Abstract The present study aimed to investigate the socioeconomic and obstetric characteristics of adolescent mothers and the complications they cause to maternal and neonatal health. This baseline data analysis of the MINA-Brazil birth cohort was conducted in the municipality of Cruzeiro do Sul, state of Acre, Brazil. The chi-square test was used to compare characteristics of adolescent and adult postpartum women, and multiple Poisson regression models with robust variance were used to assess associated factors. Among the postpartum women, 26.2% (95%CI: 24.0-28.4) were adolescents. Factors associated with childbirth in adolescence included: nine years or less of schooling (adjPR:1.36; 95%CI: 1.14-1.61), belongs to the lowest quartiles of the wealth index (1st quartile: adjPR:1.40; 95%CI: 1.08-1.80) (2nd quartile: adjPR:1.37; 95%CI: 1.08-1.74), primigravidae (adjPR:3.69; 95%CI: 2.98-4.57), low pre-pregnancy BMI (adjPR:1.28; CI95%: 1.04-1.57), urinary tract infection during pregnancy (adjPR:1.25; CI95%: 1.07-1.46) and less than six prenatal consultations (adjPR:1.42; 95%CI: 1.21-1.66). Poverty, little schooling, primigravidae, low pre-pregnancy BMI, urinary tract infection during pregnancy and few prenatal consultations were associated with childbirth during adolescence in a municipality in the Northern region of Brazil.

Resumo O objetivo do estudo foi investigar as características socioeconômicas e obstétricas de parturientes adolescentes e suas complicações sobre a saúde materna e neonatal. Trata-se de uma análise de dados da linha de base da coorte de nascimentos MINA-Brasil conduzida no município de Cruzeiro do Sul, estado do Acre. Utilizou-se teste qui-quadrado para comparar características das puérperas adolescentes com as adultas e modelos múltiplos de regressão de Poisson com variância robusta para avaliar fatores associados. Entre as puérperas estudadas, 26,2% (IC95%: 24,0-28,4) eram adolescentes. Os fatores associados ao parto na adolescência foram ter nove anos ou menos de estudo (RPaj:1,36; IC95%: 1,14-1,61), pertencer aos menores quartis do índice de riqueza (1° quartil: RPaj:1,40; IC95%: 1,08-1,80) (2° quartil: RPaj:1,37; IC95%: 1,08-1,74), ser primigesta (RPaj:3,69; IC95%: 2,98-4,57), baixo IMC pré-gestacional (RPaj:1,28; IC95%: 1,04-1,57), infecção urinária na gravidez (RPaj:1,25; IC95%: 1,07-1,46) e menos de seis consultas de pré-natal (RPaj:1,42; IC95%: 1,21-1,66). Pobreza, baixa escolaridade, primigestação, baixo IMC pré-gestacional, infecção urinária na gestação e menor número de consultas de pré-natal foram associados ao parto na adolescência em município da região Norte do Brasil.

Abstract The present study aimed to investigate the socioeconomic and obstetric characteristics of adolescent mothers and the complications they cause to maternal and neonatal health. This baseline data analysis of the MINA-Brazil birth cohort was conducted in the municipality of Cruzeiro do Sul, state of Acre, Brazil. The chi-square test was used to compare characteristics of adolescent and adult postpartum women, and multiple Poisson regression models with robust variance were used to assess associated factors. Among the postpartum women, 26.2% (95%CI: 24.0-28.4) were adolescents. Factors associated with childbirth in adolescence included: nine years or less of schooling (adjPR:1.36; 95%CI: 1.14-1.61), belongs to the lowest quartiles of the wealth index (1st quartile: adjPR:1.40; 95%CI: 1.08-1.80) (2nd quartile: adjPR:1.37; 95%CI: 1.08-1.74), primigravidae (adjPR:3.69; 95%CI: 2.98-4.57), low pre-pregnancy BMI (adjPR:1.28; CI95%: 1.04-1.57), urinary tract infection during pregnancy (adjPR:1.25; CI95%: 1.07-1.46) and less than six prenatal consultations (adjPR:1.42; 95%CI: 1.21-1.66). Poverty, little schooling, primigravidae, low pre-pregnancy BMI, urinary tract infection during pregnancy and few prenatal consultations were associated with childbirth during adolescence in a municipality in the Northern region of Brazil. health MINABrazil MINA Brazil Sul Acre chisquare chi square women factors 262 26 2 26.2 95%CI 95CI CI 95 (95%CI 24.028.4 240284 24.0 28.4 24 0 28 4 24.0-28.4 adolescents included adjPR1.36 adjPR136 adjPR 1.36 1 36 (adjPR:1.36 1.141.61, 114161 1.14 1.61 , 14 61 1.14-1.61) 1st st quartile adjPR1.40 adjPR140 1.40 40 adjPR:1.40 1.081.80 108180 1.08 1.80 08 80 1.08-1.80 2nd nd adjPR1.37 adjPR137 1.37 37 adjPR:1.37 1.081.74, 108174 1.74 74 1.08-1.74) adjPR3.69 adjPR369 3.69 3 69 (adjPR:3.69 2.984.57, 298457 2.98 4.57 98 57 2.98-4.57) prepregnancy pre adjPR1.28 adjPR128 1.28 (adjPR:1.28 CI95% CI95 1.041.57, 104157 1.04 1.57 04 1.04-1.57) adjPR1.25 adjPR125 1.25 25 (adjPR:1.25 1.071.46 107146 1.07 1.46 07 46 1.07-1.46 adjPR1.42 adjPR142 1.42 42 (adjPR:1.42 1.211.66. 121166 1.21 1.66 . 21 66 1.21-1.66) Poverty 26. 9 028 24.028. 24028 240 24. 284 28. 24.0-28. adjPR1 adjPR1.3 adjPR13 136 1.3 (adjPR:1.3 141 1.141.61 11416 114 1.1 161 1.6 6 1.14-1.61 adjPR1.4 adjPR14 140 1.4 adjPR:1.4 081 1.081.8 10818 108 1.0 180 1.8 8 1.08-1.8 137 adjPR:1.3 1.081.74 10817 174 1.7 7 1.08-1.74 adjPR3 adjPR3.6 adjPR36 369 3.6 (adjPR:3.6 984 2.984.57 29845 298 2.9 457 4.5 5 2.98-4.57 adjPR1.2 adjPR12 128 1.2 (adjPR:1.2 CI9 041 1.041.57 10415 104 157 1.5 1.04-1.57 125 071 1.071.4 10714 107 146 1.07-1.4 142 (adjPR:1.4 211 1.211.66 12116 121 166 1.21-1.66 02 24.028 2402 24.0-28 adjPR1. 13 1. (adjPR:1. 1.141.6 1141 11 16 1.14-1.6 adjPR:1. 1.081. 1081 10 18 1.08-1. 1.081.7 17 1.08-1.7 adjPR3. 3. (adjPR:3. 2.984.5 2984 29 2. 45 4. 2.98-4.5 12 1.041.5 1041 15 1.04-1.5 1.071. 1071 1.07-1. 1.211.6 1211 1.21-1.6 24.02 24.0-2 (adjPR:1 1.141. 1.14-1. adjPR:1 1.081 1.08-1 (adjPR:3 2.984. 2.98-4. 1.041. 1.04-1. 1.071 1.07-1 1.211. 1.21-1. 24.0- (adjPR: 1.141 1.14-1 adjPR: 1.08- 2.984 2.98-4 1.041 1.04-1 1.07- 1.211 1.21-1 (adjPR 1.14- 2.98- 1.04- 1.21-

Resumo O objetivo do estudo foi investigar as características socioeconômicas e obstétricas de parturientes adolescentes e suas complicações sobre a saúde materna e neonatal. Trata-se de uma análise de dados da linha de base da coorte de nascimentos MINA-Brasil conduzida no município de Cruzeiro do Sul, estado do Acre. Utilizou-se teste qui-quadrado para comparar características das puérperas adolescentes com as adultas e modelos múltiplos de regressão de Poisson com variância robusta para avaliar fatores associados. Entre as puérperas estudadas, 26,2% (IC95%: 24,0-28,4) eram adolescentes. Os fatores associados ao parto na adolescência foram ter nove anos ou menos de estudo (RPaj:1,36; IC95%: 1,14-1,61), pertencer aos menores quartis do índice de riqueza (1° quartil: RPaj:1,40; IC95%: 1,08-1,80) (2° quartil: RPaj:1,37; IC95%: 1,08-1,74), ser primigesta (RPaj:3,69; IC95%: 2,98-4,57), baixo IMC pré-gestacional (RPaj:1,28; IC95%: 1,04-1,57), infecção urinária na gravidez (RPaj:1,25; IC95%: 1,07-1,46) e menos de seis consultas de pré-natal (RPaj:1,42; IC95%: 1,21-1,66). Pobreza, baixa escolaridade, primigestação, baixo IMC pré-gestacional, infecção urinária na gestação e menor número de consultas de pré-natal foram associados ao parto na adolescência em município da região Norte do Brasil. neonatal Tratase Trata se MINABrasil MINA Brasil Sul Acre Utilizouse Utilizou quiquadrado qui quadrado estudadas 262 26 2 26,2 IC95% IC95 IC (IC95% 24,028,4 240284 24,0 28,4 24 0 28 4 24,0-28,4 RPaj1,36 RPaj136 RPaj 1,36 1 36 (RPaj:1,36 1,141,61, 114161 1,14 1,61 , 14 61 1,14-1,61) 1° (1 quartil RPaj1,40 RPaj140 1,40 40 RPaj:1,40 1,081,80 108180 1,08 1,80 08 80 1,08-1,80 2° (2 RPaj1,37 RPaj137 1,37 37 RPaj:1,37 1,081,74, 108174 1,74 74 1,08-1,74) RPaj3,69 RPaj369 3,69 3 69 (RPaj:3,69 2,984,57, 298457 2,98 4,57 98 57 2,98-4,57) prégestacional pré gestacional RPaj1,28 RPaj128 1,28 (RPaj:1,28 1,041,57, 104157 1,04 1,57 04 1,04-1,57) RPaj1,25 RPaj125 1,25 25 (RPaj:1,25 1,071,46 107146 1,07 1,46 07 46 1,07-1,46 prénatal natal RPaj1,42 RPaj142 1,42 42 (RPaj:1,42 1,211,66. 121166 1,21 1,66 . 21 66 1,21-1,66) Pobreza escolaridade primigestação prégestacional, gestacional, 26, IC9 (IC95 028 24,028, 24028 240 24, 284 28, 24,0-28, RPaj1 RPaj1,3 RPaj13 136 1,3 (RPaj:1,3 141 1,141,61 11416 114 1,1 161 1,6 6 1,14-1,61 ( RPaj1,4 RPaj14 140 1,4 RPaj:1,4 081 1,081,8 10818 108 1,0 180 1,8 8 1,08-1,8 137 RPaj:1,3 1,081,74 10817 174 1,7 7 1,08-1,74 RPaj3 RPaj3,6 RPaj36 369 3,6 (RPaj:3,6 984 2,984,57 29845 298 2,9 457 4,5 9 5 2,98-4,57 RPaj1,2 RPaj12 128 1,2 (RPaj:1,2 041 1,041,57 10415 104 157 1,5 1,04-1,57 125 071 1,071,4 10714 107 146 1,07-1,4 142 (RPaj:1,4 211 1,211,66 12116 121 166 1,21-1,66 (IC9 02 24,028 2402 24,0-28 RPaj1, 13 1, (RPaj:1, 1,141,6 1141 11 16 1,14-1,6 RPaj:1, 1,081, 1081 10 18 1,08-1, 1,081,7 17 1,08-1,7 RPaj3, 3, (RPaj:3, 2,984,5 2984 29 2, 45 4, 2,98-4,5 12 1,041,5 1041 15 1,04-1,5 1,071, 1071 1,07-1, 1,211,6 1211 1,21-1,6 (IC 24,02 24,0-2 (RPaj:1 1,141, 1,14-1, RPaj:1 1,081 1,08-1 (RPaj:3 2,984, 2,98-4, 1,041, 1,04-1, 1,071 1,07-1 1,211, 1,21-1, 24,0- (RPaj: 1,141 1,14-1 RPaj: 1,08- 2,984 2,98-4 1,041 1,04-1 1,07- 1,211 1,21-1 (RPaj 1,14- 2,98- 1,04- 1,21-

BACKGROUND Malaria is a public health concern in the Amazonian Region, where Anopheles darlingi is the main vector of Plasmodium spp. Several studies hypothesised the existence of cryptic species in An. darlingi, considering variations in behaviour, morphological and genetic aspects. Determining their overall genetic background for vector competence, insecticide resistance, and other elements is essential to better guide strategies for malaria control. OBJECTIVES This study aimed to evaluate the molecular diversity in genes related to behaviour and insecticide resistance, estimating genetic differentiation in An. darlingi populations from Amazonian localities in Brazil and Pacific Colombian region. METHODS We amplified, cloned and sequenced fragments of genes related to behaviour: timeless (tim) and period (per), and to insecticide resistance: voltage-gated sodium channel (Na V ) and acetylcholinesterase (ace-1) from 516 An. darlingi DNA samples from Manaus, Unini River, Jaú River and Porto Velho - Brazil, and Chocó - Colombia. We discriminated single nucleotide polymorphisms (SNPs), determined haplotypes and evaluate the phylogenetic relationship among the populations. FINDINGS The genes per, tim and ace-1 were more polymorphic than Na V . The classical kdr and ace-1 R mutations were not observed. Phylogenetic analyses suggested a significant differentiation between An. darlingi populations from Brazil and Colombia, except for the Na V gene. There was a geographic differentiation within Brazilian populations considering per and ace-1. CONCLUSIONS Our results add genetic data to the discussion about polymorphisms at population levels in An. darlingi. The search for insecticide resistance-related mechanisms should be extended to more populations, especially from localities with a vector control failure scenario. Region spp An aspects competence resistance region amplified (tim , (per) voltagegated voltage gated ace1 ace 1 (ace-1 51 Manaus Colombia SNPs, SNPs (SNPs) ace- observed gene ace1. 1. resistancerelated scenario (per (ace- 5 (SNPs (ace

BACKGROUND Chagas disease (CD), caused by the protozoan Trypanosoma cruzi, is a neglected human disease. It is endemic to the Americas and is estimated to have an economic impact, including lost productivity and disability, of 7 billion dollars per year on average. OBJECTIVES To assess vulnerability to vector-borne transmission of T. cruzi in domiciliary environments within an area undergoing domiciliary vector interruption of T. cruzi in Colombia. METHODS Multi-criteria decision analysis [preference ranking method for enrichment evaluation (PROMETHEE) and geometrical analysis for interactive assistance (GAIA) methods] and spatial statistics were performed on data from a socio-environmental questionnaire and an entomological survey. In the construction of multi-criteria descriptors, decision-making processes and indicators of five determinants of the CD vector pathway were summarily defined, including: (1) house indicator (HI); (2) triatominae indicator (TI); (3) host/reservoir indicator (Ho/RoI); (4) ecotope indicator (EI); and (5) socio-cultural indicator (S-CI). FINDINGS Determination of vulnerability to CD is mostly influenced by TI, with 44.96% of the total weight in the model, while the lowest contribution was from S-CI, with 7.15%. The five indicators comprise 17 indices, and include 78 of the original 104 priority criteria and variables. The PROMETHEE and GAIA methods proved very efficient for prioritisation and quantitative categorisation of socio-environmental determinants and for better determining which criteria should be considered for interrupting the man-T. cruzi-vector relationship in endemic areas of the Americas. Through the analysis of spatial autocorrelation it is clear that there is a spatial dependence in establishing categories of vulnerability, therefore, the effect of neighbors’ setting (border areas) on local values should be incorporated into disease management for establishing programs of surveillance and control of CD via vector. CONCLUSIONS The study model proposed here is flexible and can be adapted to various eco-epidemiological profiles and is suitable for focusing anti-T. cruzi serological surveillance programs in vulnerable human populations.

To infer recent patterns of malaria transmission, we measured naturally acquired IgG antibodies to the conserved 19-kDa C-terminal region of the merozoite surface protein (MSP)-1 of both Plasmodium vivax (PvMSP-1(19)) and Plasmodium falciparum (PfMSP-1(19)) in remote malaria-exposed populations of the Amazon Basin. Community-based cross-sectional surveys were carried out between 2002 and 2003 in subjects of all age groups living along the margins of the Unini and Jaú rivers, Northwestern Brazil. We found high prevalence rates of IgG antibodies to PvMSP-1(19) (64.0 - 69.6%) and PfMSP-1(19) (51.6 - 52.0%), with significant differences in the proportion of subjects with antibodies to PvMSP-1(19) according to age, place of residence and habitual involvement in high-risk activities, defining some groups of highly exposed people who might be preferential targets of malaria control measures. In contrast, no risk factor other than age was significantly associated with seropositivity to PfMSP-1(19). Only 14.1% and 19.3% of the subjects tested for antibodies to PvMSP-1(19) and PfMSP-1(19) in consecutive surveys (142 - 203 days apart) seroconverted or had a three fold or higher increase in the levels of antibodies to these antigens. We discuss the extent to which serological data correlated with the classical malariometric indices and morbidity indicators measured in the studied population at the time of the seroprevalence surveys and highlight some limitations of serological data for epidemiological inference.

The evolution of malaria in Brazil, its morbidity, the malaria control programs, and the new challenges for these programs in the light of the emergence of asymptomatic infection in the Amazon region of Brazil were reviewed. At least six Brazilian research groups have demonstrated that asymptomatic infection by Plasmodium is an important impediment to malaria control, among mineral prospectors in Mato Grosso and riverside communities in Rondônia and, in our group, in the middle and upper reaches of the Negro river, in the state of Amazonas. Likewise, other researchers have studied the problem among indigenous communities in the Colombian, Peruvian, and Venezuelan parts of the Amazon basin, adjacent to Brazil. The frequency of positive results from the polymerase chain reaction (PCR) among asymptomatic individuals has ranged from 20.4 to 49.5%, and the presence of Plasmodium in the thick blood smears, from 4.2 to 38.5%. Infection with Anopheles darlingi has also been demonstrated by xenodiagnosis among asymptomatic patients with positive PCR results. If a mean of 25% is taken for the asymptomatic infection caused by Plasmodium sp. in the Amazon region of Brazil, malaria control will be difficult to achieve in that region with the measures currently utilized for such control.