RESUMEN Introducción: La medicina regenerativa y terapia celular representa una alternativa segura y eficaz en la regeneración hística. La fibrina rica en plaquetas y leucocitos favorece la cicatrización de la base craneal, con una disminución significativa en las complicaciones, en especial la fístula de líquido cefalorraquídeo. Objetivo: Describir los resultados del empleo de la fibrina rica en plaquetas y leucocitos como elemento accesorio en la reparación de la base craneal. Métodos: Se realizó un estudio descriptivo, transversal en 250 pacientes en el Hospital Hermanos Ameijeiras, operados por procedimientos endonasales endoscópicos con diversos tumores de la base craneal, en los cuales se empleó la fibrina rica en plaquetas y leucocitos durante la fase de reconstrucción. Se realizó una evaluación de la barra de reparación y las complicaciones presentes. Para el análisis de los datos se utilizaron frecuencias absolutas y relativas como medidas resumen. Resultados: El 97,2 % de las barreras de reparación fue catalogada de óptima. Se reporta con el uso de la fibrina rica en plaquetas y leucocitos 2,0 % de fístula de líquido cefalorraquídeo, 0,8 % de infección del sistema nervioso central, 4,0 % de costras nasales posoperatorias. Conclusiones: El presente estudio evidencia el efecto positivo del empleo de la fibrina rica en plaquetas y leucocitos en la reparación del base craneal con gran impacto en el índice de fístula de líquido cefalorraquídeo y la calidad de vida nasosinusal.

ABSTRACT Introduction: Regenerative medicine and cell therapy represents a safe and effective alternative in tissue regeneration. Fibrin rich in platelets and leukocytes promotes healing of the cranial base, with a significant decrease in complications, especially cerebrospinal fluid leak. Objective: Describe the results of using fibrin rich in platelets and leukocytes as an accessory element in the repair of the cranial base. Methods: A descriptive, cross-sectional study was carried out in 250 patients at the “Hermanos Ameijeiras” Hospital operated by endoscopic endonasal procedures with various tumors of the cranial base, in which fibrin rich in platelets and leukocytes was used during the reconstruction phase. An evaluation of the repair bar and the complications present was performed. For data analysis, absolute and relative frequencies were used as summary measures. Results: 97.2% of the repair barriers were classified as optimal. With the use of fibrin rich in platelets and leukocytes, 2.0% of cerebrospinal fluid leak, 0.8% of central nervous system infection, 4.0% of postoperative nasal scabs are reported. Conclusions: The present study evidences the positive effect of the use of leukocyte-platelet-rich fibrin in the repair of the skull cranial base, with great impact on the rate of cerebrospinal fluid leak and sinonasal quality of life.

Abstract Background: The bioactive peptides derived from snake venoms of the Viperidae family species have been promising as therapeutic candidates for neuroprotection due to their ability to prevent neuronal cell loss, injury, and death. Therefore, this study aimed to evaluate the cytoprotective effects of a synthetic proline-rich oligopeptide 7a (PRO-7a; <EDGPIPP) from Bothrops jararaca snake, on oxidative stress-induced toxicity in neuronal PC12 cells and astrocyte-like C6 cells. Methods: Both cells were pre-treated for four hours with different concentrations of PRO-7a, submitted to H2O2-induced damage for 20 h, and then the oxidative stress markers were analyzed. Also, two independent neuroprotective mechanisms were investigated: a) L-arginine metabolite generation via argininosuccinate synthetase (AsS) activity regulation to produce agmatine or polyamines with neuroprotective properties; b) M1 mAChR receptor subtype activation pathway to reduce oxidative stress and neuron injury. Results: PRO-7a was not cytoprotective in C6 cells, but potentiated the H2O2-induced damage to cell integrity at a concentration lower than 0.38 μM. However, PRO-7a at 1.56 µM, on the other hand, modified H2O2-induced toxicity in PC12 cells by restoring cell integrity, mitochondrial metabolism, ROS generation, and arginase indirect activity. The α-Methyl-DL-aspartic acid (MDLA) and L-NΩ-Nitroarginine methyl ester (L-Name), specific inhibitors of AsS and nitric oxide synthase (NOS), which catalyzes the synthesis of polyamines and NO from L-arginine, did not suppress PRO-7a-mediated cytoprotection against oxidative stress. It suggested that its mechanism is independent of the production of L-arginine metabolites with neuroprotective properties by increased AsS activity. On the other hand, the neuroprotective effect of PRO-7a was blocked in the presence of dicyclomine hydrochloride (DCH), an M1 mAChR antagonist. Conclusions: For the first time, this work provides evidence that PRO-7a-induced neuroprotection seems to be mediated through M1 mAChR activation in PC12 cells, which reduces oxidative stress independently of AsS activity and L-arginine bioavailability. Background loss injury death Therefore prolinerich proline rich PRO7a PROa PRO (PRO-7a <EDGPIPP EDGPIPP stressinduced induced PC PC1 astrocytelike astrocyte like C Methods pretreated pre treated PRO7a, 7a, H2O2induced HOinduced H2O2 H O 2 h analyzed Also investigated Larginine L arginine (AsS b M Results 038 0 38 0.3 μM However 156 1 56 1.5 µM hand metabolism αMethylDLaspartic α Methyl DL aspartic MDLA (MDLA LNΩNitroarginine NΩ Nitroarginine LName, LName Name , (L-Name) NOS, NOS (NOS) Larginine, arginine, PRO7amediated PROamediated DCH, DCH (DCH) antagonist Conclusions time PRO7ainduced PROainduced bioavailability HO H2O 03 3 0. 15 5 1. (L-Name (NOS amediated (DCH ainduced

Resumo Discute-se neste artigo o papel desempenhado pela Gaceta Médica de México no processo de institucionalização e profissionalização da medicina científica no país entre 1860 e 1890. A partir da noção de tecnologia literária utilizada por Steven Shapin e Simon Schaffer e da análise qualitativa de relatórios médicos com enunciados típicos, examina-se o modo como os membros da Academia Nacional de Medicina, instituição responsável pelo periódico, e da Escola Nacional de Medicina coletivizaram suas experiências e prescreveram formas de ser e agir por meio de suas publicações. Busca-se demonstrar o caráter de “manual não sistematizado” da publicação, importante instrumento de normatização e regulação dos saberes médicos no México nesse período.

Abstract This article discusses the role played by the Gaceta Médica de México in the process of institutionalizing and professionalizing scientific medicine in Mexico from 1860 to 1890. From the notion of literary technology utilized by Steven Shapin and Simon Schaffer and qualitative analysis of medical reports containing typical discourse, we examine how members of the Mexican National Academy of Medicine (the institution responsible for this journal) and the National School of Medicine collected their experiences and prescribed ways of being and acting through their publications. The aim is to demonstrate how this periodical acted as an unsystematized manual, an important instrument for normalizing and regulating medical knowledge in Mexico during this period.

RESUMO A couve (Brassica oleracea var. acephala) é geralmente produzida por agricultores urbanos no Brasil, mas existem poucos estudos sobre a diversidade e manejo das doenças para produção sustentável em cultivos urbanos. Neste estudo, foi verificada a ocorrência de doenças na couve em cultivos urbanos de duas regiões do Brasil, representando as condições climáticas de Cwa (Lavras MG) e Aw (Campina Grande PB) pela classificação de Köppen Geiger e os fatores que contribuem para a ocorrência das enfermidades. Os produtores foram visitados semanalmente em cada região por oito meses e foi realizado o levantamento das doenças, bem como as principais táticas de manejo aplicadas pelos agricultores de cada município. Em ambas as regiões, o oídio (Erysiphe polygoni) e a podridão negra (Xanthomonas campestris pv. campestris) foram as doenças predominantes. A umidade relativa e alta temperatura foram os fatores ambientais que contribuíram para o aumento das doenças. O manejo com cobertura vegetal contribuiu para diminuir o oídio e para manter a podridão negra. Consequentemente, o cultivo intensivo de couve proporcionou um aumento da severidade da podridão negra. Esses resultados mostram a importância de recomendações adequadas de táticas de manejo utilizadas na agricultura urbana visando o controle de doenças da couve de acordo com as condições climáticas da área.

ABSTRACT Kale (Brassica oleracea var. acephala) is mostly produced by urban growers in Brazil, but little is known about the diversity and the management of diseases for sustainable urban agriculture. The present study investigated the occurrence of diseases in the urban cultivation of kale in two regions of Brazil, representing the climate conditions Cwa (Lavras - MG) and Aw (Campina Grande - PB) according to Köppen Geiger classification, as well as the factors that contribute to such occurrence. Growers in each region were visited every week during eight months while a survey was carried out on the diseases and the major crop management practices. In both regions, powdery mildew (Erysiphe polygoni) and black rot (Xanthomonas campestris pv. campestris) were the prevalent diseases. Relative humidity and high temperature were the environmental factors that contributed to increase the diseases. Management using straw mulch favored powdery mildew reduction and black rot maintenance. Consequently, kale intensive production resulted in higher black rot severity. These results demonstrated the importance of proper recommendations on management practices for urban agriculture with the aim of controlling kale diseases according to the climate conditions in the region.

Resumo Este artigo investiga relações entre a incidência de câncer de colo de útero (ICC) e os componentes e indicadores de qualidade da água nos municípios do Mato Grosso do Sul, entre 2014 e 2017, por correlação estatística (Determinante de Pearson) e espacial (agrupamentos por k-médias). Houve maior resposta estatística de ICC em relação à tarifa média dos serviços de abastecimento praticado (-36,28%) e de água (-34,15%); à quantidade de suas interrupções sistemáticas (28,3%) e paralizações (22,28%); ao consumo médio per capita de água (20,74%) e à quantidade de serviços executados (-17,98%), todas as respostas sob p-valor ≤ 0,001. Em Costa Rica, cidade sob maior ICC média, os agrupamentos espaciais identificaram maior efeito daquelas interrupções (z-valor = 8,741) e das paralizações (z = 7,6097); enquanto em Rochedo, também sob alta ICC, houve maior efeito à incidência de análises com resultados fora do padrão para coliformes totais (z = 8,6803) e turbidez (z = 5,7427), sob correlação estatística de 12,05% (p-valor = 0,032) e 15,18% (p-valor = 0,007), respectivamente. Dados do SISAGUA revelaram a presença de coliformes e de altos níveis de turbidez, por exemplo, em Antônio João e Tacuru, cidades sob altas ICC médias. Recomenda-se maiores investigações sobre as relações aqui apresentadas entre ICC e água.

Abstratct This article investigates relationships between the incidence of cervical cancer (CCI) and the water components and quality indicators, in the municipalities of Mato Grosso do Sul, between 2014 and 2017, by statistical (Pearson’s Determinant) and spatial (k-means Clustering) correlation. There was a greater statistical response of CCI in relation to the average tariff of the practiced supply (−36.28%) and water (−34.15%) services; the number of their systematic interruptions (28.3%) and outages (22.28%); the average per capita consumption of water (20.74%); and the number of services performed (−17.98%), all answers under p-value ≤ 0.001. In Costa Rica, city with the highest average CCI, the spatial clustering identified a greater effect of those interruptions (z-value = 8.741) and outages (z = 7.6097); whereas, in Rochedo, also under high CCI, the analyses showed greater effect with non-standard results for total coliforms (z = 8.6803) and turbidity (z = 5.7427), under a statistical correlation of 12.05% (p-value = 0.032) and 15.18% (p-value = 0.007), respectively. Data from SISAGUA revealed the presence of coliforms and high levels of turbidity, for example, in Antônio João and Tacuru, cities with high average ICC. We recommend further investigation into the relationships presented here between CCI and water.

Resumo Este artigo investiga relações entre a incidência de câncer de colo de útero (ICC) e os componentes e indicadores de qualidade da água nos municípios do Mato Grosso do Sul, entre 2014 e 2017, por correlação estatística (Determinante de Pearson) e espacial (agrupamentos por k-médias). Houve maior resposta estatística de ICC em relação à tarifa média dos serviços de abastecimento praticado (-36,28%) e de água (-34,15%); à quantidade de suas interrupções sistemáticas (28,3%) e paralizações (22,28%); ao consumo médio per capita de água (20,74%) e à quantidade de serviços executados (-17,98%), todas as respostas sob p-valor ≤ 0,001. Em Costa Rica, cidade sob maior ICC média, os agrupamentos espaciais identificaram maior efeito daquelas interrupções (z-valor = 8,741) e das paralizações (z = 7,6097); enquanto em Rochedo, também sob alta ICC, houve maior efeito à incidência de análises com resultados fora do padrão para coliformes totais (z = 8,6803) e turbidez (z = 5,7427), sob correlação estatística de 12,05% (p-valor = 0,032) e 15,18% (p-valor = 0,007), respectivamente. Dados do SISAGUA revelaram a presença de coliformes e de altos níveis de turbidez, por exemplo, em Antônio João e Tacuru, cidades sob altas ICC médias. Recomenda-se maiores investigações sobre as relações aqui apresentadas entre ICC e água.

Abstratct This article investigates relationships between the incidence of cervical cancer (CCI) and the water components and quality indicators, in the municipalities of Mato Grosso do Sul, between 2014 and 2017, by statistical (Pearson’s Determinant) and spatial (k-means Clustering) correlation. There was a greater statistical response of CCI in relation to the average tariff of the practiced supply (−36.28%) and water (−34.15%) services; the number of their systematic interruptions (28.3%) and outages (22.28%); the average per capita consumption of water (20.74%); and the number of services performed (−17.98%), all answers under p-value ≤ 0.001. In Costa Rica, city with the highest average CCI, the spatial clustering identified a greater effect of those interruptions (z-value = 8.741) and outages (z = 7.6097); whereas, in Rochedo, also under high CCI, the analyses showed greater effect with non-standard results for total coliforms (z = 8.6803) and turbidity (z = 5.7427), under a statistical correlation of 12.05% (p-value = 0.032) and 15.18% (p-value = 0.007), respectively. Data from SISAGUA revealed the presence of coliforms and high levels of turbidity, for example, in Antônio João and Tacuru, cities with high average ICC. We recommend further investigation into the relationships presented here between CCI and water.

Resumo O objetivo desta revisão é discutir o planejamento digital e o uso da tecnologia guiada em Endodontia. A complexidade e variabilidade da anatomia dos canais radiculares, em conjunto com o desafio no processo de sanificação e controle de micro-organismos representam fatores de risco ao fracasso após o tratamento dos canais radiculares infectados. O aprimoramento técnico-científico e os avanços tecnológicos tem possibilitado uma melhor previsibilidade de resultados nos procedimentos terapêuticos. O desenvolvimento de ferramentas de trabalho eficientes e modernas proporcionou tratamentos endodônticos seguros, com menor tempo clínico operacional e maior conforto ao paciente. A endodontia digital incorporou diferentes ferramentas e desenvolveu suas próprias, avançando ainda mais na resolução de casos complexos. A cópia fiel da anatomia interna proporcionada pelo avanço dos aparelhos e softwares de TCFC, associada aos recursos digitais de planejamento e impressão 3D possibilitaram o surgimento da endodontia guiada. Esta técnica é utilizada em diferentes etapas do tratamento endodôntico, com indicações específicas e maior previsibilidade de resultados. Este estudo revisou criticamente o potencial de aplicação clínica da técnica de acesso guiado, e os passos operatórios para sua realização de forma segura no manejo de casos endodônticos complexos. As principais indicações da Endodontia Guiada incluem o acesso a canais radiculares calcificados; as cirurgias parendodônticas em áreas de difícil acesso; a remoção de pinos de fibra de vidro; e o acesso a dentes com anomalias de desenvolvimento. Em síntese, a endodontia guiada é uma técnica precisa, eficaz e de fácil aplicação clínica. Esta técnica representa a incorporação dos recursos tecnológicos e planejamentos digitais do Endodontista, dando maior previsibilidade aos casos em que é aplicada na prática clínica.

Abstract The aim of this review is to discuss the digital planning and the use of guided technology in Endodontics. The complexity of the root canals anatomy and the challenges in the microorganism’s control represent risk factors for failure after the infected root canal’s treatment. Scientific improvements associated with technological advances have enabled better predictability of therapeutic procedures results. The development of efficient and modern devices provided safer root canal treatments, with shorter clinical visits and greater patient comfort. Digital endodontics incorporated different tools and developed its own, advancing even further in resolving complex cases. The faithful copy of the internal anatomy provided by the advancement of CBCT devices and software’s, associated with the digital resources of 3D planning and printing, enabled the advent of guided endodontics. This technique is used at different stages of endodontic treatment, with specific indications and greater result predictability. Therefore, this study critically reviewed the potential clinical application of this guided access technique, and the operative steps for its safe performance in managing complex endodontic cases. The main indications are accessing calcified root canals, performing endodontic surgeries in difficult access areas, removing fiberglass posts, and accessing teeth with developmental anomalies. In summary, guided endodontics has been a precise strategy, effective, safe, and clinically applicable. This procedure represents incorporating technological resources and digital planning in the Endodontist clinical practice, increasing predictability to complex cases.

SUMMARY OBJECTIVE: A surgery is essential for the management of early endometrial carcinoma. Due to the comorbidities associated with the disease, the complications of surgery are common. Laparoscopic surgery may reduce surgical complications but also have oncological risks. We aimed to compare recurrence and overall survival (OS) associated with laparoscopy and laparotomy for early endometrial cancer. METHODS: We included women treated for presumed early endometrial carcinoma at the Clinics Hospital of Ribeirão Preto Medical School from January 1998 to December 2017. We designed a 1:2 propensity score-matched case-control and compared the patients’ characteristics, short-term outcomes, recurrence, and OS. RESULTS: A total of 252 women were included in this study, 168 underwent laparotomy, and 84 underwent laparoscopy. The two groups were well balanced according to most of the variables, and obesity was a characteristic of patients in both groups. Laparoscopy was associated with increased surgical time (194.7 min vesus 165.6 min; p<0.001) and reduced rate of surgical complications (6.5% versus 0; p=0.038). Laparoscopic surgery was not associated with the risk of tumor recurrence (HR: 0.41, 95%CI 0.14-1.19, p=0.100) or all-cause mortality (HR: 0.49, 95%CI 0.18-1.35, p=0.170). CONCLUSION: Laparoscopy was safe in terms of oncological outcomes and was associated with a lower rate of surgical complications. Our data support the use of minimally invasive surgery as the preferential approach in the management of early endometrial carcinoma.

Nesse artigo, utilizou-se o Módulo Python Uncertainties no desenvolvimento dos códigos para efetuar os cálculos de incertezas experimentais, tanto para a diferença de potencial, quanto para as correntes elétricas. Para esse fim, foi desenvolvido um protótipo experimental que demonstra o efeito fotovoltaico a partir da utilização de materiais de baixo custo e, por conseguinte, com o objetivo de observar e quantificar as referidas grandezas físicas envolvidas. Além disso, foi realizada uma análise qualitativa e quantitativa do efeito fotovoltaico, assim como da Primeira Lei de Ohm. Nessa perspectiva, o protótipo foi associado a um multímetro, que permitiu a aferição direta da diferença de potencial, cujo o valor principal foi de (0,4582 ± 0,0004) V, quando incidido um feixe laser em um fotodiodo presente em um transistor 2N3055, mas também os valores das correntes elétricas, sendo os valores principais de (81,7100 ± 0,1118) μA e (1,9990 ± 0,0320) μA, quando se utilizou dos resistores ôhmicos de 1,0 kΩ e 220,0 kΩ, respectivamente. Por fim, é importante salientar que essa proposta de artigo objetiva a anexação da computação física no cotidiano escolar, assim como promover o despertar científico na confecção de instrumentações específicas para o ensino-aprendizagem de física.

In this article, the Python Uncertainties Module was used in the development of the codes to perform the calculations of experimental uncertainties, both for the potential difference and for the electric currents. For this purpose, an experimental prototype was developed that demonstrates the photovoltaic effect from the use of low-cost materials and, therefore, with the objective of observing and quantifying the referred physical quantities involved. In addition, a qualitative and quantitative analysis of the photovoltaic effect was carried out, as well as of Ohm’s First Law. In this perspective, the prototype was associated with a multimeter, that allowed direct measurement of the potential difference, whose main value was (0,4558 ± 0,0004) V, when a laser beam was focused on a photodiode present in a 2N3055 transistor, but also the values of the electric currents, the main values being (81,7100 ± 0,1118) μA and (1,9990 ± 0,0320) μA, when the ohmic resistors of 1,0 kΩ and 220,0 kΩ were used, respectively. Finally, it is important to note that this article proposal aims at the annexation of physical computing in school routine, as well as promoting scientific awakening in the manufacture of specific instruments for teaching and learning physics.

RESUMO Os métodos de treinamento são ferramentas diferenciais para melhorar o desempenho tático-técnico de equipes. O objetivo do presente estudo foi analisar o efeito de seis semanas na aprendizagem/desempenho segundo a utilização de dois métodos. Participaram 16 atletas masculinos. O estudo teve duração de dez semanas, dividido em três fases principais: avaliações pré, intervenção e avaliações pós. Nas fases pré e pós foram realizadas avaliações do desempenho tático-técnico e a tomada de decisão no ataque. A intervenção foi realizada com três sessões de treinamento por semana no período de seis semanas para cada grupo de acordo com o método (analítico e situacional) e ao final da sessão foram realizados jogos tradicionais e/ou adaptados. Na eficácia das ações com o método de treinamento situacional, a recepção (p= 0,014) e o levantamento (p= 0,004) apresentaram aumento entre os momentos pré vs. pós. No coeficiente de desempenho com o método de treinamento situacional, a recepção (p= 0,025) e o levantamento (p= 0,020) também apresentaram aumento entre os momentos pré vs. pós. E na tomada de decisão do ataque, o grupo situacional (p= 0,019) promoveu adaptações superiores ao método análitico. Conclui-se que o método situacional promove aos atletas iniciantes de voleibol de praia aprendizado em relação aos fundamentos de recepção de saque e levantamento, como também para a tomada de decisão do ataque.

ABSTRACT Training methods are differential tools for improving the tactical and technical performance of teams. The aim of the presente study was to analyze the effect of six weeks on learning/performance using two methods. 16 male athletes participated. The study lasted ten weeks, divided into three main phases: pre, intervention and post evaluations. In the pre and post phases were performed evaluations of tactical-technical performance and decision making in the attack. The intervention was performed with three training sessions per weeks for each group according to the method (analytical and situational) and at the end of the session traditional and/or adapted games were performed. In the effectiveness of actions with the situational training method, the reception (p= 0.014) and the survey (p= 0.004) showed an increase between the pre vs. pos moment. In the performance coefficient with the situational training method, the reception (p= 0.025) and the survey (p= 0.020) also showed an increase between the pre vs. pos moment. And in the decision making of the attack, the situational group (p= 0.019) promoted superior adaptations to the analytical method. It is concluded that the situational method promotes beginners to beach volleyball learning in relation to the fundamentals of receiving service and lifting, as well as for decision making of the attack.