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1.
Body fat, cardiovascular risk factors and polymorphism in the FTO gene: randomized clinical trial and different physical exercise for adolescents
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Bila, Wendell C.
; Romano, Márcia C.C.
; Santos, Luciana L. dos
; Silva, Valmin R. da
; Capanema, Flavio D.
; Pfrimer, Karina
; Ferriolli, Eduardo
; Alves, Natália M.C.
; Campos, Cezenário G.
; Carlos, Fabiângelo M.
; Santos, Maria E.S.M. dos
; Lamounier, Joel A.
.
Abstract Objective: To investigate the effects of different physical exercise programs and polymorphisms of the FTO (fat mass and obesity-associated gene) on body composition and cardiovascular risk factors in adolescents with overweight and obesity. Methods: A randomized, parallel, double-blind clinical trial consisting of the adolescent overweight from the state public network, in a simple representative random sample, who participated in an aerobic exercise or weight training intervention for 10 weeks. Anthropometry, body composition, biochemical markers, sexual maturation, and rs9939609 polymorphism in the FTO gene were assessed. 347 adolescents had their characterization of nutritional status. 72 individuals with overweight and obesity were invited to participate. 39 remained for the start of the program and were randomly allocated to both types of intervention. In the end, 26 subjects participated in the intervention programs, with 12 and 14 in the aerobic and weight training programs, respectively. Results: Heterozygous and homozygous bearers of risk allele A participating in the aerobic program showed improvements in glycemia (p = 0.002) and total cholesterol (p = 0.023) and a reduction in body fat mass (p = 0.041). The weight training program reduced glycemia in patients with the risk allele A (p = 0.027). Cameron’s stage four sexual maturation participants were 2.1 times more likely to improve their body fat (CI = 1.31–3.39). Conclusion: Aerobic exercises produced exclusively a significant decrease in fat mass and total cholesterol in patients with risk allele A. Distinct physical exercise programs may cause diverse changes in risk variables related to the health of adolescents.
2.
Deep sea ecosystem exploration and ‘health check’: sampling strategy and methods applied during the iAtlantic_BR10_Petrobras cruise in the Santos Basin, Southwest Atlantic
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Perez, Jose Angel A.
; Barros Neto, Halésio M.C. de
; Arantes, Renata C.M.
; Gaurisas, Daniela Y.
; Silva, Camila F.
; Alves, Fernanda Maria M.
; Costa, Júlia A.
; Eloi, Paula D.C.
; Fonseca, Thayse S.
; Gavazzoni, Lucas
; Lonskis, Isabella da S.
; Nardi, Ricardo U.
; Nascimento, Pedro O.
; Rodrigues, João Vitor M.
; Santos, Ana Lara F. dos
; Santos, Edson C. dos
; Schroeder, Rafael
; Silva, Luis Carlos da
; Souza, Fernanda dos S.S. de
; Bernardino, Ângelo F.
; Cavalcanti, Guarani H.
; Lindner, Alberto
; Mahiques, Michel M. de
; Millo, Christian
; Reis, Priscila
; Sweetman, Andrew K.
; Roberts, J. Murray
.
Abstract The iAtlantic Project has established an international collaborative strategy to improve mapping and characterization of deep and open ocean ecosystems in understudied regions of the Atlantic and evaluate their health. In December 2022, the first iAtlantic expedition in the South Atlantic set off to map and explore seafloor ecosystems in the Santos Basin slope (200-1,000 m depths) in collaboration with the Petrobras ‘Santos Basin - Regional Characterization Project.’ The 17-day ‘iAtlantic_BR10-Petrobras’ cruise was conducted on board the research vessel NPqHOc Vital de Oliveira (Brazilian Navy) and performed (a) water column structure characterization, (b) seafloor morphology mapping, (c) description of benthic habitats and communities by seafloor imagery and biological/ geological sampling, and (d) ex-situ experiments to assess the functioning of sedimentary ecosystems and their responses to climate-related environmental changes. This study describes the rationale behind the iAtlantic_BR10-Petrobras cruise science plan, reports its sampling strategy and methods, and summarizes its collected data and preliminary results.
3.
Where do we aspire to publish? A position paper on scientific communication in biochemistry and molecular biology
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Baptista, M.S.
; Alves, M.J.M.
; Arantes, G.M.
; Armelin, H.A.
; Augusto, O.
; Baldini, R.L.
; Basseres, D.S.
; Bechara, E.J.H.
; Bruni-Cardoso, A.
; Chaimovich, H.
; Colepicolo Neto, P.
; Colli, W.
; Cuccovia, I.M.
; Da-Silva, A.M.
; Di Mascio, P.
; Farah, S.C.
; Ferreira, C.
; Forti, F.L.
; Giordano, R.J.
; Gomes, S.L.
; Gueiros Filho, F.J.
; Hoch, N.C.
; Hotta, C.T.
; Labriola, L.
; Lameu, C.
; Machini, M.T.
; Malnic, B.
; Marana, S.R.
; Medeiros, M.H.G.
; Meotti, F.C.
; Miyamoto, S.
; Oliveira, C.C.
; Souza-Pinto, N.C.
; Reis, E.M.
; Ronsein, G.E.
; Salinas, R.K.
; Schechtman, D.
; Schreier, S.
; Setubal, J.C.
; Sogayar, M.C.
; Souza, G.M.
; Terra, W.R.
; Truzzi, D.R.
; Ulrich, H.
; Verjovski-Almeida, S.
; Winck, F.V.
; Zingales, B.
; Kowaltowski, A.J.
.
Brazilian Journal of Medical and Biological Research
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The scientific publication landscape is changing quickly, with an enormous increase in options and models. Articles can be published in a complex variety of journals that differ in their presentation format (online-only or in-print), editorial organizations that maintain them (commercial and/or society-based), editorial handling (academic or professional editors), editorial board composition (academic or professional), payment options to cover editorial costs (open access or pay-to-read), indexation, visibility, branding, and other aspects. Additionally, online submissions of non-revised versions of manuscripts prior to seeking publication in a peer-reviewed journal (a practice known as pre-printing) are a growing trend in biological sciences. In this changing landscape, researchers in biochemistry and molecular biology must re-think their priorities in terms of scientific output dissemination. The evaluation processes and institutional funding for scientific publications should also be revised accordingly. This article presents the results of discussions within the Department of Biochemistry, University of São Paulo, on this subject.
https://doi.org/10.1590/1414-431x20198935
1769 downloads
4.
Expression of myo-inositol cotransporters in the sciatic nerve and dorsal root ganglia in experimental diabetes
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Farias, V.X.
; Uchoa, P.N.
; Aquino, C.P.
; Britto, L.R.G.
; Fonteles, M.C.
; Leal-Cardoso, J.H.
; Silva-Alves, K.S.
; Havt, A.
; Prata, M.M.G.
; Heimark, D.B.
; Nascimento, N.R.F.
; Santos, C.F.
.
Brazilian Journal of Medical and Biological Research
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The transport of myo-inositol is the main mechanism for the maintenance of its high intracellular levels. We aimed to measure the mRNA and protein levels of myo-inositol cotransporters in the sciatic nerve (SN) and dorsal root ganglia (DRG) during experimental diabetes. Streptozotocin-induced (STZ; 4, 8, and 12 weeks; 65 mg/kg; ip) diabetic rats (DB) and age-matched euglycemic (E) rats were used for the analysis of mRNA and protein levels of sodium myo-inositol cotransporters 1, 2 (SMIT1, SMIT2) or H+/myo-inositol cotransporter (HMIT). There was a significant reduction in the mRNA levels for SMIT1 in the SN and DRG (by 36.9 and 31.0%) in the 4-week DB (DB4) group compared to the E group. SMIT2 was not expressed in SN. The mRNA level for SMIT2 was up-regulated only in the DRG in the DB4 group. On the other hand, the protein level of SMIT1 decreased by 42.5, 41.3, and 44.8% in the SN after 4, 8, and 12 weeks of diabetes, respectively. In addition, there was a decrease of 64.3 and 58.0% of HMIT in membrane and cytosolic fractions, respectively, in the SN of the DB4 group. In the DRG, there was an increase of 230 and 86.3% for SMIT1 and HMIT, respectively, in the DB12 group. The levels of the main inositol transporters, SMIT1 and HMIT, were greatly reduced in the SN but not in the DRG. SMIT-1 was selectively reduced in the sciatic nerve during experimental STZ-induced diabetes.
https://doi.org/10.1590/1414-431x20198589
1015 downloads
5.
Soroprevalência da infecção por Corynebacterium pseudotuberulosis em caprinos no Nordeste brasileiro utilizando técnica de imunoabsorção enzimática (ELISA-indireto)
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Farias, Areano M.
; Alves, José R.A.
; Alves, Francisco S.F.
; Pinheiro, Raimundo R.
; Faccioli-Martins, Patrícia Y.
; Lima, Ana M.C.
; Azevedo, Sérgio S.
; Alves, Clebert J.
.
RESUMO: A caprinocultura na região Nordeste do Brasil desempenha importante função socioeconômica e estratégica. A rusticidade desta atividade nesta região, aliada a sua rápida expansão em outros estados, tem gerado perdas na sua cadeia produtiva a nível regional e nacional, causadas por doenças infecciosas, destacando-se a Linfadenite Caseosa (LC), afecção amplamente difundida nos rebanhos caprinos, acarretando sérios prejuízos econômicos à atividade. Embora LC tenha sido detectada como um problema endêmico no nordeste brasileiro, uma análise abrangente e mais recente desta prevalência nos rebanhos caprinos brasileiros se faz necessária. O objetivo desse estudo foi determinar a frequência de anticorpos anti-Corynebacterium pseudotuberculosis em cinco dos nove estados que compõem a região Nordeste do Brasil. Foram processadas amostras de soro de 2571 caprinos provenientes de 218 propriedades rurais oriundas de cinco estados da região Nordeste do Brasil, coletadas entre os anos de 2010 a 2012. O diagnóstico da infecção por C. pseudotuberculosis foi realizado pela técnica de ELISA-indireto. Em 88,5% (193/218) das propriedades investigadas, pelo menos um caprino foi soropositivo para C. pseudotuberculosis, sugerindo que o agente se encontra disseminado nos rebanhos do Nordeste, com a maior prevalência encontrada no Rio Grande do Norte (94,5%) e a menor no estado de Sergipe (70,3%). Foram identificados 783 (30,4%; IC 95%=28,7-32,2%) caprinos soropositivos, com a maior prevalência entre animais encontrada no Piauí (41,4%) e a menor no estado de Sergipe (22,5%). De um total de 279 reprodutores avaliados, 106 (37,9%) resultaram soropositivos, destacando-se o Rio Grande do Norte, com 45,30%, e Piauí com 46,8% dos reprodutores positivos para LC. Das 1420 matrizes testadas, 599(42,1%) apresentaram sorologia positiva para C. pseudotuberculosis. Entre os estados esta distribuição também se manteve semelhante, destacando-se o Rio Grande do Norte, com 47,5% e Piauí com 59,5% das matrizes positivos para LC. Entre os 872 caprinos jovens avaliados, 78 (8,9%) foram soropositivos para LC, observando-se uma diferença estatística na frequência de sopositividade obtidas entre os adultos e os jovens (P<0,001). Reforça-se a necessidade do diagnóstico da enfermidade em rebanhos caprinos da região Nordeste para possível implementação de programas de controle da doença e medidas mais precisas no manejo da LC junto aos criadores de caprinos.
ABSTRACT: Goat farming in the Northeast region of Brazil plays an important socioeconomic and strategic role. The rusticity of this activity in this region, allied with its rapid expansion in other states, has caused losses in its production chain in regional and national levels, caused by infectious diseases, especially caseous lymphadenitis (CL), a widespread goat herds disease which has been causing serious economic loss to this activity. Although CL has been detected as an endemic problem in the Brazilian Northeast, a comprehensive and more recent analysis of this prevalence in Brazilian goats is necessary. The objective of this study was to determine the frequency of anti-Corynebacterium pseudotuberculosis antibodies in five of nine states of the northeast region of Brazil. Serum samples were collected from 2571 goats from 218 farms in five states in the Northeast region of Brazil, collected between 2010 and 2012. The diagnosis of C. pseudotuberculosis infection was made using the ELISA-indirect technique. In 88.5% (193/218) of the investigated properties, at least one goat was seropositive for C. pseudotuberculosis, suggesting that the agent is widespread in Northeast herds, with the highest prevalence found in Rio Grande do Norte (94.5%) and the lowest one in the state of Sergipe (70.3%). A total of 783 (30.45%; 95% CI = 28.71-32.26%) seropositive goats were found, and the highest prevalence among animals was found in Piauí (41.4%) and the lowest in the state of Sergipe (22.5%). Out of a total of 279 breeding herds, 106 (37.99%) were seropositive, standing out the Rio Grande do Norte, with a total of 45.30%, and Piauí 46.8% of positive breeding for CL. A total of the 1420 tested matrices, 599 (42.1%) presented positive serology for C. pseudotuberculosis. Among the States, this distribution also remained similar, standing out Rio Grande do Norte with 47.5%, and Piauí with 59.5% of positive matrices for CL. Among 872 young goats evaluated, 78 (8.9%) were seropositive for CL, observing a statistical difference in the frequency of soropositivity obtained between adults and young adults (P<0.001). The necessity to diagnose the disease in goat herds in the Northeast region is reinforced for the possible implementation of disease control programs and more precise measures to manage CL along with goat farmers.
https://doi.org/10.1590/1678-5150-pvb-5282
1059 downloads
6.
The genome sequence of Dyella jiangningensis FCAV SCS01 from a lignocellulose-decomposing microbial consortium metagenome reveals potential for biotechnological applications
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Desiderato, Joana G.
; Alvarenga, Danillo O.
; Constancio, Milena T.L.
; Alves, Lucia M.C.
; Varani, Alessandro M.
.
Abstract Cellulose and its associated polymers are structural components of the plant cell wall, constituting one of the major sources of carbon and energy in nature. The carbon cycle is dependent on cellulose- and lignin-decomposing microbial communities and their enzymatic systems acting as consortia. These microbial consortia are under constant exploration for their potential biotechnological use. Herein, we describe the characterization of the genome of Dyella jiangningensis FCAV SCS01, recovered from the metagenome of a lignocellulose-degrading microbial consortium, which was isolated from a sugarcane crop soil under mechanical harvesting and covered by decomposing straw. The 4.7 Mbp genome encodes 4,194 proteins, including 36 glycoside hydrolases (GH), supporting the hypothesis that this bacterium may contribute to lignocellulose decomposition. Comparative analysis among fully sequenced Dyella species indicate that the genome synteny is not conserved, and that D. jiangningensis FCAV SCS01 carries 372 unique genes, including an alpha-glucosidase and maltodextrin glucosidase coding genes, and other potential biomass degradation related genes. Additional genomic features, such as prophage-like, genomic islands and putative new biosynthetic clusters were also uncovered. Overall, D. jiangningensis FCAV SCS01 represents the first South American Dyella genome sequenced and shows an exclusive feature among its genus, related to biomass degradation.
https://doi.org/10.1590/1678-4685-gmb-2017-0155
1038 downloads
7.
Influencia de la actividad física sobre la eliminación urinaria de minerales y elementos traza en sujetos que viven en la misma área geográfica
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Abstract Objective: The aim of this study was to determine the concentrations of macroelements magnesium and phosphorus, and the trace elements arsenic, boron, lithium, cesium, rubidium, tin and strontium in urine of sedentary subjects and long distance athletes, who live in the same area. Method: It was evaluated the urinary concentration of the metals mentioned before. The sample consisted of a group of 21 long-distance athletes. Twenty-six sedentary subjects formed the control group. The procedure consisted of recording anthropometric measurements, heart rate and blood pressure at rest. Then a stress test was performed to determine the maximum heart rate, maximum oxygen consumption, pulmonary ventilation and respiratory exchange ratio. Samples of the first morning urine of all subjects were obtained. Results: Athletes had significantly lower levels (P < .001) of body mass index, body fat (P < .001) and resting heart rate (P < .001). Maximum heart rate and maximum oxygen consumption (P < .001) and pulmonary ventilation (P < .05) were higher in the group of athletes. No differences were observed in urinary concentrations of phosphorus, arsenic, boron, lithium, rubidium and strontium. Urinary concentrations of magnesium (P < .001) and tin (P < .05) were lower in athletes than the control group. However, urinary concentrations of cesium (P < .05) were higher in athletes compared to the control group. Conclusions: Mineral concentrations analyzed are in normal values, so there is no risk to health. Training induces a less urinary excretion for magnesium and tin and an increase in cesium excretion, avoiding the toxicity because of cesium accumulation, without a performance decrease.
Resumo Objetivo: O objetivo deste estudo foi determinar as concentrações do macroelementos magnésio e fósforo, e oligoelementos arsênio, boro, lítio, césio, rubídio, estanho e estrôncio de urina indivíduos sedentários e atletas que vivem na mesma região. Método: Foi avaliada a concentração urinária dos metais acima mencionados. A amostra foi constituída por um grupo de 21 atletas de fundo e um grupo de 26 indivíduos sedentários para grupo controle. Medidas antropométricas, frequência cardíaca e pressão arterial de repouso foram registrados. Eles foram submetidos a um teste de estresse para determinar frequência cardíaca máxima, o consumo máximo de oxigênio, ventilação pulmonar e quociente respiratório. Foram coletados as amostras da primeira urina da manhã de todas os sujeitos. Resultados: Atletas tiveram valores significativamente menores (p < 0.001) no índice de massa corporal, gordura corporal (p < 0.001) e frequência cardíaca em repouso (p < 0.001). A frequência cardíaca máxima, o consumo máximo de oxigênio (p < 0.001) e ventilação pulmonar (p < 0.05) foram maiores no grupo de atletas no grupo de controle. Não houve diferenças significativas em concentrações urinárias de fósforo, arsénio, boro, lítio, rubídio e estrôncio. As concentrações urinárias de magnésio (p < 0.001) e estanho (p < 0.05) foram mais baixas nos atletas do que no grupo de controle. Além disso, as concentrações urinárias de césio (p < 0.05) foram maiores em atletas em comparação com o grupo de controle. Conclusões: As concentrações dos elementos analisados se encontram em intervalos normais, portanto, não há risco para a saúde. A realização de treinamentos sistemáticos provoca uma menor excreção de magnésio, estanho e césio que evita maior toxicidade por acumulo, não prejudicando o desempenho.
Resumen Objetivo: El objetivo de este estudio fue conocer las concentraciones de los macroelementos magnesio y fósforo, y de los elementos traza arsénico, boro, litio, cesio, rubidio, estaño y estroncio, en orina de sujetos sedentarios y deportistas, que viven en la misma región. Método: Se analizó la concentración urinaria de los metales descritos antes. La muestra estaba constituida por un grupo de 21 atletas de fondo y un grupo control formado por 26 sujetos sedentarios. Se registraron medidas antropométricas, la frecuencia cardíaca y la presión arterial en reposo. Fueron sometidos a una prueba de esfuerzo para determinar la frecuencia cardíaca máxima, el consumo máximo de oxígeno, la ventilación pulmonar y el cociente respiratorio. Se tomaron muestras de la primera orina de la mañana de todos los sujetos. Resultados: Los atletas presentaron valores significativamente inferiores (p < 0.001) en índice de masa corporal, grasa corporal (p < 0.001) y frecuencia cardíaca de reposo (p < 0.001). La frecuencia cardíaca máxima, el consumo máximo de oxígeno (p < 0.001) y la ventilación pulmonar (p < 0.05) fueron mayores en el grupo de atletas respecto al grupo control. No existieron diferencias significativas en las concentraciones urinarias de fósforo, arsénico, boro, litio, rubidio y estroncio. Las concentraciones urinarias de magnesio (p < 0.001) y estaño (p < 0.05) fueron menores en los atletas que en el grupo control. Además, las concentraciones urinarias de cesio (p < 0.05) eran mayores en deportistas respecto al grupo control. Conclusiones: Los concentraciones de los elementos analizados se encuentran en rangos normales, no existiendo, por tanto, riesgo para la salud. La realización de entrenamiento sistemático provoca una menor eliminación de magnesio y estaño y mayor de cesio, que evitaría la toxicidad por su acumulación, no perjudicando su rendimiento.
https://doi.org/10.1016/j.ramd.2016.07.003
479 downloads
8.
Administração seriada de salina hipertônica 7,5% na terapia para sepse grave decorrente da síndrome da diarreia hemorrágica aguda em cães
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Barbosa, Breno C.
; Freitas, Patrícia M.C.
; Alves, Fernanda S.
; Salvato, Lauranne A.
; Luz, Marcelo R.
; Paes, Paulo Ricardo O.
; Beier, Suzane L.
; Faleiros, Rafael R.
.
RESUMO: Na clínica de animais de companhia é frequente cães com síndrome da diarreia hemorrágica aguda associada a quadros de sepse, o que acarreta alta mortalidade. Nesse contexto, objetivou-se, em um ensaio clínico controlado aleatorizado de centro único, estudar os efeitos tardios da solução salina hipertônica a 7,5% em aplicações seriadas, sobre variáveis hemodinâmicas, clínicas e laboratoriais em cães com quadro de sepse grave decorrente desta síndrome. Para tal, 12 cães foram aleatoriamente distribuídos em dois grupos de igual número, sendo um controle (CON) e o outro, solução salina hipertônica 7,5% (SSH). Variáveis clínicas e laboratoriais foram avaliadas imediatamente após a admissão do paciente (T0), 24 (T24), 48 (T48) e 72 (T72) horas após a admissão. O grupo SSH recebeu Ringer com lactato, antibioticoterapia, analgésico e SSH 7,5% em bolus (5mL kg-1 em 4 minutos) no T24 e no T48. O grupo CON recebeu a mesma terapia acima, porém ao invés da utilização de SSH a 7,5%, administrou-se bolus de solução de Ringer lactato na mesma dose e tempos utilizado. As avaliações em cada tempo foram realizadas anteriormente à administração dos bolus, nos dois grupos. Avaliaram-se hemograma completo e as variáveis clínicas (escala AVDN, grau de desidratação, frequência respiratória e temperatura retal), frequência cardíaca (FC), pressão arterial sistólica (PAS). Os dados paramétricos foram avaliados pelos testes Student Newman Keuls e teste t de Student, e os não paramétricos pelo teste de Friedman e Mann Whitney, com nível de significância de P≥0,05. Nas variáveis clínicas estudadas não se observou diferença entre os grupos e entre os momentos avaliados. Observou-se diferença significativa no grupo SSH no T72, com elevação da PAS e redução da FC, fato não observado no grupo CON, onde esses parâmetros não se alteraram. O hematócrito e a concentração de hemoglobina diminuíram em ambos os grupos com o tempo. As contagens dos leucócitos totais e dos monócitos apresentaram uma elevação significativa no grupo SSH, estando os leucócitos dentro da faixa de normalidade no T72. Não houve diferenças significativas em relação aos neutrófilos segmentados, porém no grupo SSH verificou-se aumento de 9,5 vezes no T72 comparado com o T24 (P=0,09), enquanto que este aumento foi de apenas 2,5 vezes no grupo CON (P=0,30). Observou-se ainda redução nas contagens de plaquetas e na concentração de globulinas no grupo COM, enquanto essas variáveis se mantiveram estáveis no grupo SSH. Conclui-se que a administração seriada de SSH 7,5% se mostrou promissora no tratamento de cães com síndrome da diarreia hemorrágica aguda, pois auxilia na estabilização dos leucócitos, plaquetas e globulinas de cães com sepse grave decorrente da síndrome da diarreia hemorrágica aguda.
ABSTRACT: The association between acute hemorrhagic diarrhea syndrome and sepsis is frequent in dogs and causes high mortality. In this context we investigated in a randomized single-center controlled trial the late effects of 7.5% hypertonic saline solution in serial applications on hemodynamic, clinical and laboratory variables in dogs with severe sepsis due to the syndrome. Twelve dogs were randomly distributed into two groups of equal numbers, control (CON) and 7.5% hypertonic saline solution (SSH). Clinical and laboratory variables were evaluated immediately after admission of patients (T0), 24 (T24), 48 (T48) and 72 (T72) hours after the admission. The SSH group received Ringer with lactate, antibiotic therapy, analgesic and 5mL/kg-1 bolus of 7.5% SSH over 4 minutes on T24 and T48. The CON group received the same therapy, but instead of 7.5% SSH, Ringer with lactate bolus was given at the same dosis and times. All evaluations were performed prior to the administration of the bolus in both groups. Data collection included complete blood count and clinical variables (AVDN scale, degree of dehydration, respiratory rate and rectal temperature), heart rate (HR) and systolic blood pressure (SBP). Parametric data were evaluated by the Student Newman Keuls and Student t tests, and the nonparametric ones by the Friedman and Mann Whitney test, with a significance level of 0.05. There were no significant differences between the groups and moments in clinical variables. Nevertheless, the SSH group presented a significant elevation of SBP and HR reduction at T72. Hematocrit and hemoglobin concentration decreased in both groups over time. Total leukocyte and monocyte counts showed a significant elevation in the SSH group, however the leukocytes were within the normal range at T72. There were no significant differences in the segmental neutrophils, but a 9.5-fold increase in T72 compared to T24 (P=0.09) in the SSH group was observed, whereas this increase was only 2.5-fold in the CON group (P=0.30). A decrease in platelet counts and globulin concentration was observed in the CON group, while these variables remained stable in the SSH group. In conclusion, serial administration of 7.5% SSH is promising in the therapy of dogs with acute hemorrhagic diarrhea syndrome, since it assists in the stabilization of leukocytes, platelets and globulins in dogs with severe sepsis due to this syndrome.
https://doi.org/10.1590/s0100-736x2017000900011
2130 downloads
9.
Caracterização epidemiológica e fatores de risco associados à infecção por lentivírus de pequenos ruminantes na região do semiárido paraibano, Nordeste do Brasil
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Guilherme, Ricardo F.
; Azevedo, Sérgio S.
; Higino, Severino S.S.
; Alves, Francisco S.F.
; Santiago, Lauana B.
; Lima, Ana M.C.
; Pinheiro, Raymundo R.
; Alves, Clebert J.
.
RESUMO: O objetivo deste estudo foi determinar a soroprevalência de lentivírus de pequenos ruminantes (LVPR) e identificar os fatores de risco para a ocorrência de caprinos e ovinos soropositivos no semiárido do Estado da Paraíba. Foram utilizados 1.733 animais, sendo 1.274 caprinos procedentes de 62 Unidades de Produção (UPs) e 459 ovinos provenientes de 32 UPs. Para o diagnóstico sorológico da infecção por lentivírus foi utilizado o teste de imunodifusão em gel de ágar (IDGA). Dos 1.274 caprinos analisados 15 (1,18%) foram soropositivos, enquanto que todos os 459 ovinos foram soronegativos. Das 62 propriedades caprinas analisadas oito (12,9%) apresentaram pelo menos um animal soropositivo. Os fatores de risco para a ocorrência de caprinos soropositivos foram área da propriedade (odds ratio = 3,28; p = 0,044), ausência de capacitação dos produtores (odds ratio = 8,29; p = 0,042) e uso de monta natural não controlada (odds ratio = 6,78; p = 0,012). Conclui-se que a infecção por lentivírus de pequenos ruminantes, demonstrada pela detecção de anticorpos, está disseminada em rebanhos caprinos do semiárido paraibano, e sugere-se o incentivo à capacitação contínua dos produtores, manutenção de reprodutores negativos ao LVPR e utilização de inseminação artificial com o intuito de evitar o contato físico entre macho e fêmeas.
ABSTRACT: The aim of this survey was to determine the seroprevalence of small ruminant lentivirus (SRLV) and to identify risk factors for the occurrence of seropositive goats and sheep in the semiarid region of Paraiba State. It were used 1,733 animals, being 1,274 goats from 62 Production Units (PU) and 459 sheep from 38 PU. For the serological diagnosis of lentivirus infection the agar gel immunodiffusion test (AGID) was used. Of the 1,274 goats 15 (1.18%) were seropositive, and all 459 sheep were seronegative. Of the 62 goat herds eight (12.9%) presented at least one seropositive animal. Risk factors for the occurrence of seropositive goats were area of the property ≤35 ha (odds ratio = 3.28; p=0.044), not training of producers (odds ratio = 8.29; p=0.042) and use of uncontrolled natural mating (odds ratio = 6.78; p=0.012). It is concluded that lentivirus infection detected by serology is spread in goat flocks in the semiarid of the State of Paraíba, and it is suggested to encourage the continous capacitation of owners, maintenance of reproducers negative for SRLV and use of artificial insemination aiming to avoid the physical contact among male and females.
https://doi.org/10.1590/s0100-736x2017000600002
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10.
Goat umbilical cord cells are permissive to small ruminant lentivirus infection in vitro
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Martins, Gabrielle R.
; Marinho, Rebeca C.
; Bezerra Junior, Rosivaldo Q.
; Alves, Antoniel de O.
; Câmara, Lilia M.C.
; Albuquerque-Pinto, Luiz C.
; Teixeira, Maria F. da S.
.
Abstract Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture. To accomplish this goal, cells were isolated from umbilical cords; characterized phenotypically by flow cytometry analysis; differentiate into osteogenic, chondrogenic and adipogenic lineage; and submitted to viral challenge. The proliferation of goat umbilical cord cells was fast and cell monolayers formed after 15 days. These cells exhibited morphology, immunophenotype, growth characteristics, and lineage differentiation potential similar to mesenchymal stem cells of other origins. The goat umbilical cord derived cells stained positive for vimentin and CD90, but negative for cytokeratin, CD34 and CD105 markers. Syncytia and cell lysis were observed in cell monolayers infected by CAEV-Cork and MVV-K1514, showing that the cells are permissive to small ruminant lentivirus infection in vitro. These data demonstrate the proliferative competence of cells derived from goat umbilical cords and provide a sound basis for future research to standardize this cell lineage.
https://doi.org/10.1016/j.bjm.2016.11.002
1029 downloads
11.
Growing knowledge: an overview of Seed Plant diversity in Brazil
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Zappi, Daniela C.
; Filardi, Fabiana L. Ranzato
; Leitman, Paula
; Souza, Vinícius C.
; Walter, Bruno M.T.
; Pirani, José R.
; Morim, Marli P.
; Queiroz, Luciano P.
; Cavalcanti, Taciana B.
; Mansano, Vidal F.
; Forzza, Rafaela C.
; Abreu, Maria C.
; Acevedo-Rodríguez, Pedro
; Agra, Maria F.
; Almeida Jr., Eduardo B.
; Almeida, Gracineide S.S.
; Almeida, Rafael F.
; Alves, Flávio M.
; Alves, Marccus
; Alves-Araujo, Anderson
; Amaral, Maria C.E.
; Amorim, André M.
; Amorim, Bruno
; Andrade, Ivanilza M.
; Andreata, Regina H.P.
; Andrino, Caroline O.
; Anunciação, Elisete A.
; Aona, Lidyanne Y.S.
; Aranguren, Yani
; Aranha Filho, João L.M.
; Araújo, Andrea O.
; Araújo, Ariclenes A.M.
; Araújo, Diogo
; Arbo, María M.
; Assis, Leandro
; Assis, Marta C.
; Assunção, Vivian A.
; Athiê-Souza, Sarah M.
; Azevedo, Cecilia O.
; Baitello, João B.
; Barberena, Felipe F.V.A.
; Barbosa, Maria R.V.
; Barros, Fábio
; Barros, Lucas A.V.
; Barros, Michel J.F.
; Baumgratz, José F.A.
; Bernacci, Luis C.
; Berry, Paul E.
; Bigio, Narcísio C.
; Biral, Leonardo
; Bittrich, Volker
; Borges, Rafael A.X.
; Bortoluzzi, Roseli L.C.
; Bove, Cláudia P.
; Bovini, Massimo G.
; Braga, João M.A.
; Braz, Denise M.
; Bringel Jr., João B.A.
; Bruniera, Carla P.
; Buturi, Camila V.
; Cabral, Elza
; Cabral, Fernanda N.
; Caddah, Mayara K.
; Caires, Claudenir S.
; Calazans, Luana S.B.
; Calió, Maria F.
; Camargo, Rodrigo A.
; Campbell, Lisa
; Canto-Dorow, Thais S.
; Carauta, Jorge P.P.
; Cardiel, José M.
; Cardoso, Domingos B.O.S.
; Cardoso, Leandro J.T.
; Carneiro, Camila R.
; Carneiro, Cláudia E.
; Carneiro-Torres, Daniela S.
; Carrijo, Tatiana T.
; Caruzo, Maria B.R.
; Carvalho, Maria L.S.
; Carvalho-Silva, Micheline
; Castello, Ana C.D.
; Cavalheiro, Larissa
; Cervi, Armando C.
; Chacon, Roberta G.
; Chautems, Alain
; Chiavegatto, Berenice
; Chukr, Nádia S.
; Coelho, Alexa A.O.P.
; Coelho, Marcus A.N.
; Coelho, Rubens L.G.
; Cordeiro, Inês
; Cordula, Elizabeth
; Cornejo, Xavier
; Côrtes, Ana L.A.
; Costa, Andrea F.
; Costa, Fabiane N.
; Costa, Jorge A.S.
; Costa, Leila C.
; Costa-e-Silva, Maria B.
; Costa-Lima, James L.
; Cota, Maria R.C.
; Couto, Ricardo S.
; Daly, Douglas C.
; De Stefano, Rodrigo D.
; De Toni, Karen
; Dematteis, Massimiliano
; Dettke, Greta A.
; Di Maio, Fernando R.
; Dórea, Marcos C.
; Duarte, Marília C.
; Dutilh, Julie H.A.
; Dutra, Valquíria F.
; Echternacht, Lívia
; Eggers, Lilian
; Esteves, Gerleni
; Ezcurra, Cecilia
; Falcão Junior, Marcus J.A.
; Feres, Fabíola
; Fernandes, José M.
; Ferreira, D.M.C.
; Ferreira, Fabrício M.
; Ferreira, Gabriel E.
; Ferreira, Priscila P.A.
; Ferreira, Silvana C.
; Ferrucci, Maria S.
; Fiaschi, Pedro
; Filgueiras, Tarciso S.
; Firens, Marcela
; Flores, Andreia S.
; Forero, Enrique
; Forster, Wellington
; Fortuna-Perez, Ana P.
; Fortunato, Reneé H.
; Fraga, Cléudio N.
; França, Flávio
; Francener, Augusto
; Freitas, Joelcio
; Freitas, Maria F.
; Fritsch, Peter W.
; Furtado, Samyra G.
; Gaglioti, André L.
; Garcia, Flávia C.P.
; Germano Filho, Pedro
; Giacomin, Leandro
; Gil, André S.B.
; Giulietti, Ana M.
; A.P.Godoy, Silvana
; Goldenberg, Renato
; Gomes da Costa, Géssica A.
; Gomes, Mário
; Gomes-Klein, Vera L.
; Gonçalves, Eduardo Gomes
; Graham, Shirley
; Groppo, Milton
; Guedes, Juliana S.
; Guimarães, Leonardo R.S.
; Guimarães, Paulo J.F.
; Guimarães, Elsie F.
; Gutierrez, Raul
; Harley, Raymond
; Hassemer, Gustavo
; Hattori, Eric K.O.
; Hefler, Sonia M.
; Heiden, Gustavo
; Henderson, Andrew
; Hensold, Nancy
; Hiepko, Paul
; Holanda, Ana S.S.
; Iganci, João R.V.
; Imig, Daniela C.
; Indriunas, Alexandre
; Jacques, Eliane L.
; Jardim, Jomar G.
; Kamer, Hiltje M.
; Kameyama, Cíntia
; Kinoshita, Luiza S.
; Kirizawa, Mizué
; Klitgaard, Bente B.
; Koch, Ingrid
; Koschnitzke, Cristiana
; Krauss, Nathália P.
; Kriebel, Ricardo
; Kuntz, Juliana
; Larocca, João
; Leal, Eduardo S.
; Lewis, Gwilym P.
; Lima, Carla T.
; Lima, Haroldo C.
; Lima, Itamar B.
; Lima, Laíce F.G.
; Lima, Laura C.P.
; Lima, Leticia R.
; Lima, Luís F.P.
; Lima, Rita B.
; Lírio, Elton J.
; Liro, Renata M.
; Lleras, Eduardo
; Lobão, Adriana
; Loeuille, Benoit
; Lohmann, Lúcia G.
; Loiola, Maria I.B.
; Lombardi, Julio A.
; Longhi-Wagner, Hilda M.
; Lopes, Rosana C.
; Lorencini, Tiago S.
; Louzada, Rafael B.
; Lovo, Juliana
; Lozano, Eduardo D.
; Lucas, Eve
; Ludtke, Raquel
; Luz, Christian L.
; Maas, Paul
; Machado, Anderson F.P.
; Macias, Leila
; Maciel, Jefferson R.
; Magenta, Mara A.G.
; Mamede, Maria C.H.
; Manoel, Evelin A.
; Marchioretto, Maria S.
; Marques, Juliana S.
; Marquete, Nilda
; Marquete, Ronaldo
; Martinelli, Gustavo
; Martins da Silva, Regina C.V.
; Martins, Ângela B.
; Martins, Erika R.
; Martins, Márcio L.L.
; Martins, Milena V.
; Martins, Renata C.
; Matias, Ligia Q.
; Maya-L., Carlos A.
; Mayo, Simon
; Mazine, Fiorella
; Medeiros, Debora
; Medeiros, Erika S.
; Medeiros, Herison
; Medeiros, João D.
; Meireles, José E.
; Mello-Silva, Renato
; Melo, Aline
; Melo, André L.
; Melo, Efigênia
; Melo, José I.M.
; Menezes, Cristine G.
; Menini Neto, Luiz
; Mentz, Lilian A.
; Mezzonato, A.C.
; Michelangeli, Fabián A.
; Milward-de-Azevedo, Michaele A.
; Miotto, Silvia T.S.
; Miranda, Vitor F.O.
; Mondin, Cláudio A.
; Monge, Marcelo
; Monteiro, Daniele
; Monteiro, Raquel F.
; Moraes, Marta D.
; Moraes, Pedro L.R.
; Mori, Scott A.
; Mota, Aline C.
; Mota, Nara F.O.
; Moura, Tania M.
; Mulgura, Maria
; Nakajima, Jimi N.
; Nardy, Camila
; Nascimento Júnior, José E.
; Noblick, Larry
; Nunes, Teonildes S.
; O'Leary, Nataly
; Oliveira, Arline S.
; Oliveira, Caetano T.
; Oliveira, Juliana A.
; Oliveira, Luciana S.D.
; Oliveira, Maria L.A.A.
; Oliveira, Regina C.
; Oliveira, Renata S.
; Oliveira, Reyjane P.
; Paixão-Souza, Bruno
; Parra, Lara R.
; Pasini, Eduardo
; Pastore, José F.B.
; Pastore, Mayara
; Paula-Souza, Juliana
; Pederneiras, Leandro C.
; Peixoto, Ariane L.
; Pelissari, Gisela
; Pellegrini, Marco O.O.
; Pennington, Toby
; Perdiz, Ricardo O.
; Pereira, Anna C.M.
; Pereira, Maria S.
; Pereira, Rodrigo A.S.
; Pessoa, Clenia
; Pessoa, Edlley M.
; Pessoa, Maria C.R.
; Pinto, Luiz J.S.
; Pinto, Rafael B.
; Pontes, Tiago A.
; Prance, Ghillean T.
; Proença, Carolyn
; Profice, Sheila R.
; Pscheidt, Allan C.
; Queiroz, George A.
; Queiroz, Rubens T.
; Quinet, Alexandre
; Rainer, Heimo
; Ramos, Eliana
; Rando, Juliana G.
; Rapini, Alessandro
; Reginato, Marcelo
; Reis, Ilka P.
; Reis, Priscila A.
; Ribeiro, André R.O.
; Ribeiro, José E.L.S.
; Riina, Ricarda
; Ritter, Mara R.
; Rivadavia, Fernando
; Rocha, Antônio E.S.
; Rocha, Maria J.R.
; Rodrigues, Izabella M.C.
; Rodrigues, Karina F.
; Rodrigues, Rodrigo S.
; Rodrigues, Rodrigo S.
; Rodrigues, Vinícius T.
; Rodrigues, William
; Romaniuc Neto, Sérgio
; Romão, Gerson O.
; Romero, Rosana
; Roque, Nádia
; Rosa, Patrícia
; Rossi, Lúcia
; Sá, Cyl F.C.
; Saavedra, Mariana M.
; Saka, Mariana
; Sakuragui, Cássia M.
; Salas, Roberto M.
; Sales, Margareth F.
; Salimena, Fatima R.G.
; Sampaio, Daniela
; Sancho, Gisela
; Sano, Paulo T.
; Santos, Alessandra
; Santos, Élide P.
; Santos, Juliana S.
; Santos, Marianna R.
; Santos-Gonçalves, Ana P.
; Santos-Silva, Fernanda
; São-Mateus, Wallace
; Saraiva, Deisy P.
; Saridakis, Dennis P.
; Sartori, Ângela L.B.
; Scalon, Viviane R.
; Schneider, Ângelo
; Sebastiani, Renata
; Secco, Ricardo S.
; Senna, Luisa
; Senna-Valle, Luci
; Shirasuna, Regina T.
; Silva Filho, Pedro J.S.
; Silva, Anádria S.
; Silva, Christian
; Silva, Genilson A.R.
; Silva, Gisele O.
; Silva, Márcia C.R.
; Silva, Marcos J.
; Silva, Marcos J.
; Silva, Otávio L.M.
; Silva, Rafaela A.P.
; Silva, Saura R.
; Silva, Tania R.S.
; Silva-Gonçalves, Kelly C.
; Silva-Luz, Cíntia L.
; Simão-Bianchini, Rosângela
; Simões, André O.
; Simpson, Beryl
; Siniscalchi, Carolina M.
; Siqueira Filho, José A.
; Siqueira, Carlos E.
; Siqueira, Josafá C.
; Smith, Nathan P.
; Snak, Cristiane
; Soares Neto, Raimundo L.
; Soares, Kelen P.
; Soares, Marcos V.B.
; Soares, Maria L.
; Soares, Polyana N.
; Sobral, Marcos
; Sodré, Rodolfo C.
; Somner, Genise V.
; Sothers, Cynthia A.
; Sousa, Danilo J.L.
; Souza, Elnatan B.
; Souza, Élvia R.
; Souza, Marcelo
; Souza, Maria L.D.R.
; Souza-Buturi, Fátima O.
; Spina, Andréa P.
; Stapf, María N.S.
; Stefano, Marina V.
; Stehmann, João R.
; Steinmann, Victor
; Takeuchi, Cátia
; Taylor, Charlotte M.
; Taylor, Nigel P.
; Teles, Aristônio M.
; Temponi, Lívia G.
; Terra-Araujo, Mário H.
; Thode, Veronica
; Thomas, W.Wayt
; Tissot-Squalli, Mara L.
; Torke, Benjamin M.
; Torres, Roseli B.
; Tozzi, Ana M.G.A.
; Trad, Rafaela J.
; Trevisan, Rafael
; Trovó, Marcelo
; Valls, José F.M.
; Vaz, Angela M.S.F.
; Versieux, Leonardo
; Viana, Pedro L.
; Vianna Filho, Marcelo D.M.
; Vieira, Ana O.S.
; Vieira, Diego D.
; Vignoli-Silva, Márcia
; Vilar, Thaisa
; Vinhos, Franklin
; Wallnöfer, Bruno
; Wanderley, Maria G.L.
; Wasshausen, Dieter
; Watanabe, Maurício T.C.
; Weigend, Maximilian
; Welker, Cassiano A.D.
; Woodgyer, Elizabeth
; Xifreda, Cecilia C.
; Yamamoto, Kikyo
; Zanin, Ana
; Zenni, Rafael D.
; Zickel, Carmem S
.
Resumo Um levantamento atualizado das plantas com sementes e análises relevantes acerca desta biodiversidade são apresentados. Este trabalho se iniciou em 2010 com a publicação do Catálogo de Plantas e Fungos e, desde então vem sendo atualizado por mais de 430 especialistas trabalhando online. O Brasil abriga atualmente 32.086 espécies nativas de Angiospermas e 23 espécies nativas de Gimnospermas e estes novos dados mostram um aumento de 3% da riqueza em relação a 2010. A Amazônia é o Domínio Fitogeográfico com o maior número de espécies de Gimnospermas, enquanto que a Floresta Atlântica possui a maior riqueza de Angiospermas. Houve um crescimento considerável no número de espécies e nas taxas de endemismo para a maioria dos Domínios (Caatinga, Cerrado, Floresta Atlântica, Pampa e Pantanal), com exceção da Amazônia que apresentou uma diminuição de 2,5% de endemicidade. Entretanto, a maior parte das plantas com sementes que ocorrem no Brasil (57,4%) é endêmica deste território. A proporção de formas de vida varia de acordo com os diferentes Domínios: árvores são mais expressivas na Amazônia e Floresta Atlântica do que nos outros biomas, ervas são dominantes no Pampa e as lianas apresentam riqueza expressiva na Amazônia, Floresta Atlântica e Pantanal. Este trabalho não só quantifica a biodiversidade brasileira, mas também indica as lacunas de conhecimento e o desafio a ser enfrentado para a conservação desta flora.
Abstract An updated inventory of Brazilian seed plants is presented and offers important insights into the country's biodiversity. This work started in 2010, with the publication of the Plants and Fungi Catalogue, and has been updated since by more than 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing an increase of 3% in its species richness in relation to 2010. The Amazon Rainforest is the richest Brazilian biome for Gymnosperms, while the Atlantic Rainforest is the richest one for Angiosperms. There was a considerable increment in the number of species and endemism rates for biomes, except for the Amazon that showed a decrease of 2.5% of recorded endemics. However, well over half of Brazillian seed plant species (57.4%) is endemic to this territory. The proportion of life-forms varies among different biomes: trees are more expressive in the Amazon and Atlantic Rainforest biomes while herbs predominate in the Pampa, and lianas are more expressive in the Amazon, Atlantic Rainforest, and Pantanal. This compilation serves not only to quantify Brazilian biodiversity, but also to highlight areas where there information is lacking and to provide a framework for the challenge faced in conserving Brazil's unique and diverse flora.
https://doi.org/10.1590/2175-7860201566411
33340 downloads
12.
Comparação entre métodos para determinar o período anterior à interferência de plantas daninhas em feijoeiros com distintos tipos de hábitos de crescimento
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A interferência causada pelas plantas daninhas pode reduzir em até 80% a produtividade de grãos do feijão quando não manejadas de forma correta. Portanto, a decisão sobre o momento de controlá-las é um dos principais aspectos do manejo integrado. Nesse sentido, objetivou-se com este trabalho determinar o período anterior à interferência das plantas daninhas (PAI) em cultivares de feijão com diferentes tipos de hábitos de crescimento. Adotou-se o delineamento experimental de blocos casualizados com quatro repetições. Os tratamentos experimentais foram constituídos de dez períodos de convivência da cultura com as plantas daninhas: 0-7, 0-14, 0-21, 0-28, 0-35, 0-42, 0-49, 0-56, 0-97 (colheita) dias após a emergência (DAE) e mais uma testemunha sem convívio com as plantas daninhas. O PAI foi determinado por meio de distintas abordagens: o nível arbitrário de 5% de perda na produtividade, o nível de tolerância (NT) e o de dano no rendimento econômico (PADRE). O PAI obtido foi diferente em todas as abordagens, e os feijoeiros de crescimento indeterminado tipo II apresentaram os menores tempos de convivência.
Beans grain productivity may be reduced by up to 80% and, therefore, knowing the proper time to control weeds becomes fundamental, his research was conducted in order to determine the period prior to interference (PPI) on bean cultivars of different types' plant growth through distinct approaches: arbitrary level of 5% productivity loss; tolerance level (TL), and the Weed Period Prior to Economic Loss (WEEPPEL). Experimental treatments were constituted of ten periods of coexistence, periods of the crop with the weeds: 0-7; 0-14; 0-21; 0-28; 0-35; 0-42; 0-49;0-56; 0-97 (harvesting) days after emergency (DAE), and a control plot, weed free for the entire period. The experimental design was of the Random Block Design, with four replications. PPI was obtained in all of the different approaches, and the bean plant type II with indeterminate growth had the lowest times of coexistence.
2164 downloads
13.
Supressão de plantas daninhas utilizando plantas de cobertura do solo
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Este trabalho foi realizado com o objetivo de avaliar a cobertura do solo e o efeito supressivo sobre plantas daninhas utilizando plantas de cobertura, em diferentes densidades de semeadura. Os experimentos foram instalados em Votuporanga-SP e Selvíria-MS, em março de 2008, após o preparo convencional do solo. O delineamento experimental utilizado foi o de blocos casualizados com quatro repetições, onde as plantas de cobertura com diferentes densidades de semeadura constituíram os tratamentos: Sorghum bicolor : 6, 7 e 8 kg ha-1; Pennisetum americanum: 10, 15 e 20 kg ha-1; Sorghum sudanense: 12, 15 e 18 kg ha-1; híbrido de S. bicolor com S. sudanense: 8, 9 e 10 kg ha-1; Urochloa ruziziensis: 8, 12 e 16 kg ha-1; e um tratamento controle com vegetação espontânea. Após o manejo das coberturas, foi semeada a soja. Avaliou-se a biomassa seca e densidade das plantas daninhas no momento do corte/colheita das plantas de cobertura. Em Votuporanga, também foi feita uma avaliação das plantas daninhas aos 35 dias após a semeadura da soja. A cobertura do solo proporcionada pelas coberturas foi avaliada no momento da dessecação e no florescimento da cultura da soja. Concluiu-se que U. ruziziensis e S. sudanense reduziram a infestação das plantas daninhas em mais de 90% e mantiveram a cobertura do solo superior a 80% até o florescimento da cultura da soja.
This study aimed to evaluate soil cover and the suppressive effect on weeds by different cover crops at different seed densities. The experiments were set up in Votuporanga, state of São Paulo, Brazil and in Selvíria, state of Mato Grosso do Sul, Brazil, in March 2008, after conventional tillage. The experimental design was a randomized complete block with four replications, using the following cover crops at different seed densities per hectare: Sorghum bicolor : 6, 7 and 8kgha-1, Pennisetum americanum, 10, 15 and 20 kg ha-1, Sorghum sudanense, 12, 15 and 18kgha-1, hybrid of Sorghum bicolor with Sorghum sudanense: 8, 9 and 10kgha-1, Urochloa ruziziensis: 8, 12 and 16kgha-1. A control treatment with spontaneous vegetation was used. Soybean was sown after the management of cover crops. Dry matter and weed density were evaluated at cutting/harvesting of cover crops. In Votuporanga, another assessment of weed plants was made at 35 days after soybean had been sown. The ground cover provided by cover crops was assessed at the time of desiccation and flowering of soybeans. It was concluded that U. ruziziensis and S. sudanense reduced weed infestations by more than 90% and kept ground cover above 80% by the time of flowering of soybean.
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14.
Interferencia de malezas en el cultivo de frijol en dos sistemas de labranzas
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The research aim to determine the period prior to weed interference on common beans (Phaseolus vulgaris). The treatments had been made out of seven periods of coexisting by the culture with the weed 0-10, 0-20, 0-30, 0-40, 0-50, 0-60, and 0 70 days after crop emergence in absence and presence crop residue. It had reduction of 40.56% and 55.12%, the productivity of grains in the culture of the common bean when the coexistence with the weed was during all the cycle of the culture, in absence and presence crop residue, respectively. It was concluded that grain yield was negatively affected from the 29th and 38th days after emergence, in the absence and presence of crop residue, respectively.
Con este trabajo, se busco determinar el período anterior a la interferencia de las malezas que conviven con el cultivo de frijol, en ausencia o presencia de residuos vegetales de Crotalaria juncea. Los tratamientos estaban constituidos de siete períodos de convivencia del cultivo con las malezas: 0-10, 0-20, 0-30, 0-40, 0-50, 0-60 y 0-70 días después de la emergencia y también de un testigo sin presencia de las malezas en ausencia o presencia de crotalaria. El diseño experimental utilizado fue de bloques completos seleccionados al azar, con cuatro repeticiones por tratamiento. Hubo reducciones de 40,6% y 55,1% en la productividad de granos de frijol en convivencia con malezas durante todo el ciclo del cultivo, en ausencia y presencia de los residuos vegetales, respectivamente. Se concluyó que la productividad de granos fue afectada negativamente a partir de 29 y 38 días después de la emergencia, sin y con residuos, respectivamente, y eso constituyó los períodos anteriores a la interferencia.
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Cited 2 times in SciELO
15.
I Diretriz sobre o consumo de gorduras e saúde cardiovascular
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Santos, R.D.
; Gagliardi, A.C.M.
; Xavier, H.T.
; Magnoni, C.D.
; Cassani, R .
; Lottenberg, A.M.P.
; Casella Filho, A.
; Araújo, D.B.
; Cesena, F.Y.
; Alves, R.J.
; Fenelon, G.
; Nishioka, S.A.D.
; Faludi, A.A.
; Geloneze, B.
; Scherr, C.
; Kovacs, C.
; Tomazzela, C.
; Carla, C.
; Barrera-Arellano, D.
; Cintra, D.
; Quintão, E.
; Nakandakare, E.R.
; Fonseca, F.A.H.
; Pimentel, I.
; Santos, J.E.
; Bertolami, M.C.
; Rogero, M.
; Izar, M.C.
; Nakasato, M.
; Damasceno, N.R.T.
; Maranhão, R.
; Cassani, R.S.L.
; Perim, R.
; Ramos, S.
.
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