ABSTRACT Stress caused by defoliation can compromise the physiological quality of soybean seeds. However, there is evidence that the application of nitrogen (N) can be an alternative to reduce the qualitative losses in seeds caused by defoliating insects. The objective of this study was to evaluate the effectiveness of topdressing N fertilization in improving the physiological quality of soybean seeds from plants subjected to artificial defoliation. The experimental design was a randomized block arranged in a 2×4 factorial scheme with four replicates. The treatments consisted of two levels of defoliation (33 and 66%) and four doses of N (0, 50, 100, and 150 kg ha-1), using urea (45% N), applied two days after defoliation. Artificial defoliation was performed during the R3 stage by removing one (33%) and two leaflets (66%) from each trifoliate leaf under field conditions. At stage R5.1, leaf area was quantified, and after harvesting, germination, emergence, length, total dry mass, electrical conductivity, total protein content, mass of 1000 seeds, and tetrazolium (vigor and viability) tests were carried out. It was concluded that increasing the defoliation from 33 to 66% impaired the physiological quality of soybean seeds. The use of nitrogen fertilization for coverage after defoliation at the beginning of the reproductive phase cannot minimize qualitative losses in seeds.

RESUMO: A germinação e o crescimento de plantas de melão (Cucumis melo L.) podem ser severamente afetadas pelo excesso de sais no solo ou na água de irrigação. No entanto, os efeitos negativos do estresse salino podem ser amenizados com o uso de métodos adequados de condicionamento das sementes. Assim, este estudo objetivou investigar os efeitos do osmocondicionamento como indutor da tolerância ao estresse salino em sementes de melão expostas à níveis de salinidade. As sementes foram imersas por 22 h à 25 °C em água destilada (hidrocondicionamento) ou em solução de 0,5% de KNO3 (osmocondicionamento). Após secagem, as sementes foram distribuídas em caixas plásticas com papel mata-borrão contendo as diferentes soluções de NaCl preparadas com potencial osmótico de 0,0 MPa (controle), -0,3 MPa (estresse suave) e -0,6 MPa (estresse severo). Um tratamento com sementes secas não condicionadas foi utilizado como controle. As caixas plásticas foram mantidas em germinador de sementes, a 25 °C por 14 dias. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 3 × 3, com quatro repetições de 25 sementes. Os resultados mostraram que o condicionamento de sementes com água e KNO3 pode ser aplicado com sucesso em sementes de melão para amenizar os efeitos adversos do estresse salino durante o estágio inicial de crescimento das plantas. No entanto, sob estresse salino severo, o hidrocondicionamento deve ser utilizado por resultar em maior percentagem de germinação e maior taxa de crescimento inicial das plântulas quando comparado ao osmocondicionamento. O uso de sementes de melão sem condicionamento prévio não deve ser adotado em áreas de cultivo afetadas pela salinidade, pois resultam em baixa taxa de germinação e reduzido crescimento inicial das plantas.

ABSTRACT: The germination and growth of melon (Cucumis melo L.) plants can be severely affected by excess salts in the soil or irrigation water; however, negative effects of salt stress can be attenuated using appropriate methods of seed priming. Thus, effects of osmopriming as inducer of salt stress tolerance in melon seeds exposed to salinity levels were investigated in this study. Seeds were soaked for 22 h at 25 °C in the dark in distilled water (hydropriming) or 0.5% KNO3 solution (osmopriming), and after drying, were distributed in plastic boxes with blotter paper containing different NaCl solutions prepared with osmotic pressure of 0.0 MPa (control), -0.3 MPa (mild stress), and -0.6 MPa (severe stress). Unprimed dry seeds were taken as control. The plastic boxes were kept into a seed germinator, at 25 °C for 14 days. A completely randomized design in a 3 × 3 factorial schemes with four replicates of 25 seeds was used. Results showed that the seed priming with water and KNO3 may be successfully applied on melon seeds to alleviate the adverse effects of saline stress in initial stages of plant growth. However, under severe salt stress conditions, hydropriming should be used because it results in higher germination and initial growth rate of the seedlings when compared to the osmopriming. Use of unprimed seeds should not be adopted in cultivation areas affected by salinity because they result in low germination rate and reduced initial plant growth.