Abstract Background Skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi are characterized by lymphohistiocytic inflammatory infiltrate associated with epithelioid granuloma and scarce parasitism. However, the in situ cellular immune response of these patients is unclear. Therefore, the aim of the present study was to characterize the cellular immune response in the skin lesions of patients affected by NUCL. Methods Twenty biopsies were processed by immunohistochemistry using primary antibodies to T lymphocytes (CD4, CD8), NK cells, B lymphocytes, macrophages, nitric oxide synthase and interferon-gamma. Results Immunohistochemistry revealed higher expression of all cellular types and molecules (IFN-γ, iNOS) in the dermis of diseased skin compared to the skin of healthy individuals (p < 0.05). Morphometric analysis performed in the skin lesions sections showed the predominance of CD8+ T lymphocytes in the mononuclear infiltrate, followed by macrophages, mostly iNOS+, a response that could be mediated by IFN-γ. Conclusion Our study improves knowledge of the cellular immune response in non-ulcerated or atypical cutaneous leishmaniasis caused by L. (L.) infantum chagasi in Central America and pointed to the pivotal participation of CD8+ T lymphocytes in the host defense mechanisms against the parasite in patients with NUCL.

ABSTRACT The main goal of this manuscript is to introduce a discrete dynamical system defined by symmetric matrices and a real parameter. By construction, we rediscovery the Power Iteration Method from the Projected Gradient Method. Convergence of the discrete dynamical system solution is established. Finally, we consider two applications, the first one consists in find a solution of non linear equation problem and the other one consists in verifies the optimality conditions when we solve quadratic optimization problems over linear equality constraints.

In this work, we study coerciveness notions and their implications to existence conditions. We start with the presentation of classical ideas of coerciveness in the framework of Optimization Theory, and, then, using a classical technical result, introduced by Ky Fan in 1961, we extend these ideas first to Optimization Problems and then to Equilibrium Problems. We point out the importance of related conditions to the introduced coerciveness notion in order to obtain existence results for Equilibrium Problems, without using monotonicity or generalized monotonicity assumptions.