The immature stages of Labrundinia tenata Roback, 1987 (Diptera: Chironomidae: Tanypodinae) are described and illustrated and the male is redescribed. Larvae were collected in aquatic systems in southeastern Brazil, where they are associated with aquatic macrophytes. The three semaphoronts of L. tenata are recognized: (a) Male by the abdominal segments I pale brown, II-VI with brown transverse band along proximal margin, VII, VIII almost wholly brown; hypopygium brown; anterior margin of sternapodeme rounded, no distinct spur. (b) Pupa by the thoracic horn S-shaped and a shallow preapical groove; abdominal segment VII with two lateral setae. (c) Larva with posteroventral spine group; posterior parapod with single elongate claw serrated on inner margin and numerous simple claws.