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The reliability and validity of Child-to-parent Violence Questionnaire (CPV-Q) among Chinese adolescents
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Xie, Weishi

; Wang, Mengxuan Wang, Linya Yang, Li Luo, Min

Psicologia: Reflexão e Crítica

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2024, Volume 37 elocation 30

Abstract: En | Text: En | PDF: En

Abstract Objective To test the reliability and validity of the Chinese version of the Child-to-parent Violence Questionnaire (CPV-Q) in a group of Chinese adolescents. Methods A total of 1138 adolescents (15.24 ± 1.17 years old) were tested with the Chinese version of CPV-Q, Parent-Adolescent Conflict Scale, and Adolescent Aggressive Behavior Scale of which 201 adolescents were retested 1 month later. The Chinese version of CPV-Q contains psychological, physical, financial, and control/domain factors with 14 items. Results The four-factor model has good main fit indicators (father: χ2/df = 3.28, CFI = 0.96, RMSEA = 0.06; mother: χ2/df = 3.30, CFI = 0.96, RMSEA = 0.06); the scale has good criterion-related validity. The Cronbach’s a coefficients of the Chinese version of CPV-Q were 0.89 (father) and 0.88 (mother), and the Cronbach’s α coefficients of the four subscales were 0.81 ~ 0.84 (father) and 0.76 ~ 0.85 (mother). The test–retest reliability of the Chinese version of CPV-Q was 0.85 (father) and 0.83 (mother), and the test–retest reliability of the four subscales was 0.80 ~ 0.83 (father) and 0.75 ~ 0.84 (mother). Conclusion Therefore, the CPV-Q has good reliability and validity for Chinese adolescents and can be used as an effective tool to evaluate Chinese adolescents’ violence toward their parents.

https://doi.org/10.1186/s41155-024-00314-1

Intensive glucose control in critically ill adults: a protocol for a systematic review and individual patient data meta-analysis
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Adigbli, Derick

; Yang, Li

; Hammond, Naomi

; Annane, Djillali

; Arabi, Yaseen

; Bilotta, Federico

; Bohé, Julien

; Brunkhorst, Frank Martin

; Cavalcanti, Alexandre Biasi

; Cook, Deborah

; Engel, Christoph

; Green-LaRoche, Deborah

; He, Wei

; Henderson, William

; Hoedemaekers, Cornelia

; Iapichino, Gaetano

; Kalfon, Pierre

; Rosa, Gisela de La

; MacKenzie, Iain

; Mélot, Christian

; Mitchell, Imogen Oksanen, Tuomas Polli, Federico Preiser, Jean-Charles Soriano, Francisco Garcia Wang, Ling-Cong Yuan, Jiaxiang Delaney, Anthony Tanna, Gian Luca Di Finfer, Simon

Critical Care Science

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dec 2023, Volume 35 N. 4 Pages 345 - 354

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Não está claro qual é a meta ideal de concentração de glicose no sangue em pacientes em estado grave. Realizaremos uma revisão sistemática e uma metanálise com dados agregados e de pacientes individuais de estudos controlados e randomizados, comparando o controle intensivo da glicose com o controle liberal da glicose em adultos em estado grave. Fontes de dados: MEDLINE®, Embase, Cochrane Central Register of Clinical Trials e registros de ensaios clínicos (Organização Mundial da Saúde, clinical trials.gov). Os autores dos estudos qualificados serão convidados a fornecer dados individuais de pacientes. Os dados publicados em nível de ensaio qualificado que não apresentem alto risco de viés serão incluídos em uma metanálise de dados agregados se os dados individuais de pacientes não estiverem disponíveis. Métodos: Critérios de inclusão: ensaios clínicos controlados e randomizados que recrutaram pacientes adultos, com meta de glicemia ≤ 120mg/dL (≤ 6,6mmol/L) comparada a uma meta de concentração de glicemia mais alta com insulina intravenosa em ambos os grupos. Estudos excluídos: aqueles com meta de glicemia no limite superior no grupo de intervenção > 120mg/dL (> 6,6mmol/L), ou em que o controle intensivo de glicose foi realizado apenas no período intraoperatório, e aqueles em que a perda de seguimento excedeu 10% até a alta hospitalar. Desfecho primário: Mortalidade intra-hospitalar durante a admissão hospitalar. Desfechos secundários: Mortalidade e sobrevida em outros momentos, duração da ventilação mecânica invasiva, agentes vasoativos e terapia de substituição renal. Utilizaremos metanálise bayesiana de efeito randômico e modelos bayesianos hierárquicos para dados individuais de pacientes. Discussão: Essa revisão sistemática com dados agregados e de pacientes individuais abordará a questão clínica: Qual é a melhor meta de glicose no sangue de pacientes graves em geral? Protocolo versão 0.4 - 26/06/2023 Registro PROSPERO: CRD42021278869

ABSTRACT Objective: The optimal target for blood glucose concentration in critically ill patients is unclear. We will perform a systematic review and meta-analysis with aggregated and individual patient data from randomized controlled trials, comparing intensive glucose control with liberal glucose control in critically ill adults. Data sources: MEDLINE®, Embase, the Cochrane Central Register of Clinical Trials, and clinical trials registries (World Health Organization, clinical trials.gov). The authors of eligible trials will be invited to provide individual patient data. Published trial-level data from eligible trials that are not at high risk of bias will be included in an aggregated data meta-analysis if individual patient data are not available. Methods: Inclusion criteria: randomized controlled trials that recruited adult patients, targeting a blood glucose of ≤ 120mg/dL (≤ 6.6mmol/L) compared to a higher blood glucose concentration target using intravenous insulin in both groups. Excluded studies: those with an upper limit blood glucose target in the intervention group of > 120mg/dL (> 6.6mmol/L), or where intensive glucose control was only performed in the intraoperative period, and those where loss to follow-up exceeded 10% by hospital discharge. Primary endpoint: In-hospital mortality during index hospital admission. Secondary endpoints: mortality and survival at other timepoints, duration of invasive mechanical ventilation, vasoactive agents, and renal replacement therapy. A random effect Bayesian meta-analysis and hierarchical Bayesian models for individual patient data will be used. Discussion: This systematic review with aggregate and individual patient data will address the clinical question, ‘what is the best blood glucose target for critically ill patients overall?’ Protocol version 0.4 - 06/26/2023 PROSPERO registration: CRD42021278869

https://doi.org/10.5935/2965-2774.20230162-en

Development of monoclonal antibody based IgG and IgM ELISA for diagnosis of severe fever with thrombocytopenia syndrome virus infection
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Zhang, Mei

; Du, Yanhua Yang, Li Zhan, Lin Yang, Bin Huang, Xueyong Xu, Bianli Morita, Koichi Yu, Fuxun

Brazilian Journal of Infectious Diseases

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2022, Volume 26 N. 4 elocation 102386

Abstract: En | Text: En | PDF: En

ABSTRACT Introduction: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly emerged virus that poses a great threat to human health because of high fatality rate. Methods: To develop sensitive and specific sero-diagnostic systems for SFTSV infections, monoclonal antibodies (MAbs) against recombinant SFTSV nucleocapsid (rSFTSV-N) protein were developed by immunizing BALB/C mice with rSFTSV-N protein and fusing the spleen cells with SP2/0 myeloma cells. Three hybridoma cell lines secreting MAbs against rSFTSV-N were obtained. MAb based IgG sandwich enzyme linked immunosorbent assay (ELISA) and IgM capture ELISA systems were established by using the newly developed MAbs. One hundred fifteen clinical suspected SFTS patients serum samples were used to evaluate the newly established systems by comparing with the total antibody detecting sandwich ELISA system and indirect ELISA systems. Results: The MAbs based sandwich IgG ELISA was perfectly matched with that of the total antibody sandwich ELISA and the indirect IgG ELISA. IgM capture ELISA results perfectly matched with that of the total antibody sandwich ELISA while detecting eight additional positive samples missed by the indirect IgM ELISA. Conclusions: The MAbs against rSFTSV-N protein offer new tools for SFTSV studies and our newly developed MAb-based IgG and IgM capture ELISA systems would offer safe and useful tools for diagnosis of SFTS virus infections and epidemiological investigations.

https://doi.org/10.1016/j.bjid.2022.102386

Efficacy of Lagopsis supina to promote blood circulation, remove blood stasis, and block inflammation in a rat model of traumatic blood stasis
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Xu, Tingting

; Wang, Xiumei He, Zhongwei Yang, Li Wei, Rongrui Zhong, Guoyue He, Junwei

Brazilian Journal of Pharmaceutical Sciences

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2022, Volume 58 elocation e19359

Abstract: En | Text: En | PDF: En

Abstract Lagopsis supina (Steph) IK. Gal. was a traditional Chinese medicinal plant for promoting blood circulation and removing blood stasis (PBCRBS), anti-inflammatory and diuresis with little scientific validation. The aims of this study were first to evaluate the PBCRBS and anti-inflammatory effects of L. supina in a rat model of traumatic blood stasis (TBS). We demonstrated that an ethanolic extract of L. supina (LS, 460 mg/kg/d) possessed significant PBCRBS and remarkable inhibitory effect on inflammation cytokines, which were associated with renovated amount of the injured muscle fibers, alleviate the degree of the damaged tissue edema, decreased the number of inflammatory cells, increased the number of the capillary hyperplasia by hematoxylin and eosin (HE), as well as remarkably down-regulated ( p < 0.05 or p < 0.01) the levels of inflammation cytokines, including TNF-α, IL-6, IL-8, and IL-10 by ELISA. This finding provide a pharmacological basis and partial interpretation for the clinical application of L. supina, which has therapeutic properties for blood stasis syndrome (BSS) and inflammation-related diseases.

https://doi.org/10.1590/s2175-97902021000x32e19359

Comparison of the biochemical properties and enzymatic synthesis of theaflavins by soluble and membrane-bound polyphenol oxidases from tea (Camellia sinensis) leaves
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LIU, Yang

; CHEN, Qincao LIU, Dechun YANG, Li HU, Wei KUANG, Liuqing TENG, Jie LIU, Yong

Food Science and Technology

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2022, Volume 42 elocation e117321

Abstract: En | Text: En | PDF: En

Abstract Polyphenol oxidase (PPO) plays a key role in tea processing. It catalyzes the conversion of tea polyphenols into theaflavin and its derivatives. PPO was partially purified and characterized in both its soluble form (sPPO) and membrane-bound form (mPPO) from tea (Camellia sinensis) leaves. Both forms were purified by three-phase partitioning and membrane ultrafiltration. sPPO and mPPO showed high activity against diphenols as substrate and had the highest affinity for catechol and caffeic acid. The optimum temperatures and pH for enzyme activity were different. mPPO was more stable than sPPO in the acidic pH range. Among the chemical inhibitors studied, oxalic acid exerted the highest inhibitory effect on mPPO, whereas EDTA showed the highest inhibitory effect on sPPO. Both sPPO and mPPO showed similar enzymatic synthesis rates in the formation of theaflavin-3'-gallate and theaflavin-3,3'-gallate. At high concentrations of the substrate, the synthesis of theaflavins was inhibited due to the presence of high levels of ester catechins. However, mPPO showed stronger stability against inhibition by ester catechins than sPPO.

https://doi.org/10.1590/fst.117321

Analysis of antibiotic resistance phenotypes and genes of Escherichia coli from healthy swine in Guizhou, China
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Yu, Bo Zhang, Yanan Yang, Li Xu, Jinge Bu, Shijin

Onderstepoort Journal of Veterinary Research

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2021, Volume 88 N. 1 Pages 1 - 8

Abstract: En | Text: En | PDF: En

This study was carried out to investigate the resistance phenotypes and resistance genes of Escherichia coli from swine in Guizhou, China. A total of 47 E. coli strains isolated between 2013 and 2018 were tested using the Kirby-Bauer (K-B) method to verify their resistance to 19 common clinical antimicrobials. Five classes consisting of 29 resistance genes were detected using polymerase chain reaction. The status regarding extended-spectrum β-lactamase (ESBL) and the relationship between ESBL CTX-M-type β-lactamase genes and plasmid-mediated quinolone resistance (PMQR) genes were analysed. A total of 46 strains (97.9%) were found to be multidrug resistant. Amongst them, 27 strains (57.4%) were resistant to more than eight antimicrobials, and the maximum number of resistant antimicrobial agents was 16. Twenty antibiotic resistance genes were detected, including six β-lactamase genes blaTEM (74.5%), blaCTX-M-9G (29.8%), blaDHA (17.0%), blaCTX-M-1G (10.6%), blaSHV (8.5%), blaOXA (2.1%), five aminoglycoside-modifying enzyme genes aac(3′)-IV (93.6%), aadA1 (78.7%), aadA2 (76.6%), aac(3′)-II c (55.3%), aac(6′)-Ib (2.1%) and five amphenicol resistance genes floR (70.2%), cmlA (53.2%), cat2 (10.6%), cat1 (6.4%), cmlB (2.1%), three PMQR genes qnrS (55.3%), oqxA (53.2%), qepA (27.7%) and polypeptide resistance gene mcr-1 (40.4%). The detection rate of ESBL-positive strains was 80.9% (38/47) and ESBL TEM-type was the most abundant ESBLs. The percentage of the PMQR gene in blaCTX-M-positive strains was high, and the detection rate of blaCTX-M-9G was the highest in CTX-M type. It is clear that multiple drug resistant E. coli is common in healthy swine in this study. Extended-spectrum β-lactamase is very abundant in the E. coli strains isolated from swine and most of them are multiple compound genotypes.

https://doi.org/10.4102/ojvr.v88i1.1880 27 downloads

Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa
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Rong, Chengbo

; Song, Shuang

; Yang, Li

; Pan, Xuejiao

; Liu, Yu

; Wang, Shouxian

Ciência e Agrotecnologia

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2021, Volume 45 elocation e010921

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Pholiota adiposa é um cogumelo com excelentes propriedades nutricionais e medicinais. No entanto, o rendimento do corpo de frutificação deste é baixo e o potencial de cultivo comercial deste fungo é limitado. 279 linhagens cruzadas foram obtidas pelo cruzamento mono-mono de linhagens monocarióticas derivadas de P. adiposa HS5 e HS4. Enzimas ligninolíticas e taxas de crescimento micelial foram utilizadas como marcadores para a triagem das linhagens cruzadas, e 18 linhagens foram seleccionadas para análise posterior. A linhagem cruzada A10B4 apresentou o maior rendimento (ie 165,91 ± 12,56 g por sacola), o qual 31,34 g e, 74,48 g a mais do que as linhagens HS5 e HS4, respetivamente. O tempo de colonização micelial de A10B4 foi 25,18 ± 1,33 d, sendo 5,64 d menor que HS5. A10B4 foi caracterizado, por marcadores moleculares de repetição de sequência inter-simples. Foram observadas diferenças nos produtos de PCR de linhagens parentais e cruzadas. Portanto, a linhagem híbrida recém-desenvolvida A10B4 (Renominada P. adiposa HS54), exibindo alto rendimento, pode ser adequada para cultivo comercial.

ABSTRACT Pholiota adiposa is a mushroom with excellent nutritional and medicinal properties. However, fruiting body yields are low, and the commercial cultivation potential of this fungus is limited. Here, 279 crossbred strains were obtained by the mono-mono crossing of monokaryotic strains derived from P. adiposa HS5 and HS4. Laccase enzymes and mycelial growth rates were used as markers to screen the crossbred strains, and 18 strains were selected for further analysis. Crossbred strain A10B4 displayed the highest yield (i.e. 165.91 ± 12.56 g per bag), which was 31.34 g and 74.48 g more than that of strains HS5 and HS4, respectively. The mycelial colonization time of A10B4 was 25.18 ± 1.33 d, which was 5.64 d shorter than that of HS5. A10B4 was characterized by inter-simple sequence repeat molecular markers. Differences in PCR products from parental and crossbred strains were observed. Therefore, the newly developed hybrid strain A10B4, named P. adiposa HS54 exhibiting high yield, might be suitable for commercial cultivation.

https://doi.org/10.1590/1413-7054202145010921 74 downloads

Concordance of Chest CT and Nucleic Acid Testing in Diagnosing Coronavirus Disease Outside its District of Origin (Wuhan, China)
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Yang, Li-Qin

; Cao, Jin-Ming Chen, Tian-Wu Yang, Jian-Qiong Mu, Qi-Wen

Clinics

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2020, Volume 75 elocation e1910

Abstract: En | Text: En | PDF: En

OBJECTIVES: This study aimed to determine the concordance between CT and nucleic acid testing in diagnosing coronavirus disease (COVID-19) outside its district of origin (Wuhan, China). METHODS: Twenty-three consecutive patients with COVID-19, confirmed by nucleic acid testing, were enrolled from two designated hospitals outside the district of disease origin. We collected clinical, laboratory, and CT data and assessed the concordance between CT manifestations and nucleic acid test results by comparing the percentage of patients with and without abnormal CT findings. Furthermore, using Chi-square tests, we analyzed the differences in CT manifestations between patients with and without an exposure history or symptoms. RESULTS: Multiple ground-glass opacities (GGOs), with or without consolidation, were observed on the initial CT scans of 19 patients (82.6%), whereas the remaining 4 (17.4%) showed no CT abnormalities, indicating that the initial chest CT findings were not entirely concordant with the nucleic acid test results in diagnosing COVID-19. Among the latter 4 patients, we observed multiple GGOs with and without consolidation in 2 patients on the follow-up chest CT scans taken on days 7 and 14 after admission, respectively. The remaining 2 patients showed no abnormalities on the follow-up CT scans. Furthermore, abnormal CT findings were found more frequently in patients who had been exposed to COVID-19 in its district of origin than in those who had not been exposed and in symptomatic patients than in asymptomatic patients (all p<0.05). CONCLUSIONS: Patients with positive results on nucleic acid testing may or may not have the abnormal CT manifestations that are frequently found in symptomatic patients with a history of exposure to the district of COVID-19 origin.

https://doi.org/10.6061/clinics/2020/e1910 200 downloads

Role of low dose 256-slice CT perfusion imaging in predicting mediastinal lymph node metastasis of lung cancer
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Yang, Li

; Sun, Lu

; Liu, Jian

; Liu, Qiang

Revista da Associação Médica Brasileira

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jun 2019, Volume 65 N. 6 Pages 761 - 766

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO OBJETIVOS: A perfusão por TC objetiva (CTP) é uma técnica de imagem que pode ser usada para avaliar as alterações na microcirculação de tecidos tumorais. Nosso estudo teve como objetivo investigar o papel da CTP na predição de metástases em linfonodos mediastinais. MÉTODOS: Dados clínicos de 58 pacientes que receberam ressecção cirúrgica de câncer de pulmão e dissecção de linfonodos em nosso hospital de junho de 2012 a dezembro de 2014 foram coletados. Os pacientes foram divididos em grupo positivo para metástase linfonodal e grupo negativo para metástase linfonodal. Parâmetros de CTP incluindo pico de intensidade de realce (PEI) e valor de perfusão (PV), bem como volume de sangue (BV), foram comparados entre os dois grupos. A curva característica de operação do receptor (ROC) foi usada para predizer metástase linfonodal mediastinal. RESULTADOS: PV do grupo de linfonodos metastáticos positivos foi significativamente maior do que o grupo negativo de linfonodos metastáticos (p<0,001). A análise da curva ROC mostrou que a PV pode ser usada como um índice para predizer a metástase linfonodal mediastinal do câncer de pulmão. A sensibilidade e a especificidade da VP maior que 7,5ml · min-1 · ml-1 na predição de metástase linfonodal de câncer de pulmão foram de 78,3% e 91,4%, respectivamente. CONCLUSÃO: A VP de perfusão por TC de baixa dose pode ser usada como um índice para a predição de metástase linfonodal mediastinal de câncer de pulmão.

SUMMARY OBJECTIVE: CT perfusion (CTP) is an imaging technique that can be used to evaluate the changes in the microcirculation of tumor tissues. Our study aimed to investigate the role of CTP in predicting mediastinal lymph node metastasis. METHODS: Clinical data of 58 patients who received surgical resection of lung cancer and lymph node dissection in our hospital from June 2012 to December 2014 were collected. Patients were divided into a positive lymph node metastasis group and a negative lymph node metastasis group. Parameters of CTP, including peak enhancement intensity (PEI), perfusion value (PV), as well as blood volume (BV), were compared between the two groups. The receiver-operating characteristic (ROC) curve was used to predict mediastinal lymph node metastasis. RESULTS: The PV of the positive lymph node metastasis group was significantly higher than that of the negative group (p < 0.001). The ROC curve analysis showed that PV can be used as an index to predict mediastinal lymph node metastasis of lung cancer. The sensitivity and specificity of a PV greater than 7.5ml·min-1·ml-1 in predicting lymph node metastasis of lung cancer were 78.3 % and 91.4 %, respectively. CONCLUSION: The PV of low dose CT perfusion can be used as an index for predicting mediastinal lymph node metastasis of lung cancer.

https://doi.org/10.1590/1806-9282.65.6.761 841 downloads

10.

Expression of V2R and AQPs in the Renal Medulla of High-fat Nutritional Obesity of Rat
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Wang, Jinbao Wang, Pei Wang, Zhisheng Ran, Linwu Yang, Li Yang, Wen

International Journal of Morphology

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jun 2019, Volume 37 N. 2 Pages 459 - 465

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

SUMMARY: Recent evidence has indicated that adipose tissue produces bioactive substances that contribute to obesity-related kidney disease, altering the renal function and structure. Eight of the AQPs are expressed in the kidney, where several of them contribute to water absorption and maintenance of body water balance. In the study, we mainly examined the localization of AQP2, AQP3 and V2R in renal medulla of Normal Diet (ND) and High-fat Diet (HFD) of rats, respectively. In renal medulla of HFD, immunolight microscopy revealed weak expression of AQP2 at the apical plasma membrane and intracellular vesicles of principal cells of the IMCD and OMCD. AQP3 and V2R expression also observed a decrease in immunolabelling in the IMCD and OMCD. It was suggested that excess lipid accumulation may lead to lipotoxicity and may be the major driver of organ dysfunction such as water reabsorption dysfunction, which may be resulted from abnormal response of rphan G-protein-coupled receptors in kidney.

RESUMEN: La evidencia reciente ha indicado que el tejido adiposo produce sustancias bioactivas que contribuyen a la enfermedad renal relacionada con la obesidad, alterando la función y la estructura renal. Ocho de los AQP se expresan en el riñón, donde varios de ellos contribuyen a la absorción de agua y al mantenimiento del equilibrio hídrico corporal. En el estudio, examinamos principalmente la localización de AQP2, AQP3 y V2R en la médula renal de ratas con dieta normal (ND) y ratas con dieta alta en grasas (HFD). En la médula renal del grupo HFD, la microscopía electrónica de barrido reveló una expresión débil de AQP2 en la membrana plasmática apical y las vesículas intracelulares de las células principales de IMCD y OMCD. La expresión de AQP3 y V2R también observó una disminución en el inmunomarcador en IMCD y OMCD. Se sugiere que el exceso de acumulación de lípidos puede conducir a lipotoxicidad y ser el principal impulsor de la disfunción orgánica, como la disfunción de reabsorción de agua, que puede ser el resultado de la respuesta anormal de los receptores acoplados a proteína rphan G en el riñón.

11.

Comparison of retrograde flexible ureteroscopy and percutaneous nephrolithotomy in treating intermediate-size renal stones (2-3cm): a meta-analysis and systematic review
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Zewu, Zhu

; Cui, Yu Feng, Zeng Yang, Li Chen, Hequn

International braz j urol

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jan 2019, Volume 45 N. 1 Pages 10 - 22

Abstract: En | Text: En | PDF: En

ABSTRACT Purpose: To systematically assess the effectiveness and safety of retrograde flexible ureteroscopy (FURS) versus percutaneous nephrolithotomy (PCNL) in treating intermediate-size renal stones (2-3cm). Materials and Methods: PubMed, Ovid MEDLINE, Web of Science, Cochrane Central Register of Controlled Trials (CENTRAL) and EMBASE were researched to identify relevant studies up to May 2018. Article selection was performed through the search strategy based on Preferred Reporting Items for Systematic Reviews and Meta-Analyses criteria. The Newcastle-Ottawa Scale was applied to assess the methodological quality of case-control studies. Results: Six retrospective case-controlled trials were included for meta-analysis. The pooled results showed that PCNL was associated with a higher initial stone-free rate (SFR). After more complementary treatments, FURS provided a final SFR (OR: 1.69; 95% CI, 0.93-3.05; P = 0.08) comparable to that achieved by PCNL. PCNL was associated with a higher rate of overall intraoperative complications (OR: 1.48; 95% CI, 1.01-2.17; P = 0.04) and longer hospital stay (MD: 2.21 days; 95% CI, 1.11 to 3.30; P < 0.001). Subgroup analysis by Clavien-graded complication showed PCNL had significantly higher rates of minor complications (OR: 1.58; 95% CI, 1.04-2.41; P = 0.03). No significant difference was noted in major complications (OR: 1.14; 95% CI, 0.53-2.45; P = 0.73) or operative times (MD: −9.71 min; 95% CI, −22.02 to 2.60; P = 0.12). Conclusions: Multisession FURS is an effective and safe alternative to PCNL for the management of intermediate-size renal stones (2-3cm). It is advisable to balance the benefits and risks according to the individual characteristics of patients and to decide with patients by discussing the advantages and disadvantages of each procedure.

https://doi.org/10.1590/s1677-5538.ibju.2018.0510 1113 downloads

12.

Short-term effects of byproduct amendments and lime on physicochemical and microbiological properties of acidic soil from Jiaodong Peninsula of China
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Shen, Yuwen

; Lin, Haitao

; Song, Xiaozong Liu, Ping Bo, Luji Chen, Jianqiu Yang, Li

Ciência Rural

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2019, Volume 49 N. 6 elocation e20180098

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: As influências do emprego de subprodutos que contêm silício, cálcio, magnésio e potássio, sobre a qualidade do solo ácido na Península de Jiaodong da China, foram estudadas e comparadas com a da cal através do monitoramento das propriedades físico-químicas e atividades enzimáticas do solo ácido ao longo de um 120- período do dia. Os resultados mostraram que as alterações dos subprodutos e a cal aumentaram significativamente as atividades de pH, CE e enzimas do solo. As alterações dos subprodutos obviamente inibiram o carbono da biomassa microbiana e a respiração do solo, mas o solo tratado com cal apresentou um maior nível de emissão de CO2. Em comparação com as modificações do subproduto, o solo tratado tinha o maior pH, a atividade da peroxidase, a atividade da fenol oxidase e a atividade da invertease. Portanto, o cal pode ser uma escolha melhor em relação às alterações dos subprodutos para melhorar as propriedades químicas e biológicas do solo ácido na Península de Jiaodong da China.

ABSTRACT: The influences of byproduct amendments, containing silicon, calcium, magnesium and potassium, on acidic soil quality in Jiaodong Peninsula of China had been studied and compared with that of lime through monitoring physicochemical properties and enzyme activities of acidic soil over a 120-day period. Byproduct amendments (1125, 2250, 4500 and 9000 kg ha-1) and lime 2250 kg ha-1 was applied in the acidic soil. Results showed that both byproduct amendments and lime significantly increased the pH, EC and enzyme activities of soil. The by-product amendments inhibited microbial biomass carbon and soil respiration. Nevertheless, the lime-treated soil had a much more higher level of CO2 emission than the by-product amendments-treated soil. Compared to the by-product amendments-treated soil, the lime-treated soil had the higher pH, peroxidase activity, phenol oxidase activity and invertase activity. Therefore, lime might be a better choice over by-product amendments to improve chemical and biological properties of the acidic soil in Jiaodong Peninsula of China. For soils lacking available calcium and magnesium, the mixture of 4500 kg ha-1 amendments and 2250 kg ha-1 lime was recommend to treat the soil.

https://doi.org/10.1590/0103-8478cr20180098 695 downloads

13.

MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2
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Xie, Hui Hui Huan, Wen Ting Han, Jiang Qiong Ren, Wei Ru Yang, Li Hua

Biological Research

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2019, Volume 52 elocation 18

Abstract: En | Text: En | PDF: En

Abstract Background: MicroRNAs (miRNAs) have emerged as the critical modulators of the tumorigenesis and tumor progression. Methods: The levels of miR-663 in ovarian cancer cell lines and clinical tissues were detected using qRT-PCR assays. The Transwell invasion and wound healing assay were conducted to assess the roles of miR-663 in the migration and invasion of ovarian cancer cell in vitro. Rescue assays were carried out to confirm the contribution of tumor suppressor candidate 2 (TUSC2) in the aggressiveness of cancer cell which was regulated by miR-663. Results: The levels of miR-663 were up-regulated in ovarian cancer tissues in comparison with the corresponding normal tissues. Up-regulation of miR-663 increased the proliferation, colony formation, migration and invasion of ovarian cancer SKOV3 cell. Additional, over-expression of miR-663 increased the tumor growth of SKOV3 in xenograft model. Bioinformatics analysis and luciferase reporter assay identified that miR-663 decreased the level of TUSC2 via binding to the 3'-UTR of TUSC2 gene. Finally, the expression of TUSC2 was inversely associated with the level of miR–663 in ovarian carcinoma tissue and over-expression of TUSC2 inhibited the migration and invasion abilities of SKOV3 that was promoted by miR-663. Conclusion: Altogether, these results indicate that miR-663 acts as a potential tumor-promoting miRNA through targeting TUSC2 in ovarian cancer.

https://doi.org/10.1186/s40659-019-0219-6

14.

Accuracy of Percutaneous Core Biopsy in the Diagnosis of Small Renal Masses (≤4.0 cm): A Meta-analysis
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He, Qiqi Wang, Hanzhang Kenyon, Jonathan Liu, Guiming Yang, Li Tian, Junqiang Yue, Zhongjin Wang, Zhiping

International braz j urol

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feb 2015, Volume 41 N. 1 Pages 15 - 25

Abstract: En | Text: En | PDF: En

Objective To use meta-analysis to determine the accuracy of percutaneous core needle biopsy in the diagnosis of small renal masses (SMRs≤4.0 cm). Materials and Methods Studies were identified by searching PubMed, Embase, and the Cochrane Library database up to March 2013. Two of the authors independently assessed the study quality using QUADAS-2 tool and extracted data that met the inclusion criteria. The sensitivity, specificity, likelihood ratios, diagnostic odds ratio (DOR) and also summary receiver operating characteristic (SROC) curve were investigated and draw. Deek’s funnel plot was used to evaluate the publication bias. Result A total of 9 studies with 788 patients (803 biopsies) were included. Failed biopsies without repeated or aborted from follow-up/surgery result were excluded (232 patients and 353 biopsies). For all cases, the pooled sensitivity was 94.0% (95% CI: 91.0%, 95.0%), the pooled positive likelihood was 22.57 (95% CI: 9.20-55.34), the pooled negative likelihood was 0.09 (95% CI: 0.06-0.13), the pooled DOR was 296.52(95% CI: 99. 42-884.38). The area under the curve of SROC analysis was 0.959±0.0254. Conclusion Imaging-guided percutaneous core needle biopsy of small renal masses (SMRs≤4.0 cm) is highly accurate to malignant tumor diagnosis with unknown metastatic status and could be offered to some patients after clinic judgment prior to surgical intervention consideration.

https://doi.org/10.1590/S1677-5538.IBJU.2015.01.04 1487 downloads

15.

Mesenteric lymph reperfusion after superior mesenteric artery occlusion shock exacerbates endotoxin translocation in brain
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Zhao, Zi-gang Yang, Li-na Zhao, Yong-quan Niu, Chun-yu

Acta Cirúrgica Brasileira

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jun 2014, Volume 29 N. 6 Pages 359 - 364

Abstract: En | Text: En | PDF: En

PURPOSE: To determine the role of mesenteric lymph reperfusion (MLR) on endotoxin translocation in brain to discuss the mechanism of brain injury subjected to superior mesenteric artery occlusion (SMAO) shock. METHODS: Twenty-four rats were randomly assigned to MLR, SMAO, MLR+SMAO and sham groups. MLR was performed by clamping the mesenteric lymph duct (MLD) for 1 h and then allowing reperfusion for 2 h in the MLR group; SMAO involved clamping the superior mesenteric artery (SMA) for 1 h, followed by reperfusion for 2 h in the SMAO group; occlusion of both the SMA and MLD for 1 h was followed by reperfusion for 2 h in the MLR+SMAO group rats. RESULTS: SMAO shock induced severe increased levels of the endotoxin, lipopolysaccharide receptor, lipopolysaccharide-binding protein, intercellular adhesion molecule-1 and tumor necrosis factor-α. Concurrently, MLR after SMAO shock further aggravates these deleterious effects. CONCLUSION: Mesenteric lymph reperfusion exacerbated the endotoxin translocation in brain; thereby increased inflammatory response occurred, suggesting that the intestinal lymph pathway plays an important role in the brain injury after superior mesenteric artery occlusion shock.

https://doi.org/10.1590/S0102-86502014000600002 1726 downloads

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