ABSTRACT Integrated crop-livestock system (ICLS) can help improve the soil physical attributes, increasing the nutrients cycling and yield potential. We evaluated the effect of forage height management under ICLS on physical soil attributes and water infiltration. Field experiment was carried out for two consecutive years in a randomized complete block design, with three repetitions. Forage was managed under continuous grazing, grazing up to 30 cm (G30), 20 cm (G20) and 10 cm (G10) of height, and control without grazing. Soil bulk density (SBD), microporosity (MIP), macroporosity (MAP), and total porosity (TP) were measured before and after the grazing at 0-5; 5-10 and 10-20 cm layer depth. Water infiltration rate was evaluated using the concentric rings in eight periods (5, 10, 15, 20, 30, 60, 90 e 120 min after beginning of the process). SBD at 0-5 cm layer was not affected by the grazing management, whereas the MIP, MAP, and TP reduced after sheep grazing compared to ungrazed. Water infiltration rate was higher for the G10 management, with greater amount of accumulated infiltrated water. Path analysis showed positive effects of the soil physical attributes under ICLS to the grain yield soybean, allowing the farmers to enhance their profitability.

ABSTRACT EnlistTM technology is a new trait on soybean and corn conferring tolerance to auxinic-type (2,4-D choline) and graminicides (aryloxyphenoxypropionates – FOPs) herbicides. However, the occurrence of volunteer corn tolerant to 2,4-D and FOPs can become a significant weed on soybean cropping systems, affecting the crop yield and reducing seed quality. This research aimed to evaluate herbicides to EnlistTM volunteer corn control and their effects on plant development, crop yield, and physiological seed quality of EnlistTM soybean. Field experiment was performed in a complete randomized block experimental design with four replicates, using a density of 10 plants·m-2 of EnlistTM volunteer corn. Treatments consisted of herbicides applied at 3-4-leaves stage of volunteer corn with haloxyfop-P-methyl, clethodim, pinoxaden, cloransulam-methyl and imazethapyr regarding to a control with and without volunteer corn. The development and yield components of EnlistTM soybean were evaluated and physiological seed testing performed on soybean’s seeds. Clethodim controlled EnlistTM volunteer corn, whereas haloxyfop-P-methyl, pinoxaden, and cloransulam-methyl showed insufficient control levels (30-35%) at 30 days after spray. Imazethapyr exhibited 84% of control of EnlistTM volunteer corn and injured the EnlistTM soybean, affecting its seed viability. EnlistTM volunteer corn interferences negatively the soybean crop yield, yield components, and affects the plant development pattern.

RESUMO: A massa de hectolitro (MH) é o principal parâmetro quantitativo usado nas unidades de recebimento como indicador de qualidade dos grãos de trigo (Triticum aestivum L.). O teor de umidade e o equipamento utilizado podem ser considerados fontes de variação importantes na classificação de um lote, necessitando correto dimensionamento amostral para mensuração da MH. O objetivo dessa pesquisa foi identificar a variabilidade entre equipamentos para avaliação da MH com correção ou não do teor de umidade da massa de grãos e estimar o número ideal de amostras para classificação comercial de lotes de trigo. O experimento foi realizado em delineamento experimental de blocos ao acaso com quatro repetições. Sete lotes de grãos de trigo, oriundos de diferentes cultivares e/ou locais de produção, foram usados para mensuração da MH em três equipamentos (Equipamento “A”, Equipamento “B” e Equipamento “C”), com e sem correção da umidade dos grãos para 13%. Maiores valores da MH foram determinados no Equipamento “A” (77,15) comparado ao Equipamento “B” (75,08) e Equipamento “C” (74,69), com classificação distinta do trigo quanto ao tipo. O teor de umidade afetou a MH, mas não alterou a classificação final do trigo quanto ao tipo. O número ideal de amostras para MH variou de uma a 18 amostras para o menor nível de precisão (MH = média ± 1 kg hl-1) e, de 67 a 1820 amostras para a maior precisão (MH = média ± 0,1 kg hl-1). O Equipamento “B” requer menor número de amostras (podendo chegar a uma) para um mesmo nível de precisão (MH = média ± 1 kg hl-1), enquanto o Equipamento “A” necessita maior número de amostras para a maioria dos lotes. Assim, a correta classificação do trigo pela MH depende de maior número de amostras.

ABSTRACT: Hectolitre weight (HW) is the principal quantitative parameter used by receiving units as an indicator of the quality of wheat grain (Triticum aestivum L.). The moisture content and the equipment used can be considered important sources of variation to assess a batch, requiring correct sizing of the sample used to measure the HW. This research identified the variability between the equipment used to evaluate HW with or without correcting the grain moisture content, and to estimate the ideal number of samples for the commercial classification of wheat batches. The experiment was carried out in a randomised block design with four replications. Seven batches of wheat grain from different cultivars and/or production sites were used to measure the HW using three types of equipment (Equipment ‘A’, Equipment ‘B’ and Equipment ‘C’), with and without correcting the grain moisture content to 13%. Higher values for HW were determined with Equipment ‘A’ (77.15) compared to Equipment ‘B’ (75.08) and Equipment ‘C’ (74.69), classifying the wheat according to type. The moisture content affected the HW, but did not change the final classification of the wheat in terms of type. The ideal number of samples for HW ranged from 1 to 18 at the lowest level of precision (HW=mean±1 kg hl-1) and from 67 to 1820 samples for the highest precision (HW=mean±0.1 kg hl-1). Equipment ‘B’ requires a smaller number of samples (possibly as few as one) for the same level of precision (HW=mean±1 kg hl-1), whereas Equipment ‘A’ requires a greater number of samples for the majority of batches. Correctly classifying wheat by HW therefore depends on a larger number of samples.

Resumo - O objetivo deste trabalho foi avaliar aspectos ecofisiológicos da germinação de sementes em biótipos de sagitária (Sagittaria montevidensis) resistentes e suscetível a herbicidas. O delineamento experimental foi o inteiramente casualizado. Em campos de arroz, foram colhidas sementes de dois biótipos resistentes (SAGMO 10 e SAGMO 32) e de um suscetível (SAGMO 35) aos herbicidas inibidores da acetolactato sintase e do fotossistema II. A liberação da dormência foi realizada com 2,0% de nitrato de potássio, escarificação química (1,0% de H2SO4 por 30 s) e mecânica (lixa), 10 ppm de ácido giberélico, embebição em água por 24 horas, banho-maria a 60ºC e um controle. Avaliaram-se a germinação às temperaturas de 10 a 40°C e a emergência de plântulas aos 0,0, 0,5, 1,0, 2,0, 4,0 e 5,0 cm de profundidade de enterrio. Todos os biótipos apresentaram germinação superior a 70% após escarificação mecânica. A maior taxa de germinação ocorre a 25,9°C para SAGMO 35, o biótipo suscetível, e a 26,2 e 26,5°C, respectivamente, para SAGMO 10 e SAGMO 32, biótipos resistentes. Para todos os biótipos, a maior emergência de plântulas ocorre em sementes posicionadas a 0,5 e 1,0 cm de profundidade de enterrio.

Abstract - The objective of this work was to evaluate the ecophysiological aspects of seed germination in California arrowhead (Sagittaria montevidensis) biotypes resistant and susceptible to herbicides. The experimental design was completely randomized. In paddy rice fields, seeds were collected from two biotypes that are resistant (SAGMO 10 and SAGMO 32) and from one that is susceptible (SAGMO 35) to acetolactate synthase and photosystem II inhibiting herbicides. Seed dormancy release was performed with 2.0% potassium nitrate, chemical (1.0% H2SO4 for 30 s) and mechanical (sandpaper) scarification, 10 ppm gibberellic acid, water imbibition for 24 hours, water bath at 60°C, and a control. Germination was evaluated at temperatures from 10 to 40°C and seedling emergence at 0.0, 0.5, 1.0, 2.0, 4.0, and 5.0 cm burial depths. All biotypes showed a germination higher than 70% after mechanical scarification. The highest germination rate occurs at 25.9°C for SAGMO 35, the susceptible biotype, and at 26.2 and 26.5°C, respectively, for SAGMO 10 and SAGMO 32, the resistant biotypes. For all biotypes, the highest seedling emergence occurs in seeds positioned at the 0.5 and 1.0 cm burial depths.

ABSTRACT: Italian ryegrass (Lolium multiflorum Lam.) is a troublesome weed widely found in temperate Brazilian fields with several herbicide-resistant populations. Soil seed bank is the main source of future infestations and can be strongly affected by agricultural practices, such as crop rotation and tillage system. Therefore, this study evaluated the long-term effects of different crop rotation and tillage systems on the seed bank of Italian ryegrass. Data were collected in 2012 and 2013 in a field managed since 1985 under conventional-tillage and no-tillage and subjected to three cropping systems (monoculture, total, and partial rotation) compared to the control plot. The samples were collected at three soil depths (0-5, 5-10, and 10-20 cm) using a cylindrical soil sampler, and infestations in the field were evaluated with a sample frame of 1 m2. All cropping systems reduced the soil seed bank of Italian ryegrass, regardless of soil management and sampling depths comparing to the control plot. Total and partial rotation were more effective than soil tillage to reduce the seed bank. A larger number of seeds was observed on the surface layer (0-5 cm) for all cropping systems with more uniform distribution in the soil profile in conventional-tillage. Infestation of Italian ryegrass was greater for monoculture under no-tillage in both years, whereas in total and partial rotation, regardless of soil tillage, infestations remained below 40 seedlings m−2. Thus, strategies to reduce the soil seed bank and infestations of Italian ryegrass should focus on cropping systems with crop rotation and no-tillage.

ABSTRACT: Italian ryegrass (Lolium multiflorum Lam.) is a weed broadly found in fields cultivated with wheat and barley crops. Seed inputs into the soil before harvesting winter crops increase seed bank, ensuring survival. This study evaluated the persistence of Italian ryegrass seeds subjected to burial depths over time. Experiments were carried out in the field for two years in randomized block experimental design with four repetitions. Dormant seeds harvested from mother-plants were put in nylon bags containing soil. Previously, seed viability was evaluated in a 1 % tetrazolium solution, and 50 viable seeds by repetition were buried at 0.5, 5.0, 10, and 20 cm depths. Seed persistence was evaluated by the percentage of deteriorated and remaining seeds, non-dormant seeds, abnormal seedlings, and viable and non-viable dormant seeds at 0, 30, 60, 90, 180, 360, 540, and 720 days after burial (DAB). Seed persistence increased at 10 and 20 cm of burial depth compared to seeds in the soil layers up to 5 cm. Moreover, burial depth at 10 and 20 cm showed lower percentage of deteriorated seeds (10 % lower) compared to 0.5 cm at 360 DAB. For non-dormant seeds, a higher percentage was found at 90 DAB, regardless of seed burial depth. Dormancy breakage occurred until 180 DAB, and more rapidly at 10 and 20 cm depths. At 540 DAB, more than 95 % of seeds were unviable, demonstrating short persistence of Italian ryegrass in the soil seed bank.

ABSTRACT Italian ryegrass (Lolium multiflorum Lam.) is a grass weed found in winter cereals, with several glyphosate-resistant diploid populations. Furthermore, the use of Italian ryegrass tetraploid genotypes creates an opportunity to increase the forage demand in replacement of diploid populations. The aim of this research was to evaluate the crossing capacity between glyphosate-resistant diploid and tetraploid Italian ryegrass to transfer glyphosate resistance to the next generation. Reciprocal crosses were performed using the susceptible tetraploid cultivar INIA Titan and resistant diploid (SVA04 biotype) plants to obtain the F1 progeny. Germination testing, survival percentage, inheritance of glyphosate-resistance, and ploidy level were evaluated in F1 progeny. Crossing of susceptible tetraploid plants ♂ and glyphosate-resistant diploid ♀ affected the germination and exhibited 4.5% of triploid plants. F1 progeny from the glyphosate-resistant diploid ♂ and susceptible tetraploid ♀ did not show germination problems, but 46% of the plants died during the initial seedling stage. For this progeny, 10 and 25% of the seedlings were composed of triploid and pentaploid individuals, respectively. Crosses between susceptible tetraploid ♀ and resistant diploid ♂ did not transfer the glyphosate-resistant for the plants. Susceptible tetraploid ♂ crossing with diploid resistant ♀ had a reduction of 47% the glyphosate-resistant plants compared to the homozygous glyphosate-resistant progeny. Overall, tetraploid Italian ryegrass can be a safe tool to reduce and manage the increase of glyphosate-resistant diploid ryegrass in field conditions.

ABSTRACT Biochemical assays in a time-course were employed to evaluate stresses induced by glyphosate treatment in resistant and sensitive biotypes of Conyza bonariensis. Two experiments were conducted assessing glyphosate doses and time-course after treatment. The doses of glyphosate ranged from 0 to 11840 g ae·ha–1 and assessments performed until 552 h after glyphosate treatment (HAT). The objectives of this study were to evaluate the oxidative stress and differential antioxidant enzyme activity in glyphosate-resistant and -sensitive biotypes of hairy fleabane after glyphosate treatment. After treatment, both studied biotypes accumulated similar levels of shikimic-acid until 96 h. The sensitive biotype died at192 HAT. Shikimic-acid and hydrogen peroxide (H2O2) accumulation in glyphosate-resistant biotype were transient and did not differ from untreated plants at 288 and 500 HAT, respectively. In both glyphosate-resistant and -sensitive biotypes, a correlation analysis established a cause-and-effect relationship after glyphosate treatment, which leads to shikimic-acid and hydrogen peroxide accumulation, lipid peroxidation (indicates tissue damage) and an enhancement in the activity of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) enzyme. However, in the glyphosate-resistant biotype, the oxidative stress and tissue damage were lower, and antioxidant enzyme activities SOD, CAT, and APX were higher than in the -sensitive biotype. It indicates that antioxidant enzyme in glyphosate-resistant biotype might be related to the glyphosate-resistance process in Conyza bonariensis. This study is the first report of differential antioxidant enzyme activity in hairy fleabane.

Resumo: Os objetivos foram avaliar doses de herbicidas em diferentes épocas de dessecação pré-colheita do trigo (Experimento 1); e, o efeito de herbicidas em diferentes épocas de dessecação pré-colheita e épocas de colheita (Experimento 2) sobre a produtividade, germinação e resíduos de herbicidas em sementes de trigo. No experimento 1, os tratamentos foram: duas doses e testemunha sem aplicação dos herbicidas glufosinate, glyphosate, paraquat, paraquat+diuron; e, as épocas de aplicação foram: grão leitoso a pastoso, grão pastoso a farináceo e grão farináceo duro. No experimento 2, os tratamentos foram as épocas de aplicação: grão leitoso a pastoso e grão pastoso a farináceo; os herbicidas: glufosinate, 2,4-D+glyphosate e testemunha sem aplicação; e, as épocas de colheitas: 5, 10 e 15 dias após aplicação. Avaliaram-se massa de mil sementes, produtividade, primeira e contagem final da germinação e resíduo dos herbicidas nas sementes. A dessecação pré-colheita com paraquat, glufosinate e 2,4-D+glyphosate em estádio de grão leitoso a pastoso reduz a produtividade do trigo. Independente do herbicida e dose, aplicação em estádio de grão leitoso a pastoso e grão pastoso a farináceo proporciona maior germinação de sementes de trigo, exceto a menor dose de paraquat. Herbicidas sistêmicos acumulam-se mais nas sementes de trigo.

Abstract: The goals of this study were to evaluate herbicide application rates at different timings for preharvest desiccation of wheat (Trial 1), as well as to evaluate the effect of the timing of herbicide desiccation at preharvest and harvest timing (Trial 2) on yield, germination, and herbicide residue in wheat seed. In Trial 1, treatments consisted of two application rates of glufosinate, glyphosate, paraquat, or paraquat+diuron and a control without application; application time periods were in the milk grain to early dough stage, soft dough to hard dough stage, and hard dough stage. In Trial 2, treatments consisted of different application time periods (milk grain to early dough stage, and soft dough to hard dough stage), different herbicides (glufosinate, 2,4-D+glyphosate, and untreated control), and different harvest times (5, 10 and 15 days after herbicide application). One thousand seeds weight, yield, first and final germination count, and herbicide residue on seeds were evaluated. Preharvest desiccation with paraquat, glufosinate, and 2,4-D+glyphosate at the milk grain to early dough stage reduces wheat yield. Regardless of the herbicide and application rate, application in the milk grain to early dough stage and soft dough to hard dough stage provides greater germination of wheat seeds, except at the lower dose of paraquat. Systemic herbicides accumulate more in wheat seeds.

RESUMO Com o objetivo obter a propagação in vitro de Casearia sylvestris, árvore nativa das florestas brasileiras, estudos de germinação in vitro das sementes foram realizados e as plântulas assépticas obtidas foram usadas como doadoras de explantes na micropropagação. Sementes maduras e imaturas, armazenadas por 10 dias a 10 ou 25°C foram inoculadas em meio contendo 10% de sais de MS e mantidas na luz (fotoperíodo de 16 horas) ou escuro contínuo, a 25°C. Para sementes imaturas, testou-se ou não ácido giberélico (GA3) na concentração de 1,5 mg l-1. Das plântulas obtidas, segmentos cotiledonares e apicais foram retirados e cultivados em meio MS completo com cinco combinações de 6-benzilaminopurina (BAP) e ácido naftalenoacético (ANA): 0,0; 0,25; 0,5; 1,0; 2,0 e 0,0; 0025; 0,05; 0,1; 0,2 mg L-1, respectivamente. As brotações aéreas formadas in vitro foram cultivadas em meio MS contendo 0,0 ou 1,5 mg L-1 de ácido indol-3-butírico (IBA) para o enraizamento. O maior percentual de germinação (60%) ocorreu em sementes maduras expostas à luz, independentemente dos demais tratamentos, podendo ser consideradas fotoblásticas positivas. A maior percentagem de germinação das sementes imaturas (27%) ocorreu quando previamente armazenadas a 10°C, com ou sem GA3. A indução de brotos aéreos foi mais eficiente em segmentos apicais e em meio contendo a menor combinação de BAP e ANA ou isento destes reguladores de crescimento. O maior número de raízes ocorreu em brotos obtidos a partir de segmentos cotiledonares, com ou sem AIB. Após a aclimatização, 83% das plantas sobreviveram.

ABSTRACT In order to do an in vitro propagation of Casearia sylvestris, a tree native to Brazilian forests, studies of in vitro seed germination were performed and aseptic seedlings obtained were used as explant donors for micropropagation. Mature and immature seeds, stored for 10 days at 10 or 25°C were inoculated in medium containing 10% of MS salts and kept in the light (a 16-hour photoperiod) or continuous dark, at 25°C. For immature seeds, gibberellic acid (GA3) was or was not tested at a concentration of 1.5 mg l-1. From the seedlings obtained, the cotyledon and apical segments were removed and cultured in complete MS medium with five combinations of 6-benzylaminopurine (BAP) and naphthalene acetic acid (NAA): 0.0; 0.25; 0.5; 1.0; 2.0 and 0.0; 0025; 0.05; 0.1; 0.2 mg L-1, respectively. The aerial shoots formed in vitro were cultured in MS medium containing 0.0 or 1.5 mg L-1 indole-3-butyric acid (IBA) for rooting. The highest percentage of germination (60%) occurred in mature seeds exposed to light, regardless of other treatments, and may be considered positive photoblastic. The highest percentage of germination of immature seeds (27%) occurred when previously stored at 10 ° C with or without GA3. The induction of aerial shoots was more efficient in apical segments in a medium containing the lowest combination of BAP and NAA or free of these growth regulators. The higher number of roots occurred in aerial shoots obtained from cotyledon segments, with or without IBA. After acclimatization, 83% of the plants survived.

RESUMO. Os inibidores da acetolactato sintase são o principal grupo de herbicidas usados em culturas de inverno do Sul do Brasil onde seu uso intenso selecionou biótipos resistentes de nabo. A resistência afeta a eficácia dos herbicidas, e a identificação do mecanismo de resistência envolvido é importante na definição de estratégias de manejo. O objetivo deste estudo foi elucidar o mecanismo de resistência em biótipos de nabo através da quantificação da atividade da enzima, sequenciamento do gene ALS e avaliar a resposta dos biótipos à aplicação do herbicida iodosulfurom e imazetapir após tratamento com inibidores do metabolismo da citocromo P450 monooxigenase. Os biótipos suscetível (B1) e resistentes (B4 e B13) eram de lavouras de trigo da região Noroeste do Estado do Rio Grande do Sul. Os resultados demonstraram que a afinidade da enzima pelo substrato (KM) não foi afetada nos biótipos B4 e B13, porém o Vmax dos biótipos resistentes foi superior quando comparado ao biótipo B1. Os biótipos resistentes não apresentaram resposta metabólica diferencial ao herbicida iodosulfurom e imazetapir quando inibidos pelo malathion e butóxido de piperolina. Entranto, o sequenciamento do gene ALS evidenciou mutação na posição 574 com uma substituição do aminoácido triptofano por leucina (Trp-574-Leu) nos biótipos resistentes.

ABSTRACT. Acetolactate synthase inhibitors are the main group of herbicides used in winter crops in Southern Brazil where their intensive use has selected for herbicide-resistant biotypes of radish. The resistance affects the efficacy of herbicides, and identifying the resistance mechanism involved is important for defining management strategies. The aim of this study was to elucidate the resistance mechanism of radish biotypes by quantifying the enzyme activity, ALS gene sequencing and evaluating the response of biotypes to iodosulfuron and imazethapyr herbicide application after treatment with a cytochrome P450 monooxygenase inhibitor. The susceptible (B1) and resistant (B4 and B13) biotypes were from wheat fields in the Northwest of Rio Grande do Sul State. The results demonstrated that the enzyme affinity for the substrate (KM) was not affected in biotypes B4 and B13 but that the Vmax of the resistant biotypes was higher than that of biotype B1. The resistant biotypes showed no differential metabolic response to iodosulfuron and imazethapyr herbicides when inhibited by malathion and piperonyl butoxide. However, gene sequencing of ALS showed a mutation at position 574, with an amino acid substitution of tryptophan for leucine (Trp-574-Leu) in resistant biotypes.