ABSTRACT It is common for vineyards in regions of high altitude to have high organic matter content in soil. Furthermore, reduced miner alization of organic matter and decomposing residues is expected because of the low temperatures. Thus, soils may not provide enough mineral nitrogen (N) to meet the needs of the grapevines and so the application of N sources is necessary, but may affect yield and must composition. The study aimed to evaluate yield and must composition of grapevines subjected to N application in soil with high organic matter content and the relationship with temperature and rainfall. The study was conducted in crops sea sons 2011/12, 2012/13 and 2013/14 in a vineyard located in the city of Água Doce, state of Santa Catarina, southern Brazil. The treatments consisted of N doses (0, 20, 40, 80 and 120 kg N ha-1) supplied via urea. Leaves opposite to the cluster were collected at full flowering and at veraison. Afterwards, the leaves were dried, ground and submitted to N analysis. Parameters of yield and must composition were evaluated. The dose of maximum technical efficiency in Cabernet Sauvignon grapevines was reached with 46 kg N ha-1 year-1 in crop season 2012/13. Leaf N content was a sensitive parameter to the application of N doses in the soil and underwent seasonal variation in relation to rainfall distribution. The N doses reduced total soluble solids (TSS) contents in the must. Based on principal component analysis, we found a correlation between total N content in leaf and accumulated rainfall, as well as between yield and temperature.

RESUMEN Los viñedos ubicados en regiones altas poseen suelos con alto contenido de materia orgánica. Sin embargo, debido a la baja temperatura se espera menor mineralización de la materia orgánica. De esta forma, los suelos pueden proporcionar menos cantidad de nitrógeno (N) mineral, respecto a los requerimientos de la vid. Por eso, es necesaria la aplicación de nitrógeno que puede afectar la productividad de la vid y la composición de la uva. Este estudio tuvo como objetivo evaluar la productividad y la composición del mosto de la vid sometido a la aplicación de N en suelos con alto contenido de materia orgánica y la relación con la temperatura y la pluviometría. El estudio fue realizado durante las campañas de los años 2011/12, 2012/13 y 2013/14, en un viñedo ubicado en el municipio de Agua Doce, Santa Catarina, región sur de Brasil. Los tratamientos fueron diferentes dosis de N (0, 20, 40, 80 e 120 kg N ha-1), suministrados con urea. La dosis de máxima eficiencia técnica en la vid Cabernet Sauvignon fue alcanzada con 46 kg N ha-1 año-1 en suelos con alto contenido de materia orgánica. El contenido de nitrógeno en las hojas fue un parámetro sensible a la aplicación de dosis de N en el suelo es sensible a la distribución de las lluvias. El N reduce el contenido de sólidos solubles totales en el mosto. Con base en los principales componentes analizados, los resultados indican una correlación entre el contenido de nitrógeno total en las hojas y la precipitación acumulada, así como entre los rendimientos y la temperatura.

ABSTRACT Applications for long periods of organic animal waste as a source of nutrients to crops can contaminate the soil and the surface and subsurface waters. This study aimed to evaluate how the utilization of organic waste, after successive applications, affects P fractions in the soil and consequently environmental contamination. In an experiment conducted for 8 years, totaling 12 applications with pig slurry manure (PSM), liquid cattle manure (LCM), swine deep bedding (SDB), and mineral fertilizer (NPK), 460; 505; 1.111; and 535 kg P∙ha−1 were added to the soil through PSM, LCM, SDB, and NPK, respectively. In September 2012, soil samples were collected in the layers 0.0 – 0.04; 0.04 – 0.08; 0.08 – 0.12; 0.12 – 0.16; 0.16 – 0.20 m, prepared and subjected to a sequential chemical fractionation of P. The 12 applications of pig slurry, liquid cattle manure, swine deep bedding and NPK fertilizer for eight years (urea + triple superphosphate + potassium chloride) provided accumulations of P fractions in the soil that were above the necessary for a proper nutrition to plants considering the available P fractions (AER + organic NaHCO3 + inorganic NaHCO3) in the soil. In addition, they showed that the use of swine deep bedding, followed by pig slurry, means higher levels of available P fractions in the soil. In this sense, the establishment of a dosage of organic waste to meet the nitrogen need of crops is not an environmentally appropriate parameter. It may cause P accumulation in the soil, increased absorption ability of plants and soil support, contributing to environmental contamination.

A adição de doses de dejetos líquidos de suínos e o cultivo de plantas podem modificar o teor de fósforo (P) no solo e a distribuição de suas espécies químicas na solução. Este trabalho objetivou avaliar o teor de P total, disponível e na solução e, a distribuição das suas espécies químicas na solução, em um solo submetido à aplicação de doses de dejetos líquidos de suínos por longo tempo e cultivo de plantas. O trabalho foi realizado em colunas de solo com estrutura indeformada, derivadas de um experimento conduzido durante oito anos, na unidade experimental da Universidade Federal de Santa Maria (UFSM), em Santa Maria, RS. O solo foi um Argissolo Vermelho distrófico arênico, submetido a aplicações de 0, 20, 40 e 80 m3 ha-1 de dejetos líquidos de suínos. Foram realizadas coletas de solos antes e após os cultivos de aveia preta e milho em casa de vegetação, nas camadas de 0-5, 5-10, 10-20, 20-30, 30-40 e 40-60 cm, para determinar os teores de P disponíveis e totais e a extração da solução. Na solução, foi determinada a concentração dos principais cátions, ânions, carbono orgânico dissolvido (COD) e o pH. A distribuição das espécies químicas de P foi obtida pelo software Visual Minteq. As 21 aplicações de dejetos líquidos de suínos aumentaram o teor de P total no solo até 40 cm e o teor de P extraído por Mehlich-1 e na solução até 30 cm de profundidade. As sucessivas aplicações de dejetos líquidos de suínos alteraram o equilíbrio entre a fase sólida e a líquida do solo nas camadas superficiais, aumentando a proporção do teor total de P do solo presente na solução, além de alterar as espécies químicas na solução, reduzindo a porcentagem complexada com Al e elevando a complexada com Ca e Mg nas camadas de 0-5 e 5-10 cm. O cultivo de aveia preta e milho elevou o pH da solução, consequentemente aumentando a proporção de HPO42- e diminuindo a espécie H2PO4-.

The application of pig slurry rates and plant cultivation can modify the soil phosphorus (P) content and distribution of chemical species in solution. The purpose of this study was to evaluate the total P, available P and P in solution, and the distribution of chemical P species in solution, in a soil under longstanding pig slurry applications and crop cultivation. The study was carried out in soil columns with undisturbed structure, collected in an experiment conducted for eight years in the experimental unit of the Universidade Federal de Santa Maria (UFSM), Santa Maria (RS). The soil was an Argissolo Vermelho distrófico arênico (Typic Hapludalf), subjected to applications of 0, 20, 40, and 80 m3 ha-1 pig slurry. Soil samples were collected from the layers 0-5, 5-10, 10-20, 20-30, 30-40, and 40-60 cm, before and after black oat and maize grown in a greenhouse, for the determination of available P, total P and P in the soil solution. In the solution, the concentration of the major cations, anions, dissolved organic carbon (DOC), and pH were determined. The distribution of chemical P species was determined by software Visual Minteq. The 21 pig slurry applications increased the total P content in the soil to a depth of 40 cm, and the P extracted by Mehlich-1 and from the solution to a depth of 30 cm. Successive applications of pig slurry changed the balance between the solid and liquid phases in the surface soil layers, increasing the proportion of the total amount of P present in the soil solution, aside from changing the chemical species in the solution, reducing the percentage complexed with Al and increasing the one complexed with Ca and Mg in the layers 0-5 and 5-10 cm. Black oat and maize cultivation increased pH in the solution, thereby increasing the proportion of HPO42- and reducing H2PO4- species.