Abstract Hebanthe eriantha (Martius) Kuntze and Pfaffia glomerata (Spreng) Pedersen are medicinal plants popularly known as “Brazilian Ginseng” due to their similarity to Panax ginseng. In Brazil, they are sold as the same herb, despite their different pharmacological and toxicological properties. The morphological identification is difficult, which facilitates their adulteration. We report the application of the Barcode DNA High-Resolution Melting (Bar-HRM) using matK gene to differentiate both species in samples sold in the Brazilian market. Using the proposed method, we could discriminate and identify both species. Bar-HRM analysis allowed discriminating and identifying both species. It allowed the identification of H. eriantha and P. glomerata in 43.6% and 56.4% of the amplified samples, respectively. Of these, only seven samples were authenticated and, in 71.4% of the cases, adulterated. We concluded that Bar-HRM has proven to be a fast alternative method to authenticate plants under the common name “Brazilian Ginseng”.

Introduction: Globally, depression rates are high among men who have sex with men (MSM). Multiple factors may interact synergistically to increase this risk. This analysis assessed the prevalence of symptoms of depression among MSM in Brazil and synergistic effects of several factors. Methods: Cross-sectional study conducted in 12 cities using respondent-driven sampling. Sociodemographic and behavioral characteristics were collected. The PHQ-9 was used to screen for depression. Having moderate-severe depressive symptoms was compared to none-mild using logistic regression. The syndemic factor was a composite of hazardous alcohol use, sexual violence, and discrimination due to sexual orientation. Those with one to three of these factors were compared to those with none. Results: The weighted prevalence of moderate-severe depressive symptoms was 24.9% (95%CI = 21.8-28.8) and 16.2%, 22.9%, 46.0% and 51.0% when none, one, two, or three syndemic factors were present, respectively, indicating a dose-response effect. Perception of HIV risk, high level of HIV knowledge, known HIV infection, and health self-rated as poor or very poor were also associated with depressive symptoms. Conclusion: The prevalence of moderate-severe depressive symptoms among MSM in Brazil is high, and selected factors act synergistically in increasing their prevalence. Public health policies should consider holistic depression prevention and treatment interventions for this population.

RESUMO O Coronavírus possui ácido ribonucléico de fita simples, sendo o sétimo membro conhecido da família Coronaviridae que infectam humanos. As gotículas e aerossóis que ficam suspensas no ar com o vírus pode ser transmitido por contato como mucosas bucal, nasais e oculares, podendo causar a síndrome respiratória aguda grave. Devido ao surto da nova doença do coronavírus, tornou-se necessário a utilização constante dos equipamentos de proteção individual pelos profissionais de saúde, visto que o vírus possui disseminação rápida e generalizada. Mas, inesperadamente o surto do coronavírus trouxe outra preocupação, o surgimento de lesões faciais pelo uso contínuo e inadequado destes protetores individuais, prejudicando a saúde desses profissionais. Uma alternativa para amenizar e prevenir tais lesões seria a utilização de cremes e curativos profiláticos nos locais onde há pressão causada por estes equipamentos, assim como o alivio das cargas geradas pelos mesmos, em intervalos que não ultrapassem 4 horas. Diante disso, esta revisão de literatura objetivou relatar o potencial de surgimento de lesões faciais causado pelo uso continuo e inadequado dos EPI’s, evidenciando as áreas mais acometidas, os riscos à saúde do profissional, a prevenção e o tratamento, através da consulta eletrônica à base de dados, PUBMED, SCOPUS e Bireme/BVS.

ABSTRACT The single-stranded ribonucleic acid coronavirus is the seventh known member of the Coronaviridae family to infect humans. The droplets and aerosols that are suspended in the air with the virus can be transmitted by contact with the oral, nasal and eye mucosa, which can cause severe acute respiratory syndrome. Due to the outbreak of the new coronavirus disease, the constant use of personal protective equipment by health professionals has become necessary, as the virus has a rapid and widespread spread. But, unexpectedly, the coronavirus outbreak brought another concern, the emergence of facial injuries due to the continuous and inappropriate use of these individual protectors, harming the health of these professionals. An alternative to alleviate and prevent such injuries would be the use of prophylactic creams and dressings in places where there is pressure caused by these equipment, as well as relieving the loads generated by them, at intervals that do not exceed 4 hours. Therefore, this literature review aimed to report the potential for the emergence of facial injuries caused by the continuous and inappropriate use of PPE, highlighting the most affected areas, the risks to the health of the professional, prevention and treatment, through the electronic consultation based on of data, PUBMED, SCOPUS and Bireme/BVS.

RESUMO A rápida disseminação da COVID-19 ao redor do mundo gerou significativos impactos no modo de viver das sociedades, sendo um deles, a escassez de equipamentos de proteção individual (EPIs) para trabalhadores que atuam na linha de frente no combate ao vírus. A insegurança sanitária levou populações e profissionais da saúde a uma busca desenfreada por equipamentos de proteção, além de outros aparatos necessários à manutenção da vida, tanto dos pacientes, quanto dos profissionais, revelando as limitações dos sistemas de saúde diante de crises em grande escala, como a pandemia da COVID-19. Buscou-se, por meio do presente trabalho, apresentar as iniciativas de profissionais e empresas relacionadas à impressão 3D de compartilharem programas e recursos com o intuito de abastecer trabalhadores na linha de frente, que têm sido importantes aliados no combate aos efeitos provocados pelo novo coronavírus. Para isso, foi realizada uma pesquisa em quatro bases de dados eletrônicas, sendo elas: SCOPUS, BVS, EMBASE e MEDLINE via PUBMED. Após análise dos critérios de inclusão e exclusão, foi confeccionada uma tabela de resultados com as principais características dos produtos fabricados e disponibilizados, assim como a instituição envolvida. Concluiu-se, portanto, que as aplicações da impressão 3D nessas iniciativas tiveram grandes contribuições para a saúde pública e a indústria aditiva durante o período da pandemia da COVID-19.

ABSTRACT The fast spread of COVID-19 around the world has generated significant impacts on the way societies lives, one of which is the scarcity of personal protective equipment (PPE) for workers in the front lines of combat against the virus. Sanitary insecurity has led populations and health professionals to a rampant search for protective equipment and other devices necessary for life maintenance, both for patients and professionals, revealing health system limitations during large-scale crisis, as the COVID-19 pandemic. On this paper, we aimed to present the initiatives of professionals and companies related to 3D printing to share programs and resources whose purpose is supplying workers in the front line, who have been important allies in combating the effects caused by coronavirus. A search was performed in four electronic databases: SCOPUS, BVS, EMBASE and MEDLINE via PUBMED. After analyzing the inclusion and exclusion criteria, a table of results was prepared with the main characteristics of the products manufactured and made available, as well as the institutions involved. It was concluded, therefore, that the applications of 3D printing in the initiatives had great contributions to public health and the additive industry during the pandemic period of COVID-19.

Resumo Avaliou-se, entre idosos brasileiros, se o uso dos serviços odontológicos provenientes do Sistema Único de Saúde (SUS) é equânime. Utilizaram-se dados individuais de exames conduzidos por profissionais calibrados do levantamento nacional de saúde bucal (2010) e dados contextuais originários do DATASUS e do IBGE. A variável dependente foi o tipo de serviço utilizado: SUS e Outros. Foram conduzidas análises multiníveis através de regressão logística (α = 5%) (OR/IC 95%), através do SPSS 24.0. Participaram 6.303 idosos, a prevalência do uso no SUS foi de 30%, os fatores contextuais associados foram o acesso a banheiro e a água encanada (1,54/1,19-2,00) e o índice de cuidados odontológicos (1,41/1,10-1,81); já os individuais: idade (0,77/0,66-0,90), anos de estudo (1,83/1,53-2,20), renda familiar (2,57/2,20-3,01), motivo da última consulta (0,75/0,60-0,93), no de dentes cariados (1,26/1,08-1,48), no de dentes obturados (0,63/0,54-0,74), uso de próteses (2,23/1,91-2,59), dor de dente (1,36/1.11-1,67), autopercepção da necessidade de tratamento odontológico (1,20/1,12-1,51) e da necessidade de próteses (1,38/1,20-159). O uso no SUS tem sido equânime, porém é preciso organizar o processo de trabalho, viabilizando tal uso de forma regular e preventiva buscando a universalidade e a integralidade.

Abstract This study evaluated whether the use of dental services of the Unified Health System (SUS) by elderly Brazilians is equitable. Individual data collected by qualified professionals during the national oral health survey (2010) and contextual data from DATASUS and IBGE were used. The dependent variable was the type of service used: SUS and others. Multilevel analyses were conducted using logistic regression (α=5%) (OR/CI 95%) using SPSS 24.0. A total of 6,303 elderly people were included and the prevalence of dental service use in SUS was 30%. Associated contextual factors were: access to bathroom and running water (1.54/1.19-2.00) and the dental care index (1.41/1.10-1.81); the individual factors were: age (0.77/0.66-0.90), years of schooling (1.83/1.53-2.20), family income (2.57/2.20-3.01), reason for last dental visit (0.75/0.60-0.93), decayed teeth total (1.26/1.08-1.48), plugged teeth total (0.63/0.54-0.74), dental prosthesis use (2.23/1.91-2.59), dental pain (1.36/1.11-1.67), self-perception of the need for dental treatment (1.20/1.12-1.51) and need for dental prosthesis (1.38/1.20-1.59). Dental service from SUS has been equitable, however it is necessary to organize the working process, enabling its use in a regular and preventive manner, thereby seeking universality and comprehensiveness.

In December 2019, the first case of severe acute respiratory syndrome (COVID-19), caused by the new coronavirus (SARS-CoV-2) was reported and the disease was declared as a worldwide pandemic in March 2020. As there is no vaccine currently available, the daily hygiene cleaning habits and social distance are the ways to fight COVID-19 indicated by the WHO. Hand and surface hygiene has become essential and the use of 60-90% ethanol is recommended for its effective biocide action. Phenol-based products are effective sanitizers, but they can cause irritation and are not recommended for surfaces that come into contact with food. There are quaternary ammonium salts that present low toxic and are good sanitizers, as they can cause the denaturation of proteins and disruption of viral lipid bilayer. Sodium hypochlorite is one of the most used sanitizers due to its low cost and effective oxidative capacity. In the same direction, peroxides are also used as a biocide, as they cause oxidation of lipids and denaturation of viral proteins. The goal of this paper is to describe the action modes and chemical concepts of the sanitizers recommended by the ANVISA-Brazil to be used against the new coronavirus.

OBJECTIVES: This study aimed to analyze the efficiency of physiotherapy techniques in sputum induction and in the evaluation of pulmonary inflammation in asthmatic children and adolescents. Although hypertonic saline (HS) is widely used for sputum induction (SI), specific techniques and maneuvers of physiotherapy (P) may facilitate the collection of mucus in some asthmatic children and adolescents. METHODS: A randomized crossover study was performed in patients with well-controlled asthma, and 90 sputum samples were collected. Children and adolescents were assessed using spirometry and randomized at entry into one of three sputum induction techniques: (i) 3% hypertonic saline – HS technique; (ii) physiotherapy (oscillatory positive expiratory pressure, forced expiration, and acceleration of expiratory flow) – P technique; and (iii) hypertonic saline + physiotherapy – HSP technique. ClinicalTrials.gov: NCT03136042. RESULTS: The total cells (mL) and the percentage (%) of differential inflammatory cells were similar in all techniques. The sputum weight (g) in the HSP technique was significantly higher than that in the HS technique. In all techniques, the percentage of viable cells was >50%, and there was no difference between the HS and P techniques. Moreover, sputum induction did not cause any alterations in the pulmonary function of patients. CONCLUSION: The physiotherapy sputum collection technique was effective in obtaining viable cells from mucus samples and yielded the same amount of sputum as the gold standard technique (hypertonic saline). In addition, the physiotherapy maneuvers were both safe and useful for sputum induction in asthmatic children and adolescents with well-controlled asthma.

Resumo Avaliou-se, entre idosos brasileiros, se o uso dos serviços odontológicos provenientes do Sistema Único de Saúde (SUS) é equânime. Utilizaram-se dados individuais de exames conduzidos por profissionais calibrados do levantamento nacional de saúde bucal (2010) e dados contextuais originários do DATASUS e do IBGE. A variável dependente foi o tipo de serviço utilizado: SUS e Outros. Foram conduzidas análises multiníveis através de regressão logística (α = 5%) (OR/IC 95%), através do SPSS 24.0. Participaram 6.303 idosos, a prevalência do uso no SUS foi de 30%, os fatores contextuais associados foram o acesso a banheiro e a água encanada (1,54/1,19-2,00) e o índice de cuidados odontológicos (1,41/1,10-1,81); já os individuais: idade (0,77/0,66-0,90), anos de estudo (1,83/1,53-2,20), renda familiar (2,57/2,20-3,01), motivo da última consulta (0,75/0,60-0,93), no de dentes cariados (1,26/1,08-1,48), no de dentes obturados (0,63/0,54-0,74), uso de próteses (2,23/1,91-2,59), dor de dente (1,36/1.11-1,67), autopercepção da necessidade de tratamento odontológico (1,20/1,12-1,51) e da necessidade de próteses (1,38/1,20-159). O uso no SUS tem sido equânime, porém é preciso organizar o processo de trabalho, viabilizando tal uso de forma regular e preventiva buscando a universalidade e a integralidade.

Abstract This study evaluated whether the use of dental services of the Unified Health System (SUS) by elderly Brazilians is equitable. Individual data collected by qualified professionals during the national oral health survey (2010) and contextual data from DATASUS and IBGE were used. The dependent variable was the type of service used: SUS and others. Multilevel analyses were conducted using logistic regression (α=5%) (OR/CI 95%) using SPSS 24.0. A total of 6,303 elderly people were included and the prevalence of dental service use in SUS was 30%. Associated contextual factors were: access to bathroom and running water (1.54/1.19-2.00) and the dental care index (1.41/1.10-1.81); the individual factors were: age (0.77/0.66-0.90), years of schooling (1.83/1.53-2.20), family income (2.57/2.20-3.01), reason for last dental visit (0.75/0.60-0.93), decayed teeth total (1.26/1.08-1.48), plugged teeth total (0.63/0.54-0.74), dental prosthesis use (2.23/1.91-2.59), dental pain (1.36/1.11-1.67), self-perception of the need for dental treatment (1.20/1.12-1.51) and need for dental prosthesis (1.38/1.20-1.59). Dental service from SUS has been equitable, however it is necessary to organize the working process, enabling its use in a regular and preventive manner, thereby seeking universality and comprehensiveness.

RESUMO Objetivo: Avaliar se o azul de metileno (AM) pode minimizar os efeitos da lesão de isquemia-reperfusão sobre o pulmão não isquêmico em um modelo de transplante pulmonar em roedores. Métodos: Quarenta ratas Sprague-Dawley foram divididas em 20 doadoras e 20 receptoras. As 20 ratas receptoras foram divididas em dois grupos (n = 10) de acordo com o tratamento (solução salina a 0,9% vs. AM a 1%). Todos os animais foram submetidos a transplante pulmonar unilateral. As receptoras receberam 2 ml de solução salina ou de AM por via intraperitoneal antes do transplante. Após 2 h de reperfusão, os animais foram sacrificados, e foram realizadas análises histopatológicas e imuno-histoquímicas no pulmão não isquêmico. Resultados: Houve diminuição significativa da inflamação - a contagem de neutrófilos e a expressão de intercellular adhesion molecule-1 (ICAM-1, molécula de adesão intercelular-1) foram maiores no grupo salina em comparação com o grupo AM - e da apoptose - a expressão de caspase-3 foi maior no grupo salina, e a expressão de Bcl-2 foi maior no grupo AM. Conclusões: O AM é uma droga eficaz para a proteção de pulmões não isquêmicos contra inflamação e apoptose após transplante pulmonar unilateral em ratos.

ABSTRACT Objective: To evaluate whether methylene blue (MB) could minimize the effects of ischemia-reperfusion injury in the nonischemic lung on a lung transplantation rodent model. Methods: Forty female Sprague-Dawley rats were divided into 20 donors and 20 recipients. The 20 recipient rats were divided into two groups (n = 10) according to the treatment (0.9% saline vs. 1% MB solutions). All animals underwent unilateral lung transplantation. Recipients received 2 mL of saline or MB intraperitoneally prior to transplantation. After 2 h of reperfusion, the animals were euthanized and histopathological and immunohistochemical analyses were performed in the nonischemic lung. Results: There was a significant decrease in inflammation-neutrophil count and intercellular adhesion molecule-1 (ICAM-1) expression in lung parenchyma were higher in the saline group in comparison with the MB group-and in apoptosis-caspase-3 expression was higher in the saline group and Bcl-2 expression was higher in MB group. Conclusions: MB is an effective drug for the protection of nonischemic lungs against inflammation and apoptosis following unilateral lung transplantation in rats.

Resumo Leptophlebiidae é a família com maior diversidade de espécies e com maior distribuição no Hemisfério Sul. Existem 95 espécies de Leptophlebiidae registradas no Brasil, mas no estado da Bahia existem apenas 21 espécies. Uma lista e os novos registros de Leptophlebiidae (Ephemeroptera) são apresentados ao Estado da Bahia. Um total de 16 gêneros e 40 espécies de Leptophlebiidae foram identificadas neste trabalho. As espécies Fittkaulus cururuensis, Hylister obliquus, Miroculis (atroari) duckensis e Miroculis (Ommaethus) froehlichi são relatadas pela primeira vez no Estado da Bahia, aumentando o número de espécies de Leptophlebiidae de 21 para 25. Agora, a Bahia é um dos estados com o maior número de registros de espécies de Leptophlebiidae, atrás apenas do estado do Espírito Santo com 26 espécies desta família.

Abstract Leptophlebiidae is the family with the most diversity of species and wide distribution in the Southern Hemisphere. There are 95 species of Leptophlebiidae recorded in Brazil, but for the state of Bahia there are only 21 species. A check list and new records of Leptophlebiidae (Ephemeroptera) are presented to the State of Bahia. A total of 16 genera and 40 species of Leptophlebiidae were identified on this study. The species Fittkaulus cururuensis, Hylister obliquus, Miroculis (atroari) duckensis and Miroculis (Ommaethus) froehlichi are reported for the first time to the State of Bahia, increasing the number of Leptophlebiidae species from 21 to 25. Now, the Bahia is one of the states with the largest number of Leptophlebiidae species records, behind only of the state of Espírito Santo which has 26 species of this family.

Resumo É recente a preocupação em delimitar a pesquisa com crianças e, mais importante, o quanto é indispensável pensá-la sob uma perspectiva ética. Com vistas à ampliação dessa discussão, objetivou-se oferecer uma forma de abordagem ética que se acredita ser essencial para o desenvolvimento de pesquisas com crianças a partir do pensamento do filósofo Emmanuel Lévinas. Com base na alteridade de Lévinas, concluiu-se que para realização de pesquisa com crianças torna-se necessário se abrir para o mundo infantil, totalmente diverso e autônomo. Perceber essa necessidade é perceber a imprescindibilidade da alteridade como pressuposto ético das relações humanas, no caso, entre o pesquisador e a criança. Somente assim, a autonomia, o respeito e a participação ativa da criança estarão assegurados como um direito que possui não apenas por ser participante de pesquisa, mas também como ser humano, com as particularidades que devem ser consideradas efetivamente.

Abstract Concern over delimiting research involving children, and more importantly the necessity of considering such research from an ethical perspective, is a recent development. With the aim of broadening this discussion, a form of ethical approach believed to be essential for the development of research with children was sought, based on the thinking of the philosopher Emmanuel Lévinas. Based on the otherness of Lévinas, it was concluded that for research with children, it is necessary to open oneself up to their world, and treat it as entirely diverse and autonomous. Understanding this necessity is to perceive the indispensability of otherness as an ethical presupposition of human relationships, in this case, between the researcher and the child. Only in this way will the autonomy, respect and active participation of the child be assured as a right not only in the role of a research participant but also a human being with individual characteristics that must be effectively considered.

Resumen Es reciente la preocupación por delimitar la investigación con niños y, lo que es más importante, lo indispensable que resulta pensarla desde una perspectiva ética. Tendiendo a una ampliación de esta discusión, se tuvo como objetivo ofrecer una forma de abordaje ético que se considera es esencial para el desarrollo de investigaciones con niños a partir del pensamiento del filósofo Emmanuel Lévinas. En base a la alteridad de Lévinas, se concluyó que para la realización de la investigación con niños se hace necesario abrirse al mundo infantil, totalmente diverso y autónomo. Percibir esa necesidad es percibir la imprescindibilidad de la alteridad como un presupuesto ético de las relaciones humanas, en este caso, entre el investigador y el niño. Sólo así, la autonomía, el respeto y la participación activa del niño estarán asegurados como un derecho que posee no sólo por ser un participante de la investigación, sino también por ser un ser humano con las particularidades que deben ser consideradas efectivamente.

ABSTRACT Objective: to analyze the management of mental health needs in primary care as perceived by Family Health Strategy professionals. Method: this was a qualitative descriptive exploratory study developed within the coverage area of five family health teams. The data were collected using observation, group interviews, individual semi-structured interviews, and focus groups. Content analysis was conducted using text analysis software and interpretation was based on the corresponding analytical structures. Results: numerous and challenging mental health demands occur in this setting, for which the teams identified care resources; however, they also indicated difficulties, especially related to the operationalization and integration of such resources. Conclusion: there is a need for a care network sensitive to mental health demands that are better coordinated and more effectively managed.

RESUMEN Objetivo: analizar el manejo de necesidades de salud mental en la atención primaria de salud, según la visión de los profesionales de la Estrategia Salud de la Familia. Método: estudio cualitativo, descriptivo, exploratorio, desarrollado en el campo de acción de cinco equipos de salud familiar. Participaron cinco enfermeras, cinco coordinadores y diecisiete agentes comunitarios de salud. Datos recolectados mediante observación, entrevistas grupales, entrevistas individuales semiestructuradas y grupos focuses. Se aplicó análisis de contenido, con ayuda de software de análisis textual. La interpretación se basó en las correspondientes estructuras analíticas. Resultados: numerosas y desafiantes demandas de salud mental fueron recogidas en este setting, para las cuales los equipos identificaron recursos de atención; aunque detectaron dificultades, particularmente relacionadas a la puesta en marcha e integración de tales recursos. Conclusión: se enfatiza la necesidad de una red de atención sensible a tales demandas, aunque articulada y gerenciada de manera eficaz.

RESUMO Objetivo: analisar o manejo das necessidades de saúde mental na atenção primária à saúde de acordo com a percepção dos profissionais da Estratégia Saúde da Família. Método: estudo qualitativo, descritivo exploratório, desenvolvido no território de abrangência de cinco equipes de saúde da família. Os participantes foram cinco enfermeiras, cinco coordenadores e 17 agentes comunitários de saúde. Os dados foram coletados utilizando observação, entrevistas grupais, entrevistas individuais semiestruturadas e grupos focais. Fez-se a análise de conteúdo com o auxílio de um Software de análise textual, e a interpretação baseou-se nas estruturas analíticas correspondentes. Resultados: inúmeras e desafiadoras demandas de saúde mental têm sido acolhidas nesse setting, para as quais as equipes identificaram recursos de atendimento; no entanto, apontaram dificuldades, sobretudo relacionadas à operacionalização e integração destes recursos. Conclusão: destaca-se a necessidade de uma rede de cuidados sensível a tais demandas, mais articulada e gerida de modo eficaz.

RESUMO Objetivo: analisar o manejo das necessidades de saúde mental na atenção primária à saúde de acordo com a percepção dos profissionais da Estratégia Saúde da Família. Método: estudo qualitativo, descritivo exploratório, desenvolvido no território de abrangência de cinco equipes de saúde da família. Os participantes foram cinco enfermeiras, cinco coordenadores e 17 agentes comunitários de saúde. Os dados foram coletados utilizando observação, entrevistas grupais, entrevistas individuais semiestruturadas e grupos focais. Fez-se a análise de conteúdo com o auxílio de um Software de análise textual, e a interpretação baseou-se nas estruturas analíticas correspondentes. Resultados: inúmeras e desafiadoras demandas de saúde mental têm sido acolhidas nesse setting, para as quais as equipes identificaram recursos de atendimento; no entanto, apontaram dificuldades, sobretudo relacionadas à operacionalização e integração destes recursos. Conclusão: destaca-se a necessidade de uma rede de cuidados sensível a tais demandas, mais articulada e gerida de modo eficaz.

ABSTRACT Objective: to analyze the management of mental health needs in primary care as perceived by Family Health Strategy professionals. Method: this was a qualitative descriptive exploratory study developed within the coverage area of five family health teams. The data were collected using observation, group interviews, individual semi-structured interviews, and focus groups. Content analysis was conducted using text analysis software and interpretation was based on the corresponding analytical structures. Results: numerous and challenging mental health demands occur in this setting, for which the teams identified care resources; however, they also indicated difficulties, especially related to the operationalization and integration of such resources. Conclusion: there is a need for a care network sensitive to mental health demands that are better coordinated and more effectively managed.

RESUMEN Objetivo: analizar el manejo de necesidades de salud mental en la atención primaria de salud, según la visión de los profesionales de la Estrategia Salud de la Familia. Método: estudio cualitativo, descriptivo, exploratorio, desarrollado en el campo de acción de cinco equipos de salud familiar. Participaron cinco enfermeras, cinco coordinadores y diecisiete agentes comunitarios de salud. Datos recolectados mediante observación, entrevistas grupales, entrevistas individuales semiestructuradas y grupos focuses. Se aplicó análisis de contenido, con ayuda de software de análisis textual. La interpretación se basó en las correspondientes estructuras analíticas. Resultados: numerosas y desafiantes demandas de salud mental fueron recogidas en este setting, para las cuales los equipos identificaron recursos de atención; aunque detectaron dificultades, particularmente relacionadas a la puesta en marcha e integración de tales recursos. Conclusión: se enfatiza la necesidad de una red de atención sensible a tales demandas, aunque articulada y gerenciada de manera eficaz.

OBJECTIVE: To evaluate the respiratory systems of male and female rats maintained in individually ventilated cages (IVCs) from birth until adulthood. METHODS: Female Wistar rats were housed in individually ventilated cages or conventional cages (CCs) and mated with male Wistar rats. After birth and weaning, the male offspring were separated from the females and kept in cages of the same type until 12 weeks of age. RESULTS: The level of food consumption was lower in male offspring (IVC=171.7±9; CC=193.1±20) than in female offspring (IVC=100.6±7; CC=123.4±0.4), whereas the water intake was higher in female offspring (IVC=149.8±11; CC=99.2±0) than in male offspring (IVC=302.5±25; CC=249.7±22) at 11 weeks of age when housed in IVCs. The cage temperature was higher in individually ventilated cages than in conventional cages for both male (IVCs=25.9±0.5; CCs=22.95±0.3) and female (IVCs=26.2±0.3; CCs=23.1±0.3) offspring. The respiratory resistance (IVC=68.8±2.8; CC=50.6±3.0) and elastance (IVC=42.0±3.9; CC=32.4±2.0) at 300 µm/kg were higher in the female offspring housed in ventilated cages. The ciliary beat values were lower in both the male (IVCs=13.4±0.2; CC=15±0.4) and female (IVC=13.5±0.4; CC=15.9±0.6) offspring housed in individually ventilated cages than in those housed in conventional cages. The total cell (IVC=117.5±9.7; CC=285.0±22.8), neutrophil (IVC=13.1±4.8; CC=75.6±4.1) and macrophage (IVC=95.2±11.8; CC=170.0±18.8) counts in the bronchoalveolar lavage fluid were lower in the female offspring housed in individually ventilated cages than in those housed in conventional cages. CONCLUSIONS: The environmental conditions that exist in individually ventilated cages should be considered when interpreting the results of studies involving laboratory animals. In this study, we observed gender dimorphism in both the water consumption and respiratory mechanics of rats kept in ventilated cages.

RESUMO Objetivo: elaborar, avaliar e validar um checklist de segurança cirúrgica para os períodos pré e pós-operatório de unidades de internação cirúrgica. Método: pesquisa metodológica, realizada em hospital de ensino público de grande porte do Sul do Brasil, com aplicação dos fundamentos do Programa Cirurgias Seguras Salvam Vidas da Organização Mundial da Saúde. O checklist foi aplicado a 16 enfermeiros de oito unidades cirúrgicas, e submetido à validação por meio da técnica Delphi on-line com oito especialistas. Resultados: o instrumento foi validado, obtendo-se ranking médio ≥1, grau de concordância ≥75% e Alfa de Cronbach >0,90. A versão final contemplou 97 indicadores de segurança organizados em seis categorias: identificação, pré-operatório, pós-operatório imediato, pós-operatório mediato, outras complicações cirúrgicas, e alta hospitalar. Conclusão: o Checklist de Segurança Cirúrgica Pré e Pós-Operatório é mais uma estratégia na promoção da segurança do paciente, pois possibilita monitorar sinais e sintomas preditivos de complicações cirúrgicas e detecção precoce de eventos adversos.

ABSTRACT Objective: to develop, evaluate and validate a surgical safety checklist for patients in the pre and postoperative periods in surgical hospitalization units. Method: methodological research carried out in a large public teaching hospital in the South of Brazil, with application of the principles of the Safe Surgery Saves Lives Programme of the World Health Organization. The checklist was applied to 16 nurses of 8 surgical units and submitted for validation by a group of eight experts using the Delphi method online. Results: the instrument was validated and it was achieved a mean score ≥1, level of agreement ≥75% and Cronbach’s alpha >0.90. The final version included 97 safety indicators organized into six categories: identification, preoperative, immediate postoperative, immediate postoperative, other surgical complications, and hospital discharge. Conclusion: the Surgical Safety Checklist in the Pre and Postoperative periods is another strategy to promote patient safety, as it allows the monitoring of predictive signs and symptoms of surgical complications and the early detection of adverse events.

RESUMEN Objetivo: desarrollar, evaluar y validar un checklist de seguridad quirúrgica para los períodos pre y postoperatorio de unidades de hospitalización quirúrgica.. Método: investigación metodológica llevada a cabo en un amplio hospital público de enseñanza del Sur de Brasil, con aplicación de los principios del Programa de Cirugía Segura Salva Vidas de la Organización Mundial de la Salud. El checklist se aplicó a 16 enfermeros de 8 unidades quirúrgicas y fue sometida a validación por ocho expertos utilizando el método Delphi en línea. Resultados: el instrumento fue validado y se logró una puntuación media ≥1, grado de acuerdo ≥75% y alfa de Cronbach >0.90. La versión final incluyó 97 indicadores de seguridad organizados en seis categorías: identificación, preoperatorio, postoperatorio inmediato, postoperatorio inmediato, otras complicaciones quirúrgicas y alta hospitalaria. Conclusión: el Checklist de Seguridad Quirúrgica en el Período Pre y Postoperatorio es otra estrategia más para promover la seguridad del paciente, ya que permite monitorear los signos y síntomas predictivos de las complicaciones quirúrgicas y la detección temprana de eventos adversos.