Abstract Regional sea level rise varies from the global average and is influenced by climate variability. We studied sea level anomalies in southern Brazil from 1993 to 2022, finding increasing trend from 1993 to 2022. We used oceanic and atmospheric dynamics to understand the rapid sea level rise. Positive trends in the Southern Annular Mode and the South Atlantic Ocean subtropical gyre intensified wind stress curl and Ekman transport. If global warming continues and the Southern Annular Mode remains in a positive trend, sea level rise in southern Brazil is likely to persist and increase risks for the population in this low-lying coastal area. variability 199 2022 transport lowlying low lying area 19 202 1 20 2

Resumo Caracterizar os efeitos da ingestão de gordura saturada na saúde metabólica e suas mudanças continua sendo um desafio importante. Dietas lipídicas, provenientes de diferentes fontes, variam amplamente em seus efeitos fisiológicos na saúde; portanto, é importante considerar a fonte lipídica específica consumida. O objetivo do estudo foi avaliar o efeito da imposição de dietas isocalóricas com diferentes fontes lipídicas (óleo de peixe/gordura suína) em zebrafish e descrever como parâmetros metabólicos, morfológicos e comportamentais podem se manifestar nesses peixes. Quarenta peixes fêmeas adultas foram utilizados para o experimento. Divididos em grupo Controle (C), alimentado com ração com ácido graxo insaturados e grupo ácido graxo saturado (Sat). Eles receberam comida três vezes ao dia, durante o período de 11 semanas. Os resultados mostraram que os animais no grupo Sat tiveram aumento de peso corporal, com diferença em relação ao grupo C, a partir da terceira semana da dieta até o final do experimento. No final da última semana, o grupo Sat teve um peso corporal 32% maior (P=0,0182) do que o peso do grupo controle. O consumo de uma dieta rica em ácidos graxos saturados não gerou sinais relacionados ao estresse e ansiedade em zebrafish. Houve um aumento na glicemia em T60 e T120, com diferença estatisticamente significativa entre os dois momentos. Animais no grupo Sat mostraram um aumento (P=0,0086) na esteatose hepática em comparação com animais no grupo controle. Os resultados obtidos sobre a relação entre a dieta e as alterações metabólicas são fundamentais para garantir a compreensão e o tratamento adequado desses problemas. portanto consumida óleo peixegordura peixe suína metabólicos experimento C , (C) Sat. . (Sat) dia 1 semanas 32 P=0,0182 P00182 P 0 0182 (P=0,0182 controle T T6 T120 momentos P=0,0086 P00086 0086 (P=0,0086 problemas (C (Sat 3 P=0,018 P0018 018 (P=0,018 T12 P=0,008 P0008 008 (P=0,008 P=0,01 P001 01 (P=0,01 T1 P=0,00 P000 00 (P=0,00 P=0,0 P00 (P=0,0 P=0, P0 (P=0, P=0 (P=0 P= (P= (P

Abstract Characterizing the effects of saturated fat intake on metabolic health and its changes remains a major challenge. Lipid diets, from different sources, vary widely in their physiological effects on health; therefore, it is important to consider the specific lipid source consumed. The objective of the study was to evaluate the effect of the imposition of isocaloric diets with different lipid sources in zebrafish (fish oil/pork lard). Depicting how metabolic, morphological and behavioral parameters might express themselves in these fishes. Forty adult female fishes were used for the experiment. The animals were divided into a control group (C), fed with unsaturated fatty acid diet, and a saturated fatty acid group (Sat). They received food three times a day, during the 11-week period. The results showed that animals in the Sat group had increased body weight, with a difference relative to the C group, from the third week of diet until the end of the experiment. At the end of the last week, the Sat group had a body weight 32% higher (P=0.0182) than the body weight of the control group. The consumption of a diet rich in saturated fatty acids did not generate signs related to stress and anxiety in zebrafish. There was an increase in glycemia at T60 and T120, with a statistically significant difference between the two moments. Animals in the Sat group showed an increase (P=0.0086) in hepatic steatosis compared to animals in the control group. The results obtained on the relationship between diet and metabolic changes are fundamental to ensure the understanding and appropriate treatment of these problems. challenge therefore consumed fish oilpork oil pork lard. lard . lard) experiment C, , (C) Sat. (Sat) day 11week 11 period 32 P=0.0182 P00182 P 0 0182 (P=0.0182 T T6 T120 moments P=0.0086 P00086 0086 (P=0.0086 problems (C (Sat 1 3 P=0.018 P0018 018 (P=0.018 T12 P=0.008 P0008 008 (P=0.008 P=0.01 P001 01 (P=0.01 T1 P=0.00 P000 00 (P=0.00 P=0.0 P00 (P=0.0 P=0. P0 (P=0. P=0 (P=0 P= (P= (P

Resumo O zebrafish têm sido usado em pesquisas há mais de 80 anos. Nas últimas 3 décadas, descobertas sobre as propriedades fundamentais do desenvolvimento, regeneração, câncer e outras doenças estabeleceram o peixe-zebra como um importante organismo modelo em pesquisas biomédicas. Este estudo teve como objetivo avaliar as alterações hepáticas em zebrafish, avaliando quantitativamente as áreas ocupadas por hepatócitos, bem como, tecidos conjuntivos e adiposos. Foram usados quarenta e oito Danio rerio (38 machos e 10 fêmeas), adultos, de aproximadamente 13 meses de idade. Foram divididos em quatro grupos, com 12 animais cada. Os peixes foram distribuídos aleatoriamente para formar os grupos, que receberam uma dieta de manutenção e/ou hipercalórica, com ou sem a adição de exercício físico. Os animais foram submetidos a seis horas de exercício forçado (5 cm/s) durante treze semanas. Os animais que praticaram exercício físico apresentaram maior densidade volumétrica da área ocupada pelos hepatócitos (65,92%±1,81 - GMex e 50,75%±2,24 GHex) dentre os grupos. Os animais do grupo GH apresentaram maior densidade volumétrica da área ocupada pelo tecido conjuntivo (15,12%±0,72), seguidos do grupo GHex (13,53%±1,43). Com relação densidade volumétrica da área ocupada pelo tecido adiposo, o grupo GH apresentou maior densidade (27,21%±1,36), seguido pelo grupo GHex (21,66%±1,11) com diferenças estatisticamente significativas. O GMex apresentou densidade volumétrica da área ocupada pelo tecido adiposo de 3,5%±0,76, enquanto o GM apresentou 5,7%±0,5, com diferença estatística. Relativamente os animais do grupo GHex apresentaram 20,39% menos gordura que os animais do grupo GH. Já os animais do grupo GMex apresentaram 72,47% menos gordura do que aqueles do GM. Concluiu-se que as diferentes constituições dietéticas e ainda a imposição de exercício físico foram capazes de modificar a arquitetura estrutural do fígado do Danio rerio. Sendo então critérios aceitáveis para que hajam modulações, visando assim, o controle e possíveis interferências relacionadas diretamente com o metabolismo da espécie e daí controle de doenças. 8 anos décadas desenvolvimento regeneração peixezebra peixe zebra biomédicas adiposos 38 (3 1 fêmeas, fêmeas , fêmeas) adultos idade grupos cada eou hipercalórica 5 ( cm/s cms cm s semanas 65,92%±1,81 6592181 65 92 81 (65,92%±1,8 5075224 50 75 2 24 50,75%±2,2 15,12%±0,72, 1512072 15,12%±0,72 15 0 72 (15,12%±0,72) 13,53%±1,43. 1353143 13,53%±1,43 . 53 43 (13,53%±1,43) 27,21%±1,36, 2721136 27,21%±1,36 27 21 36 (27,21%±1,36) 21,66%±1,11 2166111 66 11 (21,66%±1,11 significativas 35076 76 3,5%±0,76 5705 7 5,7%±0,5 estatística 2039 20 39 20,39 7247 47 72,47 Concluiuse Concluiu se modulações assim 65,92%±1,8 659218 6 9 (65,92%±1, 507522 50,75%±2, 151207 15,12%±0,7 (15,12%±0,72 135314 13,53%±1,4 4 (13,53%±1,43 272113 27,21%±1,3 (27,21%±1,36 21,66%±1,1 216611 (21,66%±1,1 3507 3,5%±0,7 570 5,7%±0, 203 20,3 724 72,4 65,92%±1, 65921 (65,92%±1 50752 50,75%±2 15120 15,12%±0, (15,12%±0,7 13531 13,53%±1, (13,53%±1,4 27211 27,21%±1, (27,21%±1,3 21,66%±1, 21661 (21,66%±1, 350 3,5%±0, 57 5,7%±0 20, 72, 65,92%±1 6592 (65,92%± 5075 50,75%± 1512 15,12%±0 (15,12%±0, 1353 13,53%±1 (13,53%±1, 2721 27,21%±1 (27,21%±1, 21,66%±1 2166 (21,66%±1 35 3,5%±0 5,7%± 65,92%± 659 (65,92% 507 50,75% 151 15,12%± (15,12%±0 135 13,53%± (13,53%±1 272 27,21%± (27,21%±1 21,66%± 216 (21,66%± 3,5%± 5,7% 65,92% (65,92 50,75 15,12% (15,12%± 13,53% (13,53%± 27,21% (27,21%± 21,66% (21,66% 3,5% 5,7 65,92 (65,9 50,7 15,12 (15,12% 13,53 (13,53% 27,21 (27,21% 21,66 (21,66 3,5 5, 65,9 (65, 50, 15,1 (15,12 13,5 (13,53 27,2 (27,21 21,6 (21,6 3, 65, (65 15, (15,1 13, (13,5 27, (27,2 21, (21, (6 (15, (13, (27, (21 (15 (13 (27 (2 (1

Abstract The zebrafish has been used in research for over 80 years. In the last three decades, discoveries about the fundamental properties of development, regeneration, cancer, and other diseases have established the zebrafish as an important model organism in biomedical research. This study aimed to evaluate liver alterations in zebrafish by quantitatively assessing the areas occupied by hepatocytes, as well as connective and adipose tissues. Forty-eight adult Danio rerio (38 males and 10 females) of approximately 13 months of age were used. They were divided into four groups, with 12 animals each. The fish were randomly distributed to form the groups, which received a maintenance and/or hypercaloric diet, with or without the addition of physical exercise. The animals underwent six hours of forced exercise (5 cm/s) for thirteen weeks. The animals that practiced physical exercise had a higher volumetric density of the area occupied by hepatocytes (65.92%±1.81 - GMex and 50.75%±2.24 GHex) among the groups. The GH group had a higher volumetric density of the area occupied by connective tissue (15.12%±0.72), followed by the GHex group (13.53%±1.43). Regarding the volumetric density of the area occupied by adipose tissue, the GH group had a higher density (27.21%±1.36), followed by the GHex group (21.66%±1.11) with statistically significant differences. The GMex had a volumetric density of the area occupied by adipose tissue of 3.5%±0.76, while the GM had 5.7%±0.5, with statistical difference. In relation to the animals in the GHex group, they had 20.39% less fat than the animals in the GH group. The animals in the GMex group had 72.47% less fat than those in the GM group. It is concluded that the different dietary constitutions and the imposition of physical exercise were able to modify the structural architecture of the liver of Danio rerio. These are acceptable criteria for modulations, thus aiming at the control and possible interferences directly related to the metabolism of the species and therefore the control of diseases. 8 years decades development regeneration cancer tissues Fortyeight Forty eight 38 (3 1 females groups each andor diet 5 ( cm/s cms cm s weeks 65.92%±1.81 6592181 65 92 81 (65.92%±1.8 5075224 50 75 2 24 50.75%±2.2 15.12%±0.72, 1512072 15.12%±0.72 , 15 0 72 (15.12%±0.72) 13.53%±1.43. 1353143 13.53%±1.43 . 53 43 (13.53%±1.43) 27.21%±1.36, 2721136 27.21%±1.36 27 21 36 (27.21%±1.36) 21.66%±1.11 2166111 66 11 (21.66%±1.11 differences 35076 3 76 3.5%±0.76 5705 7 5.7%±0.5 difference 2039 20 39 20.39 7247 47 72.47 modulations 65.92%±1.8 659218 6 9 (65.92%±1. 507522 50.75%±2. 151207 15.12%±0.7 (15.12%±0.72 135314 13.53%±1.4 4 (13.53%±1.43 272113 27.21%±1.3 (27.21%±1.36 21.66%±1.1 216611 (21.66%±1.1 3507 3.5%±0.7 570 5.7%±0. 203 20.3 724 72.4 65.92%±1. 65921 (65.92%±1 50752 50.75%±2 15120 15.12%±0. (15.12%±0.7 13531 13.53%±1. (13.53%±1.4 27211 27.21%±1. (27.21%±1.3 21.66%±1. 21661 (21.66%±1. 350 3.5%±0. 57 5.7%±0 20. 72. 65.92%±1 6592 (65.92%± 5075 50.75%± 1512 15.12%±0 (15.12%±0. 1353 13.53%±1 (13.53%±1. 2721 27.21%±1 (27.21%±1. 21.66%±1 2166 (21.66%±1 35 3.5%±0 5.7%± 65.92%± 659 (65.92% 507 50.75% 151 15.12%± (15.12%±0 135 13.53%± (13.53%±1 272 27.21%± (27.21%±1 21.66%± 216 (21.66%± 3.5%± 5.7% 65.92% (65.92 50.75 15.12% (15.12%± 13.53% (13.53%± 27.21% (27.21%± 21.66% (21.66% 3.5% 5.7 65.92 (65.9 50.7 15.12 (15.12% 13.53 (13.53% 27.21 (27.21% 21.66 (21.66 3.5 5. 65.9 (65. 50. 15.1 (15.12 13.5 (13.53 27.2 (27.21 21.6 (21.6 3. 65. (65 15. (15.1 13. (13.5 27. (27.2 21. (21. (6 (15. (13. (27. (21 (15 (13 (27 (2 (1

Resumo O objetivo do artigo é trazer informações acerca da socialização de gênero em quatro gerações da elite paulistana do século XX e XXI. Trata-se da análise de dados recolhidos na pesquisa Pensamento e práticas de cultura da elite paulistana. A intenção é problematizar experiências socializadoras de homens e mulheres — hetero e homossexuais — indagando sobre as possíveis transformações das formas de hierarquia de poder nestes grupos a partir dos habitus de gênero. Os dados foram recolhidos em entrevistas e questionários, com um olhar qualitativo, aplicados em um grupo de 48 pessoas. A hipótese principal apoia-se na teoria dos capitais de Pierre Bourdieu, entre outras, e suas formas subjetivas de construção de disposições de habitus. Destaca-se, por um lado, que as gerações e o sexo das pessoas passaram por mudanças expressivas, indicando rupturas individuais e sociais com alguns padrões estabelecidos para seu sexo ou geração. Por outro lado, indica-se também a permanência de um sentido prático que continua garantindo em todas as gerações as posições de status em função de suas expertises, subjetividades e berço familiar.

Abstract The objective of the article is to bring information about the socialization of gender in four generations of the São Paulo elite in the 20th and 21st centuries. This is the analysis of data collected in the research Thought and cultural practices of the elite paulistana. The intention is to problematize the socializing experiences of men and women - straight and homosexual - inquiring about the possible transformations of the forms of power hierarchy in these groups from the gender habitus. The data were collected in interviews and questionnaires, with a qualitative look, in a group of 48 people. The main hypothesis is based on Pierre Bourdieu’s theory of capital, among others, and its subjective forms of construction of habitus dispositions. It is noteworthy, on the one hand, that people’s generations and gender have undergone significant changes, indicating individual and social ruptures with some established standards for their gender or generation. On the other hand, it is also indicated the permanence of a practical sense that continues to guarantee status positions in all generations due to their expertise, subjectivities and family background.

Abstract Crustacean decapod larvae have developed several adaptations to maximize their survival, including swimming behavior. Swimming improves larvae dispersal and contributes to their response to environmental stresses, including variations in abiotic factors, presence of predators, and search for food. Data on larval behavior can also assist in studies on conservation and in species management in aquaculture. Unfortunately, swimming behavior remains unstudied in many species. The present work aims to analyze the swimming behavior in newly hatched larvae of six common decapod crustaceans in coastal Southeast Brazil (Macrobrachium acanthurus, Macrobrachium olfersii, Emerita brasiliensis, Ucides cordatus, Minuca rapax, and Callinectes danae). The analysis included tests with larval responses to four isolated stimuli: gravity (geotaxis), vertical salinity gradients, low-pressure zones, and presence of the planktivorous fish, Atherinella brasiliensis (only for C. danae and M. rapax). The first two and the last two stimuli were used as indicators of dispersal and defensive behaviors, respectively. All larvae preferred water depth with higher salinity levels. Additionally, negative geotaxis was observed in M. olfersii, M. acanthurus, U. cordatus, and C. danae, being stronger in the last. Both Macrobrachium larvae responded to low-pressure zones, promptly moving away. Callinectes danae and M. rapax larvae avoided contact with the predatory fish by creating avoidance zones around it, even in the absence of light. This is an active defensive behavior that has never been reported for the newly hatched larvae of these two species.

Resumo As raias violas Pseudobatos horkelii e Pseudobatos percellens, são listados como “Criticamente em Perigo” (CR) e “Em Perigo” (EN), respectivamente. Ambas as espécies ocorrem no Sul do Oceano Atlântico. Considerando a falta de dados sobre a estrutura genética dessas espécies, o presente estudo avaliou a variabilidade genética e a estrutura populacional de P. horkelii e P. percellens na região sudeste do Brasil e litoral norte da Argentina, com base em sequências de DNA mitocondrial, região de controle (D-loop). Amostras de 135 indivíduos de P. horkelii analisados em seis localidades, situadas no norte da Argentina e ao longo da costa dos estados brasileiros. A média da diversidade nucleotídica foi de 0.0053, o índice ΦST foi de 0.4277 e a análise demográfica de P. horkelii, indicou a existência de estabilidade das populações, com D = 0.9929, Fus = 2.0155, SSD = 0.0817, R = 0.2153. Em 101 exemplares de P. percellens, foram analisados em seis localidades brasileiras ao longo do litoral de Santa Catarina, Paraná e São Paulo. A diversidade nucleotídica média foi de 0.0014 e o valor ΦST de 0.2921, a análise demográfica indicou uma alta taxa de migração de P. percellens entre as localidades analisadas, com D = 0.5222, FS = 0.3528, SSD = 0.01785, R = 0.3890.

Abstract The guitarfishes Pseudobatos horkelii and Pseudobatos percellens meet the criteria for threatened status as Critically Endangered (CR) and Endangered (EN), respectively. Both species occur in the Southern Atlantic Ocean. Considering the lack of data on the genetic structure of these species, the present study evaluated the genetic variability and population structure of the P. horkelii and P. percellens in the southern region of Brazil and the northern coast of Argentina, based on sequences of mitochondrial DNA, Control Region (D-loop). Samples of P. horkelii (n = 135) were analyzed in six localities situated in Northern Argentina, along the Brazilian states’ coast. The mean of nucleotide diversity was 0.0053, the ΦST was 0.4277 and demographic analysis of P. horkelii suggests the existence of stability of the populations, with D = 0.9929, FS = 2.0155, SSD = 0.0817, R = 0.2153. In P. percellens (n = 101) were analyzed from six Brazilian localities along the coast of Santa Catarina, Paraná, and São Paulo. The mean nucleotide diversity was 0.0014 and ΦST value of 0.2921, the demographic analysis indicates a high migration rate of P. percellens among the localities evaluated, with D = 0.5222, FS = 0.3528, SSD = 0.01785, R = 0.3890.

RESUMO Rhinoptera bonasus é uma espécie bento-pelágica e altamente migratória, que ocorre do sul dos Estados Unidos ao norte da Argentina. Devido aos efeitos da sobrepesca, R. bonasus está atualmente em risco, classificada pela Lista Vermelha da IUCN como vulnerável. Considerando a falta de dados moleculares disponíveis para R. bonasus, este estudo teve como objetivo descrever a variabilidade genética e estrutura populacional de espécimes amostrados em três ecorregiões do litoral brasileiro (Ecorregião Amazônica, Pará; Ecorregião Nordeste, Pernambuco e Ecorregião Sudeste, Rio de Janeiro, São Paulo e Santa Catarina), por meio de cinco marcadores microssatélites polimórficos. Assim, testaremos as hipóteses de panmixia e filopatria natal. Um total de 69 espécimes analisados revelou diferenciação genética individual e significativa entre os locais amostrados. As análises de Φ ST (0,12), PCA, DAPC e Bayesiana revelaram pelo menos dois agrupamentos genéticos distintos de R. bonasus. Os testes de IBD foram significativos, indicando uma correlação entre a distância genética e geográfica entre as populações, o que pode ser explicado pelo comportamento filopátrico reprodutivo. O comportamento filopátrico associado à mobilidade de R. bonasus pode influenciar os valores de diferenciação observados para todos os loci nas amostras investigadas.

ABSTRACT Rhinoptera bonasus is a bento-pelagic and highly migratory species occurring from southern United States to northern Argentina. Due to overfishing effects, R. bonasus is currently at risk, classified by the IUCN Red List as vulnerable. Considering the lack of molecular data available for R. bonasus, this study aimed to describe the genetic variability and population structure of specimens sampled from three Brazilian coast ecoregions (Amazon ecoregion, Pará; Northeastern ecoregion, Pernambuco and Southeastern ecoregion, Rio de Janeiro, São Paulo and Santa Catarina), through five polymorphic microsatellite markers. Here testing the panmixia hypothesis for Brazilian ecoregions and test natal philopathy. A total of 69 analyzed specimens revealed individual and significant genetic differentiation between the sampled locations. Φ ST (0.12), PCA, DAPC and Bayesian analyses of the genetic population structure revealed at least two distinct genetic R. bonasus groupings. IBD tests were significant, indicating a correlation between genetic and geographical distance among populations, which can be explained by reproductive philopatric behavior. Philopatric behavior associated with R. bonasus mobility may influence the differentiation values observed for all loci in the investigated samples.

The use of bioprocess to convert low valued biomass or agroindustrial byproducts into high-value chemicals is an emerging area. However, laboratories usually use outdated analytical techniques to identify bioproducts, losing valuable information, such as the identification of unexpected compounds. In this work, we presented a successful systematic and modern analytical approach based on direct infusion mass spectrometry (DIMS) for an automated high-throughput screening of untargeted compounds from glycerin bioconversion process. We describe advantages of DIMS and its combined application with chemometrics towards an untargeted metabolomics approach to analyze several samples in a short time (11 samples per h). Three batches with 34 samples from the bioconversion of glycerin using several filamentous fungi strains were analyzed. Batch 347 was selected as promising, since high value chemicals, such as amide, phenolic and acid compounds, were identified. The platform presented was fast, robust, and versatile, then it could be applied to different bioprocesses.

Resumo: Um total de 1.471 espécimes de 16 espécies de linguados (Pleuronectiformes) foram capturados durante 48 campanhas de amostragem entre julho de 2005 e junho de 2007 em dez estações na Baía de Guanabara, Rio de Janeiro, Brasil. Paralichthyidae foi a família dominante, com Etropus crossotus como a espécie dominante. As estações externas, especialmente aquelas no lado ocidental do baixo estuário, foram distinguidas como resultado de sua maior abundância de linguados e número de espécies. A distribuição espacial de E. crossotus e sua estrutura populacional indicam que esta espécie é uma residente estuarina, apesar da aparente redução em sua área de ocupação dentro do complexo estuarino. Dentre as outras espécies, nove foram classificadas como migrantes marinhos (Achirus declivis, Bothus ocellatus, Cyclopsetta chittendeni, Etropus longimanus, Paralichthys orbignyanus, P. patagonicus, Syacium micrurum, Symphurus diomedeanus e Trinectes paulistanus) e três como oportunistas estuarinos (Bothus robinsi, Citharichthys macrops e Syacium papillosum); outras três não puderam ser classificados devido ao pequeno número de capturas ou falta de dados prévios (S. tessellatus, A. lineatus e C. spilopterus).

Abstract: A total of 1,471 specimens of 16 species of flatfishes (Pleuronectiformes) were caught during 48 sampling campaigns between July 2005 and June 2007 at ten stations in Guanabara Bay, Rio de Janeiro, Brazil. Paralichthyidae was the dominant family, with Etropus crossotus as the dominant species. The outer stations, especially those on the western side of the lower estuary, were distinguished as a result of their higher abundance of flatfishes and number of species. The spatial distribution of E. crossotus and its population structure indicate that this species is an estuarine resident despite the apparent reduction in its area of occupation within the estuarine complex. Among the other species, nine were classified as marine stragglers (Achirus declivis, Bothus ocellatus, Cyclopsetta chittendeni, Etropus longimanus, Paralichthys orbignyanus, P. patagonicus, Syacium micrurum, Symphurus diomedeanus and Trinectes paulistanus) and three as estuarine opportunists (Bothus robinsi, Citharichthys macrops and Syacium papillosum); another three could not be classified due to the small number of captures or lack of previous data (S. tessellatus, A. lineatus and C. spilopterus).

Abstract In the last decades, the modernization in structural engineering has increased the use of steel-concrete composite and hybrid systems for slabs, when the adherence in the interface of the materials is present or not, respectively. In addition to the traditional steel deck, a similar solution for precast trussed slabs has been used for small constructions, in which a cold formed U profile acts as steel formwork before the concrete curing, resisting to self-weight of the concrete and to the construction overload. After this period, the steel profile gets incorporated to the concrete element, allowing the composite behavior of the structure, system that usually goes without additional reinforcements. This slab includes, besides concrete, a light filling material between ribs, cold formed profiles and the trussed reinforcement. Once this technology has just arrived to the construction market, there is a gap of knowledge related to its design procedure. In this context, this study aims to present a methodology to analyze the limit-states that govern the design of these slabs. A computational tool was developed to evaluate the resistance through data entry related to geometry, service loads and materials, which grants to conduct a parametric study with pre-defined geometries to obtain, as result, spans and loads. Conclusions about the maximum span without shoring and general data are also discussed.

ABSTRACT Bloodstream and venous catheter-related corynebacterial infections in paediatric patients with haematological cancer were investigated from January 2003 to December 2014 at the Brazilian National Cancer Institute in Rio de Janeiro, Brazil. We observed that during cancer treatment, invasive corynebacterial infections occurred independent of certain factors, such as age and gender, underlying diseases and neutropenia. These infections were ssscaused by Corynebacterium amycolatum and other non-diphtherial corynebacteria. All cases presented a variable profile of susceptibility to antimicrobial agents, except to vancomycin. Targeted antibiotic therapy may contribute to catheters maintenance and support quality of treatment. Non-diphtherial corynebacteria must be recognized as agents associated with venous access infections. Our data highlight the need for the accurate identification of corynebacteria species, as well as antimicrobial susceptibility testing.

RESUMO: Theileria equi é um hemoprotozoário, agente da piroplasmose equina, doença de impacto sanitário e econômico internacional. Em éguas gestantes além da doença clínica, podem ocorrer abortos e danos ao neonato, caracterizando grande susceptibilidade à doença no período neonatal. O objetivo deste estudo foi avaliar a dinâmica da resposta imune humoral à EMA-2 recombinante de T. equi em éguas gestantes e potros, bem como a transferência de anticorpos vacinais no colostro. Foram utilizados 36 equinos, sendo 18 éguas gestantes e 18 potros. As éguas foram divididas em grupo controle e vacinado, que receberam rEMA-2 a partir dos 300 dias de gestação em três doses com intervalos de 21 dias. Para produção da vacina, o gene de expressão de EMA-2 foi clonado e a proteína expressa em Pichia pastoris. Os potros provenientes de éguas dos grupos vacinado e controle foram avaliados até o 6º mês de vida. Avaliou-se também a produção de anticorpos em potros submetidos ao esquema vacinal com rEMA-2 a partir do 2º mês de vida, que receberam três doses da vacina em intervalos de 21 dias. O método escolhido para a avaliação das amostras de soro e colostro foi ELISA indireto, com sensibilização pela proteína rEMA-2. Nas éguas gestantes vacinadas com rEMA-2 ocorreu o incremento de 2,3 vezes o valor basal ao final do esquema vacinal. O colostro de éguas vacinadas apresentou título médio de anticorpos de 1,0432±0,33, e potros provenientes de éguas vacinadas apresentaram média maior que os provenientes de éguas controle após a primeira mamada (12 horas). Os potros que passaram por esquema vacinal a partir do 2º mês de vida obtiveram incremento de 8,3 vezes o valor basal de anticorpos. O esquema vacinal com rEMA-2 foi capaz de estimular a imunidade humoral em éguas gestantes. Éguas gestantes vacinadas concentraram imunoglobulinas vacinais no colostro, e os potros provenientes destas obtiveram incremento nos níveis séricos de anticorpos vacinais após a primeira mamada.

ABSTRACT: Theileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking.

Resumo Crassostrea rhizophorae e C. gasar são cultivadas na região Nordeste. Parasitas Perkinsus infectam bivalves e seus efeitos em ostras de regiões tropicais são pouco compreendidos. Este estudo avaliou o impacto da infecção por Perkinsus em parâmetros de produção de ostras nativas. Ostras foram coletadas bimestralmente durante 7 meses, de julho de 2010 a fevereiro de 2011, para avaliar crescimento, mortalidade e padrão de coloração da concha (branca e cinza-escura) (n = 500); além da presença e intensidade de Perkinsus (n = 152). Perkinsus e Crassostrea foram identificados por abordagem molecular. Os resultados mostraram que as ostras cinza-escuras (90%, n = 20) e brancas (67%, n = 18) eram C. gasar e C. rhizophorae, respectivamente. As ostras mostraram uma boa taxa de crescimento e mortalidade acumulada moderada (44%). C. gasar cresceu melhor com menor mortalidade e menor incidência de Perkinsus que C. rhizophorae. A prevalência média de Perkinsus foi moderada (48%), mas a intensidade de infecção foi leve (2,2). A perkinsiose afetou ostras pequenas (19,4 mm). Em conclusão, ostras nativas, especialmente C. gasar, têm grande potencial de produção; sem mortalidade associada à perkinsiose; e, a cor da concha pode ser usada para melhorar a seleção de sementes com melhor desempenho.

Abstract Crassostrea rhizophorae and C. gasar oysters are cultivated in the northeast region. Perkinsus parasites infect bivalves, and their effects on oysters from tropical regions are poorly understood. This study evaluated the impact of Perkinsus infection on the productive traits of native oysters. Oysters were sampled bimonthly during 7 months, from July 2010 to February 2011, to evaluate growth rate, mortality and shell color patterns (white and dark-gray) (n = 500), and to determine the prevalence and intensity of Perkinsus (n = 152). Perkinsus and Crassostrea species were determined using molecular tools. Results showed that most dark-gray (90%, n = 20) and white (67%, n = 18) oysters were C. gasar and C. rhizophorae, respectively. Oysters showed a high growth rate and moderate cumulative mortality (44%). C. gasar oysters grew better and showed lower mortality and lower incidence of Perkinsus compared to C. rhizophorae. The mean prevalence of Perkinsus was moderate (48%), but the infection intensity was light (2.2). Perkinsosis affected very small oysters (19.4 mm). In conclusion, native oysters, especially C. gasar, have a great potential for culture, mortality is not associated with perkinsosis, and the shell color of oysters can be used to improve selection for spats with better performance.

Bahia Salado, located in northern Chile (27°41' S, 70°59'W), is the southernmost foraging ground for the endangered green turtle (Chelonia mydas) in the Eastern Pacific Ocean (EPO). To date, almost no information exists on its current status, nor on its connectivity with nesting rookeries in the EPO. This study aims to inform on the genetic characterization, health and ecology of Bahia Salado's green turtle aggregation in order to provide baseline information for local conservation strategies. We describe population structure and residency times using mark-recapture method. We also examine health parameters (body condition index, blood profile and blood copper-Cu and lead-Pb concentrations) and regional connectivity through genetic analyses. Our results indicate that this aggregation is composed exclusively of juveniles, with residency times varying between five to sixteen months. Turtles exhibited a very good body condition; however they showed the highest blood concentrations of Cu and Pb described for C. mydas and for almost all sea turtle species. Some biochemistry parameters (albumin, calcium, phosphorus, AST, triglycerides and creatinine) are also the highest ever reported for this species in the region. Analysis of the 770 bp (base pairs) control region of the mitochondrial DNA revealed four haplotypes, suggesting a strong genetic connectivity to the Galapagos rookery. Our study indicates that Bahia Salado's aggregation represents a developmental foraging ground, where juvenile green turtles thrive. Although Bahia Salado's ecosystem seems to be a very suitable habitat for the species, the high levels of Cu and Pb, together with elevated AST, demand further research on the negative impacts of heavy metals on this aggregation. Our results highlight the importance to protect this bay from anthropological activities, evaluate pollution sources and other local threats to this particular coastal ecosystem. We recommend year-round monitoring of the green turtle aggregation and other components of this ecosystem, incorporating participation of local seaweed collectors and the fishing community.