Abstract We report a viral metagenomic analysis of fecal samples from Bradypus crinitus (Pilosa: Bradypodidae), a recently described sloth species that occurs in the Atlantic Forest of Espírito Santo and Rio de Janeiro states, Southeast Brazil. Through Illumina sequencing, we generated a total of 2,065,344 raw reads, of which 945,386 reads (45.77%) passed the quality and size filter. The highest proportion of them was assigned to Eukarya, followed by Bacteria and only a small proportion to Virus. However, we identified 24 viral families using distinct taxonomic assignment tools, including phages and vertebrate viruses, such as retroviruses and papillomaviruses. Also, we identified four bacterial genus already associated with disease in sloths. Our study sheds light on the microbiome of a previously unexplored species, further contributing to the comprehension of metagenomic global diversity.

Abstract In 2021, rubber bales appeared along the Brazilian coastline, causing environmental impacts. In this research paper, we identified a Second World War shipwreck that was associated with this event. Numerical simulations provided robust evidence to support the hypothesis that the origin of the 2021 bales was the MV Weserland . Moreover, it was sunk to a depth of approximately 5,000 m with a cargo containing rubber, tin, and wolframite. Tin is currently considered a strategic metal, with prices rising from USD 13,375/t in March 2020 to USD 34,462/t in May 2021. We provided evidence that the departure of rubber bales from the wreck and the increase in the price of its metal cargo were temporally associated; this reinforced the hypothesis of deep-sea unauthorized salvage in international waters (areas beyond national jurisdiction). The novel results demonstrate that historical shipwrecks are ticking time-bombs with risks of high and unmapped pollution in oceans worldwide.

Abstract This study evaluated the mechanical properties and the chemical and thermal characteristics of low-density polyethylene (LDPE) nanocomposites reinforced with graphene oxide for manufacturing via 3D printing. LDPE nanocomposites with different graphene oxide (GO) loadings (0.5% and 1.5%) were processed by a single-screw extruder. To characterize the pre-injected material, Fourier transform infrared spectroscopy (FTIR) and thermal characterization with thermogravimetry (TGA) were used. The injected material was also characterized with mechanical impact and traction tests. The results showed that incorporating GO into the polymeric matrix added hydroxyl groups to the matrix structure, with a 39.13% increase in Young's modulus for LDPE-1.5%wtGO samples. Mechanical tests of 3D printed samples showed that, with an increase in the amount of GO in the polymer matrix, the composite loses ductility, enabling its use in 3D printing technologies and thus encouraging new studies on printed materials for various applications.

Abstract Coastal zones are attractive for the development of economic interests (real estate and tourism) and are intensely occupied in a disorderly manner. Development in areas with available sediments can lead to conflicts between the natural movement of dunes and cities and communities and may result in burial processes. In addition, natural structures, such as dunes, play key roles in protecting against coastal erosion by balancing the beach-dune system with the sediment input, thus slowing the erosion process. This study aims to determine suitable areas for creating dune field dump sites in Barroquinha, Ceará, northeastern Brazil. The results showed that along the study area, the places with the greatest viability for creating dump sites were those furthest from the urban and permanent protection areas. In the stretch connecting Barroquinha City to the Bitupitá community on the CE-187 highway, 45 deposition points were monitored across five sectors under different environmental conditions. Sectors 2, 3, and 4 showed good responses to sediment fixation, in agreement with what was presented in the locational feasibility model. These results add to the discussion on urban infrastructure burying processes, making important contributions to urban planning and management in coastal areas. The results of this study will form an integral part of the Regional Contingency Plan for Incidents and/or situations of risk of environmental impacts in the coastal and/or oceanic zones of Ceará, which was conceived by the Chief Environment Scientist Program of Ceará State and contributes to the Action Plan against burying infrastructure by dune fields.

Abstract Background Congenital heart diseases (CHD) are one of the most prevalent malformations, and the screening tests to identify critical congenital heart disease (CCHD) is the pulse oximetry test, with subsequent investigation and treatment. Objective To quantify positive pulse oximetry tests and verify the prevalence of CCHD detected by it in asymptomatic newborns, ≥35 gestational weeks, in a Brazilian maternity hospital. Methodology This is an observational, retrospective, quantitative, analytical and cross-sectional study, conducted from October 2020 to May 2022, in a maternity hospital in southern Brazil, through the collection of records of positive oximetry pulse tests, following the norms of screening CCHD test of the Brazilian Society of Pediatrics (SBP), after they were evaluated with echocardiography for confirmation or exclusion of CHD. Results A total of 5,667 newborns were evaluated in this study, according to the inclusion criteria; 0.17% (n = 10) had a positive pulse oximetry test. Regarding the results of the echocardiography of the neonates with a positive test, two were normal, seven were cases of patent foramen ovale (PFO), and one was a case (0,017%) with interatrial communication (IAC) diagnosis. In the same period, five neonates with CCHD were born, symptomatic, diagnosed by physical examination, and referred to neonatal intensive care units (NICU) before taking the neonatal cardiac screening (< 24 hours of life). Conclusion The prevalence of positive pulse oximetry tests was 0.17% and none CCHD was detected. Five cases of CCHD were born in this period, but they were diagnosed before the recommend time to perform the screening test.

RESUMO Introdução: A rápida propagação do SARS-CoV-2 e as altas taxas de mutação durante a replicação viral levam à diversificação do vírus e ao surgimento de novas variantes. A vigilância genômica tem sido fundamental para monitorar as variantes do SARS-CoV-2 em todo o mundo. A supressão imunológica, conforme observada em pacientes com câncer, é um fator de risco para infecção por SARS-CoV-2 e covid-19 grave. Objetivo: Relatar uma vigilância genômica de dois anos do SARS-CoV-2 em pacientes com câncer acompanhados no Instituto Nacional de Câncer, Rio de Janeiro, Brasil. Método: Estudo observacional prospectivo. Foi avaliado um total de 384 amostras de swabs SARS-CoV-2+ coletadas entre outubro de 2020 e setembro de 2022. O pico do SARS-CoV-2 foi analisado por PCR e sequenciamento Sanger para determinar a variante infecciosa. Resultados: A maioria dos pacientes apresentava malignidades de órgãos sólidos (298/384; 77,6%) e 16,1% (62/384), doença metastática. Os casos graves de covid-19 representaram 29,4% (113/384) e foram registrados 27,1% (104/384) óbitos. Em relação às variantes infectantes do SARS-CoV-2, as mais comuns foram Gamma (n=137) e Ômicron (BA.1) (n=73). A distribuição da variante ao longo do tempo foi semelhante ao que foi relatado para a população geral do Brasil no mesmo período. Quando analisadas as topografias de câncer dos pacientes, a Gamma infectou pacientes com câncer de mama (47/137; 34,3%) e cervical (11/137; 8%), mais frequentemente do que outras variantes, enquanto a Ômicron predominou entre reto (10/122; 8,2%) e câncer de próstata (8/122; 6,6%) em comparação com outras variantes. Conclusão: A vigilância genômica é uma ferramenta importante para identificar e avaliar o impacto das variantes do SARS-CoV-2, e deve prosseguir especialmente em populações imunossuprimidas.

ABSTRACT Introduction: The fast SARS-CoV-2 spread and high mutation rates during viral replication led to virus diversification and the emergence of new variants. Genomic surveillance has been key to monitoring SARS-CoV-2 variants across the globe. Immune suppression, as observed in cancer patients, is a risk factor for SARS-CoV-2 infection and severe COVID-19. Objective: To report a two-year genomic surveillance of SARS-CoV-2 in cancer patients followed up at the Brazilian National Cancer Institute, Rio de Janeiro, Brazil. Method: Prospective observational study with 384 SARS-CoV-2+ swabs specimens collected and evaluated between October 2020 and September 2022. SARS-CoV-2 spike was analyzed by PCR and Sanger sequencing to determine the infecting variant. Results: Most of the patients had solid organ malignancies (298/384; 77.6%) and 16.1% (62/384) had metastatic disease. Severe COVID-19 cases accounted for 29.4% (113/384) and 27.1% (104/384) of deaths registered. The most common SARS-CoV-2 infecting variants were Gamma (n=137) and Omicron (BA.1) (n=73). The variant distribution overtime was similar to what has been reported for the general population of Brazil in the same period. When patients’ cancer topographies were analyzed, it was found that Gamma infected patients with breast (47/137; 34.3%) and cervical (11/137; 8%) cancer were more frequent than other variants, while Omicron predominated among rectum (10/122; 8.2%) and prostate (8/122; 6.6%) cancer compared to other variants. Conclusion: Genomic surveillance is an important tool for identifying and evaluating the impact of SARS-CoV-2 variants, and should continue especially in immunosuppressed populations.

RESUMEN Introducción: La rápida propagación del SARS-CoV-2 y las altas tasas de mutación durante la replicación viral conducen a la diversificación del virus y la aparición de nuevas variantes. La vigilancia genómica ha sido clave para monitorear las variantes del SARS-CoV-2 en todo el mundo. La inmunosupresión, como se observa en pacientes con cáncer, es un factor de riesgo de infección por SARS-CoV-2 y COVID-19 grave. Objetivo: Informar una vigilancia genómica de dos años del SARS-CoV-2 en pacientes con cáncer seguida en el Instituto Nacional de Cáncer, Río de Janeiro, Brasil. Método: Estudio observacional prospectivo. Se evaluaron un total de 384 muestras de hisopos de SARS-CoV-2+ recolectadas entre octubre de 2020 y septiembre de 2022. El pico de SARS-CoV-2 se analizó mediante PCR y secuenciación Sanger para determinar la variante infectante. Resultados: La mayoría de los pacientes tenían neoplasias malignas de órganos sólidos (298/384; 77,6%) y el 16,1% (62/384), enfermedad metastásica. Los casos graves de COVID-19 representaron el 29,4% (113/384) y se registraron el 27,1% (104/384) de las muertes. En cuanto a las variantes infectantes del SARS-CoV-2, las más comunes fueron Gamma (n=137) y Ómicron (BA.1) (n=73). La distribución de variantes en el tiempo fue similar a lo reportado para la población general del Brasil en el mismo período. Cuando se analizaron las topografías de cáncer de los pacientes, la Gamma infectó a los pacientes con cáncer de mama (47/137; 34,3%) y de cuello uterino (11/137; 8%) con mayor frecuencia que otras variantes, mientras que Ómicron predominó entre el recto (10/122; 8,2%) y cáncer de próstata (8/122; 6,6%) en comparación con otras variantes. Conclusión: La vigilancia genómica es una herramienta importante para identificar y evaluar el impacto de las variantes del SARS-CoV-2 y debe continuarse, especialmente en poblaciones inmunodeprimidas.

Abstract Background Coronavirus disease 2019 (COVID-19) has emerged as a public health emergency worldwide, predominantly affecting the respiratory tract. However, evidence supports the involvement of extrapulmonary sites, including reports of intracranial hemorrhages. Objective To describe six original cases and review the literature on intracranial hemorrhages in patients diagnosed with COVID-19 by molecular methods. Methods A systematic literature review was performed on MEDLINE, PubMed, and NCBI electronic databases to identify eligible studies. Of the total 1,624 articles retrieved, only 53 articles met the inclusion criteria. Results The overall incidence of intracranial hemorrhage in patients hospitalized for COVID-19 was 0.26%. In this patient group, the mean age was 60 years, and the majority were male (68%) with initial respiratory symptoms (73%) and some comorbidity. Before the diagnosis of hemorrhage, 43% of patients were using anticoagulants, 47.3% at therapeutic doses. The intraparenchymal (50%) was the most affected compartment, followed by the subarachnoid (34%), intraventricular (11%), and subdural (7%). There was a predominance of lobar over non-lobar topographies. Multifocal or multicompartmental hemorrhages were described in 25% of cases. Overall mortality in the cohort studies was 44%, while around 55% of patients were discharged from hospital. Conclusion Despite the unusual association, the combination of these two diseases is associated with high rates of mortality and morbidity, as well as more severe clinicoradiological presentations. Further studies are needed to provide robust evidence on the exact pathophysiology behind the occurrence of intracranial hemorrhages after COVID-19 infection.

Resumo Antecedentes A COVID-19 emergiu como uma emergência de saúde pública em todo o mundo, proporcionando lesão principalmente do trato respiratório. No entanto, várias evidências apontam para acometimento de sítios extrapulmonares, incluindo relatos de hemorragias intracranianas. Objetivo Descrever seis casos originais e revisar a literatura sobre hemorragias intracranianas em pacientes com diagnostico de COVID-19 por métodos moleculares. Métodos A revisão sistemática da literatura foi feita nas bases de dados eletrônicas da MEDLINE, PubMed e NCBI para identificar os estudos elegíveis. Do total de 1.624 artigos recuperados, apenas 53 artigos preencheram os critérios de inclusão. Resultados A incidência geral de hemorragia intracraniana nos pacientes internados por COVID-19 foi de 0,26%. A média de idade foi de 60 anos, e a maioria dos pacientes era do sexo masculino (68%) com sintomas respiratórios iniciais (73%) e alguma comorbidade. Antes do diagnóstico de hemorragia, 43% estavam em uso de anticoagulantes, 47,3% destes em doses terapêuticas. O compartimento mais acometido foi o intraparenquimatoso (50%), seguido do subaracnoideo (34%), intraventricular (11%) e subdural (7%). Houve predomínio de topografias lobares sobre as não-lobares. Hemorragias multifocais ou multicompartimentais foram descritas em 25% dos casos. A mortalidade geral nos estudos de coorte foi de 44%, enquanto houve alta hospitalar em cerca de 55% dos pacientes. Conclusão Apesar da associação incomum, a combinação dessas doenças está relacionada com altas taxas de mortalidade e morbidade, bem como apresentações clínico-radiológicas mais graves. Mais estudos são necessários para oferecer evidências robustas sobre a fisiopatologia exata por trás da ocorrência de hemorragias intracranianas após infecção por COVID-19.

Abstract We evaluate how the concentrations of inorganic nutrients and chlorophyll a vary in a heterogeneous area (Equatorial SW Atlantic), covering a gradient from stations closer to the coast to others more distant associated or not with turbid-zone reefs. Vertical temperature (27.9 ± 0.10 °C; mean ± standard deviation) and salinity (36.2 ± 0.14) profles showed that the water column is well mixed (0-30 m depth). The oligotrophic condition was marked by low concentrations of phosphate (0.30 ± 0.22 µM) and dissolved inorganic nitrogen (0.64 ± 0.74 µM). Moreover, dissolved reactive silicon (DSi) was low in most samples (< 2.0 µM), but higher (>10 µM) in nearshore stations, probably related to continental runoff and/or resuspension of the bottom sediments. The pelagic phytoplankton biomass indicated that chlorophyll a (0.25 ± 0.08 µg L-1) was low, positively correlated with light and negatively correlated with nutrients, indicating possible phytoplankton uptake. Chlorophyll a concentrations were lower in stations closer to the coast and higher in some stations near the reefs, indicating that the latter could be more prone to phytoplankton development and depletion of nutrients, especially DSi. Therefore, although oligotrophy is present along this coast, we found some unexpected heterogeneity of nutrient and chlorophyll a distributions, which were probably infuenced by benthic-pelagic coupling due to the presence of extensive reefs, sponge gardens (18-30 m depth), and the proximity to the coast. These results highlight the importance of understanding the heterogeneity of ocean productivity, especially in lesser known low-latitude areas, which showed distinct nutrient and chlorophyll a levels related to the occurrence of tropical reefs that are capable of supporting important fsh stocks and unique biological communities. Equatorial Atlantic, Atlantic , Atlantic) turbidzone turbid zone 27.9 279 27 9 (27. 010 0 10 0.1 °C C deviation 36.2 362 36 2 (36. 0.14 014 14 030 30 (0-3 depth. depth . depth) 0.30 (0.3 022 22 0.2 µM 0.64 064 64 (0.6 074 74 0.7 µM. Moreover DSi (DSi < ( 20 2. µM, >10 (>1 andor sediments 0.25 025 25 (0.2 008 08 0.0 L1 L 1 L-1 uptake Therefore distributions benthicpelagic benthic 1830 18 (18-3 depth, productivity lowlatitude latitude areas communities 27. (27 01 0. 36. 3 (36 03 (0- 0.3 (0. 02 0.6 06 6 07 7 >1 (> 00 L- 183 (18- (2 (3 (0 > (18 (1

Abstract Marginal reefs can provide meaningful information about the structure and dynamics of ecosystems under suboptimal environmental conditions. In addition to their different characteristics, these environments can also occur in urbanized areas. In this note, we characterize marine animal forests (MAFs) on turbid-zone reefs on an urban coast in the equatorial southwestern Atlantic. Overall, the sandstone ferruginous reefs (6-10 m depth) exhibited a flat topography and gentle slope (1-2 m above the seabed). Benthic cover is composed mainly of sponge gardens and ascidians. In addition, we found a low-relief coral carpet with only one massive reef-building coral (Siderastrea stellata) and Zoantharia. The ascidians and sponges had a higher diversity (at least 15 species) than the cnidarians (two species) in these forests. The main animals forming this seascape are weedy and stress-tolerant species adapted to challenging environmental conditions, such as swell waves, mesotidal regimes, moderate turbidity waters, and periodic burial. In this regard, these conditions and human impacts have shaped a unique MAF. Remarkably, the studied formations seem similar to high-latitude marginal reefs or low-latitude reefs under the influence of upwelling, which sustains soft corals and non-framework building coral communities along with sponges and ascidians. In particular, the shallow MAFs along the semi-arid coast of Brazil seem to lack some of the characteristics of low-latitude reefs under high sedimentation, whose structure was described as coral rubble within sedimentary matrices. This suggests that factors other than periodic burials and low light availability affected these MAFs. These overlooked forests are widespread in this area and have been neglected in studies, despite their richness (> 31 taxa) and valuable ecosystem goods and services. In the context of urbanized areas subject to climate change and pollution impacts, jetties, and dredging activities, it is necessary to consider these lush forests in impact assessments and conservation policies. note (MAFs turbidzone turbid zone Atlantic Overall 610 6 10 (6-1 depth 12 1 2 (1- seabed. seabed . seabed) lowrelief relief reefbuilding reef Siderastrea stellata Zoantharia at two stresstolerant stress tolerant waves regimes waters burial regard MAF Remarkably highlatitude latitude lowlatitude upwelling nonframework non framework particular semiarid semi arid sedimentation matrices studies > ( 3 taxa services jetties activities policies 61 (6- (1 (6

Abstract A complete genome of the first anellovirus infecting the wild felid Leopardus pardalis (ocelot) and a partial genome were assembled and annotated through high-throughput sequencing protocols followed by Sanger sequencing validation. The full-length virus obtained comprises 2,003 bp, while the partial genome comprises 1,224 bp. Phylogenetic analysis grouped these two sequences in two distinct clusters related to previously described Felidae anelloviruses. The ORF1 of the partial genome was identified as a new species provisionally called Torque teno ocelot virus, with 53.6% identity with its sister lineage. The complete genome was inferred as a new representative of the Torque teno felid virus 3 species, with 73.28% identity to the closest reference. This study expands known virus diversity and the host span of anelloviruses.

Abstract This study identified the marine protected areas (MPAs) affected by the most extensive oil spill recorded on the Southwestern Atlantic coast, Brazil (2019/2020). We found that 81 MPAs suffered the direct or indirect effects of spilled oil, producing chemical, biological and socioenvironmental impacts over approximately 3.0% of the 2,659 protected areas currently established in Brazil. Although estimates suggest a moderate volume of spilled oil, the incident reached wide coastal strips, probably producing more damage to MPAs than other cases worldwide. Further, the generated negative impacts affected the already fragile environmental protection system in Brazil, potentially leading to negative impacts on global networks of MPAs and worldwide biodiversity.

Resumen Esta investigación tuvo como objetivo analizar la existencia de relaciones positivas, negativas, directas e indirectas (a través de la satisfacción laboral y la autoeficacia) entre la percepción de una vocación profesional y la intención de dejar el empleo, así como el papel moderador de la percepción de la movilidad laboral. Se utilizó un enfoque cuantitativo, descriptivo, con corte transversal y recopilación de datos primarios de 230 empleados. Para analizar los datos se adoptó un modelo de ecuaciones estructurales. Los resultados muestran que existe un efecto directo y negativo entre la percepción de una vocación y la intención de dejar el empleo, así como entre la satisfacción laboral y la intención de dejar el empleo. Se identificó el efecto directo y positivo entre percibir una vocación y la satisfacción laboral y entre percibir una vocación y autoeficacia. La satisfacción laboral media parcialmente la relación entre percibir una vocación y la intención de dejar el empleo. No existe un efecto significativo entre la autoeficacia y la intención de dejar el empleo en la muestra analizada. El efecto moderador de la movilidad laboral sobre la relación entre la satisfacción laboral y la autoeficacia con la intención de dejar el empleo no fue significativo. positivas negativas a cuantitativo descriptivo 23 empleados estructurales analizada 2

Abstract This research aimed to analyze the existence of positive, negative, direct, and indirect relationships (via job satisfaction and self-efficacy) between perceiving a calling and turnover intention, as well as the moderating role of perceived mobility. We used a quantitative approach and data collection from 230 employees. The analysis was conducted using structural equation modeling. The results show a direct and negative effect between perceiving a calling and turnover intention, and between job satisfaction and turnover intention. We identified a direct and positive effect between perceiving a calling and job satisfaction and between perceiving a calling and self-efficacy. Job satisfaction partially mediates the relationship between perceiving a calling and turnover intention. There is no significant effect between self-efficacy and turnover intention in the analyzed sample. The moderating effect of perceived mobility on the relationship between job satisfaction and self-efficacy with turnover intention was not significant. via selfefficacy self efficacy 23 employees modeling selfefficacy. efficacy. sample 2

Resumo Esta pesquisa teve como objetivo analisar a existência de relações positivas, negativas, diretas e indiretas (via satisfação no trabalho e autoeficácia) entre perceber um chamado e a intenção de deixar um emprego, assim como o papel moderador da percepção de mobilidade de emprego. Utilizou-se uma abordagem quantitativa, descritiva, com corte transversal e coleta de dados primários junto a 230 funcionários. Para analisar os dados, adotou-se a modelagem de equações estruturais. Os resultados apontam que há um efeito direto e negativo entre perceber um chamado e intenção de deixar o emprego, bem como entre satisfação no trabalho e a intenção de deixar o emprego. Identificou-se o efeito direto e positivo entre perceber um chamado e satisfação no trabalho e entre perceber um chamado e a autoeficácia. A satisfação no trabalho medeia parcialmente a relação entre perceber um chamado e a intenção de deixar o emprego. Não há efeito significativo entre autoeficácia e a intenção de deixar o emprego na amostra analisada. O efeito moderador da mobilidade de emprego nas relações entre satisfação do trabalho e autoeficácia com intenção de deixar o emprego não foram significativas. positivas negativas via Utilizouse Utilizou se quantitativa descritiva 23 funcionários adotouse adotou estruturais Identificouse Identificou analisada significativas 2

Resumo O objetivo foi analisar a tendência, a espacialização e as circunstâncias associadas às violências contra populações vulneráveis no Brasil, entre 2009 e 2017. Lançou-se mão de análise descritiva, de espacialização e de regressão polinomial. Notificaram-se 1.116.331 casos de violência, com aumento de 667,5% no período analisado. Em todas as populações estudadas, houve predomínio da violência física por meio de força corporal contra mulheres de baixa escolaridade, praticada por homens em residências. As violências psicológica e sexual destacaram-se nas populações feminina e LGBT. Já a negligência foi expressiva dentre pessoas com deficiência, crianças/adolescentes e idosas. Pelo menos 65% das notificações de violência no Brasil foram caracterizadas como indiscriminadas quanto à motivação do ato. A análise de tendência demonstrou a ascensão da prevalência de violência contra mulheres (+8,5%), pessoas com deficiência (+7,1%), idosos (+55,4%), indígenas (+99,4%), LGBT (+3,4%) e pretos, pardos e amarelos (+30,2%), exceto para a população de crianças/adolescentes (-11,4%). No Norte/Nordeste prevaleceu agressão contra mulheres e crianças/adolescentes, ao passo que no Centro-Oeste, Sul e Sudeste, as populações LGBT, deficiente e idosa foram mais vilipendiadas.

Abstract The scope of this study was to analyze the trend, spatialization and circumstances associated with violence against vulnerable populations in Brazil between 2009 and 2017. Descriptive analysis, spatialization and polynomial regression were used. A total of 1,116,331 cases of violence were notified, with an increase of 667.5% over the period. In all populations under research there was a predominance of physical violence against women with low levels of schooling, perpetrated by men in residences. Sexual and psychological violence was highlighted in female and LGBT populations. Negligence, however, was significant among people with disabilities, children/adolescents, and the elderly. At least 65% of the notifications of violence in Brazil were characterized as indiscriminate regarding the motivation behind the act. Tendency analysis showed a rise of interpersonal violence against women (+8.5%), disabled people (+7.1%), elderly individuals (+55.4%), natives (+99.4%), LGBT people (+3.4%) and racial violence (+30.2%), except for violence against underaged individuals, which showed a decrease (-11.4%). In North and Northwest regions violence against women and underaged individuals prevailed, while in Midwest, South and Southeast regions the LGBT, disabled and elderly populations were most subject to abuse.

Many addresses where Machado de Assis lived are known to his biographers, but it seems likely that others are still unknown. From the discovery of a record in a newspaper at the time, this research seeks to ascertain whether the writer would have inhabited Rua da Alfândega 123 in 1867. In the absence of other direct evidence, parallel information is sought that could disprove the data, without result that disappointed him. In addition, an investigation is carried out on the property, in order to understand its plan and check if it still exists in Rio de Janeiro today. Finally, this space is related to a passage from Machado's narrative work.

Sus biógrafos conocen muchas direcciones donde vivió Machado de Assis, pero parece probable que aún se desconozcan otras. A partir del descubrimiento de un registro en un periódico de la época, esta investigación busca determinar si el escritor habría vivido en 1867 en la Rua da Alfândega 123. En ausencia de otra evidencia directa, se busca información paralela que pueda refutar los datos. el que no encontró ningún resultado que lo decepcionaría. Además, se lleva a cabo una investigación en la propiedad con el fin de entender su plano y verificar si el edificio aún existe en Río de Janeiro en la actualidad. Finalmente, la calle se relaciona con un pasaje de la obra narrativa de Machado.

Muitos endereços em que viveu Machado de Assis são conhecidos por seus biógrafos, mas parece provável que outros estariam ainda incógnitos. A partir da descoberta de um registro em jornal da época, essa pesquisa busca averiguar se o escritor teria habitado em 1867 na rua da Alfândega 123. Na falta de outras evidências diretas, buscam-se informações paralelas que possam vir a desmentir o dado, o que não encontrou resultado que o desabonasse. Além disso, procede-se a uma investigação sobre o imóvel, a fim de compreender sua planta e verificar se o prédio ainda existe no Rio de Janeiro atual. Por fim, o logradouro é relacionado com uma passagem da obra narrativa machadiana.