Resumo: A quinoa foi reconhecida como a única “cultura nutricional abrangente”; no entanto, é suscetível à germinação na pré-colheita (BHS). Embora a reação em cadeia da polimerase com transcrição reversa quantitativa (RT-qPCR) tenha sido amplamente empregada para detecção do nível de expressão gênica, a seleção de genes de referência adequados é essencial para garantir a quantificação precisa da expressão gênica em diversas condições. Este estudo tem como objetivo identificar genes de referência estáveis em sementes de quinoa tratadas com ABA e GA, a fim de fornecer uma base para pesquisas subsequentes em BPC. As sementes foram submetidas a diferentes concentrações de ABA e GA (10 μM, 50 μM, 100 μM e 200 μM). A concentração de tratamento mais adequada foi determinada com base na viabilidade das sementes. MON1, GAPDH, EIF3, EF1α, ACT, TUB1 e TUB6 foram selecionados como genes candidatos. A adequação destes genes de referência sob diferentes condições foi avaliada utilizando vários métodos, incluindo valores Ct, geNorm, NormFinder, BestKeeper, Delta Ct e RefFinder. Com base nos resultados obtidos nos experimentos com hormônios, observou-se que a aplicação de 100 μM de ABA e 200 μM de GA produziu os resultados mais vantajosos. Além disso, os genes de referência mais apropriados para diferentes tratamentos são ACT e TUB1 (tratamento com H2O), EIF3 e MON1 (tratamento com ABA, GA e para o conjunto de dados combinados dos três grupos). No entanto, o GAPDH exibiu a menor estabilidade em todos os tratamentos. Em resumo, o ACT é recomendado como gene de referência para a germinação natural da quinoa, enquanto o EIF3 e o MON1 devem ser utilizados para os tratamentos com ABA e GA. Resumo cultura abrangente abrangente” entanto précolheita pré colheita BHS. BHS . (BHS) RTqPCR RT qPCR (RT-qPCR BPC 10 (1 5 20 μM. μM) MON EIF EF1α EFα EF α TUB candidatos métodos geNorm NormFinder BestKeeper RefFinder hormônios observouse observou se vantajosos disso H2O, H2O HO , H O H2O) grupos. grupos grupos) resumo (BHS 1 ( 2

Abstract: Quinoa has been recognized as the sole “comprehensive nutritional crop”; however, it is susceptible to pre-harvest sprouting (PHS). While quantitative reverse transcription polymerase chain reaction (RT-qPCR) has been extensively employed for gene expression level detection, the selection of suitable reference genes is imperative to ensure precise gene expression quantification across diverse conditions. This study aims to identify stable reference genes in quinoa seeds under ABA and GA, in order to provide a basis for subsequent research on PHS. Seeds were subjected to different concentrations of ABA and GA (10 μM, 50 μM, 100 μM, and 200 μM). The most suitable treatment concentration was determined based on seed viability. Here, MON1, GAPDH, EIF3, EF1α, ACT, TUB1, and TUB6 were selected as candidate genes. The suitability of these reference genes under different conditions was assessed using various methods including Ct values, geNorm, NormFinder, BestKeeper, Delta Ct, and RefFinder. Based on the results obtained from the hormone experiments, it was observed that the application of 100 μM ABA and 200 μM GA yielded the most advantageous outcomes. Additionally, the most appropriate reference genes for different treatments are ACT and TUB1 (H2O treatment), EIF3 and MON1 (ABA, GA treatment and also for the combined data set of the three groups). However, GAPDH exhibited the least stability across all treatments. In summary, ACT is recommended as the reference gene for natural quinoa germination, while EIF3 and MON1 should be used for ABA and GA treatments. Abstract comprehensive crop crop” however preharvest pre harvest PHS . (PHS) RTqPCR RT qPCR (RT-qPCR detection 10 (1 5 20 μM. μM) viability Here MON EIF EF1α EFα EF α TUB values geNorm NormFinder BestKeeper RefFinder experiments outcomes Additionally H2O HO H O treatment, , treatment) ABA, (ABA groups. groups groups) However summary germination (PHS 1 ( 2

ABSTRACT This experiment was conducted to investigate the effects of lactic acid bacteria preparations on microbial diversity and community structure of calves. On days 1 and 7 of the trial period, feces were collected into sterile tubes and labeled (Day 1: control group D1DZ, experimental group D1SY, and Day 7: control group D7DZ, experimental group D7SY). Twenty Angus calves (150±10 kg) were selected and randomly divided into two groups of 10 calves each. The control group fed a basal diet. In addition to feeding the basal diet, the experimental group was given 15 mL lactobacillus preparation orally at 09:00 and 16:00 h every day. Calves were allowed free feeding and drinking water. All other feeding environments and management conditions remained consistent with the experiment lasting for seven days. At the end of the experiment, the fecal microflora of the calves was analyzed using 16S rRNA sequencing techniques. The 16S rRNA analysis data were processed using the Excel 2007 software and analyzed by the IBM SPSS statistical software (Statistical Analysis System, version 22). The Alpha diversity index analysis showed that the Chao and the Ace indices were significantly different after feeding supplemented with lactic acid bacteria. The PCA analysis showed that the fecal flora structure differed significantly after supplementation with the lactic acid bacteria preparation. Further analysis showed that the lactic acid bacteria increased Firmicutes, Patescibacteria, Rikenellaceae_RC9_gut_group, Clostridium_sensu_stricto_1, and prevotellaceae_UCG-003 in the feces. Therefore, we speculate that lactic acid bacteria preparations play an important role in animal production and are beneficial to the diversity of the fecal microflora of the calves. period D1DZ DDZ D DZ D1SY DSY SY D7DZ D7SY. D7SY . D7SY) 150±10 15010 150 (150±1 kg each diet 0900 09 00 09:0 1600 16 16:0 day water S techniques 200 Statistical System 22. 22 22) Firmicutes Patescibacteria RikenellaceaeRC9gutgroup RikenellaceaeRCgutgroup Rikenellaceae RC9 gut RC Rikenellaceae_RC9_gut_group Clostridiumsensustricto1 Clostridiumsensustricto Clostridium sensu stricto Clostridium_sensu_stricto_1 prevotellaceae_UCG003 prevotellaceaeUCG003 prevotellaceaeUCG prevotellaceae_UCG 003 prevotellaceae UCG prevotellaceae_UCG-00 Therefore 150±1 1501 (150± 090 0 09: 160 16: 20 2 RikenellaceaeRC gutgroup Clostridium_sensu_stricto_ UCG003 prevotellaceae_UCG00 prevotellaceaeUCG00 prevotellaceae_UCG-0 150± (150 Clostridium_sensu_stricto UCG00 prevotellaceae_UCG0 prevotellaceaeUCG0 prevotellaceae_UCG- (15 UCG0 (1 (

Abstract Objective: To explore the clinical or sociodemographic predictors for both successful and failed extubation among Chinese extremely and very preterm infants Methods: A retrospective cohort study was carried out among extremely and very preterm infants born at less than 32 weeks of gestational age (GA). Results: Compared with the infants who experienced extubation failure, the successful infants had higher birth weight (OR 0.997; CI 0.996-0.998), higher GA (OR 0.582; 95% CI 0.499-0.678), a caesarean section delivery (OR 0.598; 95% CI 0.380-0.939), a higher five-minute Apgar score (OR 0.501; 95% CI 0.257-0.977), and a higher pH prior to extubation (OR 0.008; 95% CI 0.001-0.058). Failed extubation was associated with older mothers (OR 1.055; 95% CI 1.013-1.099), infants intubated in the delivery room (OR 2.820; 95% CI 1.742-4.563), a higher fraction of inspired oxygen (FiO2) prior to extubation (OR 5.246; 95% CI 2.540-10.835), higher partial pressure of carbon dioxide (PCO2) prior to extubation (OR 7.820; 95% CI 3.725-16.420), and higher amounts of lactic acid (OR 1.478;95% CI1.063-2.056). Conclusions: Higher GA, higher pre-extubation pH, lower pre-extubation FiO2 and PCO, and lower age at extubation are significant predictors of successful extubation among extremely and very preterm infants.

ABSTRACT At present, most large machinery and vehicle engines are electronically controlled but their electronic control systems are too expensive to be popularised and applied in small gasoline engines with relatively low prices. Therefore, small gasoline engines still use mechanical speed regulators. Mechanical speed regulators not only have the defects of inertia lag, friction resistance, inherent speed regulation and the like, but also have the disadvantage that dynamic performance and steady performance cannot be combined, which is not suitable for the increasingly improved speed regulation performance of gasoline engines. This paper describes the design of an electronically controlled intelligent governor for small gasoline engines. Starting from a low cost, it adopts the idea of “replacing part of the hardware with an intelligent sensing algorithm” and proposes an intelligent sensing algorithm scheme. It combines the “coarse tuning” of MAP and the “fine tuning” of adaptive expert PID. The system is proved experimentally and it not only overcomes the inherent defects of a mechanical governor and realises programmable speed regulation, but it also obtains good dynamic and steady performance, improves the power output of the engine, relieves the trouble of engine travelling and improves the freedom of carburetor matching.

The present study aimed to investigate the in vivo inhibitory effect of histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) combined with sorafenib on human hepatocellular carcinoma HCCLM3 cells. The nude mice transplanted with HCCLM3 cells were randomly divided into control, SAHA, sorafenib and SAHA+sorafenib groups. The nude mice in the later 3 groups were intragastrically administrated with SAHA (10 mg·kg-1·day-1), sorafenib (10 mg·kg-1·day-1) and SAHA (10 mg·kg-1·day-1) combined with sorafenib (10 mg·kg-1·day-1), respectively, for successive 20 days. Finally, the inhibition rate of tumor was measured. The expressions of MEK1/2, p-ERK1/2, Cyclin D1, Bcl-2, Bax, p53, MMP-2, MMP-9 and uPA in tumor tissues were determined. Results showed that, compared with SAHA and Sorafenib groups, in SAHA+sorafenib groups the inhibition rate of tumor was significantly increased (P < 0.05), the expression levels of MEK1/2, p-ERK1/2, Cyclin D1, Bcl-2, MMP-2 and MMP-9 and uPA protein in tumor tissues were significantly decreased, respectively (P < 0.05), and the expression levels of Bax and p53 protein were significantly increased, respectively (P < 0.05). In conclusion, compared with single drug, SAHA combined with sorafenib can enhance the inhibitory effects on HCCLM3 xenografts in nude mice.

Objective: To examine the psychometric properties of the Chinese version of the Yale-Brown Obsessive Compulsive Scale – Second Edition (Y-BOCS-II). Method: A total of 86 adults with a primary diagnosis of obsessive-compulsive disorder (OCD), ranging in age from 15 to 78 years, participated in the study. Participants were administered the Y-BOCS-II by a trained clinician who also rated overall illness severity on two additional measures. Patients completed the Obsessive Compulsive Inventory-Revised and Depression Anxiety Stress Scale-21. Results: Results indicated high internal consistency and fair 1-week test retest reliability. The Y-BOCS-II scales correlated strongly with clinician-rated obsessive-compulsive severity and modestly with self-reported obsessive-compulsive symptom frequency and distress. The relationship between Y-BOCS-II total score and depressive and anxiety symptoms was strong, which may reflect high rates of comorbid conditions in this sample or the linkage between obsessive-compulsive symptom severity and distress. Factor analysis demonstrated a two-factor structure consisting of obsession and compulsion factors, with interference due to obsessions cross-loading. Conclusions: Overall, these results support the use of the Y-BOCS-II among individuals from China. Future study by an independent group is necessary to replicate these findings, as well as investigate interrater reliability and treatment sensitivity.

Two new coordination polymers [Zn (bdc)(bpybzimH2)](DMF)0.5 (1, H2bdc=1,4-dicarboxybenzene, bpybzimH2=6,6′-bis-(1H-benzoimidazol-2-yl)-2,2′-bipyridine, DMF=N,N-dimethylformamide) and [Co (bpybzimH2)(sbc)]H2O (2, H2sbc=4-mercaptobenzoic acid) have been successfully prepared under solvothermal conditions using the multi-N chelating organic ligand bpybzimH2 as the foundational building block. In addition, the Cell Counting Kit-8 assay was conducted to evaluate the anti-proliferation activity of compounds 1 and 2 against human spinal tumor cells OPM-2. The cell viability curves showed that the two compounds have anti-proliferation activity on spinal tumor cells, and the activity of compound 1 is higher than compound 2. The annexin V-FITC/PI assay and western blot were used to detect the apoptotic percentage of OPM-2 cells incubated with compounds 1 and 2. The YAP protein expression and its role in cell apoptosis were further studied with qRT-PCR, immunoblotting, and flow cytometer.

Abstract This study investigated the effects of puerarin (PUE) on blood lipid and inflammatory factor levels in rats with lower limb arteriosclerosis obliterans (ASO). Sixty rats were randomly divided into control, model, simvastatin, low-PUE, middle-PUE and high-dose PUE group. The animals in later 5 groups were with lower limb ASO, and the later 4 groups were given 1 mg/kg simvastatin and 5, 10 and 20 mg/kg PUE, respectively. The blood lipid and inflammatory factor levels were determined. Results showed that, the serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and high-sensitivity C-reactive protein (hs-CRP) level in model group were significantly increased (P <0.01), while the high-density lipoprotein cholesterol (HDL-C) was significantly decreased (P <0.01). Compared with model group, TC, TG, LDL-C, IL-6, TNF-α and hs-CRP in high-dose PUE and simvastatin group were significantly decreased (P <0.01 or P <0.05), and HDL level was significantly increased (P <0.01 or P <0.05). There was no significant difference of each index between simvastatin and high-dose PUE group (P >0.05). PUE can obviously decrease the blood lipid and inflammatory factor levels in rats with lower limb ASO.

Abstract: Background: The clinical significance of anti-neutrophil cytoplasmic antibodies in patients with new-onset systemic lupus erythematosus, especially in systemic disease accompanied by interstitial lung disease remains to be elucidated. Objectives: This study was designed to investigate the role of anti-neutrophil cytoplasmic antibodies in new-onset systemic lupus erythematosus patients. Methods: A hundred and seven patients with new-onset SLE were enrolled. Presence of anti-neutrophil cytoplasmic antibodies in the sera was assessed by indirect immunofluorescence as well as enzyme linked immunosorbent assay against proteinase-3 and myeloperoxidase. Clinical features and laboratory parameters of patients were also recorded. All patients were subjected to chest X-ray, chest high-resolution computed tomography and pulmonary function test. Results: Forty-five systemic lupus erythematosus patients (45/107, 42%) were seropositive for anti-neutrophil cytoplasmic antibodies. Compared with anti-neutrophil cytoplasmic antibodies-negative patients, the anti-neutrophil cytoplasmic antibodies-positive patients had significantly higher incidence of renal involvement, anemia, and Raynaud's phenomenon as well as decreased serum level of complement 3/complement 4 and elevated erythrocyte sedimentation rate. In addition, there was a positive correlation between serum anti-neutrophil cytoplasmic antibodies level and disease activity of systemic lupus erythematosus. Furthermore, prevalence of interstitial lung disease in the anti-neutrophil cytoplasmic antibodies -positive patients (25/45, 55.6%) was obviously higher than that in the anti-neutrophil cytoplasmic antibodies-negative patients (15/62, 24.2%). Study limitations: The sample size was limited and the criteria for screening new-onset systemic lupus erythematosus patients might produce bias. Conclusions: The level of anti-neutrophil cytoplasmic antibodies in new-onset systemic lupus erythematosus patients correlates positively with the disease activity and the prevalence of interstitial lung disease.

Abstract Background: Familial progressive hyper- and hypopigmentation (FPHH) is a rare genodermatosis that is characterized by diffuse hyper- and hypopigmented spots on the skin and mucous membranes. It is caused by a pathogenic mutation of the KITLG gene. Objectives: To investigate the clinical features and mutation of the KITLG gene in a Chinese family with FPHH. Methods: Histopathological and immunohistochemical analysis of lesions from the proband was performed. The KITLG gene was screened for the presence of mutations. Results: A Chinese family containing 14 individuals with FPHH was described, and the proband was a 5-year-old girl showing diffuse hyper- and hypopigmented lesions on her extremities and trunk. Histopathological and immunohistochemical staining for S100 and HMB45 of skin biopsy specimens from the hyperpigmented areas showed a striking increase in melanin throughout the epidermis, especially in the basal cell layer, and staining of hypopigmented area specimens displayed lower levels of melanin in the epidermis. Mutation analysis of the KITLG gene was performed, but no mutation was found. Study limitations: The new pathogenic gene was not found. Conclusion: A family with FPHH was described. Analysis revealed that its members did not have any mutations of the KITLG gene, which provided evidence for genetic heterogeneity of this genodermatosis.

Giant magneto-impedance (GMI) effect and domain transformation for melt-extracted Co68.15Fe4.35Si12.25B13.25Nb1Cu1 amorphous wires have been induced by a cryogenic Joule annealing (CJA) treatment with different DC current amplitude (0~350 mA) for 240s. Experimental results indicate that the maximum GMI ratio ([ΔΖ∕Ζ0]max) achieves to 188.1% with exciting field increasing to 1.8 Oe monotonically for 300 mA annealing treated wires, which can attribute to the surface complex domain structure change formed by CJA treatment. The liquid nitrogen can protect the wire from crystallization when applied large DC currents. Moreover, the CJA treatment can improve the response sensitivities effectively. These remarkable characteristics make the melt-extracted microwires by CJA tailoring as the promising candidate material for small-sized magnetic sensors.

A sensitive and stable electrochemical sensor based on polyaniline/graphene-ferrocenecarboxylic acid (PANI/GR-FCA) composite film modified glass carbon electrode for determination of nitrite was presented and its morphology and structure were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR) and X-ray powder diffraction (XRD). Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were used to investigate the electrochemical behavior of the nitrite oxidation, which exhibited an enhanced voltammetric response at the modified electrode. In the optimal condition, the oxidation of nitrite in Britton-Robinson buffer solution (BR) of pH 4.0 had the higher peak current than the bare electrode. A linear calibration curve over the concentration range 0.4-300 µmol L-1 of nitrite were obtained with a linear regression equation I (µA) = 0.1589 + 0.0020C (µmol L-1) (R = 0.9991, n = 13) and the detection limit was down to 0.02 mmol L-1 (S/N = 3). The recoveries of all determinations ranged between 96-102%. At the same time, the modified electrode showed a good long-term stability and reproducibility for determination of nitrite, as well as high selectivity.

Background Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not from Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and investigate its expression during adipocyte differentiation. Results We have isolated a 7654 bp fragment from Peking duck adipocytes that corresponds to the FAS gene. The cloned fragment contains an open reading frame of 7545 bp, encodes a 2515 amino acid protein, and displays high nucleotide and amino acid homology to avian FAS orthologs. Twelve hour treatment of oleic acid significantly up-regulated the expression of FAS in duck preadipocytes (P < 0.05). However, 1000 µM treatment of oleic acid exhibited lipotoxic effect on cell viability (P < 0.05). In addition, during the first 24 h of duck adipocyte differentiation FAS was induced; however, after 24 h its expression level declined (P < 0.05). Conclusion We have successfully cloned and characterized Peking duck FAS. FAS was induced during adipocyte differentiation and by oleic acid treatment. These findings suggest that Peking duck FAS plays a similar role to mammalian FAS during adipocyte differentiation.

Dois novos alcaloides tipo-licopódio, acetil-licoposerramina M e palcernina A foram isolados de extratos de plantas inteiras de Palhinhaea cernua L. juntamente com dez compostos previamente identificados. As estruturas dos dois novos compostos foram elucidadas por métodos espectroscópicos e difratometria de Raios X de monocristal usando o parâmetro Flack.

Two new Lycopodium alkaloids, acetyllycoposerramine M and palcernine A were isolated from whole plant extracts of Palhinhaea cernua L. together with ten previously identified compounds. The structures of the new compounds were elucidated by spectroscopic methods and single-crystal X-ray diffraction analyses using the Flack parameter.