ABSTRACT Background: An external quality assessment on the identification of triatomines within the laboratory network in the state of Rondônia. Methods: Seven laboratories participated in this evaluation. Each was provided with support materials and nine insects from the Hemiptera order for identification. Results: All samples were accurately identified at the species level. However, correct sex identification was achieved for only 79% of the samples. The most significant challenges were encountered in determining the sex of predators, phytophagous species, Rhodnius robustus, and Rhodnius pictipes. Conclusions: The identified shortcomings can inform enhancements in vector control programs for Chagas disease. Background Rondônia Methods evaluation Results level However 79 predators robustus pictipes Conclusions disease 7

Resumo Objetivo: Avaliar a qualidade da identificação de triatomíneos na rede de laboratórios do estado de Pernambuco, Brasil. Métodos: Os nove laboratórios participantes receberam material de apoio com as chaves dicotômicas e um painel composto por sete insetos triatomíneos conhecidos no estado, para identificação da situação do espécime no recebimento (estrutura completa ou danificada), espécie e sexo. Resultados: Nove laboratórios de 12 aderiram ao estudo. A proporção de acerto para identificação do sexo foi de 56/63, e para espécie, 45/63, não apresentando relação direta com a ocorrência de danos nas estruturas morfológicas durante o transporte dos insetos. Para Panstrongylus megistus, houve acerto em todos os espécimes (9/9), enquanto para espécies do gênero Rhodnius a proporção foi menor (3/9). Conclusão: Apesar do bom desempenho na identificação entomológica, as fragilidades observadas poderão orientar ações para melhoria na rede de laboratórios e serão essenciais para os programas de controle vetorial da doença de Chagas.

Abstract Objective: To assess the quality of triatomine identification in the laboratory network of the state of Pernambuco, Brazil. Methods: The participating laboratories received support material with dichotomous keys and a panel made up of seven triatomine insects known in the Pernambuco, in order to identify specimen status on receipt (intact or damaged structure), as well as species and sex. Results: Nine out of 12 laboratories took part of the study. The proportion of correct answers was 56/63 for sex identification, and 45/63, for species. The answers did not present a direct relationship with occurrence of damage to morphological structures during insect transportation. Panstrongylus megistus identification was correct for all specimens (9/9 laboratories), while correct identification of species of the Rhodnius genus was the lowest (3/9 laboratories). Conclusion: Despite the good performance in entomological identification, the weaknesses observed may guide improvements in the laboratory network and will be essential for Chagas disease vector control programs.

Resumen Objetivo: Evaluar la calidad de la identificación de triatominos en la red de laboratorios del estado de Pernambuco, Brasil. Métodos: Los nueve laboratorios participantes recibieron material de apoyo con claves dicotómicas y un panel conformado por siete insectos triatominos conocidas en el estado, para identificar la situación del espécimen al recibirlo (estructura completa o dañada), especie y sexo. Resultados: La adherencia al laboratorio fue del 9/12. La proporción de aciertos para la identificación del sexo fue del 56/63, y para las especies 45/63, no mostraron relación directa con la ocurrencia de daños en las estructuras morfológicas durante el transporte de insectos. Solo Panstrongylus megistus logró el 9/9 de precisión, mientras que para las especies del género Rhodnius fue el más pequeño (3/9). Conclusión: A pesar del buen desempeño en identificación entomológica, la evaluación indicó debilidades que permitirán implementar acciones correctivas para mejorar la red de laboratorios, esenciales para los programas de control vectorial de la enfermedad de Chagas.

Resumo Objetivo: Avaliar a qualidade da identificação de triatomíneos na rede de laboratórios do estado de Pernambuco, Brasil. Métodos: Os nove laboratórios participantes receberam material de apoio com as chaves dicotômicas e um painel composto por sete insetos triatomíneos conhecidos no estado, para identificação da situação do espécime no recebimento (estrutura completa ou danificada), espécie e sexo. Resultados: Nove laboratórios de 12 aderiram ao estudo. A proporção de acerto para identificação do sexo foi de 56/63, e para espécie, 45/63, não apresentando relação direta com a ocorrência de danos nas estruturas morfológicas durante o transporte dos insetos. Para Panstrongylus megistus, houve acerto em todos os espécimes (9/9), enquanto para espécies do gênero Rhodnius a proporção foi menor (3/9). Conclusão: Apesar do bom desempenho na identificação entomológica, as fragilidades observadas poderão orientar ações para melhoria na rede de laboratórios e serão essenciais para os programas de controle vetorial da doença de Chagas.

Abstract Objective: To assess the quality of triatomine identification in the laboratory network of the state of Pernambuco, Brazil. Methods: The participating laboratories received support material with dichotomous keys and a panel made up of seven triatomine insects known in the Pernambuco, in order to identify specimen status on receipt (intact or damaged structure), as well as species and sex. Results: Nine out of 12 laboratories took part of the study. The proportion of correct answers was 56/63 for sex identification, and 45/63, for species. The answers did not present a direct relationship with occurrence of damage to morphological structures during insect transportation. Panstrongylus megistus identification was correct for all specimens (9/9 laboratories), while correct identification of species of the Rhodnius genus was the lowest (3/9 laboratories). Conclusion: Despite the good performance in entomological identification, the weaknesses observed may guide improvements in the laboratory network and will be essential for Chagas disease vector control programs.

Resumen Objetivo: Evaluar la calidad de la identificación de triatominos en la red de laboratorios del estado de Pernambuco, Brasil. Métodos: Los nueve laboratorios participantes recibieron material de apoyo con claves dicotómicas y un panel conformado por siete insectos triatominos conocidas en el estado, para identificar la situación del espécimen al recibirlo (estructura completa o dañada), especie y sexo. Resultados: La adherencia al laboratorio fue del 9/12. La proporción de aciertos para la identificación del sexo fue del 56/63, y para las especies 45/63, no mostraron relación directa con la ocurrencia de daños en las estructuras morfológicas durante el transporte de insectos. Solo Panstrongylus megistus logró el 9/9 de precisión, mientras que para las especies del género Rhodnius fue el más pequeño (3/9). Conclusión: A pesar del buen desempeño en identificación entomológica, la evaluación indicó debilidades que permitirán implementar acciones correctivas para mejorar la red de laboratorios, esenciales para los programas de control vectorial de la enfermedad de Chagas.

Abstract INTRODUCTION: This short communication presents a novel report on the occurrence of Panstrongylus rufotuberculatus in the Brazilian state of Rondônia. METHODS: Two specimens were collected inside dwellings and identified using dichotomous keys. RESULTS: The present study showed the extensive geographic distribution of P. rufotuberculatus and the increased number of species in the state of Rondônia. CONCLUSIONS: This new record of P. rufotuberculatus is important for understanding the epidemiology of Chagas disease because this species is found naturally infected with Trypanosoma cruzi. Studies on the ecology, biology, and vector-host-parasite interactions of this species are essential for surveillance programs.

RESUMO Objetivo pesquisar a atividade elétrica do músculo orbicular da boca em quatro diferentes tarefas isométricas e indicar a mais apropriada para utilização como referência na normalização do sinal elétrico do músculo orbicular da boca pela contração voluntária máxima. Métodos participaram do estudo 22 indivíduos, sendo dez do sexo masculino e 12 do feminino, com idades entre 20 e 33 anos. A atividade elétrica das porções superior e inferior do músculo orbicular da boca foi registrada durante a realização das seguintes tarefas isométricas: protrusão labial com fechamento, protrusão labial sem fechamento, contrarresistência com a placa de resistência labial e preensão de lábios. A ordem de realização das tarefas foi randomizada entre os participantes. Os dados foram comparados entre as tarefas, para cada seguimento labial. Resultados no segmento marginal superior, a tarefa com maior média e mediana de amplitude foi preensão labial, embora sem diferença significativa entre tarefas. O menor coeficiente de variação foi obtido na tarefa de contrarresistência com a placa de resistência labial. No segmento marginal inferior, a tarefa com maior média e mediana de amplitude foi protrusão labial com fechamento, que também teve o menor coeficiente de variação e diferença significativa entre as tarefas protrusão labial com fechamento e preensão, sendo os maiores valores obtidos na primeira. Conclusão indica-se como referência para a normalização do sinal elétrico do músculo orbicular da boca, pela contração voluntária máxima, a tarefa de protrusão labial com fechamento.

ABSTRACT Purpose To verify the electrical activity of the orbicularis oris in four different isometric tasks and to indicate the most appropriate task for use as a reference in the normalization of the electrical signal of the orbicularis oris muscle by maximum voluntary contraction. Methods Twenty-two individuals participated in the study, of which 10 were male and 12 were female, with ages ranging from 20 to 33 years. The electrical activity of the upper and lower parts of orbicularis oris muscle was recorded during the following isometric tasks: lip protrusion with bilabial closure; lip protrusion without bilabial closure; the counter resistance using the plate of labial resistance and lips prehension. The order of the tasks was randomized among participants. Data were compared between tasks for each lip part. Results in the upper marginal segment, the task with the highest mean and median amplitude was lip prehension, although there was no significant difference between tasks. The lowest coefficient of variation was obtained in the counter resistance task with the plate of labial resistance. In the lower marginal segment, the task with the highest mean and median amplitude was labial protrusion with lips closure, which also had the lowest coefficient of variation. In this segment, there was a significant difference between labial protrusion with lips closure and prehension, with the highest values obtained in the first task. Conclusion lip protrusion with bilabial closure is suggested as reference for the normalization of the electrical signal of the orbicularis oris muscle, by maximum voluntary contraction.

INTRODUÇÃO: O presente estudo apresenta uma análise descritiva da ocorrência de triatomíneos e seus índices de infecção natural por Trypanosoma no Estado de Pernambuco, entre 2006 e 2007. MÉTODOS: Dados entomológicos para as espécies de triatomíneos, tais como espécimes capturados no intra e peridomicílio, e índice de infecção natural foram obtidos por meio da captura domiciliar em 147 municípios das 11 Gerências Regionais de Saúde. A pequisa foi baseada em uma amostra de insetos (100% dos infectados e 20% dos não infectados) enviados para o Laboratório Central de Pernambuco. RESULTADOS: No total, 18.029 triatomíneos foram analisados provenientes de 138 municípios. Triatoma pseudomaculata (35%), Triatoma brasiliensis (34%) e Panstrongylus lutzi (25%) foram as espécies mais capturadas. Estas espécies também apresentaram ampla distribuição geográfica no estado. Panstrongylus megistus, Triatoma petrocchiae, Triatoma melanocephala, Triatoma sordida, Rhodnius nasutus, Rhodnius neglectus e Triatoma infestans apresentaram distribuição geográfica mais restrita e menores valores de abundância relativa. A pesquisa parasitológica mostrou que 8,8% dos triatomíneos estavam infectados por flagelados morfologicamente similares a Trypanosoma cruzi e 91,3% deles foram capturados no interior das habitações em 113 municípios. P. lutzi apresentou as maiores taxas de infecção natural. CONCLUSÕES: Após o controle do T. infestans, as espécies sinantrópicas T. brasiliensis, T. pseudomaculata e P. lutzi mantêm o risco de transmissão do T. cruzi ao homem no Estado de Pernambuco. Estas espécies são amplamente distribuídas e espécimes infectados foram encontrados dentro das casas. Assim, nossos resultados recomendam reforçar a vigilância e controle vetorial da doença de Chagas em Pernambuco.

INTRODUCTION: The present study shows a descriptive analysis of triatomine occurrence and its natural Trypanosoma infection rates in the state of Pernambuco, Brazil, between 2006 and 2007. METHODS: Entomological data for the species, such as specimens captured in both intra and peridomiciles and natural infection index, were obtained via domiciliary capture in 147 municipalities from 11 Regional Managements of Health. The database was obtained from a sample of insects (100% infected and 20% non-infected) sent to the Central Laboratory of Pernambuco. RESULTS: A total of 18,029 triatomines were analyzed from 138 municipalities of the state. Triatoma pseudomaculata (35%), Triatoma brasiliensis (34%), and Panstrongylus lutzi (25%) were the most captured species. These species also showed a widespread geographical distribution in the state. Panstrongylus megistus, Triatoma petrocchiae, Triatoma melanocephala, Triatoma sordida, Rhodnius nasutus, Rhodnius neglectus, and Triatoma infestans showed more limited geographical distribution and lower relative abundance. The parasitological research showed that 8.8% of the triatomines were naturally infected with flagellates morphologically similar to Trypanosoma cruzi and 91.3% of them were captured inside houses in 113 municipalities. P. lutzi showed the highest rates of natural infection. CONCLUSIONS: After the control of T. infestans, synanthropic species, such as T. brasiliensis, T. pseudomaculata, and P. lutzi, maintain the risk of T. cruzi transmission to humans in the state of Pernambuco. These species are widely distributed, and infected specimens have been found inside houses. Thus, an enhanced surveillance and vector control of Chagas disease is recommended in Pernambuco.

Os autores descreveram as características morfológicas de Triatoma klugi Carcavallo, Jurberg, Lent & Galvão, 2001 pertencente ao grupo de espécies que compõem o "complexo T. oliveirai". Até o presente tem sido difícil separar essas espécies com base nas características ninfais, o que justifica o desenvolvimento deste trabalho. Os espécimes foram coletados em frestas de rochas no morro Malavok na localidade de Linha Brasil, município de Nova Petrópolis, no Estado do Rio Grande do Sul. O local de captura dos triatomíneos situa-se entre 700 e 800 m de altitude (29º18'38''S, 51º04'57''W). Juntamente com T. oliveirai (Neiva, Pinto & Lent, 1939), espécie morfologicamente mais próxima, são as únicas do complexo que não foram encontradas, até o momento, no Estado do Mato Grosso, Mato Grosso do Sul e Goiás, Brasil. Em condições experimentais, já foi testada e comprovada a sua susceptibilidade à infecção pelo Trypanosoma cruzi (Chagas, 1909) e Trypanosoma rangeli (Tejera, 1920). A análise comparativa das ornamentações do exocório dos ovos e de três estádios ninfais de T. klugi por microscopia óptica e microscopia eletrônica de varredura mostrou algumas particularidades morfológicas, com destaque para: a face ventral da cabeça, o sulco estridulatório e os últimos segmentos abdominais (IX, X - futura genitália e XI - tubo anal). Esses dados contribuem para a ampliação dos parâmetros diferenciais visando à diagnose de T. klugi durante o seu desenvolvimento ninfal.

The authors had described the morphologic characteristics of Triatoma klugi Carcavallo, Jurberg, Lent & Galvão, 2001 to the group of species belonging to "T. oliveirai complex". Until the moment it has been difficult to separate on the nymphs characteristics basis these species, what it justifies the development of this work. The specimens had been collected in openings of rocks in the Malavok mount in the locality of Linha Brazil, city of Nova Petrópolis, in the State of Rio Grande do Sul. The local of capture of the Triatominae is placed enters 700 and 800 m of altitud (29º18'38''S, 51º04'57''W). Together with T. oliveirai (Neiva, Pinto & Lent, 1939), its morphologically related species, is the only ones of the complex that had not been found, until the moment, in the State of Mato Grosso, Mato Grosso do Sul and Goiás, Brazil. In experimental conditions, already it was tested and proven its susceptibilities to the infection for Trypanosoma cruzi (Chagas, 1909) and Trypanosoma rangeli (Tejera, 1920). The comparative analysis of the ornaments of the exocorium of eggs and of three nymphs of T. klugi by optic microscopy and scanning electron microscopy showed some morphologic particularities, with prominence for: the ventral face of the head, the stridulatory sulcus and the last abdominal segments (IX, X - future genitalia and XI - anal duct). These data contribute for the magnifying of the distinguishing parameters aiming at diagnosis of T. klugi during its nymphal development.

Uma nova espécie de Rhodnius é descrita e ilustrada baseada em um exemplar macho do nordeste brasileiro. O espécime foi encontrado morto e ressecado. Esta espécie pode ser separada da espécie afim, R. paraensis Sherlock, Guitton & Miles, pelo seu maior tamanho, pelo aspecto geral de coloração do corpo, pela cor uniforme das pernas, pelas diferentes proporções entre as estruturas morfológicas da cabeça e dos segmentos do rostro; ainda, na genitália masculina, pelo aspecto do processo mediano do pigóforo, do falosoma e de seu processo.

A new species of Rhodnius is described and illustrated from a male specimen from northeastern Brazil. The specimen was found died and dried. This species can be distinguished from its similar congener R. paraensis Sherlock, Guitton & Miles by the greater size, the general aspect of the color of the body, by the uniform color of the legs, different proportions between the morphological structures of the head and the segments of the rostrum; also, in the male genitalia by the aspect of median process of pygophore, dorsal phallotheca plate and phallotheca process.

Os autores utilizaram a técnica de precipitina para identificar as fontes alimentares de Panstrongylus lutzi (Neiva & Pinto, 1923) em 20 municípios do Estado do Ceará, Brasil. Os resultados detectaram a presença de sangue de oito diferentes fontes sanguíneas e alimentações mistas, demonstrando que Panstrongylus lutzi é uma espécie eclética. Altas taxas de infecção por tripanosomatídeos foram detectadas.

The authors used precipitin technique to detect the feeding sources of Panstrongylus lutzi (Neiva & Pinto, 1923) in 20 municipalities of Ceará State, Brazil. The results detected the presence of blood from eight different blood sources and mixed feedings, demonstrating that Panstrongylus lutzi is an eclectic species. High infection rates for Trypanosoma like-cruzi were detected.

Eggs and all nymphs of these species were studied employing light microscopy (LM) and scanning electron microscopy (SEM). The major differences observed by LM in the eggs were related to the presence and the distribution of pores on the surface of their chorion. Morphological differences among three nymphal stages (1st, 3rd, and 5th) development of each species were observed. The differential characteristics are chromatic and in the shape of connexival spots. The ultrastructure of the ventral region of the head and the IX, X, and XI abdominal segments (anal tube) of the both species were described demonstrating morphological differences that can be used for diagnosis of the species.

Pela primeira vez todos estádios ninfais de uma espécie do gênero Belminus são descritos, baseados em espécimes da primeira geração obtida em laboratório a partir de adultos capturados em domicílios do Departamento de Cesar, Colômbia. A morfometria geométrica das cabeças sugere que as maiores mudanças no desenvolvimento pós-embrionário ocorrem entre o 1º e o 2º ínstares.

For the first time all instars of a species of the genus Belminus are described based on laboratory F1 generation specimens from adults caught in department of Cesar, Colombia. Ontogenetic morphometrics of postembryonic head development was made, suggesting that the greatest changes occur between the 1st and 2nd instars.

R. brethesi is a sylvatic species from the Amazon region; it has been incriminated as responsible for the transmission of Chagas disease in collectors of piaçaba in this region. The aim of present study was to investigate the efficiency of these insects as potential vectors of Trypanosoma cruzi. Aspects related with feeding and defecation patterns, life time, and mortality had been observed in each instar of R. brethesi. We use 5th instar nymphs to get adults virgins, after the moulting 3 groups with 6 females and 2 males each were created to obtain eggs. After hatching, 1st instar nymphs had been weighed and kept in bottles until the next moult. Insects were fed once a week in mice. Results showed that the average period of incubation was 17 days, the number of blood meal was increasing from the 1st to the 5th instar nymph with 7 (average) to become adult, a significative numbers of the defecations occurring immediately after the bloodmeals. The total percentual of mortality was 16%. This results suggests that this species presents a good exploitation of blood meals and a brief nymphal development in laboratory conditions reflecting its behavior in sylvatic environments.

Belminus herreri, originally described from specimens collected in Panama, was considered entirely silvatic until to 2000 when it was found for the first time in a domestic habitat in Colombia. In 2001, during a new search of houses in the Department of Cesar, Colombia, 121 specimens were collected. Study of their feeding sources using an ELISA test revealed that 96% of these specimens had fed on cockroaches (Blattidae). However, a small proportion of these B. herreri specimens also showed the presence of Trypanosoma cruzi in their gut contents, suggesting a possible role for these insects in the epidemiology of Chagas disease.

The subfamily Triatominae is actually represented by 137 species distributed among 6 tribes and 19 genera. Within this subfamily, the genus Panstrongylus, Berg 1879, is composed by 13 species widespread in sylvatic, peridomestic, and domestic habitats of Neotropical regions. These species are vectors of Chagas disease and consequently are found associated with its main hosts, such as birds and mammals. Interest in species of this genus has been increasing in the last few years. Reports of several authors indicate these Triatominae to invade and colonize houses, increasing their epidemiological significance. Morphometry was used in this study to investigate correlations among possible closely related species. We measured 224 specimens among 13 species through a set of metric variables of the head. The results indicated that the genus Panstrongylus seems to be homogeneous since 10 of the 14 species were shown to be closely related.

There are several specific complexes belonging to the genus Triatoma Laporte, 1832, which are generally associated to specific geographic areas. Recent publications have linked the oliveirai complex to ecosystems of Mato Grosso, which are also present in other Brazilian states and even in other bordering countries as eastern Paraguay. The study of the abundant material collected during the last years allowed the description of several new species of the oliveirai complex: T. jurbergi Carcavallo, Galvão & Lent, 1998; T. baratai Carcavallo & Jurberg, 2000 and T. klugi Carcavallo, Jurberg, Lent & Galvão, 2001. Another new species belonging to the same complex is described here as T. vandae sp.n. It originates from the state of Mato Grosso, and has been reared in the insectary of the Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Departamento de Entomologia, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro.