Analysis of variance (ANOVA)-simultaneous component analysis (ASCA) is a method of choice for factorial design studies of environmental impacts on plant metabolomes and can be used to quantitatively carry out this comparative analysis. The impacts of seven mixture design extractor systems made up of ethanol, dichloromethane, and hexane and their 1:1 binary and 1:1:1 ternary solvents for several replicate experiments (n = 3, 4, 5) were assessed using ASCA models determined from Fourier-transform infrared (FTIR), near-infrared (NIR), and UV-Vis spectroscopic measurements on yerba mate leaf extracts. This analysis considered two-factor effects: secondary sexual dimorphism (male and female plants) and cultivation systems (monoculture and agroforestry), as well as their interaction effect. The three binary solvents were found to be more efficient extractor systems for all four detectors as they found 83 main and interaction effects significant at or above the 95% confidence level compared with only 47 for the pure solvent extracts. Binary solvent extracts resulted in averages between 44.04 and 86.61% for the ASCA total effect variances compared with 40.62 to 71.07% for pure extractors. Of the 60 significant effects found for experiments with 5 repetitions 53 or 88% were obtained with only triplicate determinations. The choice of spectroscopic technique and solvent system have large impacts on metabolomic analysis results. ANOVAsimultaneous ANOVA simultaneous (ASCA ethanol dichloromethane 11 1 1: 111 1:1: n 3 4 Fouriertransform Fourier transform FTIR, FTIR , (FTIR) nearinfrared near NIR, NIR (NIR) UVVis UV Vis twofactor two factor male plants monoculture agroforestry, agroforestry agroforestry) 8 95 4404 44 04 44.0 8661 86 61 86.61 4062 40 62 40.6 7107 71 07 71.07 extractors 6 88 determinations results (FTIR (NIR 9 440 0 44. 866 86.6 406 40. 710 7 71.0 86. 71.

The effects of water-deficit stress on irrigated and unirrigated field plants of Coffea arabica L. genotype IAPAR 59 were investigated. Plant extracts were obtained following an ethanol-dichloromethane-hexane statistical mixture design. Proton nuclear magnetic resonance (1H NMR) fingerprints of the extracts were discriminated using factor analysis (FA) and hierarchical clustering techniques. Extracts from the 1:1:1 ternary mixture presented the largest discriminations compared with those from the pure solvents or their 1:1 binary mixtures. Metabolites resulting from fermentation processes and nutritional deficiencies as well as senescence and abscission precursors such as lactate, arginine and methionine were prevalent in unirrigated plants that can provoke expressive decreases in bean productivity as well as premature plant aging. Amino acids that control regulatory, physiological processes and soil salinization have higher concentrations in the irrigated plants. The NMR assignments of eighteen substances observed here were confirmed by electrospray ionization mass spectrometry.

Coffee bean chemical compositions has been extensively studied. However, there is a small amount of research on other parts of the coffee plant, including leaves. Fourier transform infrared (FTIR) spectral profiles of Coffea arabica L. cv. IAPAR 59 leaf extracts from a simplex-centroid design were studied by principal component analysis (PCA) to evaluate the effect of solvent extractor on its metabolites. PCA indicated that the extractor solvents containing ethanol were the most suitable for this study. FTIR spectra in conjunction with orthogonal signal correction and partial least squares-discrimination analysis (OSC-PLS-DA) were used to classify and discriminate the leaves of irrigated and non-irrigated plants by bands related to carbohydrates, amino acids and lipids. Leaves receiving different intensities of solar radiation were also discriminated by bands corresponding to caffeine, carbohydrates and lipids. FTIR spectral profile analyzed with chemometric tools showed to be a useful, powerful and simple procedure to discriminate coffee leaves collected from different microclimate conditions.

Ultraviolet characteristic profiles and infrared spectroscopic (FTIR) fingerprints of green bean extracts of Coffea arabica L., cv. IAPAR 59, cultivated in two planting patterns, rectangular and square, and at two different densities, 10,000 and 6,000 plants ha-1, identified as R10, R6, S10, and S6 were analyzed with principal component and hierarchical cluster analyses. A simplex centroid design for four solvents (ethanol, acetone, dichloromethane, hexane) was used for sample extraction. The largest chlorogenic acid (CGA) contents were found at the lower planting density. The dichloromethane extracts of the S10 treatment showed the highest levels of unsaponifiable lipids (cafestol and kahweol). The R6 treatment showed a slightly higher content of cafestol and kahweol. Cluster analysis of FTIR fingerprints confirmed that the CGA and caffeine levels differentiate the spatial arrangements. The FTIR fingerprints suggest that green beans from S6 and R10 were richer in lipids and the other two treatments had more sugars and proteins.

Impressões digitais de folhas de Coffea arabica expostas à luz solar e autossombreadas colhidas na mesma planta, foram usadas para determinar as variações das concentrações dos metabólitos devido a diferentes ambientes de luz e fases fenológicas. Os rendimentos dos extratos nos solventes etanol, acetona, hexano e diclorometano, bem como as suas misturas são apresentados. Os maiores rendimentos para todas as folhas autossombreadas e expostas ao sol são obtidos com misturas binárias etanol-acetona. Análise de componentes principais (PCA) dos espectros de infravermelho com transformada de Fourier (FTIR) dos extratos indicam diferenças espectrais entre 2962-2828, 1759-1543 e inferiores a 1543 cm-1 que podem ser atribuídos a maiores concentrações de ésteres de ácidos graxos ou grupo éster em triglicerídeos, cafeína, ácidos clorogênicos e carboidratos, que são mais predominantes em folhas na fase de floração. Os espectros dos picos de cromatografia líquida de alta eficiência com detector UV de arranjo de diodos (HPLC-UV-DAD) dos extratos mostraram que folhas expostas ao sol apresentam absorções mais fortes para a cafeína, ácido clorogênico e teobromina. Experimentos confirmatórios para determinar a concentração de cafeína realizados com curvas de calibração UV, mostram que a cafeína de folhas expostas ao sol são cerca de duas vezes maior que as folhas autossombreadas na fase de floração. O conhecimento da quantidade de cafeína em folhas de Coffea arabica é de importância ecológica, já que plantas expostas ao sol parecem mais estressadas do que as autossombreadas para esta espécie. As concentrações lipídicas em folhas autossombreadas são quase o dobro daquelas expostas ao sol.

Fingerprints of self-shaded and sunlight-exposed leaves of the same Coffea arabica plant were obtained to determine metabolic concentration changes owing to different light environments and phenological stages. Leaf extract yields of the ethanol, acetone, dichloromethane and hexane solvents, as well as their statistical design mixtures, are reported. Highest yields are obtained with binary 1:1 ethanol-acetone mixtures for all sun-exposed and self-shaded leaves. Principal component analysis (PCA) of Fourier transform infrared (FTIR) spectra of leaf extracts indicate spectral differences between 2962-2828, 1759-1543 and below 1543 cm-1 that can be attributed to higher concentrations of fatty acid esters or the ester group in triglycerides, caffeine, chlorogenic acids and carbohydrates that are more prevalent in leaves of flowering plants. High-performance liquid chromatography with UV diode array detector (HPLC-UV-DAD) spectra of the chromatographic peaks for the extracts showed that sun-exposed samples contain stronger absorptions for caffeine, chlorogenic acid and theobromine. Confirmatory experiments carried out with reference UV calibration curves determined caffeine contents for sun-exposed leaves that are about double those for self-shaded leaves of flowering plants. Knowledge of leaf caffeine content in Coffea arabica is of ecological importance since sun-exposed conditions seem more stressful than self-shading ones for this species. Lipid concentrations in self-shaded leaves are almost double those that were sun-exposed.

Os objetivos deste trabalho foram determinar o movimento heliotrópico dos trifólios superiores de duas cultivares de soja, BR 16 e Embrapa 48, durante o dia, em três estádios fenológicos e em dois regimes hídricos e determinar o impacto da irrigação e do movimento foliar diário nas características agronômicas e na produção de grãos. Movimentos heliotrópicos foram determinados a cada hora, em três estádios fenológicos: V4-V6, V7-V10 e R5, em parcelas irrigadas e não irrigadas. Para cada estágio, a elevação do folíolo e o azimute foram medidos por hora. Em índices de área foliar (IAF) baixo e médio (V4-V6 e V7-V10), o diaheliotropismo foi levemente mais intenso e frequente nas plantas sem irrigação do que nas irrigadas, e somente nas primeiras horas matinais e últimas horas da tarde. Em R5 (alto IAF), o paraheliotropismo dos trifólios superiores foi predominante e mais intenso em plantas não irrigadas. Os movimentos heliotrópicos se correlacionaram com o ganho de carbono mas não com o ambiente (intensidade de luz ou temperatura), para medidas às 11 horas. 'Embrapa 48' apresenta maior paraheliotropismo do que 'BR 16', em plantas com alto IAF enquanto 'BR 16' apresenta menor plasticidade nas respostas heliotrópicas nas parcelas irrigadas. Apesar de diferenças no heliotropismo, o genótipo e disponibilidade de água não influenciaram a produção de grãos.

The objectives of this work were to determine the heliotropic movements of the upper trifoliates for two soybean cultivars, BR 16 and Embrapa 48, during a daily cycle, in three phenological stages and two water regimes, and to estimate the impact of irrigation and daily leaflet movements on agronomic characteristics and grain yield. Heliotropic movements were studied in three phenological stages: V4-V6, V7-V10, and R5 in irrigated and non-irrigated plots. For each stage, the leaflet elevation and azimuth were measured hourly. Under a low (V4-V6 stage) and mid (V7-V10 stage) leaf area index (LAI) the diaheliotropism was slightly more frequent and intensive in non-irrigated than in irrigated plants, only at early morning and late afternoon hours. At R5 stage (high LAI) the paraheliotropism of superior trifoliates was predominant and more intensive in non-irrigated plants. The heliotropic movements are correlated to carbon gain, but not to environment (light intensity or temperature), for measurements at 11h. 'Embrapa 48' expresses greater paraheliotropism than 'BR 16' at high LAI, while 'BR 16' displays lower heliotropic plasticity under irrigation. In spite of significant heliotropic differences, genotype and water availability treatments did not influence the final grain yield.

O objetivo deste trabalho foi construir maquetes de plantas de erva-mate em vários estágios de desenvolvimento com o uso do programa InterpolMate, e de computar a fotossíntese a partir de estrutura interpolada. Maquetes de erva-mate foram construídas com o software VPlants para três estágios de crescimento. Foram consideradas plantas do sexo masculino e feminino, cultivadas em dois ambientes distintos: monocultura e sub-bosque. Para modelar a arquitetura dinâmica 3D, de plantas individuais de erva-mate, durante o período bienal de crescimento entre duas podas, foram utilizadas informações coletadas em 38 datas do desenvolvimento dos galhos. Os valores estimados a partir das maquetes, incluindo dimorfismo sexual e fotossíntese foliar, são muito próximos aos observados em campo. Contudo, essa semelhança foi limitada às reconstruções que incluíram unidades de crescimento de conjuntos de dados originais. A modelagem da dinâmica de crescimento possibilita estimativas de fotossíntese de plantas inteiras da erva-mate, o que é dificilmente mensurável no campo. O software InterpolMate é eficiente na construção de maquetes de erva-mate.

The objective of this work was to build mock-ups of complete yerba mate plants in several stages of development, using the InterpolMate software, and to compute photosynthesis on the interpolated structure. The mock-ups of yerba-mate were first built in the VPlants software for three growth stages. Male and female plants grown in two contrasting environments (monoculture and forest understory) were considered. To model the dynamic 3D architecture of yerba-mate plants during the biennial growth interval between two subsequent prunings, data sets of branch development collected in 38 dates were used. The estimated values obtained from the mock-ups, including leaf photosynthesis and sexual dimorphism, are very close to those observed in the field. However, this similarity was limited to reconstructions that included growth units from original data sets. The modeling of growth dynamics enables the estimation of photosynthesis for the entire yerba mate plant, which is not easily measurable in the field. The InterpolMate software is efficient for building yerba mate mock-ups.

Este trabalho teve como objetivo indicar algumas possíveis manipulações ecofisiológicas, com base em experimentos de arquitetura de plantas. Plantas de erva-mate, seringueira e videira foram caracterizadas no campo e no laboratório, e suas maquetes 3D foram reconstruídas com os programas V-Plants e PlantGLViewer. Indicações sobre a análise de crescimento e impactos ambientais, com a aplicação de diversas simulações, foram discutidas.

The objective of this work was to indicate some possible ecophysiological manipulations, based on experiments of plant architectural traits. Yerbamate, rubber tree and grapevine plants were measured in field and laboratory, and their 3D mockups were constructed using V-Plants and PlantGLViewer softwares. Indications about growth and environmental impact analysis were discussed, applying diverse simulations in modeling process.

Mediu-se a intensidade de amargor da bebida preparada a partir de folhas da erva-mate (Ilex paraguariensis) de diversas idades, situadas em duas posições na copa (interior e ponteiras), produzidas por plantas masculinas e femininas cultivadas na floresta antropizada e em monocultura. As trocas gasosas foliares, a temperatura de folhas e a densidade de fluxo de fótons fotossinteticamente ativos também foram medidas. Com isso verificou-se que a idéia corrente de que o sombreamento está diretamente relacionado ao sabor suave do chimarrão é completamente equivocada, já que as competições inter- e intra-específicas por luz e auto-sombreamento provocaram o mesmo efeito no sabor do chimarrão, isto é, todos os tipos de sombreamento resultaram em sabor mais amargo de folhas processadas do de pleno sol. Assim, as folhas de plantas cultivadas em monocultura mostraram-se menos amargas do que aquelas de plantas manejadas sob a floresta. Além disso, as folhas de ponteiras (mais jovens) da monocultura originaram o chimarrão mais suave e os machos produziram folhas menos amargas que as fêmeas em ambos ambientes luminosos (floresta e monocultura). O sabor mostrou-se relacionado à fotossíntese, à transpiração e à temperatura de folha. Em sombreamento a modificação de sabor foi direcionada ao aumento de amargor e, aparentemente, relacionada ao decréscimo da temperatura de folhas e da transpiração na escala diurna.

The bitterness intensity of beverage prepared from the leaves produced on the males and females of yerba mate (Ilex paraguariensis), grown in the forest understory and monoculture, was evaluated. The leaves were grouped by their position (in the crown and on the branch tips) and by the leaf age. The leaf gas exchange, leaf temperature and photosynthetic photon flux density were observed. Inter and intra-specific competition for light and self-shading showed the same effect on yerba mate beverage taste. All the shading types resulted in bitterer taste of the processed yerba mate leaves compared to the leaves originated under the direct sun exposure. The leaves from the plants grown in the monoculture showed less bitterness than those grown in the forest understory. This conclusion was completely opposite to the conventionally accepted paradigm of the yerba mate industries. The leaves from the tips (younger leaves) of the plants grown in the monoculture resulted a beverage of softer taste; the males produced less bitter leaves in any light environment (forest understory or in the crown in monoculture). The taste was related to the photosynthetic and transpiration rate, and leaf temperature. Stronger bitterness of the leaves provided from the shade conditions was related to the decreased leaf temperature and transpiration in the diurnal scale.

A influência de diferentes fontes de N sobre o crescimento de mudas de erva-mate (Ilex paraguariensis St.Hil.) foi estudada, em casa de vegetação. Todas as mudas receberam uma fertilização base de 10 mg N.kg-1 de solo na forma de NH4NO3, 60 mg P2O5.kg-1 e 40 mg K2O.kg-1 de solo na forma de KH2PO4 quinze dias antes da aplicação dos tratamentos. Os tratamentos foram os seguintes: Controle, sem adição extra de N; Uréia = 100 mg N.kg-1 de solo como Uréia; NO3- = 100 mg N.kg-1 de solo como Ca(NO3)2; e NH4+ = 100 mg N.kg-1 de solo como (NH4)2SO4. Concluiu-se que: 1) o aumento do conteúdo de N nas folhas, por si, não é capaz de promover ganhos na produção de biomassa em mudas de erva-mate; 2) mudas que receberam N-NH4 apresentaram maior acumulo de P e Mg na biomassa aérea; e 3) o aumento na absorção de P e Mg coincidiu com um aumento na área foliar, no número de folhas e na fotossíntese liquida na fonte N-NH4.

The influence of different N sources on the growth of maté (Ilex paragurariensis St.Hil.) seedlings grown in greenhouse was studied. All seedlings received a base fertilization of 10 mg N.kg-1 soil as NH4NO3, 60 mg P2O5.and 40 mg K2O.kg-1 soil as KH2PO4 15 days before treatments application. Treatments were as follow: Control, with no extra N added; Urea = 100 mg N.kg-1 soil as Urea; NO3- = 100 mg N.kg-1 soil as Ca(NO3)2; and NH4+ = 100 mg N.kg-1 soil as (NH4)2SO4. It was concluded that: 1) increasing N content in leaves alone was not able to promote gain in biomass production of maté seedlings; 2) seedlings receiving N-NH4 showed a higher accumulation of P and Mg on shoot biomass; and 3) an increase in leaf area, leaf number and net photosynthesis observed at the N-NH4 treatment was coincident with an increasing absorption of P and Mg.

Cedrela fissilis foi semeada e mantida em dois ambientes de luz: na margem leste (tratamento sol - razão vermelho: vermelho extremo da radiação solar direta foi 1,15) e sob dossel de floresta (tratamento sombra - radiação fotossinteticamente ativa reduzida de 0.22 a 7% da radiação direta e razão vermelho:vermelho extremo de 0,21 a 0,36). O crescimento em altura e diâmetro do caule no sol foi mais rápido, o desenvolvimento do sistema radicular continua, o número de folhas foi em média o dobro das de sombra, quanto à área foliar foi 10 vezes maior em plantas de sol com 15-25 folíolos por folha, enquanto as plantas da sombra desenvolveram de 5 a 10. Nas plantas de sombra, as maiores proporções de matéria seca foram investidas em parte aérea. A capacidade de troca de gases das plantas da sombra expostas na radiação alta não aumentou. Quando plantas de sombra foram transferidas ao sol e mantidas por 15 dias, apenas as folhas jovens se adaptaram às condições de radiação aumentada, atingindo a capacidade de resposta das folhas das plantas de sol, enquanto as folhas mais velhas caíram. As plantas de sol transferidas para a sombra não perderam as folhas, mas não atingiram a resposta das plantas de sombra.

Seeds of Cedrela fissilis Vellozo were planted and maintained under two distinct conditions: at east border of a forest with red: far-red ratio of 1.15 and under canopy with photosynthetic photon flux density of 0.22-7% of full sun radiation and red: far-red ratio of 0.21-0.36. Seedling growth (height and stem diameter) was faster under sun, the development of roots more continuous and the number of leaves almost twice of that of shade plants. The leaf area was 10 times greater in sun plants with 15-25 leaflets per leaf while under shade only 5 to 10 leaflets were found per leaf. In shade plants, a higher proportion of dry mass was found in aerial parts. Leaves of sun plants had the capacity of gas exchange to respond to high light radiation, but leaves adapted to shade presented a lower response to light changes. When shade plants were transferred and maintained under the sun for 15 days, only the young leaves were adapted to increased light radiation, reaching the same photosynthetic rate as sun plants, while old leaves were shed. Sun plants transferred to shade conditions did not lose leaves, but did not reach the same photosynthetic rate attained by shade plants.