The emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) stands for being the most serious epidemic (so far) of the 21st century. However, only a few computational studies have investigated the molecular mechanisms underlying the neutralization of the spike protein by antibodies of different classes. Hence, bioinformatic methods were employed to unravel the factors contributing to the remarkable neutralization capacity exhibited by specific antibodies. Initially, crystallographic structures of IgA monomeric / dimeric, IgG, and IgM antibodies binding with the receptor-binding domain region of the SARS-CoV-2 spike protein were retrieved. Subsequently, rigid molecular docking and molecular dynamic simulations were performed over 100 ns with explicit water solvation. Lastly, an energy decomposition was conducted to estimate the binding affinity using the last frames from molecular dynamics. The results revealed a higher binding affinity for both monomeric and dimeric forms of IgA antibodies against the spike protein. Additionally, a greater number of hydrogen bonds were observed during their interaction with the spike protein, as well as greater structural instability along the time and especially a more thermodynamically favorable interaction affinity. In this way, the research contributes a small piece to the complex puzzle of understanding the humoral immune response induced by the SARS-CoV-2 virus. SARSCoV2 SARSCoV SARS CoV (SARS-CoV-2 so far st century However classes Hence Initially IgG receptorbinding receptor SARS-CoV- retrieved Subsequently 10 solvation Lastly dynamics Additionally way virus (SARS-CoV- SARS-CoV 1 (SARS-CoV

RESUMO O aporte de resíduos vegetais ao longo dos ciclos da cana-de-açúcar favorece o incremento de matéria orgânica e atividade de microrganismos no solo, especialmente nas camadas superficiais. A textura do solo também tem um efeito importante nos processos ecológicos e na qualidade do solo. Nesse sentido, o objetivo desta pesquisa foi avaliar atributos biológicos do solo em diferentes ciclos de cultivo de cana-de-açúcar sob colheita mecanizada, em um Latossolo da região de cerrado do Brasil Central. O estudo foi realizado em áreas comerciais de cultivo de cana-de-açúcar na safra 2018/2019, consideradas homogêneas em termos de condições edafoclimáticas, tendo como fonte de variação entre as áreas os ciclos de cultivo (C1: um ciclo de cultivo; C3: três ciclos de cultivo; C7: sete ciclos de cultivo) e uma área de vegetação de cerrado selecionada como referência. Foram determinadas variáveis microbiológicas nas camadas de 0-0,10 e 0,10-0,20 m. As variáveis relacionados à biomassa microbiana e textura foram submetidos à análise de componentes principais. Áreas com ciclos de cultivo de cana-de-açúcar mais longos apresentaram maior proporção de carbono da biomassa microbiana no carbono orgânico total nas camadas subterrâneas (quociente microbiano). O desempenho da comunidade microbiana do solo, expresso pelos indicadores carbono orgânico total e nitrogênio da biomassa microbiana foi associado à maior presença de argila e silte, ou seja, partículas de solo menores que 0,02 mm. canadeaçúcar cana açúcar superficiais sentido mecanizada Central 20182019 2018 2019 2018/2019 edafoclimáticas C1 C (C1 C3 C7 referência 00,10 0010 0 0,10 10 0-0,1 0,100,20 010020 0,20 20 0,10-0,2 m principais quociente microbiano. microbiano . microbiano) silte seja 002 02 0,0 mm 2018201 201 2018/201 (C 00 00,1 001 010 0,1 1 0-0, 100 0,100,2 01002 020 0,2 2 0,10-0, 0, 201820 2018/20 00, 01 0-0 0,100, 0100 0,10-0 20182 2018/2 0- 0,100 0,10- 2018/

ABSTRACT The contribution of plant residues throughout the sugarcane cycles favors the increase of organic matter and the activity of microorganisms in the soil, especially in the surface layers. Soil texture also has an important effect on ecological processes and soil quality. In this context, the objective of this study was to evaluate soil biological attributes in different sugarcane cultivation cycles under mechanized harvesting in an Oxisol in the Savanna region of Central Brazil. The study was conducted in commercial areas under sugarcane cultivation during the 2018/2019 season, which were considered homogeneous in terms of soil and climatic conditions, with the source of variation among the areas being the cultivation cycles (C1: one cultivation cycle; C3: three cultivation cycles; C7: seven cultivation cycles) and a savanna vegetation area selected as a reference. Microbiological variables were determined in two layers, 0-0.1 and 0.1-0.2 m. The variables related to microbial biomass and texture were subjected to principal component analysis. Areas with longer sugarcane cultivation cycles show higher proportion of microbial biomass carbon in the total organic carbon in subsurface layers (microbial quotient). The performance of the soil microbial community, as expressed by total organic carbon and microbial biomass nitrogen indicators, was associated with higher presence of clay and silt, i.e., soil particles smaller than 0.02 mm. quality context Brazil 20182019 2018 2019 2018/201 season conditions C1 C (C1 cycle C3 C7 reference 00.1 001 0 0.1 1 0-0. 0.10.2 0102 0.2 2 0.1-0. m analysis quotient. quotient . quotient) community indicators silt ie i e i.e. 002 02 0.0 mm 2018201 201 2018/20 (C 00 00. 01 0. 0-0 10 0.10. 010 0.1-0 i.e 201820 20 2018/2 0- 0.10 0.1- 20182 2018/

Resumo Introdução: O papel da tomografia computorizada torácica de ultrabaixa dose (ULDCT) em contexto de urgência é desconhecido. Objetivo: Este estudo avaliou a viabilidade da ULDCT em adultos na urgência com suspeita de pneumonia. Foram medidos a dose de radiação, o tempo entre a solicitação e os relatórios do exame, prescrições de antimicrobianos também foram correlacionadas. Métodos: Este estudo avaliou a viabilidade da ULDCT em adultos na urgência com suspeita de pneumonia. Foram medidas a dose de radiação, o tempo entre a solicitação e a disponibilização dos relatórios do exame, e também foram correlacionadas as prescrições de antimicrobianos. Entre outubro de 2017 e dezembro de 2018, incluímos prospectivamente adultos com suspeita de pneumonia adquirida na comunidade, sem diagnóstico definitivo por avaliação clínica. Os critérios de exclusão abrangeram condições clínicas que pudessem afetar a interpretação da imagem. Resultados: Foram incluídos 131 pacientes elegíveis (idade média de 43,5 anos; 57% homens) encaminhados para ULDCT. Dezoito (13,7%) foram excluídos. A dose média de radiação da ULDCT foi de 0,14 mSv. O intervalo de tempo entre a solicitação médica e o resultado da ULDCT foi de 43 minutos. Conclusão: A ULDCT mostrou-se viável para pacientes adultos com suspeita de pneumonia em contexto de urgência. Os relatórios foram disponibilizados num intervalo razoável e os resultados negativos foram associados a uma baixa taxa de prescrição de antibióticos.

Abstract Background: The role of ultra-low-dose chest CT (ULDCT) in the emergency department is unknown. Purpose: This study sought to examine feasibility of ULDCT in adults presenting to the emergency department for whom pneumonia was suspected. Radiation dose and time interval between medical request and ULDCT results were measured, antibiotic prescriptions were correlated. Methods: Between October 2017 and December 2018, we prospectively enrolled consecutive healthy adult patients in a single-center for whom community-acquired pneumonia was suspected and for whom no definitive diagnosis was possible by clinical judgment. Exclusion criteria comprised all chronic conditions that could impact severity and interpretation of image. Results: 131 eligible patients (mean age 43.5 years; 57% men) were enrolled to ULDCT. Eighteen (13.7%) patients were excluded. Average CT radiation dose was 0.14 mSv. Time interval between medical request and ultra-low-dose chest CT result was 43 minutes. Only 15.9% of patients with negative CT received antibiotics. Conclusion: Ultra-low-dose chest CT was feasible for almost all patients in a group of healthy adults presenting to the emergency department with suspected community-acquired pneumonia. Results were made available in a reasonable interval, and negative ones were associated with a low antibiotic prescription rate.

RESUMO Os resíduos da mineração de vermiculita, embora de baixa solubilidade, contêm nutrientes como cálcio, magnésio e potássio em concentrações que permitem seu uso agrícola. Bactérias benéficas podem aumentar a solubilidade deste resíduo e sua eficiência. Objetivou-se avaliar a influência da bioinoculação do resíduo de vermiculita, associado ao esterco bovino, na liberação de nutrientes e no crescimento inicial do milho. Foi realizado delineamento inteiramente casualizado em casa de vegetação com sete tratamentos e quatro repetições. Os tratamentos foram: T1 - solo sem adubação; T2 - solo adubado com fontes solúveis de nutrientes (fertilização convencional); T3 - aplicação de resíduo de vermiculita (VR); T4 - VR enriquecido com esterco bovino (CM); T5 -VR+CM com Bacillus subtilis; T6 - VR + CM + B. amyloliquefaciens e T7 - VR + CM + B. subtilis + B. amyloliquefaciens). O VR aumentou a disponibilidade de Ca e K no solo, porém seu desempenho foi inferior às fontes solúveis. Independentemente da utilização de esterco bovino ou de microrganismos benéficos, os resíduos da mineração de vermiculita aumentaram a disponibilidade de Ca e K no solo, mas não foram capazes de igualar as fontes solúveis de nutrientes. Os resíduos de mineração associados ao esterco bovino e aos microrganismos benéficos (B. subtilis e B. amyloliquefaciens) aumentaram a atividade microbiana e aumentaram a liberação de cálcio disponível para o milho. Os resíduos da mineração de vermiculita aumentaram o crescimento e a produção de massa seca do milho em comparação ao solo não fertilizado, mas foram consistentemente inferiores ao fertilizante convencional. agrícola eficiência Objetivouse Objetivou se repetições T adubação fertilização convencional convencional) (VR) (CM) VR+CM VRCM B amyloliquefaciens. . (B fertilizado (VR (CM

ABSTRACT Vermiculite mining residues, although of low solubility, contain nutrients such as calcium, magnesium, and potassium at concentrations that allow their agricultural use. Beneficial bacteria can increase the solubility of this waste and its efficiency. The objective of the present study was to evaluate the influence of bio-inoculation of vermiculite residue, associated with cattle manure, on the release of nutrients and initial growth of maize. A completely randomized design was performed in a greenhouse with seven treatments and four replications. The treatments were: T1 - soil without fertilization; T2 - soil fertilized with soluble sources of nutrients (conventional fertilization); T3 - application of vermiculite residue (VR); T4 - VR enriched with cattle manure (CM); T5 - VR + CM with Bacillus subtilis; T6 - VR + CM + B. amyloliquefaciens, and T7 - VR + CM +B. subtilis + B. amyloliquefaciens). Regardless of the use of cattle manure or beneficial microorganisms, vermiculite mining waste increased the availability of Ca and K in the soil, but was not able to match the soluble sources of nutrients. Mining waste associated with cattle manure and beneficial microorganisms (B. subtilis and B. amyloliquefaciens) increased microbial activity and enhanced the release of available calcium for maize. VR increased the growth and the dry mass production of maize compared to the non-fertilized soil, but was consistently inferior to conventional fertilizer. residues magnesium efficiency bioinoculation bio inoculation replications were T fertilization fertilization) (VR) (CM) B amyloliquefaciens +B amyloliquefaciens. . (B nonfertilized non fertilizer (VR (CM

RESUMO Objetivou-se avaliar o efeito da fertirrigação pulsada e contínua sobre o acúmulo e a exportação de nutrientes pelo feijãocaupi. O delineamento experimental adotado foi em blocos ao acaso com 12 tratamentos distribuídos em esquema fatorial 5 x 2+ 2, com quatro repetições, sendo cinco lâminas de reposição da ETc equivalentes (40, 60, 80, 100 e 120%), combinadas com dois tipos de aplicação de fertirrigação (pulsada e contínua), mais dois tratamentos adicionais os quais foram irrigados com lâmina de 100% da ETc de forma pulsada e contínua e adubado convencionalmente. Os nutrientes analisados foram N, P, K, Ca, Mg, S, Fe, Cu, Mn e Zn. Os maiores acúmulos e exportações de nutrientes foram obtidos com lâminas de fertirrigação pulsada menores que 100% da ETc. O tratamento que recebeu adubação convencional e irrigação pulsada com lâmina de 100% da ETc promoveu maiores acúmulos e exportação de nutrientes em detrimento da irrigação contínua. A ordem decrescente de acúmulo de nutrientes para ambos os tipos de aplicação da fertirrigação (pulsada e contínua) foi: N > K > Ca > P > Mg > S > Fe > Mn > Zn > Cu. A ordem decrescente de exportação de nutrientes pelas vagens e grãos do feijão-caupi, para aplicação da fertirrigação pulsada foi: N > P > Ca > K > Mg > S > Fe > Zn > Mn > Cu. Já a aplicação da fertirrigação contínua foi: N > P > Ca > Mg > S> K> Fe > Zn > Mn > Cu. Objetivouse Objetivou se feijãocaupi 1 2 repetições 40, 40 (40 60 80 10 120%, 120 120% , 120%) contínua, convencionalmente Cu feijãocaupi, feijão caupi, caupi feijão-caupi 4 (4 6 8 (

ABSTRACT The objective was to evaluate the effects of pulse and continuous fertigation on the accumulation and export of nutrients by cowpea. The experimental design adopted was randomized blocks with 12 treatments distributed in a 5 x 2 + 2 factorial scheme, with four replicates, with five ETc replacement depths (40, 60, 80, 100 and 120%), combined with two types of fertigation application (pulse and continuous), plus two additional treatments under pulse (control 1) and continuous (control 2) irrigation with 100% ETc depth and conventional fertilization. The analyzed nutrients were N, P, K, Ca, Mg, S, Fe, Cu, Mn and Zn. Highest accumulations and exports of nutrients were obtained with pulse fertigation depths lower than 100% ETc. Conventional fertilization and pulse irrigation with 100% ETc depth promoted greater accumulation and export of nutrients compared to continuous irrigation. The descending order of nutrient accumulation for both types of fertigation application (pulse and continuous) was: N > K > Ca > P > Mg > S > Fe > Mn > Zn > Cu. The descending order of nutrient export by cowpea pods and grains was N > P > Ca > K > Mg > S > Fe > Zn > Mn > Cu for pulse fertigation and N > P > Ca > Mg > S > K > Fe > Zn > Mn > Cu for continuous fertigation. 1 scheme replicates 40, 40 (40 60 80 10 120%, 120 120% , 120%) continuous, control 4 (4 6 8 (

The Metropolitan Region of Rio de Janeiro is one of the most populous in Brazil, besides being one of the most important routes for marketing goods through heavy vehicles. This type of vehicle is the main source of nitrogen oxides (NOx) emission into the atmosphere. To assess NOx emission factors, this pilot study used sensors to monitor in real-time the exhaust of 9 trucks from July to September 2022. To the best of our knowledge, this is the first study carried out in the city using low-cost sensors. Although there is legislation to reduce the emission of pollutants from the vehicle fleet, the results showed that 7 out of 9 trucks, exceeded the stipulated limits, reaching 6 g kWh-1. Furthermore, carrying out the maintenance of the engine of one of the vehicles decreased 60% of the NOx emission, even being an old vehicle. Thus, with this data, it was verified that the sensor performed excellently in monitoring NOx, demonstrating robust performance. This pilot study is part of a project that aims to make a long-term study of NOx emissions factors from heavy-duty diesel vehicles using sensors and other parameters. Brazil (NOx atmosphere realtime real time 2022 knowledge lowcost low cost fleet limits kWh1. kWh1 kWh 1. 1 kWh-1 Furthermore 60 Thus data performance longterm long term heavyduty duty parameters 202 kWh- 20 2

ABSTRACT Objective: To evaluate the cumulative incidence, risk factors, and outcomes of COVID-19 in patients with Cushing's disease (CD). Subjects and methods: In all, 60 patients with CD following up in our outpatient clinic answered via phone interview a questionnaire about the occurrence of COVID-19 infection documented by RT-PCR (including the diagnosis date and clinical outcome) and vaccination status. Clinical and biochemical data on disease activity (hypercortisolism) and comorbidities (obesity, diabetes mellitus, and hypertension) were obtained from the patients' electronic medical records. Risk ratios (RRs) of risk factors were obtained using univariate and multivariate analyses. Results: The cumulative incidence of COVID-19 in patients with CD during the observation period was 31.7%, which was higher than that in the general reference population (9.5%). The cumulative incidence of COVID-19 was significantly higher in patients with hypercortisolism (57% versus 17% in those without hypercortisolism, p = 0.012) and obesity (54% versus 9% in those without obesity, p < 0.001) but not in patients with hypertension or diabetes mellitus. On multivariate analysis, hypercortisolism and obesity were each independent risk factors for COVID-19 (RR 2.18, 95% CI 1.06-4.46, p = 0.033 and RR 5.19, 95% CI 1.61-16.74, p = 0.006, respectively). Conclusion: The incidence of COVID-19 in patients with CD was associated with hypercortisolism, as expected, and obesity, a novel and unexpected finding. Thus, correction of hypercortisolism and obesity should be implemented in patients with CD during the current and future COVID-19 outbreaks. Objective COVID19 COVID 19 COVID-1 Cushings Cushing s CD. . (CD) methods all 6 RTPCR RT PCR including outcome status (hypercortisolism (obesity mellitus records RRs (RRs analyses Results 317 31 7 31.7% 9.5%. 95 9.5% 9 5 (9.5%) 57% 57 (57 17 0.012 0012 0 012 54% 54 (54 0.001 0001 001 analysis 218 2 18 2.18 1.064.46, 106446 1.06 4.46, 1 06 4 46 1.06-4.46 0033 033 0.03 519 5.19 1.6116.74, 1611674 1.61 16.74, 61 16 74 1.61-16.74 0006 006 0.006 respectively. respectively respectively) Conclusion expected finding Thus outbreaks COVID1 COVID- (CD 3 31.7 9.5 (9.5% (5 0.01 01 0.00 000 00 21 2.1 064 1.064.46 10644 106 1.0 446 4.46 1.06-4.4 003 03 0.0 51 5.1 6116 1.6116.74 161167 161 1.6 1674 16.74 1.61-16.7 31. 9. (9.5 ( 2. 1.064.4 1064 10 1. 44 4.4 1.06-4. 0. 5. 611 1.6116.7 16116 167 16.7 1.61-16. (9. 1.064. 4. 1.06-4 1.6116. 1611 16. 1.61-16 (9 1.064 1.06- 1.6116 1.61-1 1.611 1.61-

RESUMO Este estudo teve como objetivo analisar as características físicas dos ovos de galinhas caipiras var. Suras. Foram analisados um total de 100 ovos, onde diversos parâmetros físicos, tais como pesagem do ovo inteiro, medição do diâmetro equatorial e altura do ovo, classificação da cor da casca, miragem para verificação da presença de fendas na casca, determinação da altura da câmara de ar, classificação da cor da gema, verificação da presença de manchas de carne e manchas de sangue, medição da largura e comprimento do albúmen, medição do diâmetro da gema, pesagem da gema, e pesagem da casca. Foram posteriormente determinados o peso do albúmen por diferença entre o peso do ovo e pesos da casca e gema, determinação percentual dos diferentes constituintes do ovo, shape index (índice de forma) e shell index (índice de casca). A análise estatística foi realizada pelo GLM programa SAS (SAS Institute). O resultado comparando as médias de cada parâmetro estudado demonstrou que os ovos da variedade Sura em ambas as classes comerciais (Grande e Pequenos), possuem formato mais arredondado, com casca marrom clara e gema mais alaranjada. Portanto, os parâmetros avaliados confirmam que os ovos provenientes de galinhas var. Suras possuem características que podem favorecer nichos de mercado e assim incentivar a produção destas aves, mantendo a biodiversidade e contrariando a extinção das mesmas. var 10 físicos inteiro ar sangue índice forma . casca) Institute. Institute Institute) Grande Pequenos, Pequenos , Pequenos) arredondado alaranjada Portanto aves mesmas 1

ABSTRACT This study aimed to analyze the physical characteristics of eggs from free-range hens of the Sura variety. One hundred eggs underwent assessment of various physical parameters, namely, whole egg weight, equatorial diameter, egg height, shell color, presence of cracks via candling, air chamber height, yolk color, presence of flesh and blood spots, albumen width and length, yolk diameter, yolk weight, and shell weight. Additional analyses included determining the albumen weight (calculated by subtracting the weights of the shell and yolk from the total egg weight), percentages of egg components, shape index, and shell index. Statistical analyses were conducted using GLM SAS software (SAS Institute). The results revealed distinct physical traits; eggs from the Sura variety, regardless of size class (large or small), typically have a more rounded shape, light brown shells, and more orange yolks. These findings confirm that Sura variety eggs possess unique characteristics that could appeal to specific market niches, potentially promoting the production of these birds, supporting biodiversity, and aiding in the prevention of their extinction. freerange free range parameters namely diameter height color candling spots length calculated , weight) components index Institute. Institute . Institute) traits large small, small small) shells yolks niches birds biodiversity extinction

Resumo A Serra do Ibitipoca ocorre no sudeste do estado de Minas Gerais, sudeste do Brasil, e inclui um mosaico de diferentes tipos de vegetação, como parte do domínio da Mata Atlântica. Tal heterogeneidade é resultado da ocorrência de diversos ecótonos na região, considerados áreas de amortecimento essenciais para manutenção da biodiversidade e estrutura de ecossistemas adjacentes. Dada a importância destes ambientes para conservação da biodiversidade, inventários florísticos são importantes para catalogar a riqueza de plantas em áreas naturais, onde espécies e paisagens têm sido destruídas, especialmente nas últimas décadas. Para contribuir com o aumento do conhecimento sobre a Flora vascular na Serra do Ibitipoca, um inventário florístico foi conduzido em áreas privadas adjacentes ao Parque Estadual do Ibitipoca. As descobertas mais relevantes do presente estudo incluem: caracterização dos diferentes tipos de vegetação, 17 novos registros para a Flora de Minas Gerais, coleta de 288 espécies nunca registradas para o parque (80% de dissimilaridade – especialmente devido à ocorrência e tamanho de diferentes fitofisionomias entre as áreas) e presença de 31 espécies ameaçadas. Além disso, discussões sobre esforços para conservação e políticas públicas são apresentadas. Gerais Brasil vegetação Atlântica região naturais destruídas décadas incluem 1 28 80% 80 (80 3 ameaçadas disso apresentadas 2 8 (8 (

Abstract The Ibitipoca Mountains occur in southeastern Minas Gerais state, Southeast Brazil, and includes a mosaic of different vegetation types, as part of the Atlantic Forest domain. Such heterogeneity results in the occurrence of several ecotones in the region, considered essential buffer zones for maintaining biodiversity and structure among adjacent ecosystems. Given the importance of these environments for biodiversity conservation, floristic surveys are important to catalogue plant richness in natural areas, where species and landscapes have been destroyed, especially over the last decades. To contribute to increase the knowledge on the vascular Flora in the Ibitipoca Mountains, a floristic inventory was undertaken in private properties located in the boundaries of “Parque Estadual do Ibitipoca” (Ibitipoca State Park). Relevant findings of the present study include: characterization of the different vegetation types, 17 new records for the Flora of Minas Gerais, collection of 288 species never recorded in the state park (80% dissimilarity – especially due to the occurrence and size of different phytophysiognomies between these areas) and presence of 31 threatened species. In addition, discussions about conservation efforts and public policies are presented. Brazil types domain region ecosystems areas destroyed decades Parque Park. Park . Park) include 1 28 80% 80 (80 3 addition presented 2 8 (8 (

This research explores the concentration of black carbon (BC) in particulate matter (PM10 and PM2.5) from ten monitoring stations in the Metropolitan Region of Rio de Janeiro (MRRJ), Bonsucesso (BS), Botafogo (BOT), Copacabana (COP), Gavea (GAV), Gericino (GER), Lagoa (LAG), Recreio dos Bandeirantes (REC), Santa Cruz (SC), Castelo (CAS) and Urca (URC), covering a range of pollution sources (vehicular, industrial, and residential). PM samples were collected using filter units every week from January 2018 to December 2019. Results revealed high concentrations of PM10 in BS (86 ± 22 µg m3) and PM2.5 in REC (30 ± 11 µg m3). Likewise, both monitoring stations exceeded the international limits. In 2019, BC in PM10 decreased in the following order: BS > CAS > GER > BOT > SC > GAV. For 2018, BC in PM2.5 decreased as follows REC > LAG > SC, while 2019 REC > GAV > LAG > COP > URC. REC and BS have industrial and commercial activities and intense vehicular traffic. During the period of study, average BC concentrations in PM10 and PM2.5 were 3.3 ± 1.5 and 1.9 ± 0.70 µg m3, respectively. These findings indicate that BC concentrations should be monitored and regulated in locations with high levels of traffic-related air pollution for offering new insights and guiding efforts to minimize emissions and enhance public health. (BC (PM1 PM25 PM2 5 MRRJ, MRRJ , (MRRJ) BS, (BS) BOT, (BOT) COP, (COP) GAV, (GAV) GER, (GER) LAG, (LAG) REC, (REC) (SC) (CAS URC, URC (URC) vehicular, (vehicular residential. residential . residential) 201 PM1 86 (8 2 m3 m PM2. 30 (3 1 m3. Likewise limits order traffic study 33 3 3. 15 1. 19 9 070 0 70 0.7 respectively trafficrelated related health (PM (MRRJ (BS (BOT (COP (GAV (GER (LAG (REC (SC (URC 20 8 ( 07 7 0.

Abstract Objective To evaluate the influence of environmental factors and prematurity relating to juvenile dermatomyositis (JDM), its course and refractoriness to treatment. Methods A case-control study with 35 patients followed up at a tertiary hospital and 124 healthy controls, all residents of São Paulo. Patients were classified according to monocyclic, polycyclic or chronic disease courses and refractoriness to treatment. The daily concentrations of pollutants (inhalable particulate matter-PM10, sulfur dioxide-SO2, nitrogen dioxide-NO2, ozone-O3 and carbon monoxide-CO) were provided by the Environmental Company of São Paulo. Data from the population were obtained through a questionnaire. Results Fifteen patients had monocyclic courses, and 19 polycyclic/chronic courses. Eighteen patients were refractory to treatment. Maternal occupational exposure to inhalable agents (OR = 17.88; IC 95% 2.15–148.16, p = 0.01) and exposure to O3 in the fifth year of life (third tertile > 86.28μg/m3; OR = 6.53, IC95% 1.60–26.77, p = 0.01) were risk factors for JDM in the multivariate logistic regression model. The presence of a factory/quarry at a distance farther than 200 meters from daycare/school (OR = 0.22; IC 95% 0.06–0.77; p = 0.02) was a protective factor in the same analysis. Prematurity, exposure to air pollutants/cigarette smoke/sources of inhalable pollutants in the mother's places of residence and work during the gestational period were not associated with JDM. Prematurity, maternal exposure to occupational pollutants during pregnancy as well as patient's exposure to ground-level pollutants up to the fifth year of life were not associated with disease course and treatment refractoriness. Conclusion Risk factors for JDM were maternal occupational exposure and exposure to O3 in the fifth year of life. Key points Maternal exposure to occupational pollutants during pregnancy was a risk factor for Juvenile Dermatomyositis (JDM). Exposure to ozone in the fifth year of life was found to be a risk factor for JDM. Exposure to air pollutants during the gestational period was not associated with JDM, its course or refractoriness. , (JDM) casecontrol case control 3 12 controls Paulo matterPM10, matterPM10 matterPM matter PM10, PM10 PM matter-PM10 dioxideSO2, dioxideSO2 dioxideSO dioxide SO2, SO2 SO dioxide-SO2 dioxideNO2, dioxideNO2 dioxideNO NO2, NO2 NO dioxide-NO2 ozoneO3 ozoneO O ozone-O monoxideCO monoxide CO monoxide-CO questionnaire 1 polycyclicchronic 17.88 1788 17 88 95 21514816 2 15 148 16 2.15–148.16 0.01 001 0 01 third 86.28μg/m3 8628μgm3 μgm 86 28μg m3 μg m 653 6 53 6.53 IC95 1602677 60 26 77 1.60–26.77 model factoryquarry factory quarry 20 daycareschool daycare school 0.22 022 22 0.06–0.77 006077 06 0.02 002 02 analysis Prematurity pollutantscigarette cigarette smokesources smoke sources mothers mother s patient groundlevel ground level . (JDM matterPM1 PM1 matter-PM1 dioxide-SO dioxide-NO 17.8 178 8 9 2151481 14 2.15–148.1 0.0 00 86.28μg/m 8628μgm 65 5 6.5 IC9 160267 7 1.60–26.7 0.2 0.06–0.7 00607 matter-PM 17. 215148 2.15–148. 0. 6. 16026 1.60–26. 0.06–0. 0060 21514 2.15–148 1602 1.60–26 0.06–0 006 2151 2.15–14 160 1.60–2 0.06– 215 2.15–1 1.60– 0.06 21 2.15– 1.60 2.15 1.6 2.1 1. 2.

Resumen Antecedentes: El cáncer de células pequeñas (CPCP) representa el 13-15% del total de neoplasias primarias de pulmón. Se caracteriza por su rapidez en la tasa de crecimiento y en el desarrollo de metástasis a distancia. Objetivos: Orientar y estandarizar el tratamiento del CPCP, enfermedad limitada, en México, basado en evidencia clínica nacional e internacional. Material y métodos: Este documento se desarrolló como una colaboración entre el Instituto Nacional de Cancerología y la Sociedad Mexicana de Oncología en cumplimiento con estándares internacionales. Se integró un grupo conformado por oncólogos médicos, cirujanos oncólogos, cirujanos de tórax, radio-oncólogos y metodólogos con experiencia en revisiones sistemáticas de la literatura y guías de práctica clínica. Resultados: Se consensuaron, por el método Delphi y en reuniones a distancia, las recomendaciones en CPCP enfermedad limitada, producto de las preguntas de trabajo. Se identificó y evaluó críticamente la evidencia científica que responde a cada una de dichas preguntas clínicas, antes de incorporarla a la guía. Conclusión: Esta guía proporciona recomendaciones clínicas para el manejo de la enfermedad limitada del CPCP y durante el proceso de toma de decisiones de los clínicos involucrados con su manejo en nuestro país para mejorar la calidad de la atención clínica en estos pacientes.

Abstract Background: Small cell lung cancer (SCLC) represents 13-15% of all primary lung neoplasms and is characterized by rapid growth rate and development of distant metastases. Objectives: To guide and standardize the treatment of limited disease small cell lung cancer in Mexico based on national and international clinical evidence. Material and methods: This document was developed as a collaboration between the National Cancer Institute and the Mexican Society of Oncology in compliance with international standards. An interdisciplinary group was formed, including medical oncologists, oncological surgeons, thoracic surgeons, radiation oncologists, and methodologists with experience in systematic reviews of the literature and clinical practice guidelines. Results: Consensus recommendations were reached, both by the Delphi method and in remote meetings, for limited SCLC disease resulting from the same number of work questions. The scientific evidence that answers each of these clinical questions was identified and critically evaluated, before being incorporated into the body of evidence of the guide. Conclusion: This guide provides clinical recommendations for the management of limited disease SCLC to contribute to the decision-making process of the clinicians involved with its management in our country, hoping that this will contribute to improving the quality of clinical care in these patients.

RESUMO Espécies silvestres de maracujazeiro, devido à maior tolerância aos estresses ambientais, revelam potencial como porta-enxerto interespecífico, P. foetida é uma espécie silvestre potencialmente tolerante à salinidade. Portanto, o objetivo com a pesquisa foi avaliar os efeitos da salinidade da água de irrigação no crescimento de porta-enxertos de maracujazeiro-bravo (P. foetida L.). O experimento foi realizado em ambiente protegido. Os tratamentos consistiram na condutividade elétrica da água de irrigação (0,3; 1,0; 2,0; 3,0 e 4,0 dS m-1), distribuídos em blocos casualizados. As avaliações foram realizadas aos 7, 14, 21, 28 e 35 dias após o transplantio e consistiram em: altura e diâmetro do caule, número de folhas, tamanho médio da folha, área foliar, massas das matérias secas da parte aérea, das raízes e total. Nos porta-enxertos jovens, os efeitos deletérios da salinidade sobre o crescimento em altura e os componentes foliares foram observados sob menores condutividades elétricas, sendo a tolerância aumentada com a acréscimo da idade. Entretanto, o acúmulo de biomassa seca foi restringido com maior severidade pela salinidade com o aumento da idade dos porta-enxertos. Os porta-enxertos de P. foetida são tolerantes à salinidade e podem ser irrigados com água de até 4,0 dS m-1.

ABSTRACT Wild passion fruit species, due to the greater tolerance to environmental stresses, reveal potential as interspecific rootstocks. Passiflora foetida is a wild species potentially tolerant to salinity. Therefore, the objective with the study was to evaluate the effects of irrigation water salinity on the growth of wild passion fruit (P. foetida L.) rootstocks. The experiment was carried out in a protected environment. The treatments consisted of the electrical conductivity of the irrigation water (0.3; 1.0; 2.0; 3.0 and 4.0 dS m-1), distributed in randomized blocks. The assessments were performed at 7, 14, 21, 28 and 35 days after transplanting and consisted of: stem height and diameter, number of leaves, average leaf size, leaf area, and dry matter of shoots, roots and total. In younger rootstocks, the deleterious effects of salinity on height growth and leaf components were observed under lower electrical conductivities, with tolerance increasing with age. However, dry biomass accumulation was more severely restricted by salinity with increasing rootstock age. P. foetida rootstocks are salinity tolerant and can be irrigated with water of up to 4.0 dS m-1.

Abstract Objective: To assess factors associated with emotional changes and Hyperactivity/Inattention (HI) motivated by COVID-19 quarantine in adolescents with immunocompromising diseases. Methods: A cross-sectional study included 343 adolescents with immunocompromising diseases and 108 healthy adolescents. Online questionnaires were answered including socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and validated surveys: Strengths and Difficulties Questionnaire (SDQ), Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0). Results: The frequencies of abnormal emotional SDQ scores from adolescents with chronic diseases were similar to those of healthy subjects (110/343 [32%] vs. 38/108 [35%], p = 0.548), as well as abnormal hyperactivity/inattention SDQ scores (79/343 [23%] vs. 29/108 [27%], p = 0.417). Logistic regression analysis of independent variables associated with abnormal emotional scores from adolescents with chronic diseases showed: female sex (Odds Ratio [OR = 3.76]; 95% Confidence Interval (95% CI) 2.00–7.05; p < 0.001), poor sleep quality (OR = 2.05; 95% CI 1.08–3.88; p = 0.028) and intrafamilial violence during pandemic (OR = 2.17; 95% CI 1.12–4.19; p = 0.021) as independently associated with abnormal emotional scores, whereas total PedsQL score was inversely associated with abnormal emotional scores (OR = 0.95; 95% CI 0.93–0.96; p < 0.0001). Logistic regression analysis associated with abnormal HI scores from patients evidenced that total PedsQL score (OR = 0.97; 95% CI 0.95–0.99; p = 0.010], changes in medical appointments during the pandemic (OR = 0.39; 95% CI 0.19–0.79; p = 0.021), and reliable COVID-19 information (OR = 0.35; 95% CI 0.16–0.77; p = 0.026) remained inversely associated with abnormal HI scores. Conclusion: The present study showed emotional and HI disturbances in adolescents with chronic immunosuppressive diseases during the COVID-19 pandemic. It reinforces the need to promptly implement a longitudinal program to protect the mental health of adolescents with and without chronic illnesses during future pandemics. Objective HyperactivityInattention Hyperactivity Inattention (HI COVID19 COVID 19 COVID-1 Methods crosssectional cross sectional 34 10 sociodemographic socio demographic selfrated self rated surveys SDQ, , (SDQ) PSQI, PSQI (PSQI) 40 4 0 4. PedsQL4.0. PedsQL40 PedsQL4.0 . PedsQL4 (PedsQL4.0) Results 110/343 110343 110 (110/34 32% 32 [32% vs 38108 38 38/10 35%, 35 35% [35%] 0.548, 0548 0.548 548 0.548) hyperactivityinattention hyperactivity inattention 79/343 79343 79 (79/34 23% 23 [23% 29108 29 29/10 27%, 27 27% [27%] 0.417. 0417 0.417 417 0.417) Odds OR 3.76 376 3 76 3.76] 95 (95 2.00–7.05 200705 2 00 7 05 0.001, 0001 0.001 001 0.001) 2.05 205 1.08–3.88 108388 1 08 88 0.028 0028 028 2.17 217 17 1.12–4.19 112419 12 0.021 0021 021 0.95 095 0.93–0.96 093096 93 96 0.0001. 00001 0.0001 0.0001) 0.97 097 97 0.95–0.99 095099 99 0.010, 0010 0.010 010 0.010] 0.39 039 39 0.19–0.79 019079 0.021, 0.35 035 0.16–0.77 016077 16 77 0.026 0026 026 Conclusion pandemics COVID1 COVID- (SDQ (PSQI PedsQL4. (PedsQL4.0 110/34 11034 11 (110/3 [32 3810 38/1 [35% 054 0.54 54 79/34 7934 (79/3 [23 2910 29/1 [27% 041 0.41 41 3.7 37 9 (9 2.00–7.0 20070 000 0.00 2.0 20 1.08–3.8 10838 8 0.02 002 02 2.1 21 1.12–4.1 11241 0.9 09 0.93–0.9 09309 0000 0.000 0.95–0.9 09509 0.01 01 0.3 03 0.19–0.7 01907 0.16–0.7 01607 (PedsQL4. 110/3 1103 (110/ [3 381 38/ [35 0.5 5 79/3 793 (79/ [2 291 29/ [27 04 0.4 3. ( 2.00–7. 2007 0.0 2. 1.08–3. 1083 1.12–4. 1124 0. 0.93–0. 0930 0.95–0. 0950 0.19–0. 0190 0.16–0. 0160 (PedsQL4 110/ (110 [ 79/ (79 2.00–7 200 1.08–3 1.12–4 112 0.93–0 093 0.95–0 0.19–0 019 0.16–0 016 (PedsQL (11 (7 2.00– 1.08– 1.12– 0.93– 0.95– 0.19– 0.16– (1 2.00 1.08 1.12 0.93 0.19 0.16 1.0 1.1 0.1 1.