Abstract To date, hydroxyurea is the only effective and safe drug that significantly reduces morbidity and mortality of individuals with Sickle cell disease. Twenty years of real-life experience has demonstrated that hydroxyurea reduces pain attacks, vaso-occlusive events, including acute chest syndrome, the number and duration of hospitalizations and the need for transfusion. The therapeutic success of hydroxyurea is directly linked to access to the drug, the dose used and adherence to treatment which, in part, is correlated to the availability of hydroxyurea. This consensus aims to reduce the number of mandatory exams needed to access the drug, prioritizing the requesting physician's report, without affecting patient safety. date disease reallife real life attacks vasoocclusive vaso occlusive events syndrome transfusion which part physicians physician s report safety

ABSTRACT Objective. Evaluate the implementation of the Ministry of Health’s “Action Plan: Border Vaccination Strategy – Agenda 2022” in the Brazil’s 33 twin cities and evaluate the increase in the country’s vaccination coverage (VC). Methodology. Pre-post community clinical trial. Implementation of the strategy was analyzed, and pre- and post-intervention VC were compared in two stages: P1 (pre-intervention) and P2 (post-intervention). Based on statistical analyses of P1 and P2 coverage, calculations were made of municipal averages, standard deviation, and difference in VC between the two periods. Results. Integration was observed between the primary health care (PHC), surveillance, immunization, and special indigenous health district (DSEI) teams, although there were difficulties, for example, in relation to migratory flows. While immigration flows present challenges in the areas of immunization, PHC, and DSEI, the difficulties are compounded by the polarization of these services, which hinders intersectoral integration. After carrying out the workshops, a total of 50 977 doses were administered in the general population in the 33 twin cities. There was an increase in vaccination coverage in children up to 1 year of age in the locations evaluated after the intervention, which may be relevant in terms of increasing VC in Brazil. Conclusion. There was an increase in vaccination coverage in children up to 1 year of age in the locations evaluated after the intervention, helping to increase VC in Brazil.

RESUMO Objetivo. Avaliar a implementação de estratégia do “Plano de Ação: Estratégia de Vacinação nas Fronteiras – Agenda 2022” do Ministério da Saúde nas 33 cidades gêmeas e avaliar o incremento das coberturas vacinais (CV) brasileiras. Métodos. Ensaio clínico comunitário, do tipo antes e depois. Analisou-se a realização da estratégia, bem como comparadas as CV pré e pós-intervenção em dois tempos: P1 (pré-intervenção) e P2 (pós-intervenção). Análises estatísticas sobre a CV, no P1 e P2, foram calculados os valores de média entres os municípios, desvio padrão e diferença entre as coberturas dos dois períodos. Resultados. Observou-se integração entre as equipes de Atenção Primária à Saúde (APS), Vigilância, Imunização e Distrito Sanitário Especial Indígena (DSEI), porém com dificuldades, como aquelas inerentes ao fluxo migratório. Ressalta-se que o fluxo imigratório é um dos desafios no contexto da imunização, APS e DSEI, entretanto, a esta dificuldade soma-se a polarização entre os serviços (imunização, APS e DISEI), ocasionando um desafio para a integração dos setores. Em relação à análise das CV, após a realização das oficinas, foram totalizadas 50 977 doses aplicadas na população geral nas 33 cidades-gêmeas do Brasil. Houve incremento das coberturas vacinais de crianças de até um ano de idade nos locais avaliados após a intervenção, o que pode ser importante para aumentar as CV no Brasil. Conclusão. Houve incremento das coberturas vacinais das crianças até um ano de idade nos locais avaliados após a intervenção e isso influenciou no aumento das CV no Brasil.

RESUMEN Objetivo. Evaluar la aplicación de la Estrategia de Vacunación en las Fronteras - Agenda 2022, que forma parte del Plan de Acción del Ministerio de Salud en las 33 ciudades hermanas y evaluar el aumento de las tasas de cobertura de vacunación en Brasil. Métodos. Ensayo clínico comunitario realizado antes y después de la intervención correspondiente. Se analizó la aplicación de la estrategia y se compararon las tasas de cobertura de vacunación antes y después de la intervención en dos periodos: P1 (pre-intervención) y P2 (post-intervención). En los análisis estadísticos de la tasa de cobertura de vacunación en P1 y P2 se calcularon los valores de media y desviación estándar de los municipios y la diferencia entre las tasas de cobertura de los dos periodos. Resultados. Se observó una integración entre los equipos de Atención Primaria de Salud, Vigilancia, Inmunización y el Distrito Especial de Salud Indígena (DISEI), pero con dificultades, como las inherentes al flujo migratorio. Cabe destacar que el flujo migratorio es uno de los desafíos en el contexto de la inmunización, la atención primaria de salud y el DISEI, dificultad que se ve agravada por la polarización entre los servicios (inmunización, atención primaria de salud y el DISEI), lo que supone un reto para la integración de los sectores. Por lo que respecta al análisis de las tasas de cobertura de vacunación llevado a cabo después de realizar los talleres, se administró un total de 50 977 dosis a la población general en las 33 ciudades hermanas de Brasil. Hubo un aumento de las tasas de cobertura de vacunación de menores de hasta un año de edad en los lugares evaluados después de la intervención, lo que puede ser importante para aumentar las tasas de cobertura de Brasil. Conclusión. Después de la intervención hubo un aumento de las tasas de cobertura de vacunación de menores de hasta un año de edad en los lugares evaluados, lo cual influyó en el incremento de las tasas de cobertura de Brasil.

Abstract Background: Spinal ventral root injuries generate significant motoneuron degeneration, which hinders full functional recovery. The poor prognosis of functional recovery can be attributed to the use or combination of different therapeutic approaches. Several molecules have been screened as potential treatments in combination with surgical reimplantation of the avulsed roots, the gold standard approach for such injuries. Among the studied molecules, human natural killer-1 (HNK-1) stands out as it is related to the stimulation of motor axon outgrowth. Therefore, we aimed to comparatively investigate the effects of local administration of an HNK-1 mimetic peptide (mp-HNK-1) and systemic treatment with ursolic acid (UA), another HNK-1 mimetic, after ventral root avulsion and reimplantation with heterologous fibrin biopolymer (HFB). Methods: Female mice of the isogenic strain C57BL/6JUnib were divided into five experimental groups: Avulsion, Reimplantation, mp-HNK-1 (in situ), and UA (systemic treatment). Mice were evaluated 2 and 12 weeks after surgery. Functional assessment was performed every four days using the Catwalk platform. Neuronal survival was analyzed by cytochemistry, and glial reactions and synaptic coverage were evaluated by immunofluorescence. Results: Treatment with UA elicited long-term neuroprotection, accompanied by a decrease in microglial reactions, and reactive astrogliosis. The neuroprotective effects of UA were preceded by increased glutamatergic and GABAergic inputs in the ventral spinal cord two weeks after injury. However, a single application of mp-HNK-1 had no significant effects. Functional analysis showed that UA treatment led to an improvement in motor and sensory recovery. Conclusion: Overall, the results indicate that UA is neuroprotective, acting on glial cells and synaptic maintenance, and the combination of these findings led to a better functional recovery. Background degeneration approaches roots killer1 killer 1 killer- HNK1 HNK (HNK-1 outgrowth Therefore HNK- mpHNK1 mpHNK mp (mp-HNK-1 UA, , (UA) HFB. HFB . (HFB) Methods C57BL6JUnib CBLJUnib C57BL 6JUnib C BL JUnib groups Avulsion Reimplantation mp-HNK- situ, situ situ) treatment. treatment) surgery platform cytochemistry immunofluorescence Results longterm long term neuroprotection astrogliosis injury However Conclusion Overall maintenance (HNK- (mp-HNK- (UA (HFB CBL mp-HNK (HNK (mp-HNK

RESUMO O câncer de pulmão (CP) é uma das neoplasias mais comuns e letais no Brasil, e apenas 15% dos pacientes são diagnosticados nos estágios iniciais. O tabagismo persiste como o responsável por mais de 85% de todos os casos. O rastreamento do CP (RCP) por meio da TC de baixa dosagem de radiação (TCBD) reduz a mortalidade do CP em 20%, e, quando combinado com a cessação do tabagismo, essa redução chega a 38%. Na última década, diversos países adotaram o RCP como recomendação de saúde populacional. No Brasil, embora ainda incipiente, a discussão sobre o tema é cada vez mais ampla e necessária. Com o intuito de aumentar o conhecimento e estimular o debate sobre o RCP, a Sociedade Brasileira de Cirurgia Torácica, a Sociedade Brasileira de Pneumologia e Tisiologia e o Colégio Brasileiro de Radiologia e Diagnóstico por Imagem constituíram um painel de especialistas para elaborar as recomendações para o RCP. As recomendações aqui apresentadas foram baseadas em revisão narrativa da literatura, com ênfase em grandes estudos populacionais, em revisões sistemáticas e em recomendações de diretrizes internacionais, sendo construídas após ampla discussão pelo grupo de especialistas. Os temas revisados foram os seguintes: porque rastrear, considerações gerais sobre tabagismo, epidemiologia do CP, critérios de elegibilidade, achados incidentais, lesões granulomatosas, modelos probabilísticos, requisitos mínimos da TCBD, aquisições volumétricas, riscos do rastreamento, estrutura mínima e papel da equipe multidisciplinar, conduta segundo o Lung CT Screening Reporting and Data System (Lung-RADS), custos vs. benefícios e perspectivas do rastreamento. (CP Brasil 15 iniciais 85 casos (RCP TCBD (TCBD 20 20% 38 38% década populacional incipiente necessária Torácica literatura populacionais internacionais seguintes rastrear elegibilidade incidentais granulomatosas probabilísticos volumétricas multidisciplinar LungRADS, LungRADS RADS , (Lung-RADS) vs 1 8 2 3 (Lung-RADS

ABSTRACT Although lung cancer (LC) is one of the most common and lethal tumors, only 15% of patients are diagnosed at an early stage. Smoking is still responsible for more than 85% of cases. Lung cancer screening (LCS) with low-dose CT (LDCT) reduces LC-related mortality by 20%, and that reduction reaches 38% when LCS by LDCT is combined with smoking cessation. In the last decade, a number of countries have adopted population-based LCS as a public health recommendation. Albeit still incipient, discussion on this topic in Brazil is becoming increasingly broad and necessary. With the aim of increasing knowledge and stimulating debate on LCS, the Brazilian Society of Thoracic Surgery, the Brazilian Thoracic Association, and the Brazilian College of Radiology and Diagnostic Imaging convened a panel of experts to prepare recommendations for LCS in Brazil. The recommendations presented here were based on a narrative review of the literature, with an emphasis on large population-based studies, systematic reviews, and the recommendations of international guidelines, and were developed after extensive discussion by the panel of experts. The following topics were reviewed: reasons for screening; general considerations about smoking; epidemiology of LC; eligibility criteria; incidental findings; granulomatous lesions; probabilistic models; minimum requirements for LDCT; volumetric acquisition; risks of screening; minimum structure and role of the multidisciplinary team; practice according to the Lung CT Screening Reporting and Data System; costs versus benefits of screening; and future perspectives for LCS. LC (LC tumors 15 stage 85 cases (LCS lowdose low dose (LDCT LCrelated related 20 20% 38 cessation decade populationbased population recommendation incipient necessary Surgery Association literature studies reviews guidelines reviewed criteria findings lesions models acquisition team System 1 8 2 3

BACKGROUND The Golden Syrian hamster (Mesocricetus auratus), Ferrets (Mustela putorius furo), and macaques have been described as useful laboratory animals naturally susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. OBJECTIVES To study the mechanism of lung injury, we describe the histopathological features of SARS-CoV-2 infection in Golden Syrian hamsters inoculated intranasally with the A.2 Brazilian strain. METHODS Hamsters were intranasally inoculated with the A.2 variant and euthanised at 3-, 5-, 10- and 15-days post-inoculation. The physical examination and body weight were recorded daily. Neutralising antibodies and viral RNA load of the respiratory tract were assessed during necropsies. FINDINGS The coronavirus disease 2019 (COVID-19) model presented body weight loss, high levels of respiratory viral RNA load, severe segmentary pneumonitis, and bronchial fistula besides lymphatic trapping and infiltration, like the human SARS-COV-2 pathogenesis. The presence of subepithelial lymphoeosinophilic infiltrate was highlighted in our results; it contributed to the detachment of SARS-CoV-2 nucleocapsid-positive epithelial cells resulting in the infectious cell plugs. MAIN CONCLUSIONS The SARS-CoV-2 caused segmentary pneumonia and vascular damage. In our comprehension, the infectious cell plugs, as being aspirated from the upper respiratory tract into the terminal bronchial lumen, work as a “Trojan horse”, thus contributing to the dissemination of the SARS-CoV-2 infection into specific regions of the deep lung parenchyma. Mesocricetus auratus, auratus , auratus) Mustela furo, furo furo) SARSCoV2 SARSCoV SARS CoV (SARS-CoV-2 injury SARS-CoV- A2 A A. strain 3, 3 3- 5, 5 5- 10 15days days 15 postinoculation. postinoculation post inoculation. inoculation post-inoculation daily necropsies 201 COVID19 COVID 19 (COVID-19 loss pneumonitis infiltration SARSCOV2 SARSCOV COV SARS-COV- pathogenesis results nucleocapsidpositive nucleocapsid positive plugs damage comprehension lumen Trojan horse, horse horse” parenchyma (SARS-CoV- SARS-CoV 1 20 COVID1 (COVID-1 SARS-COV (SARS-CoV (COVID- (COVID

ABSTRACT Background: The intensity of dengue virus (DV) replication and circulating non-structural protein 1 (NS1) levels may promote changes in the human immune response and favor severe forms of infection. We investigated the correlations between NS1 with CXCL-8, CXCL-10, IFN-γ, and IL-12p40 serum levels, and IFN-γ receptor α chain (CD119) expression, and CXCL10 production by peripheral blood mononuclear cells (PBMCs) stimulated with recombinant IFN-γ in DV-infected patients with different clinical forms. Methods: Dengue virus NS1, CXCL-8, CXCL-10, IFN-γ, and IL-12p40 serum levels were measured in 152 DV-infected patients with different clinical forms and 20 non-infected individuals (NI) using enzyme-linked immunosorbent assay (ELISA). In addition, we investigated the CXCL-10 production after in vitro IFN-γ stimulation of PBMCs from 48 DV-infected individuals (with different clinical forms of dengue fever) and 20 NI individuals using ELISA, and CD119 expression on CD14+ cells with flow cytometry. Results: Patients with dengue hemorrhagic fever (DHF) had significantly higher NS1, CXCL-8, and CXCL-10 serum levels than those with classic dengue fever (DF). The response of PBMCs to IFN-γ stimulation was lower in patients with DHF than in those with DF or dengue with complications (DWC), with lower CD119 expression and reduced CXCL-10 synthesis. In addition, these alterations are associated with high NS1 serum levels. Conclusions: Patients with DHF reported high NS1 levels, low CD119 expression, and low CXCL-10 synthesis in PBMCs, which may be associated with infection progression and severity. Background DV (DV nonstructural non structural NS (NS1 CXCL8, CXCL8 CXCL 8, 8 CXCL-8 CXCL10, 10, 10 IFNγ, IFNγ IFN γ, γ IL12p40 ILp IL 12p40 p IL-12p4 CD (CD119 CXCL1 (PBMCs DVinfected infected Methods 15 2 noninfected (NI enzymelinked enzyme linked ELISA. ELISA . (ELISA) addition CXCL-1 4 CD11 CD14 cytometry Results (DHF DF. (DF) DWC, DWC , (DWC) Conclusions severity (NS CXCL- IL12p4 12p4 IL-12p (CD11 (ELISA CD1 (DF (DWC IL12p 12p (CD1 (CD

Abstract This cross-sectional study investigated the association of SNPs rs670 (C>T) and rs693 (G>A) with parameters of lipid metabolism and endothelial activation in 167 subjects, including 86 COVID-19 outpatients and 81 healthy subjects (control group) matched for sex and age. Serum levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), non-high-density lipoprotein cholesterol (non-HDL-c), triglycerides (TG), apolipoproteins (Apo A-I and B), and vascular cell adhesion molecule-1 (VCAM-1) were determined. The SNPs were genotyped by quantitative polymerase chain reaction (qPCR). A p -value<0.05 was assumed. COVID-19 outpatients showed increases in TC (187.0±48.3 vs . 160.8±32.0 mg/dL), LDL-c (110.3±41.5 vs . 98.8±28.2 mg/dL), non-HDL-c (138.3±45.8 vs . 115.6±31.6 mg/dL), TG (139.7±80.3 vs . 84.9±35.1 mg/dL), and Apo A-I (149.5±40.0 vs . 133.3±20.9 mg/dL), along with a high frequency of hypercholesterolemia and hypertriglyceridemia. VCAM-1 levels were double those of the control (682.7±231.8 vs. 299.3±102.9 ng/mL) and a strong predictor of COVID-19 (AUC=0.946). Moreover, VCAM-1 correlated with TC (r = -0.223) and HDL-c (r = -0.225). The decrease in TG and VCAM-1 is predicted by the dominance of the T allele (rs670) and its codominance with the A (rs693) allele in COVID-19 outpatients. COVID-19 has been shown to be associated with dyslipidemias and endothelial activation, and apolipoprotein polymorphisms may influence this. crosssectional cross sectional rs rs67 C>T CT C (C>T rs69 G>A GA G (G>A 16 8 COVID19 COVID 19 COVID-1 group age TC, , (TC) highdensity density HDLc, HDLc HDL c (HDL-c) lowdensity low LDLc, LDLc LDL (LDL-c) nonhighdensity non nonHDLc, nonHDLc (non-HDL-c) TG, (TG) AI I B, B B) molecule1 molecule 1 molecule- VCAM1 VCAM (VCAM-1 determined qPCR. qPCR (qPCR) value<0.05 value005 value 0 05 -value<0.0 assumed 187.0±48.3 1870483 187 48 3 (187.0±48. 1608320 160 32 160.8±32. mg/dL, mgdL mg/dL mg dL mg/dL) 110.3±41.5 1103415 110 41 5 (110.3±41. 988282 98 28 2 98.8±28. 138.3±45.8 1383458 138 45 (138.3±45. 1156316 115 6 31 115.6±31. 139.7±80.3 1397803 139 7 80 (139.7±80. 849351 84 9 35 84.9±35. 149.5±40.0 1495400 149 40 (149.5±40. 1333209 133 20 133.3±20. hypertriglyceridemia VCAM- 682.7±231.8 68272318 682 231 (682.7±231. 29931029 299 102 299.3±102. ng/mL ngmL ng mL AUC=0.946. AUC0946 AUC AUC=0.946 946 (AUC=0.946) Moreover r 0.223 0223 223 -0.223 0.225. 0225 0.225 225 -0.225) (rs670 (rs693 this rs6 COVID1 COVID- (TC (HDL-c (LDL-c (non-HDL-c (TG (VCAM- (qPCR value<0.0 value00 -value<0. 187.0±48. 187048 18 4 (187.0±48 160832 160.8±32 110.3±41. 110341 11 (110.3±41 98828 98.8±28 138.3±45. 138345 13 (138.3±45 115631 115.6±31 139.7±80. 139780 (139.7±80 84935 84.9±35 149.5±40. 149540 14 (149.5±40 133320 133.3±20 682.7±231. 6827231 68 23 (682.7±231 2993102 29 10 299.3±102 AUC094 AUC=0.94 94 (AUC=0.946 0.22 022 22 -0.22 -0.225 (rs67 (rs69 (VCAM value<0. value0 -value<0 187.0±48 18704 (187.0±4 16083 160.8±3 110.3±41 11034 (110.3±4 9882 98.8±2 138.3±45 13834 (138.3±4 11563 115.6±3 139.7±80 13978 (139.7±8 8493 84.9±3 149.5±40 14954 (149.5±4 13332 133.3±2 682.7±231 682723 (682.7±23 299310 299.3±10 AUC09 AUC=0.9 (AUC=0.94 0.2 02 -0.2 (rs6 value<0 -value< 187.0±4 1870 (187.0± 1608 160.8± 110.3±4 1103 (110.3± 988 98.8± 138.3±4 1383 (138.3± 1156 115.6± 139.7±8 1397 (139.7± 849 84.9± 149.5±4 1495 (149.5± 1333 133.3± 682.7±23 68272 (682.7±2 29931 299.3±1 AUC0 AUC=0. (AUC=0.9 0. -0. (rs value< -value 187.0± (187.0 160.8 110.3± (110.3 98.8 138.3± (138.3 115.6 139.7± (139.7 84.9 149.5± (149.5 133.3 682.7±2 6827 (682.7± 2993 299.3± AUC=0 (AUC=0. -0 187.0 (187. 160. 110.3 (110. 98. 138.3 (138. 115. 139.7 (139. 84. 149.5 (149. 133. 682.7± (682.7 299.3 AUC= (AUC=0 - 187. (187 110. (110 138. (138 139. (139 149. (149 682.7 (682. 299. (AUC= (18 (11 (13 (14 682. (682 (AUC (1 (68 ( (6

Abstract Introduction Jet aircraft pilots are exposed to huge pressure variation during flight, which affect physiological functions as systems, such as the respiratory system. Objectives The objective of the present investigation was to evaluate inflammatory changes of paranasal sinuses of jet aircraft pilots before and after a jet aircraft training program, using multislice computed tomography (CT), in comparison with a group of nonairborne individuals with the same age, sex, and physical health conditions. A second objective of the present study was to assess the association between the ostiomeatal complex obstruction and its anatomical variations. Methods The study group consisted of 15 jet aircraft pilots participating in the training program. The control group consisted of 41 nonairborne young adults. The 15 fighter pilots were evaluated before initiating the training program and after their final approval for the presence of inflammatory paranasal sinus disease. The ostiomeatal complex anatomical variations and obstructions were analyzed in pilots after the training program. Results Jet aircraft pilots presented higher incidence of mucosal thickening in maxillary sinus and anterior ethmoid cells than controls. Prominent ethmoidal bulla showed significant association with obstruction of the osteomeatal complex. Conclusions Jet aircraft pilots present increased inflammatory disease when compared with nonairborne individuals. The presence of a prominent ethmoidal bulla is associated with ostiomeatal complex obstruction. flight systems system CT, CT , (CT) age sex conditions 1 4 adults controls (CT

Resumo A nutrição na criação de coelhos representa cerca de 70% de todos os custos; nesse sentido, a Cratylia argentea é uma excelente alternativa como alimento devido à sua composição bromatológica. Nosso objetivo foi avaliar o valor nutricional de dietas formuladas com folhas secas de C. argentea na alimentação de coelhos em crescimento. Foram utilizados 28 coelhos brancos da raça Nova Zelândia, com 55 dias de idade, de ambos os sexos. Dois tratamentos (n = 14, 7 machos e 7 fêmeas) foram avaliados em delineamento experimental de blocos casualizados em esquema fatorial 2 x 2. A dieta controle (CTRL) foi formulada para atender às exigências nutricionais de coelhos em crescimento, e a dieta teste (DRY) foi obtida substituindo 20% da dieta CTRL por folhas secas de C. argentea. O período experimental compreendeu 11 dias, sendo sete dias de adaptação e quatro dias de coleta total de fezes. O ganho médio diário, o consumo de matéria seca, a conversão alimentar e o peso corporal final não foram afetados pela substituição da dieta CTRL por folhas secas de C. argentea. O coeficiente de digestibilidade da matéria seca apresentou melhores resultados nos coelhos CTRL (Fêmea: 72,19%, Macho: 72,48%). Coelhos alimentados com a dieta CTRL apresentaram maior consumo de proteína bruta do que os alimentados com a dieta DRY, porém esses coelhos tiveram maior excreção e menor retenção de proteína bruta. Conclui-se que Cratylia argentea apresenta valores de 1.400,67 kcal de energia digestível por kg de matéria seca e 3,34% de proteína digestível por kg de matéria seca. 70 custos sentido bromatológica C crescimento Zelândia 5 idade sexos n 14 fêmeas (CTRL DRY (DRY 20 1 fezes diário Fêmea (Fêmea 7219 72 19 72,19% Macho 72,48%. 7248 72,48% . 48 72,48%) Concluise Conclui se 140067 400 67 1.400,6 334 3 34 3,34 721 72,19 724 72,48 4 14006 40 6 1.400, 33 3,3 72,1 72,4 1400 1.400 3, 72, 140 1.40 1.4 1.

Abstract Nutrition in rabbit farming accounts for about 70% of all costs; in this sense, C. argentea is an excellent alternative as a feed, due to its bromatological composition. Our goal is to evaluate the nutritional value of diets formulated with the dried leaves of C. argentea to feed growing rabbits. Twenty-eight white rabbits of the New Zealand breed, 55 days old, of both sexes, were used. Two treatments (n = 14, 7 males, and 7 females) were evaluated in a randomized block experimental design in a 2 x 2 factorial scheme. The control diet (CTRL) was formulated to meet the nutritional requirements of growing rabbits, and the test diet (DRY) was obtained by replacing 20% of the CTRL diet with dried leaves of C. argentea. The experimental period was comprised of 11 days, with seven days of adaptation and four days of total feces collection. The average daily gain, dry matter intake, feed conversion, and final body weight were not affected by replacing the CTRL diet with the dried leaves of C. argentea. The coefficient of dry matter digestibility showed better results in CTRL rabbits (female: 72.19%, male: 72.48%). Rabbits fed with the CTRL diet exhibited a higher consumption of crude protein compared to those fed with the DRY diet, however, these rabbits showed higher elimination and lower retention of crude protein. It is concluded that C. argentea presents values of 1,400.67 kcal per kg of dry matter, digestible energy, and 3.34% digestible protein per kg of dry matter. 70 costs sense C composition Twentyeight Twenty eight breed 5 old sexes used n 14 males females scheme (CTRL (DRY 20 1 collection gain intake conversion female (female 7219 72 19 72.19% male 72.48%. 7248 72.48% . 48 72.48%) however 140067 400 67 1,400.6 energy 334 3 34 3.34 721 72.19 724 72.48 4 14006 40 6 1,400. 33 3.3 72.1 72.4 1400 1,400 3. 72. 140 1,40 1,4 1,

ABSTRACT Objective To evaluate deaths, hospitalizations, and persistence of symptoms in patients with COVID-19 after infection in an outpatient setting during the first COVID-19 wave in Brazil. Methods This prospective cohort was between April 2020 and February 2021. Hospitalized or non-hospitalized COVID-19 patients until five days after symptom onset were included. The outcomes measured were incidence of death, hospitalization, and persistence of more than two symptoms 60 days after discharge. Results Out of 1,198 patients enrolled in the study, 66.7% were hospitalized. A total of 289 patients died (1 [0.3%] non-hospitalized and 288 [36%] hospitalized). At 60 days, patients non-hospitalized during admission had more persistent symptoms (16.2%) compared to hospitalized (37.1%). The COVID-19 severity variables associated with the persistence of two or more symptoms were increased age (OR= 1.03; p=0.015), respiratory rate at hospital admission (OR= 1.11; p=0.005), length of hospital stay of more than 60 days (OR= 12.24; p=0.026), and need for intensive care unit admission (OR= 2.04; p=0.038). Conclusion COVID-19 survivors who were older, tachypneic at admission, had a hospital length of stay >60 days, and were admitted to the intensive care unit had more persistent symptoms than patients who did not require hospitalization in the early COVID-19 waves. ClinicalTrials.gov Identifier: NCT04479488. deaths hospitalizations COVID19 COVID 19 COVID-1 Brazil 202 2021 nonhospitalized non included death 6 discharge 1198 1 198 1,19 study 667 66 7 66.7 28 ( 0.3% 03 0 3 [0.3% 36% 36 [36% . hospitalized) 16.2% 162 16 2 (16.2% 37.1%. 371 37.1% 37 (37.1%) OR= OR (OR 1.03 103 p=0.015, p0015 p p=0.015 , 015 p=0.015) 1.11 111 11 p=0.005, p0005 p=0.005 005 p=0.005) 12.24 1224 12 24 p=0.026, p0026 p=0.026 026 p=0.026) 2.04 204 04 p=0.038. p0038 p=0.038 038 p=0.038) older >6 waves ClinicalTrialsgov ClinicalTrials gov Identifier NCT04479488 NCT COVID1 COVID- 20 119 1,1 66. 0.3 [0.3 [36 16.2 (16.2 37.1 (37.1% 1.0 10 p001 p=0.01 01 1.1 p000 p=0.00 00 12.2 122 p002 p=0.02 02 2.0 p003 p=0.03 > NCT0447948 1, 0. [0. [3 16. (16. 37. (37.1 1. p00 p=0.0 12. 2. NCT044794 [0 [ (16 (37. p0 p=0. NCT04479 (37 p=0 NCT0447 (3 p= NCT044 NCT04 NCT0

Abstract We investigated the vasodilatory effects of Hymenaea rubriflora Ducke stem bark extract (HR- HAc). Vascular reactivity of the aortic rings of Wistar rats was tested by in vitro cumulative doses (0.1 - 729 μg/mL). Rats (n=5) were treated with 25 (G25), 50 (G50) and 100 (G100) mg/ kg of HR-HAc or saline (control group - CG) for four weeks. An in vitro assay resulted in dose-dependent relaxation of the aortic rings with functional endothelium, which was inhibited in the presence of L-NAME. Rings of the treated animals increased acetylcholine relaxing potency at all doses, with a greater effect on G50 (pD2 = 7.8±0.1, Emax = 95.6±1.1) and a decreased contractile potency to phenylephrine in G25 (pD2 = 6.9±0.06, Emax = 61.5±6.0%) and G50 (pD2= 6.6±0.06, Emax = 71.0±8.5%) when compared to the CG in the presence and absence of endothelium (pD2= 6.4± 0.1, 6.4±0.1 and 6.9±0.1, respectively). Cumulative doses of nitroprusside resulted in increased relaxing potency in all treated groups and maintained Emax at 100%. It is concluded that HR-HAc has vasorelaxant capacity and inhibitory vascular contraction activity applied either directly to aortic rings or after treatment with in vivo supplementation, which places this extract as a potential nutraceutical or pharmacological agent for treating diseases associated with vascular dysfunction. HR (HR HAc. HAc . HAc) 0.1 01 0 1 (0. 72 μg/mL. μgmL μg/mL μg mL μg/mL) n=5 n5 n 5 (n=5 2 G25, G , (G25) (G50 10 G100 (G100 mg HRHAc control weeks dosedependent dose dependent LNAME. LNAME L NAME. NAME L-NAME G5 pD2 pD (pD 7801 7 8 7.8±0.1 95.6±1.1 95611 95 6 G2 69006 9 06 6.9±0.06 61.5±6.0% 61560 61 pD2= 66006 6.6±0.06 71.0±8.5% 71085 71 64 4 6.4 6401 6.4±0. 6901 6.9±0.1 respectively. respectively respectively) 100% supplementation dysfunction 0. (0 n= (n= (G25 (G5 G10 (G10 780 7.8±0. 95.6±1. 9561 6900 6.9±0.0 61.5±6.0 6156 6600 6.6±0.0 71.0±8.5 7108 6. 640 6.4±0 690 6.9±0. ( (n (G2 (G G1 (G1 78 7.8±0 95.6±1 956 61.5±6. 615 660 6.6±0. 71.0±8. 710 69 6.9±0 7.8± 95.6± 61.5±6 66 6.6±0 71.0±8 6.9± 7.8 95.6 61.5± 6.6± 71.0± 6.9 7. 95. 61.5 6.6 71.0 61. 71.

RESUMO Este estudo partiu da premissa de que é possível estabelecer um diálogo entre biblioteca, ensino e aprendizagem, considerando que são partes complementares. Objetivo: O objetivo deste artigo foi mapear trabalhos científicos que demonstraram como se deu a integração das bibliotecas brasileiras no uso das metodologias ativas no processo de ensino e aprendizagem. Método: O método utilizado foi a pesquisa bibliográfica com abordagem qualitativa, à luz da Análise do Conteúdo. Resultado: Os resultados demonstraram que aliar a biblioteca às metodologias de aprendizagem ativa contribuem para uma formação integrada, pautada pela autonomia e protagonismo do estudante na construção do seu conhecimento e sugerem que fazer a interlocução da biblioteca com a prática educativa, requer sobretudo, um diálogo entre a biblioteca, o corpo docente e a equipe pedagógica. Conclusões: Espera-se contribuir para a construção de novos sentidos para a biblioteca escolar, que explorem todo seu potencial formativo. Conclui-se que a biblioteca escolar se relaciona com o processo de ensino e aprendizagem de forma tensa, mas que existe um diálogo sendo construído à passos lentos, rumo ao alinhamento de sua função às propostas pedagógicas que objetivam uma formação mais integrativa do estudante. complementares Objetivo Método qualitativa Conteúdo Resultado integrada educativa sobretudo pedagógica Conclusões Esperase Espera formativo Concluise Conclui tensa lentos

ABSTRACT This study was based on the premise that it is possible to establish a dialogue between library, teaching and learning, considering that they are complementary parts. Objetivo: The objective of this article was to map scientific works that demonstrated how Brazilian libraries were integrated into the use of active methodologies in the teaching and learning process. Methods: The method used was bibliographical research with a qualitative approach, in the light of Content Analysis. Results: The results demonstrated that combining the library with active learning methodologies contributes to integrated training, guided by the student's autonomy and protagonism in the construction of their knowledge and suggest that interconnecting the library with educational practice requires, above all, a dialogue between the library, teaching staff and pedagogical team. Conclusions: It is expected to contribute to the construction of new meanings for the school library, which explore its full educational potential. It is concluded that the school library relates to the teaching and learning process in a tense way, but that there is a dialogue being built slowly, towards aligning its function with pedagogical proposals that aim for a more integrative training of the student. parts Objetivo Methods approach Analysis Results students student s requires all team Conclusions potential way slowly

ABSTRACT Background: Vectorial transmission through hematophagous triatomine insects remains the primary mode of Chagas Disease contagion. These insects have become increasingly common in urban environments. Therefore, this study aimed to report an encounter of triatomines with trypanosomatid infection in a vertical residential condominium in Rio Branco, the capital of the state of Acre, in the western Brazilian Amazon. Methods: Triatomines were collected from residents and sent to the municipality's Entomological Surveillance sector. Trypanosomatid positivity was evaluated using optical microscopy, followed by species and genotype identification using molecular biology techniques. Results: Twenty-five adult triatomine specimens were collected from two of three condominium buildings invading apartments from the 2nd to 13th floors. Six specimens were identified as Rhodnius sp. and 19 as R. montenegrensis. Among these, molecular tests were conducted on seven specimens, with five testing positive for Trypanosoma cruzi, all belonging to genotype TcI. Conclusions: These findings underscore the need for further studies to better understand the invasive capacity of these insects in these environments and the mechanisms involved in this process. Background contagion Therefore Branco Acre Amazon Methods municipalitys municipality s sector microscopy techniques Results Twentyfive Twenty nd th floors sp 1 R montenegrensis cruzi TcI Conclusions process

Resumo A madeira é uma matéria-prima que desempenha um papel fundamental na construção civil ao longo do tempo. Identificar quais eram as espécies utilizadas em edificações consideradas patrimônio histórico brasileiro permite contribuir com a conservação, bem como comparar o uso de madeiras em diferentes épocas. Sob essa perspectiva, a presente pesquisa teve como objetivo identificar e caracterizar a madeira utilizada na construção dos edifícios históricos “Casa de Dona Bém Bém” e “Gráfica Pepe”, localizados em Cuiabá, Mato Grosso, com o intuito de contribuir para a preservação do patrimônio histórico brasileiro. Para tal, foram avaliadas as propriedades organolépticas e anatômicas, por análises macro e microscópicas, realizando um estudo quali-quantitativo. Foram identificados oito gêneros distintos, sendo eles: Vochysia sp., Cedrela sp., Handroanthus sp., Physocalymma sp., Myracroduon sp., Dipteryx sp., Calophyllum sp. e Cordia sp. Além da identificação, buscou-se apontar o domínio fitogeográfico de tais gêneros para entender o contexto histórico da utilização dessas madeiras. Sugere-se que a substituição das peças deterioradas seja por outras das mesmas espécies madeireiras aqui identificadas, a fim de preservar a identidade dessas edificações históricas. matériaprima matéria prima tempo conservação épocas perspectiva Casa Gráfica Pepe, Pepe , Pepe” Cuiabá Grosso tal anatômicas microscópicas qualiquantitativo. qualiquantitativo quali quantitativo. quantitativo quali-quantitativo distintos eles sp identificação buscouse buscou se Sugerese Sugere identificadas históricas

Abstract Wood is a raw material that has played a key role in civil construction over time. Identifying which species were used in buildings considered Brazilian historical heritage sites will contribute to conservation, as well as the comparison of the use of wood at different times. From this perspective, the present study aimed to identify and characterize the wood used in the construction of the “Casa de Dona Bém Bém” and “Gráfica Pepe” historic buildings located in Cuiabá, Mato Grosso, aiming to contribute to the preservation of Brazilian historical heritage. To do so, the organoleptic and anatomical properties were evaluated by macro- and microscopic analysis, and a quali-quantitative study was conducted. Eight distinct genera were identified, namely, Vochysia sp., Cedrela sp., Handroanthus sp., Physocalymma sp., Myracroduon sp., Dipteryx sp., Calophyllum sp. and Cordia sp. In addition to identification, we sought to identify the phytogeographic domain of such genera to understand the historical context of the use of these woods. It is suggested that any replacement of deteriorated parts be with others of the same wood species identified herein to preserve the identity of these historic buildings. time conservation times perspective Casa Gráfica Pepe Cuiabá Grosso so macro analysis qualiquantitative quali quantitative conducted namely sp identification woods

RESUMO Apresentam-se, neste texto, dados resultantes de estudo documental e bibliográfico que analisa o oferecimento de disciplinas específicas relacionadas à literatura literária na área de conhecimento da educação de jovens e adultos (EJA), nas matrizes curriculares dos cursos de três universidades públicas localizadas nos estados de Mato Grosso do Sul, Paraná e São Paulo. Os cursos não apresentam nos Projetos Político-Pedagógicos disciplinas específicas cujos títulos remetam direta ou nominalmente à EJA ou ao seu público-alvo. A ampla maioria não aborda nas disciplinas relacionadas à literatura literária a questão dos jovens e adultos. Isso implica uma lacuna em termos de literatura literária na formação dos pedagogos. A pedagogia, no que tange à docência na educação escolar, habilita legalmente para a atuação também na EJA, que tem especificidades curriculares mesmo em termos de conteúdos e metodologias, dadas as faixas etárias abrangidas por essa modalidade. Apresentamse, Apresentamse Apresentam se, se Apresentam-se texto , (EJA) Sul Paulo PolíticoPedagógicos Político Pedagógicos públicoalvo. públicoalvo público alvo. alvo público-alvo pedagogos pedagogia escolar metodologias modalidade (EJA

ABSTRACT This paper presents data from a documental and bibliographic study that analyzes the offering of specific courses related to literary literature in the field of youth and adult education (EJA) in the curricula of three public universities located in the states of Mato Grosso do Sul, Paraná and São Paulo. The courses do not present, in their Political-Pedagogical Projects, specific subjects whose titles refer directly or nominally to EJA or its target audience. The vast majority do not address the issue of young people and adults in the disciplines related to literary literature. This implies a gap in terms of literary literature in the training of pedagogues. Pedagogy, as far as teaching in school education is concerned, legally qualifies students to act also in EJA, which has curricular specificities, including in terms of contents and methodologies, due to the age groups covered by this modality. (EJA Sul Paulo present PoliticalPedagogical Political Pedagogical Projects audience pedagogues Pedagogy concerned specificities methodologies modality

RESUMEN Este trabajo presenta datos de un estudio documental y bibliográfico que analiza la oferta de disciplinas específicas relacionadas con la literatura literaria en el área de Educación de Jóvenes y Adultos (EJA) en los currículos de los cursos de tres universidades públicas ubicadas en los estados de Mato Grosso do Sul, Paraná y São Paulo. Los cursos no presentan en sus Proyectos Político-Pedagógicos temas específicos cuyos títulos se refieran directa o nominalmente a la EJA o a su público objetivo. La gran mayoría no aborda en las disciplinas relacionadas con la literatura el tema de los jóvenes y adultos. Esto implica un vacío en cuanto a la literatura literaria en la formación de pedagogos. La pedagogía, en lo que se refiere a la enseñanza en la educación escolar, habilita legalmente para actuar también en la EJA, que tiene especificidades curriculares, incluso en términos de contenidos y metodologías, debido a los grupos de edad abarcados por esta modalidad. (EJA Sul Paulo PolíticoPedagógicos Político Pedagógicos objetivo adultos pedagogos pedagogía escolar curriculares metodologías modalidad