RESUMO Em regiões semiáridas, o uso de águas salobras na irrigação pode reduzir o rendimento das culturas. No entanto, a utilização da adubação mineral vem sendo testada para mitigar o estresse salino. Neste sentido, objetivou-se avaliar o efeito do estresse salino em diversos estágios fenológicos na produtividade do amendoim, sob adubação potássica. O experimento foi realizado no período de agosto a novembro de 2021, na área experimental da Universidade da Integração da Lusofonia Afro-Brasileira (UNILAB), Redenção, Ceará. O delineamento experimental foi o inteiramente casualizado (DIC), em um esquema fatorial 6 × 3, com 6 repetições. Foram utilizadas seis estratégias de irrigação com água salobra a partir das seguintes fases: vegetativa (S1); florescimento (S2); aparecimento dos ginóforos (S3); formação de vagens (S4); frutificação (S5) e sem estresse salino (S6) e três doses de potássio; 0, 50 e 100% da dose recomendada. O uso de água salobra na fase vegetativa evidenciou menor comprimento da vagem, massa das vagens, número de vagem, número de vagens comerciais e produtividade. A dose correspondente à 100% recomendada de potássio mitigou o estresse salino nas fases de formação de vagens e floração, proporcionando maior número de vagem comercial, não comercial e total e massa de vagem. O uso de água de menor salinidade durante todo o ciclo proporcionou maior massa de vagem, número de vagens comerciais e produtividade. semiáridas culturas entanto sentido objetivouse objetivou se amendoim potássica 2021 AfroBrasileira Afro Brasileira UNILAB, UNILAB , (UNILAB) Redenção Ceará DIC, DIC (DIC) 3 repetições S1 S (S1) S2 (S2) S3 (S3) S4 (S4) S5 (S5 S6 (S6 0 5 100 floração 202 (UNILAB (DIC (S1 (S2 (S3 (S4 (S 10 20 1 2

ABSTRACT In semi-arid regions, the use of brackish water for irrigation can reduce crop yields. However, the use of mineral fertilizer has been tested to mitigate salt stress. In this context, the objective was to evaluate the effect of salt stress at different phenological stages on the yield of peanut under potassium fertilization. The experiment was carried out from August to November 2021, in the experimental area of the Universidade da Integração da Lusofonia Afro-Brasileira (UNILAB), Redenção, Ceará, Brazil. The experimental design was completely randomized (CRD), in a 6 × 3 factorial scheme, with 6 replicates. Six strategies of irrigation with brackish water were applied from the following stages: vegetative (S1); flowering (S2); gynophore appearance (S3); pod formation (S4); fruiting (S5) and without salt stress (S6), and three doses of potassium: 0, 50 and 100% of the recommended dose. The use of brackish water in the vegetative stage led to lower pod length, pod mass, number of pods, number of marketable pods and yield. The dose corresponding to 100% of the recommended potassium dose mitigated salt stress in the pod formation and flowering stages, promoting a greater number of marketable pods, number of non-marketable pods, total number of pods and pod mass. The use of water with lower salinity throughout the cycle promoted greater pod mass, number of marketable pods and yield. semiarid semi arid regions yields However context fertilization 2021 AfroBrasileira Afro Brasileira UNILAB, UNILAB , (UNILAB) Redenção Ceará Brazil CRD, CRD (CRD) scheme replicates S1 S (S1) S2 (S2) S3 (S3) S4 (S4) S5 (S5 S6, S6 (S6) 0 5 100 length mass nonmarketable non 202 (UNILAB (CRD (S1 (S2 (S3 (S4 (S (S6 10 20 1 2

Resumo O excesso de sais na água de irrigação e o estresse hídrico, afetam negativamente o rendimento produtivo das culturas agrícolas. Neste sentido, objetivou-se avaliar o efeito do estresse salino associado ao hídrico no desempenho agronômico da cultura da beterraba. O experimento foi conduzido em casa de vegetação, em área pertencente a Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Redenção, Ceará. O delineamento experimental utilizado foi inteiramente casualizado, em parcelas subdivididas. As parcelas foram formadas pelas condutividades elétricas da água de irrigação (0,8; 1,5; 3,0; 4,5 e 6,0 dS m-1), enquanto os regimes hídricos de 50 e 100% da evapotranspiração da cultura (Etc), foram as subparcelas, com 6 repetições. O estresse salino afetou negativamente o crescimento, a biomassa, o comprimento da raiz tuberosa e a produtividade e aumentou os sólidos solúveis da cultura da beterraba. O excesso de sais da água de irrigação provoca reduções nos índices fisiológicas da cultura da beterraba, porém com menor severidade no regime hídrico de 100% da ETc. agrícolas sentido objetivouse objetivou se beterraba vegetação AfroBrasileira, AfroBrasileira Afro Brasileira, Brasileira Afro-Brasileira Redenção Ceará casualizado subdivididas 0,8 08 0 8 (0,8 1,5 15 1 5 3,0 30 3 45 4 4, 60 6, m1, m1 m , m-1) 100 Etc, Etc (Etc) subparcelas repetições crescimento biomassa ETc 0, (0, 1, 3, m-1 10 (Etc (0 m- (

Abstract Excessive salts in irrigation water and water stress have a negative impact on the productive yield of agricultural crops. In this regard, the objective was to evaluate the effect of combined saline and water stress on the agronomic performance of the beet crop. The experiment was conducted in a greenhouse located at the Universidade da Integração Internacional da Lusofonia Afro-Brasileira, in Redenção, Ceará. The experimental design used was completely randomized with split-plots arrangement. The main plots were formed by the electrical conductivities of the irrigation water (0.8, 1.5, 3.0, 4.5, and 6.0 dS m-1), while the irrigation depths of 50 and 100% of the crop evapotranspiration (ETc) were the subplots, with 6 replications. Saline stress negatively affected growth, biomass, tuber root length, and productivity, while increasing the soluble solids of the beet crop. Excessive salts in the irrigation water caused reductions in physiological indices of the beet crop, although with less severity under the 100% ETc. crops regard AfroBrasileira, AfroBrasileira Afro Brasileira, Brasileira Afro-Brasileira Redenção Ceará splitplots split arrangement 0.8, 08 0 8 (0.8 15 1 5 1.5 30 3 3.0 45 4 4.5 60 6. m1, m1 m , m-1) 100 ETc (ETc subplots replications growth biomass length productivity 0.8 (0. 1. 3. 4. m-1 10 0. (0 m- (

Abstract Arapaima gigas, an emblematic species of the Amazon region and a longstanding primary fishing resource, currently holds a “Data Deficient” status on the International Union for Conservation of Nature Red List, and is listed as an endangered species in Brazil. The Tocantins River is the most extensively modified large tributary of the Amazon Basin, and thus can affect the dynamics of ichthyofaunal populations. Over a period of 1 year, representatives of the fishing communities and fishermen from 25 fishing communities from four municipalities in the lower Tocantins River region were interviewed, and the obtained information was evaluated based on the literature to survey the population abundance status of A. gigas in the region and its impact on local communities. Among the fishermen interviewed, only one reported still encountering and fishing A. gigas on Jaracuera Island. The disappearance of A. gigas in the region are viewed as having economically disastrous consequences for the residents. Additionally, other endemic fish species are no longer observed in this locality either. If fishery management officials do not work together with local communities, A. gigas could disappear from the northern region of Brazil, where information on the dynamics of A. gigas fishing is lacking. resource Data Deficient List Brazil Basin populations year 2 interviewed A Island residents Additionally either lacking

Abstract Objective: To assess factors associated with emotional changes and Hyperactivity/Inattention (HI) motivated by COVID-19 quarantine in adolescents with immunocompromising diseases. Methods: A cross-sectional study included 343 adolescents with immunocompromising diseases and 108 healthy adolescents. Online questionnaires were answered including socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and validated surveys: Strengths and Difficulties Questionnaire (SDQ), Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0). Results: The frequencies of abnormal emotional SDQ scores from adolescents with chronic diseases were similar to those of healthy subjects (110/343 [32%] vs. 38/108 [35%], p = 0.548), as well as abnormal hyperactivity/inattention SDQ scores (79/343 [23%] vs. 29/108 [27%], p = 0.417). Logistic regression analysis of independent variables associated with abnormal emotional scores from adolescents with chronic diseases showed: female sex (Odds Ratio [OR = 3.76]; 95% Confidence Interval (95% CI) 2.00–7.05; p < 0.001), poor sleep quality (OR = 2.05; 95% CI 1.08–3.88; p = 0.028) and intrafamilial violence during pandemic (OR = 2.17; 95% CI 1.12–4.19; p = 0.021) as independently associated with abnormal emotional scores, whereas total PedsQL score was inversely associated with abnormal emotional scores (OR = 0.95; 95% CI 0.93–0.96; p < 0.0001). Logistic regression analysis associated with abnormal HI scores from patients evidenced that total PedsQL score (OR = 0.97; 95% CI 0.95–0.99; p = 0.010], changes in medical appointments during the pandemic (OR = 0.39; 95% CI 0.19–0.79; p = 0.021), and reliable COVID-19 information (OR = 0.35; 95% CI 0.16–0.77; p = 0.026) remained inversely associated with abnormal HI scores. Conclusion: The present study showed emotional and HI disturbances in adolescents with chronic immunosuppressive diseases during the COVID-19 pandemic. It reinforces the need to promptly implement a longitudinal program to protect the mental health of adolescents with and without chronic illnesses during future pandemics. Objective HyperactivityInattention Hyperactivity Inattention (HI COVID19 COVID 19 COVID-1 Methods crosssectional cross sectional 34 10 sociodemographic socio demographic selfrated self rated surveys SDQ, , (SDQ) PSQI, PSQI (PSQI) 40 4 0 4. PedsQL4.0. PedsQL40 PedsQL4.0 . PedsQL4 (PedsQL4.0) Results 110/343 110343 110 (110/34 32% 32 [32% vs 38108 38 38/10 35%, 35 35% [35%] 0.548, 0548 0.548 548 0.548) hyperactivityinattention hyperactivity inattention 79/343 79343 79 (79/34 23% 23 [23% 29108 29 29/10 27%, 27 27% [27%] 0.417. 0417 0.417 417 0.417) Odds OR 3.76 376 3 76 3.76] 95 (95 2.00–7.05 200705 2 00 7 05 0.001, 0001 0.001 001 0.001) 2.05 205 1.08–3.88 108388 1 08 88 0.028 0028 028 2.17 217 17 1.12–4.19 112419 12 0.021 0021 021 0.95 095 0.93–0.96 093096 93 96 0.0001. 00001 0.0001 0.0001) 0.97 097 97 0.95–0.99 095099 99 0.010, 0010 0.010 010 0.010] 0.39 039 39 0.19–0.79 019079 0.021, 0.35 035 0.16–0.77 016077 16 77 0.026 0026 026 Conclusion pandemics COVID1 COVID- (SDQ (PSQI PedsQL4. (PedsQL4.0 110/34 11034 11 (110/3 [32 3810 38/1 [35% 054 0.54 54 79/34 7934 (79/3 [23 2910 29/1 [27% 041 0.41 41 3.7 37 9 (9 2.00–7.0 20070 000 0.00 2.0 20 1.08–3.8 10838 8 0.02 002 02 2.1 21 1.12–4.1 11241 0.9 09 0.93–0.9 09309 0000 0.000 0.95–0.9 09509 0.01 01 0.3 03 0.19–0.7 01907 0.16–0.7 01607 (PedsQL4. 110/3 1103 (110/ [3 381 38/ [35 0.5 5 79/3 793 (79/ [2 291 29/ [27 04 0.4 3. ( 2.00–7. 2007 0.0 2. 1.08–3. 1083 1.12–4. 1124 0. 0.93–0. 0930 0.95–0. 0950 0.19–0. 0190 0.16–0. 0160 (PedsQL4 110/ (110 [ 79/ (79 2.00–7 200 1.08–3 1.12–4 112 0.93–0 093 0.95–0 0.19–0 019 0.16–0 016 (PedsQL (11 (7 2.00– 1.08– 1.12– 0.93– 0.95– 0.19– 0.16– (1 2.00 1.08 1.12 0.93 0.19 0.16 1.0 1.1 0.1 1.

Resumo As bactérias responsáveis por causar infecções são comuns em ambientes hospitalares, água, solo e produtos alimentícios. O risco de infecção é intensificado pela ausência de saneamento público, má qualidade de vida e escassez de alimentos. Esses fatores externos promovem a disseminação de patógenos por contaminação direta ou formação de biofilme. Neste trabalho, identificamos isolados bacterianos obtidos de unidades de terapia intensiva na região sul do Tocantins, Brasil. Comparamos técnicas de espectrometria de massa de tempo de voo com ionização por dessorção a laser assistida por matriz (MALDI-TOF MS) e análise molecular de ácido ribonucleico ribossômico 16S (rRNA); também realizamos caracterização fenotípica. Cinquenta e seis isolados caracterizados por testes morfotintoriais foram classificados como gram-positivos (80,3%; n = 45) e gram-negativos (19,6%; n = 11) e foram resistentes a várias classes de antibióticos; notavelmente, identificamos o gene de resistência blaOXA-23 no isolado de ILH10. A identificação microbiana usando MALDI-TOF MS resultou na identificação de Sphingomonas paucimobilis e Bacillus circulans. O sequenciamento do 16S rRNA revelou quatro isolados pertencentes aos gêneros Bacillus e Acinetobacter. A similaridade foi superior a 99% para Acinetobacter schindleri no BLAST, agrupado no clado superior a 90%. Várias cepas isoladas de ICU foram resistentes a várias classes de antibióticos. Essas técnicas permitiram a identificação de diversos microrganismos de importância em saúde pública, possibilitando melhorias no controle de infecções humanas e comprovando a qualidade dos insumos, alimentos e água. hospitalares água alimentícios público biofilme trabalho Tocantins Brasil MALDITOF MALDI TOF S (rRNA) fenotípica grampositivos gram positivos 80,3% 803 80 3 (80,3% 45 gramnegativos negativos 19,6% 196 19 6 (19,6% 11 antibióticos notavelmente blaOXA23 blaOXA 23 blaOXA-2 ILH10 ILH circulans 99 BLAST 90 90% pública insumos (rRNA 80,3 8 (80,3 4 19,6 1 (19,6 blaOXA2 2 blaOXA- ILH1 9 80, (80, 19, (19, (80 (19 (8 (1 (

Abstract Bacteria responsible for causing infections are common in hospital environments, water, soil, and food products. The infection risk is intensified by the absence of public sanitation, poor quality of life, and food scarcity. These external factors promote the dissemination of pathogens by direct contamination or biofilm formation. In this work, we identified bacterial isolates obtained from intensive care units in the southern region of Tocantins, Brazil. We compared matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) techniques and 16S ribosomal ribonucleic acid (rRNA) molecular analysis; we also performed phenotypic characterization. Fifty-six isolates characterized using morphotinctorial tests were classified as gram-positive (80.4%; n = 45) and gram-negative (19.6%; n = 11) and were resistant to several antibiotic classes; notably, we identified the blaOXA-23 resistance gene in the ILH10 isolate. Microbial identification using MALDI-TOF MS resulted in the identification of Sphingomonas paucimobilis and Bacillus circulans. 16S rRNA sequencing revealed four isolates belonging to the genera Bacillus and Acinetobacter. The similarity was superior to 99% for Acinetobacter schindleri in the Basic Local Alignment Search Tool (BLAST), grouped in the clade superior to 90%. Several strains isolated from intensive care units (ICU) were resistant to various antibiotic classes. These techniques allowed for the identification of several microorganisms of importance in public health, enabling improvements in human infection control and proving the quality of inputs, food, and water. environments water soil products sanitation life scarcity formation work Tocantins Brazil matrixassisted matrix assisted timeofflight time flight MALDITOF MALDI TOF S (rRNA analysis characterization Fiftysix Fifty six grampositive gram positive 80.4% 804 80 4 (80.4% 45 gramnegative negative 19.6% 196 19 6 (19.6% 11 classes notably blaOXA23 blaOXA 23 blaOXA-2 ILH ILH1 isolate circulans 99 BLAST, BLAST , (BLAST) 90 90% ICU (ICU health inputs 80.4 8 (80.4 19.6 1 (19.6 blaOXA2 2 blaOXA- 9 (BLAST 80. (80. 19. (19. (80 (19 (8 (1 (

RESUMO A região semiárida apresenta o problema inerente de acúmulo de sais no solo devido ao uso de irrigação com água salobra, e o déficit hídrico que compromete o crescimento e os índices fisiológicos das culturas. Objetivou-se com este trabalho avaliar o efeito do estresse salino e hídrico no crescimento e nas trocas gasosas da cultura da abobrinha cv. Caserta. O trabalho foi realizado em área pertencente à Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Redenção, Ceará, Brasil. O delineamento experimental utilizado foi inteiramente casualizado, em esquema fatorial 5 × 2 com cinco níveis de condutividade elétrica da água de irrigação - CEa (0,5, 1,0, 1,5, 2,0 e 2,5 dS m-1), e dois regimes hídricos (50 e 100% da evapotranspiração potencial da cultura - ETc) com cinco repetições. Aos 36 dias após a semeadura foram avaliadas as variaveis:taxa de assimilação de CO2, transpiração, condutância estomática, concentração interna de carbono, temperatura foliar, índice relativo de clorofila e a eficiência do uso da água. Aos 45 dias após a semeadura, foram avaliadas as seguintes variáveis: altura da planta, diâmetro do caule, número de folhas e área foliar. O uso de água salobra (1 a 2,5 dS m-1) reduz os parâmetros de crescimento da abobrinha cv. Caserta. O aumento da CEa ocasionou redução nas variáveis fisiológicas. A lâmina de 100% proporcionou maiores valores para as variáveis taxa de assimilação de CO2, transpiração, e eficiência instantânea no uso da água, e a lâmina de 50% para concentração interna de carbono, com o aumento da CEa.

ABSTRACT Semi-arid regions present the inherent problem of accumulation of salts in the soil due to the use of brackish water for irrigation, and water deficit compromises the growth and physiological indices of crops. This study evaluated the effect of salt and water stress on growth and gas exchange in the zucchini cv. Caserta crop. The study was conducted at the University of International Integration of Afro-Brazilian Lusophony, Redenção, Ceará State, Brazil. The experimental design was completely randomized, in a 5 × 2 factorial scheme with five levels of electrical conductivity of the irrigation water - ECw (0.5, 1.0, 1.5, 2.0, and 2.5 dS m-1) and two water regimes (50 and 100% of the potential crop evapotranspiration - ETc), with five replicates. At 36 days after sowing, the following traits were evaluated: CO2 assimilation rate, transpiration, stomatal conductance, internal carbon concentration, leaf temperature, relative chlorophyll index, and water use efficiency. At 45 days after sowing, the plant height, stem diameter, number of leaves, and leaf area were also evaluated. The use of brackish water (1 to 2.5 dS m-1) reduced the growth parameters of zucchini cv. Caserta. The increase in ECw caused a decline in the physiological traits. Under 100% ETc, higher values of CO2 assimilation rate, transpiration, and instantaneous water use efficiency were recorded, and there was 50% ETc for internal carbon concentration, even with the increase in ECw.

RESUMO A salinidade da água de irrigação pode causar sérios problemas na produção agrícola, enquanto as fontes de fertilizantes orgânicos potencialmente mitigam o estresse salino. Assim, este trabalho teve como objetivo avaliar a produtividade da cultura do milho sob irrigação com água salina e adubação orgânica. O estudo foi conduzido em campo de agosto a novembro de 2020 na fazenda experimental da Universidade da Integração Internacional da Lusofonia Afro-Brasileira (UNILAB), Redenção-CE. Utilizou-se o delineamento em blocos casualizados, em parcelas subdivididas, em que as parcelas consistiram em dois níveis de salinidade da água de irrigação (0,8 e 3,0 dS m-1). Nas subparcelas foram aplicadas quatro combinações de fontes de adubo orgânico, compostas por 10 plantas cada, com quatro repetições: C1, esterco bovino + biofertilizante de aves + biofertilizante de cabra; C2, esterco bovino + biofertilizante caprino; C3, esterco bovino + biofertilizante de aves; e C4, tratamento controle. A combinação de fontes de fertilizantes orgânicos não influenciou o acúmulo de assimilados e a produtividade das lavouras de milho sob a salinidade da água de irrigação de 3,0 dS m-1, exceto C3, que atenuou o efeito salino.

ABSTRACT Irrigation water salinity can cause serious problems in crop production, while organic fertilizer sources potentially mitigate saline stress. Thus, this study aimed to evaluate the productivity of maize crops under irrigation with saline water and organic fertilization. The study was conducted in the field from August to November 2020 at the experimental farm of the Universidade da Integração Internacional da Lusofonia Afro-Brasileira (UNILAB), Redenção-CE. A randomized block design was used, in subdivided plots, in which the plots consisted of two levels of irrigation water salinity (0.8 and 3.0 dS m-1). Four combinations of organic fertilizer sources were applied in the subplots, composed of 10 plants each, with four replications: C1, cattle manure + poultry biofertilizer + goat biofertilizer; C2, cattle manure + goat biofertilizer; C3, cattle manure + poultry biofertilizer; and C4, control treatment. The combination of organic fertilizer sources did not influence the accumulation of assimilates and productivity of maize crops under the irrigation water salinity of 3.0 dS m-1, except for C3, which attenuated the saline effect.

The antifungal potential of volatile organic compounds (VOCs) produced by actinobacterial strains Streptomyces sp. (ACTB-77) and Amycolatopsis sp. (ACTB-290) from the rhizosphere of Caatinga plants against Colletotrichum musae was investigated. VOCs produced by these microorganisms (axenic and co-culture) were investigated using headspace-solid phase micro-extraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). Although no exclusive VOC peaks were observed in the co-culture with ACTB-77, the same experiment involving ACTB-290 yielded five new peaks, including two identified alcohols, suggested as bioreductive products of the corresponding ketones by the fungus. Statistical analysis revealed that co-culture ACTB-77/C. musae has a closer similarity to the fungus than to the actinobacteria, while the co culture ACTB-290/C. musae showed closer similarity to the actinobacteria. These confirmed the more pronounced antifungal activity of the ACTB-290 strain, as observed in the fungus growth inhibition experiments. The antifungal activity of ACTB-290 was associated to its sulfur-containing metabolites, while linalool was suggested as responsible for the ACTB-77 activity.

RESUMO A cultura do amendoim apresenta grande interesse econômico, em decorrência de suas sementes, constituírem importante fonte de proteína vegetal e de óleo comestível. O objetivo do presente trabalho foi avaliar a fisiologia e crescimento da cultura do amendoim submetido a estresse salino e hídrico. O experimento foi conduzido em Auroras, Redenção, Ceará, Brasil. O delineamento experimental foi inteiramente casualizado em parcelas subdivididas, cujas parcelas referem-se aos níveis de condutividade elétrica da água de irrigação - CEa (1,0; 2,0; 3,0; 4,0 e 5,0 dS m-1) e as subparcelas aos regimes hídricos de 50 e 100% da evapotranspiração da cultura. O estresse salino afeta negativamente a altura de planta, número de folhas e massa seca da parte aérea, índices relativos de clorofila, transpiração e a temperatura foliar da cultura do amendoim. O regime hídrico de 100% da ETc proporcionou maiores desempenhos para as variáveis diâmetro do caule, número de folhas e massa seca da parte aérea da cultura do amendoim.

ABSTRACT Peanut crops are of great economic interest because their seeds are used as an important source of vegetable protein and edible oil. The objective of this study was to evaluate the physiological indices and growth of peanut crops subjected to saline and water stress. The experiment was conducted in Auroras, Redenção, Ceará, Brazil using a completely randomized split plot statistical design. The plots were composed of irrigation water treatments with varying levels of electrical conductivity - ECw (1.0, 2.0, 3.0, 4.0 and 5.0 dS m-1), while the subplots were formed by the water regimes of 50% and 100% crop evapotranspiration. Plant height, leaf number, shoot dry matter, relative chlorophyll level, transpiration, and leaf temperature were affected by salt stress. The water regime of 100% ETc provided better performance for the variables stem diameter, leaf number, and shoot dry mass of peanuts.

ABSTRACT The lowest dosage of empagliflozin (10 mg) showed similar benefits on glycated hemoglobin (HbA1c) level, body weight, blood pressure, and total and cardiovascular mortality in comparison with the highest available dose (25 mg) in the EMPAREG trial. These findings have not been clearly demonstrated for canagliflozin and dapagliflozin. The objective was to compare the effect of different doses of SGLT2 inhibitors commercially available in Brazil on HbA1c and body weight of patients with type 2 diabetes. MEDLINE, Cochrane and Embase databases were searched from inception until 11th October 2021 for randomized controlled trials of SGLT2 inhibitors in type 2 diabetes patients, lasting at least 12 weeks. HbA1c and body weight variations were described using standard mean difference. We performed direct and indirect meta-analysis, as well as a meta-regression with medication doses as covariates. Eighteen studies were included, comprising 16,095 patients. In the direct meta-analysis, SGLT2 inhibitors reduced HbA1c by 0.62% (95% CI −0.66 to −0.59) and body weight by 0.60 kg (95% CI −0.64 to −0.55). In the indirect meta-analysis, canagliflozin 300 mg ranked the highest regarding reductions in HbA1c and body weight. The remaining medications and dosages were clinically similar, despite some statistically significant differences among them. Canagliflozin 300 mg seems to be more potent in reducing HbA1c and body weight in patients with type 2 diabetes. The remaining SGLT2 inhibitors at different doses lead to similar effects for both outcomes. Whether these glycemic and weight effects are reflected in lower mortality and cardiovascular events is still uncertain and may be a topic for further studies.

Resumo Objetivo: O objetivo principal deste artigo é compreender o comportamento de jovens em início de carreira no contexto da Covid-19, considerando a intenção de continuar utilizando os serviços da rede social profissional LinkedIn. Originalidade/valor: Este artigo amplia o escopo dos estudos de aceitação na intenção de continuar utilizando (uso contínuo) o LinkedIn, analisando uma série de fatores positivos e negativos em relação aos processos. Design/metodologia/abordagem: Realizou-se uma pesquisa descritiva com abordagem quantitativa. Os autores coletaram dados de um survey (n = 386) e, com o objetivo de testar as hipóteses, aplicaram a técnica de modelagem de equações estruturais (MEE), com estimação de mínimos quadrados parciais, e um efeito moderador com o construto otimismo tecnológico para as variáveis preditivas da intenção de continuar utilizando. Resultados: Os resultados indicaram que todas as relações diretas e indiretas foram aceitas, exceto uma moderação (H7d), que foi rejeitada, visto que os usuários menos otimistas com a tecnologia podem se sentir positivamente sobrecarregados quanto ao nível de utilidade percebida do serviço. A variedade de serviços do LinkedIn pode levar o usuário a uma intenção de continuidade no uso da plataforma, uma vez que o objetivo dessa rede é facilitar a autoapresentação coletiva, mantendo as relações profissionais ativas. Os resultados também indicaram que os entrevistados da amostra são mais propensos a se envolverem ativa e semipassivamente no LinkedIn.

Abstract Purpose: The primary purpose of this article is to understand the behavior of young people at the beginning of their careers, in the context of Covid-19, considering the intention to continue using the services of the professional social network LinkedIn. Originality/value: This article expands the scope of studying a user group from acceptance in the continuance intention (continuous use) of LinkedIn, analyzing a series of positive and negative factors regarding the processes. Design/methodology/approach: We carried out a descriptive quantitative approach. The authors collected data from a survey (n = 386). To test the hypotheses, the researchers applied the technique of structural equation modeling (SEM), with estimation and partial least squares, and a moderating effect with the technological optimism construct for the predictive variables of the intention to continue using. Findings: The results indicated that all direct and indirect relationships were accepted, except a moderation (H7d) which was rejected, considering that users less optimistic about the technology may feel positively overwhelmed regarding the service’s perceived usefulness. LinkedIn’s variety of services can lead users to continue using the platform. The platform’s objective is to facilitate collective self-presentation, keeping profes sional relationships active. The results also indicated that the sample respondents are more likely to be actively and semi-passively involved on LinkedIn.

Resumo Bacillus thuringiensis é o entomopatógeno mais utilizado no controle do Aedes aegypti, vetor de diferentes agentes etiológicos que causam infecções graves em humanos. Diversos estudos têm como objetivo isolar cepas dessa bactéria de diferentes ambientes, com a perspectiva de selecionar isolados com atividade larvicida para mosquitos. Visando a ação inseticida de B. thuringiensis, o presente estudo teve como objetivo prospectar B. thuringiensis de solos de restinga e mangue do estado do Maranhão, Brasil, com potencial tóxico para uso no controle biológico de Ae. aegypti. Bioensaios foram realizados para determinar a atividade entomopatogênica do bacilo contra Ae. aegypti e as concentrações letais (CL50 e CL90) foram estimadas após os testes. As técnicas de Reação em Cadeia da Polimerase e SDS-PAGE foram realizadas para verificar o conteúdo de genes e proteínas dos isolados, respectivamente. Os solos dos ecossistemas de mangue e restinga apresentaram potencial para obtenção de B. thuringiensis. O isolado BtMA-750, obtido a partir da amostra de solo da restinga, foi interessantemente distinguido por sua alta toxicidade para Ae. aegypti. Este isolado, além de apresentar proteínas com massa molecular semelhante às toxinas Cry e Cyt, apresentou também diversos genes díptero-específicos cry e cyt, demonstrando que tem alto potencial para ser usado no controle biológico de Ae. aegypti.

Abstract Bacillus thuringiensis is the most commonly used entomopathogen in the control of Aedes aegypti, which is a vector for different etiological agents that cause serious infections in humans. Several studies aim to isolate strains of this bacterium from different environments, with the perspective of selecting isolates with larvicidal activity for mosquitoes. Aiming at the insecticidal action of B. thuringiensis, the present study aimed to prospect B. thuringiensis of restinga and mangrove soils from the state of Maranhão, Brazil, with toxic potential for use in the biological control of Ae. aegypti. Bioassays were performed to determine the entomopathogenic activity of the bacilli against Ae. aegypti and lethal concentrations (LC50 and CL90) were estimated after the tests. Polymerase Chain Reaction and SDS-PAGE techniques were performed to verify the gene and protein content of the isolates, respectively. The soil of the mangrove and restinga ecosystems showed potential for obtaining B. thuringiensis. This isolate, in addition to having proteins with molecular mass similar to the toxins Cry and Cyt, also presented several diptera-specific genes cry and cyt, demonstrating that it has high potential to be used in the biological control of Ae. aegypti.

Resumo Espécies de Trichogramma Westwood, 1833 (Hymenoptera: Trichogrammatidae) são freqüentemente usadas como agentes de controle biológico contra Lepidoptera, esses endoparasitóides de ovos apresentam taxonomia complexa, o que dificulta sua aplicação prática. Este estudo teve como objetivo comparar seqüências de regiões espaçadoras internas transcritas de DNA ribossômico (ITS2-rDNA) de acessos de Trichogramma com aquelas depositadas no GenBank, a fim de avaliar a confiabilidade do ITS2 barcode para discriminar espécies e avaliar a diversidade genética. As seqüências de ITS2-rDNA obtidas de dezessete espécimes de Trichogramma confirmaram identidades anteriores com base em características morfológicas. O alinhamento de múltiplas sequências revelou a existência de regiões altamente conservadas nas sequências ITS2, enquanto o dendrograma indicou que os espécimes formavam três grupos compreendendo T. manicobai e T. marandobai (grupo I), T. galloi (grupo II) e T. pretiosum (grupo III). O marcador ITS2 mostrou ser um poderoso DNA barcode para discriminar espécies de Trichogramma podendo ser usado como complemento da abordagem morfológica.

Abstract Species of Trichogramma Westwood, 1833 (Hymenoptera: Trichogrammtidae) are frequently used as biological control agents against Lepidoptera, but practical application of these egg endoparasitoids are complicated because of their complex taxonomy. This study aimed to compare sequences of internal transcribed spacer regions of ribosomal DNA (ITS2-rDNA) of Trichogramma accessions with those deposited in GenBank in order to access the reliability of the ITS2 as a barcode for discriminating species and evaluating the genetic diversity. ITS2-rDNA sequences obtained from seventeen specimens of Trichogramma confirmed previous identifications based on morphological characteristics. Multiple sequence alignment revealed the existence of highly conserved regions in ITS2 sequences while the neighbour-joining dendrogram indicated that the specimens formed three clusters comprising T. manicobai and T. marandobai (group I), T. galloi (group II) and T. pretiosum (group III). The ITS2 marker was shown to be a powerful DNA barcode for discriminating Trichogramma species and could be used to complement the morphological approach.

Resumo Os agentes entomopatógenos são alternativas viáveis e eficazes, devido à sua ação seletiva para insetos sendo inofensivos ao homem e ao meio ambiente. Dentre os entomopatógenos mais promissores, destacam-se as subespécies de Bacillus thuringiensis (Bt) amplamente utilizadas no controle biológico de insetos incluindo espécies de mosquitos vetores de agentes patogênicos ao homem. A eficiência da toxicidade de Bt incentiva a prospecção de novos isolados em diversas regiões do mundo. Desta forma, em busca de novos isolados de B. thuringiensis potencialmente tóxicos, amostras de solo provenientes dos biomas Amazônia, Cerrado e Caatinga do estado do Maranhão foram avaliadas em relação ao seu potencial larvicida para Aedes aegypti. Os isolados que provocaram elevada toxicidade para larvas do mosquito, detectada por bioensaios, foram avaliados em relação aos potenciais genes responsáveis pela atividade tóxica, além da avaliação de efeitos tóxicos no epitélio intestinal através de análises histológicas em microscopia de luz. Os novos isolados de Bt tóxicos para larva de A. aegypti amplificaram frequências diferentes de genes cry e cyt sendo os mais frequentes (55-33%) os cry4, cyt1, cry32, cry10 e cry11 dentre os investigados. Esses genes codificam para proteínas tóxicas específicas para ordem Diptera, e podem explicar as severas alterações morfológicas provocadas pelas toxinas dos isolados observadas no intestino das larvas de A. aegypti.

Abstract Entomopathogenic agents are viable and effective options due to their selective action against insects but benign effects on humans and the environment. The most promising entomopathogens include subspecies of Bacillus thuringiensis (Bt), which are widely used for the biological control of insects, including mosquito vectors of human pathogens. The efficacy of B. thuringiensis toxicity has led to the search for new potentially toxic isolates in different regions of the world. Therefore, soil samples from the Amazon, Cerrado and Caatinga biomes of the state of Maranhão were evaluated for their potential larvicidal action against Aedes aegypti. The isolates with high toxicity to mosquito larvae, as detected by bioassays, were subjected to histological evaluation under a light microscope to identify the genes potentially responsible for the toxicity. Additionally, the toxic effects of these isolates on the intestinal epithelium were assessed. In the new B. thuringiensis isolates toxic to A. aegypti larvae, cry and cyt genes were amplified at different frequencies, with cry4, cyt1, cry32, cry10 and cry11 being the most frequent (33-55%) among those investigated. These genes encode specific proteins toxic to dipterans and may explain the severe morphological changes in the intestine of A. aegypti larvae caused by the toxins of the isolates.

In this study, the extraction and characterization of aquatic humic substances (AHS) from two blackwater rivers in the Amazon basin was carried out in order to assess the seasonal effects in the AHS structure. In addition, an investigation of the complexing capacities (CC) of the structures containing the metal ions CdII, NiII and PbII was also done. The characterization (of the AHS) was done using UV-Vis, molecular fluorescence, and infrared spectroscopy. AHS from the Negro River showed more aromatic characteristics in its structures and molecular weight, than the AHS from the Carú River. The AHS studied presented a seasonal pattern characterized by higher humification levels during the rainy season. For all the metals, the AHS complexing capacity was higher for the Negro River. The highest complexing capacities were observed for the NiII assays, which were 0.56 and 0.12 µmol NiII mg-1 dissolved organic carbon (DOC), for AHS RN and AHS RC-II, respectively. We concluded that the transport, bioavailability, and toxicity, of these metal species should be higher for Carú River.