ABSTRACT This study aimed to analyse the effects of invasion by the exotic Ligustrum lucidum on mycorrhizal associations in forests of Celtis tala, and to determine the role that these fungi play in the invasion process. We analyzed colonization and response to mycorrhization of both plant species under greenhouse conditions after inoculation with soil obtained from patches of non-invaded native forests and invaded forests. The spores present in pots at the end of the experiment were counted and identified. Colonization of L. lucidum was greater in plants inoculated with invaded forest soil, whereas colonization of C. tala was greater in plants inoculated with non-invaded native forest soil. Principal component analysis of fungal species abundance showed differences in AMF composition according to host plant and forest type. The results show that L. lucidum establishes symbiosis with native AMF, which triggers changes in community structure. These changes favored the mycorrhization of L. lucidum and interfered with the mycorrhization of C. tala. AMF may have either a direct or indirect role in the invasion process, not only benefiting the invasive species but also preventing the regeneration of native plants.

To propose new silvicultural practices, it is necessary to understand the involved ecosystem processes and the impact that they produce. The proposed practices generated several questions related to remnant tree stability, the seedling bank, seed production, regeneration dynamic and the influence of biotic and abiotic damages. The objective was to determine the influence of biotic (forest structure, seedling bank, seed production and browsing by Lama guanicoe) and abiotic factors (damages by freezing or drought) in the growth of pre- and post-harvest regeneration in a Nothofagus pumilio stand under shelterwood cutting over 20 years. Monitoring surveys of forest structure and regeneration dynamics were conducted in ten permanent plots. Results showed that the remaining overstory trees reduce their basal area after harvesting (22.3 to 10.7 m² ha-1), varying their crown cover/basal area ratio (1.1 to 6.5). Also, this study suggest that: (i) recruitment, survival and growth of pre- and post-harvest regeneration is a result of the interaction of several factors, e.g. pre-existing seedling bank, post-harvest seeding production, and the biotic and abiotic damages, being more sensitive during the first four years; (ii) pre- and post-harvest plants are the responsible for generating the future forests; and (iii) abiotic factors (freezing or drought) produce higher growth loss than that produced by biotic ones (browsing). In this sense, permanent plots for long-term monitoring allow identifying the risk factors of the proposed silvicultural practices.

Para definir nuevas prácticas silvícolas es necesario conocer los procesos ecosistémicos involucrados y los impactos que generan. Las propuestas implementadas generaron diversos interrogantes relacionados al dosel remanente, el banco de plántulas, la producción de semillas, la dinámica de la regeneración, y la influencia de daños bióticos y abióticos. El objetivo fue determinar la influencia de factores bióticos (estructura forestal, banco de plántulas, producción de semillas y ramoneo por Lama guanicoe) y abióticos (daños por heladas y desecamientos) en el crecimiento de la regeneración pre- y post-cosecha en un rodal de Nothofagus pumilio intervenido mediante una corta de protección luego de 20 años. Se realizaron monitoreos de estructura forestal y la dinámica de la regeneración en 10 parcelas permanentes. Los resultados determinaron que el dosel remanente disminuyó paulatinamente su área basal después de la cosecha (22,3 a 10,7 m² ha-1), variando su relación cobertura/área basal (1,1 a 6,5) indicando una recuperación. También surgió que: (i) el establecimiento, supervivencia y crecimiento de la regeneración pre- y post-cosecha fue el resultado de la interacción de varios factores, e.g. banco de plántulas pre-existentes, producción de semillas post-cosecha, y los daños bióticos y abióticos, siendo más sensibles durante los primeros cuatro años; (ii) las plantas pre- y post-cosecha son las responsables de generar el bosque futuro; y (iii) los daños abióticos (heladas y desecamientos) produjeron mayor pérdida de crecimiento que los bióticos (ramoneo). En este sentido, las parcelas permanentes de monitoreo a largo plazo permitieron identificar factores de riesgo en las propuestas silvícolas implementadas.