The herbaceous shrub Tetradenia riparia has been traditionally used to treat inflammatory and infectious diseases. Recently, a study showed that T. riparia essential oil (TrEO) obtained in summer has antileishmanial effects, although these results could be influenced by seasonal variation. This study evaluated the activity of the TrEO obtained in different seasons against Leishmania (Leishmania) amazonensis, in vitro and in vivo. The compounds in the TrEO were analysed by gas chromatography-mass spectrometry; terpenoids were present and oxygenated sesquiterpenes were the majority compounds (55.28%). The cytotoxicity and nitric oxide (NO) production were also tested after TrEO treatment. The TrEO from all seasons showed a 50% growth inhibitory concentration for promastigotes of about 15 ng/mL; at 30 ng/mL and 3 ng/mL, the TrEO reduced intracellular amastigote infection, independently of season. The TrEO from plants harvested in summer had the highest 50% cytotoxic concentration, 1,476 ng/mL for J774.A1 macrophages, and in spring (90.94 ng/mL) for murine macrophages. NO production did not change in samples of the TrEO from different seasons. The antileishmanial effect in vivo consisted of a reduction of the parasite load in the spleen. These results suggest that the TrEO has potential effects on L. (L.) amazonensis, consonant with its traditional use to treat parasitic diseases.

OBJECTIVE: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous leishmaniasis (ACL) diagnosis. MATERIAL AND METHODS: Five pairs of primers, four of them derived from Leishmania kDNA sequences (MP3H-MP1L; B1-B2; LBF1-LBR1; 13A-13B), and one derived from the SL RNA (mini-exon) gene repeat (LU5A-LB3C), reported previously, were used. RESULTS: The MP3H-MP1L primers were the best at amplifying the DNA, detecting 2 fg of Leishmania spp. DNA. The 13A-13B primers presented the worst performance, detecting 512 x 10³ fg of DNA. CONCLUSION: The wide variation in the analytical sensitivity of the primers used in the PCR, and the significant differences from the conventional method of ACL diagnosis found in this study, emphasize the importance of standardizing the PCR technique, analyzing sensitivity, and selecting suitable oligonucleotide primers.

The aim of this work was to evaluate the effect of broiler breeders' age and yeast wall (YW) levels on broilers' performance and macrophage activitys. The experiment consisted in a completely randomized design and a 2 X 5 factorial arrangement and two controls of two broiler breeders age (34 and 57 weeks of age) and five YW levels (zero, one, two, three or four kg of YW/ton of diet). They received sorghum diet compared to a control corn/soybean meal diet. The age of the broiler breeders influenced the performance, but did not affect macrophage activity. At 34 weeks age broiler breeders' progeny, all diets were similar considering the average weight. For 57 weeks age broiler breeders' progeny, at 21 days, only average weight of sorghum diet supplemented with 4 kg/ton was similar to corn diet results. The optimum level of YW for maximal macrophage activity was 2.06 kg/ton of meal.

São escassas as informações sobre o papel da mobilidade populacional na manutenção da leishmaniose tegumentar americana no estado do Paraná. Avalia-se a mobilidade populacional como fator de risco para esta endemia em três mesorregiões do Paraná, utilizando dados gerados na Universidade Estadual de Maringá, no período de 1987 a 2004. Foram notificados 1.933 casos, predominando os casos migrantes (54,4%). Os municípios com maior número de casos notificados foram Maringá (358), Doutor Camargo (108) e Terra Boa (105). Os casos rurais foram predominantemente autóctones (89,8%), enquanto os urbanos, na maioria (84,8%) migrantes (p<0,0001). Para os casos rurais autóctones, não houve predomínio entre os sexos (p=0,127); para os casos urbanos migrantes, prevaleceu o sexo masculino (p<0,0001). Os casos migrantes foram na maioria relacionados com a mobilidade intra e intermunicipal. A mobilidade populacional parece ser uma variável importante na epidemiologia desta doença no Estado do Paraná.

Information on the role of population mobility in maintaining American tegumentary leishmaniasis in the State of Paraná is scarce. Population mobility was evaluated as a risk factor for this endemic disease in three mesoregions of Paraná, using data built up at the State University of Maringá, covering 1987 to 2004. A total of 1,933 cases were notified, mostly among migrants (54.4%). The municipalities with the greatest numbers of cases notified were Maringá (358), Doutor Camargo (108) and Terra Boa (105). The rural cases were predominantly autochthonous (89.8%), while the urban cases were mostly among migrants (84.8%) (p < 0.0001). Among the rural autochthonous cases, there was no difference between the sexes (p = 0.127), whereas among the urban migrant cases, men predominated (p < 0.0001). The migrant cases were mostly related to mobility within and between municipalities. Population mobility seems to be an important variable in the epidemiology of this disease in the State of Paraná.

Avalia-se o atendimento ambulatorial oferecido aos pacientes com leishmaniose tegumentar americana, de 2001 a 2004, antes e após a descentralização, em municípios do noroeste do Estado do Paraná, Brasil. Foram analisados 229 prontuários de pacientes com leishmaniose tegumentar americana. Observou-se que no atendimento centralizado a avaliação clínica e os registros médicos foram mais completos e criteriosos; o tratamento iniciou-se mais prontamente; o acompanhamento do desfecho em 95% dos pacientes e em 100% dos casos tratados com duas séries de tratamento foi avaliado. Foram observados os critérios recomendados pelo Manual de Controle da Leishmaniose Tegumentar Americana. Após a descentralização, o rigor no atendimento aos pacientes diminuiu: 32% dos pacientes foram tratados com duas séries de antimoniato N-metil glucamina, sem observação dos critérios recomendados. Detectou-se que 72,6% do acompanhamento dos pacientes foi inadequado e 84% da avaliação clínica não foram realizadas. A falta de registro dos prontuários constituiu importante obstáculo para a avaliação das atividades. O presente estudo não evidenciou benefícios significativos com a descentralização.

The current study focuses on outpatient treatment of patients (n = 229) with American cutaneous leishmaniasis from 2001 to 2004, before and after decentralization of treatment to the municipal level in northwestern Paraná State, Brazil. While the treatment was still centralized, clinical evaluation and medical records were more complete and detailed, treatment was initiated earlier, and follow-up of the outcome was evaluated in 95% of patients and 100% of cases that received two treatment cycles. Treatment also complied with the guidelines of the American Cutaneous Leishmaniasis Control Manual. After decentralization, treatment was less rigorous: 32% of patients were treated with two cycles of N-methyl glucamine antimoniate without complying with the guidelines; 72.6% of patients received inadequate treatment; 84% of cases failed to received proper clinical follow-up. Lack of information on patient clinical records posed a serious obstacle to evaluation. The current study failed to identify any benefit in decentralizing treatment of American cutaneous leishmaniasis to the local level.

Avalia-se o atendimento ambulatorial oferecido aos pacientes com leishmaniose tegumentar americana, de 2001 a 2004, antes e após a descentralização, em municípios do noroeste do Estado do Paraná, Brasil. Foram analisados 229 prontuários de pacientes com leishmaniose tegumentar americana. Observou-se que no atendimento centralizado a avaliação clínica e os registros médicos foram mais completos e criteriosos; o tratamento iniciou-se mais prontamente; o acompanhamento do desfecho em 95% dos pacientes e em 100% dos casos tratados com duas séries de tratamento foi avaliado. Foram observados os critérios recomendados pelo Manual de Controle da Leishmaniose Tegumentar Americana. Após a descentralização, o rigor no atendimento aos pacientes diminuiu: 32% dos pacientes foram tratados com duas séries de antimoniato N-metil glucamina, sem observação dos critérios recomendados. Detectou-se que 72,6% do acompanhamento dos pacientes foi inadequado e 84% da avaliação clínica não foram realizadas. A falta de registro dos prontuários constituiu importante obstáculo para a avaliação das atividades. O presente estudo não evidenciou benefícios significativos com a descentralização.

The current study focuses on outpatient treatment of patients (n = 229) with American cutaneous leishmaniasis from 2001 to 2004, before and after decentralization of treatment to the municipal level in northwestern Paraná State, Brazil. While the treatment was still centralized, clinical evaluation and medical records were more complete and detailed, treatment was initiated earlier, and follow-up of the outcome was evaluated in 95% of patients and 100% of cases that received two treatment cycles. Treatment also complied with the guidelines of the American Cutaneous Leishmaniasis Control Manual. After decentralization, treatment was less rigorous: 32% of patients were treated with two cycles of N-methyl glucamine antimoniate without complying with the guidelines; 72.6% of patients received inadequate treatment; 84% of cases failed to received proper clinical follow-up. Lack of information on patient clinical records posed a serious obstacle to evaluation. The current study failed to identify any benefit in decentralizing treatment of American cutaneous leishmaniasis to the local level.