ABSTRACT Objective: To investigate the impact of impaired pulmonary function on patient-centered outcomes after hospital discharge due to severe COVID-19 in patients without preexisting respiratory disease. Methods: This is an ongoing prospective cohort study evaluating patients (> 18 years of age) 2-6 months after hospital discharge due to severe COVID-19. Respiratory symptoms, health-related quality of life, lung function, and the six-minute walk test were assessed. A restrictive ventilatory defect was defined as TLC below the lower limit of normal, as assessed by plethysmography. Chest CT scans performed during hospitalization were scored for the presence and extent of parenchymal abnormalities. Results: At a mean follow-up of 17.2 ± 5.9 weeks after the diagnosis of COVID-19, 120 patients were assessed. Of those, 23 (19.2%) reported preexisting chronic respiratory diseases and presented with worse lung function and exertional dyspnea at the follow-up visit in comparison with their counterparts. When we excluded the 23 patients with preexisting respiratory disease plus another 2 patients without lung volume measurements, a restrictive ventilatory defect was observed in 42/95 patients (44%). This subgroup of patients (52.4% of whom were male; mean age, 53.9 ± 11.3 years) showed reduced resting gas exchange efficiency (DLCO), increased daily-life dyspnea, increased exertional dyspnea and oxygen desaturation, and reduced health-related quality of life in comparison with those without reduced TLC (50.9% of whom were male; mean age, 58.4 ± 11.3 years). Intensive care need and higher chest CT scores were associated with a subsequent restrictive ventilatory defect. Conclusions: The presence of a restrictive ventilatory defect approximately 4 months after severe COVID-19 in patients without prior respiratory comorbidities implies worse clinical outcomes. Objective patientcentered patient centered COVID19 COVID 19 COVID-1 Methods > ( 1 age 26 6 2- COVID19. 19. symptoms healthrelated health related sixminute six minute normal plethysmography abnormalities Results followup follow up 172 17 17. 59 5 9 5. COVID19, 19, 12 19.2% 192 (19.2% counterparts measurements 4295 42 95 42/9 44%. 44 44% . (44%) 52.4% 524 52 (52.4 male 539 53 53. 113 11 3 11. DLCO, DLCO , (DLCO) dailylife daily desaturation 50.9% 509 50 (50.9 584 58 58. years. Conclusions COVID1 COVID- 19.2 (19.2 429 42/ (44% 52.4 (52. (DLCO 50.9 (50. (19. (44 52. (52 50. (50 (19 (4 (5 (1

RESUMO Objetivo: Investigar o impacto do comprometimento da função pulmonar nos desfechos centrados no paciente após a alta hospitalar em pacientes sem doenças respiratórias preexistentes que foram hospitalizados em virtude de COVID-19 grave. Métodos: Trata-se de um estudo prospectivo de coorte em andamento, no qual pacientes com COVID-19 grave (com idade > 18 anos) são avaliados 2-6 meses depois da alta hospitalar. Avaliamos os sintomas respiratórios, a qualidade de vida relacionada à saúde, a função pulmonar e a distância percorrida no teste de caminhada de seis minutos. A definição de distúrbio ventilatório restritivo foi CPT abaixo do limite inferior da normalidade na pletismografia. As imagens de TC de tórax realizadas durante a hospitalização foram avaliadas quanto à presença e extensão de alterações parenquimatosas. Resultados: Em média 17,2 ± 5,9 semanas depois do diagnóstico de COVID-19, foram avaliados 120 pacientes. Destes, 23 (19,2%) relataram doenças respiratórias crônicas preexistentes e apresentaram pior função pulmonar e maior dispneia aos esforços na consulta de acompanhamento quando comparados aos outros participantes. Quando excluímos os 23 pacientes com doenças respiratórias preexistentes e mais 2 pacientes (sem medidas de volumes pulmonares), observamos distúrbio ventilatório restritivo em 42/95 pacientes (44%). Esse subgrupo de pacientes (52,4% dos quais eram do sexo masculino, com média de idade de 53,9 ± 11,3 anos) apresentou menor eficiência das trocas gasosas (DLCO), maior dispneia na vida diária e dessaturação de oxigênio ao exercício e redução da qualidade de vida relacionada à saúde em comparação com aqueles sem redução da CPT (50,9% dos quais eram do sexo masculino, com média de idade de 58,4 ± 11,3 anos). A necessidade de terapia intensiva e pontuações mais altas no escore de alterações parenquimatosas na TC de tórax apresentaram relação com distúrbio ventilatório restritivo subsequente. Conclusões: A presença de distúrbio ventilatório restritivo aproximadamente 4 meses depois da COVID-19 grave em pacientes sem comorbidades respiratórias prévias implica piores desfechos clínicos. Objetivo COVID19 COVID 19 COVID-1 Métodos Tratase Trata se andamento 1 anos 26 6 2- respiratórios minutos pletismografia Resultados 172 17 17, 59 5 9 5, COVID19, 19, 12 Destes 19,2% 192 (19,2% participantes pulmonares, pulmonares , pulmonares) 4295 42 95 42/9 44%. 44 44% . (44%) 52,4% 524 52 (52,4 masculino 539 53 53, 113 11 3 11, DLCO, DLCO (DLCO) 50,9% 509 50 (50,9 584 58 58, anos. subsequente Conclusões clínicos COVID1 COVID- 19,2 (19,2 429 42/ (44% 52,4 (52, (DLCO 50,9 (50, (19, (44 52, (52 50, (50 (19 (4 (5 (1 (

RESUMO: Este estudo foi realizado para determinar a relação ideal de treonina:lisina digestíveis em rações com baixo nível de proteína bruta para galinhas poedeiras leves de 24 a 40 semanas de idade. Utilizaram-se 240 poedeiras Hy Line W-36 distribuídas em delineamento inteiramente casualizado, com cinco relações treonina:lisina digestíveis (60; 67; 74; 81 e 88%), oito repetições e seis aves por unidade experimental. As relações treonina:lisina digestíveis não influenciaram o consumo de ração e de lisina; peso médio dos ovos e seus componentes; ganho de peso e peso corporal final das aves, mas houve efeito linear para o consumo de treonina. O modelo Linear Response Plateau (LRP) foi o que melhor representou a distribuição dos dados para taxa de postura; massa de ovos; eficiência de utilização da lisina por massa; conversão alimentar por massa e por dúzia de ovos; e balanço de nitrogênio. Os platôs ocorrem nos níveis de treonina de 0.61; 0.580; 0.614; 0.599; 0.599 e 0.643%, que corresponde às relações treonina:lisina digestíveis de 80; 75; 80; 78; 78 e 83%, respectivamente. A relação ideal de treonina:lisina digestíveis em rações para galinhas poedeiras leves de 24 a 40 semanas de idade é de 80%.

ABSTRACT: This trial was performed to determine the dietary digestible threonine-to-lysine (dig. Thr-to-Lys) ideal ratio for laying hens fed with low-protein diets from 24 to 40 weeks of age. At 24 weeks of age, two hundred forty Hy Line W-36 laying hens were randomly assigned to five treatments, represented by five dig Thr-to-Lys ratios (60; 67; 74; 81 and 88%), with eight replicates each one with six hens per experimental unit. Digestible Thr-to-Lys did not affect laying hen performance and egg quality, except for digestible Threonine intake, which showed linear increase as dig. Thr-to-Lys ratios increased. Linear broken-line model was chosen to analyze data according to lack of fit. According to linear broken-linen estimates, digestible Threonine ideal levels for optimum egg production, egg mass, feed efficiency of lysine utilization for egg mass, feed conversion per egg mass and dozens of eggs and nitrogen balance were 0.610; 0.580; 0.614; 0.599; 0.599 and 0.643%, which correspond respectively to dig Thr-to-Lys ratios of 80; 75; 80; 78; 78 and 83%. The digestible threonine-to-lysine ideal ratio for laying hens from 24 to 40 weeks of age is 80%.

RESUMO: Com o objetivo de avaliar o efeito dos níveis de energia metabolizável (EM) sobre o desempenho de codornas de corte de 15 a 35 dias de idade, foi conduzido um experimento com 560 aves aos 15 dias de idade, distribuídas em delineamento inteiramente casualizado com cinco tratamentos (2.850; 2.950; 3.050; 3.150 e 3.250kcal de EM kg-1 de ração), oito repetições com 14 aves por unidade experimental. Verificou-se redução linear (P<0,01) no consumo de ração, de proteína, de lisina e na conversão alimentar, com o aumento dos níveis de EM da ração. O consumo de energia metabolizável, o peso corporal, o ganho de peso, viabilidade das aves não foram influenciados (P>0,05) pelos níveis de EM utilizados. Os níveis de EM das dietas não influenciaram (P>0,05) a matéria seca, o teor de umidade e a proteína nas carcaças. Foi observado efeito quadrático (P<0,04) dos níveis de EM sobre o teor de gordura nas carcaças. Conclui-se que o nível de EM de 3.250kcal kg-1 de ração, correspondendo à relação de energia metabolizável:proteína bruta de 139,24, possibilita melhor desempenho das codornas de corte.

ABSTRACT: This trial was carried out to evaluate the effects of dietetic metabolizable energy levels on performance and carcass traits of meat quails from 15 to 35 days old. Five hundred sixty, 15-d old, meat quails were randomly assigned to five treatments (2.850; 2.950; 3.050; 3.150 e 3.250kcal of ME kg-1 of diet), with eight replicates and fourteen birds per experimental unit. Feed intake, protein and lysine intake and feed conversion decreased linearly as the metabolizable energy content of diets increased (P<0.01), whereas metabolizable energy intake, body weight, weight gain and viability were not affected (P>0.05) by the treatments. Diets did not influence (P>0.05) carcass traits as dry matter, moisture and protein content in carcass. However a quadratic effect (P<0.04) were observed on carcass fat content. Based on these results, the adequate metabolizable energy level to ensure better meat quails' growth is 3.250kcal of ME kg-1 diet, that corresponds to a metabolizable energy: crude protein ratio of 139,24.

RESUMO Este estudo foi realizado para determinar a relação ideal de treonina:lisina digestíveis para poedeiras leves no período de 42 a 58 semanas de idade. Foram utilizadas 240 poedeiras Hy Line W-36 com 42 semanas de idade durante 16 semanas. Os tratamentos foram distribuídos em delineamento inteiramente casualizado, com oito repetições e seis aves por unidade experimental. As dietas experimentais foram formuladas com cinco níveis de treonina digestível (0,461; 0,515; 0,569; 0,622 e 0,676%) proporcionando as cinco relações treonina:lisina digestíveis (60; 67; 74; 81 e 88%) estudadas. As variáveis analisadas foram: produção de ovos; consumo de ração, de lisina e de treonina; massa e peso médio dos ovos; eficiências de utilização da lisina por massa e ovos produzidos; conversões alimentares por massa e dúzia de ovos; pesos médios de: gema, albúmen e de casca; ganho de peso; peso corporal final das aves; e balanço de nitrogênio. As relações treonina:lisina digestíveis na dieta não influenciaram as variáveis analisadas, exceto o consumo de treonina que apresentou efeito linear. A relação ideal de treonina:lisina digestíveis na dieta para galinhas poedeiras leves de 42 a 58 semanas de idade é de 60%, que corresponde ao nível de 0,461% de treonina digestível na dieta e ao consumo diário de treonina digestível de 446mg/ave.

SUMMARY This study was conducted to determine the ideal ratio of digestible threonine: lysine in diets for laying hens at 42 to 58 weeks of age. Two hundred forty Hy Line W-36 laying hens were used at 42 weeks of age for 16 weeks. The treatments were distributed in a completely randomized design with eight replicates and six birds each. The experimental diets were formulated with five levels of digestible threonine (0.461; 0.515; 0.569; 0.622 and 0.676%) providing the five ratio of digestible threonine: lysine (60; 67; 74; 81 and 88%) studied. The variables analyzed were: egg production; feed intake; lysine intake; threonine intake; mass egg; average egg weight; lysine use efficiencies by mass and produced eggs; feed conversion per mass and dozen eggs; Average weights yolk, albumen and shell; weight gain and final body weights of birds; and nitrogen balance. The digestible threonine: lysine ratio in the diet did not influence the analyzed variables except consumption of threonine which showed a linear effect. The ideal ratio of digestible threonine: lysine in diets for white laying hens at 42 to 58 weeks of age is 60%, which corresponds to the level of 0.461% of digestible threonine in the diet and daily intake of digestible threonine 446mg/bird.

O objetivo com este trabalho foi determinar a exigência nutricional de lisina digestível para galinhas poedeiras leves em idade avançada. Foram utilizadas 288 galinhas poedeiras Hy Line W-36 com 60 semanas de idade, distribuídas em delineamento inteiramente casualizado, com seis níveis de lisina digestível (6,0; 6,6; 7,2; 7,8; 8,4 e 9,0g kg-1), oito repetições e seis aves por unidade experimental. Os níveis de lisina digestível na ração influenciaram de forma linear o consumo de lisina e a eficiência de utilização da lisina. Para a produção e massa de ovos, os platôs ocorreram a partir dos níveis de 6,57g kg-1 e de 7,10g kg-1 de lisina digestível, respectivamente. Houve efeito quadrático dos níveis de lisina sobre o peso de gema, com o nível estimado em 7,6g kg-1de lisina digestível. A exigência de lisina digestível de galinhas poedeiras leves no período de 60 a 76 semanas de idade é de 7,10g kg-1 de ração, correspondendo ao consumo diário de 690mg ave-1 de lisina digestível.

The objective of this research was to determine the nutritional requirement of digestible lysine for white laying hens in old age. Two hundred eighty-eight Hy Line W-36 laying hens at 60 weeks of age were distributed in a completely randomized design, with six levels of digestible lysine (6.0; 6.6; 7.2; 7.8; 8.4 and 9.0g kg-1) and eight replications with six birds per experimental unit. There was linear effect of lysine levels on lysine intake and efficiency of utilization of lysine. For the production and egg mass, the levels of digestible lysine in the diet from which the plateaus occurred were of 6.57g kg-1 and 7.10g kg-1, respectively. There was quadratic effect of lysine levels on yolk weight, with the estimated level of 7.6g kg-1 of digestible lysine. The digestible lysine requirement of white laying hens from 60 to 76 weeks of age is 7.10g kg-1 in diet, corresponding to daily intake of 690mg bird-1 of digestible lysine.

Two hundred fifty-two Hy-Line W36 laying hens were allotted in a randomized block design with 6 treatments, 7 replicates and 6 hens per experimental unit in order to determine the ideal digestible isoleucine: digestible lysine (Ile:Lys) ratio for laying hens aged 24-40 weeks. Experimental diets contained 5.92, 6.33, 6.73, 7.14, 7.54 and 7.95 g/kg of digestible isoleucine, resulting in different Ile:Lys ratios (0.73:1, 0.78:1, 0.83:1, 0.88:1, 0.93:1 and 0.98:1). Experimental diets were isonitrogenous, with different dietary contents of glutamic acid, and made isocaloric by adjusting the levels of cornstarch. All essential amino acids were provided proportionally to digestible lysine. Feed intake, egg production, egg weight, egg mass, feed conversion ratio per egg mass, feed conversion ratio per dozen eggs, albumen, yolk and eggshell content were assessed. Feed intake, egg production, egg mass and feed conversion ratio were influenced by Ile: Lys ratios quadratically. The egg weight was not affected by Ile: Lys ratio. No differences were observed in the egg quality according to Ile: Lys ratios. The data indicates that the optimum digestible isoleucine: digestible lysine ratio for laying hens aged 24-40 week is 0.84:1, corresponding to the consumption of 681 mg of isoleucine and 811 mg of lysine/hen day-1.

Com o objetivo de avaliar a qualidade de ovos de codornas japonesas tratados superficialmente, através de imersão em óleo mineral ou solução de própolis, foram conduzidos experimentos simultâneos em dois ambientes, sem refrigeração (Experimento 1) e com refrigeração (Experimento 2). Para cada experimento, foram selecionados 360 ovos íntegros distribuídos em delineamento inteiramente casualizado em esquema fatorial 3 x 5 (ovos não tratados; ovos imersos em solução de própolis e ovos imersos em óleo mineral, avaliados durante cinco tempos de armazenamento: 7; 14; 21; 28 e 35 dias), cada tratamento foi composto por seis repetições de quatro ovos cada. Os ovos apresentaram aumento da perda de peso, diminuição do peso específico e aumento do peso e do pH da gema, além de redução do pH do albúmen e de seu respectivo peso com o avançar do tempo de estocagem na ausência de refrigeração (Experimento 1), independente se receberam ou não tratamento na casca. Entretanto, o tratamento superficial com óleo mineral promoveu proteção contra essa perda progressiva de peso e diminuição do peso específico, tornando-as menos intensas com o avançar do período de estocagem sem refrigeração. No Experimento 2, a imersão dos ovos em óleo mineral conferiu menor perda de peso, maior peso específico, maior peso de gema e de albúmen durante o armazenamento sob refrigeração. A qualidade de ovos de codornas submetidos a tratamento superficial da casca por imersão em óleo mineral é preservada por até cinco semanas em diferentes ambientes, sem e com refrigeração.

With the aim of evaluating the quality of eggs of Japanese quail treated superficially, by immersion in mineral oil or propolis solution, simultaneous experiments were conducted in two environments, without refrigeration (Experiment 1) and with refrigeration (Experiment 2). For each experiment, were selected 360 intact eggs distributed in a completely randomized design in factorial 3 x 5 (untreated eggs; eggs immersed in propolis or eggs immersed in mineral oil, evaluated for five storage times: 7; 14; 21; 28 and 35 days), each treatment consisted of four replicates of six eggs each. The eggs had increased weight loss, decrease in specific gravity and growth the weight and the pH of the yolk, besides the reduced the pH of the albumen and their respective weight with advancing storage time in the absence of cooling (Experiment 1), whether treated or not in the shell. However, the surface treatment with mineral oil promoted protection against progressive loss of weight and decreased specific gravity, making them less intense with advancing the storage period without refrigeration. In the Experiment 2, the immersion of eggs in mineral oil promoted less weight loss, higher specific weight, greater weight of the yolk and the albumen during storage under refrigeration. The quality of quail eggs submitted of treatment superficial on shell by immersion in mineral oil is preserved for up to five weeks under different environments, with and without refrigeration.

The experiment was conducted to determine the nutritional requirement of calcium and the best calcium:available phosphorus ratio for commercial layers at the post-laying peak. A total of 324 Hy-Line W-36 laying hens were utilized in the period from 42 to 58 weeks of age, distributed in a completely randomized design in a 3 × 3 factorial arrangement, composed of three levels of calcium (39, 42 and 45 g/kg) and three calcium:phosphorus ratios (12.12:1; 10.53:1; and 9.30:1), totaling nine treatments with six replications and six birds per experimental unit. There was no significant effect from the calcium levels × calcium:phosphorus ratio interaction for any of the variables studied. The calcium levels and the calcium:phosphorus ratios did not affect the variables performance or egg and bone quality. At the evaluation of the calcium:phosphorus balance, as the levels of calcium of the diet were raised, the intake of calcium and phosphorus and the contents of mineral matter and calcium in the excreta increased linearly, and the retention of calcium by birds decreased linearly. With the reduction of the calcium:phosphorus ratios of the diet, intake, retention and excretion of phosphorus by layers increased. Diets containing calcium at 39 g/kg and a calcium:phosphorus ratio of 12.12:1, corresponding to an increase in calcium of 3.51 g/bird/day and available phosphorus of 289 mg/bird/day, meet the requirements of calcium and available phosphorus of white egg layers in the period from 42 to 58 weeks of age.

Two hundred and fifty-two Hy-Line W36 laying hens were allotted in a completely randomized design with 6 treatments, 7 replicates and 6 hens per experimental unit in order to determine the ideal ratio of isoleucine (Ile) in relation to lysine (Lys) to laying hens aged 42-58 weeks. Experimental diets contained digestible Ile at different levels, resulting in different Ile:Lys ratios (0.73:1; 0.78:1; 0.83:1; 0.88:1; 0.93:1 and 0.98:1). A basal diet was formulated to provide Isoleucine in levels below recommendations. This diet was supplemented with L-isoleucine to make up the 6 diets. Each diet was made isonitrogenous by varying the dietary contents of glutamic acid and isocaloric by adjusting the contents of cornstarch. All essential amino acids were provided proportionally to lysine. Egg production, egg weight, egg mass, feed conversion ratio, albumen, yolk and eggshell contents were recorded and compiled at every 28-day period. No differences were observed in the performance over a wide range of dietary isoleucine concentrations from 5.76 to 7.73 g/kg corresponding to 0.73:1 to 0.98:1 Ile:Lys ratios. The lowest Ile:Lys ratio (0.73:1) was sufficient to ensure satisfactory performance of birds, corresponding to the consumption of 534 mg of isoleucine and 731 mg of lysine/day.