RESUMO O parvovírus B19 (B19V) pode ser transmitido por via respiratória, verticalmente - da mãe para o feto - e via transfusão de sangue e transplante de órgãos. A infecção por transfusão de sangue ou hemoderivados ocorre devido à resistência do B19V aos métodos de inativação viral. Nosso estudo avaliou a presença do ácido desoxirribonucleico (DNA) B19V e a prevalência de imunoglobulina da classe G (IgG) anti-B19V em mulheres em idade fértil, doadoras de sangue do Distrito Federal, Brasil. Nossos resultados demonstraram a ausência de DNA de B19V nesses doadores. No entanto, foi observada a soroprevalência de IgG anti-B19V em 60,7% dessa população. Este estudo fornece dados importantes da circulação do B19V no Centro-Oeste do Brasil.

ABSTRACT Parvovirus B19 (B19V) can be transmitted by the respiratory route, vertically - from the mother to the fetus - and via blood transfusion or organ transplantation. Infection by transfusion of blood or blood products occurs due to the resistance of B19V to viral inactivation methods. Our study evaluated the presence of B19V deoxyribonucleic acid (DNA) and the prevalence of anti-B19V class G immunoglobulin (IgG) in women of childbearing age blood donors of the Federal District, Brazil. Our results demonstrated the absence of B19V DNA in these blood donors. However, the seroprevalence for anti-B19V IgG was observed in 60.7% of this population. This study provides important data of B19V circulation in the Center-West of Brazil.

RESUMO O lúpus eritematoso sistêmico (LES) é considerado uma doença autoimune devido à atuação de autoanticorpos, que ocasionam inflamações crônicas em diversos tecidos corporais. Considerando o envolvimento do fator de crescimento vascular endotelial (VEGF) no desenvolvimento da inflamação, este trabalho objetivou avaliar a frequência do polimorfismo na posição -2549 (Ins/Del 18pb, rs35569394) em pacientes com LES, comparando-os com indivíduos saudáveis. Não foram encontradas diferenças estatísticas ao comparar as frequências alélicas e genotípicas entre pacientes e controles, sugerindo que não há associação entre o desenvolvimento de LES e o polimorfismo estudado.

ABSTRACT Systemic lupus erythematosus (SLE) is considered an autoimmune disease characterized by the action of autoantibodies, which cause chronic inflammation in various tissues of the body. Considering the vascular endothelial growth factor (VEGF) participation in the development of inflammation, this study aimed to evaluate the frequency of the polymorphism at the -2549 position (Ins/Del 18pb, rs35569394) in patients with SLE and in healthy individuals. No statistical differences were found when comparing the allele and genotype frequencies between patients and controls, suggesting that there is no association between the studied polymorphism and the development of SLE.

RESUMO O objetivo foi analisar a estrutura da paisagem florestal entre duas Unidades de Conservação por meio de índices de ecologia da paisagem. O mapeamento foi obtido por digitalização de aerofoto, utilizando-se técnicas de fotointerpretação na escala 1:2500. No cálculo dos índices, foi utilizada Patch Analyst (ArcGis 9.3). Os fragmentos foram divididos em classes (muito pequeno: <5 ha; pequenos: >5 e <10 ha; médios: >10 e <100 ha; grandes: >100 ha). Foram calculados os índices de área, densidade, tamanho, forma, proximidade, área central (obtidos para diferentes simulações de efeito de borda: 40, 80, 100, 140 m). Encontraram-se 2.652 fragmentos, representando 42,6% de cobertura florestal. Os fragmentos muito pequenos dominam (2.201), seguidos pelos médios (216), pequenos (177) e grandes (58). Houve diferenciações em relação às classes, demonstrando que os maiores apresentam métricas que indicam boa conservação. As classes média e grande indicam a necessidade de criação de políticas públicas que incentivem a formação de corredores ecológicos entre ambas as áreas protegidas. aerofoto utilizandose utilizando se 12500. 12500 1 2500. 2500 1:2500 ArcGis 9.3. 93 9.3 . 9 3 9.3) pequeno 5 < ha > 10 <1 >1 100 ha. ha) densidade tamanho forma proximidade obtidos borda 40 80 14 m. m m) Encontraramse Encontraram 2652 2 652 2.65 426 42 6 42,6 2.201, 2201 2.201 , 201 (2.201) 216, 216 (216) 177 (177 58. 58 (58) conservação protegidas 1250 250 1:250 9. 4 8 265 65 2.6 42, 220 2.20 20 (2.201 21 (216 17 (17 (58 125 25 1:25 26 2. 22 2.2 (2.20 (21 (1 (5 12 1:2 (2.2 (2 ( 1: (2.

ABSTRACT The objective of this work was to analyze the structure of forest landscape, between two conservation areas, through indices of landscape ecology. Mapping was obtained by scanning aerial photographs using photo-interpretation techniques at the scale of 1:2500. Patch Analyst (ArcGis 9.3) was used for the indices calculation. The fragments were divided in the following classes: very small (<5 ha); small (>5 and <10 ha); medium (>10 and <100 ha); and large (>100 ha). Indices of area, density, size, shape, proximity and central area were calculated; being obtained for different simulations of edge effect (40, 80, 100, 140 m). 2,652 fragments were found, representing 42.6% of forest cover. The very small fragments were predominant (2,201), followed by medium (216), small (177), and large fragments (58). There were differences in relation to classes, which demonstrated that the greatest present metrics showed good conservation. The middle and high classes indicated that public policies which encourage the creation of ecological corridors between the two protected areas should be generated. ecology photointerpretation photo interpretation 12500. 12500 1 2500. 2500 1:2500 ArcGis 9.3 93 9 3 calculation <5 5 (< ha ha) >5 (> 10 <1 >10 (>1 100 >100 ha. . density size shape calculated 40, 40 (40 80 14 m. m m) 2652 2 652 2,65 found 426 42 6 42.6 cover 2,201, 2201 2,201 , 201 (2,201) 216, 216 (216) 177, 177 (177) 58. 58 (58) generated 1250 250 1:250 9. < ( > >1 4 (4 8 265 65 2,6 42. 220 2,20 20 (2,201 21 (216 17 (177 (58 125 25 1:25 26 2, 22 2,2 (2,20 (21 (17 (5 12 1:2 (2,2 (2 (1 1: (2,

In spite of the taxonomy of the Aspergillus species of the Nigri Section being regarded as troublesome, a number of methods have been proposed to aid in the classification of this Section. This work aimed to distinguish Aspergillus species of the Nigri Section from foods, grains and caves on the basis in Polyphasic Taxonomy by utilizing morphologic and physiologic characters, and sequencing of ß-tubulin and calmodulin genes. The morphologic identification proved useful for some species, such as A. carbonarius and Aspergillus sp UFLA DCA 01, despite not having been totally effective in elucidating species related to A. niger. The isolation of the species of the Nigri Section on Creatine Sucrose Agar (CREA) enabled to distinguish the Aspergillus sp species, which was characterized by the lack of sporulation and by the production of sclerotia. Scanning Electron microscopy (SEM) allowed distinguishing the species into two distinct groups. The production of Ochratoxin A (OTA) was only found in the A. carbonarius and A. niger species. The sequencing of β-tubulin gene was efficient in differing most of the Aspergillus species from the Nigri Section with the exception of Aspergillus UFLA DCA 01, which could not be distinguished from A. costaricaensis. This species is morphologically similar to A. costaricaencis for its low sporulation capacity and high sclerotia production, but it differs morphologically from A. costaricaensis for its conidial ornamentation and size of vesicles. Equally, based on partial calmodulin gene sequence data Aspergillus UFLA DCA 01 differs from A. costaricaensis.