Abstract In this work, evaluated the antifungal chemosensitizing effect of the Lippia origanoides essential oil (EO) through the induction of oxidative stress. The EO was obtained by hydrodistillation and analyzed by GC-MS. To evaluate the antifungal chemosensitizing effect through induction of oxidative stress, cultures of the model yeast Saccharomyces cerevisiae ∆ycf1 were exposed to sub-inhibitory concentrations of the EO, and the expression of genes known, due be overexpressed in response to oxidative and mutagenic stress was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) method. Carvacrol and thymol were identified as the main components. The EO was effective in preventing or reducing the growth of the microorganisms tested. The gene expression profiles showed that EO promoted changes in the patterns of expression of genes involved in oxidative and mutagenic stress resistance. The combined use of the L. origanoides EO with fluconazole has been tested on Candida yeasts and the strategy resulted in a synergistic enhancement of the antifungal action of the azolic chemical product. Indeed, in association with EO, the fluconazole MICs dropped. Thus, the combinatorial use of L. origanoides EO as a chemosensitizer agent should contribute to enhancing the efficiency of conventional antifungal drugs, reducing their negative side effects. work (EO GCMS. GCMS GC MS. MS GC-MS ycf1 ycf ∆ycf subinhibitory sub inhibitory known realtime real time qRTPCR qRT PCR (qRT-PCR method components resistance L product Indeed dropped Thus drugs effects

Foi analisado o efeito de cinco lâminas de irrigação na produção do orégano, planta rica em óleo essencial, muito usada como tempero na preparação de alimentos. As lâminas de água foram baseadas na evaporação do tanque classe A (ECA) sendo: 0 ECA (sem reposição de água); 25 ECA; 50 ECA; 75 ECA e 100% ECA. As variáveis analisadas foram matéria fresca e seca de folhas e raízes, produtividade, receita líquida e eficiência do uso da água. O experimento foi instalado em Presidente Prudente (SP), de fevereiro a maio de 2006. Os melhores resultados para todas as variáveis estudadas foram encontrados com a reposição de 100% ECA. Observou-se uma relação linear positiva entre o incremento das lâminas de irrigação e as massas fresca e seca tanto da parte aérea, quanto das raízes. A aplicação da lâmina de irrigação equivalente a 100% ECA resultou na máxima produtividade observada (8.089,7 kg ha-1) e na receita líquida anual mais alta (R$ 57.637,87 ha-1).

The purpose of this experiment was to analyze the effect of five irrigation water depths on oregano production. Oregano is a plant rich in essential oil, commonly used as seasoning in food preparation. The water depths were estimated based on the class A pan evaporation (CPE): 0 CPE (without water replacement); 25 CPE, 50 CPE, 75 CPE, and 100% CPE. The analyzed variables were fresh and dry weight of leaves and roots, yield, net return, and water use efficiency. The experiment was carried out in Presidente Prudente, São Paulo State, Brazil, from February to May 2006. The best results for all the studied variables were found when 100% CPE was used. A linear positive relation was observed between water increments and fresh and dry weight of both the aboveground part and roots. The use of a water depth corresponding to 100% CPE resulted in the maximum productivity (8,089.7 kg ha-1), and in the highest net annual return (R$ 57,637.87 ha-1).