Abstract The southern region of the Amazon stands out for the growing agricultural development and installation of large hydroelectric projects. Given this scenario, the objective of this study was to quantify the Áreas de Preservação Permanente (APPs), Permanent Preservation Areas, of the water bodies of the Teles Pires river basin according to the current legislation, checking whether there is conflict regarding the use and occupation of these areas, and then check for the occurrence of degradation and identify the environmental fragility of the area. Using the Modified Normalized Difference Water Index (MNDWI), the water bodies located in the study area were delineated and the APPs were delimited according to Law 12,651/2012. To identify the existence of conflicts within these areas, a map of land use and occupation was generated through Maximum Likelihood supervised classification, which was compared with the limits of the APPs, considering conflict areas whose land use is related to anthropic activities. Lastly, the potential and emerging fragility of the area were calculated, considering data of the slope, soil type and land cover/land use. The delimited APP comprised 3.96% of the total area of the basin and it showed a state of low degradation, with 83.83% of the area conserved under native vegetation cover and 15.93% showing conflicting type of use, with the occupation by pastures standing out, and among the APP categories mapped the headwaters were the most impacted. The spatialization of conflicts within the basin indicated that it has a very different conservation pattern, with the most critical areas concentrated in the center-east, where municipalities that have more than 40% of the APP occupied by anthropic activities are located. The north of the basin has areas with higher potential fragility, which is attenuated by the great soil protection. projects scenario , (APPs) Areas legislation MNDWI, MNDWI (MNDWI) 126512012 12 651 2012 12,651/2012 classification Lastly calculated slope coverland 396 3 96 3.96 8383 83 83.83 1593 15 93 15.93 impacted pattern centereast, centereast center east, east center-east 40 protection (APPs (MNDWI 12651201 1 65 201 12,651/201 39 9 3.9 838 8 83.8 159 15.9 4 1265120 6 20 12,651/20 3. 83. 15. 126512 2 12,651/2 12651 12,651/ 1265 12,651 126 12,65 12,6 12,