Resumo A presente revisão integrativa tem por objetivo identificar os arranjos de cuidado em saúde mental que foram implementados no enfrentamento à pandemia de COVID-19. Realizou-se busca em três bases de dados (SciELO, PubMed e LILACS), em português, inglês e espanhol, com os descritores “SAÚDE MENTAL” or “SALUD MENTAL” or “MENTAL HEALTH” AND “COVID-19”, no período de 2020 a 2021. Foram encontrados 3.451 artigos, sendo 43 selecionados para análise. Em relação ao cuidado em saúde mental, os principais arranjos identificados foram os digitais, de natureza pública, desenvolvidos na esfera municipal e com integração com a rede de saúde. Os modelos de cuidado em saúde mental para o enfrentamento da pandemia são discutidos a partir dos tipos de arranjo produzidos nesse contexto sanitário emergencial e crítico. Apresenta-se, ainda, um recorte da realidade encontrada no Sistema Único de Saúde (SUS), reiterando sua resiliência. Concluiu-se que os arranjos digitais foram os mais usados e que há necessidade de investigar a acessibilidade deste modelo para populações com maior vulnerabilidade social. Reafirma-se a importância do SUS para o enfrentamento da COVID-19 e no acesso a informações de saúde. COVID19. COVID19 COVID 19. 19 Realizouse Realizou se SciELO, SciELO (SciELO LILACS, LILACS , LILACS) português espanhol SAÚDE MENTAL SALUD HEALTH COVID19, “COVID-19” 202 2021 3451 3 451 3.45 artigos 4 análise pública crítico Apresentase, Apresentase Apresenta se, Apresenta-se ainda SUS, (SUS) resiliência Concluiuse Concluiu social Reafirmase Reafirma COVID-1 COVID1 1 “COVID-19 20 345 45 3.4 (SUS COVID- “COVID-1 2 34 3. “COVID- “COVID

Abstract This integrative review aims to identify the mental health care measures that were produced during the COVID-19 pandemic. This research was conducted on three databases (SciELO, PubMed, and LILACS) with the following descriptors in Portuguese, English, and Spanish: “SAÚDE MENTAL” or “SALUD MENTAL” or “MENTAL HEALTH” AND “COVID-19” from 2020 to 2021. In total, 3,451 articles were found, 43 of which were analyzed. Most measures were digital, stemmed from public institutions, focused on the local perspective, and were integrated with the public health care system. This study discusses the models of care in mental health based on measures to cope with the COVID-19 pandemic. It also discusses the Brazilian health care system, reiterating its resilience. In conclusion, digital measures occurred most often. This study suggest the evaluation of the accessibility of this mental health care model for most vulnerable groups. Finally, this research reinforces the importance of the Brazilian health care system for public health and access to information to cope with the COVID-19 pandemic. COVID19 COVID 19 COVID-1 pandemic SciELO, SciELO (SciELO PubMed LILACS Portuguese English Spanish SAÚDE MENTAL SALUD HEALTH “COVID-19 202 2021 total 3451 3 451 3,45 found 4 analyzed institutions perspective resilience conclusion often groups Finally COVID1 1 COVID- “COVID-1 20 345 45 3,4 “COVID- 2 34 3, “COVID

Resumo A presente revisão integrativa tem por objetivo identificar os arranjos de cuidado em saúde mental que foram implementados no enfrentamento à pandemia de COVID-19. Realizou-se busca em três bases de dados (SciELO, PubMed e LILACS), em português, inglês e espanhol, com os descritores “SAÚDE MENTAL” or “SALUD MENTAL” or “MENTAL HEALTH” AND “COVID-19”, no período de 2020 a 2021. Foram encontrados 3.451 artigos, sendo 43 selecionados para análise. Em relação ao cuidado em saúde mental, os principais arranjos identificados foram os digitais, de natureza pública, desenvolvidos na esfera municipal e com integração com a rede de saúde. Os modelos de cuidado em saúde mental para o enfrentamento da pandemia são discutidos a partir dos tipos de arranjo produzidos nesse contexto sanitário emergencial e crítico. Apresenta-se, ainda, um recorte da realidade encontrada no Sistema Único de Saúde (SUS), reiterando sua resiliência. Concluiu-se que os arranjos digitais foram os mais usados e que há necessidade de investigar a acessibilidade deste modelo para populações com maior vulnerabilidade social. Reafirma-se a importância do SUS para o enfrentamento da COVID-19 e no acesso a informações de saúde.

Abstract This integrative review aims to identify the mental health care measures that were produced during the COVID-19 pandemic. This research was conducted on three databases (SciELO, PubMed, and LILACS) with the following descriptors in Portuguese, English, and Spanish: “SAÚDE MENTAL” or “SALUD MENTAL” or “MENTAL HEALTH” AND “COVID-19” from 2020 to 2021. In total, 3,451 articles were found, 43 of which were analyzed. Most measures were digital, stemmed from public institutions, focused on the local perspective, and were integrated with the public health care system. This study discusses the models of care in mental health based on measures to cope with the COVID-19 pandemic. It also discusses the Brazilian health care system, reiterating its resilience. In conclusion, digital measures occurred most often. This study suggest the evaluation of the accessibility of this mental health care model for most vulnerable groups. Finally, this research reinforces the importance of the Brazilian health care system for public health and access to information to cope with the COVID-19 pandemic.

BACKGROUND Leishmania tarentolae is a non-pathogenic species found in lizards representing an important model for Leishmania biology. However, several aspects of this Sauroleishmania remain unknown to explain its low level of virulence. OBJECTIVES We reported several aspects of L. tarentolae biology including glycoconjugates, proteolytic activities and metabolome composition in comparison to pathogenic species (Leishmania amazonensis, Leishmania braziliensis, Leishmania infantum and Leishmania major). METHODS Parasites were cultured for extraction and purification of lipophosphoglycan (LPG), immunofluorescence probing with anti-gp63 and resistance against complement. Parasite extracts were also tested for proteases activity and metabolome composition. FINDINGS Leishmania tarentolae does not express LPG on its surface. It expresses gp63 at lower levels compared to pathogenic species and, is highly sensitive to complement-mediated lysis. This species also lacks intracellular/extracellular activities of proteolytic enzymes. It has metabolic differences with pathogenic species, exhibiting a lower abundance of metabolites including ABC transporters, biosynthesis of unsaturated fatty acids and steroids, TCA cycle, glycine/serine/threonine metabolism, glyoxylate/dicarboxylate metabolism and pentose-phosphate pathways. MAIN CONCLUSIONS The non-pathogenic phenotype of L. tarentolae is associated with alterations in several biochemical and molecular features. This reinforces the need of comparative studies between pathogenic and non-pathogenic species to elucidate the molecular mechanisms of virulence during host-parasite interactions. nonpathogenic non However L glycoconjugates amazonensis braziliensis major. major . major) LPG, , (LPG) antigp63 antigp anti gp anti-gp6 complement surface gp6 complementmediated mediated lysis intracellularextracellular intracellular extracellular enzymes transporters steroids cycle glycineserinethreonine glycine serine threonine glyoxylatedicarboxylate glyoxylate dicarboxylate pentosephosphate pentose phosphate pathways features hostparasite host parasite interactions (LPG antigp6 anti-gp

Resumo O envelhecimento tem impacto direto no equilíbrio devido a alterações da resposta sensorial e motora, sarcopenia e redução da força muscular e da amplitude de movimento que ocorre nos idosos. Nesse contexto, programas de treinamento de força (TF) são vistos como valiosa estratégia para minimizar o efeito deletério do envelhecimento na produção de força e no equilíbrio dessa população. O objetivo do estudo foi avaliar o efeito de 12 semanas de TF com intensidade progressiva sobre as variáveis de equilíbrio estático em idosos. Participaram do estudo 23 idosos com idade média de 65±8,61 anos, de ambos os sexos, que realizaram um programa de TF de doze semanas, com frequência de três vezes por semana e com intensidade progressiva (60 - 85% de 1-RM). O equilíbrio foi avaliado antes e após o período de intervenção, por meio de avaliação estabilométrica em plataforma de força. Após a intervenção houve redução na amplitude anteroposterior (p=0,01), na velocidade anteroposterior (p=0,01) e na área total deslocada (p= 0,04). Conclui-se que o treinamento de força pode ser utilizado como ferramenta chave para minimizar o efeito deletério do envelhecimento na manutenção do equilíbrio estático. motora contexto (TF população 1 2 65861 65 8 61 65±8,6 anos sexos 60 (6 85 1RM. 1RM RM . 1-RM) p=0,01, p001 p p=0,01 , 0 01 (p=0,01 p= (p 0,04. 004 0,04 04 0,04) Concluise Conclui se 6586 6 65±8, ( 1-RM p00 p=0,0 (p=0,0 00 0,0 658 65±8 p0 p=0, (p=0, 0, 65± p=0 (p=0

Abstract Aging has a direct impact on balance due to changes in sensory and motor response, sarcopenia, reduced muscle strength and range of motion that occur in the elderly. In this context, strength training (ST) programs are seen as a valuable strategy to minimize the deleterious effect of aging on strength production and balance in this population. The purpose of the study was to evaluate the effect of 12 weeks of progressive intensity ST on static balance variables in the elderly. The study included 23 elderly with an average age of 65±8.61 years, of both sexes, who performed a twelve-week strength training program, with a frequency of three times a week and with progressive intensity (60 - 85% of 1-RM). Balance was assessed before and after the intervention period, through stabilometric assessment on a force plate. After the intervention, there was a reduction in the anteroposterior amplitude (p=0.01), in the anteroposterior velocity (p=0.01) and in the total displaced area (p=0.04). It is concluded that the strength training can be used as a key tool to minimize the deleterious effect of aging on the maintenance of static balance. response sarcopenia context (ST population 1 2 65861 65 8 61 65±8.6 years sexes twelveweek twelve program 60 (6 85 1RM. 1RM RM . 1-RM) period plate p=0.01, p001 p p=0.01 , 0 01 (p=0.01 p=0.04. p004 p=0.04 04 (p=0.04) 6586 6 65±8. ( 1-RM p00 p=0.0 (p=0.0 (p=0.04 658 65±8 p0 p=0. (p=0. 65± p=0 (p=0 p= (p= (p

Biocomposites have gained attention in the packaging industry due to their potential as sustainable alternatives to conventional synthetic materials. In this study, novel cotton incorporated poly(lactic acid)/thermoplastic starch biocomposites were developed for packaging applications using in short shelf life products the extrusion method. Pelletized samples obtained by extrusion were stamped from plates obtained by compression and were characterized through measurement of density, hardness, contact angle and water absorption, as well as Fourier transform infrared spectroscopy (FTIR), thermal analysis and scanning electron microscopy (SEM). No significant changes in the density results were observed. A slight increase in the hardness of formulations in relation to the PLA was associated with the presence of cotton fiber in biocomposites. The FTIR results revealed physical interaction of PLA, TPS and cotton fiber. By DSC analysis, for all formulations the melting exhibited only one peak, suggesting good homogeneity and interaction among the components, as observed by TG/DTG results, and corroborating SEM analysis. The biocomposite PLA/TPS/Cotton 85/10/5 wt.% displayed greater increase in water absorption than both 95/5/0 and 90/5/5 wt.% formulations, which can be attributed to the increase in starch proportion, confirming the contact angle results. The hydrophilic tendency corroborated the biodegradation process in the packaging end-of-life. materials study polylactic poly lactic acid/thermoplastic acidthermoplastic acid /thermoplastic thermoplastic method FTIR, , (FTIR) SEM. . (SEM) peak components TGDTG TG DTG PLATPSCotton Cotton 85105 85 10 5 85/10/ wt wt. 9550 95 0 95/5/ 9055 90 90/5/ proportion endoflife. endoflife end life. end-of-life (FTIR (SEM 8510 8 1 85/10 955 9 95/5 905 90/5 851 85/1 95/ 90/ 85/

RESUMO Objetivo: Avaliar a correlação entre a alcoolemia e a gravidade das lesões avaliadas pelo Índice de Gravidade da Lesão ( Injury Severity Score* – ISS) em vítimas de acidentes de trânsito internadas no Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HC-FMUSP).* Método: Estudo transversal realizado entre julho de 2018 e junho de 2019, no Pronto Socorro Central do HC-FMUSP. Foram incluídas 172 vítimas de acidentes de trânsito. Amostras de sangue foram analisadas pelo Laboratório de Toxicologia da FMUSP. Resultados: 36 pacientes (20,9%) apresentaram alcoolemia positiva (≥ 0,2 g/L), com valor médio de 1,21 g/L. No geral, os pacientes tinham uma idade média de 37,2 anos, e 136 (79,1%) eram homens. O ISS da casuística total foi 15,6; quanto à causa externa, a motocicleta ficou em primeiro lugar com 100 casos (58,1%); e os condutores foram prevalentes entre as vítimas (57,4%). Conclusão: Não houve correlação entre a gravidade das lesões e a alcoolemia das vítimas de acidente de trânsito internadas em um hospital de referência. Nível de Evidência II, Estudo de Corte Transversal. Objetivo Score HCFMUSP.* HCFMUSP HC FMUSP .* (HC-FMUSP). Método 201 2019 HCFMUSP. HC-FMUSP 17 Resultados 3 20,9% 209 20 9 (20,9% ≥ 02 0 2 0, g/L, gL g/L , g L g/L) 121 1 21 1,2 geral 372 37 37, anos 13 79,1% 791 79 (79,1% homens 15,6 156 15 6 externa 10 58,1% 581 58 (58,1%) 57,4%. 574 57,4% . 57 4 (57,4%) Conclusão referência II Transversal (HC-FMUSP) 20,9 (20,9 12 1, 79,1 7 (79,1 15, 58,1 5 (58,1% 57,4 (57,4% (HC-FMUSP 20, (20, 79, (79, 58, (58,1 57, (57,4 (20 (79 (58, (57, (2 (7 (58 (57 (5

ABSTRACT Objective: To evaluate the correlation between blood alcohol levels and the severity of injuries assessed by the Injury Severity Score (ISS) in patients who were victims of traffic accidents admitted to the Hospital das Clínicas of the Faculty of Medicine of the University of São Paulo (HCFMUSP). Methods: Cross-sectional study carried out between July 2018 and June 2019, at the Central Emergency Room of the Hospital das Clínicas of the Faculty of Medicine of the University of São Paulo (PSC-HCFMUSP). A total of 172 hospitalized patients victims of traffic accidents were included in this study. Blood samples were analyzed by the FMUSP Toxicology Laboratory. Results: 36 patients (20.9%) had positive BAC (≥ 0.2 g/L) with a mean of 1.21 g/L. Overall, patients had a mean age of 37.2 years old, and 136 (79.1%) were men. The ISS of the total casuistry was 15.6; regarding the external cause, the motorcycle was ranked first with 100 cases (58.1%), and drivers were the majority with 57.4% of the sample. Conclusion: There was no correlation between the severity of the injuries and the blood alcohol levels of traffic accident victims admitted to a reference hospital. Level of Evidence II, Cross-Sectional Study. Objective (ISS HCFMUSP. HCFMUSP . (HCFMUSP) Methods Crosssectional Cross sectional 201 2019 PSCHCFMUSP. PSCHCFMUSP PSC (PSC-HCFMUSP) 17 Laboratory Results 3 20.9% 209 20 9 (20.9% ≥ ( 02 0 2 0. g/L gL g L 121 1 21 1.2 Overall 372 37 37. old 13 79.1% 791 79 (79.1% men 15.6 156 15 6 cause 10 58.1%, 581 58.1% , 58 (58.1%) 574 57 4 57.4 sample Conclusion hospital II CrossSectional Sectional Study (HCFMUSP (PSC-HCFMUSP 20.9 (20.9 12 1. 79.1 7 (79.1 15. 58.1 5 (58.1% 57. 20. (20. 79. (79. 58. (58.1 (20 (79 (58. (2 (7 (58 (5

Abstract Tomato cultivation is globally significant, demanding enhanced yields and biotic stress resilience for sustainability. Dwarf plant utilization in tomato genetic enhancement offers underexplored benefits. Yet, Santa Cruz dwarf tomato germplasm is unavailable. This study evaluated genetic dissimilarity, agronomic potential, and acylsugar content of Santa Cruz dwarf tomato plants across three successive backcrossings. Twelve advanced backcrossing populations (BC) and a commercial control (cv. Kada) were assessed, totaling 15 treatments. Agronomic traits and acylsugar content were measured, and analysis techniques were applied to assess genetic dissimilarity and backcrossing superiority. BC3 populations UFU-Sci#8, UFU-Sci#6, UFU-Sci#5, and UFU-Sci#1 excelled. Dwarf plants predominantly exhibited high leaflet acylsugar levels, suggesting potential for acquisition of hybrid with pest resistance. Notably, UFU MC TOM1 (dwarf plant) displayed significant glycine and L-serine presence, associated with various biotic stresses. These findings unveil the promising creation of dwarf Santa Cruz tomato hybrids with a broad spectrum of resistance. sustainability benefits Yet unavailable backcrossings BC (BC cv. cv (cv Kada assessed 1 treatments measured superiority UFUSci#8, UFUSci8 UFUSci Sci#8, Sci 8 UFU-Sci#8 UFUSci#6, UFUSci6 Sci#6, 6 UFU-Sci#6 UFUSci#5, UFUSci5 Sci#5, 5 UFU-Sci#5 UFUSci#1 UFUSci1 Sci#1 UFU-Sci# excelled levels resistance Notably TOM Lserine L serine presence stresses UFUSci#8 Sci8 Sci#8 UFUSci#6 Sci6 Sci#6 UFUSci#5 Sci5 Sci#5 UFUSci# Sci1 Sci# UFU-Sci

Resumo Neste artigo apresentamos um ensaio teórico no contexto do recém-nascido projeto de pesquisa Uso Humanizado de Tecnologias Digitais na Educação Matemática: escuta, autoria e colaboração, o qual apelidamos de Matemática Digital Humanizada (MaDHu). Seu objetivo é apresentar as compreensões sobre escuta, autoria e colaboração que encontramos na literatura, identificar quais delas estão alinhadas ao Projeto MaDHu, e articulá-las teoricamente. Para isso, realizamos uma busca por trabalhos que abordassem cada um destes aspectos no contexto do uso de Tecnologias Digitais em Educação Matemática, e os apresentamos em uma revisão de literatura. Seguindo uma abordagem qualitativa de pesquisa, identificamos os conceitos de escuta ativa, autoria, e colaboração que consideramos que se alinham ao projeto MaDHu, tanto considerando os debates e vivências que influenciaram em sua criação, como parte do seu referencial teórico. A partir destas identificações, tecemos articulações entre estes conceitos, identificando sua interdependência e o papel fundamental da escuta ativa para a ocorrência da autoria e da colaboração. recémnascido recém nascido MaDHu. MaDHu . (MaDHu) literatura articulálas articulá las teoricamente isso criação identificações (MaDHu

Abstract In this article we present a theoretical essay in the context of the newborn research project Humanized Use of Digital Technologies in Mathematics Education: listening, authorship and collaboration, which we call Humanized Digital Mathematics (MaDHu). Its objective is to present the understandings about listening, authorship and collaboration that we find in the literature, identify which of them are aligned with the MaDHu Project, and articulate them theoretically. In this regard, we carried out a search for researches that addressed each of these aspects in the context of the use of Digital Technologies in Mathematics Education, and we present in a literature review. Following a qualitative research approach, we identified the concepts of active listening authorship, and collaboration which are aligned with the MaDHu project, both considering the debates and experiences that influenced its creation, as part of its theoretical framework. Based on these identifications, we developed connections between these concepts, and we identified their interdependence and the fundamental role of active listening for the occurrence of authorship and collaboration. Education MaDHu. . (MaDHu) Project theoretically regard review approach creation framework identifications (MaDHu

ABSTRACT. The use of dwarf lines to obtain mini-tomato hybrid plants has led to agronomic and economic advantages. However, the benefits provided by dwarf parents in tomato hybrids of the Santa Cruz type remain unexplored. The aims of this study were to determine the agronomic enhancement in BC1F2 dwarf populations bearing characteristic fruit of the Santa Cruz type after the first backcross and to select populations with high agronomic potential and fruit quality. The experiment was conducted in a greenhouse using a randomized block design with 15 treatments and 4 replicates. Evaluated genetic materials included 11 BC1F2 dwarf tomato populations, both parents (recurrent and donor), and the cultivars (Santa Cruz Kada and Santa Clara). Traits evaluated included: mean weight, soluble solid concentration, number of locules, shape, pulp thickness, fruit longitudinal and transversal diameters, internode length, and plant height. Univariate, multivariate, correlation, and selection index analyses showed that mean fruit weight, transverse diameter, and pulp thickness increased significantly in dwarf populations after a single backcross, most of them exhibiting a fruit shape similar to that of the Santa Cruz type. The dwarf BC1F2 populations UFU-Sci#11 and UFU-Sci#12 showed high potential for obtaining lines and, subsequently, Santa Cruz type hybrids.

BACKGROUND A positive Trypanosoma cruzi polymerase chain reaction (PCR) is associated with a worse prognosis in patients with chronic Chagas disease (CD). OBJECTIVES To study the association of clinical, electrocardiographic, and echocardiographic characteristics and biomarker blood levels with positive T. cruzi PCR in chronic CD. METHODS This is a single-centre observational cross-sectional study. Positive T. cruzi PCR association with clinical, electrocardiographic, and echocardiographic characteristics, and biomarker blood levels were studied by logistic regression analysis. p values < 0.05 were considered significant. FINDINGS Among 333 patients with chronic CD (56.4% men; 62 ± 10 years), T. cruzi PCR was positive in 41.1%. Stepwise multivariate logistic regression showed an independent association between positive T. cruzi PCR and diabetes mellitus {odds ratio (OR) 0.53 [95% confidence interval (CI) 0.30-0.93]; p = 0.03}, right bundle branch block [OR 1.78 (95% CI 1.09-2.89); p = 0.02], and history of trypanocidal treatment [OR 0.13 (95% CI 0.04-0.38); p = 0.0002]. Among patients with a history of trypanocidal treatment (n = 39), only four (10%) patients had a positive T. cruzi PCR. MAIN CONCLUSIONS Among several studied parameters, only diabetes mellitus, right bundle branch block, and history of trypanocidal treatment showed an independent association with positive T. cruzi PCR. History of trypanocidal treatment was a strong protective factor against a positive T. cruzi PCR. (PCR . (CD) clinical electrocardiographic T singlecentre single centre crosssectional cross sectional analysis 005 0 05 0.0 significant 33 56.4% 564 56 4 (56.4 men 6 1 years, years , years) 411 41 41.1% odds OR (OR 053 53 0.5 95% 95 [95 (CI 0.300.93 030093 0.30 0.93 30 93 0.30-0.93] 0.03, 003 0.03 03 0.03} 178 78 1.7 (95 1.092.89 109289 1.09 2.89 09 2 89 1.09-2.89) 0.02, 002 0.02 02 0.02] 013 13 0.1 0.040.38 004038 0.04 0.38 04 38 0.04-0.38) 0.0002. 00002 0.0002 0002 0.0002] n 39, 39 39) 10% (10% parameters (CD 00 0. 3 56.4 5 (56. 41.1 9 [9 300 0.300.9 03009 030 0.3 093 0.9 0.30-0.93 17 7 1. (9 092 1.092.8 10928 109 1.0 289 2.8 8 1.09-2.89 01 040 0.040.3 00403 004 038 0.04-0.38 0000 0.000 000 (10 56. (56 41. [ 0.300. 0300 0.30-0.9 ( 1.092. 1092 28 2. 1.09-2.8 0.040. 0040 0.04-0.3 0.00 (1 (5 0.300 0.30-0. 1.092 1.09-2. 0.040 0.04-0. 0.30-0 1.09-2 0.04-0 0.30- 1.09- 0.04-

BACKGROUND Dendritic cells (DCs) specific intercellular adhesion molecule (ICAM)-3-grabbing non integrin receptor (DC-SIGN) binds to subgenera Leishmania promastigotes mediating its interaction with DC and neutrophils, potentially influencing the infection outcome. OBJECTIVES In this work, we investigated whether DC-SIGN receptor is expressed in cells from cutaneous leishmaniasis (CL) lesions as well as the in vitro binding pattern of Leishmania (Viannia) braziliensis (Lb) and L. (L.) amazonensis (La) promastigotes. METHODS DC-SIGN receptor was labeled by immunohistochemistry in cryopreserved CL tissue fragments. In vitro binding assay with CFSE-labeled Lb or La promastigotes and RAJI-transfecting cells expressing DC-SIGN (DC-SIGNPOS) or mock-transfected (DC-SIGNNEG) were monitored by flow cytometry at 2 h, 24 h and 48 h in co-culture. RESULTS In CL lesion infiltrate, DC-SIGNPOS cells were present in the dermis and near the epidermis. Both Lb and La bind to DC-SIGNPOS cells, while binding to DC-SIGNNEG was low. La showed precocious and higher affinity to DC-SIGNhi population than to DC-SIGNlow, while Lb binding was similar in these populations. CONCLUSION Our results demonstrate that DC-SIGN receptor is present in L. braziliensis CL lesions and interact with Lb promastigotes. Moreover, the differences in the binding pattern to Lb and La suggest DC-SIGN can influence in a difference way the intake of the parasites at the first hours after Leishmania infection. These results raise the hypothesis that DC-SIGN receptor could participate in the immunopathogenesis of American tegumentary leishmaniasis accounting for the differences in the outcome of the Leishmania spp. infection. DCs (DCs ICAM3grabbing ICAMgrabbing ICAM 3 grabbing DCSIGN SIGN (DC-SIGN neutrophils work (CL Viannia (Viannia (Lb L (L. (La fragments CFSElabeled CFSE RAJItransfecting RAJI transfecting DCSIGNPOS SIGNPOS (DC-SIGNPOS mocktransfected mock transfected DCSIGNNEG SIGNNEG (DC-SIGNNEG 4 coculture. coculture co culture. culture co-culture infiltrate epidermis low DCSIGNhi SIGNhi DCSIGNlow, DCSIGNlow SIGNlow, SIGNlow DC-SIGNlow populations Moreover spp (L

ABSTRACT. The influence of harvest method on proximate composition, protein oxidation, and texture profile of beef from Nellore cattle were investigated. Longissimus lumborum (LL) muscles were obtained from twelve grain-fed Nellore carcasses. The animals were slaughtered after stunning (STU; n=6) or without stunning (WST; n=6) and after 24 hours postmortem, the LL was sliced into 2.54-cm steaks, packed under aerobic conditions, and stored at 4°C for nine days. Proximate composition was analyzed on day 0, whereas protein oxidation (carbonyl content) and texture profile (hardness, chewiness, cohesiveness, and springiness) were evaluated on days 0, 3, 6, and 9. STU and WST steaks exhibited similar proximate composition, hardness, and chewiness (p > 0.05). WST steaks exhibited greater protein oxidation and lower cohesiveness than their STU counterparts on day 0 (p < 0.05). Concerning the storage period, WST steaks demonstrated a decrease in springiness and a more pronounced increase in cohesiveness than their STU counterparts (p < 0.05). These results indicated that the harvest method influenced LL protein oxidation and texture profile from Bos indicus cattle. ABSTRACT investigated (LL grainfed grain fed carcasses (STU n=6 n6 n 6 (WST 2 postmortem 2.54cm 254cm cm 2.54 54 conditions 4C C 4 carbonyl content hardness (hardness 3 9 p 0.05. 005 0.05 . 05 0.05) period n= 54cm 254 2.5 5 00 0.0 25 2. 0.

BACKGROUND Schistosomiasis is a neglected tropical disease caused by trematodes of the genus Schistosoma, with a limited treatment, mainly based on the use of praziquantel (PZQ). Currently, several aspartic proteases genes have already been identified within the genome of Schistosoma species. At least one enzyme encoded from this gene family (SmAP), named SmCD1, has been validated for the development of schistosomicidal drugs, since it has a key role in haemoglobin digestion by worms. OBJECTIVE In this work, we integrated a structure-based virtual screening campaign, enzymatic assays and adult worms ex vivo experiments aiming to discover the first classes of SmCD1 inhibitors. METHODS Initially, the 3D-structures of SmCD1, SmCD2 and SmCD3 were generated using homology modelling approach. Using these models, we prioritised 50 compounds from 20,000 compounds from ChemBridge database for further testing in adult worm aqueous extract (AWAE) and recombinant SmCD1 using enzymatic assays. FINDINGS Seven compounds were confirmed as hits and among them, two compounds representing new chemical scaffolds, named 5 and 19, had IC50 values against SmCD1 close to 100 μM while presenting binding efficiency indexes comparable to or even higher than pepstatin, a classical tight-binding peptide inhibitor of aspartyl proteases. Upon activity comparison against mammalian enzymes, compound 50 was selective and the most potent against the AWAE aspartic protease activity (IC50 = 77.7 μM). Combination of computational and experimental results indicate that compound 50 is a selective inhibitor of SmCD2. Compounds 5, 19 and 50 tested at low concentrations (10 uM) were neither cytotoxic against WSS-1 cells (48 h) nor could kill adult worms ex-vivo, although compounds 5 and 50 presented a slight decrease on female worms motility on late incubations times (48 or 72 h). MAIN CONCLUSION Overall, the inhibitors identified in this work represent promising hits for further hit-to-lead optimisation. treatment PZQ. PZQ . (PZQ) Currently species SmAP, SmAP , (SmAP) SmCD drugs structurebased structure campaign Initially 3Dstructures Dstructures 3D structures D approach models 20000 20 000 20,00 (AWAE them scaffolds IC IC5 10 pepstatin tightbinding tight enzymes (IC5 777 77 7 77. μM. μM) 1 (1 uM WSS1 WSS WSS- 48 (4 h exvivo, exvivo vivo, ex-vivo h. Overall hittolead hit lead optimisation (PZQ (SmAP 2000 2 00 20,0 (IC ( 4 200 0 20,

BACKGROUND The parasite Giardia duodenalis infects a wide range of vertebrate hosts, including domestic and wild animals as well as humans. Giardia is genotyped into eight assemblages (A-H). Zoonotic assemblages A and B have already been identified in humans and wild and domestic animals (non-human primates and cats) from Brazilian Amazon and in the world. Due to its zoonotic/zooanthroponotic nature, surveillance initiatives and the definition of Giardia assemblages are important in order to characterise the epidemiological scenario and to implement further control measures. OBJECTIVES Determine assemblages of G. duodenalis in sloths from the Brazilian Amazon Region. METHODS Faecal parasitological examination of sloths from Amazonas State. Polymerase chain reaction (PCR) targeting the beta giardin (BG), and genes from multilocus sequence typing (MLST) scheme, amplicon sequencing and phylogenetic analysis. FINDINGS Here, we identified, by microscopy, Giardia in two northern sloths (Bradypus tridactylus). These two samples were submitted to molecular assays and it was revealed that both were infected by G. duodenalis assemblage A. Phylogenetic analysis showed that they belong to assemblage A within sequences from humans and wild and domestic animals. CONCLUSION Therefore, besides showing, by the first time, the current presence of this parasite in sloths, our findings reveals that this wild animal species would be part of the zoonotic/zooanthroponotic scenario of this parasite in the Brazilian Amazon. hosts AH. AH H . (A-H) nonhuman non human cats world zoonoticzooanthroponotic zoonotic zooanthroponotic nature measures G Region State PCR (PCR BG, BG , (BG) MLST (MLST scheme Here microscopy Bradypus tridactylus. tridactylus tridactylus) Therefore showing time (A-H (BG

ABSTRACT Sporotrichosis is the most frequent subcutaneous mycosis in Latin America. It is caused by species of the genus Sporothrix. Infection in humans occurs through the entry of the fungus into the skin. Zoonotic outbreaks involving cats in the transmission of the disease have been frequently reported. The lymphocutaneous form is the most commonly observed and the upper limbs are the most affected sites. We report a case of a 64-year-old healthy female patient with a lymphocutaneous form with rapid progression of lesions, which was refractory to initial treatment with itraconazole. Treatment with liposomal amphotericin B was performed with a satisfactory resolution, but aesthetic and functional sequelae in the left upper limb were installed. America Sporothrix skin reported sites 64yearold yearold 64 year old lesions itraconazole resolution installed 6