ABSTRACT Background. The Veracruz coastal zone in Mexico has a vast coastline where different tourist (use of beaches), economic (urban-port, commercial, and gastronomic), social, cultural, and sports activities are carried out. In Mexico, the only regulation that refers to the quality of beaches is NMX-AA-120-SCFI-2016, in which the absence of fecal matter is determined as an indicator of its sanitary quality. However, the inadequate management of fecal material in recreational beaches compromises public health and contributes to water and sand quality deterioration. Objective. To evaluate the presence of parasitic forms of zoonotic importance in the sand of the beaches of the Veracruz-Boca del Río metropolitan area that affect the Veracruz Reef System National Park. Methods. A total of 100 samples of beach sand and 19 samples of fecal matter were taken in the period from June to October 2021. The analyzes were carried out at the Boca del Río Technological Institute/Aquatic Resources Research Laboratory by the Willis flotation technique and cup sedimentation. The presence of helminths was identified and quantified as bioindicators of fecal matter contamination. Results. Of the sand samples, 28 % were positive for parasitic forms with a recovery of 112 parasitic forms, of which 67.85 % correspond to parasites of the genus Strongyloides sp. and Hookworms. 32.35 % corresponds to non-pathogenic parasites for humans. Of the fecal matter samples collected, only 42.1 % showed positivity to the parasitized forms mentioned above. Conclusions. The presence of parasitic pathogenic forms in the sand samples indicates fecal contamination, which compromises the sanitary and environmental quality of the beach sand of the Veracruz-Boca del Río metropolitan area and the Veracruz Reef System National Park.

RESUMEN Antecedentes. La zona costera de Veracruz cuenta con un amplio litoral en donde se realizan diferentes actividades turísticas (uso de playas), económicas (urbano-portuario, comercial y gastronómico), sociales, culturales y deportivas. En México, la única norma que hace referencia a la calidad de playas es la NMX-AA-120-SCFI-2016, en la cual se determina la ausencia de materia fecal como indicador de su calidad sanitaria; sin embargo, el inadecuado manejo de la materia fecal canina en playas recreativas compromete la salud pública y contribuye al deterioro de la calidad del agua y arena. Objetivo. Evaluar la presencia de formas parasitarias de importancia zoonótica en arena de playas de la zona conurbada Veracruz-Boca del Río que inciden en el Parque Nacional Sistema Arrecifal Veracruzano. Métodos. Se tomaron en total 100 muestras de arena de playa y 19 muestras de materia fecal en el período comprendido de junio a octubre de 2021. Los análisis se realizaron en el Instituto Tecnológico de Boca del Río/Laboratorio de Investigación de Recursos Acuáticos, por la técnica de flotación de Willis y sedimentación en copa. Se identificó y cuantificó la presencia de helmintos como bioindicadores de contaminación por materia fecal. Resultados. 28 % de las muestras fueron positivas a formas parasitarias con una recuperación de 112 formas parasitarias, de las cuales el 67.74 % corresponden a parásitos del género Strongyloides sp. y anquilostomídeos. El 32.35 % corresponde a parásitos no patógenos para el hombre. De las muestras de materia fecal recolectadas solo 42.1 % mostraron positividad a formas parasitadas mencionadas anteriormente. Conclusiones. La presencia de formas parasitarias patógenas en las muestras de arena es un indicio de contaminación fecal que pone en riesgo la calidad sanitaria y ambiental de las playas arenosas de la zona conurbada Veracruz-Boca del Río y del Parque Nacional Sistema Arrecifal Veracruzano.

ABSTRACT. Technical and secure information on the production of quality atemoya seedlings (Annona squamosa L. x Annona cherimola Mill.) are of paramount importance due to the growing interest in this crop. This study aimed to assess the formation of atemoya seedlings propagated by grafting methods and cultivars using araticum (Annona sylvatica) rootstocks. The experiment was carried out in a screened environment at the Western Paraná State University (Unioeste), Campus of Marechal Cândido Rondon, Paraná State, Brazil. Atemoya grafts (Thompson, African Pride, and Gefner) were collected from plants from the CATI seedling nursery, located in São Bento do Sapucaí, São Paulo State, Brazil. Grafting was performed using 1-year-old araticum rootstocks with 8-10 mm in diameter at a height of 15 cm from the ground and grafts measuring, on average, 8.5 cm in length, 10 mm in diameter, and three buds. The experimental design consisted of randomized blocks in a 3 × 3 factorial arrangement (three grafting methods: cleft graft, whip and tongue graft, and chip budding × three cultivars: African Pride, Thompson, and Gefner), with four replications and 10 seedlings per replication, totaling 40 seedlings per treatment. The percentage of graft success (%), percentage of sprouting (%), number of sprouts, number of leaves, and longest sprout length (cm) were assessed at 60 days after grafting. The cleft graft method is efficient for producing atemoya seedlings. The cultivar African Pride can be grafted onto rootstocks by the cleft graft and whip and tongue graft methods, as they promote a higher percentage of graft success. The Annona sylvatica rootstock has potential for the production of atemoya seedlings, but chip budding is not viable. ABSTRACT L Mill. Mill crop Unioeste, Unioeste , (Unioeste) Rondon Brazil Thompson (Thompson Gefner nursery Sapucaí 1yearold yearold 1 year old 810 8 8-1 measuring average 85 5 8. buds Gefner, replication 4 treatment %, % (%) sprouts leaves (cm 6 viable (Unioeste 81 8- (% (

ABSTRACT Given the above, the objective of this study was to evaluate the bromatological, mineral and bioactive compounds of blackberry grown in a subtropical region. Blackberries fruits (Rubus sp.), Grown in an orchard of Unioeste, Campus Marechal Cândido Rondon (Paraná, Brazil), were used. Immediately after harvest, the fruits were taken to the Food Technology Laboratory for chemical analysis and bioactive compounds. The fruit samples for the analysis of reducing, bromatological and mineral sugars were frozen and sent to private laboratories. Hybrids are more perishable than cultivars. There is variation in color among the cultivars and hybrids studied. Hybrids and cultivars of black mulberry have a high content of ascorbic acid and fibers, with emphasis on the cultivar Tupy (75.0 mg 100 mL-1 and 7.23, respectively). Higher pH is verified in the cultivars Tupy and Arapaho (3.22 and 3.24, respectively). The Arapaho cultivar has fewer acid fruits (0.25 g 100 g-1), SS/total acidity ratio (36.88) and reducing sugar content (8.28 g 100g-1). Blackberry fruits are a rich source of bioactive compounds, such as cv. Chickasaw obtaining a higher content of total phenolic compounds (1368.84 mg EAG100mL-1) and the Boysenberry hybrid a greater amount of anthocyanin (5.11mg Ci-3-Gly g -1). The Chickasaw cultivar has a higher lipid content (4.59). There is no difference in moisture content, dry biomass and fruit firmness.

ABSTRACT The objective of this work was to evaluate asepsis protocols, composition and concentration of culture media in the in vitro establishment of three physalis species. In experiment I, seeds of 3 species were used x 3 asepsis protocols. In experiment II, 3 culture media and explants 3 species were used. In experiment III, explants of 2 species x 4 concentrations of MS culture medium were used. In experiment IV, explants of 2 species x 4 sucrose concentrations were used. For experiments II, III and IV after 30 days were evaluated some phytotechnical parameters. The experimental design was completely randomized, in a 3 x 3 (experiments I and II) and 2 x 4 (experiments III and IV) factorial scheme. Protocols II and III were appropriate for P. peruviana germination, and I and III for P. minima, the three protocols were efficient for controlling fungi and bacteria. The MS is the most suitable for the in vitro establishment of three species. The culture medium at 100% concentration obtained better values in the in vitro development parameters evaluated for the species P. peruviana and P. minima. Sucrose concentrations close to 50 and 20 g L-1 favored the establishment of P. peruviana and P. minima.

ABSTRACT The objective of this study was to evaluate the potential of rooting of podocarpos cuttings in function of the number of leaves, concentrations of IBA and phytorregulators, application methods and cutting season. Two experiments were carried out under weaning conditions, in a factorial scheme containing 3 replicates of 15 cuttings, the first with 4 concentrations of IBA, 3 leaves at the cuttings and 2 seasons. These were removed in August/2016 and January/2017, prepared with 10 cm in length and immersed in solutions for 10 s. In the second experiment three types of phytoregulators and 2 application forms were tested (slow and quick immersion). Cuttings were prepared in the same manner as in the first experiment, maintaining 4 leaves. The bases of the cuttings were immersed for 10 s and 24 h in the solutions, then taken to root. After 100 days of experimentation, it was concluded that cuttings collected in the summer had a higher percentage of rooting, 4 leaves and IBA concentration should be maintained between 750 and 800 mg L-1. The best form of application of phytoregulator is by rapid immersion.

Resumo Como a região oeste do Paraná é considerada subtropical, com altas temperaturas no verão, os híbridos de Encyclia são comercializados pela beleza de suas flores e durabilidade. No entanto, não existem estudos envolvendo plantas em vasos, tipos de substratos e fertilizantes, além dos híbridos para o gênero Encyclia. Diante do exposto, o objetivo deste trabalho foi avaliar o desenvolvimento vegetativo e o florescimento de Encyclia com base na adubação e no substrato.O experimento foi conduzido em um produtor comercial, de janeiro a setembro de 2017. As mudas permaneceram em ambiente protegido com 50% de sombreamento. O delineamento experimental foi em blocos casualizados, em esquema fatorial 4 x 5 (substratos x intervalos de fertilização), com 4 repetições por tratamento e 4 vasos por repetição. O lote escolhido era oriundo de semeadura in vitro, com o híbrido Encyclia “Kropp” x E. atropurpurea. Após cinco meses da última fertilização, foram realizadas avaliações relacionadas ao desenvolvimento vegetativo e floração. Os substratos à base de esfagno apresentaram melhores resultados no florescimento. As adubações orgânicas com Bokashi aos 45 dias após o transplante (DAT) favorecem a floração e o desenvolvimento vegetativo do híbrido de Encyclia, ocorrendo em vasos contendo substratos à base de esfagno e com adubações orgânicas acima de 60 DAT.

Abstract Due to the fact that the western region of Paraná is considered subtropical, with high temperatures in the summer, the hybrids of Encyclia are commercialized by the beauty of its flowers and durability. However, there are no studies involving potted plants, substrate types and fertilizers, as well as hybrids, for the genus Encyclia. Thus, the objective of this work was to evaluate the vegetative development and flowering of Encyclia based on fertilization and substrate. The experiment was conducted in a commercial orchard, from January to September/2017. The seedlings remained in a protected environment, with 50% shading. The experimental design was randomized blocks, in a 4 x 5 factorial scheme (substrates x fertilization intervals), with 4 replicates per treatment, and 4 pots per replicate. The lot chosen was sowed in vitro, with the hybrid Encyclia “Kropp” x E. atropurpurea. After five months of the last fertilization, the evaluations related to vegetative development and flowering were carried out. Substrates based on sphagnum presented better results in flowering. Organic fertilizations with Bokashi at 45 days after transplant (DAT) favor flowering and vegetative development of the Encyclia hybrid, which occurs in pots containing sphagnum-based substrates and with organic fertilizers above 60 DAT.

RESUMO O processo germinativo da Annona sylvatica A.St.-Hil. torna-se difícil porque as sementes apresentam dormência física e fisiológica. Porém, o ácido giberélico pode atuar na superação desse processo fisiológico. Assim, objetivou-se avaliar o efeito do ácido giberélico na superação de dormência em sementes de araticum. Foram desenvolvidos dois trabalhos distintos e simultaneamente (germinação de sementes em laboratório e emergência de plântulas em sementeira). Em laboratório, o delineamento experimental utilizado foi inteiramente casualizado em esquema fatorial 4 x 4 [quatro concentrações de ácido giberélico (0, 400, 800 e 1200 mg.L-1 x quatro períodos de imersão na solução (0, 8, 16 e 24 h)], avaliando-se os aspectos de germinação e vigor. A germinação de sementes apresentou desempenho superior quando foram embebidas em 1200 mg.L-1 de GA3 em 24 h de embebição, alcançando 67% de germinação. Na emergência de plântulas em sementeira, o delineamento experimental utilizado foi em blocos casualizados em esquema fatorial 4 x 5 [quatro acessos x cinco períodos de imersão (0, 18, 20, 22 e 24 h). Após 110 dias, avaliou-se aspectos fisiológicos de emergência e vigor. A emergência variou entre 32 a 45% (acessos A1 e A3) num período de 15 a 24 horas (acessos A1 e A3 ou A4). Alta concentração e maior período de embebição em ácido giberélico promove maior germinação de sementes de araticum. Existe diferença entre os acessos da espécie, necessário avaliação das mesmas antes da coleta, e no presente estudo o acesso A3 é promissor para coletas. A.St.Hil. AStHil A.St. Hil. St Hil A.St.-Hil tornase torna se fisiológica Porém fisiológico Assim objetivouse objetivou araticum sementeira. sementeira . sementeira) 0, 0 (0 400 80 120 mg.L1 mgL1 mgL mg.L 1 mg L mg.L- 8 2 h, , h)] avaliandose avaliando vigor GA 67 18 20 h. h) 11 dias avaliouse avaliou 3 45 A4. A4 A4) espécie coleta coletas A.St.Hil ASt A.St ( 40 12 L1 6

ABSTRACT The germination process of the Annona sylvatica A.St.-Hil becomes difficult because the seeds have physical and physiological numbness. Although, the objective was to evaluate the effect the gibberellic acid can have in overcoming this physiological process. So that, aimed to evaluate the effect of the gibberellic acid in the overcoming dormancy of araticum seeds. Two different projects were developed simultaneously (plants germination in the laboratory and emergence of seedlings in seed). At the laboratory, the experimental design used was completely casualized in factorial scheme 4 x 4. Evaluating the physiological aspect of the germination and vigor. The germination of the seeds presented superior performance when they were immersed in 1200 mg.L-1 of GA3, in 24 h of imbibition, reaching 67% of germination. In the emergence of seedlings in seed, the experimental design used was to randomize blocks in factorial scheme 4 x 5 (four accesses x five periods of immersion). After 110 days, evaluating physiological aspects of emergence and vigor. The emergence varied between 32 to 45% (accesses A1 and A3) in a period of 15 to 24 h (accesses A1 and A3 or A4). High concentration and longer period of soaking in gibberellic acid promote a bigger germination of the araticum seeds. There is a difference between the accesses of the species, necessary to evaluate before being collected, and in the present study the accesses A3 is promising for harvest. A.St.Hil AStHil A.St. Hil A St numbness Although that plants seed. seed . seed) vigor 120 mg.L1 mgL1 mgL mg.L 1 mg L mg.L- GA3 GA 2 imbibition 67 four immersion. immersion immersion) 11 days 3 45 A4. A4 A4) species collected harvest ASt A.St 12 L1 6

RESUMO: A propagação assexuada de coníferas, como o Podocarpus macrophyllus, apresenta dificuldades. Entre os fatores que podem tornar mais eficientes a propagação assexuada tem-se o tipo da estaca utilizada, lesões na base da estaca e uso de fitoreguladores, como o ácido indolbutírico (AIB). Objetivou-se determinar o tipo de lesão a ser efetuado na base da estaca e o tipo de estaca bem como método de veiculação do AIB na base da estaca e a concentração do AIB visando obter melhor enraizamento para a espécie. Dois experimentos foram realizados, no período de março a julho de 2018, em blocos casualizados e esquema fatorial, sendo o primeiro 2 x 4 [aplicação AIB em solução e em talco x concentração de 0, 500, 1000 e 1500 mg L-1 de ácido indolbutírico (AIB)] e o segundo 2 x 3 [estaca basal e apical x lesões na base da estaca do tipo bisel, cunha e horizontal].Em ambos experimentos as estacas utilizadas foram dispostas em canteiro de alvenaria contendo areia de textura média. Após 120 dias de experimentação conclui-se que o uso de estacas basais com lesões na base da estaca em cunha e tratadas com AIB em solução na concentração de aproximadamente 550 mL L-1favorecem a porcentagem de enraizamento.

ABSTRACT: The asexual propagation of conifers, such as Podocarpus macrophyllus (Thunb.) Sweet, faces difficulties. Among the factors that can improve the efficiency of asexual propagation are the types of cuttings used and lesions performed at the base of the cuttings, and the use of plant regulators, such as indolebutyric acid (IBA). This study determined the types of lesions to be performed at the cutting base, the type of cuttings used, the method to convey IBA at the cutting base, and the concentration of IBA required to obtain a proper rooting condition for the species. Two experiments were performed from March to July, 2018, in randomized blocks and with a factorial scheme, as follows: 2 x 4 (IBA application in solution and talc x IBA at concentrations of 0, 500, 1,000, and 1,500 mg L-1 IBA); and 2 x 3 (basal and apical cuttings x bevel, wedge, and horizontal lesions at the cutting base). In both experiments, the cuttings used were arranged on a masonry bed containing medium-textured sand. After 120 days of experimentation, the study concluded that the rooting percentage was favored by the use of basal cuttings with wedge lesions at the cutting base and treated with IBA in solution at a concentration of approximately 550 mg L-1.

RESUMO: Objetivou-se com o presente trabalho avaliar duas fontes alcoólicas no preparo, caracterização química e aceitabilidade de licores artesanais de doviális. Frutos de doviálisforam colhidos de plantas de 4 anos e imediatamente transportados ao laboratório. Chamou-se de L1 o licor que fora preparado com vodca + frutos sem epicarpo e L2 o licor preparado com aguardente de cana-de-açúcar + frutos com epicarpo e fatiados. Na sequência realizou-se a etapa de infusão ou maceração alcoólica, misturando os frutos + líquido alcoólico. Após o preparo do extrato alcoólico, foi realizada a caracterização química dos produtos finais e, após 60 dias, a análise sensorial e aceitação do produto. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 2 x 2 (2 tipos de frutos x 2 tipos de álcool), contendo três amostras de licores por repetição e seis repetições por parcela experimental. Os licores se enquadraram nos parâmetros previstos pela legislação brasileira na avaliação química dos produtos. Quanto a análise sensorial, houve boa aceitabilidade dos provadores do L1 e intenção de compra de 71% dos provadores.

ABSTRACT: This study evaluated two alcoholic sources in the preparation, chemical characterization, and acceptability of homemade Kei apple liqueurs. Kei apple fruits were harvested from 4-year-old plants and immediately transported to the laboratory. The liqueur prepared with vodka + fruits without the epicarp was named L1;the liqueur prepared with sugarcanespirit+ fruits with the epicarp and sliced was named L2. The infusion or alcoholic maceration stage was then performed, mixing the fruits with the alcoholic liquid. After the preparation of the alcoholic extract, the chemical characterization of the final products was performedand after 60 days, the sensory analysis and acceptabilityof the product were conducted. The experimental design was entirely randomized, in a 2 × 2factorial scheme (two types of fruits ×.two types of alcohol), containing three samples of liqueurs per replicate and six replicates per experimental plot. The liqueurs suited the parameters fixed by the Brazilian legislation for the chemical evaluation of these products. As for sensory analysis, L1 had good acceptability from tasters, with apurchase intention by 71% of them.

RESUMO: Objetivou-se com o presente trabalho avaliar a composição, aspectos nutricionais e análise sensorial da geleia de dovyalis. Frutos de dovyalis foram colhidos de plantas de quatro anos, no pomar da Fazenda Experimental da Unioeste. Estes, foram coletados de manhã, em caixas de polietileno, quando encontravam-se maduros e com coloração arroxeada do epicarpo, sendo imediatamente após, transportados ao laboratório, para o preparo das geleias. Após a chegada dos frutos, estes foram selecionados por tamanho, coloração e peso e, em seguida, higienizados em água corrente e retirados os cálices dos frutos. As avaliações sensoriais das amostras de geleia foram realizadas de forma casualizada, com 30 provadores voluntários. Os provadores foram previamente orientados a avaliarem a aceitação das geleias e suas intenções de compra. Frutos in natura e geleia de dovyalis são fontes de vitamina C. Frutos de dovyalis podem ser usados no processamento, devido sua acidez. A geleia preparada com frutos de dovyalis sem epicarpo e endocarpo, e menor quantidade de açúcar, foi mais apreciada pelos provadores.

ABSTRACT: The objective of this study is to evaluate the composition, nutritional aspects and sensory analysis of the dovyalis jam. Dovyalis fruits were harvested from 4-year-old plants in the orchard of the Unioeste Experimental Farm. They were collected in the morning in polyethylene boxes, when they were ripe and with a purple coloration from the epicarp, being immediately transported to the Laboratory to prepare the jams. After fruits arrival, they were selected by size, color and weight and then sanitized in running water and the cups of the fruits were removed. The sensory evaluations of the jam samples were performed in a randomized fashion, with 30 volunteer tasters. The tasters were previously advised to evaluate the acceptance of the jams and their purchase intentions. Fruits in natura and dovyalis jam are sources of vitamin C. Dovyalis fruits can be used in the processing, due to its acidity. The jam prepared with dovyalis fruits without epicarp and endocarp, and less sugar, was more appreciated by the tasters.

ABSTRACT The purpose this study was to evaluate the vegetative development, yield and quality of strawberry cultivars of short and neutral days as a function of soil cover. Two experiments were carried out in a protected environment. The experimental design used in both experiments consisted of randomized blocks, with subdivided plots. Harvests were carried out every two days, when the berries presented 75% of the red coloration of the epidermis, with evaluation of yield, number of fruits/plant, and fresh biomass of the fruits. For fruit quality, the pH, SS, titratable acidity, SS/TA ratio and fruit staining were evaluated. The contents of chlorophyll a and b, chlorophyll a + b and the chlorophyll a/b ratio in the leaves were also measured. At the end of the productive cycle, destructive evaluations of leaf number, leaf area and dry biomass of shoot were carried out. Cultivation on white plastic soil coverage presented plants with lower vegetative development, higher yield, and fruit size. Among the cultivars of short days, Camino Real presented bigger and more attractive fruits regarding the appearance of color of epidermis. For neutral day cultivars, Aromas obtained higher yield.

ABSTRACT The purpose of this study was to evaluate the agronomic behavior of the Niagara Rosada variety cultivated under plastic cover, using defoliation intensities, and its influence on the Plasmopara viticola. The experiment was conducted in Francisco Beltrão, PR, Brazil, in a vineyard of Niagara Rosada variety in a trellised system during the 2016/2017 and 2017/2018 crop seasons. The experimental design consisted of randomized blocks, in a 5 x 2 factorial scheme being five percentages of defoliation (0%, 10%, 20%, 30% and 40%,) and use of coverage (with and without coverage), with three blocks of three useful plants per experimental plot. The temperature, air humidity and incidence of mildew on the bunches and leaves were recorded. At the end of each crop station, three bunches per plant of each plot were evaluated for the fresh biomass of the bunches, length and width of the bunches, soluble solids content, pH, number of bunches per plant, production, and productivity. It was concluded that the plastic cover associated with the environmental conditions, and phytosanitary management can favor the physical-chemical quality and reduce the severity of the mildew in the Niagara Rosada variety providing greater production and productivity. The use of defoliation does not favor the cultivation of this variety in southwestern Paraná.

ABSTRACT The purpose of this study was to evaluate the phenological behavior and agronomic potential of blackberry varieties and hybrids in a subtropical region. Blackberry seedlings of four varieties and two hybrids were purchased in April 2015 in the form of stem cuttings and transplanting in July 2015. Winter pruning was carried out on August 18, 2016 and 2017. The experimental design was composed of a randomized complete block design, containing four varieties and two hybrids, four blocks and five useful plants per experimental unit. The harvest was performed every two days to the determination of fresh biomass, longitudinal and transverse diameter, volume, number of fruits per plant, yield and estimated productivity. The average duration of the phenological cycles in the two harvests varies between 109 and 162 days and 114 and 148, respectively. Hybrids Boysenberry and Olallie presented a shorter cycle in both harvests. In the 2016/2017 crop, the harvest lasted from 39 to 88 days. In the 2017/2018 harvest, it lasted 23 to 57 days, starting in mid-November through early January. The Tupy and Chickasaw cultivars were the most productive in the first crop. In the second crop, the Tupy and Navaho cultivars were prominent.

RESUMO O Equador tem um grande número de espécies nativas com propriedades fungicidas, herbicidas e inseticidas, das quais várias foram estudadas, no entanto, muito poucas espécies de plantas têm sido aplicadas no desenvolvimento de produtos comerciais. O Ocotea quixos, é uma planta indígena da Amazônia equatoriana, com propriedades fungicidas. Neste trabalho, nos concentramos na elaboração de um concentrado emulsionável que seja fisicamente estável para uso na indústria agrícola. O estudo teve como objetivo determinar a formulação adequada para preparar um concentrado emulsionável com estabilidade termodinâmica. Para a formulação, foi utilizado óleo essencial de Ocotea quixos com de cinamaldeído como ingrediente ativo, com solvesso 100 como solvente, dois emulsionantes não iónicos (Span-20, Tween-20) e fenil sulfonato de cálcio como emulsionante aniônico para obter um produto estável. Os resultados mostraram que o concentrado emulsionável OC5C possui as melhores características de estabilidade com o balanço hidrofílico lipofílico (HLB) dentro da faixa de 14 a 16 em temperatura ambiente assim como alta e baixa temperatura com tamanho de gota entre 3 e 4 μm.

ABSTRACT Ecuador has a vast number of native species with fungicidal, herbicidal, and insecticidal properties, of which several have been studied; however, few plant species have been applied for the development of commercial products. Ocotea quixos is an indigenous plant of the Ecuadorian Amazon that has fungicidal properties. In this work, we focus on developing an emulsifiable concentrate that is physically stable for use in the agricultural industry. The study aimed to determine the appropriate formulation to prepare an emulsifiable concentrate with thermodynamic stability. For the formulation, we used Ocotea quixos essential oil with cinnamaldehyde as an active ingredient, with solvesso 100 as the solvent, two non-ionic emulsifiers (Span-20 and Tween-20), and calcium phenyl sulfonate as an anionic emulsifier to obtain a stable product. The results showed that the OC5C emulsifiable concentrate has the best stability characteristics with the hydrophilic-lipophilic balance (HLB) within the range of 14 to 16 at room temperature as well as at high and low temperature with a drop size between 3 and 4 µm.