Variations in lipid profile have been observed in sickle cell disease (SCD) and understanding their relationship with disease severity is crucial. This study aimed to investigate the association of polymorphisms of the CETP gene and laboratory markers of disease severity with lipid profile in a pediatric population with SCD. Biochemical and anthropometric analyses and CETP and alpha-thalassemia genotyping were performed. The study included 133 children and adolescents with sickle cell anemia (SCA) or hemoglobin SC disease (SCC), in steady-state. The SCA and no hydroxyurea (no HU) groups had higher values of ApoB, total cholesterol, low-density lipoprotein cholesterol (LDL-C), and non-high-density lipoprotein cholesterol (non-HDL-C) compared to the SCC and HU groups. However, there were no significant differences in ApoA1 and HDL-C levels between the groups based on genotype. Furthermore, the groups with altered levels of ApoA1, HDL-C, and the triglyceride/HDL ratio exhibited lower hemoglobin (Hb) levels and higher white blood cell counts. Hb level was associated to HDL-C levels. Analysis of CETP gene variants showed that the minor alleles of rs3764261 (C>A), rs247616 (C>T), and rs183130 (C>T), as well as the TTA haplotype, are explanatory variables for HDL-C levels. These findings suggested that dyslipidemia in SCD, specifically related to HDL-C levels, may be influenced by individual genetic background. Additionally, further investigation is needed to determine if clinical manifestations are impacted by CETP gene variants. SCD (SCD crucial alphathalassemia alpha thalassemia performed 13 (SCA SCC, , (SCC) steadystate. steadystate steady state. state steady-state ApoB lowdensity low density LDLC, LDLC LDL C (LDL-C) nonhighdensity non high nonHDLC HDL (non-HDL-C However ApoA HDLC genotype Furthermore HDLC, C, triglycerideHDL triglyceride (Hb counts rs rs376426 C>A, CA C>A A (C>A) rs24761 C>T, CT C>T T (C>T) rs18313 haplotype background Additionally 1 (SCC (LDL-C rs37642 (C>A rs2476 (C>T rs1831 rs3764 rs247 rs183 rs376 rs24 rs18 rs37 rs2 rs1 rs3

Integrated farming is an established agricultural practice aimed at increasing productivity per unit area by utilizing animal manure. However, there has been a growing concern regarding swine manure's potential as a disseminator of pathogens in the environment. Among these pathogens is Porcine Circovirus 2 (PCV2), which can persist in the environment for extended periods. The objective of this study is to demonstrate the impact of introducing two PCV2 vaccination protocols on PCV2 load in faecal samples and the surrounding environment. To achieve this, fifty-seven pigs aged 21 d were divided into three groups (A, B, and Control group) and received intramuscular injections of two different PCV2 vaccines as per the manufacturer's instructions. Faecal and environmental samples were collected using polyester swabs and tested for PCV2 using SYBR green quantitative real-time PCR (qPCR). The introduction of vaccination in a PCV2-positive herd led to a reduction in viral load in both faecal samples and the surrounding environment.

RESUMO Este estudo apresenta uma descrição macroscópica do plexo braquial na preguiça-real, Choloepus didactylus, e na preguiça-comum, Bradypus variegatus, a fim de identificar os pontos de bloqueio anestésico para realização de procedimentos cirúrgicos. Seis C. didactylus e nove B. variegatus tiveram seus membros torácicos dissecados, expondo-se o plexo braquial, e posteriormente localizados os pontos de bloqueio. Em geral, a formação do plexo braquial em C. didactylus envolveu nervos C5-T1, enquanto o plexo braquial em alguns indivíduos da espécie envolveu C4-T2. Em B. variegatus, o plexo braquial foi constituído por C6-T2. Em ambas as espécies, as ramificações deram origem a três troncos que se uniram para formar um tronco comum do plexo braquial. Na técnica de bloqueio do plexo braquial, foram realizados quatro acessos: supraclavicular, infraclavicular, axilar e distal. Os resultados deste estudo contribuem para procedimentos cirúrgicos e anestésicos e pesquisas sobre anatomia comparativa entre animais silvestres. preguiçareal, preguiçareal preguiça real, real preguiça-real preguiçacomum, preguiçacomum comum, preguiça-comum C B dissecados expondose expondo geral C5T1, C5T1 CT C5 T1, T1 T C5-T1 C4T2. C4T2 C4 T2. T2 C4-T2 C6T2. C6T2 C6 C6-T2 espécies acessos supraclavicular infraclavicular distal silvestres C5T C5-T C4T C4-T C6T C6-T

ABSTRACT This study presents a macroscopic description of the brachial plexus in the royal sloth, Choloepus didactylus, and the common sloth, Bradypus variegatus, to identify the points for anesthetic block to perform surgical procedures. Six C. didactylus and nine B. variegatus had their forelimbs dissected, exposing the brachial plexus, and the points for blockage were located subsequently. In general, the formation of the brachial plexus in C. didactylus involved C5-T1 nerves, whereas the brachial plexus in a few individuals of the species involved C4-T2. In B. variegatus, the brachial plexus was contributed by C6-T2. In both species, the ramifications gave rise to three trunks that joined together to form a common brachial plexus trunk. In the brachial plexus block technique, four accesses were performed: supraclavicular, infraclavicular, axillary, and distal. The results of this study contribute to surgical and anesthetic procedures and research on comparative anatomy among wild animals. sloth C B dissected subsequently general C5T1 CT C5 T1 T C5-T nerves C4T2. C4T2 C4 T2. T2 C4-T2 C6T2. C6T2 C6 C6-T2 trunk technique performed supraclavicular infraclavicular axillary distal animals C5T C4T C4-T C6T C6-T

RESUMO Chumbo é um metal pesado e uma causa importante de toxicose crônica no homem e em animais domésticos e selvagens. Este relato teve como objetivo descrever um caso de intoxicação crônica por chumbo em uma onça-pintada (Panthera onca), mantida sob cuidados humanos, que foi resgatada de seu ambiente natural em 2004, em pobre condição corporal. O animal foi encaminhado ao zoológico de Belo Horizonte (MG, Brasil), onde permaneceu até 2020, quando desenvolveu anorexia, vômitos e ataxia. Durante os últimos anos, o animal teve episódios de anemia e aumento da concentração sérica de ureia e creatinina. O exame radiográfico demonstrou 21 projéteis radiodensos na face direita. À necropsia, foram observados vários projéteis envoltos por tecido conjuntivo no subcutâneo da face esquerda, peritonite fibrinosa, múltiplas úlceras gástricas e melena. A concentração de chumbo foi determinada por espectrometria de absorção atômica em amostras de tecido renal, indicando 908 ppb (µg/kg). O achado de projéteis de chumbo associado à elevada concentração de chumbo é compatível com intoxicação crônica por chumbo neste caso. selvagens onçapintada onça pintada Panthera onca, onca , onca) humanos 2004 corporal MG, MG (MG Brasil, Brasil Brasil) 2020 anorexia ataxia anos creatinina 2 direita necropsia esquerda fibrinosa melena renal 90 µg/kg. µgkg µg/kg . µg kg (µg/kg) 200 202 9 (µg/kg 20

ABSTRACT Lead is a heavy metal and an important cause of acute or chronic toxicosis in humans, domestic, and wild animals. This report aims to describe a case of chronic lead poisoning in a jaguar (Panthera onca) kept under human care that was rescued from the wild environment. The animal was rescued in poor condition in 2004 and kept under human care at the Belo Horizonte Zoological Garden (Minas Gerais, Brazil) until 2020, when it presented with anorexia, vomiting and ataxia. Over the past years the animal had episodes of anemia and increased serum urea and creatinine. Radiography demonstrated 21 radiopaque projectiles on the right side of the face. At necropsy there were multiple projectiles surrounded by fibrous tissue in the subcutaneous of the right side of the face, fibrinous peritonitis, multiple gastric ulcers, and melena. The lead dosage was performed using the atomic absorption spectrometry technique using renal tissue collected at necropsy, with a result of 908 ppb (µg/kg). The findings of projectiles associated with the dosage of lead above the reference limits allow the diagnosis of chronic intoxication in this case. humans domestic animals Panthera onca environment 200 Minas Gerais Brazil 2020 anorexia ataxia creatinine 2 face peritonitis ulcers melena 90 µg/kg. µgkg µg/kg . µg kg (µg/kg) 20 202 9 (µg/kg

Abstract This study was conducted to examine the effects of a diet formulated with industrial amino acids and a commercial vitamin-mineral mixture on the characteristics of carcass and meat cuts of slow-growing broilers slaughtered at different ages. The experiment involved 600 one-day-old male and female white Naked Neck chicks. The chicks were allotted randomly to a 2 × 3 factorial arrangement consisting of two diets and three slaughter ages, in a completely randomized experimental design with five replicates of 20 birds each. The experimental period was 84 days. Six chicks were selected and slaughtered on the first day, and then at 56, 70 and 84 days of age 10 birds unit was selected and slaughtered per experimental period. Weight and yield of carcass, abdominal fat and breast, thigh and drumstick meat were determined. Additionally, moisture, protein, fat and mineral matter contents and protein and fat deposition in the breast, drumstick and thigh muscles were determined. The diet did not influence the yields of carcass, abdominal fat, or meat cuts or the nutritional composition of meat. Slaughter age influenced the yields of breast and thigh meat and abdominal fat; the protein content of breast meat; and the moisture, crude protein, fat, and mineral matter contents of drumstick and thigh meat. There was an interaction effect between diet and slaughter age for protein deposition in breast meat. The age factor influenced fat deposition in the drumstick muscles. Protein deposition in the thigh muscles was influenced by the diet. Moreover, a difference was observed between the ages for protein and fat deposition in the thigh muscle. In conclusion, the use of a commercial mixture in the diet results in decreased protein deposition in the breast and thigh muscles, although this difference does not affect the characteristics of carcass or meat cuts. Birds slaughtered at 70 days exhibit similar carcass characteristics to those of birds slaughtered at 84 days, indicating the possibility of an earlier slaughter age. vitaminmineral vitamin slowgrowing slow growing 60 onedayold one day old each 8 56 7 1 determined Additionally moisture Moreover muscle conclusion 6 5

RESUMO: Este estudo teve como objetivo identificar a presença de DNA de Trypanosoma vivax no colostro de cabras infectadas experimentalmente e verificar a possibilidade de transmissão para neonatos alimentados com colostro coletado de cabras infectadas. Foram utilizadas doze cabras no terço final de gestação com idade aproximada de 24 meses. Seis cabras foram inoculadas intravenosamente com 0,5mL de sangue contendo aproximadamente 1,25x105 tripomastigotas de T. vivax, e seis permaneceram não infectadas. A presença de T. vivax no colostro foi avaliada por Reação em Cadeia da Polimerase (PCR). A possibilidade de transmissão de T. vivax pelo colostro foi avaliada através da alimentação de seis neonatos nascidos de cabras sorologicamente negativas com colostro de cabras infectadas. Foi coletado diariamente o sangue periférico dos neonatos, por trinta dias para avaliar a presença de T. vivax através do exame de esfregaços de camadas leucocitárias coradas por giemsa, pela técnica BCT e por PCR. Os resultados do exame direto do colostro foram negativos, mas a PCR confirmou a presença de DNA de T. vivax no colostro em todas as cabras infectadas. Além disso, a transmissão lactogênica pelo colostro não foi demonstrada, uma vez que tanto a BCT quanto a PCR do sangue periférico do neonato foram negativas. RESUMO 2 meses 05mL mL 0 5mL 125x105 x 1 25x105 1,25x10 T . (PCR) giemsa negativos disso demonstrada 125x10 25x10 1,25x1 (PCR 125x1 25x1 1,25x 125x 25x

ABSTRACT: This study aimed to identify the presence of Trypanosoma vivax DNA in the colostrum of infected goats and to explore the possibility of transmission for neonates fed using colostrum collected from infected goats. We used twelve goats in the final third of gestation with an age of approximately 24 months. Six goats were inoculated intravenously with 0.5mL of blood containing approximately 1.25x105 trypomastigotes of T. vivax, and six remained uninfected. The presence of T. vivax in colostrum was evaluated by Polymerase Chain Reaction (PCR). The possibility of T. vivax transmission by colostrum was assessed by feeding six neonates born of serologically negative goats using colostrum from infected goats. Peripheral blood from neonates was collected daily for thirty days to assess the T. vivax presence through the examination of Giemsa-stained smears of leukocyte layers with the buffy coat technique (BCT) and by PCR. The results of a direct examination of colostrum were negative, but PCR confirmed the presence of T. vivax DNA in all infected goats. Additionally, lactogenic transmission by colostrum was not demonstrated once both BCT and PCR of neonate peripheral blood were negative. ABSTRACT 2 months 05mL mL 0 5mL 125x105 x 1 25x105 1.25x10 T uninfected . (PCR) Giemsastained Giemsa stained (BCT Additionally 125x10 25x10 1.25x1 (PCR 125x1 25x1 1.25x 125x 25x

The COVID-19 pandemic has challenged the entire world, and patients with diabetes mellitus (DM) have been particularly affected. We aimed to evaluate predictors of mortality during the first 30 days of hospitalization in critically ill patients with COVID-19 and comorbid DM. This prospective study included 110 critically ill patients admitted with COVID-19 infection. Thirty-two (29%) patients had a previous diagnosis of DM. Clinical variables, laboratory tests, and vascular biomarkers, such as VCAM-1, syndecan-1, ICAM-1, angiopoietin-1, and angiopoeitin-2, were evaluated after intensive care unit (ICU) admission. A comparison was made between patients with and without DM. No difference in mortality was observed between the groups (48.7 vs 46.9%, P=0.861). In the multivariate Cox regression analysis, VCAM-1 levels at ICU admission (HR: 1 [1-1.001], P<0.006) were associated with death in patients with DM. Among patients with DM, advanced age (HR 1.063 [1.031-1.096], P<0.001), increased Ang-2/Ang-1 ratio (HR: 4.515 [1.803-11.308] P=0.001), and need for dialysis (HR: 3.489 [1.409-8.642], P=0.007) were independent predictors of death. Higher levels of VCAM-1 in patients with DM was better at predicting death of patients with severe COVID-19 and comorbid DM, and their cut-off values were useful for stratifying patients with a worse prognosis. Vascular biomarkers VCAM-1 and Ang-2/Ang-1 ratio were predictors of death in patients with severe COVID-19 and comorbid DM and those without DM. Additionally, kidney injury was associated with an increased risk of death. COVID19 COVID 19 COVID-1 world (DM affected 3 11 infection Thirtytwo Thirty two 29% 29 (29% variables tests VCAM1, VCAM1 VCAM 1, syndecan1, syndecan1 syndecan syndecan-1 ICAM1, ICAM1 ICAM ICAM-1 angiopoietin1, angiopoietin1 angiopoietin angiopoietin-1 angiopoeitin2, angiopoeitin2 angiopoeitin 2, 2 angiopoeitin-2 (ICU 48.7 487 48 7 (48. 469 46 9 46.9% P=0.861. P0861 P P=0.861 . 0 861 P=0.861) analysis VCAM- HR 11.001, 11001 1.001 , 001 [1-1.001] P<0.006 P0006 006 1063 063 1.06 1.0311.096, 10311096 1.031 1.096 031 096 [1.031-1.096] P<0.001, P0001 P<0.001 P<0.001) Ang2/Ang1 Ang2Ang1 AngAng Ang 2/Ang Ang-2/Ang- 4515 4 515 4.51 1.80311.308 180311308 1.803 11.308 803 308 [1.803-11.308 P=0.001, P=0.001 P=0.001) 3489 489 3.48 1.4098.642, 14098642 1.409 8.642 409 8 642 [1.409-8.642] P=0.007 P0007 007 cutoff cut off prognosis Additionally COVID1 COVID- (29 syndecan- ICAM- angiopoietin- angiopoeitin- 48. (48 46.9 P086 P=0.86 86 11.001 1100 1001 1.00 00 [1-1.001 P<0.00 P000 106 06 1.0 0311 1.0311.096 1031109 1031 1.03 1096 1.09 03 09 [1.031-1.096 Ang2 Ang1 Ang2/Ang Ang2Ang 2Ang Ang-2/Ang 451 51 4.5 80311 1.80311.30 18031130 1803 1.80 11308 11.30 80 [1.803-11.30 P=0.00 348 3.4 4098 1.4098.642 1409864 1409 1.40 8642 8.64 40 64 [1.409-8.642 (2 (4 46. P08 P=0.8 11.00 100 [1-1.00 P<0.0 P00 10 1. 1.0311.09 103110 103 109 [1.031-1.09 45 5 4. 8031 1.80311.3 1803113 180 1.8 1130 11.3 [1.803-11.3 P=0.0 34 3. 1.4098.64 140986 140 1.4 864 8.6 6 [1.409-8.64 ( P0 P=0. 11.0 [1-1.0 P<0. 1.0311.0 10311 [1.031-1.0 1.80311. 180311 18 113 11. [1.803-11. 1.4098.6 14098 14 8. [1.409-8.6 P=0 [1-1. P<0 1.0311. [1.031-1. 1.80311 18031 [1.803-11 1.4098. [1.409-8. P= [1-1 P< 1.0311 [1.031-1 1.8031 [1.803-1 1.4098 [1.409-8 [1- [1.031- [1.803- [1.409- [1 [1.031 [1.803 [1.409 [ [1.03 [1.80 [1.40 [1.0 [1.8 [1.4 [1.

Non-invasive brain stimulation (NIBS) probing the dorsolateral prefrontal cortex (DLPFC) has been shown to have little effect on working memory. The variability of NIBS responses might be explained by inter-subject brain anatomical variability. We investigated whether baseline cortical brain thickness of regions of interest was associated with working memory performance after NIBS by performing a secondary analysis of previously published research. Structural magnetic resonance imaging data were analyzed from healthy subjects who received transcranial direct current stimulation (tDCS), intermittent theta-burst stimulation (iTBS), and placebo. Twenty-two participants were randomly assigned to receive all the interventions in a random order. The working memory task was conducted after the end of each NIBS session. Regions of interest were the bilateral DLPFC, medial prefrontal cortex, and posterior cingulate cortex. Overall, 66 NIBS sessions were performed. Findings revealed a negative significant association between cortical thickness of the bilateral dorsolateral prefrontal cortex and reaction time for both tDCS (left: P=0.045, right: P=0.037) and iTBS (left: P=0.007, right: P=0.007) compared to placebo. A significant positive association was found for iTBS and posterior cingulate cortex (P=0.03). No association was found for accuracy. Our findings provide the first evidence that individual cortical thickness of healthy subjects might be associated with working memory performance following different NIBS interventions. Therefore, cortical thickness could explain - to some extent - the heterogeneous effects of NIBS probing the DLPFC. Noninvasive Non invasive (NIBS DLPFC (DLPFC intersubject inter subject research tDCS, , (tDCS) thetaburst theta burst iTBS, (iTBS) placebo Twentytwo Twenty two order session Overall 6 performed left (left P0045 P 0 045 P=0.045 right P=0.037 P0037 037 P0007 007 P=0.007 P=0.03. P003 P=0.03 . 03 (P=0.03) accuracy Therefore (tDCS (iTBS P004 04 P=0.04 P000 00 P=0.00 P00 P=0.0 (P=0.03 P0 P=0. (P=0.0 P=0 (P=0. P= (P=0 (P= (P

Resumo Muitas cidades são impactadas pelo efeito das ilhas de calor. Neste contexto, objetivou-se com esse analisar a distribuição espacial e temporal das ilhas de calor urbanas e a influência das áreas verdes na formação dos microclimas urbanos no município do Rio de Janeiro. Como resultados, observou-se que Rio de Janeiro encontra-se sob influência das ilhas de calor, as áreas mais antigas da cidade e sobretudo mais urbanizadas são as mais acometidas pelas altas temperaturas. Foi possível constatar o papel da vegetação na formação dos microclimas urbanos por meio do coordenador de psicologia negativa entre a temperatura da superfície (Ts) e os índices de vegetação (NDVI) e (EVI), e ainda um coeficiente de transferência positiva entre Temperatura da Superfície (Ts) e Índice de Áreas Construídas por Diferença Normalizada (NDBI), demonstrando a influência da urbanização no aumento da temperatura. contexto objetivouse objetivou se resultados observouse observou encontrase encontra temperaturas Ts (Ts NDVI (NDVI EVI, EVI , (EVI) NDBI, NDBI (NDBI) (EVI (NDBI

Abstract Many cities are impacted by the effect of heat islands. In this context, the aim of this study was to analyze the spatial and temporal distribution of urban heat islands and the influence of green areas on the formation of urban microclimates in the city of Rio de Janeiro. As a result, it was observed that Rio de Janeiro city is under heat islands influence, in which the oldest and most urbanized areas of the city are the most affected by high temperatures. It was possible to verify the role of vegetation in the formation of urban microclimates through the negative correlation coefficient between surface temperature (Ts) and vegetation indexes (NDVI and EVI), along with the positive correlation coefficient between surface temperature (Ts) and the Index of Areas Built by Normalized Difference (NDBI), what demonstrates the influence of urbanization on the increase of temperature. context result temperatures Ts (Ts NDVI EVI, EVI , EVI) NDBI, NDBI (NDBI) (NDBI

Resumen Muchas ciudades se ven afectadas por el efecto de las islas de calor. En este contexto, el objetivo de este estudio fue analizar la distribución espacial y temporal de las islas de calor urbanas y la influencia de las áreas verdes en la formación de los microclimas urbanos en el municipio de Río de Janeiro, Brasil. Como resultados, se observó que Río de Janeiro está influenciada por las islas de calor, siendo las áreas más antiguas y urbanizadas las más afectadas por altas temperaturas. Se pudo constatar el papel de la vegetación en la formación de los microclimas urbanos mediante el coeficiente de correlación negativo entre la temperatura de la superficie (Ts) y los índices de vegetación (NDVI) y (EVI), así como un coeficiente de correlación positivo entre la temperatura de la superficie (Ts) y el Índice de Áreas Construidas por Diferencia Normalizada (NDBI), lo que demuestra la influencia de la urbanización en el aumento de la temperatura. contexto Brasil resultados temperaturas Ts (Ts NDVI (NDVI EVI, EVI , (EVI) NDBI, NDBI (NDBI) (EVI (NDBI

Abstract The present study investigated by palynological and chemical analysis (Flame Atomic Absortion Spectrometry) about the botanical origin and the heavy metals content (arsenic, cadmium, chromium, lead and mercury) of monthly honey samples of Apis mellifera L. over two years. The pollen types Apiaceae, Mimosa caesalpiniifolia, M. tenuiflora and Myrcia indicated the main floristic sources used by bees. M. tenuiflora was the most frequent of the pollen types, and because it predominates in different months in each year, which may indicate more than one species of the genus being foraged by the beehive. The climatic influence (rainfall and temperature) on the pollen diversity was investigated and was not statistically supported. The chemical analysis showed that the heavy metal content of the samples were below their respective limits of quantification, and, therefore, the samples can be considered safe for human consumption.

RESUMO O objetivo da presente pesquisa foi estabelecer parâmetros de referência para níveis hematológicos e bioquímicos, de cinco preguiças sadias, da espécie Bradypus variegatus (preguiça-de-garganta-marrom), em cativeiro, alimentando-se de Cecropia pachystachya (embaúba) em períodos alternados com dieta livre, no Parque Estadual de Dois Irmãos (PEDI) Recife, Pernambuco-Brasil.

ABSTRACT The aim of this study was to establish reference parameters for the hematological and biochemical levels of five healthy captive sloths of the speciesBradypus variegatus(brown-throated sloth) feeding onCecropia pachystachya(Ambay pumpwood), alternating with a period of free diet in the Dois Irmãos State Park (DISP) Recife, Pernambuco - Brazil.

RESUMO O objetivo deste estudo foi investigar a ocorrência de doenças em canídeos silvestres de vida livre que foram atropelados em rodovias no estado do Espírito Santo, Brasil. Testes de PCR foram realizados para a detecção de Brucella sp., Babesia sp., Rangelia sp. e Hepatozoon sp. no baço. A avaliação morfológica e a identificação de parasitas foram realizadaa no fígado e no pulmão. Vinte espécimes de C. thous foram necropsiados no Setor de Patologia Animal do Hospital Veterinário da Universidade Federal do Espírito Santo. Amostras de tecido foram processadas para exame histopatológico e análise de reação em cadeia da polimerase (PCR). Não houve amplificação por PCR das sequências de DNA genômico de Brucella sp., Babesia sp., Rangelia sp. e Hepatozoon sp. usando-se DNA extraído do baço como modelo. Histologicamente, lesões associadas ao parasitismo por Platynosomum sp. e Angiostrongylus sp. foram observadas no fígado e no pulmão, respectivamente. Este é o primeiro relato de Platynosomum sp. e Angiostrongylus sp. parasitismo em C. thous no estado do Espírito Santo, Brasil. Portanto, este estudo demonstrou parasitismo de cachorro-do-mato por Platynosomum sp. e Angiostrongylus sp. É importante detacar que não há evidências de infecção por Brucella sp., Babesia sp., Rangelia sp. e Hepatozoon sp. por análise de PCR.

ABSTRACT The aim of this study was to investigate the occurrence of diseases in free-ranging wild canids that were roadkill on highways in the State of Espírito Santo, Brazil. PCR tests were performed for the detection of Brucella sp., Babesia sp., Rangelia sp., and Hepatozoon sp. in the spleen. Morphological evaluation and identification of parasites was performed in the liver and lung. Twenty specimens of C. thous were necropsied at the Animal Pathology Sector of the Veterinary Hospital of the Universidade Federal do Espírito Santo. Tissue samples were processed for histopathological examination and polymerase chain reaction (PCR) analysis. There was no PCR amplification of genomic DNA sequences of Brucella sp., Babesia sp., Rangelia sp., and Hepatozoon sp. using DNA extracted from the spleen as template. Histologically, lesions associated with parasitism by Platynosomum sp. and Angiostrongylus sp. were observed in the liver and lung, respectively. This is the first report of Platynosomum sp. and Angiostrongylus sp. parasitism in C. thous in the state of Espírito Santo, Brazil. Therefore, this study demonstrated parasitism of crab-eating foxes by Platynosomum sp. and Angiostrongylus sp. Importantly, no evidence of infection with Brucella sp., Babesia sp., Rangelia sp., and Hepatozoon sp. was obtained by PCR analysis.

RESUMO A emergência de cepas de Staphylococcus aureus resistentes à meticilina associadas à pecuária (LA-MRSA) e o papel potencial dos suínos na evolução dessas cepas têm levado ao aumento do interesse na pesquisa desses microrganismos. No entanto, isso tem contribuído para a falta de estudos sobre o isolamento e a caracterização de cepas de S. aureus sensíveis à meticilina (MSSA). Neste estudo, foi analisada a prevalência de S. aureus em suínos nas fases de creche e terminação. Os perfis de suscetibilidade aos antibióticos, a tolerância a metais pesados e a produção de biofilme dos isolados foram avaliados por meio de técnicas fenotípicas e genotípicas. Um total de 1.250 colônias sugestivas de Staphylococcus spp. foi isolado de 128 suínos, das quais 63,6% (n = 795) pertenciam a esse gênero microbiano. Sessenta e sete colônias isoladas de 34 animais (26,5%) foram confirmadas como S. aureus (8,4%). Nenhuma cepa resistente ao cobre, ao zinco ou à meticilina foi detectada; entretanto, todas as cepas apresentaram perfil de resistência a pelo menos três classes diferentes de antimicrobianos e 21 produziam biofilme. Esses dados são preocupantes, pois indicam a necessidade de maior vigilância no uso de antimicrobianos, bem como reforçam a importância de estudos com cepas de MSSA.

ABSTRACT The emergence of livestock-associated methicillin-resistant Staphylococcus aureus strains (LA-MRSA) and the potential role of pigs in the evolution of these strains has led to increased interest in research of these microorganisms. However, this has contributed to a lack of research in the isolation and characterization of methicillin-susceptible S. aureus strains (MSSA). In this study, the prevalence of S. aureus in pigs in the nursery and finishing stages were analyzed. The susceptibility profiles to antibiotics, tolerance to heavy metals, and biofilm production of the isolates were evaluated using phenotypic and genotypic techniques. A total of 1,250 colonies suggestive of Staphylococcus spp. were isolated from 128 pigs, of which 63.6% (n = 795) belonged to this microbial genus. Sixty-seven colonies isolated from 34 animals (26.5%) were confirmed as S. aureus (8.4%). No strains resistant to copper, zinc, or methicillin were detected; however, all strains presented a resistance profile to at least three different classes of antimicrobials and 21 produced biofilms. These data are of concern, as they indicate the need for increased surveillance in the use of antimicrobials as well as reinforce the importance of studies on MSSA strains.

RESUMO O objetivo deste trabalho foi avaliar a produção de biomassa e qualidade bromatológica de genótipos de azevém, em dez municípios das regiões Oeste e Planalto Norte Catarinense, Estado de Santa Catarina, Brasil. Foram comparados os cultivares La Estanzuela 284 (diploide), Bar HQ, Barjumbo, INIA Escorpio, Potro e Winter Star (tetraploides), distribuídos em um delineamento blocos casualizados, em que os municípios constituíram os blocos, com três repetições. Os cortes foram realizados quando as plantas atingiram 30cm, deixando um resíduo de 10cm. Sob três cortes, os cultivares Barjumbo e Bar HQ foram os mais produtivos, ultrapassando 4,6 t ha-1 de matéria seca. Nos locais em que ocorreram cinco cortes, a produção destes cultivares superou 7,3 t ha-1, posicionando-os novamente à frente dos demais. O teor médio de proteína bruta em três cortes foi superior a 25% em todos os cultivares. Não houve diferença entre os genótipos no teor de fibra detergente neutro e de nutrientes digestíveis totais. Verificou-se correlação significativa entre variáveis produtivas quantitativas e qualitativas. Os cultivares testados representam boas opções para compor sistemas forrageiros hiberno-primaveris de curta ou longa duração, ajustando-se à integração com lavouras ou pastagens anuais de estação quente.

ABSTRACT The goal of this work was to assess the biomass production and bromatological quality of ryegrass genotypes in ten municipalities of the Western and North Plateau regions of the State of Santa Catarina, Brazil. The cultivars La Estanzuela 284 (diploid), Bar HQ, Barjumbo, INIA Escorpio, Potro, and Winter Star (tetraploids) were compared, distributed in a randomized block design, in which the municipalities constituted the blocks, with three replications. The cuts were performed when the plants reached 30cm, leaving a residue of 10cm. In three cuts, the cultivars Barjumbo and Bar HQ were the most productive, exceeding 4.6 t ha-1 of dry matter. In the places in which five cuts were performed, the production of these cultivars exceeded 7.3 t ha-1, placing them again ahead of the others. The average crude protein content in three cuts was greater than 25% in all cultivars. There was no difference between the genotypes in the content of neutral detergent fiber and total digestible nutrients. There was a significant correlation between quantitative and qualitative productive variables. The assessed cultivars represented good options for composing short-term or long-term winter-feeding systems, adjusted to the integration with annual crops or warm-season pastures.

RESUMO O teratoma é uma neoplasia rara, com diferenciação em duas ou três linhagens de células germinativas. Teratomas intracranianos, em aves, são raramente relatados, principalmente, com localização cerebral. Este é o primeiro relato de teratoma cerebral em um pato-real com sinais clínicos neurológicos. A neoplasia foi caracterizada como um teratoma cerebral maduro, estendendo do cerebelo ao tronco encefálico e com um nódulo no córtex.

ABSTRACT Teratoma is a rare neoplasia with differentiation in two or three germ cell lines. Intracranial teratoma in birds has rarely been reported, especially affecting the brain. This is the first report of a brain teratoma in a mallard with neurological clinical signs. The neoplasm was characterized as a mature brain teratoma, extending from the cerebellum to the brainstem, and with one nodule in the cortex.