Photolytic degradation of fluoxetine (FLX), a medicine commonly known as Prozac®, was evaluated by using different photochemical processes. The ultraviolet/microwave (UV/MW) process showed higher efficiency in all the aspects evaluated in this study. The energy consumption was equivalent to 1.94 × 10-4 kW h mg-1 L (UV/MW), while in the UV process the value was 1.20 × 10-2 kW h mg-1 L. The degradation kinetics were applied to the FLX, with rate constant (k) = 0.15 ± 0.01 min-1 and linear correlation coefficient (R2) = 0.980 for UV, and k = 6.15 ± 0.08 min-1 and R2 = 0.998 for UV/MW. The FLX degradation of 99.16% (UV/MW 5 min) and 98.90% (UV 120 min) were observed, evidencing higher efficiency for the first process. The monitoring of transformation products (TPs) through chromatographic analysis enabled the identification of 9 TPs, proving that for the UV/MW process, the hydroxylated structures are verified in high quantity.

Eight petroleum samples from Potiguar Basin were submitted for determination of saturated biomarkers by gas chromatography (fingerprint), liquid chromatography (saturates, aromatics, resins and asphaltenes), gas chromatography-mass spectrometry (GC-MS), nickel and vanadium by high resolution continuum source graphite furnace atomic absorption spectrometry (HR-CS GF AAS). The geochemical parameters provided information on type of depositional paleoenvironment in which organic matter that gave rise to each oil sample and level of thermal maturation reached by generating rocks that make up the Potiguar Basin were deposited. The relationships between the stable isotopes of carbon (δ13C) and between Ni and V concentrations of each petroleum sample were also evaluated. The evaluations separated for oil samples into two distinct groups. Group 1 formed by samples P1, P2, P5 and P8, with characteristics of generating rock that was deposited in a marine hypersaline environment. Group 2 composed of samples P3, P4, P6 and P7 with freshwater lacustrine depositional paleoenvironment, which were elucidated through the geochemical parameters studied. The hierarchical cluster analysis was presented as promising chemometric tool for characterization of petroleum samples in the according to their origin of the generating rock by ratio parameters of hopane/sterane , tetracyclic polyiprenoids/diastereomers and diastereos R+S/cholestane R+S.

Abstract The objective of this study was to evaluate the effects of Cymbopogon citratus essential oil and its main compound (citral) against primary dental colonizers and caries-related species. Chemical characterization of the essential oil was performed by gas chromatography/mass spectroscopy (GC/MS), and the main compound was determined. Antimicrobial activity was tested against Actinomyces naeslundii, Lactobacillus acidophilus, S. gordonii, S. mitis, S. mutans, S. sanguinis and S. sobrinus. Minimum inhibitory and bactericide concentrations were determined by broth microdilution assay for streptococci and lactobacilli reference, and for clinical strains. The effect of the essential oil on bacterial adhesion and biofilm formation/disruption was investigated. Negative (without treatment) and positive controls (chlorhexidine) were used. The effect of citral on preformed biofilm was also tested using the same methodology. Monospecies and microcosm biofilms were tested. ANOVA or Kruskal-Wallis tests were used (α=0.05). Cytotoxicity of the essential oil to human keratinocytes was performed by MTT assay. GC/MS demonstrated one major component (citral). The essential oil showed an inhibitory effect on all tested bacterial species, including S. mutans and L. acidophilus. Essential oil of C. citratus (10X MIC) reduced the number of viable cells of lactobacilli and streptococci biofilms (p < 0.05). The essential oil inhibited adhesion of caries-related polymicrobial biofilm to dental enamel (p < 0.01). Citral significantly reduced the number of viable cells of streptococci biofilm (p < 0.001). The essential oil showed low cytotoxicity to human keratinocytes. Based on these findings, this study can contribute to the development of new formulations for products like mouthwash, against dental biofilms.

Objetivo: Avaliar a percepção de saúde de idosos residentes em um município do interior do Rio Grande do Sul. Método: Estudo quantitativo, descritivo de corte transversal, onde foi aplicado um questionário sociodemográfico e clínico, em uma população de 274 idosos no período de agosto de 2011 a setembro de 2012. Resultados: Houve predominância do sexo feminino, na faixa etária dos 60-69 anos de idade, baixa renda e escolaridade. Em relação a patologias, 77,38% relataram possuir alguma doença associada, sendo a hipertensão a mais prevalente, acometendo 56,20% dos idosos, seguida da osteoartrite com 28,10%. Relataram ser etilistas 13,14% e tabagistas, 6,57%. Quanto à prática de atividade física, 67,52% relataram realizar atividade física de forma regular. Do total de idosos entrevistados, 47,81% consideraram sua saúde boa. Conclusão: Apesar de a maioria dos idosos ter referido boa saúde, grande número deles relatou ser portador de doenças crônicas, o que reforça a ideia de que a percepção de ser doente está mais relacionada às incapacidades do que a ser portador de danos crônicos.

Objectives: To assess the health perception of elderly living in a town in Rio Grande do Sul state, Brazil. Methods: Quantitative, descriptive cross-sectional study in which a sociodemographic and clinical questionnaire was administered in a population of 274 elderly from August 2011 to September 2012. Results: There was a predominance of females, aged 60-69 years, low income and education. In relation to pathologies, 77.38% reported having chronic disease, hypertension being the most prevalent, affecting 56.20% of the elderly, followed by 28.10% with osteoarthritis. Only 13.14% reported being drinkers and 6.57% reported being smokers. As for the practice of physical activity, 67.52% reported physical activity on a regular basis. Of the total elderly respondents, 47.81% considered their health good. Conclusion: Although most elderly have mentioned good health, a large proportion being reported chronic diseases, which reinforces the idea that the perception of being sick is more related disability than being a carrier of chronic damage.

JUSTIFICATIVA E OBJETIVOS: Dentre as condições dolorosas crônicas que acometem o sistema musculoesquelético destacam-se a síndrome da dor miofascial e a fibromialgia; enquanto a fibromialgia corresponde a uma condição dolorosa difusa, a síndrome da dor miofascial é caracterizada pelo envolvimento localizado. O objetivo deste estudo foi revisar os estudos da literatura, a fim de identificar e agrupar informações sobre a síndrome da dor miofascial e a fibromialgia. CONTEÚDO: Foram selecionados 29 artigos de por meio da consulta aos indexadores de pesquisa nas bases de dados eletrônicas Medline, LILACS e Scielo publicados nas línguas inglesa e portuguesa, utilizando os descritores myofascial pain syndrome/ síndrome da dor miofascial, fibromyalgia/ fibromialgia, physical therapy in myofascial pain syndrome/ fisioterapia na síndrome da dor miofascial and/e physical therapy in fibromyalgia/ fisioterapia na fibromialgia, publicados no período de 1991 a 2012. CONCLUSÃO: Os programas de fisioterapia promovem os maiores ganhos na diminuição do impacto dos sintomas da síndrome da dor miofascial e da fibromialgia na vida dos pacientes, daí a importância do trabalho multidisciplinar e educativo no qual o fisioterapeuta, participa informando e instruindo corretamente os pacientes.

BACKGROUND AND OBJECTIVES: Myofascial pain syndrome and fibromyalgia are among chronic pain conditions affecting the musculoskeletal system. While fibromyalgia is a diffuse pain, miofascial pain syndrome is characterized by its localized involvement. This study aimed at reviewing the literature to identify and group information about myofascial pain syndrome and fibromyalgia. CONTENTS: Electronic Mediline, LILACS and Scielo databases were queried in the search for articles published in English and Portuguese, using the keywords myofascial pain syndrome / síndrome de dor miofascial, fibromyalgia / fibromialgia, physical therapy in myofascial pain syndrome / fisioterapia na síndrome de dor miofascial, and physical therapy in fibromyalgia / fisioterapia na fibromialgia, published from 1991 to 2012. Twenty-nine articles were selected. CONCLUSION: Physical therapy programs promote more gains to decrease the impact of myofascial pain syndrome and fibromyalgia symptoms on patients' lives, thus the importance of the multidisciplinary and educative work, where the physical therapist participates, accurately informing and guiding patients.

A evolução das plantas cultivadas, que teve início há cerca de 13.000 anos, está sujeita aos mesmos processos evolutivos naturais, aliada à ação do homem de forma consciente ou inconsciente, levando à domesticação. Nesta revisão, são apresentados os principais fatores evolutivos, tais como mutação, hibridação, migração, seleção e deriva genética, que, de alguma maneira, estão envolvidos com a origem, evolução e domesticação de plantas cultivadas. São apresentados também exemplos de como esses processos influenciaram na diversidade intra e interespecífica de plantas cultivadas, com o aparecimento de novas variedades ou mesmo de novas espécies. De modo geral, tais processos atuaram na ampliação, na manutenção, bem como na redução da variabilidade genética das plantas cultivadas.

The evolution of crop plants, which began at about 13,000 years ago, is subject to the same natural evolutionary processes, coupled with the action of man, consciously or unconsciously, leading to domestication. This review presents the main evolutionary factors such as mutation, hybridization, migration, selection and genetic drift, which somehow are involved in the origin, evolution and domestication of crop plants. Examples of how these processes influenced in the intra and interespecific diversity of crop plants, with the uprise of new varieties or even of new species, are also presented. In general, these processes have worked well in the increase, maintenance, as well as in the reduction of genetic diversity of crop plants.

Este estudo teve como objetivo avaliar a diversidade genética de 20 genótipos de mandioca (Manihot esculenta Crantz) com alto desempenho fenotípico, utilizando marcadores microssatélites. Foram considerados dois grupos: onze variedades para fins industriais e nove variedades de mesa. A partir de nove iniciadores foi identificado alto polimorfismo para todos os locos analisados, atingindo valores de 100%. Em média, foram encontrados 3,4 alelos por loco, sendo 0,371 o valor estimado para a heterozigosidade observada e 0,555 para a diversidade gênica, no conjunto total de variedades comerciais avaliadas. A variabilidade genética observada em ambos os grupos de variedades, cultivadas em larga escala na Região Centro-Sul do Brasil, é ampla o suficiente para permitir a escolha de genótipos parentais divergentes para serem utilizados em cruzamentos para obter novos genótipos recombinantes. Além disso, as análises revelaram alta variabilidade genética dentro dos dois grupos (I: variedades industriais; II variedades de mesa). Contudo, nas variedades de mesa houve maior diversidade genética, provavelmente devido à alta prioridade dada às características sensoriais. Pela análise de agrupamento, verificamos uma tendência para a separação das variedades industriais das de mesa. Os resultados representam importante fonte de informação para programas de melhoramento de mandioca no Brasil.

This study was carried out in order to assess the genetic diversity of 20 cassava (Manihot esculenta Crantz) genotypes with high phenotypic performance using microsatellite markers. Two groups were considered for this study: eleven varieties for industrial uses and nine for household consumption. By using nine microsatellite primers, high polymorphism was identified in all the loci analyzed, with values reaching 100%. On average, 3.4 alleles per locus were found, with 0.371 the value estimated for the observed heterozygosity and 0.555 for gene diversity for the entire set of varieties. The genetic variability found in both varieties, cultivated on a large-scale in the South Center region of Brazil, is wide enough to allow the choice of divergent parental genotypes to be used in crosses to obtain new recombinant genotypes. Furthermore, the analyses indicated a high genetic variability within the two groups (I: varieties for industrial uses; II: varieties for household consumption). However, varieties for household consumption attain higher genetic variability, probably due to high priority placed on selection of different sensorial traits. In the cluster analysis, a tendency for separation of varieties for industrial use and household consumption was verified. Our results represent an important source of information to the cassava breeding program in Brazil.

Os marcadores microssatélites são úteis para a análise da diversidade genética de variedades tradicionais de batata-doce (Ipomoea batatas). Para estes estudos, métodos práticos de extração de DNA precisam ser estabelecidos para assegurar uma boa qualidade e quantidade de DNA extraído. Assim, foi comparada a eficiência de três metodologias para extração de DNA usando o tampão de extração CTAB, todas com modificações. Para verificar a quantidade e pureza na quantificação de DNA, bem como o padrão de bandas de microssatélites para as três metodologias utilizaram-se seis etnovariedades de batata-doce. Os testes mostraram que as três metodologias apresentaram resultados satisfatórios. Uma das metodologias baseada em tecido foliar macerado em nitrogênio líquido mostrou-se a mais adequada devido à simplicidade e menor custo. Entretanto, o método baseado em tecido foliar seco foi o mais vantajoso devido à praticidade na aquisição da planta e no processo de secagem, principalmente quando a coleção encontra-se em condições in situ, e pela possibilidade do armazenamento refrigerado das amostras secas e maceradas para futuras extrações de DNA.

Microsatellite markers have proved to be useful in genetic diversity assessments of sweetpotato (Ipomoea batatas) but practical DNA extraction methods to ensure good quality and quantity DNA for these studies are yet to be established. This study compares the efficiency of three modified methodologies for DNA extraction of six sweetpotato landraces using the CTAB extraction buffer in regard to quantity and purity of DNA quantification and microsatellite band patterns. All methodologies yielded satisfactory results, but the method based in leaf tissue macerated in liquid nitrogen was deemed more adequate because of its simplicity and lower cost. However, the method based in dry leaf tissue was considered more advantageous, first because elicits practicability in the plant acquisition and drying process, especially when the collection is performed in situ, and also because its simplicity makes possible the cold storage of the dry, ground samples for future DNA extractions.

OBJETIVO: Determinar a atividade sérica de AST, CK e CK-MB em cadelas anestesiadas com cetamina S (+), atropina e xilazina em diferentes associações. MÉTODOS: Vinte e três cadelas saudáveis foram distribuídas ao acaso em quarto grupos denominados GI (n=6), GII (n=6), GIII (n=6) e GIV (n=5) tratados respectivamente com atropina e cetamina S (+) (0,04mg/kg; 10 mg/kg); cetamina S (+) (10 mg/kg); atropina, xilazina e cetamina S (+) (0,04mg/kg; 1,1 mg/kg; 10 mg/kg) exilazina e cetamina S (+) (1,1 mg/kg; 10 mg/kg). A atividade sérica de AST, CK e CK-MB foi determinada antes da pré-medicação (M0) e uma, duas, três seis, 12, 24 e 36 horas após M0. RESULTADOS: Não foram encontradas mudanças significativas na atividade sérica de AST, CK e CK-MB entre grupos. Entretanto, entre momentos houve aumento da atividade sérica de CK para todos os grupos, exceto em GI.Com relação a atividade sérica de CK-MB, observou-se ao longo dos momentos aumento significativo com relação aos valores basais em ambos os grupos. CONCLUSÃO: Alterações significativas foram observadas com relação à atividade sérica de CK e CK-MB em todos os tratamentos, mantendo-se elevada por um período maior nos grupos GIII e GIV.

PURPOSE: To assessment of the aspartate aminotransferase (AST), creatine kinase (CK) and creatine kinase isoenzyme fraction MB (CK-MB) serum activity in female dogs anesthetized with ketamine S (+), atropine and xylazine in several associations. METHODS: Twenty three healthy female dogs randomly distributed in four groups named as GI (n=6), GII (n=6), GIII (n=6) and GIV (n=5) were treated respectively with atropine and ketamine S(+) (0.04mg/kg; 10 mg/kg); ketamine S(+) (10 mg/kg); atropine, xylazine and ketamine S(+) (0.04mg/kg; 1.1 mg/kg; 10 mg/kg) and xylazine and ketamine S(+) (1.1 mg/kg; 10 mg/kg). AST, CK and CK-MB serum activity measurement before pre-medication (M0) and one, two, three, six, 12, 24, 36 hours after. RESULTS: There was no significant change in AST, CK e CK-MB serum activity among groups. However, CK serum activity in relation to moments within the groups was increased in all groups over the time in spite of treatment, except GI. In relation to CK-MB activity, in the moments within the group, it was observed an increase compared to baseline in all groups. CONCLUSION: Creatine kinase and creatine kinase fraction MB isoenzyme showed changes in their mean values remained higher than baseline for a longer time in GIII and GIV.

Based on nine microsatellite loci, the aim of this study was to appraise the genetic diversity of 42 cassava ( Manihot esculenta ) landraces from selected regions in Brazil, and examine how this variety is distributed according to origin in several municipalities in the states of Minas Gerais, São Paulo, Mato Grosso do Sul, Amazonas and Mato Grosso. High diversity values were found among the five above-mentioned regions, with 3.3 alleles per locus on an average, a high percentage of polymorphic loci varying from 88.8% to 100%, an average of 0.265 for observed heterozygosity and 0.570 for gene diversity. Most genetic diversity was concentrated within the regions themselves ( H S = 0.52). Cluster analysis and principal component based scatter plotting showed greater similarity among landraces from São Paulo, Mato Grosso do Sul and Amazonas, whereas those from Minas Gerais were clustered into a sub-group within this group. The plants from Mato Grosso, mostly collected in the municipality of General Carneiro, provided the highest differentiation. The migration of human populations is one among the possible reasons for this closer resemblance or greater disparity among plants from the various regions.

We used simple sequence repeat (SSR) markers to investigate the genetic diversity of 78 sweet potato (Ipomoea batatas) accessions (58 landraces and 20 putative clones) from traditional agricultural households from 19 local communities in the Vale do Ribeira, São Paulo, Brazil. Eight SSR loci were assessed using 6% (w/v) polyacrylamide gels stained with silver nitrate and the accessions genotyped considering the presence or absence of bands. The results were subjected to analysis of molecular variance (AMOVA), and cluster and principal coordinate analyses. Spatial structure was assessed using Mantel's test to compare genetic and geographic distances. Each primer pair generated between three and ten clearly scorable polymorphic fragments. Cluster analyses showed a Jaccard's index from 0.3 to 1.0, indicating high genetic and intravarietal diversity. Accessions from all 19 communities were not spatially structured (r = 0.15, p < 0.054), with AMOVA indicating that most of the variability (58.2%) was distributed within households and only 18.1% of the variability was distributed between households within communities. The outcrossing mating system of sweet potato, and anthropic factors such as selection of different varieties and their maintenance within household small plots and home gardens, as well as an extensive exchange system between agriculturists, may all be contributing to these results.

Avaliou-se a diversidade fenotípica de etnovariedades de batata-doce através de descritores morfológicos, visando verificar como esta diversidade está distribuída em nível de roças e comunidades do Vale do Ribeira, SP, Brasil. Foram avaliados, no total, 74 acessos, envolvendo 53 etnovariedades, coletadas em 30 roças, distribuídas em 18 comunidades de agricultores que praticam agricultura tradicional nos municípios de Iguape, Ilha Comprida e Cananéia, somadas a quatro variedades comerciais adquiridas em varejões de Iguape e Piracicaba. A avaliação foi realizada em condições experimentais ex situ em Piracicaba, SP. Foram avaliados nove descritores fenológicos e florais, nove descritores morfológicos da parte aérea e cinco da raiz. Os 14 descritores de parte aérea e raiz foram transformados em dados binários, totalizando 74 atributos. Foi realizada uma análise de agrupamento, empregando-se o coeficiente de similaridade de Jaccard e o método aglomerativo UPGMA (unweighted pair group method with arithmetic mean). Os dados binários foram também submetidos a uma análise de variância (AMOVA). Não se detectou formação de grupos definidos, indicando que não há estruturação espacial da diversidade para as etnovariedades, mas observou-se grande variação morfológica (índice de Jaccard variando de 0,12 a 1,0) na região estudada. A maior parte da variabilidade encontra-se distribuída dentro de roças (64,4%), seguida pela distribuição entre roças dentro de comunidades (27,1%) e entre comunidades (8,4%). Portanto, os agricultores tradicionais no Vale do Ribeira cultivam em suas roças grande diversidade morfológica de batata-doce, cuja origem pode ter sido gerada pelo sistema reprodutivo da espécie por alogamia, por eventuais mutações somáticas e pelo amplo sistema de trocas entre agricultores em âmbito local e regional.

The phenotypic diversity of sweet potato (Ipomoea batatas) landraces was assessed using morphological traits, verifying how this diversity is distributed among the households and settlements of the Vale do Ribeira, Brazil. A total of 74 accessions, involving 53 landraces, collected from 30 households distributed among 18 settlements that practice traditional agriculture in the municipalities of Iguape, Ilha Comprida, and Cananeia, as well as four commercial varieties acquired in markets of Iguape and Piracicaba, were evaluated under an ex situ experimental condition in Piracicaba, SP, Brazil. Nine phenological and floral descriptors, nine morphological vegetative aerial descriptors and five storage root traits were recorded. The 14 aerial vegetative and root descriptors were evaluated as binary data, totaling 74 attributes. Cluster analyses were made using the Jaccard similarity index and the UPGMA (unweighted pair group method with arithmetic mean) agglomerative method. Binary data was also submitted to a variance analysis (AMOVA). No defined groups were observed, indicating that the diversity of the landraces is not structured in space, but considerable morphological variation was found in this area (Jaccard similarity index varying from 0.12 to 1.0). Most of the variability occurred within households (64.4%), followed by the distribution among households within settlements (27.1%) and among settlements (8.4%). Thus, the traditional agriculturists of Vale do Ribeira maintain a high morphological diversity for sweet potato within their households, which can be assumed to be produced by the outcrossing mating system of this species and somatic mutation events, as well as the exchange system at local and regional levels.

Cistos branquiais são descritos em oito exemplares de Tilapia nilotica, no Estado do Rio de Janeiro. Os cistos epiteliais consistiam de hipertrofia das células do epitélio branquial que mostravam um único e grande corpúsculo de inclusão basofílico. A enfermidade relatada apresenta as características peculiares da doença epiteliocística.

Branchial cysts are described in eight specimens of Tilapia nilotica from the state of Rio de Janeiro, Brazil. Cysts consisted of hypertrophy of branchial epithelial cells, which dysplayed a single basophilic corpuscle. The disease reported has the microscopic hallmarks of epitheliocystis.