Abstract Titanium dioxide nanotubes (TiO2-nts) were incorporated into a glass ionomer cement (GIC) with improved mechanical properties and antibacterial activity. The aims of the present in vitro study were to define the elemental characterization, aluminum (Al) release rate, and initial working time for GIC reinforced with TiO2-nts, in an experimental caries model. TiO2-nts were incorporated into GIC powder components at 5% by weight, and compared with unblended GIC. Experimental approaches used energy-dispersive spectrometry (EDS), atomic absorption spectrophotometry (AAS), and brightness loss to define surface element properties, Al release rates, and initial working time, respectively. Statistical analysis was performed by 2-way ANOVA, Tukey’s test, generalized linear models, and Student’s t test (a = 0.05). EDS data analysis revealed that TiO2-nts incorporated into GIC had no significant impact on the typical elemental composition of GICs in an in vitro caries model. Regarding the demineralizing solution, GIC with TiO2-nt significantly decreased the Al release rate, compared with the control group (p < 0.0001). Moreover, TiO2-nt incorporated into GIC did not alter the initial working time of the material (p > 0.05). These findings add information to our scientific body of knowledge concerning the potential impact of TiO2-nt on the performance of conventional GICs.

Resumo O objetivo deste estudo foi avaliar o efeito do digluconato de clorexidina 2% (CHX) na resistência de união à microtração (µTBS) entre um sistema adesivo e sob 3 condições de dentina. Para tanto, este estudo avaliou a interface adesiva no início, após 6 meses e 1 ano de armazenamento. Quarenta e oito terceiros molares humanos foram preparados e divididos aleatoriamente em 3 grupos, de acordo com os substratos dentinários: dentina hígida (Dh), dentina infectada por cárie (Di) e dentina afetada por cárie (Da). Os grupos foram subdivididos em dois de acordo com o pré-tratamento: aplicação de CHX 2% ou sem pré-tratamento (controle). As superfícies da dentina foram condicionadas com gel de ácido fosfórico a 35%, em seguida, utilizou-se o sistema adesivo Adper Single Bond 2 (3M ESPE) de acordo com as instruções do fabricante. Posteriormente, os corpos-de-prova foram armazenados em água deionizada a 37°C por 24h, 6 meses e 1 ano. Dois dentes adicionais foram usados para analisar as interfaces de união no MEV. Os dados foram submetidos a ANOVA três fatores em esquema de parcela subdividida e ao teste de Tukey (α = 0,05). Os resultados mostram que a Di diminuiu os valores de µTBS quando comparado as Da e Dh, independente do tempo de armazenamento ou tratamento. Amostras armazenadas por 6 meses e 1 ano diminuíram a µTBS para o grupo de controle, mas nenhuma diferença foi encontrada entre o tempo de armazenamento para o grupo CHX. A aplicação de CHX 2% após o condicionamento apresentou melhora na resistência de união à dentina no tempo de armazenamento, independente dos substratos avaliados.

Abstract The aim of this study was to evaluate the effect of 2% chlorhexidine digluconate (CHX) on microtensile bond strength (µTBS) between an adhesive system and under 3 dentin conditions. For that, this study evaluated the adhesive interface at initial, after 6 months and 1 year of storage. Forty-eight human third molars were prepared and randomly divided into 3 groups, according to dentin substrates: sound dentin (Sd), caries-infected dentin (Ci) and caries-affected dentin (Ca). The groups were subdivided into two according to the dentin pre-treatment: application of 2% CHX or without pre-treatment (control). The dentin surfaces were etched with 35% phosphoric acid gel and bonded with Adper Single Bond 2 (3M ESPE) adhesive system according to manufacturer’s instructions. Subsequently, the specimens were stored in deionized water at 37°C for 24h, 6 months and 1 year. Two additional teeth were used to analyze the bonding interfaces by SEM. Data was submitted to three-way ANOVA in a split plot design and Tukey’s test (α = 0.05). The results showed that Ci decreased µTBS values when compared to Ca and Sd, regardless storages time or treatment. Stored samples for 6 months and 1 year decreased the µTBS for the control group, but no difference was found between storages time for the CHX group. As a conclusion, the 2% CHX application after etching showed improved dentin bond strength in the storage time, regardless of the substrates evaluated.

Abstract This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC.

RESUMO A reabsorção radicular é caracterizada pela perda de cemento e/ou dentina da raiz do dente como resultado de uma ação odontoclástica interna ou externa. Este caso relata uma reabsorção cervical precoce da raiz dos dentes decíduos numa criança de 8 anos de idade. Grande reabsorção radicular nas faces mesiais dos caninos decíduos superiores e inferiores direito e esquerdo (dentes #53, #63, #73 e #83), com envolvimento assintomático da polpa, foi detectada durante o exame radiográfico de rotina para planejamento ortodôntico. O paciente foi submetido à exodontia dos dentes comprometidos, utilizando duas técnicas distintas: convencional e alternativa. A técnica convencional, fórceps, foi escolhida para os caninos esquerdo maxilar e mandibular (dentes n.º 63 e n.º 73). E a técnica alternativa utilizando o instrumento periótomo, normalmente necessário para exodontias prévias a cirurgia de implantes dentários, foi utilizada para os caninos direito maxilar e mandibular (dentes n.º 53 e n.º 83). O instrumento periótomo demonstrou ser uma alternativa para melhorar a cirurgia pediátrica devido ao melhor comportamento e cicatrização do paciente, e à redução da duração do procedimento.

ABSTRACT Root resorption is characterized by the loss of cementum and / or dentin from the tooth root as a result of internal or external odontoclastic action. This case reports a premature cervical root resorption of deciduous teeth in an 8-year-old boy. Large root resorption on the mesial faces of the maxillary and mandibular right and left deciduous canines (teeth #53, #63, #73 and #83), with asymptomatic pulp involvement, was detected during routine radiographic examination for orthodontic planning. The patient was managed with extraction of the affected teeth using two different techniques: conventional and alternative. The conventional technique, forceps, was used for the maxillary and mandibular left canines (teeth #63 and #73). And alternative technique using the periotome device, usually required for extraction during dental implant surgery, was used for the maxillary and mandibular right canines (teeth #53 and #83). The periotome device showed to be an alternative to improve pediatric surgery due to better patient behavior and healing, and to reduced procedure length.

RESUMO O objetivo do presente estudo é apresentar uma abordagem de tratamento em um caso de cárie na primeira infância (CPI). Uma menina de 5 anos foi encaminhada à área de Odontopediatria com múltiplas perdas dentárias precoces, dificuldades mastigatórias, amamentação prolongada e baixa qualidade de vida, com diagnóstico de CPI severa. Um plano de tratamento em três fases foi implementado: 1. Controle da doença – consistindo em mudanças comportamentais nos hábitos de higiene bucal, orientação alimentar, remoção profissional do biofilme seguida de aplicação de flúor e restaurações provisórias; 2. Controle de infecção – exodontias e pulpotomia; e 3. Reabilitação funcional – restaurações diretas e semi-indiretas de resina, próteses parcial e total removíveis nos maxilares inferior e superior, respectivamente. Em conclusão, a abordagem multidisciplinar proposta resultou em um impacto positivo na nutrição e crescimento do paciente, produção da fala, comunicação, autoimagem e socialização, levando a uma melhoria da qualidade de vida.

ABSTRACT The aim of the current study is to present a treatment approach in a case of severe early childhood caries (ECC). A 5-year-old girl was referred to the Pediatric Dentistry Division with multiple premature tooth loss, masticatory difficulties, prolonged breast-feeding, and low quality of life, who was diagnosed with severe ECC. A three-phased treatment plan was implemented: 1. Disease control – consisting of behavioral changes in oral hygiene habits, diet guidance, professional biofilm removal followed by fluoride application and temporary restorations; 2. Infection control – teeth extractions and pulpotomy; and 3. Functional rehabilitation – direct and semi-indirect resin restorations, a removable partial and total prostheses in the lower and upper jaws, respectively. In conclusion, the proposed multidisciplinary approach resulted in a positive impact on the patient’s nutrition and growth, speech production, communication, self-image, and social functioning leading to an improved quality of life.

Resumo O objetivo deste estudo foi avaliar a influência do biofilme de S. mutans na rugosidade (Ra), brilho (GU), dureza superficial (KHN) e resistência à flexão (FS) de compósitos de Bulk Fill de alta viscosidade. Foram utilizados Filtek Bulk Fill (FBF), Tetric N Ceram Bulk Fill (TNC), X-tra fil Bulk Fill (XF) e Filtek Z350 (FZ). Dez discos de cada compósito foram preparados para Ra, KHN e GU e 20 barras para o FS. Após 24 h, os espécimes foram polidos e as análises iniciais realizadas. As amostras foram esterilizadas e submetidas a biodegradação por 7 dias e as análises finais foram realizadas. Amostras representativas de cada grupo foram avaliadas no Microscópio Eletrônico de Varredura. Os dados foram submetidos à ANOVA dois fatores e teste de Tukey. XF apresentou os maiores valores (p<0,05) de Ra antes e após a biodegradação (0,1251; o00,3100) e FZ (0,1443) os menores após a biodegradação (p<0,05). Os maiores valores de GU (p<0,05) foram observados para FZ (71,7; 62) e FBF (69,0; 64,6), e os mais baixos (p<0,05) para TNC (61,4; 53,3) e XF (58,5; 53,5), ambos antes e depois da biodegradação. Para KHN, os valores mais altos foram obtidos por XF (151,7; 106), e os (p<0,05) valores mais baixos para TNC (62,2; 51,8), antes e depois da biodegradação. Os maiores valores (p <0,05) de FS foram observados para a FZ (127,6) e os menores (p<0,05) para a TNC (86,9); após biodegradação, o XF (117,7) apresentou os maiores valores (p<0,05) em comparação ao TNC e FZ. Em conclusão, a biodegradação aumentou o Ra e diminuiu a GU e a KHN para todos. Em relação à FS, a degradação promoveu uma diminuição significativa apenas para a FZ.

Abstract The aim of this study was to evaluate the Streptococcus mutans biofilm influence on the roughness (Ra), gloss (GU), surface hardness (KHN) and flexural strength (FS) of high viscosity bulk fill composites. Filtek Bulk Fill (FBF), Tetric N Ceram Bulk Fill (TNC), X-tra fil Bulk Fill (XF) and Filtek Z350 (FZ) were used. Ten discs of each composite were prepared for Ra, KHN and GU and 20 bars for the FS. After 24 h, specimens were polished and initial analyzes performed. Samples were sterilized and subjected to biodegradation for 7 days and final analyzes performed. Representative samples of each group were evaluated in Scanning Electron Microscope. Data were submitted to ANOVA two factors and Tukey test. XF presented the highest values (p<0.05) of Ra before and after biodegradation (0.1251; 0.3100), and FZ (0.1443) the lowest after biodegradation (p<0.05). The highest GU values (p<0.05) were observed for FZ (71.7; 62) and FBF (69.0; 64.6), and the lowest (p<0.05) for TNC (61.4; 53.3) and XF (58.5; 53.5), both before and after biodegradation. For KHN the highest values were obtained by XF (151.7; 106), and the (p< 0.05) lowest values for TNC (62.2; 51.8), both before and after biodegradation. The highest values (p<0.05) of FS were observed for FZ (127.6) and the lowest (p<0.05) for TNC (86.9); after biodegradation, XF (117.7) presented the highest (p<0.05) values compared to TNC and FZ.” In conclusion, biodegradation increased Ra and decreased GU and KHN for all. Concerning FS, degradation provided a significant decreased value only for FZ.

Abstract The composition of infiltrants can influence their physical properties, viscosity and depth of penetration (DP). Strategies are used to increase the DP, such as the addition of diluents or the use of heat. This study aimed to evaluate the effect of preheating and composition on physical properties and DP of infiltrants in demineralized enamel. The groups were assigned, and the following experimental formulations were made: 25%BisEMA +75%TEGDMA; 25%BisEMA +65%TEGDMA +10%ethanol; 25%BisEMA +65%TEGDMA +10%HEMA; 100%TEGDMA; 90%TEGDMA +10%ethanol; 90%TEGDMA +10%HEMA. The samples were photoactivated at two temperatures (25°C and 55°C). Degree of conversion (DC) was performed using an infrared spectrophotometer. Elastic modulus (E), flexural strength (FS) and contact angle (CA) tests were also performed. The DP of an infiltrant in demineralized enamel was determined by confocal laser scanning microscopy (CLSM) using an indirect labeling technique. The data were analyzed by two-way ANOVA and Tukey’s test. DC increased after preheating in all the groups; however, 90%TEGDMA+10%ethanol showed the lowest DC for both temperatures, and the lowest E. Preheating did not influence E or FS. The CA increased at 55°C for most groups, but decreased for groups containing HEMA. Temperature did not seem to influence DP, and Icon showed the lowest DP values. The 100%TEGDMA composition showed more homogeneous penetration, whereas Icon showed heterogeneous and superficial penetration. The preheating technique does not improve all properties in all the material compositions. The composition of a material can influence and improve its properties.

Resumo O objetivo foi avaliar as alterações de brilho e cor de materiais restauradores estéticos submetidos ao armazenamento em diferentes bebidas ácidas. Foram preparados espécimes de compósitos de resina (Z350XT (Z350), IPS Empress Direct (ED), Charisma Diamond (CD) e o brilho e a cor (ΔE) de superfície mensurados (n=10). Em seguida, os espécimes foram imersos em 4 mL de cada uma das diferentes bebidas (suco cranberry, Coca-Cola, café, ou saliva artificial) durante 15 min, 3x/dia durante 14 dias e o brilho e a cor novamente mensurados. A alteração de cor foi avaliada pela fórmula ΔE e os valores de alteração de brilho foram obtidos pela fórmula: (brilho final - brilho inicial). Os dados foram submetidos à ANOVA e Tukey (a = 0,05). CD apresentou menor alteração de cor quando comparado aos demais compósitos e maiores valores de ΔE foram obtidos após imersão em suco de café e cranberry. O café promoveu maior perda de brilho, seguido pelo suco cranberry, Coca-cola e saliva artificial (p <0,05). O tipo de bebida influenciou significativamente no brilho dos compósitos resinosos. Coca-cola reduziu o brilho dos três compósitos de maneira similar. O café promoveu maior alteração do brilho do ED, quando comparado ao Z350 e CD, enquanto o suco de cranberry afetou mais o Z350 do que o ED e o CD. A saliva teve efeito mais significativo na perda do brilho do CD do que o ED. As bebidas utilizadas neste estudo influenciaram as propriedades ópticas de superfície dos compósitos.

Abstract This study evaluated gloss and color changes of esthetic restorative materials subjected to different acidic beverages. Specimens of resin composites (Z350XT (Z350), IPS Empress Direct (ED), Charisma Diamond (CD)) were prepared and the initial surface gloss and color (ΔE) were measured (n=10). Then, the specimens were immersed in 4 mL of each of the different beverages (cranberry juice; Coca-Cola; coffee or artificial saliva) during 15 min, 3x/day for 14 days and new gloss and color readings were obtained. Color change was evaluated with the ΔE formula and gloss change values were obtained by the formula: (final gloss - initial gloss). Data was submitted to two-way ANOVA followed by Tukey’s post hoc test (a=0.05). CD showed the lowest color change among resin composites. The highest ΔE values were obtained after immersion in coffee and cranberry juice. Coffee promoted the highest gloss change (worst gloss retention), followed by cranberry juice, Coca-cola and artificial saliva (p<0.05). The type of beverage significantly influenced the gloss of resin composites. Coca-cola reduced gloss of the three resin composites in a similar manner. Coffee affected the ED gloss more than that of Z350 and CD, while cranberry juice affected Z350 more than ED and CD. Saliva had a more pronounced effect on the gloss retention of CD than ED. The beverages used in this study influenced the optical surface properties of the composites studied.

Resumo Introdução Muita publicidade sobre enxaguatórios bucais é veiculada em todos os meios de comunicação apelando para o efeito anti-placa e prestando um desserviço à comunidade. Grande quantidade de enxaguatórios bucais está disponível no mercado e estes diferem em suas composições e eficácia antimicrobiana. Objetivo Neste estudo, avaliamos a atividade antimicrobiana de 35 enxaguatórios bucais amplamente disponíveis contra espécies bacterianas envolvidas na iniciação do biofilme dental - Streptococcus gordonii, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius e Streptococcus sanguinis. Material e método A Concentração Inibitória Mínima (CIM) e a Concentração Bactericida Mínima (CBM) dos enxaguatórios avaliados foram determinadas de acordo com os protocolos do Clinical & Laboratory Standards Institute. Os dados foram submetidos ao teste Kruskal-Wallis e Mann-Whitney post hoc (α=0,05). Resultado Aproximadamente 70% dos enxaguatórios bucais alcançaram alta atividade antibacteriana e 30%, baixa atividade antibacteriana contra todas as espécies testadas. O enxaguatório bucal mais ineficaz mostrou atividade antibacteriana (CIM) na diluição de 1:1, enquanto a mais eficaz mostrou atividade mesmo na diluição de 1:2048, o que pode implicar em efeito prolongado na boca. Cerca de 51% dos enxaguatórios bucais apresentaram atividade bactericida, e verificou-se que formulações contendo cloreto de cetilpiridíneo ou digluconato de clorexidina estavam associados à maior atividade. Conclusão A maior parte - mas não todos – dos enxaguatórios bucais comercialmente disponíveis são eficazes na inibição de colonizadores iniciais de superfícies dentárias in vitro.

Abstract Introduction Much advertising in mouthwash is conveyed in all media appealing to the anti-plaque effect and rendering a disservice to the community. Mouth rinses are available over-the-count and differ on their compositions and antimicrobial effectiveness. Objective In this study, we evaluated the antimicrobial activity of 35 widely available mouth rinses against bacterial species involved in initiation of dental biofilm – Streptococcus gordonii, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, and Streptococcus sanguinis. Material and method The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of the evaluated mouth rinses were determined according to the Clinical & Laboratory Standards Institute protocols. Data were submitted to Kruskal-Wallis test and Mann-Whitney post hoc (α=0.05). Result About 70% of the mouth rinses achieved high antibacterial activity and 30%, a low antibacterial activity against all the species tested. The most ineffective mouth rinse showed antibacterial activity (MIC) at 1:1 dilution, while the most effective showed activity even at 1:2048 dilution, which may imply prolonged effect in the mouth. About 51% of mouth rinses showed bactericidal activity, and it was verified that cetylpyridinium chloride or chlorhexidine digluconate containing in the formulation were associated with the highest activity. Conclusion Most - but not all - mouth rinses commercially available are effective in inhibiting in vitro initial colonizers of dental surfaces.

Abstract In a previous study, we demonstrated that the incorporation of doxycycline hyclate (DOX) into resin-modified glass ionomer cement (RMGIC) inhibited important cariogenic microorganisms, without modifying its biological and mechanical characteristics. In this study, we keep focused on the effect of that experimental material as a potential therapy for arresting residual caries by analyzing other in vitro properties and conducting a pilot clinical trial assessing the in vivo effect of DOX-containing RMGIC on residual mutans streptococci after partial carious removal in primary molars. Specimens of the groups RMGIC (control); RMGIC + 1.5% DOX; RMGIC + 3% DOX; and RMGIC + 4.5% DOX were made to evaluate the effect of DOX incorporation on surface microhardness and fluoride release of RMGIC and against biofilm of Streptococcus mutans. Clinical intervention consisted of partial caries removal comparing RMGIC and RMGIC + 4.5% DOX as lining materials. After 3 months, clinical and microbiologic evaluations were performed. Data were submitted to ANOVA/Tukey or Wilcoxon/Mann-Whitney set as α=0.05. Fluoride release and surface microhardness was not influenced by the incorporation of DOX (p>0.05). There was a significant reduction of S. mutans biofilm over the material surface with the increase of DOX concentration. After clinical trial, the remaining dentin was hard and dry. Additionally, mutans streptococci were completely eliminated after 3 months of treatment with RMGIC + 4.5% DOX. The incorporation of DOX provided better antibiofilm effect, without jeopardizing fluoride release and surface microhardness of RMGIC. This combination also improved the in vivo shortterm microbiological effect of RMGIC after partial caries removal.

Resumo O objetivo foi avaliar a influência da concentração e proporção de canforoquinona (CQ)/amina terciária (DABE) na resistência à flexão (RF), módulo flexural (MF), grau de conversão (GC), amarelamento (AM), sorpção (SA) e solubilidade em água (SL) de compósitos experimentais. Blendas acrilatas foram preparadas com diferentes concentrações e proporções de CQ/DABE em peso, como segue: (CQ/DABE%): 0,4/0,4% (C1); 0,4/0,8% (C2); 0,6/0,6% (C3); 0,6/1,2% (C4); 0,8/0,8% (C5); 0,8/1,6% (C6); 1,0/1,0% (C7); 1,0/2,0% (C8); 1,5/1,5% (C9); 1,5/3,0% (C10). Para RF e MF, espécimes retangulares (7x2x1 mm, n=10) foram fotoativados com LED de pico único (Radii Cal) por 20 s e testados em máquina Instron (0,5 mm/min). Após, o GC dos mesmos espécimes (fragmentos) foi mensurado por FTIR. Para AM, discos de compósito (5x2 mm, n=10) foram preparados, fotoativados por 20 s e imediatamente avaliados em espectrofotômetro, considerando o aspecto b do sistema CIEL*a*b*. Para SA e SL, discos de compósito (5x0,5 mm, n=5) foram preparados e seus volumes calculados (mm³). Após desidratação, as amostras foram pesadas e armazenadas em água destilada por 7 dias, pesadas e novamente desidratadas e pesadas, para se calcular o SA e SL. Os dados foram submetidos a one-way ANOVA e teste de Tukey (5%). Os grupos C8, C9 e C10 mostraram maior GC, MF e AM, comparado aos outros grupos. No entanto, RF e SA foram similares entre todos os grupos. Ainda, C1, C2 e C3 apresentaram maior SL em 7 dias comparado aos outros grupos. Em geral, maiores concentrações de CQ promoveram melhores propriedades físico-mecânicas; no entanto, levaram ao maior amarelamento dos compósitos.

Abstract The aim in this study was to evaluate the influence of different ratio of camphorquinone/tertiary amine concentration on the flexural strength (FS), elastic modulus (EM), degree of conversion (DC), yellowing (YL), water sorption (WS) and water solubility (WSL) of experimental composites. Thus, acrylate blends were prepared with different camphorquinone (CQ) and amine (DABE) concentrations and ratios by weight: (CQ/DABE%): 0.4/0.4% (C1), 0.4/0.8% (C2), 0.6/0.6% (C3), 0.6/1.2% (C4), 0.8/0.8% (C5), 0.8/1.6% (C6), 1.0/1.0% (C7), 1.0/2.0% (C8), 1.5/1.5% (C9), 1.5/3.0% (C10). For the FS and EM, rectangular specimens (7x2x1 mm, n=10) were photo-activated by single-peak LED for 20 s and tested at Instron (0.5 mm/min). Then, the same specimens were evaluated by FTIR for DC measurement. For YL, disks (5x2 mm, n=10) were prepared, light-cured for 20 s and evaluated in spectrophotometer using the b aspect of the CIEL*a*b* system. For WS and WSL, the volume of the samples was calculated (mm³). For WS and WSL, composites disks (5x0.5 mm, n=5) were prepared. After desiccation, the specimens were stored in distilled water for 7 days and again desiccated, in order to measure the WS and WSL. Data were submitted to one-way ANOVA and Tukey’s test (5%). The groups C8, C9 and C10 showed higher DC, EM and YL means, compared to other composites. Therefore, the FS and WS values were similar among all groups. Also, C1, C2 and C3 presented higher WSL in 7 days, compared to other composites. In general, higher concentrations of camphorquinone promoted higher physical-mechanical properties; however, inducing higher yellowing effect for the experimental composites

Resumo O objetivo neste estudo foi avaliar a resistência de união à microtração (RUµT) de um sistema adesivo convencional (Adper Single Bond 2 - SB) em diferentes substratos dentinários e períodos de armazenagem. Vinte e quatro blocos de dentina foram obtidos de terceiros molares hígidos e separados aleatoriamente em 3 grupos (n=8): dentina sadia (Ds), dentina afetada (Da) e dentina infectada (Di). A Da e a Di foram submetidas ao desenvolvimento biológico artificial de cárie (S. mutans). O tecido cariado amolecido foi removido usando broca esférica sob inspeção visual com a solução Caries Detector (grupo Da). Considerou-se como Di a dentina amolecida e fortemente pigmentada de vermelho e como Da, a dentina hígida e levemente pigmentada de vermelho. O sistema adesivo SB foi aplicado de acordo com as recomendações do fabricante e blocos da resina composta Z350 foram construídos (6 mm de altura). O conjunto (dente/bloco de resina) foi armazenado em água deionizada por 24 horas a 37 °C. Estes foram seccionados em palitos (1,0 mm2 de área), que foram separados aleatoriamente em 3 períodos de armazenagem: 24 horas, 6 meses e 1 ano. Os palitos foram submetidos ao ensaio de resistência de união à microtração na máquina EZ teste a uma velocidade de 1,0 mm/min. Dados de RUµT foram submetidos à Análise de Variância 2 fatores em esquema de parcela subdividida e ao teste de Tukey (a=0,05). Os valores de resistência (MPa) da Ds (41,2) foram significativamente maiores do que os da Da (32,4) e Di (27,2), independente do tempo de armazenagem. Di e Da, 6 meses e 1ano, apresentaram valores similares de resistência de união. As falhas adesivas e mistas foram predominantes para todos os grupos. Em conclusão, os maiores valores de RUµT (48,1±9,1) foram verificados para a Ds e 24 h de armazenagem. A armazenagem diminuiu os valores de RUµT para todas as condições.

Abstract The aim of this study was to evaluate the μTBS in different dentin substrates and water-storage periods. Twenty-four dentin blocks obtained from sound third molars were randomly divided into 3 groups: Sound dentin (Sd), Caries-affected dentin (Ca) and Caries-infected dentin (Ci). Dentin blocks from Ca and Ci groups were subjected to artificial caries development (S. mutans biofilm). The softest carious tissue was removed using spherical drills under visual inspection with Caries Detector solution (Ca group). It was considered as Ci (softer and deeply red stained dentin) and Ca (harder and slightly red stained dentin). The Adper Single Bond 2 adhesive system was applied and Z350 composite blocks were built in all groups. Teeth were stored in deionized water for 24 h at 37 ºC and sectioned into beams (1.0 mm2 section area). The beams from each tooth were randomly divided into three storages periods: 24 h, 6 months or 1 year. Specimens were submitted to µTBS using EZ test machine at a crosshead speed of 1.0 mm/min. Failure mode was examined by SEM. Data from µTBS were submitted to split plot two-way ANOVA and Tukey’s HSD tests (a=0.05). The µTBS (MPa) of Sd (41.2) was significantly higher than Ca (32.4) and Ci (27.2), regardless of storage. Ca and Ci after 6 months and 1 year, presented similar µTBS. Mixed and adhesive failures predominated in all groups. The highest µTBS values (48.1±9.1) were found for Sd at 24 h storage. Storage of specimens decreased the µTBS values for all conditions.

Abstract The quality of the dentin root is the most important factor for restoration resin sealing and drives the outcome of endodontic treatment. Objective This study evaluated the effect of different filling pastes and cleaning agents on the root dentin of primary teeth using Fourier-transformed Raman spectroscopy (FT-Raman), micro energy-dispersive X-ray fluorescence (µ-EDXRF) and scanning electron microscopic (SEM) analysis. Material and Methods Eighty roots of primary teeth were endodontically prepared and distributed into 4 groups and filled according to the following filling pastes: Control-no filling (CP), Calen®+zinc oxide (CZ), Calcipex II® (CII), Vitapex® (V). After seven days, filling paste groups were distributed to 4 subgroups according to cleaning agents (n=5): Control-no cleaning (C), Ethanol (E), Tergenform® (T), 35% Phosphoric acid (PA). Then, the roots were sectioned and the dentin root sections were internally evaluated by FT-Raman, µ-EDXRF and SEM. Data was submitted to two-way ANOVA and Tukey tests (α=0.05). Results Regarding filling pastes, there was no significant difference in organic content. CP provided the lowest calcium values and, calcium/phosphoric ratio (Ca/P), and the highest phosphoric values. For cleaning agents there was no difference in organic content when compared to the C; however, T showed significantly higher calcium and Ca/P than PA. All groups showed similar results for phosphorus. The dentin smear layer was present after use of the cleaning agents, except PA. Conclusion The filling pastes changed the inorganic content, however they did not change the organic content. Cleaning agents did not alter the inorganic and organic content. PA cleaned and opened dentin tubules.

<sec><title>Aim:</title><p> The aim of this in vitro study was to investigate the synergistic effect of chemical degradation (erosion) and three-body abrasion (mechanical degradation) on the surface roughness (Ra) and hardness (KHN) of two nanorestorative materials and two conventional materials.</p></sec><sec><title>Methods:</title><p> Disc-shaped specimens (5 mm in diameter, 2 mm thick) of Filtek Z350^TM and TPH Spectrum^TM composites and Ketac Nano^TM and Vitremer^TM light-curing glass ionomer cements, nanomaterials and conventional materials were prepared according to the manufacturer's instructions. After 24 h, polishing procedures were performed and initial measurements of Ra and KHN were taken in all specimens. The specimens were divided into 12 groups (n = 10) according to material and storage media: artificial saliva, orange juice, and Coca-Cola(r). After 30 days of storage, the specimens were submitted to mechanical degradation and re-evaluated for Ra and KHN. Data were tested for significant differences by repeated-measure three-way ANOVA and Tukey's tests (p<0.05).</p></sec><sec><title>Results:</title><p> Erosion and abrasion wear significantly decreased hardness of all materials. Only Filtek Z350 roughness, however, was not affected by erosion and abrasion. All materials showed a significant increase in surface roughness after erosion and abrasion, except for Filtek Z350. After chemical and mechanical degradation, the KHN of all samples had decreased significantly. After mechanical degradation, the acidic drinks (Coca-Cola(r) and orange juice) were more aggressive than artificial saliva to all materials.</p></sec><sec><title>Conclusions:</title><p> A synergistic effect was observed by the increase in roughness for all materials, except for Filtek Z350; hardness values decrease for all materials, regardless of whether they were nanofilled or not. The RMGICs were more susceptible to degradation than the composites, considering both hardness and roughness surface parameters.</p></sec>

A integração interdisciplinar entre odontologia e fonoaudiologia pode proporcionar tratamento adequado das alterações dentárias e miofuncionais. Este relato de caso clínico apresenta o tratamento odontológico em criança com três anos de idade com cárie precoce da infância leve, com consequente perda dos incisivos centrais superiores devido a trauma, a reabilitação estética e funcional e tratamento fonoaudiológico. Os procedimentos clínicos odontológicos foram instrução de higiene bucal, aconselhamento dietético e realização das restaurações com resina composta devido ao acometimento por lesão cariosa dos dentes 64, 84, 85, 74, 75 (oclusal); e dentes 51, 61, 52 e 62 (face vestibular). Após um ano de preservação foi realizada a exodontia dos dentes 51 e 61 (com história de trauma anterior à primeira consulta), devido a reabsorção externa avançada. Em seguida, mantenedor de espaço estético-funcional removível foi colocado na região anterior superior. Avaliação fonoaudiológica foi realizada utilizando o protocolo Miofuncional Orofacial (MBGR), sendo verificadas as funções orofaciais, mobilidade e tônus muscular. Foram atribuídos escores para cada item avaliado no protocolo. Confirmou-se dificuldade de mobilidade dos lábios e língua com diminuição do tônus da bochecha e alterações na fala. A terapia fonoaudiológica foi estabelecida durante três meses com periodicidade semanal, havendo melhora em todos os aspectos alterados, confirmados pela adequação dos escores do Protocolo MBGR. No tratamento odontológico foram observados resultados clinicamente satisfatórios para a criança e responsáveis. Concluiu-se que o trabalho interdisciplinar entre a Odontologia e Fonoaudiologia proporcionou tratamento adequado para as condições bucais apresentadas pela criança, proporcionando saúde bucal e prognóstico favorável.

The integration of interdisciplinary dental and speech therapy can provide adequate treatment of dental and myofunctional alterations. In this case report, the dental treatment of three-year-old children with early childhood caries, premature loss of primary maxillary incisors due trauma, esthetic and functional rehabilitation and myofunctional therapy is presented. The dental care procedures included oral hygiene instructions, dietary recommendations and resin composite restorations of teeth 64, 84, 85, 74 and 75 (occlusal surface) and 51, 61, 52 and 62 (smooth surface) which were affected by dental caries. After one year of follow-up, teeth 51 and 61 were extracted (dental trauma history reported in first appointment), because the teeth presented extensive external reabsorption. Subsequently, esthetic and functional space maintainers were placed in the maxillary anterior region. Speech evaluation was performed using the MBGR protocol (orofacial myofunctional), in which the orofacial functions, mobility and muscular tone were analyzed. Scores were attributed to each item available in the protocol. Thus, the difficulty of lip and tongue movements were confirmed, in addition to reduced cheek tonus and alterations in speech. Myofunctional therapy was instituted for three months, once a week and this led to improvement in all aspects, with changes being confirmed by adequate scores obtained in application of the MBRG protocol. After dental treatment, clinical results satisfactory to both children and parents were observed. It was concluded that interdisciplinary approach involving dentistry and myofunctional therapy provided adequate treatment for oral conditions presented by children, providing oral health and favorable prognosis.