/24 h (40.3±12.9); Xeno III/Variolink II/24 h (25.8±10.5); Tyrian-One Step Plus /C&B/90 d (22.1±12.8) Tyrian-One Step Plus/VariolinkII/90 d (24.2±14.2); Xeno III/C&B/90 d (27.0±13.5); Xeno III/Variolink II/90 d (33.0±8.9). CONCLUSIONS: Xeno III/Variolink II was the luting agent/adhesive combination that provided the most promising bond strength after 90 days of storage in water.

Os bisfosfonatos são empregados atualmente para o tratamento de várias doenças caracterizadas pelo aumento da reabsorção óssea, como a osteoporose. As estatinas são amplamente utilizadas para redução de níveis elevados de colesterol e estudos recentes têm revelado sua ação anabólica no osso. O objetivo deste trabalho foi avaliar a influência da deficiência estrogênica e dos tratamentos com sinvastatina ou alendronato sódico no osso alveolar em ratas. Cinqüenta e quatro ratas sofreram ovariectomia (OVX) ou cirurgia simulada ("sham"). Um mês após, os animais passaram a receber diariamente, via oral, 25 mg/kg de sinvastatina (SIN), 2 mg/kg de alendronato (ALN) ou água (controle). Trinta e cinco dias depois do início do tratamento os animais foram sacrificados, as hemimandíbulas esquerdas removidas e radiografadas em aparelho de raios X digital. Foi calculada a densidade radiográfica em tons de cinza da área de osso alveolar sob o primeiro molar mandibular e os valores foram submetidos a ANOVA, ao nível de 5%. Ratas ovariectomizadas ganharam mais peso (média ± desvio-padrão: 20,06 ± 6,68%) que as demais (12,13 ± 5,63%). Os valores de densidade radiográfica, em tons de cinza, foram menores nos animais do grupo OVX-água (183,49 ± 6,47), significantemente diferentes daqueles observados nos grupos que receberam alendronato ("sham"-ALN: 193,85 ± 3,81; OVX-ALN: 196,06 ± 5,11) e no grupo "sham"-água (193,66 ± 4,36). Outras comparações entre grupos não revelaram diferenças estatísticas. Concluiu-se que a ovariectomia reduziu a densidade óssea alveolar e que o tratamento com alendronato sódico foi eficiente para o tratamento desta situação.

Bisphosphonates are currently used in the treatment of many diseases involving increased bone resorption such as osteoporosis. Statins have been widely used for the treatment of hypercholesterolemia and recent studies have shown that these drugs are also capable of stimulating bone formation. The purpose of this study was to evaluate the influence of an estrogen deficient state and the effects of simvastatin and sodium alendronate therapies on alveolar bone in female rats. Fifty-four rats were either ovariectomized (OVX) or sham operated. A month later, the animals began to receive a daily dose of simvastatin (SIN - 25 mg/kg), sodium alendronate (ALN - 2 mg/kg) or water (control) orally. Thirty-five days after the beginning of the treatment, the rats were sacrificed and their left hemimandibles were removed and radiographed using digital X-ray equipment. The alveolar radiographic density under the first molar was determined with gray-level scaling and the values were submitted to analysis of variance (a = 5%). Ovariectomized rats gained more weight (mean ± standard deviation: 20.06 ± 6.68%) than did the sham operated animals (12.13 ± 5.63%). Alveolar radiographic density values, expressed as gray levels, were lowest in the OVX-water group (183.49 ± 6.47), and differed significantly from those observed for the groups receiving alendronate (sham-ALN: 193.85 ± 3.81; OVX-ALN: 196.06 ± 5.11) and from those of the sham-water group (193.66 ± 4.36). Other comparisons between groups did not show significant differences. It was concluded that the ovariectomy reduced alveolar bone density and that alendronate was efficient for the treatment of this condition.

OBJETIVO: O objetivo deste estudo foi avaliar a reparação óssea em ratos espontaneamente hipertensos (SHR) e compará-la com a de ratos normotensos, avaliando machos e fêmeas. MÉTODOS: Um defeito ósseo foi criado no fêmur esquerdo de 24 SHR (12 machos e 12 fêmeas) e 24 ratos normotensos (12 machos e 12 fêmeas). Os animais foram divididos em dois grupos com diferentes períodos de sacrifício: sete e 21 dias após o procedimento cirúrgico. Após processamento laboratorial de rotina, as análises histológica e histométrica foram realizadas e os dados submetidos à análise de variância (ANOVA) e ao teste de Tukey (5%). RESULTADOS: Machos e fêmeas do mesmo grupo apresentaram características histológicas similares. Após sete dias, todos os animais apresentaram trabéculas ósseas irregulares, entretanto, os osteoblastos periosteais eram achatados nos SHR, enquanto nos normotensos, essas células apresentavam formato cúbico. Após 21 dias, todos os espécimes mostraram fechamento linear em toda a extensão superficial do defeito ósseo e os SHR apresentaram osteoblastos achatados, enquanto os normotensos apresentaram células cúbicas circundando as trabéculas ósseas. A análise estatística dos dados histométricos indicou médias similares entre machos e fêmeas, exceto para ratos normotensos, aos sete dias. Além disso, aos 21 dias, foi observada maior neoformação óssea nos ratos hipertensos quando comparados aos normotensos, tanto machos quanto fêmeas. CONCLUSÃO: Concluiu-se que os animais SHR apresentaram maior neoformação e maturidade óssea nos defeitos que animais normotensos, no período de 21 dias após a cirurgia.

OBJECTIVE: The aim of this study was to evaluate the bone healing in spontaneously hypertensive rats (SHR) and compare the results with normotensive rats, evaluating male and female animals. METHODS: A bone drill defect was created in the left femur of 24 SHR (12 males and 12 females) and 24 normotensive rats (12 males and 12 females). The animals were divided into two groups and sacrificed 7 and 21 days after the surgical procedure. After the routine laboratory processing, histological and histometric analysis were carried out and data were submitted to ANOVA and Tukey’s test (5%). RESULTS: Males and females from the same group had similar histological characteristics. After seven days, all animals presented irregular bone trabeculae. The periosteal osteoblasts were flattened in SHR, and presented a cuboid shape in normotensive animals. After 21 days, the bone defects of all specimens showed a linear closure in all the superficial extension. In addition, SHR presented flattened osteoblasts surrounding the bone trabeculae, while normotensive ones showed cuboidal cells. Statistical analysis of the histometric data indicated similar means between the male and female groups, except for normotensive rats on day 7. In addition, a larger amount of new bone formation was observed in hypertensive when compared to normotensive rats on day 21, in males as well as females. CONCLUSION: We conclude that bone healing in SHR was more significant than in normotensive ones, as shown by the histological and histometric evaluation 21 days after surgery.