Abstract Background: exercise can increase the species and quantity of beneficial gut microbiota, enrich the diversity of microflora, and promote the development of symbiotic bacteria, especially in the stage of ontogeny. However, there is little evidence of the short-term voluntary exercise effect on the gut microbiota in developing mice. Material and method: therefore, we used short-term voluntary wheel running model to study the gut microbiota of developing mice (1 month old), and detected the fecal samples by 16S rRNA gene sequencing. Results: the results showed that after 4 weeks of voluntary wheel running, the body weight of the running group was significantly lower than that of the control group. Conclusion: there was a significant separation between the running group and the control group in beta diversity measures. At the family level, the clostridiales flora of the running group was higher than that of the control group. Compared with the control group, the abundance of parabacteroides flora and anaerovorax flora increased significantly, and the abundance of anaerotruncus flora and odoribacter flora decreased significantly in the running group. These results showed that gut microbiota be affected after short-term voluntary wheel running in developing mice.

Resumen Introducción: el ejercicio puede aumentar las especies y la cantidad de microbiota intestinal beneficiosa, enriquecer la diversidad de la microflora y promover el desarrollo de bacterias simbióticas, especialmente en la etapa de ontogenia. Sin embargo, hay poca evidencia del efecto del ejercicio voluntario a corto plazo sobre la microbiota intestinal en ratones en desarrollo. Material y método: por lo tanto, utilizamos un modelo de carrera de ruedas voluntario a corto plazo para estudiar la microbiota intestinal de ratones en desarrollo (1 mes de edad) y detectamos las muestras fecales mediante la secuenciación del gen 16S rRNA. Resultados: los resultados mostraron que después de 4 semanas de carrera voluntaria con ruedas, el peso corporal del grupo de carrera fue significativamente más bajo que el del grupo de control. Conclusión: hubo una diferencia significativa entre el grupo de corredores y el grupo de control en las medidas de diversidad beta. A nivel familiar, la flora de clostridiales del grupo de corredores fue mayor que la del grupo de control. En comparación con el grupo de control, la abundancia de flora parabacteroides y flora anaerovorax aumentó significativamente, y la abundancia de flora anaerotruncus y flora odoribacter disminuyó significativamente en el grupo de carrera. Estos resultados mostraron que la microbiota intestinal se ve afectada después de la carrera voluntaria a corto plazo en ratones en desarrollo.

Given their relationship with metabolic syndrome and systematic inflammatory diseases, the pathogenesis of hypertension, hyperglycemia, and hyperlipidemia is closely related. To explore the common genes among these three conditions, spontaneous hypertensive rats (SHR), spontaneous diabetic Goto-Kakizaki rats (GK) and hyperlipidemia rats (HMR) were reared for experiments. Gene array was used to identify the genes of SHR, GK and HMR compared with normal Wistar rats using TBtools software. First, real-time PCR was applied to verify these genes, and Cytoscape software was used to construct networks based on the National Center for Biotechnology Information (NCBI) database. Second, Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis was performed to classify the genes. Visualization and Integrated Discovery (DAVID) database and Gene Ontology database were used to explore the biological function. Finally, Onto-tools Pathway Express was used to analyze the pathways of shared genes. Importantly, upregulated common genes, such as Bad, Orm1, Arntl and Zbtb7a, were used to construct a network of 150 genes, while downregulated genes, such as Mif and Gpx1, formed a network of 29 genes. Interestingly, the networks were involved in various pathways, such as insulin signal pathway, endometrial cancer pathway, circadian rhythm pathway, and pancreatic cancer pathway. We discovered common genes of SHR, GK and HMR compared with normal Wistar rats, and the association of these genes together with biological function were preliminarily revealed.

Abstract The Asian gypsy moth (Lymantria dispar) is a serious pest of forest and shade trees in many Asian and some European countries. However, there have been few studies of L. dispar genetic information and comprehensive genetic analyses of this species are needed in order to understand its genetic and metabolic sensitivities, such as the molting mechanism during larval development. In this study, high-throughput sequencing technology was used to sequence the transcriptome of the Asian subspecies of the gyspy moth, after which a comprehensive analysis of chitin metabolism was undertaken. We generated 37,750,380 high-quality reads and assembled them into contigs. A total of 37,098 unigenes were identified, of which 15,901 were annotated in the NCBI non-redundant protein database and 9,613 were annotated in the Swiss-Prot database. We mapped 4,329 unigenes onto 317 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database. Chitin metabolism unigenes were found in the transcriptome and the data indicated that a variety of enzymes was involved in chitin catabolic and biosynthetic pathways.

A elevação da quantidade de mudas de alta qualidade associada ao descarte das de baixa qualidade pode aprimorar os programas de seleção e melhoramento genético da cana-de-açúcar (Saccharum spp.). Este trabalho avaliou a capacidade do método Dynamic Technique for Order Preference by Similarity to Ideal Solution (DTOPSIS) de identificar famílias da cana-de-açúcar com elevada qualidade. As características avaliadas foram: Brix, aptidão para moagem, diâmetro do caule, altura e porcentagem de tecido parenquimatoso. Os dados foram coletados em dois experimentos com 17 famílias e duas cultivares incluindo cana soca e brota. O DTOPSIS estima o índice Ci que expressa a proximidade de uma solução particular à ideal. O Ci foi utilizado para avaliar a distância de cada família à família ideal. As demais mudas foram utilizadas em um programa tradicional de seleção. Os valores de Ci de cada família foram comparados às taxas de seleção de famílias obtidas pelo programa tradicional de seleção por meio da estimação das taxas de seleção nos estágios 1 a 4. Os resultados indicaram que os valores de Ci calculados a partir dos experimentos foram significativamente correlacionados (p<0,01) às taxas de seleção do estágio 2 (r=0,8059), estágio 3 (r=0,7967) e estágio 4 (r=0,8202). Os resultados também indicaram que clones com promissor uso apresentam elevados valores de Ci. Conclui-se, portanto, que o método DTOPSIS é apropriado para selecionar e descartar mudas de alta e baixa qualidade, respectivamente, podendo elevar a eficiência dos programas de seleção.

Enlarging the quantity of seedlings of elite families and discarding inferior sugarcane (Saccharum spp.) families could improve sugarcane breeding and selection efficiency. The feasibility of using the method Dynamic Technique for Order Preference by Similarity to Ideal Solution (DTOPSIS) method was explored to identify superior sugarcane families. Data on 5 traits: Brix, millable stalks per stool (MS), stalk diameter (SD), plant height (PH), and percent pith were collected from two family trials having 17 families and two check cultivars at two sites including plant-cane and first-ratoon crops. The rest of the seedlings were planted into field for routine selection in the regular program. The DTOPSIS method calculates a comprehensive index (Ci) which expresses the closeness of a solution to the ideal solution and was used in this study to test the distance of each family to the ideal family. The Ci of the families was compared to the family selection rate in the regular program by determining the selection rate at Stage 1 to Stage 4 for each family in the regular program. The result indicated that the Ci values calculated from family trials were significantly (p<0.01) correlated to the selection rate at Stage 2 (r=0.8059), Stage3 (r=0.7967), and Stage 4 (r=0.8202), and indicating that promising clones were selected from families with higher Ci values in the family trial. Thus, it could be feasible to use DTOPSIS to determine elite sugarcane families and to eliminate inferior families and thereby, increasing the variety selection efficiency.