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This study aimed to isolate, purify and characterize secondary metabolites present in Amburana acreana leaves, native to the state of Rondônia, using classical chromatographic methods and 1D, 2D nuclear magnetic resonance spectroscopy. From the hydroethanolic leaves extract of A. acreana, p-hydroxybenzoic acid, vanillic acid, coumarin, campesterol 3-β-D-glucoside and amburoside B, all known compounds, were identified. To the best of our knowledge, this is the first report of isolation and chemical characterization of secondary metabolites in A. acreana. In addition, in vitro assays indicated that amburoside B does not have inhibitory potential on the growth of gram-negative bacteria. The presence of these compounds suggests that, thus far, amburosides (A and B) could be chemotaxonomic markers of the genus Amburana. isolate Rondônia 1D D spectroscopy A phydroxybenzoic p hydroxybenzoic acid coumarin 3βDglucoside βDglucoside 3 β glucoside identified knowledge addition gramnegative gram negative bacteria far

https://doi.org/10.21577/0100-4042.20250010

Chemical constituents, antioxidant and antimicrobial activities of Pavonia glazioviana Gurke (Malvaceae) constituents Malvaceae (Malvaceae
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Oliveira, Micaelly S.

; Chaves, Otemberg S.

; Teles, Yanna C. F.

; Fernandes, Diégina A.

; Silva, Camila Macaúbas

; Queiroz, Wallace A. M.

; Lima, Jéssica B.

; Mazzotti, Maristela R. R. M.

; Lima, Edeltrudes O.

; Fernandes, Givanildo L.

; Conceição, Adilva S.

; Souza, Maria de Fátima Vanderlei de

Química Nova

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2025, Volume 48 N. 1 elocation e-20250011

Abstract: En | Text: En | PDF: En

Species from Pavonia genus (Malvaceae) are traditionally used as antioxidant, n anti inflammatory and antimicrobial remedies. Previous studies have revealed their diversity of specialized metabolites widely used in the development of new medicines. This is the first study on Pavonia glazioviana Gurke, a Brazilian species known as “Malva da Chapada”. The research led to identify fifteen compounds: 132 (S) hydroxy pheophytin a; and 132(S) hydroxy 173 ethoxyphaeophorbide, n decanol, cycloart 23Z ene 3β,25 diol, cycloart 24S 25 ene 3β,24 diol, sitosterol 3 O β D glucopyranoside, stigmasterol 3 O β D glucopyranoside, quercetin, acacetin, kaempferol, tiliroside, 5 hydroxy 3,7,8,4’ tetramethoxyflavone, 5,7 dihydroxy 3,8,4’ trimethoxyflavone, 5,7 dihydroxy 4’ methoxyflavone, 5,7,4’ trihydroxy 3,8 dimethoxyflavone and 5,7,4’ trihydroxy 3 methoxyflavone. The major flavonoids were tested for their antimicrobial activity, and the compound 5,7 dihydroxy 3,8,4’ trimethoxyflavone showed strong activity against Escherichia coli (MIC = 512 μg mL1), Pseudomonas aeruginosa (MIC = 512 μg mL1), Candida albicans (MIC = 512 μg mL1), Candida tropicalis (MIC = 512 μg mL1), Candida parapsilosis (MIC = 512 μg mL1), Aspergillus flavus (MIC = 512 μg mL1) and A. fumigantus (MIC = 512 μg mL1). The total phenolic content, flavonoid content, and radical scavenging potential were determined showing its great production of phenolics and flavonoids, along with interesting radical scavenging potential (DPPH EC50 = 6.36 ± 0.029 mg mL 1). Our findings contributed to characterize P. glazioviana as a source of methoxylated flavonoids with antimicrobial potential. Malvaceae (Malvaceae antioxidant remedies medicines Gurke Malva Chapada. Chapada . Chapada” compounds 13 S (S 132S 132(S 17 ethoxyphaeophorbide decanol Z 3β25 3β 3β,2 diol 2 3β24 24 glucopyranoside quercetin acacetin kaempferol tiliroside 3,7,8,4 3784 7 8 4 tetramethoxyflavone 57 5, 3,8,4 384 methoxyflavone 5,7,4 574 38 3, MIC 51 mL1, mL1 , A mL1. content DPPH EC EC5 636 6 36 6.3 0029 0 029 0.02 1. 1 1) P 3β2 3β, 3,7,8, 378 3,8, 5,7, 63 6. 002 02 0.0 3,7,8 37 00 0. 3,7, 3,7

https://doi.org/10.21577/0100-4042.20250011

Ultra-Low-Dose Computed Tomography for Pneumonia in the Emergency Department: A Feasibility Study
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Bianco, Danilo P.

; Accorsi, Tarso A. D.

; Souza, Jose L.

; Mello, Eduardo S.

; Silva, Joselito A.

; Teles, Gustavo B. S.

; Chate, Rodrigo C.

; Santos, Andre R. C.

; Moron, Renato A.

; Mattos, Fernando R.

; Teles, Adriana Gusman

; Oliveira, Roger Pereira

; Almeida, Elizabeth Aparecida

; Zimmer, Paulo M.

; Funari, Marcelo B. G.

; Benveniste, Marcelo

; Szarf, Gilberto

Acta Radiológica Portuguesa

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apr 2024, Volume 36 N. 1 Pages 12 - 17

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução: O papel da tomografia computorizada torácica de ultrabaixa dose (ULDCT) em contexto de urgência é desconhecido. Objetivo: Este estudo avaliou a viabilidade da ULDCT em adultos na urgência com suspeita de pneumonia. Foram medidos a dose de radiação, o tempo entre a solicitação e os relatórios do exame, prescrições de antimicrobianos também foram correlacionadas. Métodos: Este estudo avaliou a viabilidade da ULDCT em adultos na urgência com suspeita de pneumonia. Foram medidas a dose de radiação, o tempo entre a solicitação e a disponibilização dos relatórios do exame, e também foram correlacionadas as prescrições de antimicrobianos. Entre outubro de 2017 e dezembro de 2018, incluímos prospectivamente adultos com suspeita de pneumonia adquirida na comunidade, sem diagnóstico definitivo por avaliação clínica. Os critérios de exclusão abrangeram condições clínicas que pudessem afetar a interpretação da imagem. Resultados: Foram incluídos 131 pacientes elegíveis (idade média de 43,5 anos; 57% homens) encaminhados para ULDCT. Dezoito (13,7%) foram excluídos. A dose média de radiação da ULDCT foi de 0,14 mSv. O intervalo de tempo entre a solicitação médica e o resultado da ULDCT foi de 43 minutos. Conclusão: A ULDCT mostrou-se viável para pacientes adultos com suspeita de pneumonia em contexto de urgência. Os relatórios foram disponibilizados num intervalo razoável e os resultados negativos foram associados a uma baixa taxa de prescrição de antibióticos.

Abstract Background: The role of ultra-low-dose chest CT (ULDCT) in the emergency department is unknown. Purpose: This study sought to examine feasibility of ULDCT in adults presenting to the emergency department for whom pneumonia was suspected. Radiation dose and time interval between medical request and ULDCT results were measured, antibiotic prescriptions were correlated. Methods: Between October 2017 and December 2018, we prospectively enrolled consecutive healthy adult patients in a single-center for whom community-acquired pneumonia was suspected and for whom no definitive diagnosis was possible by clinical judgment. Exclusion criteria comprised all chronic conditions that could impact severity and interpretation of image. Results: 131 eligible patients (mean age 43.5 years; 57% men) were enrolled to ULDCT. Eighteen (13.7%) patients were excluded. Average CT radiation dose was 0.14 mSv. Time interval between medical request and ultra-low-dose chest CT result was 43 minutes. Only 15.9% of patients with negative CT received antibiotics. Conclusion: Ultra-low-dose chest CT was feasible for almost all patients in a group of healthy adults presenting to the emergency department with suspected community-acquired pneumonia. Results were made available in a reasonable interval, and negative ones were associated with a low antibiotic prescription rate.

https://doi.org/10.25748/arp.33024

CIRCUITO ESPACIAL DA PRODUÇÃO DE CALÇADOS E INTERAÇÕES TERRITORIAIS DA EMPRESA GRENDENE, SOBRAL, CE GRENDENE SOBRAL
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Teles, G. A.

; Costa, M. P. S.

Mercator (Fortaleza)

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2023, Volume 22 elocation e22002

Abstract: Es Pt En | Text: Es Pt En | PDF: Es | PDF: Pt | PDF: En

Resumen Para la industria del calzado, Sobral, en Ceará, se destaca como el territorio productivo más expresivo, hecho que se justifica por la ubicación de las unidades productivas de Grendene. Este artículo buscó analizar los aspectos generales de las interacciones territoriales en la producción de calzado de Grendene-Sobral a partir de los circuitos espaciales de producción. Para llevar a cabo esta demanda, los procedimientos metodológicos consistieron en: a) revisión bibliográfica a partir de autores especializados en el tema; b) levantamiento documental y estadístico yc) investigación de campo. A través de la investigación, se constató, entre otros resultados, que Sobral recibe todas las órdenes y determinaciones de producción, caracterizándose como un territorio utilizado para la producción propia. Por lo tanto, el producto es diseñado en el Sur, producido en Sobral y distribuido al mercado consumidor nacional y mundial, lo que explica la multiescalaridad de insumos, la organización del proceso productivo y la multiescalaridad de distribución y consumo de los productos de la empresa. producción de calzado. Ceará expresivo Grendene GrendeneSobral demanda tema b yc campo constató resultados propia tanto Sur mundial insumos empresa

Resumo Para a indústria calçadista, Sobral, no Ceará, se destaca como o mais expressivo território produtivo, fato que se justifica pela localização de unidades produtivas da Grendene. Este artigo buscou analisar os aspectos gerais das interações territoriais da produção de calçados da Grendene-Sobral com base nos circuitos espaciais da produção. Para a realização desta demanda, os procedimentos metodológicos consistiram em: a) revisão de literatura arrimada em autores especialistas no tema; b) levantamento documental e estatístico e c) pesquisa de campo. Por intermédio da pesquisa, constatou-se, entre outros resultados, que Sobral recebe todas as ordens e determinações da produção, caracterizando-se como um território usado para a produção propriamente dita. Assim sendo, o produto é idealizado no Sul, produzido em Sobral e distribuído para o mercado consumidor nacional e global, o que explica a multiescalaridade dos insumos, a organização do processo produtivo e a multiescalaridade da distribuição e consumo da produção calçadista da empresa. Ceará Grendene GrendeneSobral demanda tema b c campo constatouse, constatouse constatou se, constatou-se resultados caracterizandose caracterizando dita sendo Sul global insumos empresa

Abstract For the footwear industry, Sobral, in Ceará, stands out as the most expressive productive territory, a fact that is justified by the location of Grendene's production units. This article sought to analyze the general aspects of territorial interactions in the production of footwear by Grendene-Sobral based on the spatial circuits of production. In order to carry out this demand, the methodological procedures consisted of: a) literature review based on authors specialized in the subject; b) documentary and statistical survey and c) field research. Through the research, it was found, among other results, that Sobral receives all orders and determinations of production, characterizing itself as a territory used for production itself. Therefore, the product is designed in the South, produced in Sobral and distributed to the national and global consumer market, which explains the multi-scalarity of inputs, the organization of the production process and the multi-scalarity of distribution and consumption of the company's footwear production. industry Ceará Grendenes Grendene s units GrendeneSobral demand subject b c research found results Therefore South market multiscalarity multi scalarity inputs companys company

https://doi.org/10.4215/rm2023.e22002

Suicide attempt, impulsivity, and exposure to trauma in college students
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Lira, Sidnei B.

; Vieira, Flávia

; Cavalcanti, Diogo E.

; Souza-Marques, Breno

; Netto, Liana R.

; Correia-Melo, Fernanda S.

; Leal, Gustavo C.

; Pereira, Juliana L.

; Santos, Lene L.

; Guedes, Gisela M.

; Teles, Carlos A.

; Cardoso, Taiane de A.

; Miranda-Scippa, Ângela

; Kapczinski, Flavio

; Lacerda, Acioly L.T.

; Koenen, Karestan C.

; Turecki, Gustavo

; Quarantini, Lucas C.

Brazilian Journal of Psychiatry

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jun 2022, Volume 44 N. 3 Pages 279 - 288

Abstract: En | Text: En | PDF: En

Objectives: Past suicide attempt (SA) is one of the most important risk factors for suicide death. An ideation-to-action framework posits that impulsivity, potentially traumatic events, and mental disorders also play a role in increasing suicide risk. This study aimed to assess the association between trait impulsivity, lifetime exposure to trauma, and post-traumatic stress disorder (PTSD) with SA in a sample of Brazilian college students. Methods: A total of 2,137 participants filled self-reported questionnaires consisting of a sociodemographic and clinical questionnaire, Trauma History Questionnaire, Post-Traumatic Stress Disorder Checklist – Civilian version, and Barratt Impulsiveness Scale. Results: Our findings suggest that trait impulsivity may be interpreted as exerting a distal effect on SA, even in the presence of other variables – such as trauma history, psychological neglect, and PTSD – which also increase the odds of SA. High and medium levels of impulsivity, history of trauma, and PTSD increased the likelihood of SA. Conclusions: Intervention strategies to prevent SA may target trait impulsivity and exposure to traumatic experiences.

https://doi.org/10.1590/1516-4446-2021-2175

Natural and Semisynthetic Triterpenes from Combretum leprosum Mart. with Antiplasmodial Activity
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Passarini, Guilherme M.

; Ferreira, Amália S.

; Moreira-Dill, Leandro S.

; Zanchi, Fernando B.

; Jesus, Aurileya G. de

; Facundo, Valdir A.

; Teles, Carolina B. G.

Journal of the Brazilian Chemical Society

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may 2022, Volume 33 N. 5 Pages 483 - 490

Abstract: En | Text: En | PDF: En

Malaria is responsible for thousands of deaths each year. Currently, artemisinin combination therapy (ACT) is used as first-choice medication against the disease. However, the emergence of resistant strains prompts the search for alternative compounds. The present study aimed to investigate the antiplasmodial activities of natural triterpenes (compounds 1 and 2), and semisynthetic derivatives 1a, 1b, 1c, and 1d. Antiplasmodial assays were carried out using the SYBR Green technique, whereas cytotoxicity was evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. Hemolytic assays were performed on human erythrocytes. An in silico analysis of the compounds against PfENR (Plasmodium falciparum 2-trans-enoyl-reductase) was carried out by molecular docking. Experiments with 1, and its derivatives against P. falciparum showed that 1a was very similar in terms of biological activity to compound 1 (half maximal inhibitory concentration (IC50) ca. 4 µM), whereas 1b, 1c, and 1d had reduced antiplasmodial activities (IC50 between 8-103 µM). The selectivity indexes of 1 and 1d for HepG2, and Vero cells were > 10. Docking results partially agreed with the in vitro experiments, with 1 and 1c having the best and worst affinities with PfENR, respectively. In conclusion, the results showed that 1 and 1d may serve as biotechnological tools in the development of antimalarial drugs.

https://doi.org/10.21577/0103-5053.20210167

COVID-19 in the Perioperative Period of Cardiovascular Surgery: the Brazilian Experience
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Gomes, Walter J.

; Rocco, Isadora

; Pimentel, Wallace S.

; Pinheiro, Aislan H. B.

; Souza, Paulo M. S.

; Costa, Luiz A. A.

; Teixeira, Marjory M. P.

; Ohashi, Leonardo P.

; Bublitz, Caroline

; Begot, Isis

; Moreira, Rita Simone L

; Hossne Jr, Nelson A.

; Vargas, Guilherme F.

; Branco, João Nelson R.

; Teles, Carlos A.

; Medeiros, Eduardo A. S.

; Sáfadi, Camila

; Rampinelli, Amândio

; Moratelli Neto, Leopoldo

; Rosado, Anderson Rosa

; Mesacasa, Franciele Kuhn

; Capriata, Ismael Escobar

; Segalote, Rodrigo Coelho

; Palmieri, Deborah Louize da Rocha Vianna

; Jardim, Amanda Cristina Mendes

; Vianna, Diego Sarty

; Coutinho, Joaquim Henrique de Souza Aguiar

; Jazbik, João Carlos

; Coutinho, Henrique Madureira da Rocha

; Kikuta, Gustavo

; Almeida, Zely Sant'Anna Marotti de

; Feguri, Gibran Roder

; Lima, Paulo Ruiz Lucio de

; Franco, Anna Carolina

; Borges, Danilo de Cerqueira

; Cruz, Felipe Ramos Honorato De La

; Croti, Ulisses Alexandre

; Borim, Bruna Cury

; Marchi, Carlos Henrique De

; Goraieb, Lilian

; Postigo, Karolyne Barroca Sanches

; Jucá, Fabiano Gonçalves

; Oliveira, Fátima Rosane de Almeida

; Souza, Rafael Bezerra de

; Zilli, Alexandre Cabral

; Mas, Raul Gaston Sanchez

; Bettiati Junior, Luiz Carlos

; Tranchesi, Ricardo

; Bertini Jr, Ayrton

; Franco, Leandro Vieira

; Fernandes, Priscila

; Oliveira, Fabiana

; Moraes Jr, Roberto

; Araújo, Thiago Cavalcanti Vila Nova de

; Braga, Otávio Penna

; Pedrosa Sobrinho, Antônio Cavalcanti

; Teixeira, Roberta Tavares Barreto

; Camboim, Irla Lavor Lucena

; Gomes, Eduardo Nascimento

; Reis, Pedro Horigushi

; Garcia, Luara Piovan

; Scorsioni, Nelson Henrique Goes

; Lago, Roberto

; Guizilini, Solange

Brazilian Journal of Cardiovascular Surgery

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dec 2021, Volume 36 N. 6 Pages 725 - 735

Abstract: En | Text: En | PDF: En

Abstract Introduction: We investigated the clinical course and outcomes of patients submitted to cardiovascular surgery in Brazil and who had developed symptoms/signs of coronavirus disease 2019 (COVID-19) in the perioperative period. Methods: A retrospective multicenter study including 104 patients who were allocated in three groups according to time of positive real time reverse transcriptase-polymerase chain reaction (RT-PCR) for the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2): group 1, patients who underwent cardiac surgery > 10 days after positive RT-PCR; group 2, patients with a positive RT-PCR within 10 days before or after surgery; group 3, patients who presented positive RT-PCR > 10 days after surgery. The primary outcome was mortality and secondary outcomes were postoperative complications, intensive care unit (ICU) length of stay, and postoperative days of hospitalization. Results: The three groups were similar with respect to age, the European System of Cardiac Operative Risk Evaluation score, and comorbidities, except hypertension. Postoperative complications and death were significantly higher in groups 2 and 3 than in group 1, and no significant difference between groups 2 and 3 was seen. Group 2 showed a high prevalence of surgery performed as an urgent procedure. Although no significant differences were observed in ICU length of stay, total postoperative hospitalization time was significantly higher in group 3 than in groups 1 and 2. Conclusion: COVID-19 affecting the postoperative period of patients who underwent cardiovascular surgery is associated with a higher rate of morbidity and mortality. Delaying procedures in RT-PCR-positive patients may help reduce risks of perioperative complications and death.

https://doi.org/10.21470/1678-9741-2021-0960

FATTY ACIDS ANALYSIS AND CHEMOTAXONOMIC CONSIDERATIONS OF MALVOIDEAE (MALVACEAE) SPECIES
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Fernandes, Diégina A.

; Chaves, Otemberg S.

; Teles, Yanna C. F.

; Agra, Maria de F.

; Vieira, Maria A. R.

; Silva, Paulo S. S. da

; Marques, Marcia O. M.

; Souza, Maria de Fátima Vanderlei de

Química Nova

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feb 2021, Volume 44 N. 2 Pages 137 - 141

Abstract: En | Text: En | PDF: En

Previous researches showed that fatty acids analysis might be a useful tool to support the taxonomic investigation. In this approach the fatty acids content of ten Malvoideae species was analyzed and its chemotaxonomic significance has been investigated. The aerial parts of the species were collected in the Northeast of Brazil and their fatty acid methyl esters were analyzed by gas chromatography with flame ionization detector. The chemometric analysis consisted of principal component analysis (PCA) and hierarchical clustering analysis (HCA) with the euclidean distance between the samples given by the Ward.D2 algorithm. This is the first report of fatty acids from Wissadula peripocifolia, Herissantia crispa, Bakeridesia pickelii, Sidastrum micranthum, Pavonia cancellata and Pavonia malacophylla. The results showed the predominance of palmitic (C16:0), oleic (C18:1) and linoleic (C18:2) acids in the studied species. By the PCA and HCA analysis, the fatty acid composition was able to distinguish the species Herissantia crispa and Pavonia malacophylla. Our findings showed a chemotaxonomic proximity among species from different genera reflecting the taxonomic and phylogenetic closeness previously demonstrated by molecular investigations on Malvoidae species. Furthermore, our results demonstrated that the fatty acid analysis may be an interesting tool to support the taxonomic investigations on Malvoideae species.

https://doi.org/10.21577/0100-4042.20170668 125 downloads

Characterization of proteinuria in treated and untreated dogs naturally infected with Leishmania sp.
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Valle, Pillar G.

; Veado, Júlio C.C.

; Ribeiro, Vitor M.

; Teles, Pedro P.A.

; Val, Adriane P. Costa

; Dornelas, Lorraine R.S.M.

; Fonseca, Leandro A.

; Leme, Fabiola O. Paes

Pesquisa Veterinária Brasileira

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2021, Volume 41 elocation e06905

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Na busca por um biomarcador precoce de injúria renal, este trabalho teve como objetivo determinar o perfil proteico urinário de cães infectados com leishmaniose sem tratamento e tratados conforme determina a legislação brasileira. A identificação da proteinúria, sua classificação e as circunstâncias em que ocorrem instigaram este estudo. Para tanto, foram avaliados 30 cães oriundos do atendimento clínico ambulatorial de um Hospital Veterinário em Belo Horizonte. Todos os animais passaram por exame clínico e laboratorial, que incluíram biomarcadores renais. As proteínas foram caracterizadas através da técnica de eletroforese por SDS-PAGE, e assim, foi elaborado um perfil proteico urinário comparando pacientes considerados clinicamente hígidos, com cães infectados por Leishmania (L.) infantum e que estavam sob tratamento e cães infectados não tratados. Os resultados demonstraram que os parâmetros hematológicos e bioquímicos apresentaram comportamento semelhante entre os grupos de cães hígidos e de cães infectados com L. infantum tratados, entretanto um padrão muito heterogêneo de proteínas urinárias pode ser observado e diferiu entre animais hígidos e animais com leishmaniose, assim como entre os animais tratados e não tratados. Os resultados sugerem que a classificação da proteinúria pode ser uma ferramenta que auxilia no estadiamento de animais infectados por L. infantum podendo diferenciá-los quanto à gravidade de lesões renais existentes.

ABSTRACT: In the search for an early biomarker of renal injury, this study aimed to determine the urinary protein profile of dogs with leishmaniasis without treatment and treated as determined by Brazilian legislation. The identification of proteinuria, its classification and the circumstances in which it takes place instigated this study. For this, 30 dogs from an outpatient clinic at a Veterinary Hospital in Belo Horizonte were evaluated. All animals underwent clinical and laboratory tests, which included renal biomarkers. The proteins were characterized using the SDS-page electrophoresis technique, and thus, a urinary protein profile was developed comparing patients considered clinically healthy with dogs infected with leishmaniasis that were under treatment and with untreated infected dogs. The results showed that the hematological and biochemical parameters showed similar behavior between the groups of healthy dogs and dogs with leishmaniasis treated, however a very heterogeneous pattern of urinary proteins can be observed and differed between healthy animals and animals with leishmaniasis, as well as between treated and untreated animals. The results suggest that the classification of proteinuria can be a tool that helps in the staging of animals infected with L. infantum and can differentiate them as to the severity of existing kidney injuries.

https://doi.org/10.1590/1678-5150-pvb-6905

10.

Targeted massively parallel sequencing for congenital generalized lipodystrophy
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Costa-Riquetto, Aline D.

; Santana, Lucas S.

; Caetano, Lílian A.

; Lerário, Antônio M.

; Correia-Deur, Joya E. M.

; Bertola, Débora R.

; Kim, Chong A.

; Nery, Márcia

; Jorge, Alexander A. L.

; Teles, Milena G.

Archives of Endocrinology and Metabolism

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oct 2020, Volume 64 N. 5 Pages 559 - 566

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: Our aim is to establish genetic diagnosis of congenital generalized lipodystrophy (CGL) using targeted massively parallel sequencing (MPS), also known as next-generation sequencing (NGS). Subjects and methods: Nine unrelated individuals with a clinical diagnosis of CGL were recruited. We used a customized panel to capture genes related to genetic lipodystrophies. DNA libraries were generated, sequenced using the Illumina MiSeq, and bioinformatics analysis was performed. Results: An accurate genetic diagnosis was stated for all nine patients. Four had pathogenic variants in AGPAT2 and three in BSCL2. Three large homozygous deletions in AGPAT2 were identified by copy-number variant analysis. Conclusions: Although we have found allelic variants in only 2 genes related to CGL, the panel was able to identify different variants including deletions that would have been missed by Sanger sequencing. We believe that MPS is a valuable tool for the genetic diagnosis of multi-genes related diseases, including CGL.

https://doi.org/10.20945/2359-3997000000278 1067 downloads

11.

Phytochemical study of Waltheria viscosissima and evaluation of its larvicidal activity against Aedes aegypti
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Ferreira, Maria Denise Leite

; Fernandes, Diégina A.

; Nunes, Fabíola C.

; Teles, Yanna C.F.

; Rolim, Yngred M.

; Silva, Camila Macaúbas da

; Albuquerque, Janderson B.L. de

; Agra, Maria de Fátima

; Souza, Maria de F.V. de

Revista Brasileira de Farmacognosia

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oct 2019, Volume 29 N. 5 Pages 582 - 590

Abstract: En | Text: En | PDF: En

Abstract The species Waltheria viscosissima A.St.–Hil, Malvaceae, which is known as ‘malva-branca', is traditionally used in the Brazilian northeast for the treatment of coughs. This research looks towards reporting the isolation of phytoconstituents of W. viscosissima, as well as the quantification of its phenolics, total flavonoid content, and free radical scavenging potential, along with an evaluation of its larvicidal activity against Aedes aegypti larvae. Chromatographic techniques were used to isolate the compounds and a structural elucidation was performed by 1D and 2D NMR. The quantification of total phenolics and flavonoids and the DPPH˙ radical scavenging activity was determined through spectrophotometric methods. Consequently, the phytochemical investigation led to the identification of fourteen compounds from the aerial parts of the W. viscosissima: steroids, triterpenes, alkaloids, and eight flavonoids previously reported in the literature. The quantification of compounds showed that the aerial parts extract possessed high concentration of flavonoids, while the roots extract were rich in other phenolic compounds. At the DPPH˙ free radical scavenging assay, the roots extract presented EC50 = 77.32 ± 4.37 µg/ml and the aerial parts extracts showed EC50 = 118.10 ± 1.21 µg/ml. W. viscosissima roots extract showed the most potent larvicidal activity against Ae. aegypti (LC50 = 4.78 mg/ml), with the potential of being used in effective and economically viable preparations that can be catered for domestic use towards controlling the vector insect of severe diseases, such as dengue and Zika.

https://doi.org/10.1016/j.bjp.2019.05.008 616 downloads

12.

Hepatitis B virus infection among oncohematologic disease patients in Central Brazil: prevalence, risk factors and immunization
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Pessoni, Grécia C.

; Marinho, Tássia A.

; Carneiro, Megmar M. Santos

; Martins, Regina M.

; Soares, Caroline C.

; Silva, Leandro N.

; Matos, Marcia A.

; Arantes, Adriano M.

; Teles, Juliana A.

; Santos, Nathalia C.

; Teles, Sheila Araujo

Hematology, Transfusion and Cell Therapy

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sep 2019, Volume 41 N. 3 Pages 199 - 204

Abstract: En | Text: En | PDF: En

ABSTRACT Introduction: Carriers of oncohematological diseases are at high risk for hepatitis B virus (HBV) infection. Objective and method: To investigate the epidemiology of HBV infection in Goiânia, Central Brazil, 322 individuals with oncohematological diseases (leukemias, Hodgkin lymphoma and non-Hodgkin lymphoma) were interviewed and blood samples were collected for the detection of serological markers of HBV-DNA by polymerase chain reaction (PCR). Medical records of participants were also reviewed. Results: Non-Hodgkin's lymphomas (n = 99) and chronic myeloid leukemia (n = 108) were the most frequent oncohematological diseases. The overall prevalence of HBV was 13.97% (45/322). Of the total participants, 8.69% (28/322) presented isolated positivity for anti-HBs, suggesting low vaccine coverage. HBV-DNA was detected in 25% (1/4) of HBsAg positive samples and in 25% (3/12) of anti-HBc isolated, suggesting HBV occult infection. All samples were identified as subgenotype A1. Entries in patient records and the findings of this investigation suggest anti-HBc seroconversion during oncologic treatment. Age 50 years or over and use of a central catheter during therapy were associated with HBV exposure. Conclusion: The low frequency of hepatitis B immunized individuals, detection of HBV DNA in HBsAg negative samples, and the suggestion of HBV exposure during treatment evidenced the potential for health-related viral dissemination in people with oncohematological diseases in our region, reinforcing the importance of serological monitoring, vaccination against hepatitis B, and adoption of strict infection control measures in these individuals.

https://doi.org/10.1016/j.htct.2018.11.008 672 downloads

13.

Searching for mutations in the HNF1B gene in a Brazilian cohort with renal cysts and hyperglycemia
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Dotto, Renata P.

; Santana, Lucas Santos de

; Lindsey, Susan C.

; Caetano, Lilian Araújo

; Franco, Luciana F.

; Moisés, Regina Célia M. S.

; Sa, João R.

; Nishiura, José Luiz

; Teles, Milena Gurgel

; Heilberg, Ita P.

; Dias-da-Silva, Magnus R.

; Giuffrida, Fernando M. A.

; Reis, André F.

Archives of Endocrinology and Metabolism

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jun 2019, Volume 63 N. 3 Pages 250 - 257

Abstract: En | Text: En | PDF: En

ABSTRACT Objective To verify the presence of variants in HNF1B in a sample of the Brazilian population selected according to the presence of renal cysts associated with hyperglycemia. Subjects and methods We evaluated 28 unrelated patients with clinical suspicion of HNF1B mutation because of the concomitant presence of diabetes mellitus (DM) or prediabetes and renal cysts. Genotyping was accomplished using Sanger sequencing or multiplex ligation-dependent probe amplification (MLPA). In positive cases, available relatives were recruited. Results We found two patients with HNF1B mutations. The first presented the variant p.Pro328Leufs*48(c.983delC) and had DM, renal cysts, and hypomagnesemia. The second presented a heterozygous whole gene deletion in HNF1B, DM, renal cysts, body and tail pancreatic agenesis, and hypomagnesemia; this alteration was also found in his two siblings and his father. Conclusion The recruitment of suspected cases of HNF1B gene mutations in Brazilians due to hyperglycemia and renal cysts presents two positive cases. Our cases contribute to the annotation of clinical and biochemical phenotypes of this rare form of maturity-onset diabetes of the young (MODY).

https://doi.org/10.20945/2359-3997000000138 1160 downloads

14.

Applicability of the Platelia EIA® Aspergillus test for the diagnosis of aspergilosis in penguins
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Cabana, A. L.

; Xavier, M. O.

; Mendes, J. F.

; Teles, A. J.

; Martins, A. M.

; Silva-Filho, R. P.

; Meireles, M. C. A.

Brazilian Journal of Biology

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apr 2019, Volume 79 N. 2 Pages 169 - 173

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Ainda hoje, um teste diagnóstico eficaz para aspergilose em pinguins não é conhecido, sendo o padrão-ouro os exames post-mortem. O antígeno fúngico galactomanana (GM) tem sido utilizado como biomarcador da doença em humanos, sendo detectado pelo Platelia Aspergillus EIA (BioRad)®, um kit comercial que se baseia na técnica ELISA sanduíche. É padronizado para utilização em pacientes neutropênicos, no entanto estudos tem demonstrado sua possível utilidade também para aves.O objetivo de nosso estudo foi avaliar a eficácia do teste Platelia Aspergillus EIA® (BioRad-US) no diagnóstico da aspergilose em pinguins-de-Magalhães, determinando sensibilidade, especificidade e valores preditivos positivos e negativos em diferentes pontos de corte. Foram incluídas no estudo, amostras de soro sanguíneo (n=29) de pinguins-de-Magalhães em cativeiro que vieram a óbito por aspergilose. A detecção de GM foi realizada seguindo instruções do fabricante e o índice de GM foi obtido dividindo o valor da média da DO da duplicata da amostra clínica pelo valor da média da DO da duplicata da amostra de cut-off fornecida pelo kit. Através de informações em banco de dados foram obtidos resultados sobre a presença de anticorpos anti-Aspergillus fumigatus, detectada por Imunodifusão em gel de ágar (IDGA) em todas as amostras séricas. Os resultados foram analisados utilizando-se teste de qui-quadrado e Kruskal-Wallis a partir do programa estatístico SPSS 20.0, IBM®. Curva ROC foi obtida e a partir desta, taxas de sensibilidade, especificidade, valores preditivo positivo e negativo foram igualmente calculados considerando quatro diferentes pontos de corte (0.5, 1.0, 1.5 e 2.0). O índice de GM sérica não diferiu entre os animais do grupo caso e controle (pKW = 0.097). Na determinação da curva ROC para detecção de GM sérica o valor da área sobre a curva foi de 0.635. A partir dos valores determinados pelas coordenadas da curva, quatro diferentes pontos de corte (0.5, 1.0, 1.5 e 2.0) foram analisados, resultando em taxas de sensibilidade variando de 86.2% a 34.5%, e de especificidade entre 87% e 26.1%. Ao comparar o índice de GM sérica nos animais do grupo caso quanto a presença ou não de anticorpos detectados pela IDGA foi encontrado p=0.503. A detecção de GM pelo teste Platelia Aspergillus EIA® não parece ser útil para o diagnóstico da aspergilose em pinguins naturalmente infectados.

Abstract Even today, an effective diagnostic test for aspergillosis in penguins is unknown, being the gold standard post-mortem examinations. The fungal antigen galactomannan (GM) has been used as a biomarker of disease in humans and is detected by the Platelia Aspergillus EIA (BioRad)®, a commercial kit based on the sandwich ELISA technique. It is standardized for use in neutropenic patients, however studies have demonstrated its usefulness also possible for birds. The aim of our study was to evaluate the effectiveness of Platelia Aspergillus EIA® test (BioRad-US) in the diagnosis of aspergillosis in Magellanic penguins, determining sensitivity, specificity, and positive and negative predictive values for different cut-off points. Were included in the study, blood serum samples (n = 29) Magellanic penguins in captivity that died by aspergillosis. Detection of GM was performed following manufacturer's instructions and the GM index was obtained by dividing the average value of OD of the duplicate of the clinical sample by duplicate OD of the average value of the cut-off sample provided by the kit. Through information database results were obtained for the presence of anti-Aspergillus fumigatus antibodies detected by agar gel immunodiffusion (AGID) for all serum samples. Results were analyzed using chi-square test and Kruskal-Wallis from SPSS 20.0, IBM®. ROC curve was obtained and from this, rates of sensitivity, specificity, positive and negative predictive values were also calculated based on four different cutoff points (0.5, 1.0, 1.5 and 2.0). The serum GM index did not differ between animals of the case and control group (pkw =0.097). In determining the ROC curve for serum GM detection the value of area under the curve was 0.635. From the values determined by the coordinate of the curve, four different cut points (0.5, 1.0, 1.5 and 2.0) were analyzed, resulting in sensitivity rates ranging from 86.2 to 34.5% % and specificity between 87% and 26.1%. By comparing the serum GM index in group case as the presence or absence of antibodies detected by AGID was found p=0.503. The detection of GM the Platelia Aspergillus EIA® test seems is not be useful for the diagnosis of aspergillosis in naturally infected penguins.

https://doi.org/10.1590/1519-6984.171140 829 downloads

15.

Nematodes associated with Iguana iguana (Linnaeus, 1758) (Squamata, Iguanidae) in Semi-arid areas of Northeastern Brazil
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Teles, D. A.

; Brito, S. V.

; Teixeira, A. A. M.

; Ribeiro, S. C.

; Araujo-Filho, J. A.

; Lima, V. F.

; Pereira, A. M. A.

; Almeida, W. O.

Brazilian Journal of Biology

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jul 2017, Volume 77 N. 3 Pages 514 - 518

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Foram analisados os nematóides em Iguana iguana, um grande lagarto Iguanidae que possui ampla distribuição pelas Américas. Os objetivos do estudo foram investigar os helmintos associados ao lagarto I. iguana e seus índices parasitológicos (prevalência e intensidade média de infecção). Foram examinados 18 espécimes (oito machos e 10 fêmeas). A prevalência total foi de 66,6% (12/18), nos machos foi de 75% (6/8) e nas fêmeas 60% (6/10). Iguana iguana foi identificado como um novo hospedeiro para Physaloptera sp., Atractis sp., Piratuba sp., e Subulura sp. Este registro contribui para o conhecimento da diversidade de helmintos associados a este hospedeiro.

Abstract Nematodes were analyzed in Iguana iguana, a large lizard Iguanidae that is widely distributed throughout the Americas. The aims of the study were investigate the helminths associated with the lizard, I. iguana in semi-arid areas of northeastern Brazil and analyze the parasitological indices (prevalence, and mean intensity of infection). A total of 18 specimens of I. iguana were examined (8 males and 10 females). The overall prevalence of infection was 66.6% (12/18), while in males, it was 75% (6/8) and in females, 60% (6/10). Iguana iguana was identified as a new host for Physaloptera sp., Atractis sp., Piratuba sp. and Subulura sp. This registered contributes to the knowledge of helminth diversity associated with this host.

https://doi.org/10.1590/1519-6984.17615 1499 downloads

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