O objetivo deste artigo é apresentar as reflexões de um grupo de docentes e pesquisadores, com diferentes formações, perfis e áreas de atuação, sobre o processo de construção de uma nova agenda de pesquisa e extensão interdisciplinares, com vistas à transdisciplinaridade. Para tanto, descreve um processo de concepção e elaboração de projetos, baseado no conceito de pesquisa-ação e tendo a interdisciplinaridade como meta, na caracterização de sistemas socioecológicos complexos. Os projetos realizados enfocaram a resiliência da agricultura familiar, na região de fronteira agrícola intitulado Portal da Amazônia (MT). Discute-se, a partir desse processo, a possibilidade de construção dialógica de projetos, narrando seus principais desafios na busca pela real indissociabilidade entre o ensino, a pesquisa e a extensão. Conclui-se que as escolhas relativas aos fundamentos teóricos e metodológicos que embasaram os projetos foram essenciais para a construção do fazer acadêmico interdisciplinar, e que o processo de pesquisa-ação, conforme construído e aqui descrito, possibilitou a relação dialética entre a transdisciplinaridade e as três dimensões do conhecimento acadêmico, pauta constante nas discussões atuais sobre a avaliação do Ensino Superior no Brasil.

The purpose of this article is to present the reflections of a group of professors and researchers, with different backgrounds, profiles and areas of expertise, on the process of building a new interdisciplinary research and extension agenda, with a view to transdisciplinarity. For that, it describes a process of conception and elaboration of projects, based on the concept of action research and having interdisciplinarity as a goal, in the characterization of complex socio-ecological systems. The projects carried out focused on the resilience of family farming, in the agricultural frontier in the region called ‘Portal da Amazônia’ (MT). From this process, the possibility of dialogical construction of projects is discussed, narrating its main challenges in the search for the real inseparability between teaching, research and extension. The conclusion points out that the choices related to the theoretical and methodological foundations that supported the projects were essential for the construction of interdisciplinary academic practice. The action research process, as constructed and described here, enabled the dialectical relationship between transdisciplinarity and the three dimensions of academic knowledge, constant in current discussions on the evaluation of Higher Education in Brazil.

SUMMARY: Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation.

RESUMEN: Las células mesenquimales (CM) exhiben un gran potencial regenerativo debido a sus propiedades intrínsecas y la capacidad de restaurar la función del tejido, ya sea directamente, a través de la transdiferenciación, o indirectamente, a través de efectos parácrinos. Este estudio tuvo como objetivo evaluar los cambios morfométricos y fenotípicos en CM cultivadas con medio condicionado por nervio facial en presencia o ausencia de factor de crecimiento de fibroblastos 2 (FGF-2). Para el análisis fenotípico cuantitativo, se analizó la expresión de GFAP, OX-42, MAP-2, β-tubulina III, NeuN y NF-200 mediante inmunocitoquímica. Las células cultivadas con medio condicionado por el nervio facial en presencia de FGF-2 expresaban GFAP, OX-42, MAP-2, β-tubulina III, NeuN y NF-200. En promedio, el área y el perímetro de las células positivas para GFAP fueron mayores en el grupo cultivado con medio condicionado por el nervio facial en comparación con el grupo cultivado con medio acondicionado y FGF-2 (p = 0,0001). Este estudio demostró la plasticidad de CM para linajes neuronales y gliales y abre nuevas perspectivas de investigación en terapia celular y transdiferenciación.

Abstract Pasture is the main food source for more than 200 million cattle heads in Brazil. Although Brazilian forage breeding programs have successfully released well-adapted, high-yielding cultivars over the years, the use of genomic tools in these programs is currently limited. These tools are required to tackle the main challenges for tropical forage breeding in Brazil. In this context, this notes lists the main research priorities raised at the workshop “Breeding Forages in the Genomic Era”, which are necessary to accelerate the use of genomic tools for next-generation breeding of tropical forages and allow breeders to increase genetic gains. Additionally, an online discussion forum (hosted at http://www.cnpgl.embrapa.br/genfor) has been launched to strengthen collaborations among research groups. The research priorities and more synergistic collaborations will assist researchers and decision-makers in delivering a sustainable increase in production of animal products, especially beef and milk, which are required to feed a rising world population.

Estudos prévios têm demonstrado que o ácido hialurônico, um glicosaminoglicano que ocorre em doses elevadas e por tempo prolongado na matriz extracelular dos tecidos em cicatrização do feto, é o responsável pelo resultado da cicatrização fetal sem marcas residuais e sem contração. O presente estudo investigou a ação tópica do ácido hialurônico em queimaduras de ratos adultos, até a epitelização completa das lesões. Foram usados 20 ratos Wistar com peso médio 225± 15g, nos quais foi provocada queimadura de 5 cm2 na pele do dorso, sob anestesia com éter sulfúrico. No grupo I (n=10) as queimaduras foram tratadas com aplicação tópica diária de 1ml de ácido hialurônico 1% e no grupo II (n=10) com 1ml de solução salina 0,9%, até a completa epitelização. O tempo de cicatrização foi de 29± 1,33 dias no grupo I e 38± 2,58 no grupo II. A média dos escores histológicos foi de 27,0± 2,78 no grupo I e de 18,1± 3,66 no grupo II. As diferenças mostraram-se significantes (p<0,05). A análise do resultado estético revelou maiores deformidades nas cicatrizes do grupo II (controle). Concluiu-se que o ácido hialurônico tópico contribuiu para acelerar o tempo de cicatrização, melhorou a evolução histológica e o resultado estético em queimaduras do dorso de ratos adultos.

Previous studies have sugested that hyaluronic acid, a naturally occuring glycosaminoglycan in high doses and for a long time on extracelular matrix of healing wounds of fetus, is the responsible for the result of the fetal healing without scar and wound contraction. The present study investigated the topical effect of hyaluronic acid in burns of adult rats, until the complete epitelization of the lesions. Twenty Wistar rats weighting 225±15g were used. A 5 cm2 back burn was done in each rat under anesthesia. In the I group (n=10) the burns were treated with daily topical application of 1ml of 1% hyaluronic acid and in the II group (n=10) the treatment was done with 1ml of saline 0,9%, until the complete healing. The healing time was 29±1,33 days in the I group and 38±2,58 days in the II group . The histologic score was 27,0±2,78 in the I group and 18,1±3,66 in the II group. The differences were significant (p <0,05). The analysis of the aesthetic result revealed larger deformities in the scars of the II group (controls). In conclusion, the topic hyalurinic acid contributed to accelerate the healing rate, it improved the histologic evolution and turned better the aesthetic result in burns of adult rats.

Foram determinados os níveis de metemoglobina em 116 crianças (idade média de 15 meses) do Estado de São Paulo (Brasil). Dois grupos foram formados: Grupo 1, por 92 crianças que consumiam água com teores de nitrato inferiores ao limite de 10 mg N/litro; e Grupo 2, por 24 crianças que bebiam água de poço com níveis de nitratos superiores ao permitido. A metemoglobinemia média do Grupo 1 (0,56%) mostrou-se estatisticamente diferente e inferior (alfa = 0,05) à do Grupo 2 (0,76%).

Methemoglobin levels were determined in the blood samples of 116 children (average age, 15 months) living in the State of S. Paulo (Brazil). Blood from two groups was analyzed: Group 1 was formed by 92 children who drank water with nitrate levels below 10 mg N/liter; whereas Group 2 was formed by 24 children who drank well water with nitrate above the level permitted. The methemoglobin average for Group 1 (0.56%) was statistically different and less that for Group 2 (0.76%).

Foram determinados os níveis de metemoglobina em 116 crianças (idade média de 15 meses) do Estado de São Paulo (Brasil). Dois grupos foram formados: Grupo 1, por 92 crianças que consumiam água com teores de nitrato inferiores ao limite de 10 mg N/litro; e Grupo 2, por 24 crianças que bebiam água de poço com níveis de nitratos superiores ao permitido. A metemoglobinemia média do Grupo 1 (0,56%) mostrou-se estatisticamente diferente e inferior (alfa = 0,05) à do Grupo 2 (0,76%).

Methemoglobin levels were determined in the blood samples of 116 children (average age, 15 months) living in the State of S. Paulo (Brazil). Blood from two groups was analyzed: Group 1 was formed by 92 children who drank water with nitrate levels below 10 mg N/liter; whereas Group 2 was formed by 24 children who drank well water with nitrate above the level permitted. The methemoglobin average for Group 1 (0.56%) was statistically different and less that for Group 2 (0.76%).