ABSTRACT Despite their large size and intimidating appearance, most mygalomorph spiders do not cause severe envenomation in Brazil. “Programa Estadual de Vigilância de Acidentes por Animais Peçonhentos da Secretaria de Estado da Saúde do Paraná”, through the “Sistema de Notificação de Animais Peçonhentos” (SINAP), documents the occurrence of synanthropic species captured by the public and reported to municipal health services. In this study, we present a list of the mygalomorph species received by Secretaria de Saúde do Estado do Paraná (SESA) and their distribution across the state of Paraná, based on the SINAP reports. A total of 575 mygalomorph specimens were identified, originating from all 22 Regional Health Centers (RS) of SESA-PR. We identified species of four families: Actinopodidae (on species: Actinopus itapitocai); Dipluridae (1 species: Diplura catharinensis; Pycnothelidae (two species: Psalistopoides emanueli, and P. fulvimanus); and Theraphosidae (10 species in four genera: Acanthoscurria paulensis, Eupalaestrus campestratus, Grammostola actaeon, Pterinopelma longisternale, Tekoapora wacketi, Vitalius dubius, V. lucasae, V. paranaensis, V. sorocabae and V. vellutinus). Of the aforementioned species, Vitalius paranaensis has the widest distribution, occurring in all Regional Centers of Health except Paranaguá. The other most frequently collected species were Pterinopelma longisternale, Vitalius sorocabae, and Psalistopoides emanueli. In total, the 575 records resulted in only 12 accidents. The results highlight the broad distribution of some species; contributes to the identification of the main species encountered by the population and reported to SINAP in Paraná; and helps to bring attention to the low number of accidents caused by these large-sized spiders. appearance Brazil Programa , Paraná” Sistema SINAP, (SINAP) services study SESA (SESA reports 57 2 RS (RS SESAPR. SESAPR PR. PR SESA-PR families itapitocai itapitocai) 1 ( catharinensis two emanueli P fulvimanus fulvimanus) 10 genera paulensis campestratus actaeon longisternale wacketi dubius V lucasae vellutinus. vellutinus . vellutinus) Paranaguá largesized sized (SINAP 5

ABSTRACT The taxonomic history of Bumba Pérez-Miles, Bonaldo & Miglio, 2014 is mainly based on the inclusion of the new species. Bumba have been characterized by the type IV urticating setae present, retrolateral process on male palpal tibia, palpal bulb resting in a ventral distal excavation of palpal tibia, metatarsus I passes between the two branches of tibial apophysis when flexed, presence of spiniform setae on prolateral and retrolateral sides of maxillae and coxae I-IV. In this paper we include the row of teeth (denticulate row) in the median region of the inferior prolateral keel in all male palps. This structure range from a residual tooth to a ridge of up to five teeth. Both, the denticulate row and the retrolateral process on male palpal tibia in males could be considered as putative synapomorphies for Bumba. Here, Homoeomma humile Vellard, 1924 is transferred to Bumba and redescribed, while the female is described for the first time. Bumba cabocla (Pérez-Miles, 2000) is synonymyzed with B. horrida (Schmidt, 1994). Bumba pulcherrimaklaasi (Schmidt, 1991) is transferred to Cyclosternum Ausserer, 1871. Four new species are described and illustrated: Bumba tapajos sp. nov. from state of Pará, Bumba cuiaba sp. nov. and Bumba rondonia sp. nov., both from states of Rondônia and Mato Grosso, respectively, and Bumba mineiros sp. nov. from Paraguay and the Brazilian states of Goiás, Mato Grosso and Mato Grosso do Sul. Diagnosis of B. horrida and B. lennoni are extended and figures of this species are presented.

The Neotropical Sasoninae Neodiplothele Mello-Leitão, 1917 is revised and now includes eight species. Neodiplothele can be distinguished from other Sasoninae by the absence of the posterior median spinnerets and differs Neotropical relatives as Cosmopelma by the absence of cuspules on coxae of leg I and Paracenobiopelma by the absence of cuspules on the labium. The male of N. irregularis Mello-Leitão, 1917 and N. picta Vellard, 1924 are described and illustrated for the first time. Neodiplothele leonardosi Mello-Leitão, 1939 is considered a junior synonym of N. irregularis. Five new species are described from Brazil: N. aureus sp. nov. from the states of Ceará, Rio Grande do Norte, Paraíba, and Minas Gerais, N. itabaiana sp. nov. from Sergipe, N. martinsi sp. nov. from Bahia, Espírito Santo, and Minas Gerais, N. indicattii sp. nov. from Espírito Santo, Minas Gerais, Rio de Janeiro, and São Paulo, N. caucaia sp. nov. from Ceará, Goiás, and Mato Grosso do Sul. Two informal groups are proposed based on genitalia morphology: irregularis group and picta group. An identification key and new distribution records for all known species are given.

Two new Nemesiidae species from Reserva Biológica do Tinguá, Rio de Janeiro, Brazil are described. Acanthogonatus minimussp. nov. differs from the remaining species of the genus by the male palpal bulb, which has very long and twisted embolus, ca. 2/3 the length of the palpal tibia, long and twisted spermathecae, anterior eye row recurved and fovea T-shaped. Chaco tinguasp. nov. differs from the remaining species of the genus by the retrolateral megaspine on tibia I, palpal embolus tip hook-shaped, inferior tarsal claw on all legs, absence of pubescence on the carapace and legs. Both species were collected with Winkler extractors in leaf litter. They are the smallest specimens in their respective genera and also among the world Nemesiidae described to date. The smallest male of A. minimussp. nov. measures 4.22 mm and of C. tinguasp. nov. measures 3.85 mm. Data on the phenology of C. tinguasp. nov. is presented.

<title>Abstract</title><p>The article provides a historical report on venomous spider identification, venom obtainment methods and serum production at the Butantan Institute, São Paulo, Brazil. It is based on literature and personnal experience during the last 50 years. This result is the discovery that the real species causing potential severe human accidents were the spiders of the genus <italic>Loxosceles</italic> and <italic>Phoneutria</italic>.</p>

O gênero Pycnothele Chamberlin, 1917 é revisado. As espécies brasileiras Pycnothele perdita Chamberlin, 1917, P. singularis (Mello-Leitão, 1934) e P. auronitens (Keyserling, 1891) são redescritas, diagnosticadas e ilustradas. As fêmeas de P. auronitens e P. singularis são descritas pela primeira vez e P. auripila (Mello-Leitão, 1946) do Uruguai, atualmente um sinônimo júnior de P. auronitens, é revalidada. Pycnothele piracicabensis (Piza, 1938) é transferida para Rachias Simon, 1892, do qual compartilha a morfologia das estruturas sexuais, restaurando a combinação original. Cinco espécies novas são propostas para o Brasil: Pycnothele rubra sp. nov., P. jatai sp. nov. e P. araraquara sp. nov., do estado de São Paulo, P. arapongas sp. nov., do estado do Paraná, and P. gauderio sp. nov., do estado de Rio Grande do Sul. Após esta revisão, o gênero passa a constar com dez espécies neotropicais. Mapas com a distribuição de todas as espécies é apresentado pela primeira vez e a ocorrência de espécies do gênero é ampliada para o estado do Paraná.

The genus Pycnothele Chamberlin, 1917 is revised. The Brazilian species Pycnothele perdita Chamberlin, 1917, P. singularis (Mello-Leitão, 1934) and P. auronitens (Keyserling, 1891) are redescribed, diagnosed and illustrated. The females of P. auronitens and P. singularis are described for the first time and P. auripila (Mello-Leitão, 1946) from Uruguay, currently a junior synonym of P. auronitens, is revalidated. Pycnothele piracicabensis (Piza, 1938) is transferred to Rachias Simon, 1892, with which it shares the morphology of the sexual structures, restoring the original combination. Five new species are proposed for Brazil: Pycnothele rubra sp. nov., P. jatai sp. nov. and P. araraquara sp. nov., from state of São Paulo, P. arapongas sp. nov., from state of Paraná, and P. gauderio sp. nov., from state of Rio Grande do Sul. After this revision the genus includes ten Neotropical species. Maps with distribution of all species is presented for the first time and the occurrence of species of the genus is enhanced for the Brazilian state of Paraná.

Uma nova espécie de Tmesiphantes Simon, 1892, T. aridai sp. nov., é descrita e ilustrada baseada em três machos e três fêmeas coletadas na Floresta Nacional do Tapajós, Santarém, Estado do Pará, Brasil. Os machos distinguem se de T. perp Guadanucci & Silva, 2012 pelo aspecto do palpo copulador com uma projeção tegular basal inconspícua, apresentando um êmbolo muito delgado, com quilhas mais curtas, não se estendendo até o ápice, e distinguem se de T. nubilus Simon, 1892 pela apófise tibial com dois ramos de tamanho semelhante, o prolateral com um forte espinho na margem retrolateral. As fêmeas se assemelham a T. nubilus pelo aspecto dos receptáculos seminais, porém com uma constrição menos evidente e formato do ápice irregular. A nova espécie representa o primeiro registro de Tmesiphantes para a região amazônica, elevando para oito o número de espécies conhecidas.

A new species of Tmesiphantes Simon, 1892, T. aridai sp. nov., is described and illustrated based on three males and three females collected at the Floresta Nacional do Tapajós, Santarém, state of Pará, Brazil. Males can be distinguished from T. perp Guadanucci & Silva, 2012 by the palpal bulb with an inconspicous tegular basal projection, but presenting a very slender embolus with shorter keels not extending to the tip, and from T. nubilus Simon, 1892 by the tibial apophysis with two similarly sized branches, the prolateral one with a strong spine on the retrolateral margin. Females resemble T. nubilus by the aspect of the seminal receptacle but the constriction near the apex is less evident and apex shape is irregular. The new species represents the first record of Tmesiphantes from the Amazonian region, bringing the total number of species to eight.

In this study the Brazilian Amazonian species of Acanthoscurria Ausserer, 1871 are redescribed: A. geniculata (C.L. Koch, 1841), A. tarda Pocock, 1903, A. juruenicola Mello-Leitão, 1923, A. theraphosoides (Doleschall, 1871). Acanthoscurria simoensi Vol, 2000 and A. insubtilis Simon, 1892, previously known from French Guyana and Bolivia, respectively, are recorded for Brazil by the first time. The females of these two species are described for the first time and a new species, A. belterrensis sp. nov., is described from Belterra, Pará, Brazil. In addition, four synonymies are established: A. transamazonica Piza, 1972 as junior synonym of A. geniculata; A. ferina Simon, 1892 and A. brocklehursti F.O.P.-Cambridge, 1896 of A. theraphosoides; and A. xinguensis Timotheo da Costa, 1960 of A. juruenicola. Acanthoscurria belterrensis sp. nov. resembles A. gomesiana Mello-Leitão, 1923 by the color pattern and structure of sexual organs. The male can be distinguished by the less curved embolus and the very projected prolateral superior and prolateral inferior keels, giving a triangular aspect to the basis of embolus, and the female seminal receptacles presenting a larger and narrower basis.

The type species of the Neotropical Actinopus, A. tarsalis Perty, 1833, is redescribed based on material from the type locality, the state of Piauí, Brazil. The species appears to be restricted to northeastern Brazil and is newly recorded from the state of Sergipe. An old record from the state of Rio Grande do Sul is rejected. Actinopus tarsalis differs from other species of the genus by details of the male copulatory bulb: tegular apophysis absent, robust embolar base, inserted basally at a right angle (90°); embolar apices apex flattened and expanded, arrow-shaped in dorsal view.

Acanthoscurria natalensis Chamberlin, 1917 is redescribed. The following species are considered junior synonyms of A. natalensis: A. cursor Chamberlin, 1917, A. fracta Chamberlin, 1917, A. rondoniae Mello-Leitão, 1923, A. chiracantha Mello-Leitão, 1923, and A. parahybana Mello-Leitão, 1926. All examined specimens, including the types, share the same general aspect, morphology of the sexual organs, color, and measurements. Acanthoscurria natalensis resembles A. paulensis Mello-Leitão, 1923 and A. chacoana Brèthes, 1909 in the general aspect, size, and by the morphology of the sexual organs: male palpal bulb with embolus ending like a shell, due to the prolateral and superior keels. It can be distinguished from these two species by the less developed keels and the longer embolus. The female resembles A. paulensis and A. chacoana by the fused base of the spermathecae and differs by the more evident lobes, projected from base. The distribution of A. natalensis is expanded to Brazilian states covering the Caatinga and the Cerrado biomes

The male of Acanthoscurria paulensis Mello-Leitão, 1923 is redescribed and the female is described for the first time. The male holotype, from Pirassununga, state of São Paulo, Brazil, could not be located and the redescription is based on specimens collected in the type locality. Acanthoscurria atrox Vellard, 1924 is considered a junior synonym of A. paulensis based on their original descriptions and figures of the male palpal bulb, and also supported by the study of several specimens from both type localities, Pirassununga and Campo Grande, state of Mato Grosso do Sul, Brazil. Acanthoscurria guaxupe Piza, 1972 is also considered a junior synonym of A. paulensis based on examination of the holotype. Acanthoscurria paulensis resembles A. chacoana Brèthes, 1909, A. juruenicola Mello-Leitão, 1923, and A. geniculata (C.L. Koch, 1842) by the large size (over 50 mm). The male of A. paulensis resembles that of A. chacoana,A. juruenicola, and A. geniculata by the aspect of the palpal bulb with an embolus ending like a shell, due to the well-developed prolateral inferior and superior keels. It can be distinguished from A. chacoana by the palpal bulb less compact and with a longer embolus and from A. juruenicola and A.geniculata by the absence of a third accessory keel. The female resembles A. chacoana,A. juruenicola, and A. geniculata by the fused base of the spermathecae, and differs by the square or slightly wider than longer base of the spermathecae and by the terminal lobes directed towards each other. New distribution records for A. paulensis are provided from the Brazilian states of Mato Grosso, Goiás, Minas Gerais, Mato Grosso do Sul, Espírito Santo, Paraná, and Rio Grande do Sul.

Two new species of Lycinus Thorell, 1894 are described in this paper, L. portoseguro sp. nov. and L. choros sp. nov. Lycinus portoseguro sp. nov. is based on male and female from Porto Seguro, state of Bahia, Northeastern Brazil. This is the first record of a species of Lycinus from Brazil. Males of L. portoseguro sp. nov. differ from the remaining species of the genus by the presence of a retrolateral megaspine on tibia I and by the short embolus. Females can be distinguished by the scopulae divided on tarsi II. Lycinus choros sp. nov. is based on one male from La Serena, IV Region (Coquimbo), Chile. It is the eighth species described from this country. L. choros sp. nov. resembles L. epipiptus (Zapfe, 1963) by the longer embolus and differs from it by the short palpal tibia and by the scopulae undivided on male tarsi I-II.

A descrição original desta espécie foi baseada numa única fêmea e nenhuma ilustração de seus órgãos reprodutivos foi apresentada. Após coletarmos alguns espécimes identificados como I. seladonium, decidimos redescrever esta espécie e descobrimos que o macho descrito por Mello-Leitão (1923) havia sido erroneamente identificado como pertencente a esta espécie. Assim, neste artigo, é descrito pela primeira vez, o macho de I. seladonium e pela primeira vez as genitálias do macho e da fêmea são detalhadamente ilustradas. Notas sobre o comportamento e a história natural de I. seladonium são apresentadas baseadas em dados de animais vivos observados no campo e em cativeiro. Foi observado um imaturo de I. seladonium construindo um abrigo convexo com pequenos pedaços de casca de árvore, comportamento desconhecido em Aviculariinae. Duas cortes foram observadas e fotografadas. Os comportamentos de tamborilar com as pernas; tamborilar com os pedipalpos do macho sobre o corpo da fêmea; e movimentos dorso-ventrais, do abdômen da fêmea, durante a inserção do êmbolo, são registrados pela primeira vez em Aviculariinae. O tempo médio de duração da corte e da cópula foi de aproximadamente dois minutos.

The original description of this species was based on a single female and no illustrations of reproductive organs were presented. After we collected several specimens identified as I. seladonium, we decided to redescribe this species and discovered that the male, previously described as belonging to this species, was misidentified by MELLO LEITÃO (1923). Thus, in this paper the male of I. seladonium is newly described and the reproductive organs of male and female are described and illustrated in detail. Notes on the behavior and natural history of I. seladonium are presented based on the observation of live specimens in the field and in captivity. An immature of I. seladonium was observed constructing a hinged retreat with silk and little pieces of bark, a behavior not previously seen for other Aviculariinae. Two courtship events were observed and photographed, leg tapping and palpal drumming of the male on female's body and dorsum-ventral movements of the female's abdomen while the male was inserting the embolus were documented to Aviculariinae for the first time. The courtships and mating lasted almost two minutes.

Seis espécies de aranhas migalomorfas, pertencentes a duas famílias foram coletadas no Centro de Pesquisa e Conservação da Natureza Pró-Mata, São Francisco de Paula, Rio Grande do Sul, Brasil. Destas, cinco são novas. Microstigmatidae: Xenonemesia araucaria sp. nov., machos diferem de X. platensis Goloboff, 1988 e X. otti Indicatti, Lucas & Brescovit, 2007 pelo êmbolo do bulbo longo e curvado. Fêmeas diferem das outras espécies do gênero pelo receptáculo seminal longo e sinuoso ou espiralado. Nemesiidae: Acanthogonatus ericae sp. nov. - machos se parecem com A. tacuariensis (Pérez-Miles & Capocasale, 1982) e A. quilocura Goloboff, 1995 pelo êmbolo longo e curvado, com serrilha na região apical, mas diferencia-se pela presença de uma flange no ápice do êmbolo. Fêmeas diferem do restante das espécies de Acanthogonatus pelos ductos copulatórios curtos, originando-se do ápice da base do domo da espermateca. Stenoterommata arnolisei sp. nov. difere do restante das espécies do gênero pela área subapical do bulbo larga, com um grande número de quilhas, estendendo-se da região mediana no palpo e pelo ducto copulatório longo e espermateca originando-se da borda interna do domo da base da genitália da fêmea. S. grimpa sp. nov.: machos aproximam-se dos de S. arnolisei sp. nov. pela forma do bulbo e pelo êmbolo curto, mas diferem pelas quilhas restritas a região apical. Fêmeas diferem do restante das espécies de Stenoterommata pela presença de duas espermatecas no domo que é arredondado e fundido. S. curiy sp. nov.: machos aproximam-se dos de S. grimpa sp. nov. pela forma do bulbo e pelo êmbolo curto, mas diferem pelas quilhas translúcidas e restritas a região distal que é larga. A presença de S. palmar Goloboff, 1995 é confirmada para o Brasil. Machos de S. palmar são distinguidos do restante das espécies do gênero pelo êmbolo longo, delgado e levemente curvado distalmente. Fêmeas são distinguidas pela espermateca de base simples, e originando-se da área do terço basal ou mediano do comprimento e domo basal triangular. Todas as aranhas foram coletadas com armadilhas de solo em seis áreas distintas (duas de floresta com Araucária, duas de floresta secundária e duas de silvicultura de Pinus spp.) em um período de 20 meses. Os dados de abundância entre as áreas foram comparados para S. arnolisei sp. nov. e X. araucaria sp. nov. baseado na média de aranhas capturadas em cada área. Nenhuma preferência de habitat foi notada, sugerindo ampla tolerância das espécies aos habitats. Dados de fenologia foram obtidos diretamente da abundância em cada período de coleta e os resultados sugerem um padrão reprodutivo para o inverno de S. arnolisei sp. nov. e entre a primavera e o verão de X. araucaria sp. nov.

Six mygalomorph spiders species belonging to two families, were collected in the Centro de Pesquisa e Conservação da Natureza Pró-Mata, São Francisco de Paula, state of Rio Grande do Sul, Brazil. Five of those species, five are new. Microstigmatidae: Xenonemesia araucaria sp. nov., males differ from those of X. platensis Goloboff, 1988 and X. otti Indicatti, Lucas & Brescovit, 2007 by the curved and very long embolus in the male palp. Females differ from the remaining species of the genus in the seminal receptacle very long and sinuous or twisted. Nemesiidae: Acanthogonatus ericae sp. nov. - males resemble those of A. tacuariensis (Pérez-Miles & Capocasale, 1982) and A. quilocura Goloboff, 1995 by the serrated, curved and long embolus but are distinguished by the presence of a flange on the apex of the embolus. Females differ from the remaining Acanthogonatus by the short copulatory ducts, arising from the apex of the basal dome. Stenoterommata arnolisei sp. nov. is distinguished from the remaining species of the genus by the large sub-apical area of bulb, with a great number of keels, extending to the median region in the male palp and by the long copulatory ducts and spermathecae arising from internal border of basal dome in the female genitalia. S. grimpa sp. nov.: males resemble those of S. arnolisei sp. nov. by the shape of the palpal bulb and by the short embolus, but differ in the keels, restricted to the apical region. Females differ from remaining Stenoterommata by the presence of two spermathecae in the rounded fused basal dome. S. curiy sp. nov.: males resemble those of S. grimpa sp. nov. by the shape of palpal bulb and the very short embolus, but differ by the translucid keels restricted to the larger distal region. The presence of S. palmar Goloboff, 1995 is confirmed for Brazil. Males of S. palmar are distinguished from the remaining species of the genus by the long, slender and slightly distally curved embolus. Females are distinguished by the single spermathecae arising from the basal third or median area of the long and triangular basal dome. All spiders were collected with pitfall trapping at six distinct areas (two Araucaria forest areas, two secondary forests and two Pinus spp. Silvicultural plantations) over a period of 20 months. Abundance data between areas was compared for S. arnolisei sp. nov. and X. araucaria sp. nov. based on the mean catches of spiders in each area. No clear habitat preference was shown for each of the species, suggesting broad habitat tolerances. Phenological data were obtained through the abundance in each trapping period; results suggest an overwintering reproductive pattern for S. arnolisei sp. nov. and a spring-summer pattern for X. araucaria sp. nov.

É descrito o gênero Guyruita gen. nov. e duas espécies novas do Brasil. Holothele waikoshiemi (Bertani & Araújo, 2005) da Venezuela é transferido para o novo gênero. Guyruita gen. nov. difere dos outros Ischnocolinae pelas seguintes caracterísicas: lábio densamente ocupado por muitas cúspides (mais de 100), tumescência interqueliceral ausente, sigilla esternal posterior distante da margem, unhas tarsais sem dentes, escópula tarsal I e II inteiras (tarso II com uma fileira de cerdas esparsas, as quais não dividem a escópula), III e IV divididas.

The genus Guyruita gen. nov. and two new species from Brazil are described. Holothele waikoshiemi (Bertani & Araújo, 2005) from Venezuela is transferred here to the new genus. Guyruita gen. nov. differs from the remaining Ischnocolinae by the following features: labium densely occupied by a lot of cuspules (more than 100), intercheliceral intumescence absent, posterior sternal sigilla remote from margin, tarsal claws without teeth, tarsal scopula I-II undivided (tarsus II with a line of sparse setae, which does not divide the scopula), III-IV divided.