Resumen El estudio se realizó en el distrito de Juan Guerra, provincia y región de San Martín, Perú; se evaluaron dos conjuntos de tratamientos: (1) dosis de fertilizante nitrogenado (FN75, FN100); (2) abono verde Crotalaria juncea (CroJ), Canavalia ensiformis (CanE), y sin abono verde. Se dispuso en un diseño de parcela dividida con cuatro repeticiones. Durante el experimento observamos una fluctuación importante en los parámetros del suelo. Notablemente, hubo un decremento en los niveles de carbono y nitrógeno del suelo, comúnmente atribuidos al metabolismo microbiano. Por otra parte, observamos que CanE redujo significativamente los macollos enfermos por el “Virus de la Hoja Blanca” (RHBV) en un 2,82% en comparación con el control, y CroJ logró una fertilidad de panícula significativa (91,88%). No se obtuvieron diferencias significativas en los rendimientos durante esta primera campaña; sin embargo, el mayor rendimiento reportado fue 8,36 t ha-1 con el tratamiento CanE - FN100. Además, la calidad nutricional del arroz no se vio alterada por los abonos verdes o la fertilización química nitrogenada. Estos alcances permiten ahondar en los estudios para considerar alternativas estratégicas para disminuir la dependencia de los fertilizantes inorgánicos por las comunidades más pobres.

Abstract The study was conducted in Juan Guerra district, province and region of San Martin, Peru; it assessed two treatment sets: (1) nitrogen fertilizer dose (FN75, FN100); (2) green manure Crotalaria juncea (CroJ), Canavalia ensiformis (CanE), and without green manure. It was arranged in a split-plot design with four replications. During the experiment, we observed an important fluctuation in soil parameters. Notably, there was a decrease in soil carbon and nitrogen levels, likely attributed to microorganism metabolism. On the other hand, we observed that CanE significantly reduced the diseased tillers through “White Leaf Virus” (RHBV) by 2.82% compared to the control, and significant panicle fertility was achieved by CroJ (91.88%). No significant differences were obtained in yields during this first campaign; however, the highest reported yield was 8.36 t ha-1 with the CanE - FN100 treatment. Additionally, the nutritional quality of the rice was not affected by either green manuring or the application of chemical nitrogen fertilization. These findings allow deeper studies to consider strategic alternatives to reducing dependency on inorganic fertilizers among the poorest communities.

Resumo A exposição solar é responsável pela maior parte das doenças labiais e, quando diagnosticadas tardiamente, o prognóstico é desfavorável, e os tratamentos costumam ser mutilantes, paliativos e onerosos. Este estudo teve como objetivo avaliar a prevalência de doenças labiais e os fatores associados ao seu desenvolvimento em vendedores ambulantes de duas praias do litoral do Guarujá/Brasil. Os dados foram coletados por meio de exame clínico e aplicação de questionário a 182 trabalhadores. Eles também receberam um folder explicativo com informações sobre cuidados bucais e a importância do uso do protetor solar na prevenção do câncer labial. Todos os trabalhadores com diagnóstico de lesão labial foram encaminhados para tratamento odontológico. A prevalência de alterações labiais foi de 40,7% (n = 70); além disso, os fumantes incluíram 27,5% e o consumo de álcool prevaleceu em 20,3% dos participantes. Quanto à proteção individual, 72% dos trabalhadores relataram usar chapéu ou boné com frequência, 48,4% relataram usar protetor solar corporal e apenas 11,5% relataram usar protetor labial. Não houve diferenças significativas em fatores como alcoolismo e tabagismo para o diagnóstico de lesão labial. Porém, ao caracterizar esses fatores, 48,6% dos participantes que bebem ou fumam apresentaram alterações labiais. Pode-se concluir que a população de vendedores ambulantes das praias do Guarujá apresenta alto índice de alteração labial, o que sugere a necessidade de implementação de estratégias educativas em saúde que visem orientar a população sobre os fatores de risco e medidas preventivas das lesões labiais. tardiamente desfavorável mutilantes onerosos GuarujáBrasil Brasil Guarujá/Brasil 18 odontológico 407 40 7 40,7 n 70 70) disso 275 27 5 27,5 203 20 3 20,3 individual 72 frequência 484 48 4 48,4 115 11 11,5 Porém 486 6 48,6 Podese Pode se 1 40, 2 27, 20, 48, 11,

Abstract Sun exposure is responsible for most lip lesions and, when diagnosed late, the prognosis is unfavorable. Treatments are usually mutilating, palliative, and expensive. This study aimed to evaluate the prevalence of lip diseases and factors associated with its development in peddlers on two beaches of the Guarujá/Brazil coast. Data were collected by clinical examination and use of a questionnaire of 182 peddlers on the beaches. They also received an explanatory folder with information about oral care, and the importance of the use of sunscreen for cancer prevention. All workers diagnosed with lip injuries were referred for dental treatment. The prevalence of lip alterations was 40.7% (n = 70), furthermore, smokers included 27.5%, and alcohol consumption was 20.3%. Regarding selfie protection, 72% of workers reported wearing a hat or cap frequently, 48.4% reported using body sunscreen, and only 11.5% reported using lip balm. There were no significant differences in factors such as alcoholism and smoking related to lip lesions diagnosis. However, when characterizing these factors, 48.6% of those who drink, or smoke have lip alterations. It can be concluded that the population of peddlers on the beaches of Guarujá has a high rate of lip alteration which suggests the need to implement educational health strategies aimed at informing the population about risk factors and preventive measures for lip diseases. late unfavorable mutilating palliative expensive GuarujáBrazil Brazil coast 18 care prevention treatment 407 40 7 40.7 n 70, 70 , 70) furthermore 275 27 5 27.5% 203 20 3 20.3% protection 72 frequently 484 48 4 48.4 115 11 11.5 balm diagnosis However 486 6 48.6 drink 1 40. 2 27.5 20.3 48. 11. 27. 20.

Abstract A lectin from the marine sponge Haliclona (Reniera) implexiformis (HiL) was isolated by affinity chromatography on Sepharose™ matrix. HiL showed specificity for galactose and its derivatives. The glycoproteins porcine stomach mucin (PSM) and bovine stomach mucin (BSM) were potent inhibitors. Hemagglutinating activity of the lectin was maximal between pH 5.0 and 9.0. The lectin remained active until 60°C. The presence of CaCl2 and EDTA did not affect the hemagglutinating activity. In SDS-PAGE, HiL showed a single band of 20 kDa under reduced conditions, whereas in the non-reducing conditions, it showed a band of 20 kDa and one additional band of 36 kDa. The average molecular mass determined by Electrospray Ionization Mass Spectrometry (ESI-MS) was 35.874 ± 2 Da in native and non-reducing conditions, whereas carboxyamidomethylated-lectin showed 18,111 Da. These data indicated that HiL consists in a dimer formed by identical subunits linked by disulfide bonds. Partial amino acid sequence of HiL was determined by mass spectrometry, and revealed that it is a new type of lectin, which showed no similarity with any protein. Secondary structure consisted of 6% α-helice, 31% β-sheet, 18% β-turn and 45% random coil. HiL showed significant reduction in the number of viable cells of Staphylococcus biofilms. Reniera (Reniera (HiL Sepharose matrix derivatives PSM (PSM BSM (BSM inhibitors 50 5 0 5. 90 9 9.0 60C C 60 60°C CaCl SDSPAGE, SDSPAGE SDS PAGE, PAGE SDS-PAGE conditions nonreducing non reducing 3 ESIMS ESI MS (ESI-MS 35874 35 874 35.87 carboxyamidomethylatedlectin carboxyamidomethylated 18111 18 111 18,11 bonds spectrometry protein 6 αhelice, αhelice α helice, helice α-helice 31 βsheet, βsheet β sheet, sheet β-sheet βturn turn 45 coil biofilms 9. 3587 87 35.8 1811 1 11 18,1 4 358 8 35. 181 18,

BACKGROUND According to the last 2023 Monkeypox (Mpox) Outbreak Global Map from the Centres for Disease Control and Prevention (CDC), more than 100 countries with no Mpox infection report cases. Brazil stands out in this group and is the second country with the highest number of cases in the last outbreak. OBJECTIVE To contribute to knowledge of the virus infection effects in a cellular model, which is important for diagnosis infections not yet included in a provider´s differential diagnosis and for developing viral inhibition strategies. METHODS We describe a virus isolation protocol for a human clinical sample from a patient from Brazil, the viral growth in a cell model through plaque forming units (PFU) assay, reverse transcriptase polymerase chain reaction (RT-PCR) and transmission electron microscopy (TEM). FINDINGS We follow the viral isolation in Vero cell culture from a Mpox positive clinically diagnosed sample and show the infection effects on cellular structures using a TEM. MAIN CONCLUSIONS Understanding the impact of viral growth on cellular structures and its replication kinetics may offer better strategies for the development of new drugs with antiviral properties. 202 (Mpox CDC, CDC , (CDC) 10 outbreak providers provider s PFU (PFU assay RTPCR RT PCR (RT-PCR TEM . (TEM) properties 20 (CDC 1 (TEM 2

Abstract: The objective of this study was to evaluate the quality of milk and cheese and sensory analysis of cheese from goats that received a diet with different levels of sorghum silage in the diet. Twenty Anglonubian dairy goats were used, with an average age of 2 years, average live weight of 43.7 kg, distributed in a completely randomized design, with five replications. Moisture (P=0.001), total solids (P=0.001), fat (p=0.001), TDE (P=0.006) and protein (P=0.003) of goat cheese showed a significant difference due to the inclusion of silage from goat cheese. sorghum in the diet. The sensory attributes soft appearance (P<.0001), look (P<.0001), salty taste (P<.0001) and smooth texture (P=0.0430) showed a significant difference depending on the level of sorghum silage added to the diet. of goats. In the PC analysis, it is observed that three main components were needed to represent the variation of 64.84% of the data. PC 1 determined 29% of the data variation and was represented by the attributes with the highest averages: attractive aroma (5.87) and global acceptability (7.74). The inclusion of up to 45% of sorghum silage in the diet of goats increased milk and cheese fat, which favored high scores for the attributes of softness and global acceptance. Abstract used years 437 43 7 43. kg design replications P=0.001, P0001 P P=0.001 , 0 001 (P=0.001) p=0.001, p0001 p p=0.001 (p=0.001) P=0.006 P0006 006 (P=0.006 P=0.003 P0003 003 (P=0.003 P<.0001, P<.0001 0001 (P<.0001 P=0.0430 P00430 0430 (P=0.0430 6484 64 84 64.84 29 averages 5.87 587 5 87 (5.87 7.74. 774 7.74 . 74 (7.74) 45 acceptance 4 P000 P=0.00 00 (P=0.001 p000 p=0.00 (p=0.001 (P=0.00 P<.000 000 (P<.000 P=0.043 P0043 043 (P=0.043 648 6 8 64.8 5.8 58 (5.8 77 7.7 (7.74 P00 P=0.0 p00 p=0.0 (p=0.00 (P=0.0 P<.00 (P<.00 P=0.04 P004 04 (P=0.04 64. 5. (5. 7. (7.7 P0 P=0. p0 p=0. (p=0.0 (P=0. P<.0 (P<.0 (5 (7. P=0 p=0 (p=0. (P=0 P<. (P<. ( (7 P= p= (p=0 (P= P< (P< (p= (P (p

RESUMO O reúso de água cinza é uma medida eficaz no enfrentamento da escassez hídrica no semiárido nordestino, tendo em vista que, com sua prática, há diminuição no consumo de água potável para irrigação agrícola. Diante disso, o presente trabalho teve como objetivo avaliar o tratamento de água cinza para reúso em propriedade agrícola familiar no semiárido do Rio Grande do Norte. Para a avaliação do tratamento do efluente, foram realizadas coletas dos esgotos bruto e tratado no vermifiltro da unidade agrícola familiar onde se encontra instalado o projeto piloto utilizado neste trabalho. Foram realizadas análises das propriedades físico-químicas e microbiológicas do efluente, por meio dos parâmetros: temperatura, condutividade elétrica, pH, sólidos suspensos, nitrogênio amoniacal, nitrogênio orgânico, nitrogênio total Kjeldahl (NTK), nitrato, fósforo total, ortofosfato solúvel, demanda bioquímica de oxigênio ao quinto dia (DBO5), demanda química de oxigênio (DQO), oxigênio dissolvido e coliformes termotolerantes. Os resultados indicaram que houve remoção significativa de sólidos suspensos totais, DQO, DBO5 e coliformes termotolerantes. Tendo em vista que a redução dessas concentrações possibilita o reúso agrícola e que o sistema de tratamento biológico utilizado se mostrou eficiente na remoção desses parâmetros, é notório que o tratamento da água cinza constitui ferramenta fundamental na garantia da qualidade de vida da população, especialmente para aqueles que vivem na zona rural e sofrem com a escassez hídrica. Ademais, o sistema apresenta baixo custo de manutenção e operação, diminuição de gastos com fertilizantes, economia de água potável, redução da poluição ambiental e preservação do meio ambiente.

ABSTRACT The reuse of gray water is an effective measure to face the difficulty of irrigation in the northeastern semi-arid region, considering that, with its practice, there is an decrease in the consumption of drinking water for agricultural irrigation. Therefore, the present work aimed to evaluate the treatment of gray water for reuse in a family farm in the semiarid region of Rio Grande do Norte. To assess the treatment of the effluent, raw and treated sewage were collected, evaluated and treated in the vermifilter of the family farm, where the pilot project used in this work is installed. The physical-chemical and microbiological properties of the effluent were elaborated through the parameters: temperature, electrical conductivity, pH, suspended solids, ammonia nitrogen, organic nitrogen, total Kjeldahl nitrogen (NTK), nitrate, total phosphorus, soluble orthophosphate, five-day biochemical oxygen demand (BOD5), chemical oxygen demand (COD), dissolved oxygen and thermotolerant coliforms. The results indicate that there was a significant removal of Total Suspended Solids (TSS), COD, BOD5, and thermotolerant coliforms. Considering that the reduction of these concentrations allows agricultural reuse and that the biological treatment system used proved to be efficient in removing these parameters, it is clear that the treatment of gray water is a fundamental tool in guaranteeing the quality of life of the population, especially for those who live in rural areas and suffer from water scarcity. In addition, the system has low maintenance and operation costs, reduced fertilization costs, savings in potable water, reduced environmental pollution and preservation of the environment.

RESUMO Introdução: o tratamento de fraturas expostas isoladas da diáfise da tíbia (FEIDT) apresenta desafios por frequentemente associar severa lesão óssea com condições ruins de tecido mole, fatores relevantes em países de média e baixa renda, especialmente devido a atrasos na implementação da fixação definitiva e falta de treinamento adequado no manejo de tecidos moles. Consequentemente, FEIDTs representam importante fonte de incapacitação na América Latina. Este estudo objetivou apresentar uma visão geral das FEIDTs em sete hospitais do cone sul da América Latina. O objetivo secundário foi avaliar o seu impacto na qualidade de vida baseado na taxa de retorno ao trabalho (TRT). Métodos: foram incluídos no estudo pacientes com FEIDT tratados em sete hospitais de Brasil e Argentina entre novembro de 2017 e março de 2020. Resultados clínicos e radiográficos foram analisados num período de 120 dias. Avaliação final comparou TRT com o questionário SF-12, consolidação óssea e condições de marcha. Resultados: setenta e dois pacientes foram tratados, 57 seguidos por 120 dias e 48 completaram o questionário SF-12. Após 120 dias, 70,6% havia retornado ao trabalho, 61,4% tinha fratura consolidada. Idade, antibioticoterapia, tipo de tratamento definitivo e infecção influenciaram significativamente na TRT. A condição de marcha apresentou forte correlação com TRT e o componente físico do SF-12. Conclusão: FEIDTs são potencialmente deletérias à qualidade de vida dos pacientes 120 dias após o tratamento inicial. TRT é significativamente maior para pacientes mais jovens, sem história de infecção e que conseguem correr na avaliação da condição de marcha..

ABSTRACT Introduction: open tibial fractures are challenging due to the frequent severe bone injury associated with poor soft tissue conditions. This is relevant in low- and middle-income countries, mainly related to delayed definitive fixation and lack of adequate training in soft tissue coverage procedures. Due to these factors, open tibial fracture is an important source of disability for Latin American countries. Herein we sought to provide an epidemiological overview of isolated open tibial shaft fracture across seven hospitals in southern cone of Latin America. The secondary goal was to assess the impact on quality of life based on return-to-work rate (RWR). Methods: patients with an isolated open tibial shaft fracture treated in seven different hospitals from Brazil and Argentina from November 2017 to March 2020 were included in the study. Clinical and radiographic results were evaluated throughout the 120-day follow-up period. Final evaluation compared RWR with the SF-12 questionnaire, bone healing, and gait status. Results: Seventy-two patients were treated, 57 followed for 120 days and 48 completed the SF-12 questionnaire. After 120 days, 70.6% had returned to work, 61.4% had experienced bone healing. Age, antibiotic therapy, type of definitive treatment, and infection significantly influenced the RWR. Gait status exhibited strong correlations with RWR and SF-12 physical component score. Conclusions: Isolated open tibial shaft fractures are potentially harmful to the patient’s quality of life after 120 days of the initial management. RWR is significantly higher for younger patients, no history of infection, and those who could run in the gait status assessment.

ABSTRACT We aimed to estimate genetic parameters for growth, reproductive, and carcass traits in Tabapuã cattle. Phenotypic data were collected between 1990 and 2019 in 1,218 farms, and the pedigree file had 340,868 animals. The traits evaluated were body weight at 120 (W120), 210 (W210), 365 (W365), and 550 (W550) days of age; age at first calving (AFC), scrotal circumference at 365 days of age (SC365), ribeye area (REA), backfat thickness (BF), and rump fat thickness (RF). The (co)variance components were estimated using the restricted maximum likelihood method, considering single and two-traits animal models. For all traits, the models considered fixed, direct additive genetic, and residual random effects. In addition, for W120 and W210, the maternal additive genetic and maternal permanent environmental effects were also included. Heritabilities for W120, W210, W365, W550, SC365, REA, BF, and RF were of moderate magnitude (0.15, 0.16, 0.23, 0.19, 0.22, 0.36, 0.31, and 0.27, respectively). Low heritability was observed for AFC (0.07). The genetic correlations between growth traits were higher than 0.90, while AFC and SC365 presented negative moderate correlation (−0.66). The REA showed low genetic correlations with BF (0.07) and RF (0.07), whereas BF and RF were highly correlated (0.77). Considering the heritability estimates, selection for AFC would result in limited genetic gain, while for the other traits, it would be satisfactory. Based on the high genetic correlations between growth traits, selection of Tabapuã animals can be performed at younger ages. Additionally, animals can be indirectly selected for AFC through SC365, and only one fat thickness trait may be used in the selection process considering the high genetic correlation and similar heritability values for BF and RF.

Resumo: Introdução: Como o tromboembolismo venoso (TEV) é uma das principais causas de mortes evitáveis em pacientes hospitalizados, é importante a criação de medidas para sua prevenção e seu tratamento. Porém, para a profilaxia de qualidade, a educação e o envolvimento dos pacientes são necessários, e uma das ferramentas para facilitar as atividades educativas em saúde são as tecnologias educacionais. Objetivo: Este estudo teve como objetivos construir e validar um aplicativo móvel sobre orientações para prevenção de TEV em pacientes hospitalizados durante a internação e após a alta hospitalar. Método: Trata-se de um estudo metodológico composto por duas fases (construção e validação), divididas em nove etapas. A fase de validação foi realizada com 11 juízes especialistas e 30 representantes do público-alvo. Utilizaram-se quatro instrumentos de coletas de dados: Suitability Assessment of Materials (SAM), Instrumento de Validação de Conteúdo Educativo em Saúde (IVCES), Instrumento de Avaliação para o Público-alvo e System Usability Scale (SUS), todos interpretados mediante avaliação específica. Resultado: Entre os juízes especialistas, a média da pontuação do SAM foi 89,4%, e a do IVCES, 93,8%. Em relação à opinião do público-alvo, a média de concordância no primeiro instrumento de coleta de dados foi 95%, e a do SUS, 79,5 pontos. Conclusão: O aplicativo móvel foi considerado válido mediante a metodologia aplicada por juízes especialistas e público-alvo.

Abstract: Introduction: Venous thromboembolism (TEV) is one of the main causes of preventable deaths in hospitalized patients. Therefore, it is important to create methods for its prevention and treatment. However, for quality prophylaxis, it is necessary to educate and involve patients, and one of the tools to facilitate educational activities in health is educational technologies. Objective: To build and validate a mobile application on guidelines for preventing TEV in hospitalized patients during hospitalization and after discharge from hospital. Methods: Methodological study consisting of two phases (construction and validation), divided into nine steps. The validation phase was carried out with 11 expert judges and 30 representatives of the target audience. Four data collection instruments were used: Suitability Assessment of Materials (SAM), Health Educational Content Validation Instrument (IVCES), Target Audience Assessment Instrument and System Usability Scale (SUS), all interpreted by specific evaluation. Results: Among the expert judges, the average score for the SAM was 89.4% and for the IVCES, 93.8%. In relation to the opinion of the target audience, the average agreement score in the first data collection instrument was 95% and for the SUS, 79.5 points. Conclusion: The mobile application was considered valid according to the methodology applied by the expert judges and target audience.

RESUMO Inúmeras doenças determinam dano intersticial crônico no parênquima pulmonar e são agrupadas com a denominação de pneumopatias intersticiais fibrosantes, incluindo fibrose pulmonar idiopática, doenças do colágeno, sarcoidose, pneumonite por hipersensibilidade fibrótica etc. Entre os métodos complementares à avaliação clínica, a TCAR tem um papel relevante. Perspectivas atuais de tratamento são encorajadoras e reforçam a necessidade de realização de estudos com técnica adequada, visando a detecção confiável de acometimento intersticial fibrosante o mais precocemente possível. Embora esforços tenham sido direcionados nesse sentido, o significado e manejo de anormalidades pulmonares intersticiais incipientes, detectadas nos estudos de imagem, ainda não são claros. Uma vez detectado o acometimento fibrosante, é importante que o radiologista conheça aspectos característicos de determinados padrões morfológicos e reconheça elementos que possam apontar para entidades clínicas específicas. Em casos nos quais a especificidade dos achados não é suficiente para a suspeição diagnóstica, as imagens de TC servem de guia para a escolha de sítios para biópsia cirúrgica. O seguimento evolutivo é útil para a determinação de pneumopatias fibrosantes progressivas e para a detecção de complicações não relacionadas à doença de base, como infecções, exacerbação aguda e neoplasias. Ferramentas automatizadas de quantificação têm aplicabilidade clínica e devem estar acessíveis para a análise imagética no futuro próximo. Além disso, a incorporação da avaliação tomográfica a escores com parâmetros clínicos e funcionais de estadiamento do acometimento fibrosante poderá se tornar valiosa na determinação prognóstica. O conhecimento das diversas aplicabilidades clínicas do método tomográfico é fundamental aos especialistas que acompanham esses pacientes, podendo impactar positivamente sua trajetória clínica.

ABSTRACT Many conditions result in chronic interstitial lung disease (ILD), being classified as fibrosing ILDs, including idiopathic pulmonary fibrosis, connective tissue diseases, sarcoidosis, and fibrotic hypersensitivity pneumonitis. HRCT plays an important role in the clinical evaluation of fibrosing ILDs. Current treatment perspectives are encouraging and reinforce the need for HRCT scans of adequate technical quality for early detection of fibrosing ILD. Despite efforts in this regard, the significance and management of imaging findings of early interstitial lung abnormalities have yet to be clarified. After identification of CT findings consistent with fibrosing ILD, radiologists must be able to identify characteristic morphological patterns and, in some cases, features of specific clinical entities. In cases in which HRCT features are not sufficiently specific for a definitive diagnosis, HRCT can aid in selecting the best site for surgical lung biopsy. CT follow-up is useful for identifying progressive fibrosing ILDs and detecting complications unrelated to the underlying disease, including infections, acute exacerbations, and neoplasms. Automated quantification tools have clinical applicability and are likely to be available for use in imaging analysis in the near future. In addition, incorporation of CT evaluation into scoring systems based on clinical and functional parameters for staging fibrosing disease is likely to become valuable in determining prognosis. Knowledge of the clinical applications of CT evaluation is essential for specialists managing patients with fibrosing ILD and can have a positive impact on the clinical course of the disease.

Abstract The aim of the present study was to isolate, select and characterize endophytic bacteria in rice inhibiting Burkholderia glumae THT as well as to characterize the genetic diversity and virulence factors in strains of B. glumae and Burkholderia gladioli of rice. Rice plants were collected in 4 departments from the northern region of Peru, isolating endophytic bacteria, aftertissue sterilization, at 30°C (48 h) in Trypticase SoyAgar (TSA), evaluating the antimicrobial activity against B. glumae THT, production of siderophores, resistance of toxoflavine and partial sequencing of the 16S rRNA gene. Furthermore, B. glumae and B. gladioli were isola-ted in selective medium (pH 4.5) at 41 °C/72h. Molecular identification was performed using BOX-PCR and sequencing of the 16S rRNA gene, in addition to the production of extracellular enzymes, motility tests and sensitivity/resistance to bactericides. One hundred and eighty nine (189) endophytic bacteria were isolated, and only 9 strains showed antimicrobial activity against B. glumae THT, highlighting Burkholderia vietnamiensis TUR04-01, B. vietnamiensis TUR04-03 and Bacillus aryabhattai AMH12-02. The strains produced siderophores and at least 55.5% were resistant to toxoflavin. Additionally, 17 strains were grouped into 9 BOX-PCR profiles, where 16 had similarity with B. glumae LMG2196T (100%) and 1 with B. gladioli NBRC 13700T (99.86%). High diversity was found according to geographical origin and virulence factors. In conclusion, strains of the genus Bacillus and Burkholderia are potential biocontrol agents against B. glumae.

Resumen La demanda de xilanasas fúngicas en los procesos biotecnológicos industriales muestra un claro aumento en todo el mundo, por lo que hay un interés en ajustar las condicionesde producción de xilanasas microbianas. En este estudio se optimizó la capacidad del hongoFusarium solani para producir xilanasas extracelulares con escasa actividad celulolítica medi-ante el dise˜no de Box-Wilson. Se determinaron las mejores condiciones de cultivo para obteneruna preparación enzimática cruda con una actividad xilanolítica significativa y poca actividad celulolítica. En la mayoría de los tratamientos, la actividad xilanolítica fue mayor que laactividad celulolítica. Se observó un efecto negativo sobre la producción de endoxilanasas, xilosidasas y endocelulasas con el aumento de la concentración de xilano. El aumento del tiempode incubación afectó adversamente la producción de endocelulasas y xilosidasas. De acuerdocon el modelo matemático y las pruebas experimentales, es posible producir endoxilanasas conuna actividad endocelulasa mínima aumentando el tiempo de incubación y la concentración desulfato de amonio. Las condiciones de cultivo óptimas para producir una mayor cantidad deendoxilanasas (10,65 U/mg) y mínima cantidad de endocelulasas fueron 2,5% (p/v) de xilano y5, 2 y 0,4 g/l de extracto de levadura, sulfato de amonio y urea, respectivamente, con 120 hde incubación.

Resumen Se realizó un ensayo clínico aleatorizado con el objetivo de analizar los efectos de un programa de prevención de recaídas basado en Mindfulness para el tratamiento del tabaquismo. El estudio se realizó en el Centro de Atención Psicosocial del Alcohol y las Drogas (CAPSad), en 2017, en la ciudad de Porto Velho, capital de Rondônia, Brasil. Participaron en la investigación 52 pacientes, de ambos sexos, con edades comprendi-das entre 29 y 60 años, consumidores de tabaco solamente. Para la realización de este trabajo, se formaron dos grupos: G1 = experimental y G2 = control, formados por 26 personas cada uno. En la prueba previa, los datos mostraron que no había diferencia significativa entre los grupos. En la comparación de los resultados en la prueba pos-terior se halló una diferencia significativa en la reducción de los síntomas de ansiedad (p = 0.043) y en el nível de monóxido de carbono-CO2 (p = 0.03) em los pulmones de los participantes. Como resultado del análisis de proporciones, se presentó una diferencia en el G1 en la prueba posterior, con síntomas disminuidos de ansiedad (p = <0.001), depresión (p = 0.012) y monóxido de carbono (<0.001), respectivamente. Estos hallazgos mostraron los efectos significativos del mindfulness para la pre-vención de recaídas y reducción de los síntomas de ansiedad, depresión y nível de monóxido de carbono em los pulmones, en el grupo de experimento posterior a la intervención en fumadores y fumadores dependientes que reciben tratamiento en el CAPSad.

Abstract A randomized clinical trial was conducted to analyze the effects of a Mindful-ness-based relapse prevention program for tobacco dependence treatment. The study was conducted at the Center for Psychosocial Care of Alcohol and Drugs (CAPSad), in the city of Porto Velho, capital of Rondônia, Brazil, 2017. 52 patients aged between 29 to 60, of both sexes, who were only tobacco users, participated in the research. To carry out this study, two groups were made up: G1 = experimental and G2 = control, both consisted of 26 people. In the pre-test, the data showed that there was no significant difference between these two groups. In the post-test comparison, there was a considerable difference in the reduction of anxiety symptoms (p = 0.043) and carbon monoxide-CO2 level (p = 0.03) in the lungs of smoking patients. In the result of the proportion analysis, there was a difference in G1 post-intervention with decreased symptoms of anxiety (p = <0.001), depression (p = 0.012) and carbon monoxide (p = <0.001), respectively. These fin-dings showed significant effects of mindfulness in preventing relapse and reducing symptoms of anxiety, depression and carbon monoxide level the post-intervention group of smokers and dependent smokers treated at CAPSad.

Resumo Realizou-se um ensaio clínico randomizado com o objetivo de analisar os efeitos de um programa de Prevenção de Recaídas baseado em mindfulness no tratamento do tabagismo. O estudo foi conduzido no Centro de Atenção Psicossocial de Álcool e Drogas (CAPSad) em 2017 na cidade de Porto Velho, capital de Rondônia, Brasil. Participaram da pesquisa 52 pacientes em tratamento para o tabagismo, com idades entre 29 a 60 anos de ambos os sexos, usuários apenas de tabaco. Para a realização deste trabalho foram formados dois grupos: G1=experimental e G2=controle, ambos formados por 26 cada um. No pré-teste, os dados mostraram não haver diferença significativa entre esses dois grupos. Na comparação do pós-teste ocorreu uma diferença significativa na diminuição dos sintomas de ansiedade (p = 0,043) e nível de monóxido de carbono-CO2 (p = 0,03) nos pulmões dos participantes. No resultado da análise de proporção, houve diferença no G1 pós-intervenção com diminuição de sintomas de ansiedade (p == <0,001), depressão (p = 0,012) e monóxido de carbono (p = <0,001), respectivamente. Estes achados mostraram efeitos significativos do mindfulness para a pre- venção de recaídas e diminuição de sintomas de ansiedade, depressão e nível de monóxido de carbono nos pulmões, no G1 pós-intervenção nos pacientes fumantes e fumantes dependentes em tratamento no CAPSad.