ABSTRACT Objective: This study aimed to evaluate occlusion development after premature loss or extraction of deciduous anterior teeth, by means of a prospective cohort study. Methods: Fifteen infants and children aged 1 to 5 years old were longitudinally assessed (with loss or extraction of deciduous anterior teeth [n = 9], and without tooth losses [n = 6]). Photographs and dental casts at the baseline and after 24 months of follow-up were performed. Dental casts were scanned, and linear measurements were made on the digitalized models (missing tooth space, arch perimeter, arch length, arch width, intercanine length and intercanine width). The t-test was used for groups comparisons (α = 0.05). Results: Individuals’ mean age at baseline was 2.93 (± 1.18) years. No statistically significant differences were observed in the missing tooth space in the group with tooth loss during the 24 months of follow-up (p > 0.05). Arch perimeter, arch length, arch width, intercanine length and intercanine width did not show differences between the groups (p > 0.05). Qualitative photographic evaluation revealed other changes in the dental arches and occlusion, such as exfoliation and eruption of deciduous teeth, eruption of permanent teeth, self-correction or establishment of malocclusion, among others. Conclusion: The results suggest that the premature loss of deciduous anterior teeth does not affect the perimeter, length and width of the dental arches; however, other alterations that lead to malocclusion could be established. Objective Methods n 9, 9 , 9] 6. 6 . 6]) 2 followup follow up performed scanned perimeter width. width) ttest t test α 0.05. 005 0.05 0 05 0.05) Results Individuals 293 93 2.9 ± ( 1.18 118 18 p selfcorrection self correction others Conclusion however established 6] 00 0.0 29 2. 1.1 11 0. 1.

RESUMO Objetivo: Este estudo teve como objetivo avaliar o desenvolvimento da oclusão após perda prematura ou extração de dentes decíduos anteriores, por meio de um estudo de coorte prospectivo. Métodos: Quinze bebês e crianças de 1 a 5 anos foram avaliados longitudinalmente (com perda ou extração de dentes anteriores decíduos [n = 9] e sem perdas dentárias [n = 6]). Foram realizadas fotografias e modelos dentais no início e após 24 meses de acompanhamento. Os modelos dentários foram escaneados e medidas lineares foram feitas nos modelos digitalizados (espaço dentário perdido, perímetro da arcada, comprimento da arcada, largura da arcada, comprimento intercaninos e largura intercaninos). O teste t foi utilizado para comparações entre grupos (α = 0,05). Resultados: A média de idade dos indivíduos no início do estudo foi de 2,93 (± 1,18) anos. Não foram observadas diferenças estatisticamente significativas no espaço dentário perdido no grupo com perda dentária durante os 24 meses de acompanhamento (p > 0,05). O perímetro da arcada, comprimento da arcada, largura da arcada, comprimento intercaninos e largura intercaninos não apresentaram diferenças entre os grupos (p> 0,05). A avaliação fotográfica qualitativa revelou alterações nas arcadas dentárias e na oclusão, como: esfoliação e erupção de dentes decíduos, erupção de dentes permanentes, autocorreção ou estabelecimento de má oclusão, entre outras. Conclusão: Os resultados sugerem que a perda prematura de dentes anteriores decíduos não afeta o perímetro, comprimento e largura das arcadas dentárias; entretanto, outras alterações que levam à má oclusão poderiam ser estabelecidas. Objetivo prospectivo Métodos n 9 6. 6 . 6]) 2 arcada intercaninos. intercaninos) α 0,05. 005 0,05 0 05 0,05) Resultados 293 93 2,9 ± ( 1,18 118 18 p p> permanentes Conclusão entretanto estabelecidas 6] 00 0,0 29 2, 1,1 11 0, 1,

Abstract This systematic review aimed to answer the focused question: “What are the benefits of subgingival periodontal therapy on blood hematological and biochemical index, biomarkers of inflammation and oxidative stress, quality of life, and periodontal pathogen counts in patients with obesity and periodontitis?”. A systematic literature search was performed in six databases: PubMed, Embase, LILACS, Web of Science, Cochrane and SCOPUS and other sources, and a manual search was conducted as well. Inclusion criteria were randomized and non-randomized clinical trials, and before-and-after studies on patients with obesity subjected to periodontal therapy. The results were synthesized qualitatively. Risk of bias within studies was assessed using RoB 2 and ROBINS-I tools. The certainty of evidence was evaluated following the GRADE approach. Three randomized controlled trials and 15 before-and-after studies were included. Randomized controlled trials were considered to have a low risk of bias, as compared to before-and-after studies assessed as having low, serious, and critical risks of bias. Non-surgical periodontal therapy plus azithromycin, chlorhexidine, and cetylpyridinium chloride reduced blood pressure and decreased serum levels of HbA1c, hsCRP, IL-1β, and TNF-α. Salivary resistin level also decreased in patients with obesity and periodontitis after therapy and chlorhexidine mouth rinse. Before-and-after data suggest an improvement in total cholesterol, LDL, triglycerides, insulin resistance, C3, GCF levels of TNF-α, chemerin, vaspin, omentin-1, visfatin, 8-OHdG, and periodontal pathogen counts after therapy. question What index stress life periodontitis. . periodontitis?” databases PubMed Embase LILACS Science sources well nonrandomized non beforeandafter before qualitatively ROBINSI ROBINS I tools approach 1 included serious Nonsurgical Non surgical azithromycin HbA1c HbAc HbA c hsCRP IL1β, IL1β ILβ IL 1β, 1β β IL-1β TNFα. TNFα TNF α. α TNF-α rinse Beforeandafter Before cholesterol LDL triglycerides resistance C3 C TNFα, α, chemerin vaspin omentin1, omentin1 omentin 1, omentin-1 visfatin 8OHdG, 8OHdG OHdG 8 OHdG, 8-OHdG periodontitis? omentin-

Abstract The aim of this systematic review was to answer the following question: “Does alendronate, a nitrogen-containing bisphosphonate, improve or impair alveolar socket healing after tooth extraction in animal models”? To this end, a systematic review of the literature was carried out in PubMed, Scopus, LILACS, Web of Science, as well as in the gray literature up to May 2023. Preclinical studies that evaluated alveolar healing after tooth extraction and the intake of sodium alendronate compared with placebo were included. Two investigators were responsible for screening the articles independently, extracting the data, and assessing their quality through the SYRCLE’s RoB tool for randomized trials in animal studies. The study selection process, study characteristics, risk of bias in studies, impact of alendronate on bone healing, and certainty of evidence were described in text and table formats. Methodological differences among the studies were restricted to the synthesis methods. The synthesis of qualitative results followed the Synthesis Without Meta-analysis (SWiM) reporting guideline. From the 19 included studies, five were considered to have low risk, three were of unclear risk, and eleven presented a high risk of bias. The studies were considered heterogeneous regarding alendronate posology, including its dosage and route of administration. Furthermore, a variety of animal species, different age ranges, diverse teeth extracted, and exposure or not to ovariectomy contributed to the lack of parity of the selected studies. Our results indicated that alendronate monotherapy negatively affects the early phase of wound healing after tooth extraction in preclinical studies, suggesting that the bone resorption process after tooth extraction in animals treated with alendronate might impair the bone healing process of the extraction socket. In conclusion, alendronate administration restrains bone resorption, thereby delaying alveolar socket healing . Future studies should be conducted to validate these findings and to better understand the effects of alendronate therapy on oral tissues. question Does nitrogencontaining nitrogen containing bisphosphonate models models” end PubMed Scopus LILACS Science 2023 independently data SYRCLEs SYRCLE s characteristics formats methods Metaanalysis Meta analysis SWiM (SWiM guideline 1 posology Furthermore species ranges extracted conclusion tissues 202 20 2

Abstract Objective: To summarize data of clinical trials that used silver diamine fluoride (SDF) to prevent and treat caries lesions and dentinal hypersensitivity. Material and Methods: Six electronic databases were searched in May 2022. The concentration of SDF, type of usage (alone/combined), dentition, anterior/posterior teeth, tooth region, dental tissue, number of the treated surfaces, the intervention environment, participants' age, frequency and duration of SDF application, purpose, and outcome were the extracted variables. The type of study, year of publication, authors, journals, and country were also investigated. Results: From 8860 articles, S3 were selected. Most were randomized (n=38), that applied 38% SDF (n=43), alone (n=44), on multiple surfaces (n=44), only in dentin (n=36), of the crown (n=46) of anterior and posterior (n=36) primary teeth (n=39). The studies were preferably carried out outside the clinic (n=3l), only in children (n=33), with reapplication of SDF (n=30), but did not inform the duration of application (n= 19). SDF was most used to treat (n=46) only caries lesions (n=50). They were published between 2001 and 2022, mainly in the Journal of Dentistry (n=10). China (n=19) and Lo E.GM (n=19) were the countries and authors that published the most, respectively. Conclusion: The silver diamine fluoride 38% alone was most used to treat caries lesions in the dentin of the crown of all primary teeth, preferably applied on multiple surfaces, requiring re application, and outside the clinic. Objective (SDF hypersensitivity Methods 2022 alone/combined, alonecombined alone/combined , combined (alone/combined) dentition anteriorposterior region tissue environment participants age purpose variables study publication journals investigated Results 886 articles S selected n=38, n38 n n=38 38 (n=38) n=43, n43 n=43 43 (n=43) n=44, n44 n=44 44 (n=44) n=36, n36 n=36 36 n=46 n46 46 (n=46 (n=36 n=39. n39 n=39 . 39 (n=39) n=3l, n3l nl n=3l 3l l (n=3l) n=33, n33 n=33 33 (n=33) n=30, n30 n=30 30 (n=30) n= (n 19. 19 19) n=50. n50 n=50 50 (n=50) 200 n=10. n10 n=10 10 (n=10) n=19 n19 (n=19 EGM E GM respectively Conclusion 202 (alone/combined 88 n3 n=3 3 (n=38 n4 n=4 4 (n=43 (n=44 (n=4 (n=3 (n=39 (n=3l (n=33 (n=30 1 n5 n=5 5 (n=50 20 n1 n=1 (n=10 (n=1 8 (n=5 2

ABSTRACT Objective: To investigate associations/correlations between bone alterations and vitamin D status in children through data mining analyses based on observational studies. Material and Methods: Searches in PubMed, Scopus, Web of Science, and Embase databases were performed to recover studies, published until October 2022, with healthy children, which investigated the vitamin D status, related or not to undesirable bone alterations linked to bone quality (bone mineral density and bone mineral content), fracture or anthropometry. Country, study design, area of expertise (medicine, nutrition, dentistry, others), bone outcome, 25-hydroxyvitamin D data (serum or intake levels), the exams for bone diagnosis, and the results were analyzed in the VantagePointTM software. Results: Of 20,583 studies, 27 were included. The USA (n=9; 33.3%) had the highest number of publications. Cross-sectional (n=11; 40.7%), case-control (n=9; 33.3%), and cohort studies (n=7; 25.9%) contemplated the medicine and nutritional areas without any study in dentistry. Studies about bone quality (n=21; 77.8%), analyzed through dual-energy X-ray absorptiometry (DXA; n=14; 51.8%), with association (n=16; 59.2%) between the low serum levels of 25-hydroxyvitamin D and undesirable bone alterations (n=14; 51.8%) were the most prevalent. Conclusion: Most studies were conducted in the medical area and showed an association between low bone quality and low levels of 25-hydroxyvitamin D, verified through DXA. Objective associationscorrelations associations correlations Methods PubMed Scopus Science 2022 content, content , content) anthropometry Country design medicine, (medicine nutrition dentistry others, others others) outcome 25hydroxyvitamin hydroxyvitamin 25 levels, levels) diagnosis software Results 20583 20 583 20,58 2 included n=9 n9 n 9 (n=9 33.3% 333 33 3 publications Crosssectional Cross sectional n=11 n11 11 (n=11 40.7%, 407 40.7% 40 7 40.7%) casecontrol case control 33.3%, n=7 n7 (n=7 25.9% 259 n=21 n21 21 (n=21 77.8%, 778 77.8% 77 8 77.8%) dualenergy dual energy Xray X ray DXA (DXA n=14 n14 14 51.8%, 518 51.8% 51 n=16 n16 16 (n=16 59.2% 592 59 (n=14 prevalent Conclusion 202 2058 58 20,5 n= (n= 33.3 n=1 n1 1 (n=1 40.7 4 25.9 n=2 n2 (n=2 77.8 51.8 5 59.2 205 20, (n 33. 40. 25. 77. 51. 59.

Abstract To assess the in vitro and in situ effect of experimental combined fluoride and calcium nanocomposite solutions on dental caries prevention. Nanocompound mesoporous silica (MS) with calcium (Ca) and sodium fluoride (NaF) - (MSCaNaF); MS with NaF (MSNaF), NaF solution (positive control), and deionized water (negative control - CG) were studied. The specimens (n=130) were submitted in vitro to a multispecies biofilm in the presence of 2% sucrose. After 24 h and 48 h, the culture medium pH, the percent of surface mineral loss (%SML), and lesion depth (ΔZ) were analyzed. In the in situ study, 10 volunteers participated in four phases of 7-days each. The products were applied on the specimens (n=240) before 20% sucrose solution drips. The polysaccharides (SEPS and IEPS), %SML and roughness (Sa) were evaluated. There was an in vitro decrease in pH values in 24h and 48h, compared to baseline. The MSCaNaF and MSNaF groups obtained lower values of %SML and ΔZ (p < 0.05) than CG and NaF after 24h and were similar to NaF after 48h (p<0.05). In situ results showed similar SEPS and IEPS among all groups after 48h. An after 7-days, the nanocomposites had similar values (p>0.05), while NaF was similar to CG (p>0.05). After 48h, the MSCaNaF and MSNaF reduced the %SML (p<0.05). After 7-days, both experimental nanocomposites were similar to NaF (p>0.05). Regarding Sa, MSCaNaF was better than NaF for both periods (p<0.05). The nanocomposites controlled the in vitro and in situ enamel demineralization, mainly in the initial periods. prevention (MS Ca (Ca (NaF (MSCaNaF) MSNaF, , (MSNaF) positive control, control) negative studied n=130 n130 n 130 (n=130 2 4 %SML, SML (%SML) (ΔZ analyzed study 1 7days days 7 each n=240 n240 240 (n=240 20 drips IEPS, IEPS) Sa (Sa evaluated baseline p 0.05 005 0 05 p<0.05. p005 p<0.05 . (p<0.05) 7days, days, p>0.05, p>0.05 (p>0.05) p>0.05. demineralization (MSCaNaF (MSNaF n=13 n13 13 (n=13 (%SML n=24 n24 (n=24 0.0 00 p00 p<0.0 (p<0.05 p>0.0 (p>0.05 n=1 n1 (n=1 n=2 n2 (n=2 0. p0 p<0. (p<0.0 p>0. (p>0.0 n= (n= p<0 (p<0. p>0 (p>0. (n p< (p<0 p> (p>0 (p< (p>

ABSTRACT Objective: To associate caregivers' sense of coherence (SOC) and untreated caries with oral health status in children covered by a dental public health system. Material and Methods: A convenience sample of caregivers/children in Angra dos Reis, Brazil, was surveyed. Caregivers' SOC was evaluated using the 13-question version questionnaire. For both caregivers/children, sociodemographic information was acquired and caries experience was evaluated by DMFT/dmft and PUFA/pufa index. Statistical associations between children's untreated carious teeth and interest variables from the caregivers were evaluated by binary logistic regression assessed by generalized linear modeling. Results: A total of 233 pairs of caregivers-children were included. Children's untreated permanent and primary carious teeth represented 34.8% and 62.2% of the caries experience, respectively and at least one PUFA/pufa scored tooth was detected in 22.7% of them. Caregivers' DMFT was 13.5±7.0, while 33.6% scored on PUFA. A total of 62.1% of them presented untreated carious lesions. Results from the univariate model, correlating children's untreated caries and caregivers' attributes showed a statistical significance for SOC values (p<0.015), untreated decayed teeth (p<0.035), self-perception of oral health (p<0.022) and oral impact on daily performance (p<0.010). The multivariate logistic first model kept the statistical significance only for the caregiver's untreated decayed teeth. Conclusion: Caregivers' SOC and untreated carious teeth could be used as indicators of dental treatment needs in their offspring. Objective (SOC system Methods caregiverschildren Reis Brazil surveyed Caregivers 13question question 13 questionnaire DMFTdmft dmft PUFApufa PUFA pufa index childrens s modeling 23 included Childrens Children 348 34 8 34.8 622 62 2 62.2 227 22 7 22.7 13570 5 0 13.5±7.0 336 33 6 33.6 621 1 62.1 lesions p<0.015, p0015 p p<0.015 , 015 (p<0.015) p<0.035, p0035 p<0.035 035 (p<0.035) selfperception self perception p<0.022 p0022 022 (p<0.022 p<0.010. p0010 p<0.010 . 010 (p<0.010) caregiver Conclusion offspring 3 34. 62. 22. 1357 13.5±7. 33. p001 p<0.01 01 (p<0.015 p003 p<0.03 03 (p<0.035 p<0.02 p002 02 (p<0.02 (p<0.010 135 13.5±7 p00 p<0.0 (p<0.01 (p<0.03 (p<0.0 13.5± p0 p<0. (p<0. 13.5 p<0 (p<0 13. p< (p< (p

Abstract This systematic review evaluated the available evidence on whether children with molar incisor hypomineralization (MIH) have more dental fear and anxiety (DFA) and dental behavior management problems (DBMPs) than those without MIH (Prospero CDR42020203851). Unrestricted searches were performed in PubMed, Scopus, Web of Science, Lilacs, BBO, Embase, Cochrane Library, APA PsycINFO, Open Grey, and Google Scholar. Observational studies evaluating DFA and/or DBMPs in patients with and without MIH were eligible. Reviews, case reports, interventional studies, and those based on questionnaires to dentists were excluded. The methodological quality assessment was based on the Newcastle-Ottawa Scale. Random-effects meta-analyses were conducted to synthesize data on DFA. The certainty of evidence was performed according to GRADE. Seven studies that evaluated a total of 3,805 patients were included. All of them presented methodological issues, mainly in the comparability domain. Most studies observed no significant difference in DFA between children with and without MIH. The meta-analysis did not show a significant effect of MIH on the standardized units for the DFA scores (SMD = 0.03; 95%CI: -0.06–0.12; p = 0.53; I2 = 0%). Synthesis including only the results for severe cases of MIH also did not show a significant effect of the condition on DFA scores (MD = 8.68; 95%CI: -8.64–26.00; p = 0.33; I2 = 93%). Two articles found DBMPs were significantly more frequent in patients with MIH. The overall certainty of evidence was very low for both outcomes assessed. The current evidence suggests no difference in DFA between children with and without MIH; DBMPs are more common in patients with MIH. This information should be viewed with caution because of the very low quality evidence obtained. (MIH (DFA (DBMPs Prospero CDR42020203851. CDR42020203851 CDR . CDR42020203851) PubMed Scopus Science Lilacs BBO Embase Library PsycINFO Grey Scholar andor or eligible Reviews reports excluded NewcastleOttawa Newcastle Ottawa Scale Randomeffects Random effects metaanalyses meta analyses GRADE 3805 3 805 3,80 included issues domain metaanalysis analysis SMD 0.03 003 0 03 95%CI 95CI CI 95 0.06–0.12 006012 06 12 -0.06–0.12 0.53 053 53 I 0%. 0% 0%) MD 8.68 868 8 68 8.64–26.00 8642600 64 26 00 -8.64–26.00 0.33 033 33 93%. 93 93% 93%) assessed obtained CDR4202020385 380 80 3,8 0.0 9 0.06–0.1 00601 1 -0.06–0.1 0.5 05 5 8.6 86 6 8.64–26.0 864260 2 -8.64–26.0 0.3 CDR420202038 38 3, 0. 0.06–0. 0060 -0.06–0. 8. 8.64–26. 86426 -8.64–26. CDR42020203 0.06–0 006 -0.06–0 8.64–26 8642 -8.64–26 CDR4202020 0.06– -0.06– 8.64–2 864 -8.64–2 CDR420202 0.06 -0.06 8.64– -8.64– CDR42020 -0.0 8.64 -8.64 CDR4202 -0. -8.6 CDR420 -0 -8. CDR42 - -8 CDR4

Abstract The aim of this study was to evaluate the prevalence of Enterococcus species in the mouth of adults with periodontal health and periodontitis. A systematic search was made in databases in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. The search for articles was conducted in Medline/PubMed, Latin American and Caribbean Health Sciences Literature Database (LILACS), Cochrane Library, Scopus, Embase, Web of Science databases and in the System of Information on Grey Literature in Europe (SINGLE) and included articles published in English up to April 25th, 2021. Observational studies in humans with and without periodontitis were evaluated to identify the prevalence of Enterococcus species. Articles that met the inclusion criteria were analyzed and classified to determine the quality rating in good, fair, and poor. A new detailed checklist for quality assessment was developed based on the information required for applicable data extraction in reviews. The study design, sample size, demographic data, periodontal clinical parameters, microbial analysis method, biological sample, prevalence of Enterococcus spp., and correlations with periodontal clinical parameters were assessed. After screening and full-text reading, 8 articles met the inclusion criteria. All selected studies showed a significantly higher prevalence of Enterococcus spp. in patients with periodontitis compared with periodontally healthy patients. Thus, the present systematic review suggests that the prevalence of Enterococcus faecalis in the mouth of periodontitis individuals is higher than that of periodontally healthy individuals. MetaAnalysis Meta Analysis PRISMA (PRISMA guidelines MedlinePubMed Medline PubMed Medline/PubMed LILACS, LILACS , (LILACS) Library Scopus Embase SINGLE (SINGLE 25th th 2021 good fair poor reviews design size method spp assessed fulltext full text reading Thus (LILACS 202 20 2

Abstract The aim of this study was to develop and analyze the psychometric properties of the Brazilian Version of Lay Person's Social Judgement about Cleft-lip Scale (B-LSojCleft-S). A standardized photograph of a 16-year-old girl was manipulated by using photo editing software, to simulate different cleft-lip situations. The cleft-free image was used as a control. The researchers structured an initial questionnaire that was evaluated by experts and a sample of the target population to evaluate the construct. After analysis by the researchers, a final version of the B-LSojCleft-S containing 14 items was generated. Each answer was awarded score from 1 to 3 points, yielding a total score of 14 to 42 points. Higher scores represented better social judgements. The B-LSojCleft-S was applied online to 103 test participants and 73 retest participants with an interval of 20 days between application. Aspects of acceptability, discriminant property, internal consistency, reliability and construct validity were evaluated. Construct validity was assessed using the Friedman test (p < 0.05). Reliability was determined using an intraclass correlation coefficient > 0.70, and internal consistency using Cronbach's alpha > 0.81. The B-LSojCleft-S showed high acceptability, strong discriminant property, excellent internal consistency and reliability, but had a floor and ceiling effect. The instrument reached valid and reliable scores and had acceptable psychometric properties to evaluate the social judgments of lay persons about different cleft lip situations in a Brazilian population. Persons Person s Cleftlip Cleft BLSojCleftS. BLSojCleftS B LSojCleft S . (B-LSojCleft-S) 16yearold yearold 16 year old software cleftlip cleftfree free control generated points 4 judgements 10 7 2 application acceptability property p 0.05. 005 0.05 0 05 0.05) 070 70 0.70 Cronbachs Cronbach 081 81 0.81 effect (B-LSojCleft-S 00 0.0 07 0.7 08 8 0.8 0.

ABSTRACT Objective: To evaluate the presence of sequelae in primary teeth and their permanent successors, as well as associations between TDI and the presence of sequelae in both teeth. Material and Methods: In this 14-year retrospective study, 2.290 records were reviewed, 192 patients who suffered dental trauma in primary teeth were followed until the eruption of successor teeth were included. Descriptive, chi-square, and regression logistic with generalized estimating equations tests were performed (p<0.05). Results: 362 primary and successor teeth were followed. Sequelae were present in 71.8% of primary teeth and 25.7% of their successors. Teeth with complicated fracture (100%), extrusion (100%) and avulsion (100%) resulted in the largest amounts of sequelae in primary teeth and intrusion (61%) on their successors. Age was associated with sequelae in permanent teeth (p<0.01). Extrusion (OR 10.06; CI 2.12-47.63) and intrusion (OR 7.51; CI 2.73-20.70) had a higher risk to cause sequelae in primary and permanent teeth, respectively. Conclusion: The type of injury involved influenced the sequelae in traumatized teeth and their successors, and the age of the child influenced the presence of sequelae in permanent teeth. Objective successors Methods 14year year 14 study 2290 2 290 2.29 reviewed 19 included Descriptive chisquare, chisquare chi square, square chi-square p<0.05. p005 p p<0.05 . 0 05 (p<0.05) Results 36 718 71 8 71.8 257 25 7 25.7 100%, 100 100% , (100% 61% 61 (61% p<0.01. p001 p<0.01 01 (p<0.01) OR 10.06 1006 10 06 2.1247.63 2124763 2.12 47.63 12 47 63 2.12-47.63 7.51 751 51 2.7320.70 2732070 2.73 20.70 73 20 70 2.73-20.70 respectively Conclusion 1 229 29 2.2 p00 p<0.0 (p<0.05 3 71. 25. (100 6 (61 (p<0.01 10.0 1247 2.1247.6 212476 212 2.1 4763 47.6 4 2.12-47.6 7.5 75 5 7320 2.7320.7 273207 273 2.7 2070 20.7 2.73-20.7 22 2. p0 p<0. (p<0.0 (10 (6 10. 124 2.1247. 21247 21 476 47. 2.12-47. 7. 732 2.7320. 27320 27 207 20. 2.73-20. p<0 (p<0. (1 ( 2.1247 2124 2.12-47 2.7320 2732 2.73-20 p< (p<0 2.124 2.12-4 2.732 2.73-2 (p< 2.12- 2.73- (p

ABSTRACT Objective: To assess the in-vitro effect of single applications of CPP-ACP pastes and different fluoridated solutions on the prevention of dental caries around orthodontic brackets. Material and Methods: Tooth/bracket sets (n=65) were immersed in artificial saliva (1h at 37ºC) and randomly subjected to single applications (100µL; 1min) of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP emulsion), CPP-ACP with fluoride (CPP-ACPF emulsion), solutions of titanium tetrafluoride (TiF4) or sodium fluoride (NaF), or no treatment (CG). Multispecies biofilm (5 x 105 CFU/mL) was formed in the presence of 2% sucrose. After 24 h, the pH and the concentration of total soluble fluoride (TSF) were analyzed by culture medium. The presence of active white spot lesions (WSL) evaluated by macroscopic examination and the percent surface mineral loss (%SML) were analyzed. Also, the topography of enamel was detected by analysis of scanning electron microscopy (SEM). The data was assessed by chi-square, Kruskal-Wallis, and Mann-Whitney tests (p < 0.05). Results: Fluoride-containing compounds led to a smaller pH reduction than did CPP-ACP and CG (p<0.05). There was difference in TSF between the groups (p<0.05), denoted as TiF4> NaF > CPP-ACPF > CPP-ACP > CG. Regarding the presence of WSL and %SML, the NaF group obtained lower values (p<0.05), while TiF4 and CPP-ACPF were similar (p>0.05). SEM demonstrated that fluoride-free groups had a larger surface dissolution. Conclusion: Fluoridated groups including solutions and CPP-ACPF were more effective than CPP-ACP in reducing enamel demineralization around orthodontic brackets after a single application. Objective invitro vitro CPPACP CPP ACP Methods Toothbracket Tooth bracket n=65 n65 n 65 (n=65 1h h 37ºC ºC 100µL µL (100µL 1min min phosphopeptideamorphous phosphopeptide amorphous emulsion, emulsion , emulsion) CPPACPF ACPF TiF (TiF4 NaF, (NaF) . (CG) 5 ( 10 CFU/mL CFUmL CFU mL 2 sucrose (TSF medium (WSL %SML SML (%SML Also SEM. (SEM) chisquare, chisquare chi square, square chi-square KruskalWallis, KruskalWallis Kruskal Wallis, Wallis Kruskal-Wallis MannWhitney Mann Whitney p 0.05. 005 0.05 0 05 0.05) Results Fluoridecontaining Fluoride containing p<0.05. p005 p<0.05 (p<0.05) p<0.05, p>0.05. p>0.05 (p>0.05) fluoridefree free dissolution Conclusion application n=6 n6 6 (n=6 (TiF (NaF (CG 1 (SEM 00 0.0 p00 p<0.0 (p<0.05 p>0.0 (p>0.05 n= (n= 0. p0 p<0. (p<0.0 p>0. (p>0.0 (n p<0 (p<0. p>0 (p>0. p< (p<0 p> (p>0 (p< (p>

RESUMO Objetivo: Avaliar in-vitro o efeito de uma aplicação única de cremes dentais de CPP-ACP e diferentes soluções fluoretadas na prevenção da cárie dentária ao redor de braquetes ortodônticos Material e Métodos: O conjunto dentes/braquetes (n=65) foi imerso em saliva artificial (1h em 37°C) randomizado e submetido a tratamento único (100µL; 1 min) de emulsão de fosfopeptídeo de caseína-fosfato de cálcio amorfo (CPP-ACP) e CPP-ACP associado ao flúor (CPP-ACPF); soluções de tetrafluoreto de titânio (TiF4) e fluoreto de sódio (NaF); e ausência de tratamento (GC). Biofilmes multiespécie (5 x 105 CFU/mL) foram formados na presença de sacarose a 2%. Após 24h, o pH e a concentração de fluoreto solúvel total (FST) foram analisados pelo meio de cultura. Foram avaliadas a presença de lesões de mancha branca (LMB), por meio da análise de macroscopia visual, e a porcentagem de perda de dureza (%PD). Também foi verificada a topografia do esmalte, usando microscopia eletrônica de varredura (MEV). Os dados foram analisados pelos testes Qui-quadrado, Kruskal-Wallis e Mann-Whitney (p < 0,05). Resultados: Os compostos contendo flúor levaram a uma redução do pH menor do que o CPP-ACP e GC (p<0,05). Houve diferença no FST entre os grupos (p <0,05), sendo TiF4> NaF > CPP-ACPF > CPP-ACP > GC. Quanto à presença de LMB e à %PD, o grupo NaF obteve os menores valores (p<0,05), enquanto TiF4 e CPP-ACPF foram semelhantes (p> 0,05). A MEV demonstrou que os grupos sem flúor tiveram uma dissolução superficial maior. Conclusão: Os grupos fluoretados, incluindo soluções e CPP-ACPF, foram mais eficazes do que o CPP-ACP sem flúor na redução da desmineralização do esmalte ao redor dos braquetes ortodônticos após uma única aplicação. Objetivo invitro in vitro CPPACP CPP ACP Métodos dentesbraquetes dentes n=65 n65 n 65 (n=65 1h h 37°C 37C C 37 100µL µL (100µL min caseínafosfato caseína fosfato (CPP-ACP CPPACPF ACPF (CPP-ACPF) TiF (TiF4 (NaF) . (GC) 5 ( 10 CFU/mL CFUmL CFU mL 2 2% 24h (FST cultura LMB, , (LMB) visual %PD. PD %PD (%PD) MEV. (MEV) Quiquadrado, Quiquadrado Qui quadrado, quadrado Qui-quadrado KruskalWallis Kruskal Wallis MannWhitney Mann Whitney p 0,05. 005 0,05 0 05 0,05) Resultados p<0,05. p005 p<0,05 (p<0,05) <0,05, <0,05 <0,05) p<0,05, p> maior Conclusão fluoretados CPPACPF, ACPF, n=6 n6 6 (n=6 3 (CPP-ACPF (TiF (NaF (GC (LMB (%PD (MEV 00 0,0 p00 p<0,0 (p<0,05 <0,0 n= (n= 0, p0 p<0, (p<0,0 <0, (n p<0 (p<0, <0 p< (p<0 (p<

Abstract This study aimed to characterize the profile of dental anxiety in pediatric patients, identifying the effect exerted by socioeconomic factors using dental data. A cross-sectional study design with a sample of 120 children aged 7–12 years old was used. Data relating to anxiety levels prior to dental care, socioeconomic aspects (family income, education level, child’s school type), and child’s dental history (previous dental appointments, previous treatment, caries experience) were collected. Additionally, participants completed the Brazilian version of the Children’s Fear Survey Schedule- Dental Subscale (B-CFSS-DS) to assess dental anxiety. Descriptive analyses, chi-squared (X 2 ) tests, and Mann-Whitney U tests were performed, with a significance level of 5%. A total of 51 boys (42.5%) and 69 girls (57.5%) were included. There was no significant difference in dental anxiety between them. However, younger children had higher mean B-CFSS-DS scores (p = 0.036, Mann-Whitney). A higher prevalence of dental anxiety was found in participants from low-income families (p = 0.012, X 2 ) and in patients who did not receive endodontic treatment (p=0.034, X 2 ). Higher mean B-CFSS-DS scores were also observed in participants who did not receive endodontic treatment (p=0.001, Mann-Whitney) compared with those that did receive endodontic treatment. No relationship was found between education level, patient school type, first dental appointment, caries experience, and dental anxiety data. Younger children presented a profile of greater dental anxiety. Socioeconomic factors and dental data exerted some effect on dental anxiety, where children from low-income families and those not subjected to endodontic treatment displayed higher rates of dental anxiety.