RESUMEN Objetivo: Validar el instrumento de medición que evalúa el uso y la adhesión de los equipos de protección individual entre los residentes de salud. Método: Estudio metodológico desarrollado en un entorno virtual entre agosto de 2020 y marzo de 2021 con residentes de atención primaria y hospitalaria de las cinco regiones de Brasil. Los participantes respondieron a la versión adaptada del “E.P.I.-APS” para residentes. Se utilizó un análisis factorial confirmatorio para validar el instrumento. Resultados: Participaron 227 residentes, en su mayoría mujeres cis (82,8%), que trabajaban en el sureste (58,1%) y en cuidados hospitalarios (47,6%). Se confirmó la estructura en ocho dominios (gorro; guantes; comportamiento de seguridad; mascarilla N95; higiene de las manos; delantal/abrigo; mascarilla quirúrgica; gafas/protector facial). Sólo se mantuvieron dos ítems con una carga factorial inferior a 0,5. Conclusión: La herramienta es válida para medir el uso y la adhesión de los equipos de protección individual entre los residentes, lo que ayuda a orientar la formación y la seguridad laboral. Objetivo salud Método 202 Brasil E.P.I.APS EPIAPS E.P.I. APS E P I “E.P.I.-APS Resultados 22 82,8%, 828 82,8% , 82 8 (82,8%) 58,1% 581 58 1 (58,1% 47,6%. 476 47,6% . 47 6 (47,6%) gorro (gorro guantes N95 N manos delantal/abrigo delantalabrigo delantal abrigo quirúrgica gafasprotector gafas protector facial. facial facial) 05 0 5 0,5 Conclusión laboral 20 EPI E.P.I 2 82,8 (82,8% 58,1 (58,1 47,6 4 (47,6% N9 0, 82, (82,8 58, (58, 47, (47,6 (82, (58 (47, (82 (5 (47 (8 ( (4

ABSTRACT Objective: To validate the measuring instrument that assesses healthcare residents’ use and adherence to personal protective equipment. Method: Methodological study developed in a virtual environment between August 2020 and March 2021 with primary care and hospital residents from the five regions of Brazil. The participants answered the adapted version of the “P.P.E.-PHC” for residents. Confirmatory factor analysis was used to validate the instrument. Results: 227 residents took part, the majority of whom were cis women (82.8%), worked in the Southeast region (58.1%), and in-hospital care (47.6%). The structure in eight domains (cap, gloves, safety behavior, N95 mask, hand hygiene, apron/coat, surgical mask, goggles/face shield) was confirmed. Only two items with a factor load of less than 0.5 were kept. Conclusion: The tool is valid for measuring residents’ use of and adherence to personal protective equipment, which helps to guide training and occupational safety. Objective equipment Method 202 Brazil P.P.E.PHC PPEPHC P.P.E. PHC P E “P.P.E.-PHC Results 22 part 82.8%, 828 82.8% , 82 8 (82.8%) 58.1%, 581 58.1% 58 1 (58.1%) inhospital 47.6%. 476 47.6% . 47 6 (47.6%) cap, cap (cap gloves behavior N N9 mask hygiene aproncoat apron coat apron/coat gogglesface goggles face shield confirmed 05 0 5 0. kept Conclusion 20 PPE P.P.E 2 82.8 (82.8% 58.1 (58.1% 47.6 4 (47.6% 82. (82.8 58. (58.1 47. (47.6 (82. (58. (47. (82 (58 (47 (8 (5 (4 (

RESUMO Objetivo: Validar o instrumento de medida que avalia o uso e a adesão de equipamentos de proteção individual entre os residentes de saúde. Método: Estudo metodológico desenvolvido em ambiente virtual entre agosto de 2020 e março de 2021 com residentes da atenção primária e hospitalar das cinco regiões do Brasil. Os participantes responderam a versão adaptada para residentes do “E.P.I.-APS”. Utilizou-se a análise fatorial confirmatória para validação do instrumento. Resultados: Participaram 227 residentes, sendo maioria mulher cis (82,8%), atuavam na região Sudeste (58,1%) e em atenção hospitalar (47,6%). A estrutura em oito domínios (gorro/touca; luvas; comportamento de segurança; máscara N95; higienização das mãos; avental/capote; máscara cirúrgica; óculos de proteção/protetor facial) foi confirmada. Apenas dois itens com carga fatorial inferior a 0,5 foram mantidos. Conclusão: O instrumento é válido para mensurar o uso e adesão aos equipamentos de proteção individual entre residentes, o que contribui para direcionar a formação e a segurança laboral. Objetivo saúde Método 202 Brasil E.P.I.APS. EPIAPS E.P.I. APS . E P I “E.P.I.-APS” Utilizouse Utilizou se Resultados 22 82,8%, 828 82,8% , 82 8 (82,8%) 58,1% 581 58 1 (58,1% 47,6%. 476 47,6% 47 6 (47,6%) gorro/touca gorrotouca gorro touca (gorro/touca luvas N95 N mãos avental/capote aventalcapote avental capote cirúrgica proteçãoprotetor protetor facial confirmada 05 0 5 0, mantidos Conclusão laboral 20 E.P.I.APS EPI E.P.I “E.P.I.-APS 2 82,8 (82,8% 58,1 (58,1 47,6 4 (47,6% N9 82, (82,8 58, (58, 47, (47,6 (82, (58 (47, (82 (5 (47 (8 ( (4

RESUMO Objetivos: refletir sobre o cotidiano dos familiares codependentes de substâncias psicoativas e o papel dos profissionais de saúde na Atenção Básica. Métodos: investigação descritiva delineada na abordagem fenomenológica de Maffesoli, realizada em uma Unidade Básica de Saúde mineira com oito familiares codependentes de substâncias psicoativas. Foram coletados dados de caracterização sociodemográfica e entrevistas em profundidade, que foram analisadas com apoio do software NVivo Pro11®. Atenderam-se os aspectos ético-legais. Resultados: o profissional mais mencionado no discurso das participantes foi o médico, que assumiu uma dubiedade de papéis junto aos demais profissionais de saúde: de acolhimento (percepções positivas sobre a assistência recebida) e de descaso (percepções negativas). Considerações Finais: a dependência de substâncias psicoativas acarreta ônus à pessoa, aos seus familiares e aos profissionais de saúde. É indissociável o papel do estabelecimento de boas relações na tríade profissionais-usuários-familiares da Atenção Básica em prol do tratamento e preservação da saúde mental dos envolvidos.

ABSTRACT Objectives: to reflect on the daily life of family members dependent on psychoactive substances and the role of health professionals in Primary Care. Methods: a descriptive investigation outlined in Maffesoli’s phenomenological approach, carried out at a Basic Health Unit in Minas Gerais with eight family members dependent on psychoactive substances. Sociodemographic characterization data and in-depth interviews were collected and analyzed with support of NVivo Pro11® software. All ethical and legal aspects were met. Results: physicians were the professionals most mentioned in participants’ statements, who took on a dubious role with other health professionals: welcoming (positive perceptions about care received) and neglect (negative perceptions). Final Considerations: dependence on psychoactive substances imposes a burden on persons, their family members and health professionals. The role of establishing good relationships in the triad professionals-users-family members of primary care is inseparable in favor of treatment and preservation of their mental health.

RESUMEN Objetivos: reflexionar sobre el día a día de los familiares dependientes de sustancias psicoactivas y el papel de los profesionales sanitarios en Atención Primaria. Métodos: investigación descriptiva perfilada en el abordaje fenomenológico de Maffesoli, realizada en una Unidad Básica de Salud en Minas Gerais con ocho familiares dependientes de sustancias psicoactivas. Se recolectaron datos de caracterización sociodemográfica y entrevistas en profundidad, que fueron analizadas con el apoyo del software NVivo Pro11®. Se cumplieron los aspectos éticos y legales. Resultados: el profesional más mencionado en el discurso de los participantes fue el médico, quien asumió un rol dudoso con el resto de profesionales de la salud: acogida (percepciones positivas sobre la asistencia recibida) y desatención (percepciones negativas). Consideraciones Finales: la dependencia de sustancias psicoactivas supone una carga para la persona, sus familiares y los profesionales sanitarios. El papel del establecimiento de buenas relaciones en la tríada profesionales-usuarios-familiares de Atención Primaria es inseparable a favor del tratamiento y preservación de la salud mental de los implicados.

Abstract Solvents play important and critical role in natural product chemistry and could generate artefacts during the extraction and purification of metabolites from a biological matrix. This study aimed to correlate the chromatographic profile with biological activity of Ipomoea pes-caprae (L.) R. Br., Convolvulaceae, extracts obtained with hydroethanolic extraction. Thus, aerial parts of I. pes-caprae were extracted with different concentration of ethanol (50, 70 and 90°GL) and the obtained extracts were analysed by HPLC–UV. HPLC data were studied employing chemometrics to discriminate the samples. Moreover these samples were further characterized by using UPLC–QTOF/MS data. The extracts were also biomonitored through the paw-oedema and spontaneous nociception induced by trypsin in mice. Different chromatographic profiles were obtained and the exploratory analysis clearly revealed higher level of ethyl caffeate in extracts of lower strength of ethanol (50°GL). This compound was suggested to be an artefact formed by transesterification of caffeoylquinic acid derivatives present in the plant, once it was not observed when other solvents were employed. During the biological assay, only the extract obtained with ethanol 50°GL presented significant inhibition of inflammation (45 ± 9%) and nociception (24 ± 3%). Ethyl caffeate seems to be linked to the anti-inflammatory effect since it reduced 86 ± 5% of paw-oedema induced by trypsin. Artefacts could contribute to the biological activity of herbal preparations and consequently lead to misinterpretation of the results.

Abstract The objective of this study was to select a population of sugarcane clones to develop energy cane cultivars. The genotypic means of 124 clones were estimated for the yield-related traits: mean number of stalks per plant (NS), mean stalk weight (MSW), fiber content (FIB), lignin content (LIG), and sucrose content in cane juice (SPC). From these data, the traits tons of cane per hectare (TCH), tons of fiber per hectare (TFH) and tons of lignin per hectare (TLH) were estimated. The clone effects were statistically significant (p <0.01) for all traits except TLH and LIG. Clone UFV09251 had the highest genotypic values for TFH and TCH. Clone UFV09270 had a high fiber content (15.04%) and clone UFV09225 a high sucrose content (14.05%). The correlation between TFH and TCH was high (0.89). Clone UFV09251 is highly promising as energy cane cultivar, for producing most TCH and FIB.

Resumen Este artículo pretende describir la interferencia de la cibernética como un factor clave para la consolidación de la información como recurso de poder en el siglo XXI. Conscientes de que la información es el principal soporte para la práctica de la inteligencia, los estudios realizados también buscan comprender las transformaciones generadas por la inclusión del ciberespacio en esta práctica, destacando cómo las características particulares de este ámbito son responsables por generar nuevas demandas a los Estados en términos de defensa y seguridad. Con el fin de lograr los objetivos propuestos, este documento se estructura en tres temas principales de discusión. La primera tendrá una breve discusión sobre las características particulares de este nuevo dominio y su significado político para las Relaciones Internacionales. El segundo tema abordará directamente las cuestiones de inteligencia. Una evaluación de la interferencia del ciberespacio en la práctica de inteligencia se llevará a cabo centrándose en el estudio de los norteamericanos - dedicando un tercer tema a dicha discusión. En términos metodológicos es un trabajo descriptivo; por lo tanto, será guiado por el análisis de los eventos internacionales que abordan este tema, así como por la literatura dedicada a tales discusiones. Este artículo no pretende terminar con tales preguntas, sino presentarse como un norte a discusiones futuras sobre este tema.

Abstract This article seeks to describe the interference of cybernetics as a key intervening factor in the consolidation of information as a power resource in the 21st century. Aware that information is the main substrate for the practice of intelligence, the studies carried out also seek to understand the transformations generated by the inclusion of cyberspace in this practice, highlighting how the particular characteristics of this domain are responsible for generating new demands to States in terms of defense and security. In order to achieve the proposed objectives, this paper is structured into three main discussion topics. The first one will hold a brief discussion about the particular characteristics of this new domain and its political significance to International Relations. The second topic will deal directly with the issues of intelligence and an evaluation of the interference of cyberspace in the intelligence practice will be carried out focusing on the study of the North American - dedicating a third topic to such discussion. In methodological terms it is a descriptive work; therefore, it will be guide by the analysis of international events that approach this subject, as well as by the literature that dedicated to such discussions. This article does not seek to end with such questions, but to present itself as a north to future discussions on this subject.

The objective of this study was to determine the deferment period and the most adequate initial sward height of Brachiaria brizantha cv. Piatã for deferment in the region of Viçosa, Minas Gerais. For so, the following characteristics were evaluated: masses and bulk density of forage and its morphological components, light interception by the sward, leaf area index, leaf angle, sward and extended tiller height and falling index of the deferred piatã grass. The three deferment periods assessed (65, 85 and 105 days) were randomized to the plots and the three sward heights (20, 30 and 40 cm), randomized in the subplots. The experimental design was of completely randomized blocks, with three replicates, in a subdivided plots arrangement. Masses of forage, green stem and dead tissue were higher in the piatã grass managed with deferment period of 105 days. The mass of green leaf blade was higher in the piatã grass deferred with 20 cm height associated to the deferment period of 85 days, which had, as consequence, greater bulk density of green leaf blade. The interception of light by the sward was greater in the piatã grass kept longer in deferment associated with the higher leaf area index, regardless of its initial height. The heights of the sward and extended tillers were greater in the piatã grass under periods of 85 and 105 days of deferment; however, the falling index was not affected by the factors studied. In the region of Viçosa, Minas Gerais, B. brizantha cv. Piatã fertilized with 75 kg/ha nitrogen and deferred with initial height of 20 cm and for a period of 85 days, from mid April, has adequate productivity and structure for animal production.

This experiment was conducted to estimate the effects of nitrogen fertilization associated with the initial height of the Piata palisade grass on the number and structural characteristics of individual tillers after the deferment period. An area with Brachiaria brizantha cv. Piata measuring approximately 288 m² was used. Combinations of levels of nitrogen fertilization (0, 75 and 150 kg/ha of nitrogen) and initial heights of the sward (low - 20 cm, medium - 30 cm and high - 40 cm) were evaluated. The experimental design was of completely randomized blocks in subdivided plots with three replications. The number of vegetative tillers, the stem length of the vegetative, reproductive and dead tillers, and the number of dead leaves of the reproductive and the dead tillers linearly increased with the elevation in the nitrogen level. The deferred Piata palisade grass with low initial height showed lower number of dead basal tillers, shorter length of stems and leaf blades in reproductive tillers and lower number of live and dead leaves in vegetative tillers in relation to the one managed with height considered medium and high. Nitrogen fertilization, in association with low initial height, provides the optimization of forage of Piata palisade grass in deferment conditions.

The objective of this study was to evaluate the mass of the forage and of the morphological components, and the sward structure in Piata palisadegrass (Brachiaria brizantha cv. Piata) deferred in function of different initial heights and nitrogen levels. Two levels of nitrogen (75 and 150 kg/ha) and one control without fertilization associated to three initial deferring heights (20, 30, and 40 cm) were evaluated. The design was of completely randomized blocks with three replications in a subdivided plot scheme. A raise in the initial deferment height and in the fertilization elevated the sward and extended tiller heights, the leaf area index, and the light interception by the sward. The piata palisadegrass deferred with an initial height of 40 cm presented a higher forage mass with lower percentage of leaf blades, and higher stem and dead tissue quantity. Additionally, this management strategy resulted in lower bulk density of leaf blade and in higher bulk densities of stems and dead tissue. The utilization of 150 kg/ha of nitrogen raised the production and the bulk density of deferred piata palisadegrass forage and increased production and bulk density of leaves and stems. In the region of Viçosa, Minas Gerais, the piata palisadegrass deferred with an initial height of 30 cm and fertilized with 75 kg/ha of nitrogen in the beginning of the fall presents adequate mass and structure to animal production in the winter.

A necessidade da introdução de novos agentes quimioterápicos é uma realidade no controle de doenças infecciosas. O Brasil é o país mais rico em biodiversidade, e o Laboratório de Extração da Universidade Paulista tem coletado plantas na Amazônia e Mata Atlântica com a finalidade de identificar extratos vegetais antibacterianos e antitumorais. Estudos prévios demonstraram que a fração etanólica obtida do extrato bruto orgânico do caule de Tabernaemontana angulata Mart. ex Müll. Arg. apresentou atividade antimicrobiana significante contra Staphylococcus aureus ATCC6538, no ensaio de diluição em caldo. Verificou-se por cromatografia em camada delgada que os compostos majoritários presentes na fração ativa eram alcalóides. No presente trabalho, foi obtida a fração de alcalóides totais a partir do extrato bruto, da qual os compostos majoritários foram isolados por sucessivas cromatografias e, posteriormente, identificados por CG-EM e ¹H-RMN como os alcalóides indólicos coronaridina e voacangina. As frações obtidas do isolamento foram testadas no ensaio biológico, porém não demonstraram atividade antimicrobiana.

Introducing new chemotherapeutic agents is a great demand in the control of infectious diseases. Brazil is one of the richest countries in biodiversity and the Laboratório de Extração at UNIP has been collecting plants from the Amazon and Atlantic Rain Forests with the aim of screening for new antibacterial and antitumor plant extracts. Previous studies demonstrated that the ethanol fraction obtained from the crude extract of Tabernaemontana angulata stems showed an antibacterial activity against Staphylococcus aureus (ATCC 6538) in the microdilution broth assay. Two alkaloids were the major compounds in the active fraction, verified by thin layer chromatography analysis. In the present study, the total alkaloids were obtained from the crude extract and were fractionated by preparative thin layer chromatography for the isolation of the main components. The isolated compounds were identified by GC/MS and ¹H-NMR as coronaridine and voacangine. The alkaloid fractions obtained from the isolation procedure were tested for antibacterial activity, but no activity was detected.

The osseointegrated titanium implants are reliable and safe alternatives to treatments for long periods of time. For surface modification, thermal aspersion of TiO2 was used. The samples with and without TiO2 were treated with NaOH and SBF in order to obtain a layer of HA. Characterization was done by SEM and FTIR. The images of HA obtained by SEM show a uniform morphology and a porous structure with spherical particles. The IR spectra show that the surface of Ticp/ TiO2 is more favorable for the HA deposit, as can be seen by the increase of the crystalline structure and the very intense and defined bands of the OH group of HA that is verified about 3571 and 630 cm-1. Thus the Ticp/ TiO2 surface presents a satisfactory nucleation of HA when compared to Ticp.

O objetivo deste estudo clínico duplo-cego, controlado, de boca-dividida foi avaliar o efeito de uma aplicação única do Gluma Desensitizer® no alívio da hipersensibilidade dentinária. Doze sujeitos participaram do estudo, sendo que destes dez completaram o estudo. Cada um dos participantes teve dois dentes tratados, um com o Gluma Desensitizer®, de acordo com as orientações do fabricante, e o outro com água. Para a avaliação da dor foi utilizada a EVA (Escala Visual Analógica), após os estímulos táctil (sonda), térmico (jato de água frio) e térmico/evaporativo (jato de ar frio) no início do experimento, imediatamente após o tratamento, uma e quatro semanas após. Os valores médios encontrados na EVA foram comparados pelo teste-t pareado (alfa = 0,05). Para os diferentes tempos experimentais utilizou-se ANOVA para medidas repetidas. Os resultados mostram que, no início do tratamento, os valores médios foram de 1,76 (± 2,82), 7,10 (± 2,10) e 4,75 (± 2,65) para os dentes teste e, após quatro semanas, os valores médios foram de 1,70 (± 2,31), 5,50 (± 3,30) e 4,61 (± 3,14) respectivamente para os estímulos sonda, água e ar. Para os dentes controle, no início do tratamento, os valores médios segundo a EVA foram de 1,86 (± 2,92), 6,61 (± 2,31) e 4,08 (± 2,91) e, após 4 semanas, 2,66 (± 3,07), 6,32 (± 2,94) e 4,76 (± 3,26). Não ocorreram diferenças estatisticamente significativas entre os dentes teste e controle em nenhum dos tempos experimentais. Também não ocorreram diferenças intragrupo. Concluímos que o Gluma Desensitizer® não tem efeito sobre a hipersensibilidade dentinária de pacientes periodontalmente tratados num período de até quatro semanas.

The aim of this double-blind, controlled, split-mouth designed clinical trial was to assess the effect of a single application of Gluma Desensitizer® on alleviating dentin hypersensitivity. Twelve subjects entered the study and ten completed the protocol. Each subject had two teeth treated: one with Gluma Desensitizer® according to the manufacturer's instructions and one with water. The assessment of pain was performed with the VAS (Visual Analogue Scale), after tactile (probe), thermal (cold blast of water) and thermal/evaporative (cold blast of air) stimuli at baseline, immediately after treatment, after 1 week and after 4 weeks. The mean VAS values for the test and control teeth were compared by the paired t test (alpha = 0.05). Repeated measurements ANOVA was used to compare the different experimental times. The results showed that for test teeth, at baseline, mean VAS values were 1.76 (± 2.82), 7.10 (± 2.10) and 4.75 (± 2.65), and, after 4 weeks, the mean values were 1.70 (± 2.31), 5.50 (± 3.30) and 4.61 (± 3.14), respectively for probe, water and air stimuli. For the control teeth, at baseline, the mean VAS values were 1.86 (± 2.92), 6.61 (± 2.31) and 4.08 (± 2.91) and, after 4 weeks, 2.66 (± 3.07), 6.32 (± 2.94) e 4.76 (± 3.26).There were no statistically significant differences between test and control teeth at any time. No intra-group differences were demonstrated either. It was concluded that Gluma Desensitizer® had no effect on hypersensitive teeth from periodontally treated patients for a period up to 4 weeks.

Commercial purity titanium (cp-Ti), and some of its alloys are important materials in the medical field because of their excellent biocompatibility and mechanical properties. Recently a simple chemical method to induce bioactivity in these inert metallic materials was reported. In this work, the biomimetic chemical process has been used to modify the surface of cp-Ti with the formation of a deposit layer of apatite (a calcium phosphate compound). The main purpose was to study the influence of heat treatment on changes in crystallinity in the deposited phases. X-ray diffraction analysis and scanning electron microscopy showed that the apatite coatings heat treated between 400 and 600 °C were less crystalline, similar to biological apatites. Upon heat treatment at temperatures above 700 °C, the apatite coatings appeared more crystalline, and were a mixture of hydroxyapatite, octacalcium phosphate and magnesium phosphate.