Abstract Sanionia uncinata, or Sickle-leaved-Hook-moss, is a cosmopolitan pleurocarpous moss composing the Antarctic Peninsulae biodiversity, primordially forming dense mats over rocks. The species was collected in 24 different spots located at King George Island and was processed to obtain 24 ethanolic extracts (ADS#) by a serial-24h-maceration, which were prospected for antimicrobial, cytotoxic, antioxidant, and acetylcholinesterase (AChE) inhibition activities by using in vitro tests. Alien material was removed from the non-sterilized plant samples before being submitted for extraction. It was observed that extracts collected in different spots showed different biological activities. Extracts ADS04(10.66±0,17mm) and ADS14(11.37±0,11mm) were active against Staphylococcus aureus, according to the diffusion in bioautography assay. They showed significant antioxidant activity and inhibited AChE; the cytotoxicity observed to the human breast cancer cells MCF-7 and MDA-MB-231 were higher than in normal cell line MCF-10A. ADS04 was 7.62 times more cytotoxic to MCF-7, and ADS14 was 2.03 times more cytotoxic to MDA-MB-231 than to MCF-10A. The extracts showed similar cytotoxicity between PC-3, a human prostate cancer cell line, and MCF-10A. Sanionia uncinata extracts are a vital potential source of biologically active compounds, particularly ADS04 and ADS14, including further prospection on eventual bryophyte’s endophytic fungi. SickleleavedHookmoss, SickleleavedHookmoss Sickle leaved Hook moss, Sickle-leaved-Hook-moss biodiversity rocks 2 ADS# ADS (ADS# serial24hmaceration, serial24hmaceration serialhmaceration serial 24h maceration, maceration h serial-24h-maceration antimicrobial AChE (AChE tests nonsterilized non sterilized extraction ADS0410.66±0,17mm ADS041066017mm ADSmm 10.66±0,17mm 10 66 0 17mm mm ADS04(10.66±0,17mm ADS1411.37±0,11mm ADS141137011mm 11.37±0,11mm 11 37 11mm ADS14(11.37±0,11mm aureus assay MCF7 MCF 7 MCF- MDAMB231 MDAMB MDA MB 231 MDA-MB-23 MCF10A. MCF10A MCFA 10A. 10A A MCF-10A ADS0 762 62 7.6 MCF7, 7, ADS1 203 03 2.0 PC3, PC3 PC 3, 3 PC-3 compounds bryophytes bryophyte s fungi (ADS hmaceration ADS0410 1066017mm 1 6 ADS1411 1137011mm MDAMB23 23 MDA-MB-2 76 7. 20 2. PC- ADS041 ADS141 MDAMB2 MDA-MB- MDA-MB

RESUMO O objetivo desta pesquisa foi avaliar os SNPs rs471462296, rs456245081 e rs438495570 do gene DGAT1 em bovinos Nelore. Foram analisados 109 bovinos. A extração do DNA genômico foi realizada do sangue dos animais, usando-se o kit Ilustra Blood Genomic Prep Mini Spin® (GE Healthcare, UK). A concentração e o grau de pureza do DNA foram determinados por meio de espectrofotômetro (Nanodrop - Thermo Fisher Scientifc, USA). A genotipagem dos SNPs ocorreu mediante o emprego do ensaio Taqman® (Applied Biosystems, USA). Na análise genômica, não foram encontradas alterações nas frequências alélicas e genotípicas (P≥0,05) para os SNPs testados. Dessa forma, a região 5’UTR analisada apresentou-se monomórfica e a variação de SNPs não foi observada, o que limita seu uso como marcadores moleculares para o gene DGAT1 em Nelore.