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1.
Computer-Aided Drug Design Studies in Association with in vitro Antileishmanial Tests for New Chalcones ComputerAided Computer Aided
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Silva, Gleice R. da
; Santos, Francisnaira S.
; Leite, Fernando F.
; Acevedo, Chonny A. H.
; Sousa, Natália F. de
; Grimaldi, Gabriela B.
; Soares, Milena B. P.
; Guimarães, Elisalva T.
; Scotti, Marcus T.
; Rodrigues, Luis Cezar
; Mendonça Júnior, Francisco J. B.
; Campana, Eloísa H.
; Barbosa Filho, José M.
; Guimarães, Hemerson I. F.
; Guerra, Felipe Q. S.
.
In silico and in vitro tests can reveal promising anti-leishmania activity for natural products and their derivatives. The aim of this study was to investigate in silico the pharmacological activities of potential new chalcones and their leishmanicidal potential in vitro. The in silico study was carried out using the PASS, MolPredictX and Molegro Virtual Docker 6.0 programs. Antiparasitic activity was assessed in axenic promastigote and amastigote forms of Leishmania braziliensis. The cytotoxicity tests used the J77G8 cell line. The chalcones exhibited 50% cytotoxic concentration values (CC50) values > 50 μM. Chalcone 4 (named FERAI) presented the best activity with concentration for 50% of promastigotes and intracellular parasites forms (EC50) of 9.75 ± 1.7 and 10.13 ± 1.7 μM for promastigote and amastigote, respectively. Reactive oxygen species (ROS) testing presented increased ROS levels in the parasite at the FERAI concentrations of 10 μM (56.33%), 20 μM (61.76%) and 30 μM (67.13%). Molecular docking revealed interactions (binding energy) between FERAI and the enzymes UDP-glycosyl pyrophosphorylase (-56.8384), dihydroorotate-dehydrogenase (-132.276) and trypanothione-reductase (-151.281). Our results demonstrated the anti-leishmanial activity of chalcones, especially FERAI, with a noted raising of ROS levels in the parasite. Molecular docking revealed dihydroorotate dehydrogenase and trypanothione reductase as potential pharmacological targets for FERAI. antileishmania anti leishmania derivatives PASS 60 6 0 6. programs braziliensis JG J G J77G line CC50 CC (CC50 5 named EC50 EC (EC50 975 9 75 9.7 17 1 7 1. 1013 13 10.1 respectively (ROS 56.33%, 5633 56.33% , 56 33 (56.33%) 2 61.76% 6176 61 76 (61.76% 3 67.13%. 6713 67.13% . 67 (67.13%) binding energy UDPglycosyl UDP glycosyl 56.8384, 568384 56.8384 8384 (-56.8384) dihydroorotatedehydrogenase 132.276 132276 132 276 (-132.276 trypanothionereductase 151.281. 151281 151.281 151 281 (-151.281) antileishmanial leishmanial CC5 (CC5 EC5 (EC5 97 9. 101 10. 563 56.33 (56.33% 61.76 617 (61.76 671 67.13 (67.13% 56838 56.838 838 (-56.8384 132.27 13227 27 (-132.27 15128 151.28 15 28 (-151.281 (CC (EC 56.3 (56.33 61.7 (61.7 67.1 (67.13 5683 56.83 83 (-56.838 132.2 1322 (-132.2 1512 151.2 (-151.28 56. (56.3 61. (61. 67. (67.1 568 56.8 8 (-56.83 132. (-132. 151. (-151.2 (56. (61 (67. (-56.8 (-132 (-151. (56 (6 (67 (-56. (-13 (-151 (5 ( (-56 (-1 (-15 (-5 (-
2.
Improving understanding of the plastochron of determinate and indeterminate soybean cultivars
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Porta, Felipe S. D.
; Streck, Nereu A.
; Alberto, Cleber M.
; Silva, Michel R. da
; Tura, Enrico F.
; Uliana, Gilnei F.
; Tagliapietra, Eduardo L.
; Alves, Alexandre F.
; Winck, José E. M.
; Soares, Maurício F.
; Zanon, Alencar J.
.
Revista Brasileira de Engenharia Agrícola e Ambiental
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RESUMO O objetivo deste estudo foi identificar as fases de plastocrono em cultivares de soja com hábito de crescimento determinado e indeterminado. Os experimentos foram conduzidos no período de 2010/2011 a 2022/2023 em quatro locais do estado do Rio Grande do Sul, Brasil, com 25 cultivares de soja com hábito de crescimento determinado (6) e indeterminado (19). Este estudo buscou dividir o plastocrono em fases durante o período de emissão de nós da soja. Em cultivares irrigadas de hábito de crescimento determinado, o plastocrono apresenta duas fases, a fase inicial de 59,7 °C dia nó-1 e a fase final de 65,7 °C dia nó-1. Em ambientes não irrigados, não há diferença entre as fases inicial e final do plastocrono. As cultivares de hábito de crescimento indeterminado com irrigação apresentam um plastocrono médio inicial e final de 64,1 °C dia nó-1, ao contrário da fase intermediária onde o plastocrono é de 58,5 °C dia nó-1. Em ambientes de sequeiro, as fases inicial e intermédia têm um valor médio de 60,6 °C dia nó-1, ao contrário da fase final com um plastocrono de 79,4 °C dia nó-1. A divisão do plastocrono em fases durante o período de emissão de nós na cultura da soja, proposta neste estudo, permite-nos melhorar o entendimento do desenvolvimento vegetativo de cultivares de soja de hábito de crescimento determinado e indeterminado e estabelecer bases ecofisiológicas para a construção de uma função cronológica. 20102011 2010 2011 2010/201 20222023 2022 2023 2022/202 Sul Brasil 2 6 (6 19. 19 . (19) 597 59 7 59, C nó1 nó 1 nó- 657 65 65, nó1. 1. irrigados 641 64 64, nó1, 1, 585 58 5 58, sequeiro 606 60 60, 794 79 4 79, permitenos permite nos cronológica 2010201 201 2010/20 2022202 202 2022/20 ( (19 201020 20 2010/2 202220 2022/2 (1 20102 2010/ 20222 2022/
ABSTRACT The aim of this study was to identify plastochron phases in soybean cultivars with determinate and indeterminate growth habits. Experiments were conducted from 2010/2011 to 2022/2023 at four locations in Rio Grande do Sul state (RS), Brazil, using 25 soybean cultivars with determinate (6) and indeterminate (19) growth habits. This study divided the plastochron into phases during the soybean node emission stage. In irrigated cultivars with a determinate growth habit, the plastochron has an early and late phase of 59.7 and 65.7 °C day node-1, respectively. In rainfed (non-irrigated) environments, there is no difference between the early and late plastochron phases. The early and late plastochrons of irrigated cultivars with an indeterminate growth habit are 64.1 °C day node-1, while the intermediate phase is 58.5 °C day node-1. In rainfed environments, the average value of the early and intermediate phases is 60.6 °C day node-1, with a late plastochron of 79.4 °C day node-1. Dividing the plastochron into phases during node emission, as proposed in this study, improves understanding of vegetative development in soybean cultivars with determinate and indeterminate growth habits and establishes ecophysiological foundations for chronological function construction. 20102011 2010 2011 2010/201 20222023 2022 2023 2022/202 RS, RS , (RS) Brazil 2 6 (6 19 (19 stage 597 59 7 59. 657 65 65. C node1, node1 1, 1 node-1 respectively nonirrigated non (non-irrigated environments 641 64 64. 585 58 5 58. node1. 1. 606 60 60. 794 79 4 79. construction 2010201 201 2010/20 2022202 202 2022/20 (RS ( (1 node- 201020 20 2010/2 202220 2022/2 20102 2010/ 20222 2022/
3.
Anatomical and carcass traits, partition of fat deposits, and meat quality in culled adult goats finished with high-fat diet
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Oliveira, Felipe B. B.
; Fernandes, César C. L.
; Alves, Juliana P. M.
; Marques, Iolly T. O.
; Silva, Caroline P.
; Lima, Francisco W. R.
; Carneiro, Hilton A. V.
; Filho, Nauara M. Lage
; Rêgo, Aníbal C.
; Rondina, Davide
.
Abstract The objective of this study was to examine the impact of a high-fat finishing diet on the in vivo performance, anatomical and carcass characteristics, and meat quality of adult culled goats. Over a period of 28 days leading up to slaughter, eighteen adult culled goats were subjected to a finishing diet provided in quantities sufficient to meet 3.0 times the nutritional requirements of adult non-dairy maintenance goats. The female goats were divided into two groups: one fed with the baseline diet without fat supplementation (n = 9) and the other fed a diet concentrated supplemented with whole full-fat linseed (n = 9). The diets varied in fat content (2.8% vs. 8.4% DM). The high-fat group exhibited a higher dry matter intake compared to the control group (+37%), along with elevated plasma cholesterol and triglyceride levels. The control group experienced a significant decrease in daily weight gain between the second and final week of the finishing period (-70%), in contrast to the increase observed in the high-fat group (+59%). At slaughter, no differences were noted between the groups in anatomical and carcass characteristics. Additionally, no differences were found between the diets regarding proximate composition, pH, yellow color component, and lightness of loin. The high-fat group exhibited a higher redness of loin. The inclusion of high-fat levels in finishing diets for culled adult goats allows for increased feed intake and performance but does not appear to affect carcass or meat quality.
4.
In silico Analysis of Natural Products from Brazilian Biodiversity in COVID-19 Treatment: NuBBE Database against SARS-CoV-2 Papain-Like Protease COVID19 COVID 19 COVID-1 Treatment SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- PapainLike Papain Like COVID1 1 COVID- SARS-CoV
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Cheohen, Caio Felipe A. R.
; Esteves, Maria Eduarda A.
; Gomes, Vinnícius M. S.
; Allonso, Diego
; Silva, Manuela L. da
.
Coronavirus disease (COVID-19) pandemic has infected 630 million people and led to 6.59 million deaths worldwide since its outbreak. To control COVID-19 advance, finding new molecules with potential to inhibit viral spread is pivotal. Papain-like protease (PLpro) is one of the most interesting targets of pharmacological inhibition since it is implicated not only in viral replication but also in modulation of host immune response. Natural products are of great interest to the pharmaceutical industry due to their diverse structure and biological activities. The NuBBEDB (Nuclei of Bioassays, Biosynthesis and Ecophysiology of Natural Products Database) is an excellent source of natural products (NPs) from the Brazilian flora. In this study, we performed virtual screening, molecular dynamics, and binding energy analyses of the NuBBEDB library to target severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) papain-like protease (PLpro), the virus’s protease essential for replication. Among the top-ranked molecules, the indole alkaloids raputindoles A, C, and D emerged as potential SARS-CoV-2 inhibitors, showing significant interactions with the pivotal Beta-Loop-2 (BL2) region, including the catalytic Y268 residue. Notably, raputindole D displayed enhanced stabilization of the BL2 region, while raputindole C exhibited superior overall stability. These in silico findings suggest that raputindoles, especially D, might offer therapeutic value against COVID-19, laying the groundwork for further experimental evaluations. COVID19 COVID 19 (COVID-19 63 659 6 59 6.5 outbreak COVID-1 advance Papainlike Papain like PLpro (PLpro response activities Nuclei Bioassays Database NPs (NPs flora study screening dynamics SARSCoV2 SARSCoV SARS CoV (SARS-CoV-2 papainlike papain PLpro, , viruss virus s topranked top ranked A SARS-CoV- inhibitors BetaLoop2 BetaLoop Beta Loop Beta-Loop- BL (BL2 region Y Y26 residue Notably stability COVID19, 19, evaluations COVID1 1 (COVID-1 65 5 6. COVID- (SARS-CoV- SARS-CoV Beta-Loop (BL Y2 (COVID- (SARS-CoV (COVID
5.
Annotation of Alkaloids of Fusaea longifolia and Evaluation of Anti-Plasmodium Activity in vitro and in silico AntiPlasmodium Anti Plasmodium
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Adrião, Asenate A. X.
; Silva, Carlos V. A. da
; Araujo, Morgana S.
; Paz, Weider H. P.
; Medeiros, Lívia S. de
; Silva, Felipe M. A. da
; Bezerra, Daniel P
; Salimo, Zeca M.
; Monteiro, Wuelton M.
; Melo, Gisely C. de
; Costa, Emmanoel V.
; Tavares, Josean F.
; Koolen, Hector H. F.
.
Isoquinoline alkaloids, especially from the Annonaceae family, have shown biological potential against parasites. Thus, this study aimed to evaluate the potential of the alkaloid fractions of the plant Fusaea longifolia against Plasmodium falciparum and annotate the compounds present in these samples. The tentative characterization of the alkaloids from the leaves and branches of F. longifolia was performed using liquid chromatography coupled to mass spectrometry (LC-MS/MS) and molecular networks. Through manual interpretation of the MS/MS spectra, 18 alkaloids were dereplicated from F. longifolia, 17 of which were reported for the first time in this species. An unpublished putative glycosylated alkaloid was annotated by interpreting the fragmentation data profile. Regarding biological activity, the fractions studied showed high activity against P. falciparum with half-maximal inhibitory concentration (IC50) of 2.42 and 1.60 μg mL-1 for branches and from the leaves, respectively, both similar to the reference standard quinine (IC50 of 1.24 μg mL-1). The structures of the 17 alkaloids were subjected to in silico analysis using molecular docking against four enzymes related to anti-Plasmodium activity (wild type (dm-PfDHFR) and mutant type (qm-PfDHFR), dihydroorotate dehydrogenase (PfDHODH) and purine nucleoside phosphorylase (PfPNP)). Molecular docking revealed strong interactions, especially between oxoxylopine 17 and hydroxycassythicine N-oxide 10, which may be potential new sources against P. falciparum. family parasites Thus samples F LCMS/MS LCMSMS LC MS (LC-MS/MS networks MSMS spectra 1 species profile P halfmaximal half maximal IC50 IC 242 2 42 2.4 160 60 1.6 mL1 mL mL- respectively (IC5 124 24 1.2 mL1. . mL-1) antiPlasmodium anti wild dmPfDHFR dm PfDHFR (dm-PfDHFR qmPfDHFR, qmPfDHFR qm , (qm-PfDHFR) PfDHODH (PfDHODH PfPNP. PfPNP (PfPNP)) interactions Noxide N oxide 10 LCMS IC5 4 2. 16 6 1. (IC 12 (qm-PfDHFR (PfPNP) (PfPNP
6.
Mating sanctuary for whitespotted eagle rays (Aetobatus narinari) in Brazilian southeastern waters Aetobatus narinari
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Karlovic, Thamíris C.
; Longo, Gabriela R.
; Lopes, Arielly R.
; Amaral, Iara L.
; Yoshino, Yara A.
; Almeida, Amanda A. V.
; Andrade, Lucas S.
; Bedin, Felipe G.
; Abreu, Marcos T. de
; Chioatto, Francesco S. M.
; Kian, Carolina Y.
; Candido, Lucas C.
; Silva, Joyce M.
; Moreira, Priscila S.
; Dias, June F.
.
Abstract This article provides the first records of courtship behavior and a mating event of free-ranging whitespotted eagle rays ( Aetobatus narinari ) in Brazilian waters. These observations shed light on utilizing the marine no-take zone surrounding the Anchieta Island State Park as a mating site in the South Atlantic region. Sightings of the species in shallower areas notably increased throughout summer months (from January to March, 2023 and from January to February, 2024), which aligns with its known reproductive period. Specifically, the mating event occurred in March 2023, followed by observations of courtship behavior in January 2024. While the population dynamics and impact of fisheries remain poorly understood, the species is frequently captured by traditional fishing fleets and traded locally. This reiterates the importance of the local Marine Protected Areas (MPAs) and underlines the urgency for their expansion, alongside the need for further studies that support specific management actions. freeranging free ranging waters notake no take region 202 February 2024, 2024 , 2024) period Specifically understood locally MPAs (MPAs expansion actions 20 2
7.
Coleoptera of Brazil: what we knew then and what we know now. Insights from the Catálogo Taxonômico da Fauna do Brasil Brazil now
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Caron, Edilson
; Monné, Marcela L.
; Ferreira, Vinicius S.
; Costa, Cleide
; Cupello, Mario
; Aloquio, Sergio
; Linzmeier, Adelita M.
; Vaz-de-Mello, Fernando Z.
; Leivas, Fernando W.T.
; Souza-Gonçalves, Igor
; Mermudes, José R.M.
; Almeida, Lúcia M.
; Moura, Luciano de A.
; Ferreira Júnior, Nelson
; Grossi, Paschoal C.
; Vanin, Sergio A.
; Ślipiński, Adam
; Anichtchenko, Alexander
; Newton, Alfred F.
; Sampaio, Aline
; Carelli, Allan
; Puker, Anderson
; Ferreira, André da S.
; Fernandes, André S.
; Roza, André S.
; Cline, Andrew
; Sampaio, Brunno H.L.
; Clarkson, Bruno
; Castro, Camila F. de
; Bicho, Carla de L.
; Benetti, César J.
; Ribeiro-Costa, Cibele S.
; Lopes-Andrade, Cristiano
; Manfio, Daiara
; Colpani, Daniara
; Basílio, Daniel S.
; Bená, Daniela de C.
; Pollock, Darren A.
; Souza, Diego de S.
; Rodrigues, Diego F.
; Chandler, Donald S.
; Nascimento, Elynton A. do
; Spiessberger, Erich L.
; Agrain, Federico A.
; Barbosa, Felipe F.
; Shockley, Floyd
; Nascimento, Francisco E. de L.
; Biffi, Gabriel
; Powell, Gareth S.
; Morse, Geoffrey E.
; Flores, Gustavo E.
; Escalona, Hermes
; Quintino, Hingrid Y.S.
; Rainho, Hugo L.
; Maddalena, Italo S.C.P.
; Hájek, Jiří
; McHugh, Joseph V.
; Botero, Juan P.
; Fuhrmann, Juares
; Churata-Salcedo, Julissa M.
; Vieira, Letícia M.
; Silveira, Luiz F.L. da
; Cruz, Luiza S. da
; Sekerka, Lukás
; Bologna, Marco A.
; Bevilaqua, Marcus V.O.
; Passos, Maria I.
; Chamorro, Maria L.
; Cherman, Mariana A.
; Bento, Matheus
; Gimmel, Matthew
; Segura, Melissa O.
; Ivie, Michael A.
; Thomas, Michael C.
; Monné, Miguel A.
; Lord, Nathan
; Hamada, Neusa
; Degallier, Nicolas
; Santos, Paula B. dos
; Duarte, Paulo R.M.
; Gnaspini, Pedro
; Bulirsch, Petr
; Regalin, Renato
; Leschen, Richard A.B.
; Constantin, Robert
; Corrêa, Rodrigo C.
; Gerstmeier, Roland
; Rosa, Simone P.
; Campos, Stéphanie V.N.
; Peck, Stewart B.
; Pacheco, Thaynara L.
; Polizei, Thiago T.S.
; McElrath, Thomas C.
; Grzymala, Traci L.
; Smith, Trevor R.
; Costa-Silva, Vinicius da
; Sandoval-Gómez, Vivian E.
; Sousa, Wesley O. de
; Tomaszewska, Wioletta
.
ABSTRACT In 2000, Cleide Costa published a paper presenting the state of knowledge of the Neotropical Coleopte ra, with a focus on the Brazilian fauna. Twenty-four years later, thanks to the development of the Coleoptera section of the Taxonomic Catalog of the Brazilian Fauna (CTFB - Catálogo Taxonômico da Fauna do Brasil) through the collaboration of 100 coleopterists from all over the globe, we can build on Costa’s work and present an updated overview of the state of knowledge of the beetles from Brazil. There are currently 35,699 species in 4,958 genera and 116 families known to occur in the country, including representatives of all extant suborders and superfamilies. Our data show that the Brazilian beetle fauna is the richest on the planet, concentrating 9% of the world species diversity, with some estimates accounting to up to 15% of the global total. The most diverse family in numbers of genera is Cerambycidae (1,056 genera), while in number of species it is Chrysomelidae (6,079 species). Conotrachelus Dejean, 1835 (Curculionidae) is the most species-rich genus, with 570 species. The French entomologist Maurice Pic is the author who has contributed the most to the naming of species recorded from Brazil, with 1,794 valid names in 36 families, whereas the Brazilians Ubirajara R. Martins and Maria Helena M. Galileo are the only ones among the top-ten authors to have named species in the 21st century. Currently, approximately 144 new species of Brazilian beetles are described each year, and this average is projected to increase in the next decade to 180 species per year, or about one new Brazilian beetle every two days. 2000 ra Twentyfour Twenty four later CTFB Brasil 10 globe Costas s Brazil 35699 35 699 35,69 4958 4 958 4,95 11 country superfamilies planet 9 diversity 15 total 1,056 1056 1 056 (1,05 genera, , genera) 6,079 6079 6 079 (6,07 . species) Dejean 183 Curculionidae (Curculionidae speciesrich rich genus 57 1794 794 1,79 3 R M topten top ten st century Currently 14 year 18 days 200 3569 69 35,6 495 95 4,9 1,05 105 05 (1,0 6,07 607 07 (6,0 5 179 79 1,7 20 356 35, 49 4, 1,0 0 (1, 6,0 60 (6, 17 7 1, 2 (1 6, (6 (
8.
In silico analysis of non-structural protein 12 sequences from SARS-COV-2 found in Manaus, Amazonas, Brazil, reveals mutations linked to higher transmissibility nonstructural non structural 1 SARSCOV2 SARSCOV SARS COV 2 SARS-COV- Manaus Amazonas Brazil SARS-COV
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ZANCHI, FERNANDO B.
; FERREIRA, GABRIEL EDUARDO M.
; MARIÚBA, LUIS ANDRÉ M.
; GLÓRIA, JULIANE C.
; NASCIMENTO, VALDINETE A. DO
; SOUZA, VICTOR C. DE
; CORADO, ANDRÉ DE LIMA G.
; NASCIMENTO, FERNANDA O. DO
; COSTA, ÁGATHA KÉLLY A. DA
; DUARTE, DÉBORA CAMILA G.
; SILVA, GEORGE ALLAN V. DA
; MEJÍA, MATILDE DEL CARMEN C.
; PESSOA, KARINA P.
; GONÇALVES, LUCIANA MARA F.
; BRANDÃO, MARIA JÚLIA P.
; JESUS, MICHELE S. DE
; SILVA, MARINEIDE S. DA
; COSTA, CRISTIANO F. DA
; NAVECA, FELIPE G.
.
Abstract The disease coronavirus COVID-19 has been the cause of millions of deaths worldwide. Among the proteins of SARS-CoV-2, non-structural protein 12 (NSP12) plays a key role during COVID infection and is part of the RNA-dependent RNA polymerase complex. The monitoring of NSP12 polymorphisms is extremely important for the design of new antiviral drugs and monitoring of viral evolution. This study analyzed the NSP12 mutations detected in circulating SARS-CoV-2 during the years 2020 to 2022 in the population of the city of Manaus, Amazonas, Brazil. The most frequent mutations found were P323L and G671S. Reports in the literature indicate that these mutations are related to transmissibility efficiency, which may have contributed to the extremely high numbers of cases in this location. In addition, two mutations described here (E796D and R914K) are close and have RMSD that is similar to the mutations M794V and N911K, which have been described in the literature as influential on the performance of the NSP12 enzyme. These data demonstrate the need to monitor the emergence of new mutations in NSP12 in order to better understand their consequences for the treatments currently used and in the design of new drugs. COVID19 19 COVID-1 worldwide SARSCoV2, SARSCoV2 SARSCoV SARS CoV 2, 2 nonstructural non structural 1 NSP (NSP12 RNAdependent dependent complex NSP1 evolution SARS-CoV- 202 Manaus Amazonas Brazil PL P L G671S GS G S efficiency location addition E796D ED E D R914K RK R K MV M V N911K NK N enzyme COVID1 COVID- (NSP1 SARS-CoV 20 (NSP
9.
Prognostic implications of the ID1 expression in acute myeloid leukemia patients treated in a resource-constrained setting ID resourceconstrained resource constrained
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Lima, Aleide S.
; Bezerra, Matheus F.
; Moreira-Aguiar, Amanda
; Weinhäuser, Isabel
; Santos, Bianca L.
; Falcão, Raul M.
; Salustiano-Bandeira, Maria L.
; Franca-Neto, Pedro L.
; Lima, Marinus M.
; Saldanha-Araujo, Felipe
; Coelho-Silva, Juan L.
; Pereira-Martins, Diego A.
; Bezerra, Marcos A.
; Lucena-Araujo, Antonio R.
.
Abstract Introduction The aberrant expression of the inhibitor of DNA binding (ID1) gene has been frequently associated with the leukemogenesis and prognostication acute myeloid leukemia (AML), although its clinical importance has never been investigated in patients treated outside well-controlled clinical trials. Methods Using quantitative real-time polymerase chain reaction, we investigated the role of the ID1 expression in the clinical outcomes of non-selected patients with acute myeloid leukemia treated in a real-life setting. Results Overall, 128 patients were enrolled. Patients with high ID1 expression had a lower 3-year overall survival (OS) rate of 9%, with the 95% confidence interval (95%CI) at 3 to 20%, compared to patients with a low ID1 expression (22%, 95%CI: 11 - 34%) (p = 0.037), although these findings did not retain significance after adjustment (hazard ratio (HR): 1.5, 95%CI: 0.98 - 2.28; p = 0.057). The ID1 expression had no impact on post-induction outcomes (disease-free survival, p = 0.648; cumulative incidence of relapse, p = 0.584). Conclusions Although we are aware thar our data are confronted with many variables that cannot be fully controlled, including drug unavailability, risk-adapted treatment, comorbidities and the time from diagnosis to treatment initiation, we are firm believers that such an initiative can provide more realistic data on understudied populations, in particular those from low- and middle-income countries. ID (ID1 AML, AML , (AML) wellcontrolled well controlled trials realtime real reaction nonselected non selected reallife life setting Overall 12 enrolled 3year year OS (OS 9 9% 95 95%CI 95CI CI (95%CI 20 20% 22%, 22 (22% 1 34% 34 0.037, 0037 0.037 0 037 0.037) hazard HR (HR) 15 5 1.5 098 98 0.9 2.28 228 2 28 0.057. 0057 0.057 . 057 0.057) postinduction post induction diseasefree disease free 0.648 0648 648 relapse 0.584. 0584 0.584 584 0.584) unavailability riskadapted risk adapted initiation populations middleincome middle income countries (ID (AML 22% (22 003 0.03 03 (HR 1. 09 0. 2.2 005 0.05 05 0.64 064 64 058 0.58 58 (2 00 0.0 2. 0.6 06 6 0.5 (
10.
Prospecting plant growth-promoting rhizobacteria in grapevines in the São Francisco Valley growthpromoting growth promoting
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Mendes Júnior, José P.
; Fracetto, Giselle G. M.
; Fracetto, Felipe J. C.
; Silva, Davi J.
; Lira Junior, Mario de A.
; Barros, Felipe M. do R.
.
RESUMO A viticultura é a principal produção agrícola do Vale do São Francisco, Brasil; no entanto, os sistemas agrícolas de manejo dos solos requerem grandes volumes de fertilizantes que podem contribuir para as alterações climáticas. Portanto, foi relatado que o uso de rizobactérias promotoras de crescimento de plantas (RPCP) reduz ou substitui insumos vegetais. Este estudo teve como objetivo avaliar as bactérias da rizosfera de Vitis vinifera cultivada no Vale do São Francisco, em Pernambuco, Brasil, e caracterizar os mecanismos que promovem o crescimento das plantas. As RPCP com maior potencial biotecnológico foram identificadas por meio de sequenciamento. As bactérias foram isoladas em meio de cultura Agar Triptona de Soja (TSA) inoculado com 100 µL de uma diluição seriada. Os isolados obtidos foram caracterizados fenotipicamente e testados quanto à capacidade de solubilizar fosfato, promover fixação biológica de nitrogênio (FBN) e produzir ácido indol-3 acético (AIA), biofilmes e fatores antibióticos contra Xanthomonas campestris pv. vitícola. Um total de 423 bactérias foi obtido, das quais 99 apresentaram resultados positivos para pelo menos um dos mecanismos de promoção de crescimento, representando 6,85% para solubilizadores de fosfato, 0,74% para FBN, 5,7% para sintetizadores de AIA, 11,27% para produtores de biofilme e 4,01% para promoção de antibiose contra X. campestris pv. vitícola. Os isolados 3.19 e 31.14; 3.17 e 17.04; 5.35 e 5.42; e 5.37 identificados como Stenotrophomonas, Bacillus, Pseudomonas e Clostridium, respectivamente, apresentaram potencial biotecnológico para futuros experimentos para promover o crescimento de videiras. Francisco Brasil entanto climáticas Portanto (RPCP vegetais Pernambuco sequenciamento TSA (TSA 10 seriada fosfato FBN (FBN indol3 indol 3 indol- AIA , (AIA) pv vitícola 42 obtido 9 685 6 85 6,85 074 0 74 0,74 57 5 7 5,7 1127 11 27 11,27 401 4 01 4,01 X 319 19 3.1 31.14 3114 31 14 317 17 17.04 1704 04 535 35 5.3 5.42 542 537 37 Stenotrophomonas Bacillus Clostridium respectivamente videiras 1 (AIA 68 8 6,8 07 0,7 5, 112 2 11,2 40 4,0 3. 31.1 311 17.0 170 53 5. 5.4 54 6, 0, 11, 4, 31. 17.
ABSTRACT Viticulture is the main agricultural production in the São Francisco Valley, Brazil; however, farm soil management systems require large volumes of fertilizer that could contribute to climate change. Therefore, using plant growth-promoting rhizobacteria (PGPR) has been reported to reduce or replace plant input. This study aimed to evaluate the bacteria in the rhizosphere of Vitis vinifera cultivated in the São Francisco Valley in Pernambuco, Brazil, and to characterize the mechanisms that promote plant growth. The PGPR with the greatest biotechnological potential was identified using sequencing. The bacteria were isolated from tryptic soy agar (TSA) culture medium inoculated with 100 µL of a serial dilution. The isolates obtained were characterized phenotypically and tested for their ability to solubilize phosphate, promote biological nitrogen fixation (BNF), and produce indole-3 acetic acid (IAA), biofilms, and antibiotic factors against Xanthomonas campestris pv. viticola. A total of 423 bacteria were obtained, of which 99 presented positive results for at least one of the growth-promotion mechanisms, representing 6.85% for phosphate solubilizers, 0.74% for BNF, 5.7% for IAA synthesizers, 11.27% for biofilm producers, and 4.01% for promoting antibiosis against X. campestris pv. viticola. Isolates 3.19 and 31.14; 3.17 and 17.04; 5.35 and 5.42; and 5.37 identified as Stenotrophomonas, Bacillus, Pseudomonas, and Clostridium, respectively, presented a biotechnological potential for future experiments to promote vine growth. Brazil however change Therefore growthpromoting growth (PGPR input Pernambuco sequencing TSA (TSA 10 dilution BNF , (BNF) indole3 indole 3 indole- IAA, (IAA) biofilms pv viticola 42 9 growthpromotion promotion 685 6 85 6.85 solubilizers 074 0 74 0.74 57 5 7 5.7 synthesizers 1127 11 27 11.27 producers 401 4 01 4.01 X 319 19 3.1 31.14 3114 31 14 317 17 17.04 1704 04 535 35 5.3 5.42 542 537 37 Stenotrophomonas Bacillus Pseudomonas Clostridium respectively 1 (BNF (IAA 68 8 6.8 07 0.7 5. 112 2 11.2 40 4.0 3. 31.1 311 17.0 170 53 5.4 54 6. 0. 11. 4. 31. 17.
11.
Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil Brasil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
; Slipinski, Adam
; Linzmeier, Adelita M.
; Calor, Adolfo R.
; Garda, Adrian A.
; Kury, Adriano B.
; Fernandes, Agatha C.S.
; Agudo-Padrón, Aisur I.
; Akama, Alberto
; Silva Neto, Alberto M. da
; Burbano, Alejandro L.
; Menezes, Aleksandra
; Pereira-Colavite, Alessandre
; Anichtchenko, Alexander
; Lees, Alexander C.
; Bezerra, Alexandra M.R.
; Domahovski, Alexandre C.
; Pimenta, Alexandre D.
; Aleixo, Alexandre L.P.
; Marceniuk, Alexandre P.
; Paula, Alexandre S. de
; Somavilla, Alexandre
; Specht, Alexandre
; Camargo, Alexssandro
; Newton, Alfred F.
; Silva, Aline A.S. da
; Santos, Aline B. dos
; Tassi, Aline D.
; Aragão, Allan C.
; Santos, Allan P.M.
; Migotto, Alvaro E.
; Mendes, Amanda C.
; Cunha, Amanda
; Chagas Júnior, Amazonas
; Sousa, Ana A.T. de
; Pavan, Ana C.
; Almeida, Ana C.S.
; Peronti, Ana L.B.G.
; Henriques-Oliveira, Ana L.
; Prudente, Ana L.
; Tourinho, Ana L.
; Pes, Ana M.O.
; Carmignotto, Ana P.
; Wengrat, Ana P.G. da Silva
; Dornellas, Ana P.S.
; Molin, Anamaria Dal
; Puker, Anderson
; Morandini, André C.
; Ferreira, André da S.
; Martins, André L.
; Esteves, André M.
; Fernandes, André S.
; Roza, André S.
; Köhler, Andreas
; Paladini, Andressa
; Andrade, Andrey J. de
; Pinto, Ângelo P.
; Salles, Anna C. de A.
; Gondim, Anne I.
; Amaral, Antonia C.Z.
; Rondón, Antonio A.A.
; Brescovit, Antonio
; Lofego, Antônio C.
; Marques, Antonio C.
; Macedo, Antonio
; Andriolo, Artur
; Henriques, Augusto L.
; Ferreira Júnior, Augusto L.
; Lima, Aurino F. de
; Barros, Ávyla R. de A.
; Brito, Ayrton do R.
; Romera, Bárbara L.V.
; Vasconcelos, Beatriz M.C. de
; Frable, Benjamin W.
; Santos, Bernardo F.
; Ferraz, Bernardo R.
; Rosa, Brunno B.
; Sampaio, Brunno H.L.
; Bellini, Bruno C.
; Clarkson, Bruno
; Oliveira, Bruno G. de
; Corrêa, Caio C.D.
; Martins, Caleb C.
; Castro-Guedes, Camila F. de
; Souto, Camilla
; Bicho, Carla de L.
; Cunha, Carlo M.
; Barboza, Carlos A. de M.
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; Barreto, Carlos
; Santana, Carlos D.C.M. de
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; Margaría, Cecilia B.
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; Digiani, Celina
; Haddad, Célio F.B.
; Azevedo, Celso O.
; Benetti, Cesar J.
; Santos, Charles M.D. dos
; Bartlett, Charles R.
; Bonvicino, Cibele
; Ribeiro-Costa, Cibele S.
; Santos, Cinthya S.G.
; Justino, Cíntia E.L.
; Canedo, Clarissa
; Bonecker, Claudia C.
; Santos, Cláudia P.
; Carvalho, Claudio J.B. de
; Gonçalves, Clayton C.
; Galvão, Cleber
; Costa, Cleide
; Oliveira, Cléo D.C. de
; Schwertner, Cristiano F.
; Andrade, Cristiano L.
; Pereira, Cristiano M.
; Sampaio, Cristiano
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; Lucena, Daercio A. de A.
; Manfio, Daiara
; Amorim, Dalton de S.
; Queiroz, Dalva L. de
; Queiroz, Dalva L. de
; Colpani, Daniara
; Abbate, Daniel
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; Burckhardt, Daniel
; Cavallari, Daniel C.
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; Basílio, Daniel S.
; Bená, Daniela de C.
; Toledo, Daniela G.P. de
; Takiya, Daniela M.
; Fernandes, Daniell R.R.
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; Pollock, Darren A.
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; Gibson, David I.
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; Marques, Dayse W.A.
; Lucatelli, Débora
; Garcia, Deivys M.A.
; Baêta, Délio
; Ferreira, Denise N.M.
; Rueda-Ramírez, Diana
; Fachin, Diego A.
; Souza, Diego de S.
; Rodrigues, Diego F.
; Pádua, Diego G. de
; Barbosa, Diego N.
; Dolibaina, Diego R.
; Amaral, Diogo C.
; Chandler, Donald S.
; Maccagnan, Douglas H.B.
; Caron, Edilson
; Carvalho, Edrielly
; Adriano, Edson A.
; Abreu Júnior, Edson F. de
; Pereira, Edson H.L.
; Viegas, Eduarda F.G.
; Carneiro, Eduardo
; Colley, Eduardo
; Eizirik, Eduardo
; Santos, Eduardo F. dos
; Shimbori, Eduardo M.
; Suárez-Morales, Eduardo
; Arruda, Eliane P. de
; Chiquito, Elisandra A.
; Lima, Élison F.B.
; Castro, Elizeu B. de
; Orlandin, Elton
; Nascimento, Elynton A. do
; Razzolini, Emanuel
; Gama, Emanuel R.R.
; Araujo, Enilma M. de
; Nishiyama, Eric Y.
; Spiessberger, Erich L.
; Santos, Érika C.L. dos
; Contreras, Eugenia F.
; Galati, Eunice A.B.
; Oliveira Junior, Evaldo C. de
; Gallardo, Fabiana
; Hernandes, Fabio A.
; Lansac-Tôha, Fábio A.
; Pitombo, Fabio B.
; Dario, Fabio Di
; Santos, Fábio L. dos
; Mauro, Fabio
; Nascimento, Fabio O. do
; Olmos, Fabio
; Amaral, Fabio R.
; Schunck, Fabio
; Godoi, Fábio S. P. de
; Machado, Fabrizio M.
; Barbo, Fausto E.
; Agrain, Federico A.
; Ribeiro, Felipe B.
; Moreira, Felipe F.F.
; Barbosa, Felipe F.
; Silva, Fenanda S.
; Cavalcanti, Fernanda F.
; Straube, Fernando C.
; Carbayo, Fernando
; Carvalho Filho, Fernando
; Zanella, Fernando C.V.
; Jacinavicius, Fernando de C.
; Farache, Fernando H.A.
; Leivas, Fernando
; Dias, Fernando M.S.
; Mantellato, Fernando
; Vaz-de-Mello, Fernando Z.
; Gudin, Filipe M.
; Albuquerque, Flávio
; Molina, Flavio B.
; Passos, Flávio D.
; Shockley, Floyd W.
; Pinheiro, Francielly F.
; Mello, Francisco de A.G. de
; Nascimento, Francisco E. de L.
; Franco, Francisco L.
; Oliveira, Francisco L. de
; Melo, Francisco T. de V.
; Quijano, Freddy R.B.
; Salles, Frederico F.
; Biffi, Gabriel
; Queiroz, Gabriel C.
; Bizarro, Gabriel L.
; Hrycyna, Gabriela
; Leviski, Gabriela
; Powell, Gareth S.
; Santos, Geane B. dos
; Morse, Geoffrey E.
; Brown, George
; Mattox, George M.T.
; Zimbrão, Geraldo
; Carvalho, Gervásio S.
; Miranda, Gil F.G.
; Moraes, Gilberto J. de
; Lourido, Gilcélia M.
; Neves, Gilmar P.
; Moreira, Gilson R.P.
; Montingelli, Giovanna G.
; Maurício, Giovanni N.
; Marconato, Gláucia
; Lopez, Guilherme E.L.
; Silva, Guilherme L. da
; Muricy, Guilherme
; Brito, Guilherme R.R.
; Garbino, Guilherme S.T.
; Flores, Gustavo E.
; Graciolli, Gustavo
; Libardi, Gustavo S.
; Proctor, Heather C.
; Gil-Santana, Helcio R.
; Varella, Henrique R.
; Escalona, Hermes E.
; Schmitz, Hermes J.
; Rodrigues, Higor D.D.
; Galvão Filho, Hilton de C.
; Quintino, Hingrid Y.S.
; Pinto, Hudson A.
; Rainho, Hugo L.
; Miyahira, Igor C.
; Gonçalves, Igor de S.
; Martins, Inês X.
; Cardoso, Irene A.
; Oliveira, Ismael B. de
; Franz, Ismael
; Fernandes, Itanna O.
; Golfetti, Ivan F.
; S. Campos-Filho, Ivanklin
; Oliveira, Ivo de S.
; Delabie, Jacques H.C.
; Oliveira, Jader de
; Prando, Jadila S.
; Patton, James L.
; Bitencourt, Jamille de A.
; Silva, Janaina M.
; Santos, Jandir C.
; Arruda, Janine O.
; Valderrama, Jefferson S.
; Dalapicolla, Jeronymo
; Oliveira, Jéssica P.
; Hájek, Jiri
; Morselli, João P.
; Narita, João P.
; Martin, João P.I.
; Grazia, Jocélia
; McHugh, Joe
; Cherem, Jorge J.
; Farias Júnior, José A.S.
; Fernandes, Jose A.M.
; Pacheco, José F.
; Birindelli, José L.O.
; Rezende, José M.
; Avendaño, Jose M.
; Duarte, José M. Barbanti
; Ribeiro, José R. Inácio
; Mermudes, José R.M.
; Pujol-Luz, José R.
; Santos, Josenilson R. dos
; Câmara, Josenir T.
; Teixeira, Joyce A.
; Prado, Joyce R. do
; Botero, Juan P.
; Almeida, Julia C.
; Kohler, Julia
; Gonçalves, Julia P.
; Beneti, Julia S.
; Donahue, Julian P.
; Alvim, Juliana
; Almeida, Juliana C.
; Segadilha, Juliana L.
; Wingert, Juliana M.
; Barbosa, Julianna F.
; Ferrer, Juliano
; Santos, Juliano F. dos
; Kuabara, Kamila M.D.
; Nascimento, Karine B.
; Schoeninger, Karine
; Campião, Karla M.
; Soares, Karla
; Zilch, Kássia
; Barão, Kim R.
; Teixeira, Larissa
; Sousa, Laura D. do N.M. de
; Dumas, Leandro L.
; Vieira, Leandro M.
; Azevedo, Leonardo H.G.
; Carvalho, Leonardo S.
; Souza, Leonardo S. de
; Rocha, Leonardo S.G.
; Bernardi, Leopoldo F.O.
; Vieira, Letícia M.
; Johann, Liana
; Salvatierra, Lidianne
; Oliveira, Livia de M.
; Loureiro, Lourdes M.A. El-moor
; Barreto, Luana B.
; Barros, Luana M.
; Lecci, Lucas
; Camargos, Lucas M. de
; Lima, Lucas R.C.
; Almeida, Lucia M.
; Martins, Luciana R.
; Marinoni, Luciane
; Moura, Luciano de A.
; Lima, Luciano
; Naka, Luciano N.
; Miranda, Lucília S.
; Salik, Lucy M.
; Bezerra, Luis E.A.
; Silveira, Luis F.
; Campos, Luiz A.
; Castro, Luiz A.S. de
; Pinho, Luiz C.
; Silveira, Luiz F.L.
; Iniesta, Luiz F.M.
; Tencatt, Luiz F.C.
; Simone, Luiz R.L.
; Malabarba, Luiz R.
; Cruz, Luiza S. da
; Sekerka, Lukas
; Barros, Lurdiana D.
; Santos, Luziany Q.
; Skoracki, Maciej
; Correia, Maira A.
; Uchoa, Manoel A.
; Andrade, Manuella F.G.
; Hermes, Marcel G.
; Miranda, Marcel S.
; Araújo, Marcel S. de
; Monné, Marcela L.
; Labruna, Marcelo B.
; Santis, Marcelo D. de
; Duarte, Marcelo
; Knoff, Marcelo
; Nogueira, Marcelo
; Britto, Marcelo R. de
; Melo, Marcelo R.S. de
; Carvalho, Marcelo R. de
; Tavares, Marcelo T.
; Kitahara, Marcelo V.
; Justo, Marcia C.N.
; Botelho, Marcia J.C.
; Couri, Márcia S.
; Borges-Martins, Márcio
; Felix, Márcio
; Oliveira, Marcio L. de
; Bologna, Marco A.
; Gottschalk, Marco S.
; Tavares, Marcos D.S.
; Lhano, Marcos G.
; Bevilaqua, Marcus
; Santos, Marcus T.T.
; Domingues, Marcus V.
; Sallum, Maria A.M.
; Digiani, María C.
; Santarém, Maria C.A.
; Nascimento, Maria C. do
; Becerril, María de los A.M.
; Santos, Maria E.A. dos
; Passos, Maria I. da S. dos
; Felippe-Bauer, Maria L.
; Cherman, Mariana A.
; Terossi, Mariana
; Bartz, Marie L.C.
; Barbosa, Marina F. de C.
; Loeb, Marina V.
; Cohn-Haft, Mario
; Cupello, Mario
; Martins, Marlúcia B.
; Christofersen, Martin L.
; Bento, Matheus
; Rocha, Matheus dos S.
; Martins, Maurício L.
; Segura, Melissa O.
; Cardenas, Melissa Q.
; Duarte, Mércia E.
; Ivie, Michael A.
; Mincarone, Michael M.
; Borges, Michela
; Monné, Miguel A.
; Casagrande, Mirna M.
; Fernandez, Monica A.
; Piovesan, Mônica
; Menezes, Naércio A.
; Benaim, Natalia P.
; Reategui, Natália S.
; Pedro, Natan C.
; Pecly, Nathalia H.
; Ferreira Júnior, Nelson
; Silva Júnior, Nelson J. da
; Perioto, Nelson W.
; Hamada, Neusa
; Degallier, Nicolas
; Chao, Ning L.
; Ferla, Noeli J.
; Mielke, Olaf H.H.
; Evangelista, Olivia
; Shibatta, Oscar A.
; Oliveira, Otto M.P.
; Albornoz, Pablo C.L.
; Dellapé, Pablo M.
; Gonçalves, Pablo R.
; Shimabukuro, Paloma H.F.
; Grossi, Paschoal
; Rodrigues, Patrícia E. da S.
; Lima, Patricia O.V.
; Velazco, Paul
; Santos, Paula B. dos
; Araújo, Paula B.
; Silva, Paula K.R.
; Riccardi, Paula R.
; Garcia, Paulo C. de A.
; Passos, Paulo G.H.
; Corgosinho, Paulo H.C.
; Lucinda, Paulo
; Costa, Paulo M.S.
; Alves, Paulo P.
; Roth, Paulo R. de O.
; Coelho, Paulo R.S.
; Duarte, Paulo R.M.
; Carvalho, Pedro F. de
; Gnaspini, Pedro
; Souza-Dias, Pedro G.B.
; Linardi, Pedro M.
; Bartholomay, Pedro R.
; Demite, Peterson R.
; Bulirsch, Petr
; Boll, Piter K.
; Pereira, Rachel M.M.
; Silva, Rafael A.P.F.
; Moura, Rafael B. de
; Boldrini, Rafael
; Silva, Rafaela A. da
; Falaschi, Rafaela L.
; Cordeiro, Ralf T.S.
; Mello, Ramon J.C.L.
; Singer, Randal A.
; Querino, Ranyse B.
; Heleodoro, Raphael A.
; Castilho, Raphael de C.
; Constantino, Reginaldo
; Guedes, Reinaldo C.
; Carrenho, Renan
; Gomes, Renata S.
; Gregorin, Renato
; Machado, Renato J.P.
; Bérnils, Renato S.
; Capellari, Renato S.
; Silva, Ricardo B.
; Kawada, Ricardo
; Dias, Ricardo M.
; Siewert, Ricardo
; Brugnera, Ricaro
; Leschen, Richard A.B.
; Constantin, Robert
; Robbins, Robert
; Pinto, Roberta R.
; Reis, Roberto E. dos
; Ramos, Robson T. da C.
; Cavichioli, Rodney R.
; Barros, Rodolfo C. de
; Caires, Rodrigo A.
; Salvador, Rodrigo B.
; Marques, Rodrigo C.
; Araújo, Rodrigo C.
; Araujo, Rodrigo de O.
; Dios, Rodrigo de V.P.
; Johnsson, Rodrigo
; Feitosa, Rodrigo M.
; Hutchings, Roger W.
; Lara, Rogéria I.R.
; Rossi, Rogério V.
; Gerstmeier, Roland
; Ochoa, Ronald
; Hutchings, Rosa S.G.
; Ale-Rocha, Rosaly
; Rocha, Rosana M. da
; Tidon, Rosana
; Brito, Rosangela
; Pellens, Roseli
; Santos, Sabrina R. dos
; Santos, Sandra D. dos
; Paiva, Sandra V.
; Santos, Sandro
; Oliveira, Sarah S. de
; Costa, Sávio C.
; Gardner, Scott L.
; Leal, Sebastián A. Muñoz
; Aloquio, Sergio
; Bonecker, Sergio L.C.
; Bueno, Sergio L. de S.
; Almeida, Sérgio M. de
; Stampar, Sérgio N.
; Andena, Sérgio R.
; Posso, Sergio R.
; Lima, Sheila P.
; Gadelha, Sian de S.
; Thiengo, Silvana C.
; Cohen, Simone C.
; Brandão, Simone N.
; Rosa, Simone P.
; Ribeiro, Síria L.B.
; Letana, Sócrates D.
; Santos, Sonia B. dos
; Andrade, Sonia C.S.
; Dávila, Stephane
; Vaz, Stéphanie
; Peck, Stewart B.
; Christo, Susete W.
; Cunha, Suzan B.Z.
; Gomes, Suzete R.
; Duarte, Tácio
; Madeira-Ott, Taís
; Marques, Taísa
; Roell, Talita
; Lima, Tarcilla C. de
; Sepulveda, Tatiana A.
; Maria, Tatiana F.
; Ruschel, Tatiana P.
; Rodrigues, Thaiana
; Marinho, Thais A.
; Almeida, Thaís M. de
; Miranda, Thaís P.
; Freitas, Thales R.O.
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; Pacheco, Thaynara L.
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; Alvarenga, Thiago M.
; Carvalho, Thiago R. de
; Polizei, Thiago T.S.
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; Henry, Thomas
; Pikart, Tiago G.
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; Carvalho, Tiago P.
; Lotufo, Tito M. da C.
; Caramaschi, Ulisses
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; Pardiñas, Ulyses F.J.
; Maia, Valéria C.
; Tavares, Valeria
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; Amaral, Vanessa S. do
; Silva, Vera C.
; Wolff, Vera R. dos S.
; Slobodian, Verônica
; Silva, Vinícius B. da
; Espíndola, Vinicius C.
; Costa-Silva, Vinicius da
; Bertaco, Vinicius de A.
; Padula, Vinícius
; Ferreira, Vinicius S.
; Silva, Vitor C.P. da
; Piacentini, Vítor de Q.
; Sandoval-Gómez, Vivian E.
; Trevine, Vivian
; Sousa, Viviane R.
; Sant’Anna, Vivianne B. de
; Mathis, Wayne N.
; Souza, Wesley de O.
; Colombo, Wesley D.
; Tomaszewska, Wioletta
; Wosiacki, Wolmar B.
; Ovando, Ximena M.C.
; Leite, Yuri L.R.
.
ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others. publications problem uptodate up date classifications context exception (CTFB http//fauna.jbrj.gov.br/, httpfaunajbrjgovbr http //fauna.jbrj.gov.br/ , jbrj gov br (http://fauna.jbrj.gov.br/) 2015 Brazil 80 specialists 1 2024 133691 133 691 133,69 125138 125 138 125,13 82.3%, 823 82 3 (82.3% 102000 102 000 102,00 7.69%, 769 7 69 (7.69% 11000 11 11,00 . 3,567 3567 567 (3,56 2,292 2292 2 292 (2,29 1,833 1833 833 (1,83 1,447 1447 447 (1,44 1000 1,00 831 (83 628 (62 606 (60 520 (52 50 users science health biology law anthropology education others http//fauna.jbrj.gov.br/ faunajbrjgovbr //fauna.jbrj.gov.br (http://fauna.jbrj.gov.br/ 201 8 202 13369 13 133,6 12513 12 125,1 82.3% (82.3 10200 10 00 102,0 7.69% 76 6 (7.69 1100 11,0 3,56 356 56 (3,5 2,29 229 29 (2,2 1,83 183 83 (1,8 1,44 144 44 (1,4 100 1,0 (8 62 (6 60 52 (5 5 http//fauna.jbrj.gov.br (http://fauna.jbrj.gov.br 20 1336 133, 1251 125, 82.3 (82. 1020 0 102, 7.69 (7.6 110 11, 3,5 35 (3, 2,2 22 (2, 1,8 18 (1, 1,4 14 4 ( 82. (82 7.6 (7. 3, (3 2, (2 (1 7. (7
12.
Black Carbon and Particulate Matter Concentrations: Air Pollution Levels in Rio de Janeiro, Brazil Concentrations Janeiro
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Cruz, Alex H. De La
; Silva, Luis Fhernando M. da
; Silva, Luiz Felipe M.
; Anjos, Vanessa A. dos
; Godoi, Ricardo Henrique M.
; Gioda, Adriana
.
This research explores the concentration of black carbon (BC) in particulate matter (PM10 and PM2.5) from ten monitoring stations in the Metropolitan Region of Rio de Janeiro (MRRJ), Bonsucesso (BS), Botafogo (BOT), Copacabana (COP), Gavea (GAV), Gericino (GER), Lagoa (LAG), Recreio dos Bandeirantes (REC), Santa Cruz (SC), Castelo (CAS) and Urca (URC), covering a range of pollution sources (vehicular, industrial, and residential). PM samples were collected using filter units every week from January 2018 to December 2019. Results revealed high concentrations of PM10 in BS (86 ± 22 µg m3) and PM2.5 in REC (30 ± 11 µg m3). Likewise, both monitoring stations exceeded the international limits. In 2019, BC in PM10 decreased in the following order: BS > CAS > GER > BOT > SC > GAV. For 2018, BC in PM2.5 decreased as follows REC > LAG > SC, while 2019 REC > GAV > LAG > COP > URC. REC and BS have industrial and commercial activities and intense vehicular traffic. During the period of study, average BC concentrations in PM10 and PM2.5 were 3.3 ± 1.5 and 1.9 ± 0.70 µg m3, respectively. These findings indicate that BC concentrations should be monitored and regulated in locations with high levels of traffic-related air pollution for offering new insights and guiding efforts to minimize emissions and enhance public health. (BC (PM1 PM25 PM2 5 MRRJ, MRRJ , (MRRJ) BS, (BS) BOT, (BOT) COP, (COP) GAV, (GAV) GER, (GER) LAG, (LAG) REC, (REC) (SC) (CAS URC, URC (URC) vehicular, (vehicular residential. residential . residential) 201 PM1 86 (8 2 m3 m PM2. 30 (3 1 m3. Likewise limits order traffic study 33 3 3. 15 1. 19 9 070 0 70 0.7 respectively trafficrelated related health (PM (MRRJ (BS (BOT (COP (GAV (GER (LAG (REC (SC (URC 20 8 ( 07 7 0.
13.
Dereplication of Sclerotiorin-Like Azaphilones Produced by Penicillium meliponae Using LC-MS/MS Analysis and Molecular Networking SclerotiorinLike Sclerotiorin Like LCMS/MS LCMSMS LC MS/MS MS LCMS MSMS
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Peres, Eldrinei G.
; Souza, Mayane P.
; Sousa, Thiago F.
; Silva, Carlos V. A. da
; Barros, André L.
; Silva, Felipe M. A. da
; Costa, Emmanoel V.
; Medeiros, Lívia S. de
; Forim, Moacir R.
; Souza, Afonso D. L. de
; Paz, Weider H. P.
; Silva, Gilvan F. da
; Souza, Antonia Q. L. de
; Koolen, Hector H. F.
.
Journal of the Brazilian Chemical Society
- Journal Metrics
Penicillium meliponae, a recently described and rare species, was isolated as an endophytic fungus from the Amazonian plant Duguetia sthelechantha, and has been proven to be a pigment producer. Considering the high productivity of this species and the lack of data on its chemical composition, the present study aimed to characterize the chemical profile of P. meliponae and evaluate the influence of agitation and the use of different culture media. For this purpose, liquid chromatography coupled with mass spectrometry (LC-MS/MS) and molecular networking were used, allowing the identification of 17 azaphilone molecules with sclerotiorin-like skeletons, becoming the first chemical report of this species. In addition, the different production patterns in the tested culture media were indicative that this species is sensitive to changes in the composition of the carbon source and to the presence of agitation. Furthermore, this work contributes to the fragmentation mechanisms of the different possible structural arrangements for azaphilones of the sclerotiorin type and serves as a repository of information on the gas-phase behavior of this type of metabolite in mass spectrometry experiments and will assist future studies aimed at the discovery of azaphilones. sthelechantha producer P purpose LCMS/MS LCMSMS LC MS/MS MS (LC-MS/MS used 1 sclerotiorinlike like skeletons addition Furthermore gasphase gas phase LCMS MSMS
14.
Evaluation of phosphorus levels in bones and serum of buffaloes (Bubalus bubalis) before and after supplementation with a selective mineral mixture Bubalus bubalis
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Oliveira, Carlos M.C.
; Bomjardim, Henrique A.
; Silveira, Natália S.S.
; Silveira, José Alcides S.
; Faial, Kelson C.F.
; Silva Filho, Ednaldo
; Salvarani, Felipe M.
; Barbosa, José D.
.
RESUMO: Objetivou-se avaliar as concentrações de fósforo (P) no soro e no osso, o percentual de cinzas e a densidade óssea específica em búfalas da Ilha de Marajó antes e após suplementação mineral seletiva. Foram utilizadas 14 búfalas mestiças de Murrah com Mediterrânea, com idades entre 18 e 36 meses. Os valores médios de P, antes da suplementação, no soro, no osso, o percentual de cinzas e a densidade óssea específica foram de 5,68mg/dL±1,18, 16,53%±0,53, 59,95%±1,96 e 1,52g/cm3±0,32, respectivamente, o que demonstra deficiência de P nos animais criados na Ilha de Marajó. Após a suplementação com P por um período de sete meses os valores foram 6,61mg/dl±0,87, 16,90%±0,56 e 60,30%±0,95 e 1,71g/cm3±0,21 respectivamente. Esses resultados caracterizam um aumento significativo nas concentrações de P no soro sanguíneo, na densidade óssea específica e no percentual de P nas cinzas (P<0,05), porém não houve um aumento significativo no percentual de cinzas. O aumento médio nos valores de P no soro e nas cinzas não alcançou patamares de normalidade em todas as búfalas, entretanto 28,6% delas tinham valores normais de P no soro e 50% nas cinzas, 64,3% tinham valores normais da densidade óssea específica. O não restabelecimento, em parte dos animais, das variáveis ósseas e sanguíneas após suplementação com P durante sete meses pode ter ocorrido em virtude da baixa ingestão da mistura mineral e da baixa concentração de P em Brachiaria brizantha cv. Marandu utilizada para alimentação dos animais durante o experimento. RESUMO Objetivouse Objetivou se (P osso seletiva 1 Mediterrânea 3 568mgdL118 mgdL 5 68mg dL mg 5,68mg/dL±1,18 1653053 16 53 0 16,53%±0,53 5995196 59 95 96 59,95%±1,9 152gcm3032 gcm 52g cm3 32 g cm 1,52g/cm3±0,32 respectivamente 661mgdl087 mgdl 6 61mg dl 87 6,61mg/dl±0,87 1690056 90 56 16,90%±0,5 6030095 60 30 60,30%±0,9 171gcm3021 71g 21 1,71g/cm3±0,2 sanguíneo P<0,05, P005 P<0,05 , 05 (P<0,05) 286 28 28,6 50 643 64 64,3 restabelecimento cv experimento 568mgdL11 5,68mg/dL±1,1 165305 16,53%±0,5 599519 9 59,95%±1, 152gcm303 1,52g/cm3±0,3 661mgdl08 8 6,61mg/dl±0,8 169005 16,90%±0, 603009 60,30%±0, 171gcm302 2 1,71g/cm3±0, P00 P<0,0 (P<0,05 28, 64, 568mgdL1 5,68mg/dL±1, 16530 16,53%±0, 59951 59,95%±1 152gcm30 1,52g/cm3±0, 661mgdl0 6,61mg/dl±0, 16900 16,90%±0 60300 60,30%±0 171gcm30 1,71g/cm3±0 P0 P<0, (P<0,0 568mgdL 5,68mg/dL±1 1653 16,53%±0 5995 59,95%± 152gcm3 1,52g/cm3±0 661mgdl 6,61mg/dl±0 1690 16,90%± 6030 60,30%± 171gcm3 1,71g/cm3± P<0 (P<0, 5,68mg/dL± 165 16,53%± 599 59,95% 152gcm 1,52g/cm3± 6,61mg/dl± 169 16,90% 603 60,30% 171gcm 1,71g/cm3 P< (P<0 5,68mg/dL 16,53% 59,95 1,52g/cm3 6,61mg/dl 16,90 60,30 1,71g/cm (P< 16,53 59,9 1,52g/cm 16,9 60,3 16,5 59, 16, 60,
ABSTRACT: This study aimed to evaluate phosphorus (P) concentration in serum and bone, the percentage of ash and the specific bone density of buffaloes on Ilha de Marajó before and after mineral supplementation. For this study, 14 crossbred buffaloes of Murrah and Mediterranean descent aged between 18 and 36 months were used. The average values of P before supplementation in serum and bone, the percentage of bone ash and the specific bone density were 5.68mg/dL±1.18, 16.53%±0.53, 59.95%±1.96 and 1.52g/cm3±0.32, respectively, which demonstrated P deficiency in animals raised on Ilha de Marajó. After supplementation with P for a period of seven months, the values were 6.61mg/dL±0.87, 16.90%±0.56 and 60.30%±0.95 and 1.71g/cm3±0.21, respectively. These results showed a significant increase in P concentration in blood serum, specific bone density and percentage of P in ash (P<0.05), but there was no significant increase in the percentage of ash. The average increase in P in the serum and ash did not reach normal levels in all animals; however, 28.6% of the animals had normal values of P in serum and 50% in the ash, and 64.3% had normal specific bone density values. The nonre-establishment, in some of the animals, of the variables of P serum and bone after supplementation for seven months may have occurred as a result of the low intake of the mineral mixture and by the low concentration of P in the Brachiaria brizantha cv. Marandu used for feeding animals during the experiment. ABSTRACT (P 1 3 568mgdL118 mgdL 5 68mg dL mg 5.68mg/dL±1.18 1653053 16 53 0 16.53%±0.53 5995196 59 95 96 59.95%±1.9 152gcm3032 gcm 52g cm3 32 g cm 1.52g/cm3±0.32 respectively 661mgdL087 6 61mg 87 6.61mg/dL±0.87 1690056 90 56 16.90%±0.5 6030095 60 30 60.30%±0.9 171gcm3021 71g 21 1.71g/cm3±0.21 P<0.05, P005 P<0.05 , 05 (P<0.05) however 286 28 28.6 50 643 64 64.3 nonreestablishment, nonreestablishment nonre establishment, establishment nonre-establishment cv experiment 568mgdL11 5.68mg/dL±1.1 165305 16.53%±0.5 599519 9 59.95%±1. 152gcm303 1.52g/cm3±0.3 661mgdL08 8 6.61mg/dL±0.8 169005 16.90%±0. 603009 60.30%±0. 171gcm302 2 1.71g/cm3±0.2 P00 P<0.0 (P<0.05 28. 64. 568mgdL1 5.68mg/dL±1. 16530 16.53%±0. 59951 59.95%±1 152gcm30 1.52g/cm3±0. 661mgdL0 6.61mg/dL±0. 16900 16.90%±0 60300 60.30%±0 171gcm30 1.71g/cm3±0. P0 P<0. (P<0.0 568mgdL 5.68mg/dL±1 1653 16.53%±0 5995 59.95%± 152gcm3 1.52g/cm3±0 661mgdL 6.61mg/dL±0 1690 16.90%± 6030 60.30%± 171gcm3 1.71g/cm3±0 P<0 (P<0. 5.68mg/dL± 165 16.53%± 599 59.95% 152gcm 1.52g/cm3± 6.61mg/dL± 169 16.90% 603 60.30% 171gcm 1.71g/cm3± P< (P<0 5.68mg/dL 16.53% 59.95 1.52g/cm3 6.61mg/dL 16.90 60.30 1.71g/cm3 (P< 16.53 59.9 1.52g/cm 16.9 60.3 1.71g/cm 16.5 59. 16. 60.
15.
Particleboard Composite Made from Pinus and Eucalyptus Residues and Polystyrene Waste Partially Replacing the Castor Oil-Based Polyurethane as Binder OilBased Oil Based
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Rodrigues, Felipe R.
; Bispo, Rodrigo A.
; Cazell, Pedro Henrique
; Silva, Michael Jones
; Christoforo, André L.
; Silva, Sergio A. M.
.
In Brazil, native forests and replanted forests are poorly managed, and there is concern about reusing plastic residues as well. These two factors play a significant role in the impacts on the environment in the past decades. In this study, the influence of polystyrene (PS) waste partially replacing polyurethane (PUR) as a binder for wood particles (Pinus taeda L. and Eucalyptus saligna) was examined on the physicomechanical and thermal properties of homogeneous particleboards. For the production of particleboard composite, the moisture content of wood particles was set at 2%. A variety of physicomechanical characteristics were evaluated, including density, moisture content, swelling in thickness after 24 hours of immersion in water, rupture modulus (MOR) and elasticity modulus (MOE). Increasing PS relative to PUR decreased MOR and MOE properties in particleboard composite specimens. Thermal analysis shows that replacing PUR with PS in particleboard composite specimens has not adversely affected the thermal stability, and even less its thermal profile of specimens. ABNT NBR standards were exceeded by particleboard composite-based panels, but ANSI standards were met, indicating their potential application. As a result of this study, PS waste could be used as a binder for particleboards and composite materials manufactured from pinus and eucalyptus wood chips in place of PUR. Brazil managed well decades study (PS (PUR Pinus L saligna 2 2% evaluated density water (MOR MOE. . (MOE) stability compositebased based panels met application (MOE
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