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ABSTRACT Veterinary drug residues in bulk tank milk are important to all sectors of the dairy chain because they are one of the major factors which determine the safety of the final product. This study attempted to identify milk quality parameters that are associated with the occurrence of veterinary drug residues using multivariate principal component analysis (PCA). A total of 132 raw milk samples were collected from 45 dairy farms in the state of Minas Gerais - Brazil and analyzed for 42 analytes, including pyrethroids, macrocyclic lactones and antibacterials, using liquid chromatography coupled with mass spectrometry in tandem mode and gas chromatography with electron capture detection. Out of the 132 milk samples, 40 samples tested positive for at least one analyte (above the detection limit). The milk parameters associated with the antimicrobial residues by confirmatory tests were lactose and nonfat concentrations, as revealed by PCA. This analysis showed that fat and total solid concentrations, as well as the somatic cell and total bacteria counts were associated with macrocyclic lactone residues in bulk tank milk. A PCA assessing pyrethroid residues in bulk tank milk revealed that the lactose and nonfat solid concentrations and titratable acidity were inversely associated with these residues. Thus, the data analysis indicated that the veterinary drug residues were associated with certain milk quality parameters that can be used to target farms at higher risk of veterinary drug residue contamination for testing programs in combination with incentives, education and training programs to improve mammary health, milk hygiene and safety.

https://doi.org/10.1590/1678-992x-2016-0120 2080 downloads

Latin American consensus on guidelines for chronic migraine treatment
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Giacomozzi, Alex Rodrigo Espinoza

; Vindas, Alexander Parajeles

; Junior, Ariovaldo Alberto da Silva

; Bordini, Carlos Alberto

; Buonanotte, Carlos Federico

; Roesler, Célia Aparecida de Paula

; Brito, Cláudio Manoel

; Perez, Cristina

; Carvalho, Deusvenir de Souza

; Macedo, Djacir Dantas Pereira de

; Piovesan, Elcio Juliato

; Sarmento, Elder Machado

; Melhado, Eliana Meire

; Éckeli, Fabíola Dach

; Kowacs, Fernando

; Sobrino, Fidel

; Rabello, Getúlio Daré

; Rada, Grisel

; Souza, Jano Alves de

; Casanovas, Juana Rosa

; Durán, Juan Carlos

; Calia, Leandro Cotoni

; Medina, Luis Roberto Partida

; Queiroz, Luiz Paulo de

; Ciciarelli, Marcelo Cedrinho

; Valença, Marcelo Moraes

; Cusicanqui, Maria

; Jimenez, Maria Karina Velez

; Goycochea, Maria Tereza

; Peres, Mário Fernando Prieto

; Sandoval, Mario Victor Fuentealba

; Vincent, Maurice Borges

; Gomes, Michel Volcy

; Diez, Mónica

; Aranaga, Nayeska

; Barrientos, Nelson

; Kowacs, Pedro André

; Filho, Pedro Ferreira Moreira

Arquivos de Neuro-Psiquiatria

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jul 2013, Volume 71 N. 7 Pages 478 - 486

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A migrânea crônica é uma condição com prevalência significativa ao redor do mundo e alto impacto socioeconômico, sendo que seu manuseio tem desafiado os neurologistas. Os avanços na compreensão de seus mecanismos e das condições a ela associadas, bem como nas novas terapêuticas, têm sido rápidos e importantes, fato que motivou as Sociedades Latino-americana e Brasileira de Cefaleia a elaborarem o presente consenso. O tratamento da migrânea crônica deve ser sempre precedido por uma revisão cuidadosa do diagnóstico, pela detecção de possíveis fatores de piora e das condições associadas, pela estratificação de gravidade/impossibilidade de se tratar e pelo monitoramento com um diário da dor. Este consenso apresenta abordagens farmacológicas e não-farmacológicas para tratar a migrânea crônica.

Chronic migraine is a condition with significant prevalence all around the world and high socioeconomic impact, and its handling has been challenging neurologists. Developments for understanding its mechanisms and associated conditions, as well as that of new therapies, have been quick and important, a fact which has motivated the Latin American and Brazilian Headache Societies to prepare the present consensus. The treatment of chronic migraine should always be preceded by a careful diagnosis review; the detection of possible worsening factors and associated conditions; the stratification of seriousness/impossibility to treat; and monitoring establishment, with a pain diary. The present consensus deals with pharmacological and nonpharmacological forms of treatment to be used in chronic migraine.

https://doi.org/10.1590/0004-282X20130066 11632 downloads

Determinação da infecção por Entamoeba histolytica em residentes da área metropolitana de Belém, Pará, Brasil, utilizando ensaio imunoenzimático (ELISA) para detecção de antígenos
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Silva, Mônica Cristina de Moraes

; Monteiro, Christiane do Socorro Pereira

; Araújo, Bruna dos Anjos Veloso

; Silva, Jacilene Valdevina

; Póvoa, Marinete Marins

Cadernos de Saúde Pública

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jun 2005, Volume 21 N. 3 Pages 969 - 973

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O status epidemiológico da amebíase está sendo reavaliado desde que a Entamoeba histolytica (patogênica) foi considerada espécie distinta de Entamoeba dispar (não patogênica). Em nosso estudo, realizamos pesquisa de antígenos de E. histolytica em amostras fecais de pacientes residentes na cidade de Belém, Pará, Brasil, utilizando ensaio imunoenzimático (E. histolytica Test, TechLab Inc., Blacksburg, Estados Unidos) disponível comercialmente. Foram analisadas 845 amostras, com positividade em 248 (29,35%). A infecção por E. histolytica foi maior no grupo etário acima de 14 anos (30,36%) que no grupo de 0-14 anos (28,28%), porém sem significância estatística (p < 0,05). Do total de amostras analisadas, 334 foram também investigadas por métodos parasitológicos (direto, Hoffman e Faust et al.). Houve discordância nos resultados dos métodos ELISA e coproscópico em 83 amostras (24,85%), com maior número de positivos no teste imunoenzimático. Assim, nossos resultados sugerem que a amebíase intestinal é um importante problema de saúde pública na Região Metropolitana de Belém.

The epidemiological status of amebiasis has been reevaluated since Entamoeba histolytica (pathogenic) was considered a distinct species from Entamoeba dispar (non-pathogenic). We investigated E. histolytica antigens in stool samples from residents of Belém, Pará State, Brazil, with commercially available enzyme-linked immunosorbent assay (E. histolytica Test, TechLab Inc., Blacksburg, USA). A total of 845 samples were analyzed, of which 248 were positive (29.35%). E. histolytica infection was more frequent in the over-14-year age group (30.36%) than in the 0-14-year group (28.28%), but the difference was not statistically significant (p < 0.05). Of all the samples, 334 were also submitted to parasitological methods (direct, Hoffman, and Faust et al.). There were discordant results between ELISA and parasitological methods in 83 samples (24.85%), with more positive results using ELISA. Our results thus suggest that intestinal amebiasis is an important public health problem in Greater Metropolitan Belém.

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Silva, Mônica Cristina de Moraes

; Monteiro, Christiane do Socorro Pereira

; Araújo, Bruna dos Anjos Veloso

; Silva, Jacilene Valdevina

; Póvoa, Marinete Marins

Cadernos de Saúde Pública

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jun 2005, Volume 21 N. 3 Pages 969 - 973

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

5126 downloads

Diagnóstico de amebíase intestinal utilizando métodos coproscópicos e imunológicos em amostra da população da área metropolitana de Belém, Pará, Brasil
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Póvoa, Marinete Marins

; Arruda, José Eduardo Gomes

; Silva, Mônica Cristina de Moraes

; Bichara, Cléa Nazaré Carneiro

; Esteves, Paulo

; Gabbay, Yvone Benchimol

; Machado, Ricardo Luiz Dantas

Cadernos de Saúde Pública

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sep 2000, Volume 16 N. 3 Pages 843 - 846

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O artigo expõe a comparação de métodos de diagnóstico de Entamoeba histolytica em amostra da população de Belém do Pará. Foram analisadas amostras fecais de crianças e adultos (Grupo I), amostras fecais e soros de adultos (Grupo II) e material fecal de crianças (Grupo III). Nos grupos I e III foram empregados os métodos direto com lugol (MD), Faust e cols. (MFF) e ELISA (detecção de coproantígeno anti-GIAP de E. histolytica); no grupo II, MD, hematoxilina férrica (HF), MFF, ELISA e reação de imunofluorescência indireta (RIFI) para detecção de anticorpos IgG. A positividade encontrada foi de 10,50%, empregando (MD + MFF) e de 28,99% pelo ELISA. Não houve correlação entre positividade e grupo etário. No Grupo II (n = 87), a positividade encontrada foi de 4,59% pelos métodos coproscópicos (MD + MFF), 8,04% por HF, 4,59% pela RIFI e 21,83% pelo ELISA. O teste de ELISA foi o mais sensível para todos os grupos. Conclui-se que a RIFI ainda não é ferramenta útil para diagnóstico desta protozoose. O teste de ELISA, de fácil execução, foi feito em 1/3 do tempo usado para HF e RIFI, melhorando a qualidade do diagnóstico. Recomenda-se o ELISA como método de diagnóstico nos caso suspeitos de infecções com E. histolytica.

We compare diagnostic methods for Entamoeba histolytica in fecal samples from the city of Belém, Pará, Brazil. We analyze stool samples from children and adults (Group I); stool and serum samples from adults (Group II); and stool samples from children (Group III). In groups I and III, we used direct examination with lugol (DM), Faust et al (FM), and ELISA (detection of E. histolytica anti-GIAP coproantigen) and in group II, DM, iron hematoxylin staining (IHS), FM, ELISA, and the indirect immunofluorescence test (IFAT) for detection of IgG antibodies. Positivity was 10.50% by DM plus FM and 28.99% by ELISA. There was no correlation between positivity and age group. In Group II (n = 87), the positive rate was 4.59% by DM plus FM, 8.04% by IHS, 4.59% by IFAT, and 21.83% by ELISA. The ELISA test was the most sensitive for all groups. IFAT alone is still not a useful tool for diagnosis of E. histolytica infection. The ELISA test is simple, performed in one-third of cases used for IHS and IFAT, and greatly improves quality of diagnosis. We recommend this as the method of choice for diagnosis of suspected E. histolytica infection.

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Diagnóstico de amebíase intestinal utilizando métodos coproscópicos e imunológicos em amostra da população da área metropolitana de Belém, Pará, Brasil
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Póvoa, Marinete Marins

; Arruda, José Eduardo Gomes

; Silva, Mônica Cristina de Moraes

; Bichara, Cléa Nazaré Carneiro

; Esteves, Paulo

; Gabbay, Yvone Benchimol

; Machado, Ricardo Luiz Dantas

Cadernos de Saúde Pública

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sep 2000, Volume 16 N. 3 Pages 843 - 846

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

16871 downloads Cited 4 times in SciELO

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