ABSTRACT Objective: Abdominal ultrasonography is widely used to evaluate suspected cases of appendicitis. Objective descriptions of the direct and indirect signs of appendicitis result in varied assessments of its likelihood. This study introduces the Appendix Imaging Reporting and Data System (APENDIC-RADS) to standardize the reporting of appendix ultrasound findings. Methods: This single-center retrospective study included consecutive patients of all ages who underwent abdominal ultrasonography for the investigation of acute appendicitis. The primary outcome was histopathological confirmation of acute appendicitis post-surgery. The imaging findings were classified into five categories: APENDIC-RADS 0, where the appendix cannot be visualized; APENDIC-RADS 1, indicating a normal appendix; APENDIC-RADS 2, describing an appendix that is likely normal but only partially visualized; APENDIC-RADS 3, appendicitis cannot be ruled out due to uncertain features and APENDIC-RADS 4, acute appendicitis. Results: A total of 747 patients were assessed for suspected acute appendicitis using ultrasonography. Of the diagnosed patients, 52% were male, primarily exhibiting symptoms such as nausea and/or vomiting (60%), right iliac fossa pain (54%), and sudden decompression in the right iliac fossa (24%). Stratification into APENDIC-RADS categories revealed a significant variation in the incidence of acute appendicitis, with incidence rates of 4.5% for category 0 and 0.7%, 2.2%, 11.5%, and 93.5% for categories 1 to 4, respectively (p<0.001). The APENDIC-RADS showed excellent discriminative ability, evidenced by an area under the receiver operating characteristic curve of 0.950 (95%CI=0.899-1). Conclusion: APENDIC-RADS categorization demonstrated excellent performance in standardizing the ultrasound-determined probability of acute appendicitis. Its implementation could improve physician communication and standardization of patient management. likelihood APENDICRADS APENDIC RADS (APENDIC-RADS Methods singlecenter single center postsurgery. postsurgery post surgery. surgery post-surgery visualized 2 3 4 Results 74 52 male andor or 60%, 60 60% , (60%) 54%, 54 54% (54%) 24%. 24 24% . (24%) 45 5 4.5 07 7 0.7% 22 2.2% 115 11 11.5% 935 93 93.5 p<0.001. p0001 p p<0.001 001 (p<0.001) ability 0950 950 0.95 95%CI=0.8991. 95CI08991 CI 95%CI=0.899 95 899 (95%CI=0.899-1) Conclusion ultrasounddetermined determined management 6 (60% (54% (24% 4. 0.7 2.2 11.5 9 93. p000 p<0.00 00 (p<0.001 095 0.9 8991 95%CI=0.8991 95CI0899 95%CI=0.89 89 (95%CI=0.899-1 (60 (54 (24 0. 2. 11. p00 p<0.0 (p<0.00 09 95CI089 95%CI=0.8 8 (95%CI=0.899- (6 (5 (2 p0 p<0. (p<0.0 95CI08 95%CI=0. (95%CI=0.899 ( p<0 (p<0. 95CI0 95%CI=0 (95%CI=0.89 p< (p<0 95CI 95%CI= (95%CI=0.8 (p< 95%CI (95%CI=0. (p (95%CI=0 (95%CI= (95%CI

Abstract Dissolution tests evaluate the release of therapeutic agents in various dosage forms, acting as quality control tools to secure batch–batch equivalence and guides for formulation development and in vivo drug bioavailability prediction for pharmaceutical scientists. In this article, dissolution tests described in the Brazilian Pharmacopeia 6th ed. were systematically reviewed using the following descriptors: drug, dosage forms, apparatus, rotational speed, dissolution media, sampling time, quantitative procedure, and the value of Q . Test conditions were compared with those described in the United States Pharmacopeia (USP) dissolution database. In September, 2023, dissolution tests were required for 127 monographs, accounting for only 10% of those listed in the USP database. Paddles were used in 80 monographs (63.5%) at various rotation speeds. Basket apparatus was recommended for 47 products, including tablets, capsules, and gastro-resistant granules with variable speed ranges. The simulated gastric fluid was described in four monographs. Moreover, pH of the dissolution media for 29 products was adjusted in the physiological range of 2–7.5. Twenty-eight monographs are exclusively listed in the Brazilian Pharmacopeia. Among the 99 products listed in both compendiums, dissolution tests were only harmonized for 69 monographs. forms batchbatch batch scientists article th ed descriptors time procedure (USP database September 2023 12 10 8 63.5% 635 63 5 (63.5% speeds 4 tablets capsules gastroresistant gastro resistant ranges Moreover 2 275 7 2–7.5 Twentyeight Twenty eight 9 compendiums 6 202 1 63.5 (63.5 27 2–7. 20 63. (63. 2–7 (63 2– (6 (

RESUMO Introdução: A simulação médica emerge como uma metodologia crucial, permitindo que profissionais experimentem uma variedade de cenários, tanto de êxito quanto de falha, identificando fragilidades e condições inseguras, com o intuito de evitar danos aos pacientes. Assim, o objetivo desse estudo é descrever a produção de um modelo de simulação em apendicectomia videolaparoscópica “ex-vivo”. Metodologia: Foram utilizadas estruturas de cerâmica fria para moldar manualmente o modelo anatômico do apêndice, garantindo sua rigidez. Neste modelo, utilizou-se materiais para criar um molde flexível usando silicone acético. Feito o molde, o preencheu-se com borracha de polímero de estireno termomoldável, junto com corante, e fundindo em uma temperatura específica. Resultados: Esse processo resultou na confecção de uma peça que simula o apêndice, sendo resistente ao rasgo e suturável, replicando fielmente a estrutura e características de um órgão humano. O baixo peso dos materiais facilita o transporte, permitindo que sejam reproduzidos e utilizados em diversas situações, desde treinamentos em ambientes hospitalares até universidades. O modelo é aplicável em simulações didáticas com estudantes de medicina, residentes e cirurgiões. Sua facilidade de produção e baixo custo contribuem para que as práticas sejam repetíveis, garantindo um melhor desenvolvimento das habilidades cirúrgicas. Conclusão: O trabalho contribui para o avanço da simulação médica e destaca a importância de soluções inovadoras e colaborativas na melhoria da educação médica e na promoção da segurança do paciente. Introdução crucial cenários falha inseguras pacientes Assim exvivo. exvivo ex vivo . “ex-vivo” Metodologia apêndice rigidez utilizouse utilizou se acético preencheuse preencheu termomoldável corante específica Resultados suturável humano transporte situações universidades medicina cirurgiões repetíveis cirúrgicas Conclusão paciente “ex-vivo

ABSTRACT Introduction: Appendectomy is the standard treatment for appendicitis, with the laparoscopic technique offering benefits like lower infection rates and quicker recovery. However, residents often have their first practical experience with the procedure on real patients, increasing surgical risks. In this context, medical simulation emerges as a crucial methodology, allowing professionals to experience a variety of scenarios while preventing harm to patients. The objective of this study is to describe the production of an “ex-vivo” simulation model for laparoscopic appendectomy. Methodology: Cold ceramic structures were used to manually shape the anatomical model of the appendix, ensuring its rigidity. On this model, we poured materials to create a flexible mold using acetic silicone. Once the mold was made, we filled it with thermo-moldable styrene polymer rubber, along with dye, and fused it at a specific temperature. Results: This process resulted in the manufacture of a piece that simulates the appendix, being tear-resistant and suturable, faithfully replicating the structure and characteristics of a human organ. The low weight of the materials facilitates transport, allowing them to be reproduced and used in various situations, from training in hospital settings to universities. The model is applicable in didactic simulations with medical students, residents, and surgeons. Its ease of production and low cost contribute to the practices being repeatable, ensuring a better development of surgical skills. Conclusion: This work not only contributes to the advancement of medical simulation but also highlights the importance of innovative and collaborative solutions in improving medical education and promoting patient safety. Introduction appendicitis recovery However patients risks context methodology exvivo ex vivo “ex-vivo appendectomy Methodology appendix rigidity silicone made thermomoldable thermo moldable rubber dye temperature Results tearresistant tear resistant suturable organ transport situations universities students surgeons repeatable skills Conclusion safety

RESUMO A viticultura é a principal produção agrícola do Vale do São Francisco, Brasil; no entanto, os sistemas agrícolas de manejo dos solos requerem grandes volumes de fertilizantes que podem contribuir para as alterações climáticas. Portanto, foi relatado que o uso de rizobactérias promotoras de crescimento de plantas (RPCP) reduz ou substitui insumos vegetais. Este estudo teve como objetivo avaliar as bactérias da rizosfera de Vitis vinifera cultivada no Vale do São Francisco, em Pernambuco, Brasil, e caracterizar os mecanismos que promovem o crescimento das plantas. As RPCP com maior potencial biotecnológico foram identificadas por meio de sequenciamento. As bactérias foram isoladas em meio de cultura Agar Triptona de Soja (TSA) inoculado com 100 µL de uma diluição seriada. Os isolados obtidos foram caracterizados fenotipicamente e testados quanto à capacidade de solubilizar fosfato, promover fixação biológica de nitrogênio (FBN) e produzir ácido indol-3 acético (AIA), biofilmes e fatores antibióticos contra Xanthomonas campestris pv. vitícola. Um total de 423 bactérias foi obtido, das quais 99 apresentaram resultados positivos para pelo menos um dos mecanismos de promoção de crescimento, representando 6,85% para solubilizadores de fosfato, 0,74% para FBN, 5,7% para sintetizadores de AIA, 11,27% para produtores de biofilme e 4,01% para promoção de antibiose contra X. campestris pv. vitícola. Os isolados 3.19 e 31.14; 3.17 e 17.04; 5.35 e 5.42; e 5.37 identificados como Stenotrophomonas, Bacillus, Pseudomonas e Clostridium, respectivamente, apresentaram potencial biotecnológico para futuros experimentos para promover o crescimento de videiras. Francisco Brasil entanto climáticas Portanto (RPCP vegetais Pernambuco sequenciamento TSA (TSA 10 seriada fosfato FBN (FBN indol3 indol 3 indol- AIA , (AIA) pv vitícola 42 obtido 9 685 6 85 6,85 074 0 74 0,74 57 5 7 5,7 1127 11 27 11,27 401 4 01 4,01 X 319 19 3.1 31.14 3114 31 14 317 17 17.04 1704 04 535 35 5.3 5.42 542 537 37 Stenotrophomonas Bacillus Clostridium respectivamente videiras 1 (AIA 68 8 6,8 07 0,7 5, 112 2 11,2 40 4,0 3. 31.1 311 17.0 170 53 5. 5.4 54 6, 0, 11, 4, 31. 17.

ABSTRACT Viticulture is the main agricultural production in the São Francisco Valley, Brazil; however, farm soil management systems require large volumes of fertilizer that could contribute to climate change. Therefore, using plant growth-promoting rhizobacteria (PGPR) has been reported to reduce or replace plant input. This study aimed to evaluate the bacteria in the rhizosphere of Vitis vinifera cultivated in the São Francisco Valley in Pernambuco, Brazil, and to characterize the mechanisms that promote plant growth. The PGPR with the greatest biotechnological potential was identified using sequencing. The bacteria were isolated from tryptic soy agar (TSA) culture medium inoculated with 100 µL of a serial dilution. The isolates obtained were characterized phenotypically and tested for their ability to solubilize phosphate, promote biological nitrogen fixation (BNF), and produce indole-3 acetic acid (IAA), biofilms, and antibiotic factors against Xanthomonas campestris pv. viticola. A total of 423 bacteria were obtained, of which 99 presented positive results for at least one of the growth-promotion mechanisms, representing 6.85% for phosphate solubilizers, 0.74% for BNF, 5.7% for IAA synthesizers, 11.27% for biofilm producers, and 4.01% for promoting antibiosis against X. campestris pv. viticola. Isolates 3.19 and 31.14; 3.17 and 17.04; 5.35 and 5.42; and 5.37 identified as Stenotrophomonas, Bacillus, Pseudomonas, and Clostridium, respectively, presented a biotechnological potential for future experiments to promote vine growth. Brazil however change Therefore growthpromoting growth (PGPR input Pernambuco sequencing TSA (TSA 10 dilution BNF , (BNF) indole3 indole 3 indole- IAA, (IAA) biofilms pv viticola 42 9 growthpromotion promotion 685 6 85 6.85 solubilizers 074 0 74 0.74 57 5 7 5.7 synthesizers 1127 11 27 11.27 producers 401 4 01 4.01 X 319 19 3.1 31.14 3114 31 14 317 17 17.04 1704 04 535 35 5.3 5.42 542 537 37 Stenotrophomonas Bacillus Pseudomonas Clostridium respectively 1 (BNF (IAA 68 8 6.8 07 0.7 5. 112 2 11.2 40 4.0 3. 31.1 311 17.0 170 53 5.4 54 6. 0. 11. 4. 31. 17.

Abstract This study aimed to analyze the physical features and effect of hydroxyapatite (HAp) and Niobium/HAp CaNb2O6/Ca3(PO4)2 composite on critical size defect in rat calvaria. Biomaterials were characterized by X-ray diffraction (XRD), X-ray fluorescence (XRF), Particle size distribution (PSD) and Scanning Electron Microscopy (SEM). Critical size (8 mm) calvarial defects were created in Wistar rats (n = 24) distributed in three groups (90 days): Control group (CG): critical defect no treatment; HAp group: Hydroxyapatite; CNb/CPO group: CaNb2O6/Ca3(PO4)2 composite. After 90 days, calvaria samples were subjected to histological, and immunohistochemical reactions for osteopontin and osteocalcin, and Micro-CT analyses. XRD analysis confirmed the formation of a crystalline structure of CNb/CPO. XRF measurements confirmed the presence of Nb added in the sample CNb/CPO. Fracture toughness from CNb/CPO composite was 0.82 MPa.m1/2. Micro-CT showed increased trabecular number (p˂0.001), decreased porosities (%) (p˂0.001) and decreased trabecular separation (mm) (p˂0.001) in the CNb/CPO compared to CG group. In CNb/CPO group showed presence of new bone in which connective tissue differentiates to form or indicate a bone matrix score 3 (3-3.75, p˂0.05) and strong immunoexpression of osteopontin (p<0.05). The findings indicate that CNb/CPO showed resistance to the propagation of cracks and stimulated bone formation in calvaria defects in rats. (HAp NiobiumHAp Niobium CaNb2O6/Ca3PO42 CaNb2O6Ca3PO42 CaNbOCaPO CaNb2O6/Ca3 PO4 2 CaNb2O6 Ca3 CaNb O Ca PO CaNb2O6/Ca3(PO4) Xray X ray XRD, , (XRD) XRF, (XRF) PSD (PSD SEM. SEM . (SEM) 8 ( mm n 24 (9 days days) (CG) treatment Hydroxyapatite CNbCPO CNb CPO 9 histological osteocalcin MicroCT Micro CT analyses 082 0 82 0.8 MPam12 MPam MPa m1 m MPa.m1/2 p˂0.001, p0001 p p˂0.001 001 % (% (p˂0.001 (mm 33.75, 3375 3.75, 75 (3-3.75 p˂0.05 p005 05 p<0.05. p<0.05 (p<0.05) Ca3PO42 CaNb2O6/Ca3PO4 CaNb2O6Ca3PO4 CaNb2O6Ca3 CaNbOCa CaNb2O6/Ca CaNbO CaNb2O CaNb2O6/Ca3(PO4 (XRD (XRF (SEM (CG 08 0. MPam1 MPa.m1/ p000 p˂0.00 00 (p˂0.00 33 33.75 337 375 3.75 7 (3-3.7 p˂0.0 p00 p<0.0 (p<0.05 CaPO Ca3PO4 CaNb2O6/Ca3PO CaNb2O6Ca3PO CaNb2O6Ca CaNb2O6/Ca3(PO MPa.m1 (p˂0.0 33.7 37 3.7 (3-3. p˂0. p0 p<0. (p<0.0 Ca3PO MPa.m (p˂0. 33. 3. (3-3 p˂0 p<0 (p<0. (p˂0 (3- p˂ p< (p<0 (p˂ (3 (p< (p

Resumo Crajubar é a região formada pelos municípios Crato, Juazeiro do Norte e Barbalha situados ao sul do estado do Ceará. A expansão urbana desordenada com a retirada de parte significativa da vegetação, mudança da topografia local e aterro de corpos hídricos tem contribuído para a formação de ilhas de calor e consequentemente afetando negativamente a qualidade de vida da população local. Desta forma, o objetivo deste trabalho, foi realizar uma análise temporal da temperatura de superfície em função do uso e ocupação do solo, relacionando com a cobertura do solo por meio do índice de Vegetação por Diferença Normalizada (NDVI). Foi considerada uma série histórica dos anos de 2000, 2010 e 2020, utilizando imagens do Landsat 5, sensor TM nas bandas 3, 4 e 6 e Landsat 8, sensor OLI, nas bandas 4, 5 e 10 adquiridas da United States Geological Survey (USGS). Os resultados evidenciaram a formação de ilhas de calor nos bairros intensamente edificados e com classes de solos expostos, enquanto as ilhas de frescor compõem os corpos hídricos e a vegetação que os margeiam. A arborização da cidade com plantas perenifólias pode favorecer a redução da temperatura local principalmente nos meses de estiagem. Crato Ceará forma trabalho NDVI. NDVI . (NDVI) 2000 201 2020 3 8 OLI 1 USGS. USGS (USGS) expostos margeiam estiagem (NDVI 200 20 202 (USGS 2

Abstract Crajubar is a region formed by the counties of Crato, Juazeiro do Norte and Barbalha located in the south of the state of Ceará. Disorderly and acellerated urban expansion causes the removal of a significant part of the vegetation, alter the local topography and landfilling of rivers, lakes, dams contribute to the formation of heat islands and consequently affecting the quality of life of the local population. Therefore, the objective of this study was to carry out a temporal analysis of surface temperature as a function of land use and occupation, relating it to soil cover using the Normalized Difference Vegetation Index (NDVI). A historical series from the years 2000, 2010 and 2020 was considered, using images from Landsat 5, TM sensor in bands 3, 4 and 6 and Landsat 8, OLI sensor, in bands 4, 5 and 10 acquired from the United States Geological Survey (USGS). The city's afforestation with evergreen plants can help reduce local temperatures, especially during the dry months. Crato Ceará vegetation rivers lakes population Therefore occupation NDVI. NDVI . (NDVI) 2000 201 202 considered 3 8 1 USGS. USGS (USGS) citys city s temperatures months (NDVI 200 20 (USGS 2

Resumo Antecedentes O prognóstico da hemorragia subaracnoidea (HSA) permanece ruim. Vasoespasmo pode estar associado à inflamação. Razões neutrófilo-linfócito (NLR) e plaqueta-linfócito (PLR) têm sido estudadas como marcadores de inflamação e prognóstico. Objetivo Investigar NLR e PLR na admissão como preditores de vasoespasmo angiográfico e desfecho aos 6 meses. Métodos Este estudo de coorte incluiu pacientes consecutivos com HSA aneurismática de um centro terciário. Contagem de leucócitos, neutrófilos, linfócitos e plaquetas, proporção de neutrófilos para linfócitos e de plaquetas para linfócitos foram coletados como variáveis independentes. Ocorrência de vasoespasmo, escala de Rankin modificada, escala de desfecho de Glasgow e o escore de Hunt-Hess na admissão e 6 meses após a mesma foram registradas como variáveis dependentes. Modelos de regressão logística multivariável foram usados para ajustar potenciais fatores de confusão e avaliar valor prognóstico independente de NLR e PLR. Resultados Um total de 74,1% pacientes eram do sexo feminino, com idade média de 55,6 ± 12,4 anos. Na admissão, a pontuação média de Hunt-Hess foi de 2 (IQR 1) e a mediana de mFisher foi de 3 (IQR 1). Clipagem microcirúrgica foi o tratamento escolhido para 66,2% dos pacientes. A incidência de vasoespasmo angiográfico foi de 16,5%. Aos 6 meses, a escala de desfecho de Glasgow mediana era 4 (IQR 0,75) e a escala de Rankin modificada mediana era 3 (IQR 1,5). Vinte e um pacientes (15,1%) morreram. Os níveis de NLR e PLR não diferiram entre resultados funcionais favoráveis e desfavoráveis (mRS > 2 ou GOS < 4). Nenhuma variável foi significativamente associada ao vasoespasmo angiográfico. Conclusão Razão neutrófilo-linfócito e a PLR não apresentaram valor preditivo de desfecho funcional ou risco de vasoespasmo angiográfico. Mais pesquisas são necessárias neste campo. (HSA ruim neutrófilolinfócito neutrófilo linfócito (NLR plaquetalinfócito plaqueta (PLR terciário leucócitos independentes HuntHess Hunt Hess dependentes 741 74 1 74,1 feminino 556 55 55, 124 12 12, anos IQR 1. . 662 66 66,2 165 16 5 16,5% 0,75 075 0 75 1,5. 15 1,5 1,5) 15,1% 151 (15,1% morreram mRS 4. 4) campo 7 74, 66, 16,5 0,7 07 1, 15,1 (15,1 16, 0, 15, (15, (15 (1 (

Abstract Background Subarachnoid hemorrhage (SAH) prognosis remains poor. Vasospasm mechanism might be associated with inflammation. Neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) have been studied as inflammation markers and prognostic predictors. Objective We aimed to investigate NLR and PLR in admission as predictors of angiographic vasospasm and functional outcome at 6 months. Methods This cohort study included consecutive aneurysmal SAH patients admitted to a tertiary center. Complete blood count was recorded at admission before treatment. White blood cell count, neutrophil count, lymphocyte count, platelet count, NLR, and PLR were collected as independent variables. Vasospasm occurrence-modified Rankin scale (mRS), Glasgow outcome scale (GOS), and Hunt-Hess score at admission and at 6 months were recorded as dependent variables. Multivariable logistic regression models were used to adjust for potential confounding and to assess the independent prognostic value of NLR and PLR at admission. Results A total of 74.1% of the patients were female, with mean age of 55.6 ± 12.4 years. At admission, the median Hunt-Hess score was 2 (interquartile range [IQR] 1), and the median mFisher was 3 (IQR 1). Microsurgical clipping was the treatment for 66.2% of the patients. Angiographic vasospasm incidence was 16.5%. At 6 months, the median GOS was 4 (IQR 0.75), and the median mRS was 3 (IQR 1.5). Twenty-one patients (15.1%) died. Neutrophil-to-lymphocyte ratio and PLR levels did not differ between favorable and unfavorable (mRS > 2 or GOS < 4) functional outcomes. No variables were significantly associated with angiographic vasospasm. Conclusion Admission NLR and PLR presented no value for prediction of functional outcome or angiographic vasospasm risk. Further research is needed in this field. (SAH poor Neutrophiltolymphocyte Neutrophil (NLR platelettolymphocyte (PLR center occurrencemodified occurrence modified mRS, , (mRS) GOS, (GOS) HuntHess Hunt Hess 741 74 1 74.1 female 556 55 55. 124 12 12. years interquartile IQR [IQR 1, 1) 1. . 662 66 66.2 165 16 5 16.5% 0.75, 075 0.75 0 75 0.75) 1.5. 15 1.5 1.5) Twentyone Twenty one 15.1% 151 (15.1% died outcomes risk field (GOS 7 74. 66. 16.5 07 0.7 15.1 (15.1 16. 0. 15. (15. (15 (1 (

Abstract Objective: To assess the predictive value of selected growth phenotypes for neonatal morbidity and mortality in preterm infants < 30 weeks and to compare them with INTERGROWTH-21st (IG21). Methods: Retrospective analysis of data from the Brazilian Neonatal Research Network (BNRN) database for very low birth weight (VLBW) at 20 public tertiary-care university hospitals. Outcome: the composite neonatal morbidity and mortality (CNMM) consisted of in-hospital death, oxygen use at 36 weeks, intraventricular hemorrhage grade 3 or 4, and Bell stage 2 or 3 necrotizing enterocolitis. Selected growth phenotypes: small-for-gestational-age (SGA) defined as being < 3rd (SGA3) or 10th (SGA10) percentiles of BW, and large-for-gestational-age (LGA) as being > 97th percentile of BW. Stunting as being < 3rd percentile of the length and wasting as being < 3rd percentile of BMI. Single and multiple log-binomial regression models were fitted to estimate the relative risks of CNMM, comparing them to IG21. Results: 4,072 infants were included. The adjusted relative risks of CNMM associated with selected growth phenotypes were (BNRN/IG21): 1.45 (0.92–2.31)/1.60 (1.27–2.02) for SGA; 0.90 (0.55–1.47)/1.05 (0.55–1.99) for LGA; 1.65 (1.08–2.51)/1.58 (1.28–1.96) for stunting; and 1.48 (1.02–2.17) for wasting. Agreement between the two references was variable. The growth phenotypes had good specificity (>95%) and positive predictive value (70-90%), with poor sensitivity and low negative predictive value. Conclusion: The BNRN phenotypes at birth differed markedly from the IG21 standard and showed poor accuracy in predicting adverse neonatal outcomes. Objective INTERGROWTH21st INTERGROWTHst INTERGROWTH 21st st IG . (IG21) Methods (BNRN VLBW (VLBW tertiarycare tertiary care hospitals Outcome (CNMM inhospital hospital death 4 enterocolitis smallforgestationalage small gestational age SGA (SGA rd SGA3 (SGA3 th SGA10 (SGA10 BW largeforgestationalage large LGA (LGA BMI logbinomial log binomial Results 4072 072 4,07 included BNRN/IG21 BNRNIG21 BNRNIG (BNRN/IG21) 145 1 45 1.4 0.92–2.31/1.60 092231160 0.92–2.31 /1.60 0 92 31 60 (0.92–2.31)/1.6 1.27–2.02 127202 27 02 (1.27–2.02 090 90 0.9 0.55–1.47/1.05 055147105 0.55–1.47 /1.05 55 47 05 (0.55–1.47)/1.0 0.55–1.99 055199 99 (0.55–1.99 165 65 1.6 1.08–2.51/1.58 108251158 1.08–2.51 /1.58 08 51 58 (1.08–2.51)/1.5 1.28–1.96 128196 28 96 (1.28–1.96 stunting 148 48 1.02–2.17 102217 17 (1.02–2.17 variable >95% 95 (>95% 7090%, 7090 70 90% , (70-90%) Conclusion IG2 outcomes (IG21 SGA1 (SGA1 407 07 4,0 BNRN/IG2 BNRNIG2 (BNRN/IG21 14 1. 0.92–2.31/1.6 09223116 092231 0.92–2.3 160 /1.6 9 6 (0.92–2.31)/1. 1.27–2.0 12720 (1.27–2.0 09 0. 0.55–1.47/1.0 05514710 055147 0.55–1.4 105 /1.0 5 (0.55–1.47)/1. 0.55–1.9 05519 (0.55–1.9 16 1.08–2.51/1.5 10825115 108251 1.08–2.5 158 /1.5 (1.08–2.51)/1. 1.28–1.9 12819 (1.28–1.9 1.02–2.1 10221 (1.02–2.1 >95 (>95 7090% 709 7 (70-90% (IG2 40 BNRN/IG (BNRN/IG2 0.92–2.31/1. 0922311 09223 0.92–2. /1. (0.92–2.31)/1 1.27–2. 1272 (1.27–2. 0.55–1.47/1. 0551471 05514 0.55–1. 10 (0.55–1.47)/1 0551 (0.55–1. 1.08–2.51/1. 1082511 10825 1.08–2. 15 (1.08–2.51)/1 1.28–1. 1281 (1.28–1. 1.02–2. 1022 (1.02–2. >9 (>9 (70-90 (IG (BNRN/IG 0.92–2.31/1 0922 0.92–2 /1 (0.92–2.31)/ 1.27–2 127 (1.27–2 0.55–1.47/1 0.55–1 (0.55–1.47)/ 055 (0.55–1 1.08–2.51/1 1082 1.08–2 (1.08–2.51)/ 1.28–1 128 (1.28–1 1.02–2 102 (1.02–2 (> (70-9 0.92–2.31/ 092 0.92– / (0.92–2.31) 1.27– 12 (1.27– 0.55–1.47/ 0.55– (0.55–1.47) (0.55– 1.08–2.51/ 108 1.08– (1.08–2.51) 1.28– (1.28– 1.02– (1.02– ( (70- 0.92 (0.92–2.31 1.27 (1.27 0.55 (0.55–1.47 (0.55 1.08 (1.08–2.51 1.28 (1.28 1.02 (1.02 (70 (0.92–2.3 1.2 (1.2 0.5 (0.55–1.4 (0.5 1.0 (1.08–2.5 (1.0 (7 (0.92–2. (1. (0. (1.08–2. (0.92–2 (1 (0 (1.08–2 (0.92– (1.08– (0.92 (1.08 (0.9

ABSTRACT Brazilian semi-arid tropical is responsible for the production and export of fine table grapes, but the demand for phosphate fertilizers and other agricultural inputs increases the cost of production and threatens local water bodies by leaching of phosphorus (P). The objectives of this study were to quantify the organic and inorganic fractions of P and to determine chemical and microbiological attributes related to its mineralization in soils cultivated with vines in São Francisco Valley. It was hypothesized that P fractions and microbial activity would increase in areas fertilized with phosphate. Soil samples from vines were collected in cultivated areas and in fallow systems. P fractionation, macro-elements analysis, microbial biomass phosphorus (Pmic), and phosphatase enzyme activity were determined and correlated with each other and with the total proportion of synthesized glomalin. In both treatments, the inorganic P fractions exceeded the organic ones, and the total phosphorus available in those soils was associated with the most recalcitrant fractions, reducing risks of leaching of this element and contamination of water bodies. Pmic was higher in the winery areas due to the presence of phosphate fertilizers and organic matter, hence the activity of alkaline phosphatase enzymes and the production of total glomalin increased respectively in three and four evaluated areas, indicating that P-mineralized bacteria and arbuscular mycorrhizal fungi have been positively affected by the current agricultural system. semiarid semi arid grapes P. . (P) Valley systems fractionation macroelements macro elements analysis Pmic, , (Pmic) treatments ones matter Pmineralized mineralized system (P (Pmic

RESUMO Os testes de consolidação fornecem informações importantes sobre a compactabilidade do solo. No entanto, eles requerem equipamentos especiais, disponíveis em poucos laboratórios no Brasil. Esta região é uma proeminente produtora de alimentos e fibras em solos coesos. Esses solos apresentam problemas frequentes de compactação. Como a mandioca é um dos principais produtos, suas águas residuais (manipueira) são frequentemente utilizadas para adubação. Esse uso pode afetar a compactação devido à sua influência na dispersão-floculação de partículas. A compressão uniaxial e modelos matemáticos são as principais formas de medir ou estimar a compactação do solo. Aqui, a compressão foi medida com um teste alternativo de centrifugação usando amostras deformadas, buscando um método alternativo simples e rápido. Foram usadas cargas específicas (cilindros de aço inoxidável: massa) e força G crescente (peso) em centrífuga, para simular a pressão nas amostras usadas na maioria dos aparelhos de consolidação. Os solos, as camadas de amostragem e a presença de água residuária (manipueira) também foram comparados, pois podem afetar os atributos relacionados à compactabilidade. Foram utilizadas amostras de Argissolo Cinzento Coeso e Latossolo Amarelo Distrocoeso (profundidades de 0-0,20 m e 0,20-0,40 m), ambos provenientes dos Tabuleiros costeiros do estado da Bahia, Brasil. A água residual afetou o teor de argila dispersa em água, a estabilidade dos agregados, o pH, o ∆pH, a floculação e o teor de carbono orgânico, influenciando a compactabilidade, mas não a umidade e a densidade máxima medidas pelo teste de Proctor. Embora a centrifugação tenha proporcionado menor densidade do que o teste de Proctor, os resultados foram comparáveis àqueles estimados pelos modelos matemáticos. Este método também forneceu informações sobre a densidade, próxima aos valores que limitam o crescimento da raiz, e o teor de umidade que leva a essa densidade limitante. solo entanto especiais Brasil coesos produtos manipueira (manipueira adubação dispersãofloculação dispersão partículas Aqui deformadas rápido cilindros inoxidável massa peso (peso centrífuga comparados profundidades 00,20 0020 0 0,20 20 0-0,2 0,200,40 020040 0,40 40 0,20-0,4 m, , m) Bahia agregados pH ∆pH orgânico Proctor raiz limitante 00 00,2 002 020 0,2 2 0-0, 200 0,200,4 02004 040 0,4 4 0,20-0, 00, 02 0, 0-0 0,200, 0200 04 0,20-0 0- 0,200 0,20-

ABSTRACT Consolidation tests give important insights into soil compactibility. However, it requires equipment that is not always available. The costal tablelands in Northeastern Brazil have extensive areas of cohesive soils where compaction is an expressive problem. This region is a prominent producer of food and fibers with cassava as one of the leading products. Part of the cassava is used for flour production, generating wastewater, which is often applied as organic fertilizer to the soil. This can affect the compaction properties of soil due to its dispersing-flocculating characteristics. Uniaxial-compression tests and mathematical models are the primary methods to measure or estimate soil compaction. This study measured soil compression with a centrifuge, seeking a simple-quick method. We used specific loads (stainless-steel cylinders: mass) and increasing G-force (weight) to simulate the pressure on the samples used in most consolidation apparatus. Soils, sampling layers, and the presence of cassava wastewater (manipueira) were also compared since they may affect compactibility-related attributes. Samples of a Gray Cohesive Argisol and Dystrocohesive Yellow Latosol (depths of 0-0.20 m and 0.20-0.40 m) from the Tablelands of the state of Bahia, Brazil, were used. Wastewater affected water-dispersible clay, aggregate stability, pH, ∆pH, flocculation, and organic carbon, thus influencing compactibility but not the moisture and maximum density measured by the Proctor test. Centrifugation caused lower density than the Proctor test. The results were close to those estimated by the mathematical models, thus considered a promising alternative to estimating consolidation. This method also provides insight into the root growth-limiting density and the moisture content that leads to it. However available problem products production dispersingflocculating dispersing flocculating characteristics Uniaxialcompression Uniaxial centrifuge simplequick simple quick stainlesssteel stainless steel cylinders mass Gforce G force weight (weight apparatus Soils layers manipueira (manipueira compactibilityrelated related attributes depths 00.20 0020 0 0.20 20 0-0.2 0.200.40 020040 0.40 40 0.20-0.4 Bahia waterdispersible water dispersible clay stability pH ∆pH flocculation carbon test growthlimiting growth limiting 00 00.2 002 020 0.2 2 0-0. 200 0.200.4 02004 040 0.4 4 0.20-0. 00. 02 0. 0-0 0.200. 0200 04 0.20-0 0- 0.200 0.20-

ABSTRACT. Cohesive Oxisols are widely used for cultivating eucalyptus in the Coastal Tablelands of the northeastern region of Brazil. However, mechanization and plant cultivation in these soils are difficult because of their cohesive layers. Therefore, the objective of this study was to identify better combinations between tillage systems and types of fertilization to improve the physical attributes of cohesive soil, with the aim of improving eucalyptus growth. The experimental design was completely randomized in a 4 × 2 factorial scheme (soil preparation × fertilization). The tillage systems tested were: i) conventional tillage (CT) - one plowing combined with two harrowings, ii) minimum tillage (MT) - subsoiling down until 0.57 m depths in the planting line, iii) no-tillage type 1 (NT1) - planting in 0.3 m-deep pits, and iv) no-tillage type 2 (NT2) - planting in 0.6 m-deep pits. The types of fertilization tested were mineral (MF) and organic fertilization (OF). The diameter of the soil aggregates was reduced after being subjected to any combination of cohesive soils. Furthermore, OF provided the best levels of plant-available water, attenuating the adverse conditions of the cohesive layer. MT, NT1, and NT2 improved the soil physical attributes when compared to CT. Therefore, the combination of either NT1 or NT2 with OF, followed by the combination of either NT1 or NT2 with MF, was determined to be the best way to cultivate eucalyptus on cohesive soils. ABSTRACT Brazil However layers Therefore growth fertilization. . fertilization) i CT (CT harrowings ii MT (MT 057 0 57 0.5 line iii notillage no NT (NT1 03 3 0. mdeep deep pits iv (NT2 06 6 MF (MF OF. (OF) Furthermore plantavailable available water layer 05 5 (NT (OF

ABSTRACT Evaluating the effects of N and K supply on grapevines ( Vitis vinifera L.) and the techniques for nutritional diagnosis is of great importance for fertigation management of this crop. This study evaluated the effects of N and K fertigation on the soluble concentrations of NO 3 – and K + in the petiole sap and on the leaf chlorophyll index in drip irrigated ‘Syrah’ grapevine (from 17 June 2013 to 25 Nov 2014). The treatments consisted of five levels of N (0, 15, 30, 60 and 120 kg ha –1 ) and K 2 O (0, 15, 30, 60 and 120 kg ha –1 ), combined in an incomplete 5 2 factorial scheme in 13 combinations and arranged in randomized blocks with four replications. We determined NO 3 – and K + concentrations in petiole sap, leaf chlorophyll index, grapevine cluster mass and number per plant, mean grapevine cluster mass, and phenolic composition. High NO 3 – concentrations contributed positively to grapevine yield; however, increased K+ concentrations caused a negative response of sap. For 120 kg N ha –1 rate, NO 3 – in the sap and chlorophyll index showed higher values at the flowering stage, while high values for K + were observed during the grape-ripening stage.

RESUMO Objetivos: Avaliar a reformatação tomográfica sagital do esterno por meio de parâmetros radiográficos inéditos (índices e ângulos), comparando-os entre os diferentes tipos de pectus e controles. Métodos: 44 pacientes com deformidades pectus e controles foram submetidos à TC do tórax para análise. Os tipos de pectus foram classificados em: pectus carinatum inferior (PCI), superior (PCS) e lateral (PCL), e pectus excavatum amplo (PEA) e localizado (PEL). Foram criados e mensurados os seguintes parâmetros tomográficos: (1) índice coluna-manúbrio-esterno (CME); (2) índice coluna-esterno (CE); (3) ângulo manúbrio-esternal (AME); (4) ângulo inferior do manúbrio (AIM); e (5) ângulo inferior do esterno (AIE). Foi realizada análise estatística entre os grupos pectus e controle, e entre os diferentes tipos de pectus. Resultados: Houve diferença significativa entre: a) pectus excavatum e pectus carinatum quando analisados os índices CME, CE, AIM e AIE; b) PEL e grupo controle para CME e AIE; c) PCL e PEL, e PCL e PEA para o CME; d) PEA e PCL para CE; e) PCI e PEL, e PCI e PEA para AIE; f) PCS e PEL, e PCS e PEA para AIM. Conclusões: Os índices e ângulos radiográficos criados forneceram parâmetros de diferenciação entre pacientes com diferentes tipos de pectus, e entre estes e controles. Nível de Evidência II, Estudos Prognósticos.

ABSTRACT Objective: To evaluate the sagittal tomographic reformatting of the sternum using unpublished radiographic parameters (indexes and angles), comparing them between the different types of pectus, and controls. Methods: 44 patients with pectus deformities and controls underwent chest CT for analysis. The types of pectus were classified into: inferior pectus carinatum (IPC), superior (SPC) and lateral (LPC), and broad (BPE) and localized pectus excavatum (LPE). The following tomographic parameters were created and measured: (1) spine-manubrium-sternum index (SMS); (2) column-sternum index (CSI); (3) manubrium-sternal angle (MSA); (4) inferior manubrium angle (IMA); and (5) inferior sternum angle (ISA). Statistical analysis was performed between the pectus and control groups, and between the different types of pectus. Results: There was a significant difference between: a) pectus excavatum and pectus carinatum when analyzing the SMS, CSI, MSA and ISA indexes. b) LPE and control group for SMS and ISA. c) LPC and LPE, and LPC and BPE for SMS; d) BPE and LPC for CSI; e) IPC and LPE, and IPC and BPE for ISA; f) SPC and LPE, and SPC and BPE for IMA. Conclusion: The radiographic indexes and angles created provided differentiation parameters between patients with different types of pectus, and between these and controls. Level of Evidence II, Prognostic Studies.

Abstract Microbialites are the most abundant life evidence in Precambrian sedimentary rocks. They are produced by microbial interaction activity and sedimentary processes reflecting paleoenvironmental conditions. The Ediacaran-Cambrian carbonate and siliciclastic successions in the Southern Amazon Craton in Central Brazil, provide a key opportunity to understand how the metazoan life coexisted with the microbial communities. The spatial and temporal distribution of microbialites as well as morphological and paleoenvironmental changes have been assessed, reinterpreting previous works and including new data from the Araras-Alto Paraguai and Corumbá basins. The deposition was controlled by subsidence and sea-level changes that affected these basins, considered extensions of epicontinental seas during the Gondwana assembly. The stromatolites are restricted to coastal deposits and experienced thriving flourishment intervals after the Marinoan Glaciation (635 Ma). Post-glacial transgression was marked by microbial colonization in shallow platforms represented by stratiform and giant domical stromatolites in the Araras-Alto Paraguai Basin. The continuity of the transgression generated a moderately deep aragonite sea at about 622 Ma. A progressive sea-level fall caused the implantation of coastal environments under greenhouse conditions with tidal flat and sabkha settings colonized by centimetric-scale stromatolites. The sea retreat was accompanied by progressive uplift, causing a moderate inversion of the basin and erosion of the succession until ~560 Ma with the deposition of the last preserved tidal flat deposits with the occurrence of thrombolites. The subsiding Corumbá Basin was the site of microbially-induced deposition of carbonates in a shallow platform connected to an offshore setting with the proliferation of metazoan straddling the Ediacaran-Cambrian boundary. Microbial communities were restricted to lagoon deposits during the Lower Cambrian transgression in the Araras-Alto Paraguai Basin and the last phase refers to the sea retreat towards southeast, developing a fluvial system connected with the arid and evaporitic tidal flats colonized by microbialites that lasted until the upper Cambrian. Except for the post-glacial stromatolites, the columnar and domal microbialite indicate that the coastal settings dominated the Ediacaran-Cambrian transition. The preservation of microbialites in the post-glacial intervals can be associated with the Mg-Ca-CO3 oversaturation in dolomitic platforms. The rapid calcification and ability to resist the dissolution and replacement have increased the stromatolites’ preservation potential reported here, where its well-preserved occurrence in tidal flats and sabkha occurs due to intense early diagenetic silicification. The change from carbonate accumulation to siliciclastic-rich environments contributed to the demise of microbially-induced strata. In general, the scarce coexistence between coastal stromatolite and metazoan-bearing marine deposits makes it challenging to establish a competitive relationship between these organisms, as previously postulated.

RESUMO Objetivo: Avaliar a reformatação sagital tomográfica do esterno por meio da análise de uma classificação do tipo de curvatura do corpo esternal nos diferentes tipos de pectus, comparando-os entre si e com indivíduos normais. Métodos: 50 controles e 167 pacientes submetidos à TC do tórax para análise da reconstrução sagital no plano mediano, sendo 89 com pectus carinatum (idade média, 12 ± 10 anos) e 78 com pectus excavatum (idade média, 14 ± 10 years). Os tipos clínicos de pectus foram classificados em: pectus carinatum superior, inferior e lateral, e pectus excavatum amplo ou localizado. Foram definidos os seguintes tipos de padrões esternais: curvo gradativo vertical; curvo gradativo posterior; curvo gradativo anterior; curvo terço proximal; curvo terço médio; curvo terço distal; retilíneo anterior; retilíneo vertical; e retilíneo posterior. Foi realizada análise estatística entre o grupo pectus e controle, e entre diferentes tipos de pectus. Resultados: Observamos pacientes com deformidades torácicas diferentes, mas com esternos com padrão de curvatura semelhante. Alguns tipos de curvatura esternal são significativamente mais frequentes em alguns tipos de pectus (p < 0,05). Em controles prevaleceram os tipos curvo gradativo vertical e retilíneo anterior (p < 0,05). Conclusão: Alguns tipos de curvatura esternal são mais frequentes que outras em determinados tipos de pectus e controles. Nível de Evidência II, Estudos prognósticos - investigação do efeito de característica de um paciente sobre o desfecho da doença.

ABSTRACT Objective: To analyze reformatted sagittal sternal tomography images and classify sternal body curvature types, and compare different types of pectus populations with one another and with normal individuals. Methods: In total, 50 controls and 167 pectus patients were selected for chest CT to analyze the median sagittal plane, of whom 89 had pectus carinatum (mean age, 12 ± 10 years) and 78 pectus excavatum (mean age, 14 ± 10 years). Clinical types of pectus were classified as inferior, superior, or lateral pectus carinatum, and localized or broad pectus excavatum. The following types of sternal patterns were defined: gradual vertical curve, gradual posterior curve, gradual anterior curve, proximal third curve, middle third curve, distal third curve, anterior rectilinear, vertical rectilinear, and posterior rectilinear. Statistical analyses were performed to compare the different types of pectus with one another and with the control group. Results: Patients with different thoracic deformities, but with similar sternal curvature patterns, were observed. Some types of sternal curvature were significantly more frequent in certain types of pectus (p < 0,05). The gradual vertical curve and anterior rectilinear types prevailed in controls (p < 0,05). Conclusion: Some sternal curvature patterns were more frequent than the others in certain types of pectus and the controls. Level of Evidence II, Prognostic studies - investigating the effect of a patient characteristic on the outcome of disease.