Abstract Background Sowing dates and weed management practices could alter several phenological phases of direct seeded rice. However, limited is known about the impacts of these aspects on phenology, thermal efficiencies, and agro-meteorological indices of direct seeded rice. Objective The objective of the study was to evaluate the effect of sowing dates and weed management strategies on phenology, thermal efficiencies, and agro-meteorological indices of direct seeded rice. Methods Two sowing dates [10th May (early) and 3rd June (late)] and six weed management strategies [i.e., weedy-check, 4-time mechanical weeding (weed-free), bensulfuron methyl + pretilachlor as pre-emergence, oxyfluorfen as pre-emergence, bensulfuron methyl + pretilachlor as pre-emergence + 2,4-D as post-emergence, and oxyfluorfen as pre-emergence + 2,4-D as post-emergence] were included in the study. Results Early sowing took more days to reach various phenological phases, accumulated higher heat units, and recorded significantly higher thermal efficiencies, and yield than late sowing. Mechanical weeding took more time to complete different developmental stages, hence, accumulated higher heat units and recorded significantly higher thermal-use efficiencies and yield followed by application of bensulfuron methyl + pretilachlor as pre-emergence + 2,4-D as post-emergence. Conclusion Earlier sowing with 4 mechanical weedings or successive pre-emergence application of bensulfuron methyl + pretilachlor followed by post-emergence application of 2,4-D is recommended for better phenological development and yield of direct seeded rice. rice However phenology agrometeorological agro meteorological 10th th early (early rd (late) i.e., ie i e [i.e. weedycheck, weedycheck weedy check, check weedy-check 4time weedfree, weedfree free , (weed-free) preemergence, preemergence pre emergence, emergence 2,4D 24D D 2,4 2 postemergence, postemergence post stages hence thermaluse use postemergence. emergence. (late i.e. [i.e (weed-free 4D 24 2, i.e

ABSTRACT The objective of this experiment was to investigate the effect of supplementation of an exogenous emulsifier (lyso-phospholipid) in the diet of growing broilers on growth performance and digestibility. A total of 1224 Ross-308 ten day old broiler chicks were distributed into two experimental treatments in such a way that each treatment had twelve replicates with fifty-one birds per replicate. Two experimental diets were formulated with and without emulsifier supplementation according to the nutrition standards of Ross 308. Feed intake and body weight gain of the broilers were measured on a daily basis and feed conversion ratio was also calculated. Nutrient digestibility was determined on the 25th day of age. Analysis of variance under completely randomized design technique was used to analyze the data. Feed intake was increased (p<0.05) by supplementation of emulsifier in the broiler diet on theat 12th, 13th, 21th, 22th, 23th, 24th, and 25th days. Bodyweight gain was not affected (p>0.05) with or without emulsifier supplementation in the broiler diet during 11-25 days of life. However, feed conversion ratio was effected (p<0.05) by emulsifier supplementation and increased from days 21-25th. Nutrient digestibility (dry matter, fat, and crude protein) in the grower phase was decreased (p<0.05) by supplementation of emulsifier in the diet. It can be concluded that supplementation of an exogenous emulsifier in the diet did not show positive effect on the growth performance during the grower phase of broilers, while nutrient digestibility showed adverse effect. Emulsifier supplementation should be tested after 25 days of the life of broilers.

ABSTRACT The study was conducted to check the effect of emulsifier supplementation on growth performance of broiler birds on starter phase, from days 1 to 10. In the current study, a total of twelve hundred twenty-four Ross 308 broiler one day old chicks were used. Ross 308 broiler chicks were divided into two groups in such a way that each group had12 replicates, and each replicate had 51 birds. The duration of the current trial was of 10 days. Chicks were fed experimental diets with or without emulsifier supplementation. Daily feed intake, body weight, feed conversion ratio and nutrient digestibility were recorded. Mortality was recorded on a daily basis, and feed intake was also corrected for mortality. Data collected from the experiment was interpreted by using analysis of variance method using SPSSTM under Completely Randomized Design. Results showed that emulsifier supplementation improved (p<0.05) feed intake in broiler birds on the 2nd, 6th, 7th and 9th days of the starter phase. Furthermore, emulsifier supplementation (p<0.05) affected the body weight on the 3rd and 10th day of the trial. Emulsifier supplementation also affected (p<0.05) feed conversion ratio on the 10th day of the trial. However, nutrient digestibility was not affected by the supplementation of emulsifier in the diet of broiler chicks. Results showed that emulsifier supplementation should be started on day 10.

Abstract Agricultural crops suffer many diseases, including fungal and bacterial infections, causing significant yield losses. The identification and characterisation of pathogenesis-related protein genes, such as chitinases, can lead to reduction in pathogen growth, thereby increasing tolerance against fungal pathogens. In the present study, the chitinase I gene was isolated from the genomic DNA of Barley (Hordeum vulgare L.) cultivar, Haider-93. The isolated DNA was used as template for the amplification of the ∼935 bp full-length chitinase I gene. Based on the sequence of the amplified gene fragment, class I barley chitinase shares 93% amino acid sequence homology with class II wheat chitinase. Interestingly, barley class I chitinase and class II chitinase do not share sequence homology. Furthermore, the amplified fragment was expressed in Escherichia coli Rosetta strain under the control of T7 promoter in pET 30a vector. Recombinant chitinase protein of 35 kDa exhibited highest expression at 0.5 mM concentration of IPTG. Expressed recombinant protein of 35 kDa was purified to homogeneity with affinity chromatography. Following purification, a Western blot assay for recombinant chitinase protein measuring 35 kDa was developed with His-tag specific antibodies. The purified recombinant chitinase protein was demonstrated to inhibit significantly the important phytopathogenic fungi Alternaria solani, Fusarium spp, Rhizoctonia solani and Verticillium dahliae compared to the control at concentrations of 80 µg and 200 µg.

El objetivo principal de este trabajo es plantear la controversia entre Descartes y Leibniz en torno a las verdades eternas como constituyente de diversos diálogos incluyendo los contralógicos: diálogos en los cuales Descartes y Leibniz representan perspectivas distintas en relación con las elecciones posibles para la determinación de normas de racionalidad. Cada uno de estos diálogos tiene un aspecto universal o monológico (determinado por la estrategia de ganancia), y un aspecto eminentemente contextual y dialógico determinado por el nivel de juego. Finalmente, afirmo que una noción de racionalidad que contenga tanto el aspecto contextual como el universal, no es una lógica específica en forma dialógica sino un cuadro de racionalidad normativa. Lo que, según creo, está relacionado con la idea de la construcción dinámica de una lengua universal perfectible, discutida por Olga Pombo. La idea general es que la noción misma de racionalidad de Leibniz es fondateur de discursivité, si usamos la expresión que Marcelo Dascal toma de la boca de Foucault.

The main aim of the present paper is to understand the debate between Descartes and Leibniz about eternal truths as providing the structure of several possible dialogues involving counter-logicals conditionals. According to this analysis the positions of Descartes and Leibniz are understood as constituting dual and dynamic perspectives in relation to the availability of some specific choices that should provide norms of rationality. Each of these dialogues has both a universal, monological aspect (given by the winning strategy) and a contextual, dialogical one (given by the play level). I conclude with the suggestion that a notion of rationality that contains both the universal and the contextual aspects does not yield a specific logic, but rather a frame of normative rationality. The conception of such a frame seems to be closely linked to the notion of a universal perfectible language discussed by Olga Pombo. The central idea is that the Leibniz's notion of rationality is a fondateur de discursivité if we use Marcelo Dascal's quote of Foucault.