High-altitude hypoxia exposure can lead to phospholipase D-mediated lipid metabolism disorder in spleen tissues and induce ferroptosis. Nonetheless, the key genes underlying hypoxia-induced splenic phospholipase D and the ferroptosis pathway remain unclear. This study aimed to establish a hypoxia animal model. Combined transcriptomic and proteomic analyses showed that 95 predicted target genes (proteins) were significantly differentially expressed under hypoxic conditions. Key genes in phospholipase D and ferroptosis pathways under hypoxic exposure were identified by combining Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis techniques. Gene set enrichment analysis (GSEA) showed that the differential gene sets of the phospholipase D and ferroptosis signaling pathways were upregulated in the high-altitude hypoxia group. The genes in the phospholipase D signalling pathway were verified, and the expression levels of KIT and DGKG were upregulated in spleen tissues under hypoxic exposure. Subsequently, the mRNA and protein expression levels of genes from the exogenous pathway such as TFRC, SLC40A1, SLC7A11, TRP53, and FTH1 and those from the endogenous pathway such as GPX4, HMOX1, and ALOX15 differentials in the ferroptosis signalling pathway were verified, and the results indicated significant differential expression. In summary, exposure to high-altitude hypoxia mediated phospholipid metabolism disturbance through the phospholipase D signalling pathway and further induced ferroptosis, leading to splenic injury. Highaltitude High altitude Dmediated Nonetheless hypoxiainduced unclear model 9 proteins (proteins conditions GO (GO KEGG (KEGG techniques GSEA (GSEA highaltitude high group verified Subsequently TFRC SLC40A1 SLCA SLC A SLC7A11 TRP53 TRP FTH GPX4 GPX HMOX1 HMOX ALOX ALOX1 summary injury SLC40A SLC7A1 TRP5 SLC7A

Abstract Objectives To investigate the impact of Three-Dimensional (3D) laparoscopy compared to traditional laparotomy on serum tumor markers and coagulation function in patients diagnosed with early-stage Endometrial Cancer (EC). Method The authors retrospectively analyzed the clinical data of 75 patients diagnosed with early-stage EC and categorized them into two groups based on the surgical techniques employed. The 3D group consisted of 36 patients who underwent 3D laparoscopic surgery, while the Laparotomy group comprised 39 patients who underwent traditional laparotomy. The authors then compared the alterations in serum tumor markers and coagulation function between the two groups. Results Postoperatively, serum levels of CA125, CA199, and HE4 were notably reduced in both groups on the third day, with the levels being more diminished in the 3D group than in the Laparotomy Group (p < 0.05). Conversely, FIB levels escalated significantly in both groups on the third-day post-surgery, with a more pronounced increase in the 3D group. Additionally, PT and APTT durations were reduced and were more so in the 3D group than in the laparotomy group (p < 0.05). Conclusions When juxtaposed with traditional laparotomy, 3D laparoscopic surgery for early-stage EC appears to be more efficacious, characterized by reduced complications, and expedited recovery. It can effectively mitigate serum tumor marker levels, attenuate the inflammatory response and damage to immune function, foster urinary function recovery, and enhance the quality of life. However, it exerts a more significant influence on the patient's coagulation parameters, necessitating meticulous prevention and treatment strategies for thromboembolic events in clinical settings. ThreeDimensional Three Dimensional D (3D earlystage early stage EC. . (EC) 7 employed 3 Postoperatively CA125 CA CA199 HE day p 0.05. 005 0.05 0 05 0.05) Conversely thirdday postsurgery, postsurgery post post-surgery Additionally efficacious complications recovery life However patient s parameters settings (EC CA12 CA19 00 0.0 CA1 0.

Coenzyme Q10 (CoQ10) is a potent antioxidant that is implicated in the inhibition of osteoclastogenesis, but the underlying mechanism has not been determined. We explored the underlying molecular mechanisms involved in this process. RAW264.7 cells received receptor activator of NF-κB ligand (RANKL) and CoQ10, after which the differentiation and viability of osteoclasts were assessed. After the cells were treated with CoQ10 and/or H2O2 and RANKL, the levels of reactive oxygen species (ROS) and proteins involved in the PI3K/AKT/mTOR and MAPK pathways and autophagy were tested. Moreover, after the cells were pretreated with or without inhibitors of the two pathways or with the mitophagy agonist, the levels of autophagy-related proteins and osteoclast markers were measured. CoQ10 significantly decreased the number of TRAP-positive cells and the level of ROS but had no significant impact on cell viability. The relative phosphorylation levels of PI3K, AKT, mTOR, ERK, and p38 were significantly reduced, but the levels of FOXO3/LC3/Beclin1 were significantly augmented. Moreover, the levels of FOXO3/LC3/Beclin1 were significantly increased by the inhibitors and mitophagy agonist, while the levels of osteoclast markers showed the opposite results. Our data showed that CoQ10 prevented RANKL-induced osteoclastogenesis by promoting autophagy via inactivation of the PI3K/AKT/mTOR and MAPK pathways in RAW264.7 cells. Q Q1 CoQ (CoQ10 determined process RAW2647 RAW RAW264 7 RAW264. NFκB NF κB RANKL (RANKL assessed CoQ1 andor HO H O H2O (ROS PI3KAKTmTOR PIKAKTmTOR PI3K AKT mTOR PI K tested Moreover agonist autophagyrelated related measured TRAPpositive TRAP positive PIK ERK p p3 reduced FOXO3LC3Beclin1 FOXOLCBeclin FOXO3 LC3 Beclin1 FOXO LC Beclin FOXO3/LC3/Beclin augmented results RANKLinduced induced (CoQ1 RAW26 KAKTmTOR FOXO3LC3Beclin (CoQ RAW2

ABSTRACT This study aimed to systematically review interventions to prevent mother-to-child transmission of HIV during breastfeeding. We conducted a systematic review and meta-analysis using specific criteria to identify randomized controlled trials that focused on pregnant and breastfeeding women living with HIV and their children from birth to 2 years of age. We extensively searched electronic databases, including Web of Science, Scopus, PubMed, MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, and Google Scholar up to October 24, 2023. After screening 3,110 titles and abstracts, we reviewed 306 full texts. Of these, we assessed the quality and risk of bias of fifty-five articles, ultimately identifying seven studies. Four of these studies, which focused on antiretroviral therapy (ART), were included in the meta-analysis. There was little heterogeneity in study methodology and pooled estimates. The postnatal HIV transmission rate was found to be 0.01 (95%CI: 0.00 – 0.02). Therefore, the risk of mother-to-child transmission among breastfeeding mothers with HIV was significantly lower in the intervention groups than in the placebo groups. Analysis of funnel plots and Egger’s test (p = 0.589) showed no evidence of publication bias. In addition to the four articles, two studies compared different ART regimens and one study compared the administration of high-dose vitamin A to the mother or the child. The results suggest that the use of ART significantly reduces the risk of postnatal HIV transmission compared with placebo. However, the effectiveness of different ART regimens or other therapies, including high-dose vitamin A, is unclear. mothertochild child metaanalysis meta analysis age databases Science Scopus PubMed MEDLINE EMBASE Trials 24 2023 3110 3 110 3,11 abstracts 30 texts fiftyfive fifty five articles ART, , (ART) metaanalysis. analysis. estimates 001 0 01 0.0 95%CI 95CI CI 95 (95%CI 000 00 0.02. 002 0.02 . 02 0.02) Therefore Eggers Egger s p 0.589 0589 589 highdose high dose However therapies unclear 202 311 11 3,1 (ART 0. 9 0.58 058 58 20 31 1 3, 0.5 05 5

Abstract Objective To explore the value of serum Dickkopf-3 (sDKK3) in predicting Early Neurological Deterioration (END) and in-hospital adverse outcomes in acute ischemic stroke (AIS) patients. Methods AIS patients (n = 200) were included and assessed by the National Institutes of Health Stroke Rating Scale. Serum Dkk3 levels were assessed by ELISA. END was defined as an increase of ≥ 4 points in NIHSS score within 72h. The biological threshold of sDKK3 level and END occurrence were predicted based on X-tile software. Primary outcomes were END and all-cause death, and the secondary outcome was ICU admission during hospitalization. The logistic regression model and Cox risk regression model were applied to evaluate the relationship between DKK3 level and END incidence, all-cause in-hospital mortality, and in-hospital adverse outcomes (ICU admission). Results During hospitalization, the incidence of END in patients with AIS was 13.0 %, and the mortality rate within 7 days after END was 11.54 % (3/26). In patients below the serum DKK3 cutoff (93.0 pg/mL), the incidence of END was 43.5 % (20/48). Patients with lower sDKK3 levels were associated with a 1.188-fold increased risk of developing END (OR = 1.188, 95 % CI 1.055‒1.369, p < 0.0001). However, there was no significant association with admission to the ICU. sDKK3 below the threshold (93.0 pg/mL) was a risk factor for death. Conclusion Predictive threshold levels of serum DKK3 based on X-tile software may be a potential predictive biomarker of in-hospital END in patients with AIS, and low levels of DKK3 are independently associated with increased in-hospital mortality. Dickkopf3 Dickkopf 3 Dickkopf- sDKK (sDKK3 (END inhospital hospital (AIS n 200 Scale Dkk ELISA 72h h Xtile X tile allcause all cause death hospitalization DKK admission. . admission) 130 13 0 13. 1154 11 54 11.5 3/26. 326 3/26 26 (3/26) 93.0 930 93 (93. pg/mL, pgmL pg/mL , pg mL 435 43 5 43. 20/48. 2048 20/48 20 48 (20/48) 1.188fold 1188fold fold 1.188 1 188 OR 1188 9 10551369 055 369 1.055‒1.369 0.0001. 00001 0.0001 0001 0.0001) However (sDKK 115 11. 32 3/2 2 (3/26 93. (93 204 20/4 (20/48 188fold 1.18 18 118 1055136 05 36 1.055‒1.36 0000 0.000 000 3/ (3/2 (9 20/ (20/4 1.1 105513 1.055‒1.3 0.00 00 (3/ ( (20/ 1. 10551 1.055‒1. 0.0 (3 (20 1055 1.055‒1 0. (2 105 1.055‒ 10 1.055 1.05 1.0

In this study, Al-Si-Cu-Mg composites reinforced with Silicon carbide (SiC) particles and/or Carbon nanotubes (CNTs) were fabricated using semi-solid stirring casting technology (SSC). The aim of the study was to investigate the influence of SiC and/or CNTs on the mechanical properties, microstructural evolution, and deformation mechanisms of the Al-Si-Cu-Mg alloy. The findings indicated that the presence of SiC/CNTs in the composite had a significant effect on refining the α-Al phase and altering its grain orientation. Additionally, the results obtained from EBSD and TEM revealed that the microstructure of the Al-Si-Cu-Mg-SiC/CNTs hybrid composite (HAMC) underwent dynamic recrystallization (DRX) and static recrystallization (SRX), resulting in a fine equiaxed recrystallized structure. This process also led to the formation of high-angle grain boundaries (HAGBs) through dislocation rearrangement. The SiC particles were evenly distributed within the matrix, ensuring good interface bonding between SiC and α-Al phases. Moreover, the CNTs reacted with the matrix, resulting in the in-situ formation of the Al4C3 phase at the interface. The addition of SiC/CNTs significantly increased the tensile strength of HAMC at 25°C and 250°C, from 319MPa and 178MPa to 438MPa and 331MPa. Examination of the fracture surface of the hybrid composite unveiled that void formation occurred primarily at the regions surrounding the matrix-particle interface. AlSiCuMg Al Si Cu Mg (SiC andor or (CNTs semisolid semi solid SSC. SSC . (SSC) properties evolution alloy SiCCNTs αAl α orientation Additionally AlSiCuMgSiC/CNTs AlSiCuMgSiCCNTs (HAMC DRX (DRX SRX, SRX , (SRX) structure highangle high angle HAGBs (HAGBs rearrangement matrix phases Moreover insitu situ AlC C Al4C 25C 25 250C 250 250°C MPa 331MPa matrixparticle particle (SSC AlSiCuMgSiC (SRX 2

RESUMO Objetivo O objetivo deste estudo foi investigar o efeito da administração oral de proteína do soro de leite hidrolisada durante o período perioperatório da cesariana nos níveis de prolactina pós-operatória e no leite materno das mães. Métodos Este estudo randomizado triplo-cego foi conduzido em mulheres que deram à luz em um hospital chinês de cuidados terciários. As participantes foram aleatoriamente designadas para o grupo de proteína do soro de leite hidrolisada ou para o grupo placebo e receberam as respectivas substâncias em momentos específicos durante o período perioperatório. Os resultados primários foram os níveis de prolactina e a ingestão adicional de leite fluido durante períodos pós-operatórios específicos. As observações secundárias incluíram os sentimentos subjetivos das mães no pré-operatório e o tempo de exaustão no pós-operatório. Resultados As mães do grupo de proteína do soro de leite hidrolisada apresentaram menos fome visual (p=0,002) e ansiedade (p<0,001), enquanto seus recém-nascidos tinham níveis mais altos de glicose no sangue (p=0,041). As mães do grupo da proteína hidrolisada do soro de leite apresentaram níveis mais altos de prolactina (p=0,009) e seus recém-nascidos tiveram menos reabastecimento de leite nas primeiras 48 horas de vida (p=0,035). Conclusão A ingestão de proteína do soro de leite hidrolisada durante o período perioperatório da cesariana reduz a fome e a ansiedade maternas, aumenta os níveis séricos de prolactina 3-4 horas após a cirurgia, aumenta o nível de glicose no sangue do bebê 30 minutos após o nascimento e reduz a suplementação de leite em 48 horas. pósoperatória pós operatória triplocego triplo cego terciários pósoperatórios operatórios préoperatório pré operatório pósoperatório. pósoperatório operatório. pós-operatório p=0,002 p0002 p 0 002 (p=0,002 p<0,001, p0001 p<0,001 , 001 (p<0,001) recémnascidos recém nascidos p=0,041. p0041 p=0,041 . 041 (p=0,041) p=0,009 p0009 009 (p=0,009 4 p=0,035. p0035 p=0,035 035 (p=0,035) maternas 34 3 3- cirurgia p=0,00 p000 00 (p=0,00 p<0,00 (p<0,001 p004 p=0,04 04 (p=0,041 p003 p=0,03 03 (p=0,035 p=0,0 p00 (p=0,0 p<0,0 (p<0,00 (p=0,04 (p=0,03 p=0, p0 (p=0, p<0, (p<0,0 p=0 (p=0 p<0 (p<0, p= (p= p< (p<0 (p (p<

ABSTRACT Objective This study aimed to investigate the effect of oral administration of hydrolyzed whey protein during the perioperative period of cesarean section on postoperative prolactin levels and breast milk production in mothers. Methods triple-blind randomized trial was conducted on women who gave birth in a Chinese tertiary care hospital. Participants were randomly assigned to either the hydrolyzed whey protein or placebo group and were administered the respective substances at specific times during the perioperative period. The primary outcomes were prolactin levels and additional fluid milk intake during specific postoperative periods. Secondary observations included mothers’ subjective preoperative feelings and postoperative exhaust time. Results The study found that mothers in the hydrolyzed whey protein group had reduced visual hunger (p=0.002) and anxiety (p<0.001) while having higher neonatal blood glucose levels (p=0.041) compared to the placebo group. This group also had higher prolactin levels (p=0.009) and a reduced the number of milk additions in the first 48 hours of a newborn’s life (No-BreakpNo-Break=0.035). Conclusion Hydrolyzed whey protein intake during the cesarean section perioperative period reduces maternal hunger and anxiety, increases serum prolactin levels 3-4 hours after surgery, increases the infant’s blood glucose level 30 minutes after birth, and reduces milk supplementation in the first 48 hours. tripleblind triple blind hospital periods time p=0.002 p0002 p 0 002 (p=0.002 p<0.001 p0001 001 (p<0.001 p=0.041 p0041 041 (p=0.041 p=0.009 p0009 009 (p=0.009 4 newborns newborn s NoBreakpNoBreak=0.035. NoBreakpNoBreak0035 NoBreakpNoBreak No BreakpNo Break=0.035 . Break 035 (No-BreakpNo-Break=0.035) 34 3 3- surgery infants infant p=0.00 p000 00 (p=0.00 p<0.00 (p<0.00 p=0.04 p004 04 (p=0.04 NoBreakpNoBreak=0.035 NoBreakpNoBreak003 Break0035 Break=0.03 03 (No-BreakpNo-Break=0.035 p=0.0 p00 (p=0.0 p<0.0 (p<0.0 NoBreakpNoBreak=0.03 NoBreakpNoBreak00 Break003 Break=0.0 (No-BreakpNo-Break=0.03 p=0. p0 (p=0. p<0. (p<0. NoBreakpNoBreak=0.0 NoBreakpNoBreak0 Break00 Break=0. (No-BreakpNo-Break=0.0 p=0 (p=0 p<0 (p<0 NoBreakpNoBreak=0. Break0 Break=0 (No-BreakpNo-Break=0. p= (p= p< (p< NoBreakpNoBreak=0 Break= (No-BreakpNo-Break=0 (p NoBreakpNoBreak= (No-BreakpNo-Break= (No-BreakpNo-Break

Objective: Panic disorder (PD) is a common disabling condition characterized by recurrent panic attacks. Emotional and behavioral impairments are associated with functional connectivity (FC) and network abnormalities. We used whole-brain FC, modular networks, and graph-theory analysis to investigate extensive network profiles in PD. Methods: Functional magnetic resonance imaging (fMRI) data from 82 subjects with PD and 97 healthy controls were included. Intrinsic FC between each pair of 160 regions, six intra-network, and 15 inter-network FCs were analyzed. Topological properties were explored. Results: PD patients showed altered FCs within the right insula, between frontal cortex-posterior cingulate cortex (PCC), frontal cortex-cerebellum, and PCC-occipital cortex (corrected p < 0.001). Lower connections within the sensorimotor network (SMN) and SMN-occipital network (OCN) were detected (p < 0.05). Various decreased global and local network features were found in PD (p < 0.05). In addition, significant correlations were found between PD symptoms and nodal efficiency (Ne) in the insula (r = -0.273, p = 0.016) and intra-insula FC (r = -0.226, p = 0.041). Conclusion: PD patients present with abnormal functional brain networks, especially decreased FC and Ne within the insula, suggesting that dysfunction of information integration plays an important role in PD. Objective (PD attacks (FC abnormalities wholebrain whole networks graphtheory graph theory Methods fMRI (fMRI 8 9 included 16 regions intranetwork, intranetwork intra network, intra-network 1 internetwork inter analyzed explored Results cortexposterior posterior PCC, PCC , (PCC) cortexcerebellum, cortexcerebellum cerebellum, cerebellum cortex-cerebellum PCCoccipital occipital corrected 0.001. 0001 0.001 . 0 001 0.001) SMN (SMN SMNoccipital OCN (OCN 0.05. 005 0.05 05 0.05) addition (Ne r 0.273, 0273 273 -0.273 0.016 0016 016 intrainsula 0.226, 0226 226 -0.226 0.041. 0041 0.041 041 0.041) Conclusion (PCC 000 0.00 00 0.0 0.273 027 27 -0.27 0.01 01 0.226 022 22 -0.22 004 0.04 04 0. 0.27 02 2 -0.2 0.22 0.2 -0. -0 -

Abstract Background and purpose: the Dietary Approaches to Stop Hypertension (DASH) diet has multifunctional health benefits. We evaluated the effects of low-sodium salt applied to Chinese modified DASH diet on arterial stiffness in older patients with hypertension and type 2 diabetes. Methods: sixty-one older adults with hypertension and type 2 diabetes were randomly allocated to low sodium salt group (n = 31) or normal sodium salt group (n = 30). They were given the Chinese modified DASH diet plus low-sodium salt (52 % sodium chloride) or same diet plus regular salt (99 % sodium chloride) for eight weeks, respectively. Brachial and ankle pulse wave conduction velocity, ankle brachial index and atherosclerosis-related indices were measured at baseline and week 8. In addition, 24-hour urine and blood samples were measured at baseline, the 4th week and the end of the intervention. Results: as compared with the baseline, the low sodium salt group significantly decreased in ankle brachial index (-0.09 ± 0.11, p < 0.001) and brachial and ankle pulse wave conduction velocity (-133.07 ± 265.99 cm/s, p = 0.010) at week 8 while the normal sodium salt group only decreased significantly in ankle brachial index (-0.06 ± 0.12, p = 0.010) at week 8. Conclusion: the low-sodium salt applied to Chinese modified DASH diet may improve arterial stiffness in patients with hypertension and type 2 diabetes. Further research with an extended follow-up is needed.

Resumen Antecedentes y propósito: la dieta DASH (del inglés Dietary Approaches to Stop Hypertension) tiene beneficios multifuncionales para la salud. Evaluamos los efectos de una sal baja en sodio aplicada a la versión modificada china de la dieta DASH sobre la rigidez arterial en pacientes mayores con hipertensión y diabetes tipo 2. Métodos: sesenta y un adultos mayores con hipertensión arterial y diabetes tipo 2 fueron asignados al azar al grupo de sal baja en sodio (n = 31) o al grupo de sal con contenido normal de sodio (n = 30). Se les administró la versión china modificada de la dieta DASH más sal baja en sodio (52 % de cloruro de sodio) o la misma dieta más sal con la cantidad normal de sodio (99 % cloruro de sodio) durante ocho semanas, respectivamente. Se midieron la velocidad de onda de pulso brazo-tobillo, el índice brazo-tobillo y los índices relacionados con la aterosclerosis al inicio del estudio y a la semana 8. Se recogieron muestras de orina y sangre de 24 horas al inicio, a la cuarta semana y al final de la intervención. Resultados: en comparación con el estado inicial, el grupo de sal baja en sodio disminuyó significativamente el índice brazo-tobillo (-0,09 ± 0,11, p < 0,001) y la velocidad de onda de pulso brazo-tobillo (-133,07 ± 265,99 cm/s, p = 0,010) en la semana 8, mientras que el grupo de sal con contenido normal de sodio solo disminuyó significativamente en el índice brazo-tobillo (-0,06 ± 0,12, p = 0,010) en la semana 8. Conclusión: la sal baja en sodio aplicada a la versión china modificada de la dieta DASH puede mejorar la rigidez arterial en pacientes con hipertensión y diabetes tipo 2. Se necesitan más investigaciones con un seguimiento prolongado.

Abstract Aim: to investigate the effects of low sodium formula salt combined with the Chinese Modified Dietary Approaches to Stop Hypertension (DASH) diet on cerebrovascular function in patients with hypertension and type 2 diabetes. Methods: an eight-week single-arm trial was conducted in 66 patients with hypertension and type 2 diabetes to investigate effects of low sodium formula salt (potassium chloride 56 %, sodium chloride 23 %, 5 g/day) combined with Chinese Modified DASH diet on cerebrovascular function (measured by transcranial Doppler sonography), indicators of chronic diseases (blood pressure, blood glucose and blood lipids) and urinary excretion. The above indicators were performed before and after intervention. Results: fifty-nine subjects completed the study. Peak systolic velocity, mean flow velocity, end-diastolic velocity, pulsatility index and resistance index of internal cerebral artery and vertebral artery decreased significantly (p < 0.05); pulsatility index and resistance index of anterior cerebral artery and middle cerebral artery decreased significantly (p < 0.05); and end-diastolic velocity and pulsatility index of basilar artery decreased significantly (p < 0.05). Systolic blood pressure, diastolic blood pressure, fasting blood glucose and postprandial blood glucose decreased significantly (p < 0.001; p < 0.001; p < 0.001; p < 0.001). Blood pressure and blood glucose control rates increased significantly (p < 0.001). Conclusions: based on the study, 23 % low-sodium formula combined with CM-DASH diet pattern can improve cerebrovascular function in community patients with hypertension complicated with diabetes and has a good short-term benefit of blood pressure and glucose control.

Resumen Objetivo: investigar los efectos de una sal baja en sodio combinada con la versión china de la dieta DASH (del inglés Dietary Approaches to Stop Hypertension) modificada en la función cerebrovascular en pacientes con hipertensión y diabetes tipo 2. Métodos: en 66 pacientes con hipertensión y diabetes tipo 2 se llevó a cabo un ensayo de ocho semanas para investigar los efectos de la sal baja en sodio (cloruro de potasio 56 %, cloruro de sodio 23 %, 5 g/día) combinada con la dieta DASH en su versión china modificada en la función cerebrovascular (medido por sonografía Doppler transcraneal), los indicadores de enfermedades crónicas (presión arterial, glucosa sanguínea y lípidos sanguíneos) y la excreción urinaria. Los indicadores anteriores se midieron antes y después de la intervención. Resultados: cincuenta y nueve sujetos completaron el estudio. La velocidad sistólica máxima, la velocidad media del flujo, la velocidad diastólica final, el índice de pulsatilidad y el índice de resistencia de la arteria cerebral anterior y la arteria vertebral disminuyeron significativamente (p < 0,05); el índice de pulsatilidad y el índice de resistencia de la arteria cerebral anterior y la arteria media disminuyeron significativamente (p < 0,05); y la velocidad diastólica final y el índice de pulsatilidad de la arteria basilar disminuyeron significativamente (p < 0,05). La presión arterial sistémica, la presión arterial diastólica, la glucosa arterial en ayuno y la glucemia posprandial disminuyeron significativamente (p < 0,001, p < 0,001, p < 0,001, p < 0,001). La presión arterial y las tasas de control de glucosa en sangre aumentaron significativamente (p < 0,001). Conclusiones: la fórmula de sal con un 23 % de sodio combinada con la dieta DASH en su versión china modificada puede mejorar la función cerebrovascular en pacientes comunitarios con hipertensión complicada por la diabetes y es beneficiosa a corto plazo para la presión arterial y el control de la glucosa.

Abstract Anthocyanin/polyvinyl alcohol/chitosan composite membrane was prepared by solution casting method using chitosan (CS) and polyvinyl alcohol (PVA) as matrix and natural pigment anthocyanin as filler. The structures of the composite films were characterized by Uv-visible reflectance spectrum (UV-VIS), Fourier infrared spectroscopy (FT-IR) and the scanning electron microscopy (SEM). The effects of adding anthocyanins on the physical properties, water resistance, degradation, antibacterial properties and pH responsiveness of the films were investigated. The experimental results showed that the prepared anthocyanin/polyvinyl alcohol/chitosan composite indicator films show color changes from pink to purple to blue and then to yellow-green when the pH value ranges from 2 to 14. The total color difference ΔE value is greater than 12. The antibacterial properties and other properties of the composite film can enhance by adding anthocyanin. The tensile strength of anthocyanin/polyvinyl alcohol/chitosan film is more than 70 MPa, which is larger than that of polyvinyl film and polyvinyl alcohol/chitosanfilm. The film has excellent physical, antibacterial properties and obvious pH response performance, making it ideal for food packaging.

Although non-invasive prenatal testing (NIPT) is widely used to detect fetal abnormalities, the results of NIPT vary by population, and data for the screening efficiency of NIPT positive predictive value (PPV) from different populations is limited. Herein, we retrospectively analyzed the NIPT results in a large multicenter study involving 52,855 pregnant women. Depending on gestational age, amniotic fluid or umbilical cord blood was extracted for karyotype and/or chromosome microarray analysis (CMA) in NIPT-positive patients, and the PPV and follow-up data were evaluated to determine its clinical value. Among the 52,855 cases, 754 were NIPT-positive, with a positivity rate of 1.4%. Karyotype analysis and/or CMA confirmed 323 chromosomal abnormalities, with a PPV of 45.1%. PPV for trisomy 21 (T21), trisomy 18 (T18), trisomy 13 (T13), sex chromosomal aneuploidies (SCAs), and copy number variations (CNVs) were 78.9, 35.3, 22.2, 36.9, and 32.9%, respectively. The PPVs for T21, T18, and T13 increased with age, whereas the PPVs for SCAs and CNVs had little correlation with age. The PPV was significantly higher in patients with advanced age and abnormal ultrasound. The NIPT results are affected by population characteristics. NIPT had a high PPV for T21 and a low PPV for T13 and T18, and screening for SCAs and CNVs showed clinical significance in southern China. noninvasive non invasive (NIPT abnormalities (PPV limited Herein 52855 52 855 52,85 women andor (CMA NIPTpositive followup follow up cases 75 NIPTpositive, positive, 14 1 4 1.4% 32 451 45 45.1% 2 T , (T21) T18 (T18) T13, (T13) SCAs, (SCAs) (CNVs 789 78 9 78.9 353 35 3 35.3 222 22 22.2 369 36 36.9 329 32.9% respectively T1 ultrasound characteristics T2 China 5285 5 85 52,8 7 1.4 45.1 (T21 (T18 (T13 (SCAs 78. 35. 22. 36. 32.9 528 8 52, 1. 45. (T2 (T1 32. (T

Abstract Objective To determine prevalence and factors associated with flares post Coronavirus disease 2019 (COVID-19) mRNA vaccination in patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA) and spondyloarthritis (SpA). Methods A retrospective multi-centre study was conducted (January 2021 to February 2022). Data were collected during index visit, defined as first post-vaccine visit in which the patient had a physician-defined flare, or if at least 3 months had elapsed since first vaccine dose, whichever came first. Factors associated with flares were identified using mixed effects Cox regression and expressed as hazard ratio (HR) and 95% confidence interval (CI). Results Total of 2377 patients were included (1563 RA, 415 PsA and 399 SpA). Among patients with RA, PsA and SpA, 21.3%, 24.1% and 21.8% experienced a flare respectively. Of those who experienced a flare, only 10.2%, 11.0% and 14.9% were severe in patients with RA, PsA and SpA respectively. Patients with low or moderate/high disease were more likely to flare compared to those in remission in patients with RA only (HR: 1.68, 95% CI 1.22–2.31; HR: 2.28, 95% CI 1.50–3.48, respectively). Receiving the Moderna vaccine was associated with a higher HR of flare compared to the Pfizer vaccine in patients with PsA only (HR: 2.21, 95% CI 1.20–4.08). Patients who had two vaccine doses were found to be less likely to flare (HR: 0.08, 95% CI 0.06–0.10). HRs of flares were not significantly different among RA, PsA and SpA. Conclusion About one-fifth of patients experienced a disease flare post COVID-19 mRNA vaccination, but most flares were non-severe. Patients with active disease prior to vaccination should be monitored closely for disease flares, especially in patients with RA. 201 COVID19 COVID 19 (COVID-19 , (RA) (PsA . (SpA) multicentre multi centre January 202 2022. 2022 2022) postvaccine physiciandefined physician dose (HR 95 CI. (CI) 237 1563 (156 41 39 SpA) 213 21 21.3% 241 24 1 24.1 218 8 21.8 respectively 102 10 2 10.2% 110 11 0 11.0 149 14 9 14.9 moderatehigh moderate high 168 68 1.68 1.22–2.31 122231 22 31 228 28 2.28 150348 50 48 1.50–3.48 respectively) 221 2.21 1.20–4.08. 120408 1.20–4.08 20 4 08 1.20–4.08) 008 0.08 0.06–0.10. 006010 0.06–0.10 06 0.06–0.10) onefifth one fifth COVID-1 nonsevere. nonsevere non severe. non-severe COVID1 (COVID-1 (RA (SpA (CI 23 156 (15 21.3 24. 21. 10.2 11. 14. 16 6 1.6 1.22–2.3 12223 2.2 15034 5 1.50–3.4 12040 1.20–4.0 00 0.0 00601 0.06–0.1 COVID- (COVID- 15 (1 10. 1. 1.22–2. 1222 2. 1503 1.50–3. 1204 1.20–4. 0. 0060 0.06–0. (COVID ( 1.22–2 122 150 1.50–3 120 1.20–4 006 0.06–0 1.22– 12 1.50– 1.20– 0.06– 1.22 1.50 1.20 0.06 1.2 1.5

Abstract Soybean are rich in functional nutrients such as protein, Essential amino acid, Polyunsaturated fatty acid, minerals, vitamins and dietary fibre which can help maintain optimal body weight, prevent Alzheimer and cardiovascular disease. At the same time, it also contains some anti-nutritional factors which have adverse effects on the digestion, absorption and utilization of nutrients. Cellular and metabolic events are induced by water absorption during seed germination. After absorbing water, soybean dry seeds begin to mobilize organelles and enzyme activities, repair and activate physiological and biochemical processes, and start basic metabolism. After that, by controlling the key factors of metabolism, the cells complete the process of material transformation, energy metabolism, ROS balance, cell wall acidification and looseness, hypocotyl elongation, etc. and finish germination. As a result, cell metabolism causes significant changes in proteins, lipids, sugars, vitamins and minerals, and increases in some beneficial functional nutritional factors for humans and animals, the other anti-nutritional factors decreased obviously, which greatly improved the taste quality and economic value of soybean. This paper introduced the germination physiology, functional nutrients and their metabolic pathways during soybean germination. This study has important reference value for the study of theory and practice technology of soybean germination. protein acid minerals weight disease time antinutritional anti digestion activities processes that transformation balance looseness elongation etc result proteins lipids sugars animals obviously physiology

Inadequate invasion and excessive apoptosis of trophoblast cells are associated with the development of preeclampsia. Vitamin D deficiency in pregnant women may lead to an increased risk of preeclampsia. However, the underlying mechanisms by which vitamin D is effective in preventing preeclampsia are not fully understood. The objectives of this study were to investigate the role of lysosome-associated membrane glycoprotein 3 (LAMP3) in the pathogenesis of preeclampsia and to evaluate whether vitamin D supplementation would protect against the development of preeclampsia by regulating LAMP3 expression. Firstly, the mRNA and protein levels of LAMP3 were significantly upregulated in the placentas of preeclampsia patients compared to normal placentas, especially in trophoblast cells (a key component of the human placenta). In the hypoxia/reoxygenation (H/R)-exposed HTR-8/Svneo trophoblast cells, LAMP3 expression was also upregulated. H/R exposure repressed cell viability and invasion and increased apoptosis of trophoblast cells. siRNA-mediated knockdown of LAMP3 increased cell viability and invasion and suppressed apoptosis of H/R-exposed trophoblast cells. We further found that 1,25(OH)2D3 (the hormonally active form of vitamin D) treatment reduced LAMP3 expression in H/R exposed trophoblast cells. In addition, 1,25(OH)2D3 treatment promoted cell viability and invasion and inhibited apoptosis of H/R-exposed trophoblast cells. Notably, overexpression of LAMP3 abrogated the protective effect of 1,25(OH)2D3 on H/R-exposed trophoblast cells. Collectively, we demonstrated trophoblast cytoprotection by vitamin D, a process mediated via LAMP3. However understood lysosomeassociated lysosome LAMP (LAMP3 Firstly placenta. placenta . placenta) hypoxiareoxygenation hypoxia reoxygenation H/Rexposed HRexposed H R HTR8/Svneo HTR8Svneo HTRSvneo HTR 8/Svneo 8 Svneo HR siRNAmediated siRNA 1,25OH2D3 125OH2D3 OHD 1,25 OH 2D3 1 25 1,25(OH)2D addition Notably Collectively (LAMP Rexposed HTR8 8Svneo 25OH2D3 1,25OH2D 125OH2D 125 1,2 2D 2 25OH2D 12 1,