ABSTRACT Cryptococcosis is a systemic mycosis caused by the genus Cryptococcus that affects humans and animals. The present study aimed to describe the epidemiological and anatomopathological profiles, occurrence, and distribution of the infection by Cryptococcus spp. in 30 dogs and 110 cats residing in the State of São Paulo, from 2014 to 2020. The study evaluated the results from 140 necropsies and histopathology and cytopathology tests provided by Veterinary Pathology services. A higher occurrence of positive diagnosis was observed in females of both cats and dogs, adults, and mixed breeds of both species. The main diagnostic method used was histopathology, with a predominance of integumentary lesions in dogs and cats (skin pattern), with no significant difference between species and lesion location (p≤ 0.827). The time-spatial distribution showed a significant increase in the diagnoses in dogs and cats over the seven years, the identification of areas with a higher rate of diagnosis of cryptococcosis such as Campinas and São José do Rio Preto, and the positive correlation of a diagnosis with climatic data. Retrospective studies of anatomopathological data help to identify suspected cases of cryptococcosis and demonstrate the importance of monitoring and surveillance of cryptococcosis. animals profiles spp 3 11 Paulo 201 2020 14 services adults skin pattern, pattern , pattern) p≤ p (p 0.827. 0827 0.827 . 0 827 0.827) timespatial time spatial years Preto 1 20 202 082 0.82 82 2 08 0.8 8 0.

RESUMO A criptococose é uma micose sistêmica causada pelo gênero Cryptococcus, que acomete humanos e animais. O presente trabalho teve por objetivo descrever o perfil epidemiológico e anatomopatológico, a ocorrência e a distribuição da infecção em 30 cães e 110 gatos residentes no estado de São Paulo, no período de 2014 a 2020. O estudo avaliou 140 registros de necropsia e de exames histopatológicos e citopatológicos fornecidos por serviços de patologia veterinária. Os resultados apontaram maior positividade em fêmeas felinas e caninas, adultos e sem raça definida. O principal método de diagnóstico empregado foi o histopatológico, com predomínio de lesões tegumentares em cães e gatos (padrão cutâneo), não havendo diferença significativa entre espécie e localização da lesão (P≤ 0,827). A distribuição tempo-espacial demonstrou o aumento significativo de diagnósticos positivos em cães e gatos ao longo dos setes anos, a identificação de áreas com maior número de diagnósticos positivos, como Campinas e São José do Rio Preto, e a correlação positiva com os dados climáticos dos municípios. Estudos retrospectivos de dados anatomopatológicos auxiliam na identificação de casos suspeitos e demonstram a importância do monitoramento e da vigilância da criptococose. Cryptococcus animais anatomopatológico 3 11 Paulo 201 2020 14 veterinária caninas definida histopatológico padrão cutâneo, cutâneo , cutâneo) P≤ P (P 0,827. 0827 0,827 . 0 827 0,827) tempoespacial tempo espacial anos Preto municípios 1 20 202 082 0,82 82 2 08 0,8 8 0,

RESUMO O proteinograma do colostro/leite pode ser útil para diagnóstico precoce de infecções intramamárias, assim como para a avaliação da intensidade da resposta inflamatória. Este estudo teve como objetivo avaliar o perfil de proteínas, imunoglobulinas, lactoferrina e gamaglutamiltransferase no colostro de cabras leiteiras portadoras de infecções intramamárias. Os animais foram distribuídos em dois grupos, GI (n = 12), composto por cabras sem isolamento microbiológico das glândulas mamárias, e GII (n = 8), composto por cabras com resultados positivos na cultura do leite de pelo menos uma das glândulas mamárias. O diagnóstico de infecção intramamária foi realizado logo após o parto, por meio de isolamento microbiológico e de contagem de células somáticas (CCS). Os níveis de proteína total, imunoglobulina A (IgA), imunoglobulina (G) (IgG), lactoferrina, albumina e atividade da gamaglutamiltransferase (GGT) foram avaliados em amostras de colostro/leite de transição, usando-se SDS-PAGE logo após o parto bem como às 24 e às 48 horas após esse evento. Staphylococcus não aureus (NAS) foram encontrados em todos os isolamentos. A concentração de IgG foi superior no GII apenas às 48h, ao mesmo tempo em que o CCS foi de 1660,25 x 10³/mL. No entanto, a proteína total foi maior neste grupo às 24 e às 48h. A albumina foi maior nas cabras com mastite às 24h, e a lactoferrina no momento 0h (M0) e às 48h. Em geral, os valores de IgG, lactoferrina e albumina diferiram entre os animais com e sem infecções intramamárias no M0. A atividade da gamaglutamiltransferase não foi influenciada pela infecção intramamária. Os resultados deste estudo reforçam a importância do proteinograma como uma ferramenta auxiliar no diagnóstico da mastite em cabras leiteiras.

ABSTRACT Proteiongram analysis is useful for the early diagnosis of intramammary infections during the period of colostrogenesis. This study aimed to evaluate the profile of total proteins, immunoglobulins, lactoferrin, and gamma-glutamyl transferase (GGT) in the colostrum of dairy goats with intramammary infections. Animals were divided in groups: GI (n=12) of goats without mammary gland infections, and GII (n=8) of goats with mammary gland infections. Intramammary infections were diagnosed using microbiological isolations and somatic cell counts (SCCs). Total protein was evaluated in the samples using SDS-PAGE shortly after parturition, and 24 and 48 hours after that event. Non-aureus Staphylococcus (NAS) were detected in all isolates. At 48 h, GII had high IgG levels and a SCC of 1660.25 × 10³/mL. Levels of total protein were high in this group at 24 and 48 h. Albumin levels were high in goats with mastitis at 24 h. Overall, the IgG, lactoferrin, and albumin levels differed between animals with and without intramammary infections at M0. GGT activity was not influenced by the intramammary infection. The results of this study reinforce the importance of the proteinogram as an auxiliary tool in the diagnosis of mastitis in dairy goats.

O objetivo deste trabalho foi avaliar a transferência de imunidade passiva de cabras, que pariram com mastite, para seus respectivos cabritos. Os animais foram distribuídos em dois grupos, a saber: grupo 1 (GI), constituído por cabritos, filhos de cabras sem isolamento microbiológico em ambas as glândulas mamárias, e grupo 2 (GII), composto por cabritos, filhos de cabras com resultado positivo à lactocultura, em pelo menos uma das glândulas mamárias. Foram coletadas amostras de colostro e sangue à parição, bem como às 24 e às 48 horas após o parto/nascimento. O diagnóstico e o monitoramento da mastite nos animais foram realizados por meio do California Mastitis Test (CMT), contagem de células somáticas e isolamento microbiológico. A proteína total foi mensurada pelo método do biureto, e as concentrações de imunoglobulina A (IgA), imunoglobulina G (IgG), transferrina, albumina e haptoglobina por meio da eletrofoerese em gel de poliacrilamida contendo dodecil sulfato de sódio (SDS-PAGE). Os agentes mais isolados na cultura microbiológica foram os Staphylococcus coagulase negativa. Não houve diferença significativa (P<0,05) entre os valores médios de imunoglobulina G (IgG) nos cabritos provenientes de cabras com mastite quando comparados aos recém-nascidos oriundos de cabras livres de infecções intramamárias. Da mesma forma, a atividade de gamaglutamiltransferase (GGT) não mostrou diferença entre os grupos em todos os momentos avaliados. A ingestão de colostro decorrente de cabras com mastite não causou falha na transferência de imunidade passiva nos respectivos conceptos.

The aim of this study was to evaluate the transfer of passive immunity goats kidded with mastitis to their kids. The animals were divided into two groups, namely: Group 1 (GI) containing kids, sons of goats without microbiological isolation in both mammary glands, and Group 2 (GII), composed of kids, sons of goats with positive result to lactoculture in at least one of mammary glands. Colostrum samples and blood were collected after delivery, 24 and 48 hours after delivery / birth. The diagnosis and monitoring of mastitis in animals were performed using the California Mastitis Test (CMT), somatic cell count and microbiological isolation. Total protein was measured by the biuret method, and the concentrations of immunoglobulin A (IgA), immunoglobulin G (IgG), transferrin, albumin and haptoglobin through eletrofoerese polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE). The agents most isolated in microbiological culture were coagulase-negative Staphylococcus. There was no significant difference (p <0.05) between the acquisition of immunoglobulin G (IgG) in goats from goats with mastitis compared to infants originating free goat mammary infections. Similarly the gamma glutamyl transferase (GGT) was equal in the comparison between groups in all evaluated moments. The colostrum intake resulting from goats with mastitis caused no failure in the passive transfer of immunity in their fetuses.

Os pombos possuem uma rica diversidade de microrganismo, entre eles fungos sapróbios, como do gênero Cryptococcus, que podem atuar como agentes patogênicos para o homem e animais. Objetivou-se o isolamento, a caracterização bioquímica e a molecular de amostras de Cryptococcus spp. de excretas ambientas de pombos. Foram colhidas 100 amostras ambientais de pontos equidistantes e representativos da área da cidade de Araçatuba, São Paulo. As amostras foram rasteladas do solo de vias públicas, armazenadas em frasco coletor e encaminhadas para o Laboratório de Bacteriologia e Micologia da FMVA, onde foram processadas e cultivadas em duplicata, em placas de Petri contendo ágar Sabouraud dextrose a 4% e Niger. Em seguida, foram incubadas à temperatura ambiente e a 30ºC, respectivamente, por um período não inferior a 15 dias. Após a observação diária, as colônias sugestivas para levedura foram reisoladas em ágar Niger e submetidas a testes bioquímicos para posterior caracterização molecular pela técnica da PCR. Como resultado, a caracterização bioquímica e a molecular isolaram 32 colônias leveduriformes, sendo 8% dos cultivos positivos para Cryptococcus neoformans var. neoformans, 17% para Rhodotorula rubidae e 7% Candida albicans. Pelo exposto, concluiu-se que excretas ambientais de pombos constituem um microfoco para Cryptococcus neoformans var. neoformans e outras leveduras com potencial patogênico, representando um risco à saúde pública, sendo necessárias medidas preventivas, como a higienização com a correta remoção das excretas, a fim de minimizar os riscos de exposição ambiental.

ABSTRACT Pigeons have a rich diversity of microrganisms, including fungi saprobes such as the genus Cryptococcus that can act as a pathogen for humans and animals. The aim of this was their isolation, biochemical and molecular characterization of samples of Cryptococcus from ambientas avian excreta. One hundred environmental samples,representative of the area equidistant from Araçatuba, São Paulo points were collected. The samples were removed and stored in collection bottles, sent to the Laboratory of Bacteriology and Mycology of FMVA, where they were processed and cultivated in duplicate on Petri dishes containing Sabouraud dextrose 4% and Niger and incubated at room temperature 30° C, respectively, for a period of no less than 15 days. Colonies after daily observation which were suggestive for yeast growth were re-isolated in Niger agar and subjected to biochemical analisis for further molecular characterization by PCR tests. As results, the biochemical and molecular characterization of 32 yeast colonies revealed 8% of cultures positive for Cryptococcus neoformans var. neoformans, 17% Rhodotorula rubidae and 7% Candida albicans. It was concluded that environmental avian excreta are active sites for replication of Cryptococcus neoformans var. neoformans and other yeasts with pathogenic potential. These constitute a public health risk, and preventive measures are necessary, such as cleaning and complete removal of excreta in order to minimize the risk of environmental exposure.

O gênero Cryptococcus caracteriza-se por ser uma levedura responsável por infecção sistêmica, causada pelas espécies Cryptococcus neoformans e Cryptococcus gattii. O fungo é encontrado em substratos de origem animal e vegetal, e a infecção ocorre com a inalação de basidiósporos ou leveduras desidratadas infectantes presentes no ambiente. O presente trabalho teve por objetivo pesquisar a existência de microfocos de Cryptococcussp.em amostras ambientais da cidade de Araçatuba, São Paulo, com a finalidade de minimizar os riscos de contaminação do homem e dos animais, buscando o conhecimento da ecoepidemiologia do Cryptococcus. Foram colhidas 50 amostras oriundas de ocos e troncos de árvores (Cassiasp., Ficussp., Caesalpinea peltophorides) de 10 locais representativos do perímetro urbano, as quais foram encaminhadas ao Laboratório de Bacteriologia e Micologia da Faculdade de Medicina Veterinária de Araçatuba-Unesp, onde foram processadas e semeadas em placas de Petri contendo ágar semente de Níger e Sabouraud dextrose com clorafenicol e incubadas à temperatura de 30ºC, por um período não inferior a cinco dias. Posteriormente, foram submetidas às provas bioquímicas: produção de urease, termotolerância a 37ºC e quimiotipagem em ágar CGB (L-canavanina-glicina-azul de bromotimol). A análise dos resultados revelaram que 17 (34%) dos cultivos foram positivos para o gênero Cryptococcus, sendo nove (18%) para Cryptococcus gattiie oito (16%) para Cryptococcus neoformans. Outras leveduras correlacionadas, como Rhodotorula sp. e Candida sp., também foram isoladas. Conclui-se que os basidiósporos de Cryptococcusencontram-se dispersos na natureza, constituindo microfocos ambientais, não vinculados necessariamente a um único hospedeiro.

Cryptococcosis is an opportunistic fungal infection caused by Cryptococcusyeasts, especially C. neoformans and Cryptococcus gattii. The fungus is found in substrates of animal and vegetable origin, and infection occurs through inhalation and seedlings present in the environment. The present study aimed to investigate the existence of microfocus Cryptococcus sp. from the environmental samples of Araçatuba city, São Paulo, featuring new niches, by decoupling the direct relationship between fungus and host in order to minimize the risk of contamination of man and animals, understanding the ecoepidemiology of Cryptococcus. Fifty samples from hollows and tree trunks were harvested (Cassia sp., Ficus sp., Caesalpinea peltophorides) from ten representatives in the urban perimeter. The samples were immediately sent to the Laboratory of Bacteriology and Mycology, Faculty of Veterinary Medicine Araçatuba - Unesp where they were processed and plated on Petri dishes containing agar seed Niger and Sabouraud dextrose agar with chloramphenicol, incubated at 30ºC for a period of no less than 5 days. Afterwards they were subimitted to biochemical tests: urease production, thermotolerance at 37°C and quimiotipagem in CGB agar (L- Canavanine-Glycine-Bromothymol blue). The results showed that 17 (34%) cultures were positive for Cryptococcus, 9 (18%) for Cryptococcus gattii and 8 (16%) for Cryptococcus neoformans. Other yeast correlated as Rhodotorula sp. and Candida sp. were isolated. We conclude that the infectious propagules of Cryptococcus are dispersed in nature and constitute an environmental microfocus, not necessarily being bound to a single host.

Linfomas não-Hodgkin (LNH) extranodais representam cerca de um terço de todos os linfomas e atualmente apresentam taxa de incidência maior que a de linfomas nodais. Diferenças entre LNH nodais e extranodais incluem etiologia, formas de apresentação e resposta terapêutica, entretanto não dispomos de dados na nossa população. Este estudo teve como objetivo caracterizar os LNH extranodais diagnosticados no Hospital Aristides Maltez, em Salvador-Bahia. Foram avaliados, retrospectivamente, 145 diagnósticos de linfoma não-Hodgkin, segundo a OMS-2001, no período de janeiro de 1999 a julho de 2001. A freqüência de linfomas extranodais foi de 30,3%. A idade média dos pacientes foi de 55,6 anos e a relação homem/mulher foi de 1:1. A maioria dos pacientes apresentava estadios avançados (III ou IV de Ann Arbor), presença de sintomas B, LDH normal, bom desempenho pela escala do ECOG e IPI entre zero e dois. Nove pacientes estão vivos e em remissão completa (22,5%) após um seguimento médio de 23 meses. O sítio extranodal mais comumente acometido foram as tonsilas, seguidas pela cavidade oral, pele e trato gastrointestinal, dentre outros. O linfoma difuso de grandes células B foi o mais comum subtipo histológico, seguido pelo linfoma anaplásico de grandes células. Concluímos que o mais freqüente sítio extranodal de apresentação em nosso estudo difere da maioria da literatura, porém nossa freqüência de linfoma extranodal é semelhante à mesma.

Extranodal non-Hodgkins lymphomas represent approximately one third of all lymphomas and currently have an incidence higher than nodal lymphomas. Differences in etiology, presentation and outcome of these lymphomas have been reported. However, there are no data in our population. This study was carried out in the Pathological Anatomy Service of Aristides Maltez Hospital in Salvador, Bahia. One hundred and forty-five non-Hodgkins lymphomas cases according to the WHO-2001 classification detected between January 1999 and July 2001 were evaluated. The frequency of extranodal lymphomas was 30.3%. The mean age of the patients was 55.6% years and the male/female ratio was 1:1. The majority of the patients presented with advanced stages, B symptoms, normal LDH, ECOG between o and 2 and IPI between O and 2. Nine patients are still alive in complete remission (22.5%) with a mean follow-up of 23 months. The main extranodal sites were the tonsils followed by the oral cavity, skin and gastrointestinal tract. Diffuse large B-Cell lymphoma was the mains histological subtype, followed by anaplastic large-cell lymphoma. In summary, the mains extranodal site in our study was different from the masority of reports. However our extranodal lymphoma frequency was similar.

Leptospirosis is a public health problem worldwide and its etiology remains unclear. Its pathogenesis involves a complex interaction between host and infecting microorganism. The inflammatory reaction that controls the infection process also underscores many pathophysiological events occurring in leptospirosis. We investigated the presence of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in renal tissues by immunohistochemical and histopathological examination in animals experimentally inoculated with Leptospira serovar Canicola. All the tests were carried out 2, 7, 14, 21 or 28 days after inoculation. Although TNF-α and IL-6 had been detected in tissues throughout the observation period, these cytokines appeared more intensely during the initial phase of infection. Therefore, both TNF-α and IL-6 were associated with the immunopathogenesis of leptospirosis. This profile suggests a high immunocellular response throughout the early infection stages followed by subsequent humoral response.

Leptopspirosis is a syndrome with different clinical manifestations including the most severe and often fatal forms of pulmonary disease of unknown etiology. Pulmonary injury during the inflammatory process has been associated with the excessive number of alveolar macrophages (AMs) and polymorphonuclear leukocytes stimulated in the lungs and with the production of reactive oxygen and nitrogen intermediates and other inflammatory mediators. The aim of the present work was to evaluate the cellular immune response of AMs or inflammatory cells of hamsters during leptospirosis. The activity of AMs was determined by measuring nitric oxide (NO) and protein production as well as inflammatory cell infiltration in bronchoalveolar lavage (BAL) fluid. Pulmonary activity during infection was monitored by measuring pH, pressure of oxygen (PaO2), and pressure of carbon dioxide (PaCO2) in blood samples. Cellular immune response and its role in the genesis of leptospirosis have been incriminated as the main causes of tissue and pulmonary injuries, which consequently lead to the pulmonary dysfunction in severe cases of leptospirosis. The present results show a low production of NO in both supernatant of alveolar macrophage culture and BAL. In the latter, protein production was high and constant, especially during acute infection. Total and differential cell count values were 2.5X10(6) on day 4; 7.3X10(6) on day 21; and 2.3X10(6) on day 28 after infection, with lymphocytes (84.04%) predominating over neutrophils (11.88%) and monocytes (4.07%). Arterial blood gas analysis showed pulmonary compromising along with the infectious process, as observed in parameter values (mean±SD) evidenced in the infected versus control group: PaO2 (60.47mmHg±8.7 vs. 90.09mmHg±9.18), PaCO2 (57.01mmHg±7.87 vs. 47.39mmHg±4.5) and pH (7.39±0.03 vs. 6.8±1.3). Results indicated that Leptospira infection in hamsters is a good experimental model to study leptospirosis. However, some of the immune parameters showed variations which might be associated with the animal species.

The aim of the present study was to investigate the kinetics of humoral and cellular responses during leptospirosis. We observed that the presence of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) was associated with antibody production and bacterial recovery, and the compromising of both TNF-alpha and IL-6 in the immunopathogenesis of leptospirosis during an experimental infection of BALB/c mice inoculated with Leptospira interrogans serovar Canicola was verified. Results showed higher levels of TNF-alpha and IL-6 in the initial phase of infection, in which the greatest bacterial clearance was observed. However, when the bacterial recovery was compared with the kinetics of the production of antibodies, the results revealed a kinetics proportionally inverted to antibody production. This fact may be related to some inhibitory factor which could be responsible for the selective suppression of the cellular immune response. We concluded that during leptospirosis there was a greater mobilization of the cellular immune response activity, mainly in the initial phase of the infectious process, for posterior involvement of the humoral response, and that both TNF-alpha and IL-6 could be associated with the immunopathogenesis of the disease.

Immune response to leptospirosis is mainly humorally mediated, and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. However, some aspects are still unknown. For a more detailed analysis of the cellular immune response to leptospirosis infection, trials were carried out in order to determine the hydrogen peroxide and nitric oxide (H2O2 and NO) production stimulated or not by Interferon-gamma. The participation of some specific cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha); Interferon-gamma (IFN-gamma); Interleukin-6 (IL-6); and Interleukin-4 (IL-4), in the immunopathology of this infection was also investigated. For this purpose, we analyzed the supernatant from peritoneal macrophage cell culture and the splenic cells of mice genetically selected as High (H) and Low (L) antibody producers, and inbred Balb/c mice infected with Leptospira interrogans serovar icterohaemorrhagiae. The IL-6 production varied from release peaks to inhibition in H, L, and Balb/c mice. Similar behavior was observed for IL-4, produced only by H and Balb/c mice. The three strains presented constant and elevated production of TNF-alpha until day 14, suggesting its effective participation in the initial phase of the infection. Meanwhile, all the three strains presented a constant and irregular IFN-gamma production, with release peaks between the 7th and 14th days in L mice. The H and Balb/c mice strains presented a higher tendency to Th2 response pattern, whereas L mice tended towards Th1 response.

ABSTRACT The raising of ostriches (Struthio camelus) has been implanted in Brazil as a lucrative alternative in the Brazilian livestock, attracting the interest of new farmers. The knowledge of the aerobic and anaerobic gastrointestinal microflora of these birds, besides contributing to their health, would help in the identification of pathogenic microorganisms that also infect the human being and other animals. The present study was aimed to identify of the aerobioc and anaerobioc microorganisms that compose the gastrointestinal microbiota of ostriches submitted to necropsy in the Avian Pathology Service, Medicine Veterinary Course, UNESP, Campus of Araçatuba. Out of 30 birds necropsied, from both sexes and with age varying between 1 day to 3 years of age, 53 samples of Escherichia coli (61.0%), 7 of Candida spp. (8.0%), 6 of Salmonella spp. (6.9%), 5 of Morganella spp. (5.7%), 4 of Klebsiella spp. (4.6%), 3 of Providencia spp. (3.45%), 2 of Pseudomonas spp. and Staphylococcus spp. (2.3%) and 1 of Citrobacter spp., Edwarsiella tarda, Enterococcus spp., Moerella spp. and Proteus mirabilis were isolated. Of the anaerobic microbiota, 19 samples of Lactobacillus spp. (38%), 15 of Clostridium spp. (30%), 5 of Fusobacterium spp. (10%) and 4 of Peptoestreptococcus spp (8%) were isolated. From the proventriculus mucosa 15 samples of Candida spp. (51.7%), 6 of Lactobacillus spp. (20.7%), 5 of Aspergillus spp. (17,24%), 2 of Penicillium spp. (7%) and 1 of Staphylococcus spp. (3.45%) were identified. From the gizzard, 21 samples of Candida spp. (58.3%), 9 of Aspergillus spp. (25%), 5 of Lactobacillus spp. (13.9%) and 1 sample of Sthaphylococcus spp. (2.8%) were isolated. Struthio camelus livestock farmers health animals Service Course UNESP Araçatuba necropsied 61.0%, 610 61.0% , 61 0 (61.0%) 8.0%, 80 8.0% 8 (8.0%) 6.9%, 69 6.9% (6.9%) 5.7%, 57 5.7% (5.7%) 4.6%, 46 4.6% (4.6%) 3.45%, 345 3.45% 45 2.3% 23 (2.3% tarda isolated 38%, 38 38% (38%) 30%, 30% (30%) 10% 10 (10% 8% (8% 51.7%, 517 51.7% 51 (51.7%) 20.7%, 207 20.7% 20 (20.7%) 17,24%, 1724 17,24% 17 24 (17,24%) 7% (7% (3.45% identified gizzard 58.3%, 583 58.3% 58 (58.3%) 25%, 25 25% (25%) 13.9% 139 13 (13.9% 2.8% 28 (2.8% 61.0 (61.0% 8.0 (8.0% 6.9 (6.9% 5.7 (5.7% 4.6 (4.6% 34 3.45 2.3 (2.3 (38% (30% (10 (8 51.7 (51.7% 20.7 (20.7% 172 17,24 (17,24% (7 (3.45 58.3 (58.3% (25% 13.9 (13.9 2.8 (2.8 61. (61.0 8. (8.0 6. (6.9 5. (5.7 4. (4.6 3.4 2. (2. (38 (30 (1 ( 51. (51.7 20. (20.7 17,2 (17,24 (3.4 58. (58.3 (25 13. (13. (61. (8. (6. (5. (4. 3. (2 (3 (51. (20. 17, (17,2 (3. (58. (13 (61 (6 (5 (4 (51 (20 (17, (58 (17

RESUMO A criação de avestruz (Struthio camelus) vem sendo implantada no país, despontando como uma alternativa lucrativa na pecuária brasileira, atraindo cada vez mais o interesse de novos criadores. A necessidade do conhecimento da microflora aeróbica e anaeróbica que compõem o trato gastrintestinal destas aves, além de contribuir para a própria sanidade destes animais, seria de identificar-se possíveis portadores de microrganismos patogênicos para o homem e os animais. O presente trabalho teve por objetivos identificar as espécies de microrganismos aeróbios e anaeróbios que compõem a microbiota gastrintestinal de animais necropsiados pela disciplina de Ornitopatologia do Departamento de Clínica, Cirurgia e Reprodução Animal. Do total de 30 aves necropsiadas, de ambos os sexos e com idade varindo entre 1 dia a 3 anos de idade foram isoladas 53 amostras de Escherichia coli (61,0%), 7 de Candida spp. (8,0%), 6 de Salmonella spp. (6,9%), 5 de Morganella spp. (5,7%), 4 de Klebisiella spp. (4,6%), 3 de Providencia spp. (3,45%), 2 de Pseudomonas spp. e Staphylococcus spp. (2,3%) e 1 de Citrobacter spp., Edwarsiella tarda, Enterococcus spp., Moerella spp. e Proteus mirabilis. Da microbiota anaeróbica, foram isolados 19 amostras de Lactobacillus sp. (38%), 15 de Clostridium spp. (30%), 5 de Fusobacterium spp. (10%) e 4 de Peptoestreptococcus spp. (8%). Da mucosa do próventrículo foram identificadas 15 amostras de Candida spp. (51,7%), 6 de Lactobacillus spp. (20,7%), 5 de Aspergillus spp. (17,24%), 2 de Penicillium spp. (7%) e 1 de Staphylococcus spp. (3,45%), enquanto que da moela foram isolados 21 amostras de Candida spp. (58,3%), 9 de Aspergillus spp. (25%), 5 de Lactobacillus spp. ( 13,9%) e 1 amostra de Sthaphylococcus spp. (2,8%). Struthio camelus país brasileira criadores identificarse se Clínica Animal necropsiadas 61,0%, 610 61,0% , 61 0 (61,0%) spp 8,0%, 80 8,0% 8 (8,0%) 6,9%, 69 6,9% (6,9%) 5,7%, 57 5,7% (5,7%) 4,6%, 46 4,6% (4,6%) 3,45%, 345 3,45% 45 (3,45%) 2,3% 23 (2,3% tarda mirabilis sp 38%, 38 38% (38%) 30%, 30% (30%) 10% 10 (10% 8%. 8% . (8%) 51,7%, 517 51,7% 51 (51,7%) 20,7%, 207 20,7% 20 (20,7%) 17,24%, 1724 17,24% 17 24 (17,24%) 7% (7% 58,3%, 583 58,3% 58 (58,3%) 25%, 25 25% (25%) 13,9% 139 13 2,8%. 28 2,8% (2,8%) 61,0 (61,0% 8,0 (8,0% 6,9 (6,9% 5,7 (5,7% 4,6 (4,6% 34 3,45 (3,45% 2,3 (2,3 (38% (30% (10 (8% 51,7 (51,7% 20,7 (20,7% 172 17,24 (17,24% (7 58,3 (58,3% (25% 13,9 2,8 (2,8% 61, (61,0 8, (8,0 6, (6,9 5, (5,7 4, (4,6 3,4 (3,45 2, (2, (38 (30 (1 (8 51, (51,7 20, (20,7 17,2 (17,24 58, (58,3 (25 13, (2,8 (61, (8, (6, (5, (4, 3, (3,4 (2 (3 (51, (20, 17, (17,2 (58, (61 (6 (5 (4 (3, (51 (20 (17, (58 (17