Abstract Qilian Huaji Decoction (QLHJD) is a mixture of 13 herbs. The aim of this study is to observe the efficacy of QLHJD against hepatocellular carcinoma (HCC). The H22 transplanted mouse models were established to explore the effect of QLHJD on HCC in vivo. QLHJD-containing serum (QCS) was prepared using traditional Chinese medicine serum pharmacology methods. After QCS treatment, cell proliferation, cell cycle and apoptosis in HepG2 cells were assessed by MTT and flow cytometry, respectively. miRNA expression profiles were determined using qPCR arrays. miR-122 was examined by qPCR in HCC tissue samples and cells. AKT3 protein expression was detected by Western blot. We found that QLHJD significantly inhibited the growth of H22 tumor and improve immune function in vivo. In vitro, QLHJD inhibited proliferation by inducing cell cycle arrest at the S phase, and induced apoptosis in HepG2 cells. The miRNA expression profiles were altered and the reduced miR-122 levels in HepG2 cell lines were up-regulated compared with control group, accompanied by a decrease in WNT1 expression. In conclusion, QLHJD could inhibit the growth of HCC by restoring the aberrant expression of miR-122, which may be one of the mechanisms whereby QLHJD exerts its effect on HCC.

Abstract The embrittlement and softening behaviors in heat affected zone of X90 pipeline steel were studied, and the influence of weld thermal cycle on microstructure and mechanical properties were discussed by combining the effect of peak temperature on the phase transition point (Ar3). Results show that there are embrittlement and softening zones existed in heat affected zone of X90 pipeline steel. The peak temperature greatly affected the austenitizing and its grain size, thereby affected phase transition during cooling process. The embrittlement in critical heat affected zone at 750 °C was caused by brittle bainite and M /A islands formed during cooling, because little austenite was formed during heating. While, the embrittlement in coarse grain heat affected zone was caused by austenite grain coarsen, which transformed to the coarse bainite after cooling. The softening zone attributed to the polygonal ferrite and granular ferrite after cooling, which came from fine and ununiformed austenite formed during reheating process. Ar3 decreased with the increase of Cu, Cr or Mo content in steel, and the BF content in CGHAZ and GB and AF content in FGHAZ increased.